BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005389
         (1083 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002515967.1| malic enzyme, putative [Ricinus communis] gi...   575   e-162
gb|AAF73006.1|AF262997_1 NADP-dependent malic protein [Ricinus c...   575   e-162
emb|CAA56354.1| NADP dependent malic enzyme [Phaseolus vulgaris]      575   e-162
ref|XP_003526504.1| PREDICTED: NADP-dependent malic enzyme-like ...   573   e-161
gb|AAB08874.1| malate dehydrogenase [Vitis vinifera]                  572   e-161

>ref|XP_002515967.1| malic enzyme, putative [Ricinus communis] gi|223544872|gb|EEF46387.1|
            malic enzyme, putative [Ricinus communis]
          Length = 641

 Score =  575 bits (1483), Expect = e-162
 Identities = 290/360 (80%), Positives = 312/360 (86%)
 Frame = -2

Query: 1082 GRILEVLRNWPEKKIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLP 903
            GRILEVLRNWPEK IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLP
Sbjct: 218  GRILEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLP 277

Query: 902  VTIDVGTNNEDLLNDEFYIGLRRRRATGQEYAELLHEFMSAVKQNYGEKVLIQFEDFAXX 723
            VTIDVGTNNE LLNDEFYIGLR+RRATGQEYAELLHEFM+AVKQNYGE+VL+QFEDFA  
Sbjct: 278  VTIDVGTNNEKLLNDEFYIGLRQRRATGQEYAELLHEFMTAVKQNYGERVLVQFEDFAN- 336

Query: 722  XXXXXXXXXXXXXXXXXXXXXXXXXHNAFDLLAKYGATHLVFNDDIQGTASVVLAGVMAA 543
                                     HNAFDLLAKYG THLVFNDDIQGTASVVLAG++AA
Sbjct: 337  -------------------------HNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLVAA 371

Query: 542  LKLVGGTLADQKFLFLGAGEAGTGIAELIALEISKQTETPLEEARKNIHLVDSKGLIVKS 363
            LKLVGG+LAD +FLFLGAGEAGTGIAELIALE+SKQT  P+EE RK I LVDSKGLIV S
Sbjct: 372  LKLVGGSLADHRFLFLGAGEAGTGIAELIALEMSKQTNMPVEETRKKIWLVDSKGLIVSS 431

Query: 362  RLESLQQFKQPWAHEHEPIKELLDAVKAIKPTVLIGSSGVGKTFTKAVVENMSSLNEKPV 183
            R++SLQ FK+PWAHEHEPIK LLDAV  IKPTVLIG+SGVG+TFTK VVE M+S NEKP+
Sbjct: 432  RMDSLQHFKRPWAHEHEPIKTLLDAVNDIKPTVLIGTSGVGRTFTKEVVEAMASFNEKPI 491

Query: 182  IFALSNPTSQSECTAEEAYTWSKGRAIYASGSPFNPVEYNGKIYAPGQANNAYIFPGFGL 3
            I ALSNPTSQSECTAEEAYTWS+GRAI+ASGSPF PVEY GK+Y PGQANNAYIFPGFGL
Sbjct: 492  ILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKVYVPGQANNAYIFPGFGL 551


>gb|AAF73006.1|AF262997_1 NADP-dependent malic protein [Ricinus communis]
          Length = 641

 Score =  575 bits (1483), Expect = e-162
 Identities = 290/360 (80%), Positives = 312/360 (86%)
 Frame = -2

Query: 1082 GRILEVLRNWPEKKIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLP 903
            GRILEVLRNWPEK IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLP
Sbjct: 218  GRILEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLP 277

Query: 902  VTIDVGTNNEDLLNDEFYIGLRRRRATGQEYAELLHEFMSAVKQNYGEKVLIQFEDFAXX 723
            VTIDVGTNNE LLNDEFYIGLR+RRATGQEYAELLHEFM+AVKQNYGE+VL+QFEDFA  
Sbjct: 278  VTIDVGTNNEKLLNDEFYIGLRQRRATGQEYAELLHEFMTAVKQNYGERVLVQFEDFAN- 336

Query: 722  XXXXXXXXXXXXXXXXXXXXXXXXXHNAFDLLAKYGATHLVFNDDIQGTASVVLAGVMAA 543
                                     HNAFDLLAKYG THLVFNDDIQGTASVVLAG++AA
Sbjct: 337  -------------------------HNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLVAA 371

Query: 542  LKLVGGTLADQKFLFLGAGEAGTGIAELIALEISKQTETPLEEARKNIHLVDSKGLIVKS 363
            LKLVGG+LAD +FLFLGAGEAGTGIAELIALE+SKQT  P+EE RK I LVDSKGLIV S
Sbjct: 372  LKLVGGSLADHRFLFLGAGEAGTGIAELIALEMSKQTNMPVEETRKKIWLVDSKGLIVSS 431

Query: 362  RLESLQQFKQPWAHEHEPIKELLDAVKAIKPTVLIGSSGVGKTFTKAVVENMSSLNEKPV 183
            R++SLQ FK+PWAHEHEPIK LLDAV  IKPTVLIG+SGVG+TFTK VVE M+S NEKP+
Sbjct: 432  RMDSLQHFKRPWAHEHEPIKTLLDAVNDIKPTVLIGTSGVGRTFTKEVVEAMASFNEKPI 491

Query: 182  IFALSNPTSQSECTAEEAYTWSKGRAIYASGSPFNPVEYNGKIYAPGQANNAYIFPGFGL 3
            I ALSNPTSQSECTAEEAYTWS+GRAI+ASGSPF PVEY GK+Y PGQANNAYIFPGFGL
Sbjct: 492  ILALSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKVYVPGQANNAYIFPGFGL 551


>emb|CAA56354.1| NADP dependent malic enzyme [Phaseolus vulgaris]
          Length = 589

 Score =  575 bits (1481), Expect = e-162
 Identities = 290/360 (80%), Positives = 313/360 (86%)
 Frame = -2

Query: 1082 GRILEVLRNWPEKKIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLP 903
            G+ILEVL+NWPEK IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLP
Sbjct: 166  GKILEVLKNWPEKSIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLP 225

Query: 902  VTIDVGTNNEDLLNDEFYIGLRRRRATGQEYAELLHEFMSAVKQNYGEKVLIQFEDFAXX 723
            VTIDVGTNNE LLNDEFYIGLR+RRATGQEYAELL EFM AVKQNYGEKVL+QFEDFA  
Sbjct: 226  VTIDVGTNNEKLLNDEFYIGLRQRRATGQEYAELLDEFMRAVKQNYGEKVLVQFEDFAN- 284

Query: 722  XXXXXXXXXXXXXXXXXXXXXXXXXHNAFDLLAKYGATHLVFNDDIQGTASVVLAGVMAA 543
                                     HNAFDLL KY ++HLVFNDDIQGTASVVLAG++A+
Sbjct: 285  -------------------------HNAFDLLEKYSSSHLVFNDDIQGTASVVLAGLLAS 319

Query: 542  LKLVGGTLADQKFLFLGAGEAGTGIAELIALEISKQTETPLEEARKNIHLVDSKGLIVKS 363
            LKLVGGTLAD  FLFLGAGEAGTGIAELIALE+SKQT+ P+EE RK I LVDSKGLIV S
Sbjct: 320  LKLVGGTLADHTFLFLGAGEAGTGIAELIALEVSKQTKAPVEETRKKIWLVDSKGLIVSS 379

Query: 362  RLESLQQFKQPWAHEHEPIKELLDAVKAIKPTVLIGSSGVGKTFTKAVVENMSSLNEKPV 183
            RLESLQQFK+PWAHEHEP+K LL+AVKAIKPTVLIGSSG GKTFTK VVE M+SLNEKP+
Sbjct: 380  RLESLQQFKKPWAHEHEPVKGLLEAVKAIKPTVLIGSSGAGKTFTKEVVETMASLNEKPL 439

Query: 182  IFALSNPTSQSECTAEEAYTWSKGRAIYASGSPFNPVEYNGKIYAPGQANNAYIFPGFGL 3
            I ALSNPTSQSECTAEEAYTWSKGRAI+ASGSPF+PVEY GK++ PGQANNAYIFPGFGL
Sbjct: 440  ILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVEYEGKLFVPGQANNAYIFPGFGL 499


>ref|XP_003526504.1| PREDICTED: NADP-dependent malic enzyme-like [Glycine max]
          Length = 589

 Score =  573 bits (1476), Expect = e-161
 Identities = 288/360 (80%), Positives = 312/360 (86%)
 Frame = -2

Query: 1082 GRILEVLRNWPEKKIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLP 903
            G+ILEVL+NWPEK IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPS+CLP
Sbjct: 166  GKILEVLKNWPEKSIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSSCLP 225

Query: 902  VTIDVGTNNEDLLNDEFYIGLRRRRATGQEYAELLHEFMSAVKQNYGEKVLIQFEDFAXX 723
            +TIDVGTNNE LLNDEFYIGL+++RATGQEYAELL EFM AVKQNYGEKVLIQFEDFA  
Sbjct: 226  ITIDVGTNNEKLLNDEFYIGLKQKRATGQEYAELLEEFMHAVKQNYGEKVLIQFEDFAN- 284

Query: 722  XXXXXXXXXXXXXXXXXXXXXXXXXHNAFDLLAKYGATHLVFNDDIQGTASVVLAGVMAA 543
                                     HNAFDLL KY ++HLVFNDDIQGTASVVLAG++A+
Sbjct: 285  -------------------------HNAFDLLEKYSSSHLVFNDDIQGTASVVLAGLLAS 319

Query: 542  LKLVGGTLADQKFLFLGAGEAGTGIAELIALEISKQTETPLEEARKNIHLVDSKGLIVKS 363
            LKLVGGTL D  FLFLGAGEAGTGIAELIALEISKQT+ P+EE RK I LVDSKGLIV S
Sbjct: 320  LKLVGGTLVDHTFLFLGAGEAGTGIAELIALEISKQTKAPVEETRKKIWLVDSKGLIVSS 379

Query: 362  RLESLQQFKQPWAHEHEPIKELLDAVKAIKPTVLIGSSGVGKTFTKAVVENMSSLNEKPV 183
            RLESLQQFK+PWAHEHEP+K LLDAVKAIKPTVLIGSSG GKTFTK VVE M+SLNEKP+
Sbjct: 380  RLESLQQFKKPWAHEHEPVKSLLDAVKAIKPTVLIGSSGAGKTFTKEVVETMASLNEKPL 439

Query: 182  IFALSNPTSQSECTAEEAYTWSKGRAIYASGSPFNPVEYNGKIYAPGQANNAYIFPGFGL 3
            I ALSNPTSQSECTAEEAYTWSKG+AI+ASGSPF+PVEY GK++ PGQANNAYIFPGFGL
Sbjct: 440  ILALSNPTSQSECTAEEAYTWSKGKAIFASGSPFDPVEYEGKLFVPGQANNAYIFPGFGL 499


>gb|AAB08874.1| malate dehydrogenase [Vitis vinifera]
          Length = 640

 Score =  572 bits (1474), Expect = e-161
 Identities = 285/360 (79%), Positives = 314/360 (87%)
 Frame = -2

Query: 1082 GRILEVLRNWPEKKIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLP 903
            G+ILEVL+NWPEK IQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGG+RPSACLP
Sbjct: 217  GKILEVLKNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLP 276

Query: 902  VTIDVGTNNEDLLNDEFYIGLRRRRATGQEYAELLHEFMSAVKQNYGEKVLIQFEDFAXX 723
            VTIDVGTNN+ LL+DEFYIGLR++RATGQEYAEL+HEFM AVKQNYGEKVL+QFEDFA  
Sbjct: 277  VTIDVGTNNQKLLDDEFYIGLRQKRATGQEYAELIHEFMCAVKQNYGEKVLVQFEDFAN- 335

Query: 722  XXXXXXXXXXXXXXXXXXXXXXXXXHNAFDLLAKYGATHLVFNDDIQGTASVVLAGVMAA 543
                                     HNAFDLLA+YG THLVFNDDIQGTASVVLAG+++A
Sbjct: 336  -------------------------HNAFDLLARYGTTHLVFNDDIQGTASVVLAGLISA 370

Query: 542  LKLVGGTLADQKFLFLGAGEAGTGIAELIALEISKQTETPLEEARKNIHLVDSKGLIVKS 363
            L LVGGTLA+  FLFLGAGEAGTGIAELIALE+SKQT+ PLEE RK I LVDSKGLIV S
Sbjct: 371  LNLVGGTLAEHTFLFLGAGEAGTGIAELIALEMSKQTQAPLEETRKKIWLVDSKGLIVSS 430

Query: 362  RLESLQQFKQPWAHEHEPIKELLDAVKAIKPTVLIGSSGVGKTFTKAVVENMSSLNEKPV 183
            R++SLQ FK+PWAHEHEPIK L+DAVKAIKPTVLIGSSGVG+TFTK VVE M+S NEKP+
Sbjct: 431  RMDSLQHFKKPWAHEHEPIKNLVDAVKAIKPTVLIGSSGVGRTFTKEVVEAMASFNEKPI 490

Query: 182  IFALSNPTSQSECTAEEAYTWSKGRAIYASGSPFNPVEYNGKIYAPGQANNAYIFPGFGL 3
            I ALSNPTSQSECTAEEAYTWS+GRAI+ASGSPF+PVEYNGK++ PGQ+NNAYIFPGFGL
Sbjct: 491  ILALSNPTSQSECTAEEAYTWSEGRAIFASGSPFDPVEYNGKVFVPGQSNNAYIFPGFGL 550