BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005338
         (915 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEJ79821.1| beta-amyrin synthase AS2 [Eleutherococcus sentico...   567   e-159
gb|AAS01523.1| putative beta-amyrin synthase [Centella asiatica]      555   e-156
dbj|BAF63702.1| mixed amyrin synthase [Olea europaea]                 523   e-146
gb|AFJ19235.1| mixed amyrin synthase [Catharanthus roseus]            518   e-145
gb|AEX99665.1| amyrin synthase [Catharanthus roseus]                  518   e-145

>gb|AEJ79821.1| beta-amyrin synthase AS2 [Eleutherococcus senticosus]
          Length = 408

 Score =  567 bits (1462), Expect = e-159
 Identities = 258/304 (84%), Positives = 286/304 (94%)
 Frame = +3

Query: 3   IQLRQEIYPIPYDDINWNKQRHNCCKEDLYYPHSFIQDLLWDGLHYLGEPIINSWPFNKI 182
           + LRQEI+PIPY++INWNKQRHNCCKEDLYYPHSF+QDLLWDGLHYL EPII  WPFNK+
Sbjct: 32  LSLRQEIHPIPYENINWNKQRHNCCKEDLYYPHSFVQDLLWDGLHYLSEPIIKYWPFNKL 91

Query: 183 RKRGLKRAIELMRYGAEESRYITIGCVEKSLQMMCWWAENPDGDEFKHHLARVPDYLWLA 362
           R+RGL++AIELMRYGA ESRYITIGCVEKSLQMMCWWAENP+GDEFKHHLARVPDYLWLA
Sbjct: 92  RQRGLRKAIELMRYGATESRYITIGCVEKSLQMMCWWAENPNGDEFKHHLARVPDYLWLA 151

Query: 363 EDGMKMQSFGSQLWDCVLATQAIMATGMVEEYGDSLKKAHFYIKESQVKDNPSGDFESMC 542
           EDGMKMQSFGSQ+WDC LATQAI+A+ MVEEYGDSLKKAHF++KESQ+K+NPSGDFESMC
Sbjct: 152 EDGMKMQSFGSQVWDCTLATQAIIASNMVEEYGDSLKKAHFFLKESQIKENPSGDFESMC 211

Query: 543 RKFTKGSWTFSDQDQGWVVSDCTAEALKCLLSLSQMPEEIAGEKAQVERLHEAVDVLLYL 722
           R FTKGSW FSDQD GWVVSDCTAEALKCLL LS+MP EIAGEK +VERL+E V+VLLYL
Sbjct: 212 RLFTKGSWAFSDQDHGWVVSDCTAEALKCLLLLSRMPTEIAGEKTKVERLYETVNVLLYL 271

Query: 723 QSPETGGFAIWEPPVPQPYLQILNPSELFADIVVEKEHLECTASIMEALLAFKHLHHGYR 902
           QSPE+GGFAIWEPP+P+PYLQILNPSE+FADIVVEKEHLECTASI+EAL+AF  +H  YR
Sbjct: 272 QSPESGGFAIWEPPIPKPYLQILNPSEIFADIVVEKEHLECTASIIEALVAFNRIHQSYR 331

Query: 903 EKEI 914
           EKEI
Sbjct: 332 EKEI 335


>gb|AAS01523.1| putative beta-amyrin synthase [Centella asiatica]
          Length = 760

 Score =  555 bits (1429), Expect = e-156
 Identities = 257/304 (84%), Positives = 280/304 (92%)
 Frame = +3

Query: 3    IQLRQEIYPIPYDDINWNKQRHNCCKEDLYYPHSFIQDLLWDGLHYLGEPIINSWPFNKI 182
            I LR+EI+PIPY+ INWNKQRHNC KEDLYYPHSFIQDLLWDGLHY  EPII  WPFNK+
Sbjct: 285  ISLRKEIHPIPYEKINWNKQRHNCNKEDLYYPHSFIQDLLWDGLHYFTEPIIKMWPFNKL 344

Query: 183  RKRGLKRAIELMRYGAEESRYITIGCVEKSLQMMCWWAENPDGDEFKHHLARVPDYLWLA 362
            RK+G+KRAIELMRYG  ESR+ITIGCV KSL MMCWWAENP+G EFKHHLARVPDYLWLA
Sbjct: 345  RKKGMKRAIELMRYGGYESRFITIGCVSKSLDMMCWWAENPNGPEFKHHLARVPDYLWLA 404

Query: 363  EDGMKMQSFGSQLWDCVLATQAIMATGMVEEYGDSLKKAHFYIKESQVKDNPSGDFESMC 542
            EDGMKMQSFGSQLWDCVLATQA+M+TGMV+EYGD LKKAHFYIKESQ K NPSGD+ SMC
Sbjct: 405  EDGMKMQSFGSQLWDCVLATQAVMSTGMVDEYGDCLKKAHFYIKESQCKKNPSGDYASMC 464

Query: 543  RKFTKGSWTFSDQDQGWVVSDCTAEALKCLLSLSQMPEEIAGEKAQVERLHEAVDVLLYL 722
            R FTKGSWTFSDQDQGWVVSDCTAEALKCLL+LSQMPEEIAGEKA VERL++AV+VLLYL
Sbjct: 465  RYFTKGSWTFSDQDQGWVVSDCTAEALKCLLALSQMPEEIAGEKADVERLYDAVNVLLYL 524

Query: 723  QSPETGGFAIWEPPVPQPYLQILNPSELFADIVVEKEHLECTASIMEALLAFKHLHHGYR 902
            QSP +GGFAIWEPPVP+PYLQ+LNPSE+FADIVVEKEH ECTASI+ AL+AFK LH G+R
Sbjct: 525  QSPISGGFAIWEPPVPRPYLQVLNPSEIFADIVVEKEHTECTASIIAALVAFKRLHPGHR 584

Query: 903  EKEI 914
             KEI
Sbjct: 585  SKEI 588


>dbj|BAF63702.1| mixed amyrin synthase [Olea europaea]
          Length = 762

 Score =  523 bits (1347), Expect = e-146
 Identities = 240/304 (78%), Positives = 275/304 (90%)
 Frame = +3

Query: 3    IQLRQEIYPIPYDDINWNKQRHNCCKEDLYYPHSFIQDLLWDGLHYLGEPIINSWPFNKI 182
            + LR EI+  PY++I+WNK RH+CCKEDLYYPHS IQDLLWD L+Y  EP++  WP NKI
Sbjct: 285  LSLRNEIHIKPYNEIDWNKARHDCCKEDLYYPHSSIQDLLWDTLNYCAEPVMRRWPLNKI 344

Query: 183  RKRGLKRAIELMRYGAEESRYITIGCVEKSLQMMCWWAENPDGDEFKHHLARVPDYLWLA 362
            R+R L + I+ MRYGAEESRYITIGCVEKSLQMMCWWA +P+GDEFKHHLARVPDYLWLA
Sbjct: 345  RQRALNKTIKYMRYGAEESRYITIGCVEKSLQMMCWWAHDPNGDEFKHHLARVPDYLWLA 404

Query: 363  EDGMKMQSFGSQLWDCVLATQAIMATGMVEEYGDSLKKAHFYIKESQVKDNPSGDFESMC 542
            EDGMKMQSFGSQ+WD  LATQA++ATGMVEEYGD LKKAHFY+KESQ+K+NP+GDF+SM 
Sbjct: 405  EDGMKMQSFGSQIWDSTLATQAVIATGMVEEYGDCLKKAHFYVKESQIKENPAGDFKSMY 464

Query: 543  RKFTKGSWTFSDQDQGWVVSDCTAEALKCLLSLSQMPEEIAGEKAQVERLHEAVDVLLYL 722
            R FTKG+WTFSDQDQGWVVSDCTAEALKCLL LSQ+P E AGEKA VERL+EAV+VLLYL
Sbjct: 465  RHFTKGAWTFSDQDQGWVVSDCTAEALKCLLLLSQLPTETAGEKADVERLYEAVNVLLYL 524

Query: 723  QSPETGGFAIWEPPVPQPYLQILNPSELFADIVVEKEHLECTASIMEALLAFKHLHHGYR 902
            QSPE+GGFAIWEPPVPQPYLQ+LNPSE+FADIVVE EH+EC+ASI++ALLAFK L+ G+R
Sbjct: 525  QSPESGGFAIWEPPVPQPYLQMLNPSEIFADIVVETEHVECSASIIQALLAFKRLYPGHR 584

Query: 903  EKEI 914
            EKEI
Sbjct: 585  EKEI 588


>gb|AFJ19235.1| mixed amyrin synthase [Catharanthus roseus]
          Length = 762

 Score =  518 bits (1333), Expect = e-145
 Identities = 239/304 (78%), Positives = 276/304 (90%)
 Frame = +3

Query: 3    IQLRQEIYPIPYDDINWNKQRHNCCKEDLYYPHSFIQDLLWDGLHYLGEPIINSWPFNKI 182
            +QLRQEI+  PYD I+WNK RH+CCK+DLYYPHSFIQDLLWD L+Y  EP++  WP NKI
Sbjct: 285  MQLRQEIHVKPYDQIDWNKARHDCCKDDLYYPHSFIQDLLWDTLNYFSEPVMRRWPCNKI 344

Query: 183  RKRGLKRAIELMRYGAEESRYITIGCVEKSLQMMCWWAENPDGDEFKHHLARVPDYLWLA 362
            R++ +++ I+ MRYGAEESRYITIGCVEKSLQMMCW A +P+ DEFK+HLARVPDYLWLA
Sbjct: 345  REKAMRKCIKYMRYGAEESRYITIGCVEKSLQMMCWCAHDPNCDEFKYHLARVPDYLWLA 404

Query: 363  EDGMKMQSFGSQLWDCVLATQAIMATGMVEEYGDSLKKAHFYIKESQVKDNPSGDFESMC 542
            EDGMKMQSFGSQLWDC LATQAI+ATGMVEEYGD++KKAHFYIKESQVK+NP  DF++M 
Sbjct: 405  EDGMKMQSFGSQLWDCTLATQAIIATGMVEEYGDTIKKAHFYIKESQVKENPKEDFKAMY 464

Query: 543  RKFTKGSWTFSDQDQGWVVSDCTAEALKCLLSLSQMPEEIAGEKAQVERLHEAVDVLLYL 722
            R FTKGSWTFSDQDQGWVVSDCTAEALKCLL  SQMP+++AGEKA VERL++AV+VLLYL
Sbjct: 465  RHFTKGSWTFSDQDQGWVVSDCTAEALKCLLVCSQMPQDLAGEKADVERLYDAVNVLLYL 524

Query: 723  QSPETGGFAIWEPPVPQPYLQILNPSELFADIVVEKEHLECTASIMEALLAFKHLHHGYR 902
            QSPE+GGFAIWEPPVPQPYLQ+LNPSELFADIVVE+EH+E TASI++AL+ FK LH G+R
Sbjct: 525  QSPESGGFAIWEPPVPQPYLQVLNPSELFADIVVEQEHVENTASIVQALVLFKRLHPGHR 584

Query: 903  EKEI 914
            EKEI
Sbjct: 585  EKEI 588


>gb|AEX99665.1| amyrin synthase [Catharanthus roseus]
          Length = 762

 Score =  518 bits (1333), Expect = e-145
 Identities = 239/304 (78%), Positives = 276/304 (90%)
 Frame = +3

Query: 3    IQLRQEIYPIPYDDINWNKQRHNCCKEDLYYPHSFIQDLLWDGLHYLGEPIINSWPFNKI 182
            +QLRQEI+  PYD I+WNK RH+CCK+DLYYPHSFIQDLLWD L+Y  EP++  WP NKI
Sbjct: 285  MQLRQEIHVKPYDQIDWNKARHDCCKDDLYYPHSFIQDLLWDTLNYFSEPVMRRWPCNKI 344

Query: 183  RKRGLKRAIELMRYGAEESRYITIGCVEKSLQMMCWWAENPDGDEFKHHLARVPDYLWLA 362
            R++ +++ I+ MRYGAEESRYITIGCVEKSLQMMCW A +P+ DEFK+HLARVPDYLWLA
Sbjct: 345  REKAMRKCIKYMRYGAEESRYITIGCVEKSLQMMCWCAHDPNCDEFKYHLARVPDYLWLA 404

Query: 363  EDGMKMQSFGSQLWDCVLATQAIMATGMVEEYGDSLKKAHFYIKESQVKDNPSGDFESMC 542
            EDGMKMQSFGSQLWDC LATQAI+ATGMVEEYGD++KKAHFYIKESQVK+NP  DF++M 
Sbjct: 405  EDGMKMQSFGSQLWDCTLATQAIIATGMVEEYGDTIKKAHFYIKESQVKENPKEDFKAMY 464

Query: 543  RKFTKGSWTFSDQDQGWVVSDCTAEALKCLLSLSQMPEEIAGEKAQVERLHEAVDVLLYL 722
            R FTKGSWTFSDQDQGWVVSDCTAEALKCLL  SQMP+++AGEKA VERL++AV+VLLYL
Sbjct: 465  RHFTKGSWTFSDQDQGWVVSDCTAEALKCLLVCSQMPQDLAGEKADVERLYDAVNVLLYL 524

Query: 723  QSPETGGFAIWEPPVPQPYLQILNPSELFADIVVEKEHLECTASIMEALLAFKHLHHGYR 902
            QSPE+GGFAIWEPPVPQPYLQ+LNPSELFADIVVE+EH+E TASI++AL+ FK LH G+R
Sbjct: 525  QSPESGGFAIWEPPVPQPYLQVLNPSELFADIVVEQEHVENTASIVQALVLFKRLHPGHR 584

Query: 903  EKEI 914
            EKEI
Sbjct: 585  EKEI 588