BLASTX nr result
ID: Bupleurum21_contig00005301
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00005301
(2539 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAN76961.1| hypothetical protein VITISV_029501 [Vitis vinifera] 346 2e-92
emb|CBI22670.3| unnamed protein product [Vitis vinifera] 252 4e-64
ref|XP_002327472.1| predicted protein [Populus trichocarpa] gi|2... 222 3e-55
ref|XP_002305913.1| predicted protein [Populus trichocarpa] gi|2... 207 1e-50
emb|CBI34371.3| unnamed protein product [Vitis vinifera] 133 3e-28
>emb|CAN76961.1| hypothetical protein VITISV_029501 [Vitis vinifera]
Length = 1362
Score = 346 bits (888), Expect = 2e-92
Identities = 265/794 (33%), Positives = 373/794 (46%), Gaps = 109/794 (13%)
Frame = +3
Query: 111 HPSPKGEALSASSTSVVVGE------VKLLADKASAADNNVDDVPQHNGPMGLFNNSN-- 266
H SP+ + +SAS S + G+ VK D+ SA D D H G L N S+
Sbjct: 571 HSSPRCDDVSASGASAIEGQSVVTVGVKEHNDQVSADDATEVDPSVHVGARELVNKSSEH 630
Query: 267 ------------------------GSPSKVDQDNNISDCYDSNATKDCK--KVNMDHISE 368
KVD ++ D YD++ ++D + V M++++E
Sbjct: 631 STISGEQSEFIPDEVGKNCDDDPANCSGKVDLEDPFDDSYDTDVSQDDRGHPVGMENVTE 690
Query: 369 YEAGYDSSFEDGELREPGVYTWEDNDLEG----------------DTEYVDY-------- 476
+AGYDS EDGELRE V+ WE+ND E D + VDY
Sbjct: 691 LDAGYDSQIEDGELRESVVHAWEENDAEDGEAERVDYESDNRDMYDFDAVDYPGPMTGEV 750
Query: 477 --GSE------YGDGDDFDTVNSKSGNAENGQVFYQSC-------------------TKG 575
GSE G F + N G + QSC +K
Sbjct: 751 EVGSECEKERLLGPNHHFGCGETTINNGVKG-ISDQSCLGGSLANEAEFSNGGLVKTSKP 809
Query: 576 VSVXXXXXXXXXXXXXXRSAVPGDSVKSLKQCFNGDAQEGSPSACMKTNIFGKDKFNEHL 755
S S D + ++Q G G+ +TN+ D+
Sbjct: 810 QSWTQFTRKVDTNIKRGSSTGTNDVAEEVEQPAGGG---GALKEQSQTNVAQYDQLPNDR 866
Query: 756 EYRVADEKVGNYDDKGLYGEYGSRAFRGKLPSFSKGHSPFDAVDRRTLGIHQKRFDNNY- 932
E D+ V D + + G R+ R +L S +G S +D + R+ I Q+ NN
Sbjct: 867 EIST-DKNVEVNDGRAI----GPRSTRRELLSRIEGPS-YDILRRKDAVILQRSRSNNLD 920
Query: 933 -SYTRAESDFSLEKSMGRGRFPIKPFGSRNETGGRWVDSPSSYRDTRNRYPQSYRGAESH 1109
S RAE +KSMGR R + G R + G W + Y D++ R+ SY
Sbjct: 921 DSDPRAERGTDSDKSMGRSRSALHIHG-RGQRDGNWDQPSTGYWDSKRRHSPSYHAPYGS 979
Query: 1110 AYSRPRD---------SPAKSVTK--IAGFNSEDRRSINYSSHNGSYRSFMGRRSPVDRE 1256
RPR + ++++K + G N R+S+N SS G YR + RRSP DR+
Sbjct: 980 GRPRPRSIVETGGFVMTSDRTISKAGVGGLNGIHRQSMN-SSSKGVYRPLIRRRSPSDRD 1038
Query: 1257 DSYFGQRGLPPARGIHQDRSRSTSTRYVQGIRRGTKDEYTEELPDDTSLHSMRRQSYFPR 1436
D+Y G+ P R + +RSR S RY QG+ RG ++ Y +PD+T +R + R
Sbjct: 1039 DAYGMHMGMAPGRDVSPERSRGRSRRY-QGVHRGPRENYHGSIPDETXEFPLRVPHHLAR 1097
Query: 1437 RQQGFSP--NYGGGQFLXXXXXXXXXXXXXXPVVWNPQRERNINTRR--RSPDFRSDARM 1604
R++ SP N G F P W RERN ++R RSPDFRS ARM
Sbjct: 1098 RERSISPIFNRGAPHFSETHKISQSRSRSRSPPAWLMSRERNASSRHFSRSPDFRSGARM 1157
Query: 1605 ERVRSPFQKTNFGTNNEESYMSPPRGRVSPRHNSRWVTEGNYMDNHLRD-RSPVRMFRRS 1781
ER+R PFQK NF + EE ++SPPRGR+SP+HNSRW+ + N +H RD RSPVRMF++S
Sbjct: 1158 ERMRLPFQKPNFADDYEEGFLSPPRGRISPQHNSRWIDDRNGAMDHFRDGRSPVRMFQQS 1217
Query: 1782 QRLDPIGYSGR--KSDGIFQPNIRRGRYQQVSSSTRGSDFEGSDDDKRKHDDRYEINHRV 1955
QR D +G R +D +P I GR ++ + RG + SDDD+RKH DRYE+ V
Sbjct: 1218 QRFDSMGPPRRLKSNDYYSRPMIHPGRLPEMVGAGRGRKHDDSDDDRRKHGDRYEMIRPV 1277
Query: 1956 RRYDAGGAVRRFRYDADDCFDARNAHKDDSFGRAI---KRDNPRNGAEENRGPKYDNDRM 2126
RRYD GG V+RFRYD +DC +RN H +D R RD PR +EE R +Y+++R+
Sbjct: 1278 RRYDTGGVVKRFRYDTEDCVLSRNPHNNDDCIRGTDRRPRDIPRRPSEEKRHLRYNHERL 1337
Query: 2127 Y-ASASSVKAREFN 2165
Y +S +S RE++
Sbjct: 1338 YNSSPNSFGMREYD 1351
>emb|CBI22670.3| unnamed protein product [Vitis vinifera]
Length = 1191
Score = 252 bits (643), Expect = 4e-64
Identities = 209/664 (31%), Positives = 294/664 (44%), Gaps = 99/664 (14%)
Frame = +3
Query: 111 HPSPKGEALSASSTSVVVGE------VKLLADKASAADNNVDDVPQHNGPMGLFNNSN-- 266
H SP+ + +SAS S + G+ VK D+ SA D D H G L N S+
Sbjct: 535 HSSPRCDDVSASGASAIEGQSVVTVGVKEHNDQVSADDATEVDPSVHVGARELVNKSSEH 594
Query: 267 ------------------------GSPSKVDQDNNISDCYDSNATKDCK-KVNMDHISEY 371
KVD ++ D YD++ ++D + M++++E
Sbjct: 595 STISGERSEFIPDEVGKNCDDDPANCSGKVDLEDPFDDSYDTDVSQDDRGHPGMENVTEL 654
Query: 372 EAGYDSSFEDGELREPGVYTWEDNDLEG----------------DTEYVDY--------- 476
+AGYDS EDGELRE V+ WE+ND E D + VDY
Sbjct: 655 DAGYDSQIEDGELRESVVHAWEENDAEDGEAERVDYESDNRDMYDFDAVDYPGPMTGEVE 714
Query: 477 -GSE------YGDGDDFDTVNSKSGNAENGQVFYQSCTKG-------------VSVXXXX 596
GSE G F + N G + QSC G V
Sbjct: 715 VGSECEKERLLGPNHHFGCGETTIDNGVKG-ISDQSCLGGSLANEAEFSNGGLVKTSKPQ 773
Query: 597 XXXXXXXXXXRSAVPGDSVKSLKQCFNGDAQEGSPSACMK---TNIFGKDKFNEHLEYRV 767
+ G S + + G A + TN+ D+ E
Sbjct: 774 SWTQFTRKVDTNIKRGSSTGTNDVAEEAEQPAGGGGALKEQSQTNVAQYDQLPNDREIST 833
Query: 768 ADEKVGNYDDKGLYGEYGSRAFRGKLPSFSKGHSPFDAVDRRTLGIHQKRFDNNY--SYT 941
D+ V D + + G R+ R +L S +G S +D + R+ I Q+ NN S
Sbjct: 834 -DKNVEVNDGRAI----GPRSTRRELLSRIEGPS-YDILHRKDAVILQRSRSNNLDDSDP 887
Query: 942 RAESDFSLEKSMGRGRFPIKPFGSRNETGGRWVDSPSSYRDTRNRYPQSYRGAESHAYSR 1121
RAE +KSMGR R + G R + G W + Y D++ R+ SY R
Sbjct: 888 RAERGTDSDKSMGRSRSALHIHG-RGQRDGNWDQPSTGYWDSKRRHSPSYHAPYGSGRPR 946
Query: 1122 PRD---------SPAKSVTK--IAGFNSEDRRSINYSSHNGSYRSFMGRRSPVDREDSYF 1268
PR + ++++K + G N R+S+N SS G YR + RRSP DR+D+Y
Sbjct: 947 PRSIVETGGFVMTSDRTISKAGVGGLNGIHRQSMN-SSSKGVYRPLIRRRSPSDRDDAYG 1005
Query: 1269 GQRGLPPARGIHQDRSRSTSTRYVQGIRRGTKDEYTEELPDDTSLHSMRRQSYFPRRQQG 1448
G+ P R + +RSR S RY QG+ RG ++ Y +PD+T +R + RR++
Sbjct: 1006 MHMGMAPGRDVSPERSRGRSRRY-QGVHRGPRENYHGSIPDETDEFPLRVPHHLARRERS 1064
Query: 1449 FSP--NYGGGQFLXXXXXXXXXXXXXXPVVWNPQRERNINTRR--RSPDFRSDARMERVR 1616
SP N G F P W RERN ++R RSPDFRS ARMER+R
Sbjct: 1065 ISPIFNRGAPHFSETHKISQSRSRSRSPPAWLMSRERNASSRHFSRSPDFRSGARMERMR 1124
Query: 1617 SPFQKTNFGTNNEESYMSPPRGRVSPRHNSRWVTEGNYMDNHLRD-RSPVRMFRRSQRLD 1793
PFQK NF + EE ++SPPRGR+SP+HNSRW+ + N +H RD RSPVRMF++SQ L
Sbjct: 1125 LPFQKPNFADDYEEGFLSPPRGRISPQHNSRWIDDRNGAMDHFRDGRSPVRMFQQSQLLL 1184
Query: 1794 PIGY 1805
+G+
Sbjct: 1185 GLGF 1188
>ref|XP_002327472.1| predicted protein [Populus trichocarpa] gi|222836026|gb|EEE74447.1|
predicted protein [Populus trichocarpa]
Length = 1218
Score = 222 bits (566), Expect = 3e-55
Identities = 210/687 (30%), Positives = 297/687 (43%), Gaps = 24/687 (3%)
Frame = +3
Query: 87 DTIGASHFHPSPKGEALSASSTSVVVGEVKLLADKASAADNNV---DDVPQHNGPMGLFN 257
D G + + K EA S V G +L+ + S+AD+ V D H N
Sbjct: 590 DANGTNEASAANKAEAHSP----VQAGSEELM--QKSSADSTVTPGDACGTHG------N 637
Query: 258 NSNGSPSKVDQDNNISDCYDSNATKDCKKVNMDHIS--EYEAGYDSSFEDGELREPGV-Y 428
+KV+ ++ D ++S+ + KV++ I+ E +AGYDS FEDGELRE Y
Sbjct: 638 GFTSGSAKVNMEDLEDDSFESDVYQ-ADKVHIVGINGLELQAGYDSPFEDGELRESDAQY 696
Query: 429 TWEDNDLEGDTEYVDYGSEYGDGDDFDTVNSKSGNAENGQVFYQSCTKGVSVXXXXXXXX 608
W++N +G+ E VDYGSE + N K E G
Sbjct: 697 YWDENGEDGEVEQVDYGSECDEERLCVLDNEKEMKVERG--------------------- 735
Query: 609 XXXXXXRSAVPGDSV-KSLKQCFNGDAQEGSPSACMKTNIFGKDKFNEHLEYRVADEKVG 785
S+ D V + ++QC GD+ ++ ++ ++
Sbjct: 736 -------SSSGSDYVSRKIEQCGLGDS--------LRDDLLSPKTRTSYV---------- 770
Query: 786 NYDDKGLYGEYGSRAF-RGKLPSFSKGHSPFDAVDRRTLGIHQKRFDNNYSYTRAESDFS 962
D L G GS+A R L S + ++ F RTL F N Y R E D
Sbjct: 771 TIDKDFLSGVVGSKASDRDFLSSIEESNAIFRK--HRTLRSRSNSFYNLYH--RDERDAG 826
Query: 963 LEKSMGRGRFPIKPFGSRNETGGRWVDSPSSYRDTRNRYPQSYRGA------------ES 1106
+K MGR R + P R+ G R+V+ Y D+ R+ +YRG +S
Sbjct: 827 SQKFMGRDR-AVPPTRGRSPGGHRFVNDAPGYCDSERRHFCTYRGNYTSGPSRTRGGFDS 885
Query: 1107 HAYSRPRDSPAKSVTKIAGFNSEDRRSINYSSHNGSYRSFMGRRSPVDREDSYFGQRGLP 1286
H Y D A AG +S RR S N SY RRSP R+D Y G
Sbjct: 886 HRYVITSDRAASEGAGFAGSDSRGRRRFVNPSSNSSYERVTRRRSPTSRDDLYRVHTGTQ 945
Query: 1287 PARGIHQDRSRSTSTRYVQGIRRG-TKDEYTEELPDDTSLHSMRRQSYFPRRQQGFSPNY 1463
P R RS R+ +G G + EY +P+D +S R RR++ SP
Sbjct: 946 PVRD--GSPVRSGFRRFPRGAATGGLRGEYHRPMPEDKIEYSNRYAPRMLRRERSISPLC 1003
Query: 1464 GGG-QFLXXXXXXXXXXXXXXPVVWNPQRERNINTRRRSPDFRSDARMERVRSPFQKTNF 1640
G + P R R RSPDFR+DARM+RVR PFQK
Sbjct: 1004 RGQPSYPFTHKKSRSRSRSRSPSYLTRDRNEASRLRSRSPDFRTDARMDRVRLPFQK-RI 1062
Query: 1641 GTNNEESYMSPPRGRVSPRHNSRWVTEGNY-MDNHLRDRSPVRMFRRSQRLDPIGYSGR- 1814
+ EE ++ P R +HN RW + N +D+ +SPV MFR QR D + R
Sbjct: 1063 PADFEEGFI-PTRRNHFTQHNPRWFDDRNGGLDSFRGRKSPVNMFRSDQRFDSVRTIRRL 1121
Query: 1815 KSDGIFQPNIRRGRYQQVSSSTRGSDFEGSDDDKRKHDDRYEINHRVRRYDAGGAVRRFR 1994
S+ F+ IR ++ + S++RG +F+ SDD +RKH+++YEI RVRRYD G +RRFR
Sbjct: 1122 DSEDQFRERIRSRKFNDMGSASRGGEFDSSDDGRRKHNNKYEIVQRVRRYDTDGGLRRFR 1181
Query: 1995 YDADDCFDARNAHKDDSFGRAIKRDNP 2075
+A+D A NA + G I P
Sbjct: 1182 VNAEDSLVANNATPNCDDGNRITDRRP 1208
>ref|XP_002305913.1| predicted protein [Populus trichocarpa] gi|222848877|gb|EEE86424.1|
predicted protein [Populus trichocarpa]
Length = 1290
Score = 207 bits (526), Expect = 1e-50
Identities = 204/693 (29%), Positives = 300/693 (43%), Gaps = 21/693 (3%)
Frame = +3
Query: 87 DTIGASHFHPSPKGEALSASSTSVVVGEVKLLADKASAADNNVDDVPQHNGPMGLFNNSN 266
D GA+ + K E S V G +L+ + S+AD+ V G N
Sbjct: 665 DARGANEASAANKAEVHSP----VQAGSEELM--QKSSADSTVTP----GDACGAHGNGL 714
Query: 267 GSPSKVDQDNNISDCYDSNATKDCKK-VNMDHISEYEAGYDSSFEDGELREPGVYT-WED 440
S S ++ D ++S+ + K V ++ E +AGYDS FEDGELRE W++
Sbjct: 715 TSVSANVNMEDLEDSFESDVYQADKVIVGTENDLELQAGYDSQFEDGELRESDARLYWDE 774
Query: 441 NDLEGDTEYVDYGSEYGDGDDFDTVNSKSGNAENGQVFYQSCTKGVSVXXXXXXXXXXXX 620
N +G+ E+VDYGSE + N K E G
Sbjct: 775 NGEDGEVEHVDYGSECDEERLCGMDNEKEMKVERGS------------------------ 810
Query: 621 XXRSAVPGDSVKSLKQCFNGDAQEGSPSACMKTNIFGKDKFNEHLEYRVADEKVGNYDDK 800
S+ D+ + ++ ++ S S +T+ DK
Sbjct: 811 ---SSGSDDASRKIEHGMGDSLRDDSVSPKTRTSDVTTDK-------------------D 848
Query: 801 GLYGEYGSRAF-RGKLPSFSKGHSPFDAVDRRTLGIHQKRFDNNYS-YTRAESDFSLEKS 974
L G GSR R L S + S F R + R N Y+ Y R E D K
Sbjct: 849 FLSGVVGSRTSNRDFLSSIEESSSIF-----RKDPTLRSRAGNIYNLYPRDERDAGSHKF 903
Query: 975 MGRGRFPIKPFGSRNETGGRWVDSPSSYRDTRNRYPQSYRGAESHAYSRPR--------- 1127
MGR R + R+ G R+V+ + Y D+ RY +YRG + + R R
Sbjct: 904 MGRDR-AVPQMRGRSPGGHRFVNHATGYCDSERRYLSNYRGNYTSGHPRTRGGFDSRRYI 962
Query: 1128 ---DSPAKSVTKIAGFNSEDRRSINYSSHNGSYRSFMGRRSPVDREDSYFGQRGLPPARG 1298
D AG ++ RR S G+Y + RRSP R+DSY G P R
Sbjct: 963 ISSDHTDSEGVGFAGSDNRARRRFVNPSSTGAYERIVRRRSPTSRDDSYRVHTGALPVRD 1022
Query: 1299 IHQDRSRSTSTRYVQGIRRG-TKDEYTEELPDDTSLHSMRRQSYFPRRQQGFSPNYGGG- 1472
RS R+ + + RG ++EY +P+D +S R RR++ SP G
Sbjct: 1023 --GSPIRSGFRRFPREVARGGLREEYHRPMPEDKIEYSNRFAPRMLRRERSISPLCRGQP 1080
Query: 1473 QFLXXXXXXXXXXXXXXPVVWNPQRERNINT-RRRSPDFRSDARMERVRSPFQKTNFGTN 1649
+ P + P+ ++ R RSPDFR+DARM+RVR PFQK F +
Sbjct: 1081 RHPFTHKKSRSRSRSRSPSSYLPRVRNEVSRLRSRSPDFRTDARMDRVRLPFQK-RFPAD 1139
Query: 1650 NEESYMSPPRGRVSPRHNSRWVTEGNY-MDNHLRDRSPVRMFRRSQRLDPIGYSGR-KSD 1823
E ++ P R +HN RW + N +D+ +SPV MFR +QR D + R S+
Sbjct: 1140 FEGDFI-PTRRNHFTQHNPRWFDDRNGGLDSFRGRKSPVNMFRPNQRFDSVRTIRRLDSE 1198
Query: 1824 GIFQPNIRRGRYQQVSSSTRGSDFEGSDDDKRKHDDRYEINHRVRRYDAGGAVRRFRYDA 2003
F+P +R ++ + S++RG +F+GSDDD+RKH +RY++ RVR+YD ++RRFR++A
Sbjct: 1199 DQFRPMMRSRKFNDMGSASRGGEFDGSDDDRRKH-NRYDMAQRVRQYDT-DSLRRFRFNA 1256
Query: 2004 DDCFDARNAHKDDSFGRAIKRDNPRNGAEENRG 2102
+D A N + G I P GA G
Sbjct: 1257 EDSLVANNDTPNCDEGNRITDRRP-GGAHRRDG 1288
>emb|CBI34371.3| unnamed protein product [Vitis vinifera]
Length = 1242
Score = 133 bits (334), Expect = 3e-28
Identities = 147/567 (25%), Positives = 219/567 (38%), Gaps = 87/567 (15%)
Frame = +3
Query: 312 YDSNATKDCKK--VNMDHISEYEAGYDSSFEDGELREPGVYTWED-NDLEGDTEYVDYGS 482
YDS+ ++D V ++ SE + YDS +EDGELRE YTWED +G+ E+VDYGS
Sbjct: 689 YDSDVSQDDPDPVVRIEKASELQMDYDSQYEDGELRESIEYTWEDLGGEDGEGEHVDYGS 748
Query: 483 EYGD-----GDDFDTVNSK-----------SGNAENGQVFYQSCTKGVSVXXXXXXXXXX 614
+ D D+ + K S N G QSC S+
Sbjct: 749 DNRDMAGFGASDYHVMLVKVEGAECKRQRVSNNKNLGSEPEQSCLGSSSMTKVVEAVSGK 808
Query: 615 XXXXRSAVP----------------------------GDSVKSLKQC--FNGDAQEGSPS 704
+ + P D+V + DA+E S
Sbjct: 809 DDGAKCSSPCLSTRFSGKDDIDQFDASAEVNKETGARPDNVSGDHEIHVMEADAEEASQD 868
Query: 705 ACMKTNIFGKDKFNEHLEYR--VADEKVGNYDDKGLYGE--------------YGSRAFR 836
+K I G D E+ + +A E K + G+ SR F+
Sbjct: 869 DDLKMKISGWDLLPENCKSSSDIAMELGDGSGRKNISGDSVDGLNTEDTETRMVKSRTFK 928
Query: 837 GKLPSFSKGHSPFDAVDRRTLGIHQ------KRFDNNYSYTRAESDFSLEKSMGRGRFPI 998
+L S +G D LG H+ + D + S +R+E + KS GR + +
Sbjct: 929 RELLSRIEGPVSGDVF----LGKHRLCTQGSRSNDADDSNSRSERESGSVKSFGRSSYSL 984
Query: 999 KPFGSRNETGGRWVDSPSSYRDTRNRYPQSYRGAESHAYSRPRDSPAKSVTKIAGFNSED 1178
SR W D P +R R + YR ++ P ++ A +
Sbjct: 985 H-IHSRGRGSEHWADYPDGHRAPRRHHSSGYRSITGFSFPGPENAAAVFTS--------- 1034
Query: 1179 RRSINYSSHNGSYRSFMGRRSPVDREDSYFGQRGLPPARGIHQDR----SRSTSTRYVQG 1346
R+S + SSH G R+ SP + ++++ + GL PA I DR R S RY
Sbjct: 1035 RQSTSVSSH-GVRRALRRSGSPAEGDEAFGMRLGLRPAGEISPDRHMGIGRERSLRYGPR 1093
Query: 1347 IR-RGTKDEYTEELPDDTSLHSMRRQSYFPRRQQGFSP--NYGGGQFLXXXXXXXXXXXX 1517
+ RG + Y + D S+ +R++ FSP G
Sbjct: 1094 LDDRGPRGRYDGHVLDGCIESSLNYSHPLAKRERSFSPIERRGDPSARQSHTKSPSRSRS 1153
Query: 1518 XXPVVWNPQRERN---------INTRRRSPDFRSDARMERVRSPFQKTNFGTNNEESYMS 1670
P W R RN + R RSP FRS AR++R+RSP Q++ F +++ +MS
Sbjct: 1154 RSPNTWQSPRGRNGAGVVGNPCLRHRSRSPSFRSGARVQRIRSPHQRSGFASDHMVGFMS 1213
Query: 1671 PPRGRVSPRHNSRWVTEGNYMDNHLRD 1751
PR SP HNSRW+ + H R+
Sbjct: 1214 MPRNHGSPPHNSRWIGDRKDGMVHFRE 1240