BLASTX nr result
ID: Bupleurum21_contig00005270
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00005270
(2300 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003632552.1| PREDICTED: probable ADP-ribosylation factor ... 314 6e-83
emb|CBI31191.3| unnamed protein product [Vitis vinifera] 314 6e-83
gb|ADN33979.1| GTPase activating protein [Cucumis melo subsp. melo] 307 7e-81
ref|XP_004136610.1| PREDICTED: probable ADP-ribosylation factor ... 305 5e-80
ref|XP_002530953.1| HIV-1 rev binding protein, hrbl, putative [R... 303 2e-79
>ref|XP_003632552.1| PREDICTED: probable ADP-ribosylation factor GTPase-activating protein
AGD14-like [Vitis vinifera]
Length = 677
Score = 314 bits (805), Expect = 6e-83
Identities = 192/390 (49%), Positives = 229/390 (58%), Gaps = 18/390 (4%)
Frame = -2
Query: 2296 RKAEERNEKIIRGLMKLPPNRRCINCNALGPQYVCTDFWTFVCMICSGIHREFTHRVKSV 2117
RK EERNEKIIRGLMKLPPNRRCINCN+LGPQYVCT+FWTFVC CSGIHREFTHRVKSV
Sbjct: 4 RKEEERNEKIIRGLMKLPPNRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHRVKSV 63
Query: 2116 SMSKFTAQEVEALERGGNQRARETFLHDWDPQRHRFPVSSNADKVREFIKNVYVDRKYAS 1937
SM+KFT+QEVEAL++GGNQRARE +L DWD QR R P SN DKVR+FIK+VYVDR+YA
Sbjct: 64 SMAKFTSQEVEALQKGGNQRARELYLKDWDTQRQRLPDISNVDKVRDFIKSVYVDRRYAV 123
Query: 1936 KKTCEKPPRNLQDHKTHEDLTRRAXXXXXXXXXXXXXXXYEERRYGKQAFALTRKAGSDR 1757
KT +KPPR+ Q+ + ++ TRRA YE+RRYGKQA LTRK GSDR
Sbjct: 124 GKTSDKPPRDTQNLRDRDEETRRASSYHSYSQSPPYDYQYEDRRYGKQAAVLTRKPGSDR 183
Query: 1756 GLK----TSSSFSPGRLGDYDIEEKFKKGHXXXXXXXXXXXSAGDPCRSYNQSPTTERGI 1589
GL +S +SPGRL D E++F +GDP RS QSP ++GI
Sbjct: 184 GLYDGKISSFVYSPGRLSDQMYEDRFAN------------DGSGDPFRSNIQSPNFQKGI 231
Query: 1588 KVSSPPSETCGDMLSLDEWNQKVNK-FSDNSKVYEDRSPHLKRTAS-------NSNSMPF 1433
SSPP D+L D Q +N +NS+ D +RT S +SNSM
Sbjct: 232 GFSSPPIHPSRDILIGDAQQQTINTVVEENSR--RDGFQRPQRTTSSGSFGSFDSNSMSI 289
Query: 1432 SSVNSVK----ASGAEQAT-GTHCKLTTXXXXXXXXXXSNY-IGLDLFDTMFASQVXXXX 1271
S NS S EQ+T G H + S Y + LDLFD FA +
Sbjct: 290 KSANSSNLMDVVSEPEQSTGGAHQDKISELHALPRSSVSGYPVSLDLFDAPFAPET---- 345
Query: 1270 XXXXXXXXXXXSGLFKSPEISSASPIDMFQ 1181
LF+ P SS +D+FQ
Sbjct: 346 ----VTSSASSIDLFQLPVTSSVPSVDLFQ 371
Score = 64.7 bits (156), Expect(2) = 1e-12
Identities = 56/218 (25%), Positives = 85/218 (38%), Gaps = 67/218 (30%)
Frame = -3
Query: 807 DQKRSVATFNTTSSDIVNQQYGGWATFDGLQNMEPSQGG-QSSTSAAIPSSEGSSASDVD 631
+Q++S AT + ++ + GWATFD M G ++ T A IPS++G +D
Sbjct: 402 NQQQSAATLHQKLPELSVPKNEGWATFDTPTPMTSIAGTTENCTPAKIPSTDGGPPGKLD 461
Query: 630 QYLYRNTTQQWSAFHDSSAQVTYPSMP--------------------------------- 550
L + + QW F DS+A SM
Sbjct: 462 SVLSIDASMQWPLFQDSNAHGPSSSMSNPWDQGLHNVQASANVTSTPLWNAFEDAIGHLS 521
Query: 549 ---IQWQNSPHNNVGIPSKSYQ-----------------------------AEALSSTTN 466
IQ + PH V PS + Q T
Sbjct: 522 LGSIQQSSEPHVAVSKPSTADQFLGSRVLEDPDNDGIQRAASAGGPSLPSGGTQSQGTDK 581
Query: 465 RSTNPFDIAYEYDVAPNNVFLDMSSMQGALP-NSEMPT 355
+S+NPFD+ Y+ D+ +++FLDMSS+Q ALP N ++P+
Sbjct: 582 KSSNPFDLPYDSDLEQSSMFLDMSSLQAALPSNPQLPS 619
Score = 36.6 bits (83), Expect(2) = 1e-12
Identities = 15/23 (65%), Positives = 20/23 (86%)
Frame = -2
Query: 235 PSAQLASIQSEGPVAPVGGNPFA 167
PS+QL + Q++GPVA +GGNPFA
Sbjct: 655 PSSQLPNGQTQGPVASIGGNPFA 677
>emb|CBI31191.3| unnamed protein product [Vitis vinifera]
Length = 695
Score = 314 bits (805), Expect = 6e-83
Identities = 192/390 (49%), Positives = 229/390 (58%), Gaps = 18/390 (4%)
Frame = -2
Query: 2296 RKAEERNEKIIRGLMKLPPNRRCINCNALGPQYVCTDFWTFVCMICSGIHREFTHRVKSV 2117
RK EERNEKIIRGLMKLPPNRRCINCN+LGPQYVCT+FWTFVC CSGIHREFTHRVKSV
Sbjct: 4 RKEEERNEKIIRGLMKLPPNRRCINCNSLGPQYVCTNFWTFVCTTCSGIHREFTHRVKSV 63
Query: 2116 SMSKFTAQEVEALERGGNQRARETFLHDWDPQRHRFPVSSNADKVREFIKNVYVDRKYAS 1937
SM+KFT+QEVEAL++GGNQRARE +L DWD QR R P SN DKVR+FIK+VYVDR+YA
Sbjct: 64 SMAKFTSQEVEALQKGGNQRARELYLKDWDTQRQRLPDISNVDKVRDFIKSVYVDRRYAV 123
Query: 1936 KKTCEKPPRNLQDHKTHEDLTRRAXXXXXXXXXXXXXXXYEERRYGKQAFALTRKAGSDR 1757
KT +KPPR+ Q+ + ++ TRRA YE+RRYGKQA LTRK GSDR
Sbjct: 124 GKTSDKPPRDTQNLRDRDEETRRASSYHSYSQSPPYDYQYEDRRYGKQAAVLTRKPGSDR 183
Query: 1756 GLK----TSSSFSPGRLGDYDIEEKFKKGHXXXXXXXXXXXSAGDPCRSYNQSPTTERGI 1589
GL +S +SPGRL D E++F +GDP RS QSP ++GI
Sbjct: 184 GLYDGKISSFVYSPGRLSDQMYEDRFAN------------DGSGDPFRSNIQSPNFQKGI 231
Query: 1588 KVSSPPSETCGDMLSLDEWNQKVNK-FSDNSKVYEDRSPHLKRTAS-------NSNSMPF 1433
SSPP D+L D Q +N +NS+ D +RT S +SNSM
Sbjct: 232 GFSSPPIHPSRDILIGDAQQQTINTVVEENSR--RDGFQRPQRTTSSGSFGSFDSNSMSI 289
Query: 1432 SSVNSVK----ASGAEQAT-GTHCKLTTXXXXXXXXXXSNY-IGLDLFDTMFASQVXXXX 1271
S NS S EQ+T G H + S Y + LDLFD FA +
Sbjct: 290 KSANSSNLMDVVSEPEQSTGGAHQDKISELHALPRSSVSGYPVSLDLFDAPFAPET---- 345
Query: 1270 XXXXXXXXXXXSGLFKSPEISSASPIDMFQ 1181
LF+ P SS +D+FQ
Sbjct: 346 ----VTSSASSIDLFQLPVTSSVPSVDLFQ 371
Score = 51.6 bits (122), Expect(2) = 9e-09
Identities = 54/238 (22%), Positives = 86/238 (36%), Gaps = 87/238 (36%)
Frame = -3
Query: 807 DQKRSVATFNTTSSDIVNQQYGGWATFDGLQNMEPSQGG-QSSTSAAIPSSEGSSASDVD 631
+Q++S AT + ++ + GWATFD M G ++ T A IPS++G +D
Sbjct: 402 NQQQSAATLHQKLPELSVPKNEGWATFDTPTPMTSIAGTTENCTPAKIPSTDGGPPGKLD 461
Query: 630 QYLYRNTTQQ--------------------------------------WSAFHDSSAQVT 565
L + + Q W+AF D+ ++
Sbjct: 462 SVLSIDASMQWPLFQDSNAHGPSSSMSNPWDQGLHNVQASANVTSTPLWNAFEDAIGHLS 521
Query: 564 YPSMPIQWQNSPHNNVGIPSKSYQ------------------------------------ 493
S IQ + PH V PS + Q
Sbjct: 522 LGS--IQQSSEPHVAVSKPSTADQFLGSRVLEDPDNDGIQRAASAGGPSLPSVPTHVTGP 579
Query: 492 -----------AEALSSTTNRSTNPFDIAYEYDVAPNNVFLDMSSMQGALP-NSEMPT 355
T +S+NPFD+ Y+ D+ +++FLDMSS+Q ALP N ++P+
Sbjct: 580 PDAPSELALMGGTQSQGTDKKSSNPFDLPYDSDLEQSSMFLDMSSLQAALPSNPQLPS 637
Score = 36.6 bits (83), Expect(2) = 9e-09
Identities = 15/23 (65%), Positives = 20/23 (86%)
Frame = -2
Query: 235 PSAQLASIQSEGPVAPVGGNPFA 167
PS+QL + Q++GPVA +GGNPFA
Sbjct: 673 PSSQLPNGQTQGPVASIGGNPFA 695
>gb|ADN33979.1| GTPase activating protein [Cucumis melo subsp. melo]
Length = 674
Score = 307 bits (787), Expect = 7e-81
Identities = 185/387 (47%), Positives = 220/387 (56%), Gaps = 15/387 (3%)
Frame = -2
Query: 2296 RKAEERNEKIIRGLMKLPPNRRCINCNALGPQYVCTDFWTFVCMICSGIHREFTHRVKSV 2117
RK EERNE+IIRGLMKLPPNRRCINCN LGPQYVCT+FWTFVCM CSGIHREFTHRVKSV
Sbjct: 4 RKEEERNERIIRGLMKLPPNRRCINCNGLGPQYVCTNFWTFVCMTCSGIHREFTHRVKSV 63
Query: 2116 SMSKFTAQEVEALERGGNQRARETFLHDWDPQRHRFPVSSNADKVREFIKNVYVDRKYAS 1937
SM+KFT QEVEAL+ GGNQRARE +L DWD QR R PV+SN +K+REFIKNVYVDRKYA
Sbjct: 64 SMAKFTFQEVEALQNGGNQRAREIYLKDWDFQRQRLPVNSNVEKIREFIKNVYVDRKYAG 123
Query: 1936 KKTCEKPPRNLQDHKTHEDLTRRAXXXXXXXXXXXXXXXYEERRYGKQAFALTRKAGSDR 1757
+T EKPPR++Q + HED TRRA YE+R+YGKQA +LTRK GSDR
Sbjct: 124 GRTSEKPPRDMQSLRIHEDETRRASSYHSYSQSPPYDYQYEDRKYGKQAASLTRKPGSDR 183
Query: 1756 GL---KTSSS-FSPGRLGDYDIEEKFKKGHXXXXXXXXXXXSAGDPCRSYNQSPTTERGI 1589
G K S S FSPGRL D +++F S GDP RS SP ++
Sbjct: 184 GRYEGKVSGSVFSPGRLSDQTYDDRFANEGYASRVSDFSVSSGGDPFRSGAHSPNFQKDS 243
Query: 1588 KVSSPPSETCGDMLSLDEWNQKVNKFSDNSKVYEDR--SPHLKRTAS-------NSNSMP 1436
SSPP DML+ D+ +++ S + + D +RT S +SNS
Sbjct: 244 GFSSPPFPCARDMLN-DDTRHQISSMSAEANGHRDAYGISRPQRTMSSGSFGSIDSNSTS 302
Query: 1435 FSSVNSVKASGA--EQATGTHCKLTTXXXXXXXXXXSNYIGLDLFDTMFASQVXXXXXXX 1262
S NS + E H L I L F+ FA +
Sbjct: 303 LKSYNSAGVTDGVLEPEPIAHNNLDKMPASQQSSVPGVSISLSFFEEPFAPK-------- 354
Query: 1261 XXXXXXXXSGLFKSPEISSASPIDMFQ 1181
LF+S A P+D+FQ
Sbjct: 355 PVSAVTSSVDLFQSQASLPAPPVDLFQ 381
Score = 56.6 bits (135), Expect(2) = 1e-07
Identities = 33/98 (33%), Positives = 51/98 (52%), Gaps = 11/98 (11%)
Frame = -3
Query: 615 NTTQQWSAFHDS--------SAQVTYPSMPIQW---QNSPHNNVGIPSKSYQAEALSSTT 469
+T W+AF D+ Q T +P+Q + H G+ T+
Sbjct: 524 STDASWNAFEDAITDLSLQGGKQNTELQVPVQEFLPSSDGHLFFGVTEGE---RGTQMTS 580
Query: 468 NRSTNPFDIAYEYDVAPNNVFLDMSSMQGALPNSEMPT 355
+STNPFD+ Y+ D+ N+FLDMSS+Q ALPN+++P+
Sbjct: 581 GKSTNPFDLPYDPDMEQTNMFLDMSSLQSALPNAQLPS 618
Score = 27.7 bits (60), Expect(2) = 1e-07
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = -2
Query: 235 PSAQLASIQSEGPVAPVGGNPFA 167
P +Q+++I VA +GGNPFA
Sbjct: 652 PGSQISNITPPESVASIGGNPFA 674
>ref|XP_004136610.1| PREDICTED: probable ADP-ribosylation factor GTPase-activating protein
AGD14-like [Cucumis sativus]
gi|449522163|ref|XP_004168097.1| PREDICTED: probable
ADP-ribosylation factor GTPase-activating protein
AGD14-like [Cucumis sativus]
Length = 673
Score = 305 bits (780), Expect = 5e-80
Identities = 185/387 (47%), Positives = 220/387 (56%), Gaps = 15/387 (3%)
Frame = -2
Query: 2296 RKAEERNEKIIRGLMKLPPNRRCINCNALGPQYVCTDFWTFVCMICSGIHREFTHRVKSV 2117
RK EERNE+IIRGLMKLPPNRRCINCN LGPQYVCT+FWTFVCM CSGIHREFTHRVKSV
Sbjct: 4 RKEEERNERIIRGLMKLPPNRRCINCNGLGPQYVCTNFWTFVCMTCSGIHREFTHRVKSV 63
Query: 2116 SMSKFTAQEVEALERGGNQRARETFLHDWDPQRHRFPVSSNADKVREFIKNVYVDRKYAS 1937
SM+KF+ QEVEAL+ GGNQRARE +L DWD QR R PV+SN +K+REFIKNVYVDRKYA
Sbjct: 64 SMAKFSFQEVEALQNGGNQRAREIYLKDWDFQRQRLPVNSNVEKIREFIKNVYVDRKYAG 123
Query: 1936 KKTCEKPPRNLQDHKTHEDLTRRAXXXXXXXXXXXXXXXYEERRYGKQAFALTRKAGSDR 1757
+T EKPPR++Q + HED TRRA YE+R+YGKQA +LTRK GSDR
Sbjct: 124 GRTSEKPPRDMQSIRIHEDETRRASSYHSYSQSPPYDYQYEDRKYGKQAASLTRKPGSDR 183
Query: 1756 GL---KTSSS-FSPGRLGDYDIEEKFKKGHXXXXXXXXXXXSAGDPCRSYNQSPTTERGI 1589
G K S S FSPGRL D +++F S GDP RS SP ++
Sbjct: 184 GRYEGKVSGSVFSPGRLSDQTYDDRFVNEGYASRVSDFSVSSGGDPFRSGAHSPNFQKDS 243
Query: 1588 KVSSPPSETCGDMLSLDEWNQKVNKFSDNSKVYEDR--SPHLKRTAS-------NSNSMP 1436
SSPP DML+ D +Q ++ S + + D +RT S +SNS
Sbjct: 244 GFSSPPFHCARDMLNEDTRHQ-ISSMSAEANGHRDAYGISRPQRTMSSGSFGSIDSNSTS 302
Query: 1435 FSSVNSVKASGA--EQATGTHCKLTTXXXXXXXXXXSNYIGLDLFDTMFASQVXXXXXXX 1262
S NS + E H L I L F+ FA +
Sbjct: 303 LKSYNSAGLTDGVLEPEPIAHNNLDKMPSSQQSSVPGVSISLSFFEEPFAPK-------- 354
Query: 1261 XXXXXXXXSGLFKSPEISSASPIDMFQ 1181
LF+S A P+D+FQ
Sbjct: 355 PVSSATSSVDLFQSQASLPAPPVDLFQ 381
Score = 57.0 bits (136), Expect(2) = 9e-08
Identities = 33/98 (33%), Positives = 51/98 (52%), Gaps = 11/98 (11%)
Frame = -3
Query: 615 NTTQQWSAFHDS--------SAQVTYPSMPIQW---QNSPHNNVGIPSKSYQAEALSSTT 469
+T W+AF D+ Q T +P+Q + H G+ T+
Sbjct: 523 STDASWNAFEDAITDLSLQRGKQNTERQVPVQEFLPSSDGHLFFGVTEGE---RGTQMTS 579
Query: 468 NRSTNPFDIAYEYDVAPNNVFLDMSSMQGALPNSEMPT 355
+STNPFD+ Y+ D+ N+FLDMSS+Q ALPN+++P+
Sbjct: 580 GKSTNPFDLPYDPDMEQTNMFLDMSSLQSALPNAQLPS 617
Score = 27.7 bits (60), Expect(2) = 9e-08
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = -2
Query: 235 PSAQLASIQSEGPVAPVGGNPFA 167
P +Q+++I VA +GGNPFA
Sbjct: 651 PGSQISNITPPESVASIGGNPFA 673
>ref|XP_002530953.1| HIV-1 rev binding protein, hrbl, putative [Ricinus communis]
gi|223529468|gb|EEF31425.1| HIV-1 rev binding protein,
hrbl, putative [Ricinus communis]
Length = 692
Score = 303 bits (775), Expect = 2e-79
Identities = 174/322 (54%), Positives = 206/322 (63%), Gaps = 16/322 (4%)
Frame = -2
Query: 2296 RKAEERNEKIIRGLMKLPPNRRCINCNALGPQYVCTDFWTFVCMICSGIHREFTHRVKSV 2117
RK EERNEKIIRGLMKLPPNRRCINCN+LGPQ+VCT+FWTFVCM CSGIHREFTHRVKSV
Sbjct: 4 RKEEERNEKIIRGLMKLPPNRRCINCNSLGPQFVCTNFWTFVCMTCSGIHREFTHRVKSV 63
Query: 2116 SMSKFTAQEVEALERGGNQRARETFLHDWDPQRHRFPVSSNADKVREFIKNVYVDRKYAS 1937
SMSKFT+Q+VEAL+ GGNQRARE +L DWD QR R P +SN DK+REFIK++YVD+KYA
Sbjct: 64 SMSKFTSQDVEALQNGGNQRAREIYLKDWDQQRQRLPDNSNVDKIREFIKSIYVDKKYAG 123
Query: 1936 KKTCEKPPRNLQDHKTHEDLTRRAXXXXXXXXXXXXXXXYEERRYGKQAFALTRKAGSDR 1757
KT +KPPR+LQ ++HED TRRA YE+RRYGKQA LTRK GSDR
Sbjct: 124 GKTHDKPPRDLQRMRSHEDETRRASSYHSYSQSPPYDYQYEDRRYGKQAGILTRKPGSDR 183
Query: 1756 GLKTS--SSF--SPGRLGDYDIEEKFKKGHXXXXXXXXXXXSAGDPCRSYNQSPTTERGI 1589
GL SSF SP RL D E++F D S T
Sbjct: 184 GLYVGKMSSFICSPTRLSDRMFEDRFAN--------EGSVSGVSDYSVSSGGELVTTESP 235
Query: 1588 KVSSPPSETCGDMLSLDEWNQKVNKFSD-NSKVYEDRSPHLKRTAS-------NSNSMPF 1433
SSPP +T ++L D ++++ FS+ N K + PH +R+AS +SNS+
Sbjct: 236 NYSSPPIQTPKNILGEDVQQRRIDLFSEANFKRGAEGIPHSQRSASLGSIGSFDSNSVSV 295
Query: 1432 SSVNSVK----ASGAEQATGTH 1379
S NS S EQATGTH
Sbjct: 296 KSFNSSSLADIVSEPEQATGTH 317