BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005263
         (1638 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAC09580.1| protein kinase (PK) [Fagus sylvatica]                 632   e-178
emb|CAN83589.1| hypothetical protein VITISV_022074 [Vitis vinifera]   623   e-176
ref|XP_002310003.1| predicted protein [Populus trichocarpa] gi|2...   610   e-172
ref|XP_002509466.1| protein-tyrosine kinase, putative [Ricinus c...   610   e-172
ref|XP_003529330.1| PREDICTED: serine/threonine-protein kinase H...   603   e-170

>emb|CAC09580.1| protein kinase (PK) [Fagus sylvatica]
          Length = 480

 Score =  632 bits (1629), Expect = e-178
 Identities = 325/488 (66%), Positives = 382/488 (78%), Gaps = 12/488 (2%)
 Frame = +3

Query: 39   MGEE-SSWVRRTNYSHTIYHRLDSGRLTLESSRLASFNS--HPNRAPGVKERPAKLLSVT 209
            MGEE +SW+RRT +SHT+ HRLD+       SRLASF     P R  G+K RP    S  
Sbjct: 1    MGEEGNSWIRRTKFSHTVCHRLDA-------SRLASFPIIIRPERNSGLKSRPGATSSSE 53

Query: 210  PIQLNPKTNKLRAVSPHPETKLSDTFKEARSEKKRFSTPHPIRRGS-----GKVLEKDTL 374
              +     NK R++SP PE++LSD FKEARSE KRF TP P RR       GK + KD  
Sbjct: 54   --KFGTIYNKQRSLSPLPESRLSDVFKEARSETKRFMTPLPRRREQAKGVMGKFVHKDFH 111

Query: 375  AEKTADSKQLSKS--LKHFS-MKFHDKSKSRKESAWKKFFDHGVGKVTFVESAEEWMVDL 545
              K +DSK L+ S  L+H S MK +DKSK RKES+W K+FDHG G+V  VE+++EW VDL
Sbjct: 112  EVKASDSKSLANSSALRHLSSMKVNDKSKYRKESSWAKYFDHGGGRVNAVETSDEWTVDL 171

Query: 546  SKLYLGLRFAHGAHSQLYHGIYKEEPVAVKMIRVPDDIYDGSLGARLEKQFTREVTLLSC 725
            SKL+LGL+FAHGAHS+LYHGIY +EPVAVK+IRVP+D  +G+LGARLEKQF REVTLLS 
Sbjct: 172  SKLFLGLKFAHGAHSRLYHGIYNDEPVAVKIIRVPEDDENGALGARLEKQFNREVTLLSR 231

Query: 726  LHHQNVIKFIGACRKPPVFCIMTEYLSEGSLRAYLHKLE-ETLPLQKIISMALDIVRGMT 902
            LH  N+IKF+ ACRKPPV+C++TEYLSEGSLRAYLHKLE ++LPLQK+I+ ALDI RGM 
Sbjct: 232  LHFHNIIKFVAACRKPPVYCVVTEYLSEGSLRAYLHKLERKSLPLQKLIAFALDIARGME 291

Query: 903  FVHSQGVIHRDLKPENILINQDFQLKIADFGIACRDTSCDSLADDTGTYRWMAPEMIKRK 1082
            ++HSQGVIHRDLKPEN+LI+Q+F LKIADFGIAC +  CDSLADD GTYRWMAPEMIK K
Sbjct: 292  YIHSQGVIHRDLKPENVLIDQEFHLKIADFGIACEEAYCDSLADDPGTYRWMAPEMIKHK 351

Query: 1083 SYSRKVDVYGFGLILWEMVAGSIPYEDMTPIQAAFAVVNKNLRPAIPDSCPAVMKTLIEQ 1262
            SY RKVDVY FGLILWEMVAG+IPYEDM P+QAAFAVVNKNLRP IP  CP  M+ LIEQ
Sbjct: 352  SYGRKVDVYSFGLILWEMVAGTIPYEDMNPVQAAFAVVNKNLRPVIPRYCPPAMRALIEQ 411

Query: 1263 CWSAYPDKRPDFCQVLKVLEQLEASLASNGSLDLVQNLTCHDHKKGIFHLIHKLGPVHHT 1442
            CWS   +KRP+F QV+KVLEQ E+SLA +G+L+LVQ+LTC DHKKG+ H IHKLGPVH  
Sbjct: 412  CWSLQSEKRPEFWQVVKVLEQFESSLARDGTLNLVQSLTCQDHKKGLLHWIHKLGPVHPN 471

Query: 1443 PSSMPKPK 1466
              SMPKPK
Sbjct: 472  -GSMPKPK 478


>emb|CAN83589.1| hypothetical protein VITISV_022074 [Vitis vinifera]
          Length = 489

 Score =  623 bits (1606), Expect = e-176
 Identities = 314/495 (63%), Positives = 387/495 (78%), Gaps = 18/495 (3%)
 Frame = +3

Query: 39   MGEESSWVRRTNYSHTIYHRLDSGRLTLESSRLASF--NSHPNRAPGVKERPAK-LLSVT 209
            M + +SW+RRT +SHTI+HRLDS       +RLASF     P R  G+K RP +  L++ 
Sbjct: 1    MEDANSWIRRTKFSHTIFHRLDS-------TRLASFPLTISPERNSGLKSRPQRGSLNLK 53

Query: 210  PIQLNPK------TNKLRAVSPHPETKLSDTFKEARSEKKRFSTPHPIRRGS-----GKV 356
                +P+      TNK R++SP PE+KLSD FKEARS++KRFSTP P R+       GK+
Sbjct: 54   SEPSDPQGRRNAFTNKQRSLSPLPESKLSDEFKEARSDRKRFSTPVPRRKEHEKGIMGKL 113

Query: 357  LEKDTLAEKTADSKQLSKS--LKHF-SMKFHDKSKSRKESAWKKFFDHGVGKVTFVESAE 527
              KD+   K ++S + S +  L+H  S K HDK+K RKE +W K+FDHG G+VT VE+A+
Sbjct: 114  FSKDSQDPKASNSNKQSNTSPLRHLTSTKNHDKAKFRKEPSWIKYFDHGGGRVTAVEAAD 173

Query: 528  EWMVDLSKLYLGLRFAHGAHSQLYHGIYKEEPVAVKMIRVPDDIYDGSLGARLEKQFTRE 707
            EWM DLSKLYLGLRF+HGAHS+LYHG+Y+E+PVAVK+I VPD+  +G+L ++LEKQF  E
Sbjct: 174  EWMADLSKLYLGLRFSHGAHSRLYHGVYEEQPVAVKVIMVPDEEENGALASKLEKQFNGE 233

Query: 708  VTLLSCLHHQNVIKFIGACRKPPVFCIMTEYLSEGSLRAYLHKLE-ETLPLQKIISMALD 884
            V+ LS LHHQNVIKF+ A R+PPVF ++TEYLSEGSLRAYLHKLE ++LPL+K+I++ALD
Sbjct: 234  VSCLSRLHHQNVIKFVAAWRRPPVFVVITEYLSEGSLRAYLHKLEHKSLPLEKLITIALD 293

Query: 885  IVRGMTFVHSQGVIHRDLKPENILINQDFQLKIADFGIACRDTSCDSLADDTGTYRWMAP 1064
            I RGM ++HSQG+IHRDLKPEN+L+ +DF +KIADFGIAC +  CDSLADD GTYRWMAP
Sbjct: 294  IARGMEYIHSQGIIHRDLKPENVLVTKDFHMKIADFGIACEEAYCDSLADDPGTYRWMAP 353

Query: 1065 EMIKRKSYSRKVDVYGFGLILWEMVAGSIPYEDMTPIQAAFAVVNKNLRPAIPDSCPAVM 1244
            EMIK KSY RKVDVY FGLILWEMVAG+IPYEDM PIQAAFAVVNKNLRP IP  CP  M
Sbjct: 354  EMIKHKSYGRKVDVYSFGLILWEMVAGTIPYEDMAPIQAAFAVVNKNLRPVIPVDCPPAM 413

Query: 1245 KTLIEQCWSAYPDKRPDFCQVLKVLEQLEASLASNGSLDLVQNLTCHDHKKGIFHLIHKL 1424
            + LIEQCWS +PDKRP+F QV+KVLEQ ++SLA +G+L+LV+  TC DHKKG+ H I KL
Sbjct: 414  RALIEQCWSLHPDKRPEFWQVVKVLEQFKSSLALDGTLNLVRYATCQDHKKGLLHWIQKL 473

Query: 1425 GPVHHTPSSMPKPKF 1469
            GPVHH  SSMPKPKF
Sbjct: 474  GPVHHNTSSMPKPKF 488


>ref|XP_002310003.1| predicted protein [Populus trichocarpa] gi|222852906|gb|EEE90453.1|
            predicted protein [Populus trichocarpa]
          Length = 464

 Score =  610 bits (1574), Expect = e-172
 Identities = 312/483 (64%), Positives = 368/483 (76%), Gaps = 8/483 (1%)
 Frame = +3

Query: 45   EESSWVRRTNYSHTIYHRLDSGRLTLESSRLASFNSHPNRAPGVKERPAKLLSVTPIQLN 224
            E +SW+RRTN+SHT+YHRLDS       SR+ASF             P  + +   IQ N
Sbjct: 4    EANSWIRRTNFSHTVYHRLDS-------SRMASF-------------PINIQTERIIQRN 43

Query: 225  PKTNKLRAVSPHPETKLSDTFKEARSEKKRFSTPHPIRRGS-----GKVLEKDTLAEKTA 389
            P +NK R++SP P+T L DTFKEARS  KRFSTP P R+       GK L K+    K  
Sbjct: 44   PLSNKQRSLSPLPKTILPDTFKEARSVNKRFSTPLPRRKDQDMGIIGKFLHKEL---KET 100

Query: 390  DSKQLSKSLKHFSMKF--HDKSKSRKESAWKKFFDHGVGKVTFVESAEEWMVDLSKLYLG 563
              K  +  L+H ++    H+KS+S+KESAW K+FDHG GKV  VE+A+E  VDLSKL+LG
Sbjct: 101  KEKLNTSPLRHLALMKGGHEKSRSKKESAWTKYFDHGGGKVNAVEAADECSVDLSKLFLG 160

Query: 564  LRFAHGAHSQLYHGIYKEEPVAVKMIRVPDDIYDGSLGARLEKQFTREVTLLSCLHHQNV 743
            LRFAHGAHS+LYHG+YK+EPVAVK+IRVPDD  +G+L  RLE QF REV LLS LHH NV
Sbjct: 161  LRFAHGAHSRLYHGLYKDEPVAVKLIRVPDDDENGNLAIRLENQFNREVMLLSHLHHPNV 220

Query: 744  IKFIGACRKPPVFCIMTEYLSEGSLRAYLHKLE-ETLPLQKIISMALDIVRGMTFVHSQG 920
            IKF+ ACRKPPV+C++TEYLSEGSLRAYLHKLE +TL L K++++ALDI RGM ++HSQG
Sbjct: 221  IKFVAACRKPPVYCVITEYLSEGSLRAYLHKLEHKTLSLGKLMTIALDIARGMEYIHSQG 280

Query: 921  VIHRDLKPENILINQDFQLKIADFGIACRDTSCDSLADDTGTYRWMAPEMIKRKSYSRKV 1100
            VIHRDLKPEN+LI+Q+F LKIADFGIAC D  CDSLADD GTYRWMAPEMIK+KSY RKV
Sbjct: 281  VIHRDLKPENVLIDQEFHLKIADFGIACGDAYCDSLADDPGTYRWMAPEMIKKKSYGRKV 340

Query: 1101 DVYGFGLILWEMVAGSIPYEDMTPIQAAFAVVNKNLRPAIPDSCPAVMKTLIEQCWSAYP 1280
            DVY FGLILWEMVAG+IPYEDMTPIQAAFAVVNKN RP IP  CPA M  LIEQCWS  P
Sbjct: 341  DVYSFGLILWEMVAGTIPYEDMTPIQAAFAVVNKNSRPVIPRDCPAAMGALIEQCWSLQP 400

Query: 1281 DKRPDFCQVLKVLEQLEASLASNGSLDLVQNLTCHDHKKGIFHLIHKLGPVHHTPSSMPK 1460
            DKRP+F Q++KVLEQ E+SLA +G+L+LV N  C DHKKG+ H I KLG V+   + MPK
Sbjct: 401  DKRPEFWQIVKVLEQFESSLAHDGTLNLVPNPACQDHKKGMLHWIQKLGTVNPNSTPMPK 460

Query: 1461 PKF 1469
            PKF
Sbjct: 461  PKF 463


>ref|XP_002509466.1| protein-tyrosine kinase, putative [Ricinus communis]
            gi|223549365|gb|EEF50853.1| protein-tyrosine kinase,
            putative [Ricinus communis]
          Length = 496

 Score =  610 bits (1573), Expect = e-172
 Identities = 302/498 (60%), Positives = 378/498 (75%), Gaps = 23/498 (4%)
 Frame = +3

Query: 45   EESSWVRRTNYSHTIYHRLDSGRLT-----LESSRLASFNSHPNRAPGV----------- 176
            E SSW+RRT +SHT+ HRLDS RL      ++  R +   S P  +              
Sbjct: 4    ENSSWIRRTKFSHTVCHRLDSSRLASLALAVQEERNSGLKSRPGTSSPNRKSLYFSNQRS 63

Query: 177  -----KERPAKLLSVTPIQLNPKTNKLRAVSPHPETKLSDTFKEARSEKKRFSTPHPIRR 341
                 K+  + + S + IQ NP TNKLR++SP P+T LSDTFKEARS++KRFSTPHP  +
Sbjct: 64   ASFPNKKSSSSVTSNSQIQRNPVTNKLRSLSPLPQTALSDTFKEARSDRKRFSTPHPRSK 123

Query: 342  GSGKVLEKDTL-AEKTADSKQLSKSLKHFSMKFHDKSKSRKESAWKKFFDHGVGKVTFVE 518
               K   K +  A  T+  +QL+      S+K H+K KS+K+SAW K+FDH  G+V  VE
Sbjct: 124  DQDKGFAKPSKHASNTSPLRQLA------SLKGHEKLKSKKDSAWTKYFDHAGGRVNAVE 177

Query: 519  SAEEWMVDLSKLYLGLRFAHGAHSQLYHGIYKEEPVAVKMIRVPDDIYDGSLGARLEKQF 698
            +A+E+ VD+SKL+LGLRFAHGAHS+LYHG+YK+EPVAVK+IR PDD  +G+L  RL+ Q+
Sbjct: 178  AADEFSVDMSKLFLGLRFAHGAHSRLYHGVYKDEPVAVKIIRAPDDDDNGTLSIRLKNQY 237

Query: 699  TREVTLLSCLHHQNVIKFIGACRKPPVFCIMTEYLSEGSLRAYLHKLE-ETLPLQKIISM 875
             REVTLLS LHH NVIKF+ AC+ PPV+C++TEYLSEGSLRAYLHKLE ++LPL+K+I++
Sbjct: 238  DREVTLLSRLHHPNVIKFVAACKMPPVYCVITEYLSEGSLRAYLHKLEHKSLPLEKLIAI 297

Query: 876  ALDIVRGMTFVHSQGVIHRDLKPENILINQDFQLKIADFGIACRDTSCDSLADDTGTYRW 1055
            ALDI RGM ++HSQ +IHRDLKPEN+LI+Q+F++KIADFGIAC +  CDSLADD GTYRW
Sbjct: 298  ALDIARGMEYIHSQSIIHRDLKPENVLIDQEFRMKIADFGIACEEAYCDSLADDPGTYRW 357

Query: 1056 MAPEMIKRKSYSRKVDVYGFGLILWEMVAGSIPYEDMTPIQAAFAVVNKNLRPAIPDSCP 1235
            MAPEMIK+KSY ++VDVY FGLILWE+VAG+IPYEDM PIQAAFAVVNKNLRP IP  C 
Sbjct: 358  MAPEMIKKKSYGKRVDVYSFGLILWELVAGTIPYEDMNPIQAAFAVVNKNLRPVIPRDCH 417

Query: 1236 AVMKTLIEQCWSAYPDKRPDFCQVLKVLEQLEASLASNGSLDLVQNLTCHDHKKGIFHLI 1415
              M+ LIEQCWS  PDKRP+F Q++KVLEQ  +SLA +G+L+LVQN TC DHKKG+ H I
Sbjct: 418  PAMRALIEQCWSLQPDKRPEFWQIVKVLEQFGSSLARDGTLNLVQNPTCQDHKKGLLHWI 477

Query: 1416 HKLGPVHHTPSSMPKPKF 1469
             KLGP+H + S MPKPKF
Sbjct: 478  QKLGPMHPSSSPMPKPKF 495


>ref|XP_003529330.1| PREDICTED: serine/threonine-protein kinase HT1-like [Glycine max]
          Length = 498

 Score =  603 bits (1555), Expect = e-170
 Identities = 311/502 (61%), Positives = 373/502 (74%), Gaps = 25/502 (4%)
 Frame = +3

Query: 39   MGEESS--WVRRTNYSHTIYHRLDSGRLTLESSRLASFNSHPN--RAPGVKERPAKLLSV 206
            M E+SS  W+RR  +SHT+ HRL+       SS   SF+  P    +  +K RP    + 
Sbjct: 1    MDEDSSNSWIRRAKFSHTVCHRLN-----YSSSGFGSFSIQPRAQHSSSLKSRPEVAFAS 55

Query: 207  ---TPIQLNPKTNKLRAVSPHPETKLSDTFKEARSEKKRFSTPHPIRRGS-------GKV 356
               +  Q  P  NK R++SP P+T LS+TFKEAR E+KRFSTP P R+         GK+
Sbjct: 56   KASSQAQKKPIMNKQRSLSPLPQTSLSETFKEARHEQKRFSTPGPRRKEQDKDKRIMGKL 115

Query: 357  LEKDT--LAEKTADSKQL------SKSLKHF-SMKFHDKSKS-RKESAWKKFFDHGVGKV 506
            L KD+     K+  S  +      S  ++H  SMK  DKSK  RK+  W K+FDHG GKV
Sbjct: 116  LNKDSHVFNSKSNHSSPITTKSPYSSPIRHLASMKLSDKSKQHRKDLGWTKYFDHGGGKV 175

Query: 507  TFVESAEEWMVDLSKLYLGLRFAHGAHSQLYHGIYKEEPVAVKMIRVPDDIYDGSLGARL 686
            T VE+AEEW VDLSKL++G+RFAHGAHS+LYHG+YK+E VAVK+I VPDD  +G L  RL
Sbjct: 176  TAVETAEEWNVDLSKLFVGVRFAHGAHSRLYHGMYKDEAVAVKIITVPDDDENGMLADRL 235

Query: 687  EKQFTREVTLLSCLHHQNVIKFIGACRKPPVFCIMTEYLSEGSLRAYLHKLE-ETLPLQK 863
            EKQF REV+LLS LHHQNVIKF+ ACRKPPV+C++TEYLSEGSLR+YLHKLE +T+PL+K
Sbjct: 236  EKQFIREVSLLSRLHHQNVIKFVAACRKPPVYCVITEYLSEGSLRSYLHKLERKTIPLEK 295

Query: 864  IISMALDIVRGMTFVHSQGVIHRDLKPENILINQDFQLKIADFGIACRDTSCDSLADDTG 1043
            +I+ ALDI RGM ++HSQGVIHRDLKPEN+LI +DF LKIADFGIAC +  CD  ADD G
Sbjct: 296  LIAFALDIARGMEYIHSQGVIHRDLKPENVLIKEDFHLKIADFGIACEEAYCDLFADDPG 355

Query: 1044 TYRWMAPEMIKRKSYSRKVDVYGFGLILWEMVAGSIPYEDMTPIQAAFAVVNKNLRPAIP 1223
            TYRWMAPEMIKRKSY RKVDVY FGLILWEMV G+IPYEDMTPIQAAFAVVNKN+RP IP
Sbjct: 356  TYRWMAPEMIKRKSYGRKVDVYSFGLILWEMVTGTIPYEDMTPIQAAFAVVNKNVRPVIP 415

Query: 1224 DSCPAVMKTLIEQCWSAYPDKRPDFCQVLKVLEQLEASLASNGSLDLVQNLTCHDHKKGI 1403
             +CP  M+ LIEQCWS +PDKRP+F QV+KVLEQ E+SLA +G+L LV+N  C DHKKG+
Sbjct: 416  SNCPPAMRALIEQCWSLHPDKRPEFWQVVKVLEQFESSLAHDGTLTLVENPCCPDHKKGL 475

Query: 1404 FHLIHKLGPVHHTPSSMPKPKF 1469
             H I KLGPVHH    +PKPKF
Sbjct: 476  LHWIQKLGPVHHNNGPVPKPKF 497