BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005248
         (284 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002874883.1| protein binding protein [Arabidopsis lyrata ...    67   1e-09
ref|NP_001184899.1| zinc ion binding protein [Arabidopsis thalia...    64   1e-08
ref|NP_192209.2| putative E3 ubiquitin-protein ligase RF298 [Ara...    64   1e-08
ref|XP_002277269.1| PREDICTED: putative E3 ubiquitin-protein lig...    64   1e-08
ref|XP_002312723.1| predicted protein [Populus trichocarpa] gi|2...    64   1e-08

>ref|XP_002874883.1| protein binding protein [Arabidopsis lyrata subsp. lyrata]
           gi|297320720|gb|EFH51142.1| protein binding protein
           [Arabidopsis lyrata subsp. lyrata]
          Length = 802

 Score = 67.4 bits (163), Expect = 1e-09
 Identities = 35/90 (38%), Positives = 49/90 (54%)
 Frame = +2

Query: 14  SLTEFPRYELLTEKLANEIGNIGPSVEDDVKEYILADWDDPFECQFEELLSDTLYTTFRE 193
           SLTEFPRYEL + K  + +     + +   +E     WDDPF CQ E+LLS  L T FR 
Sbjct: 39  SLTEFPRYELHSLKSQSPLSENDSNGQLKAEESDSVGWDDPFACQLEQLLSSNLLTLFRS 98

Query: 194 TIKEVAEHGYSEEAAEQAILKRGMCYGDTD 283
            + ++ + GYSE+   +AI    +  G  D
Sbjct: 99  AMNQIMDCGYSEDVVLKAISSSRLYCGGND 128


>ref|NP_001184899.1| zinc ion binding protein [Arabidopsis thaliana]
           gi|75217041|sp|Q9ZVT8.1|RF4_ARATH RecName: Full=Putative
           E3 ubiquitin-protein ligase RF4; AltName: Full=RING
           finger protein 4 gi|3850566|gb|AAC72106.1| F15K9.3
           [Arabidopsis thaliana] gi|332189443|gb|AEE27564.1| zinc
           ion binding protein [Arabidopsis thaliana]
          Length = 823

 Score = 64.3 bits (155), Expect = 1e-08
 Identities = 36/95 (37%), Positives = 56/95 (58%), Gaps = 7/95 (7%)
 Frame = +2

Query: 14  SLTEFPRYELLTEKLANE------IGNIGPSVEDDVKEYILADWDDPFECQFEELLSDTL 175
           SLTEFP YEL + K  N       +G +   ++ +V E +  +WDDPF C  EELLS  L
Sbjct: 48  SLTEFPPYELPSLKPQNHLSGNGSVGEVSNQLQVEVSESV--EWDDPFACHLEELLSSNL 105

Query: 176 YTTFRETIKEVAEHGYSEEAAEQAILK-RGMCYGD 277
            T F +T+K++ + GY+++   +A+ + R  C G+
Sbjct: 106 LTLFLDTMKQLIDLGYTDDEVLKAVSRCRLYCGGN 140


>ref|NP_192209.2| putative E3 ubiquitin-protein ligase RF298 [Arabidopsis thaliana]
           gi|42572815|ref|NP_974504.1| putative E3
           ubiquitin-protein ligase RF298 [Arabidopsis thaliana]
           gi|122229999|sp|Q0WPJ7.1|RF298_ARATH RecName:
           Full=Putative E3 ubiquitin-protein ligase RF298;
           AltName: Full=RING finger protein 298
           gi|110738035|dbj|BAF00952.1| hypothetical protein
           [Arabidopsis thaliana] gi|332656858|gb|AEE82258.1|
           putative E3 ubiquitin-protein ligase RF298 [Arabidopsis
           thaliana] gi|332656859|gb|AEE82259.1| putative E3
           ubiquitin-protein ligase RF298 [Arabidopsis thaliana]
          Length = 814

 Score = 63.9 bits (154), Expect = 1e-08
 Identities = 35/90 (38%), Positives = 47/90 (52%)
 Frame = +2

Query: 14  SLTEFPRYELLTEKLANEIGNIGPSVEDDVKEYILADWDDPFECQFEELLSDTLYTTFRE 193
           SLTEFPRYEL + K  + +     + +   +E     WDDPF C  E LLS  L T FR 
Sbjct: 49  SLTEFPRYELHSFKSQSPLCENDSNGQLKAEESDSVGWDDPFACHLEGLLSSNLLTLFRS 108

Query: 194 TIKEVAEHGYSEEAAEQAILKRGMCYGDTD 283
            + ++ + GYSE+   +AI       G TD
Sbjct: 109 AMNQIMDCGYSEDVVLKAISSSRFYCGGTD 138


>ref|XP_002277269.1| PREDICTED: putative E3 ubiquitin-protein ligase RF298-like [Vitis
           vinifera]
          Length = 893

 Score = 63.9 bits (154), Expect = 1e-08
 Identities = 31/75 (41%), Positives = 45/75 (60%)
 Frame = +2

Query: 59  ANEIGNIGPSVEDDVKEYILADWDDPFECQFEELLSDTLYTTFRETIKEVAEHGYSEEAA 238
           ++E+G   P  E +  ++  ADW D  E Q EEL+   L T F+  IK++   GYSEE A
Sbjct: 99  SSEVGPSQPRDELEADDFQDADWSDLTESQLEELVLSNLDTIFKSAIKKIVACGYSEEVA 158

Query: 239 EQAILKRGMCYGDTD 283
            +A+L+ G+CYG  D
Sbjct: 159 TKAVLRSGLCYGCKD 173


>ref|XP_002312723.1| predicted protein [Populus trichocarpa] gi|222852543|gb|EEE90090.1|
           predicted protein [Populus trichocarpa]
          Length = 762

 Score = 63.9 bits (154), Expect = 1e-08
 Identities = 37/95 (38%), Positives = 51/95 (53%), Gaps = 5/95 (5%)
 Frame = +2

Query: 5   MLPSLTEFPRYELLTEKL-----ANEIGNIGPSVEDDVKEYILADWDDPFECQFEELLSD 169
           M  +  E P YE   EK      ++E+G   P  E + +E   ADW D  E Q EEL+  
Sbjct: 28  MSSAQNECPGYEFSAEKFEAAPGSSEVGPSQPRGEVESEESHDADWSDLTESQLEELVLS 87

Query: 170 TLYTTFRETIKEVAEHGYSEEAAEQAILKRGMCYG 274
            L   F+  IK++   GY+EE A +AIL+ G+CYG
Sbjct: 88  NLDAIFKGAIKKIVACGYTEEEATKAILRSGLCYG 122