BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005232
         (1051 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAS90125.1| cytochrome P450 [Ammi majus]                           500   e-139
sp|D1MI46.1|C76BA_SWEMU RecName: Full=Geraniol 8-hydroxylase; Al...   462   e-128
sp|Q8VWZ7.1|C76B6_CATRO RecName: Full=Geraniol 8-hydroxylase; Al...   454   e-125
gb|AAQ05825.1|AF479661_1 cytochrome P450 [Pastinaca sativa]           449   e-124
gb|ADJ68324.1| geraniol 10-hydroxylase [Picrorhiza kurrooa]           446   e-123

>gb|AAS90125.1| cytochrome P450 [Ammi majus]
          Length = 497

 Score =  500 bits (1287), Expect = e-139
 Identities = 245/346 (70%), Positives = 289/346 (83%), Gaps = 6/346 (1%)
 Frame = +3

Query: 6    KVNELIGYVKMCSENGEAVDIGRAVFRTSLNLLSNTIFSKDIADPYQDSAKEMRGLIWDI 185
            KVNELI YV+ CS+ G+AVDIGRA FRTS NLLSNT+FSKD+ DPYQDSA+E + L W+I
Sbjct: 149  KVNELIAYVQKCSQTGDAVDIGRAAFRTSFNLLSNTVFSKDMVDPYQDSAQEFKDLAWNI 208

Query: 186  MVEAVKPNLVDFFPVLKKMDPQGVRQRMSRHFDSLMKILYGLIDER------GGHVENND 347
            MVEA  PNLVD+FPVLKKMDPQGV++RM+ +F  ++K+L GLI+ER      G  V+  D
Sbjct: 209  MVEAGSPNLVDYFPVLKKMDPQGVKRRMTGYFQKVIKMLDGLINERLALKGSGTTVDKTD 268

Query: 348  VLDELIKISQENPDEIDKIHIVHLIMDLFAAGTDTTSSTVEWGMAEVLKSSETTAKAKAE 527
            +LDELI ISQ NP+E+DKI + HL +D+F AGTDTTS+TVEWGMAE+L+S+ET  K KAE
Sbjct: 269  MLDELINISQVNPNEMDKILMEHLFVDIFVAGTDTTSNTVEWGMAEILRSTETMMKVKAE 328

Query: 528  LNQVLGKGKILEEADVSRLPYLRCIVTETLRLHPPVPFLLPRQTAEEAQLNGYKIPRNSQ 707
            L  ++GKG ILEE D+ RLP+L+CIV ETLRLHPP P LLPRQT EE +LNGY IP+NSQ
Sbjct: 329  LRHLVGKGVILEEGDIYRLPFLQCIVRETLRLHPPFPLLLPRQTEEETELNGYTIPKNSQ 388

Query: 708  VLVNAWAIGRDPDTWTNPLSFQPERFLDSEVDVRGKDFELIPFGAGRRICPGLSLAMRMV 887
            VLVNAWAIGRDP +W NP SF+PERFLDSEVDV+G+DFELIPFGAG RICPGL L MRMV
Sbjct: 389  VLVNAWAIGRDPVSWKNPSSFRPERFLDSEVDVKGQDFELIPFGAGIRICPGLPLVMRMV 448

Query: 888  PVMLGSLINCFDWELEGGTALSELDMDEKFGLTVQKLYPLCALATT 1025
            PVMLGSLINCFDWELEGG  L+ELDM+EK GL+V KL+PL  LAT+
Sbjct: 449  PVMLGSLINCFDWELEGGIPLNELDMEEKCGLSVAKLHPLRVLATS 494


>sp|D1MI46.1|C76BA_SWEMU RecName: Full=Geraniol 8-hydroxylase; AltName: Full=Cytochrome P450
            76B10; AltName: Full=Geraniol 10-hydroxylase;
            Short=SmG10H gi|269838629|gb|ACZ48680.1| geraniol
            10-hydroxylase [Swertia mussotii]
          Length = 495

 Score =  462 bits (1189), Expect = e-128
 Identities = 218/343 (63%), Positives = 281/343 (81%), Gaps = 4/343 (1%)
 Frame = +3

Query: 6    KVNELIGYVKMCSENGEAVDIGRAVFRTSLNLLSNTIFSKDIADPYQDSAKEMRGLIWDI 185
            KV ELI Y +  S+ G+A+D+GRA FRTSLNLLSNT+FSKD+ DPY DSAKE + L+W++
Sbjct: 151  KVQELIAYCRKSSQTGDAIDVGRAAFRTSLNLLSNTMFSKDLTDPYSDSAKEFKDLVWNV 210

Query: 186  MVEAVKPNLVDFFPVLKKMDPQGVRQRMSRHFDSLMKILYGLIDERGGHVE----NNDVL 353
            MVEA KPNLVD+FP+L K+DPQG+R+RM+ HF  ++++  GLIDER    +    N+DVL
Sbjct: 211  MVEAGKPNLVDYFPLLDKVDPQGIRKRMTIHFGKILELFGGLIDERLQQKKAKGVNDDVL 270

Query: 354  DELIKISQENPDEIDKIHIVHLIMDLFAAGTDTTSSTVEWGMAEVLKSSETTAKAKAELN 533
            D L+  S+E+P+EID+ HI  + +DLF AGTDTTSST+EW M+E+LK+ E    A+AEL 
Sbjct: 271  DVLLTTSEESPEEIDRTHIQRMCLDLFVAGTDTTSSTLEWAMSEMLKNPEKMKAAQAELA 330

Query: 534  QVLGKGKILEEADVSRLPYLRCIVTETLRLHPPVPFLLPRQTAEEAQLNGYKIPRNSQVL 713
            QV+GKGK +EEAD++RLPYLRC + ETLR+HPPVP L+PR+T +E ++ GY +P+NSQVL
Sbjct: 331  QVIGKGKAVEEADLARLPYLRCAIKETLRIHPPVPLLIPRRTEQEVEVCGYTVPKNSQVL 390

Query: 714  VNAWAIGRDPDTWTNPLSFQPERFLDSEVDVRGKDFELIPFGAGRRICPGLSLAMRMVPV 893
            VN WAI RD   W +PLSF+PERFL+SE+++RGKDFELIPFGAGRRICPGL LA+RMVPV
Sbjct: 391  VNVWAISRDDAIWKDPLSFKPERFLESELEMRGKDFELIPFGAGRRICPGLPLAVRMVPV 450

Query: 894  MLGSLINCFDWELEGGTALSELDMDEKFGLTVQKLYPLCALAT 1022
            MLGSL+N FDW+LEGG A  +LDM+EKFG+T+QK +PL A+AT
Sbjct: 451  MLGSLLNSFDWKLEGGIAPKDLDMEEKFGITLQKAHPLRAVAT 493


>sp|Q8VWZ7.1|C76B6_CATRO RecName: Full=Geraniol 8-hydroxylase; AltName: Full=Cytochrome P450
            76B6; AltName: Full=Geraniol 10-hydroxylase; Short=CrG10H
            gi|17065916|emb|CAC80883.1| geraniol 10-hydroxylase
            [Catharanthus roseus]
          Length = 493

 Score =  454 bits (1168), Expect = e-125
 Identities = 212/345 (61%), Positives = 279/345 (80%), Gaps = 4/345 (1%)
 Frame = +3

Query: 6    KVNELIGYVKMCSENGEAVDIGRAVFRTSLNLLSNTIFSKDIADPYQDSAKEMRGLIWDI 185
            KV ELI Y +  S++GEAVD+GRA FRTSLNLLSN IFSKD+ DPY DSAKE + L+W+I
Sbjct: 149  KVQELIAYCRKNSQSGEAVDVGRAAFRTSLNLLSNLIFSKDLTDPYSDSAKEFKDLVWNI 208

Query: 186  MVEAVKPNLVDFFPVLKKMDPQGVRQRMSRHFDSLMKILYGLIDER----GGHVENNDVL 353
            MVEA KPNLVDFFP+L+K+DPQG+R RM+ HF  ++K+  GL++ER        E NDVL
Sbjct: 209  MVEAGKPNLVDFFPLLEKVDPQGIRHRMTIHFGEVLKLFGGLVNERLEQRRSKGEKNDVL 268

Query: 354  DELIKISQENPDEIDKIHIVHLIMDLFAAGTDTTSSTVEWGMAEVLKSSETTAKAKAELN 533
            D L+  SQE+P+EID+ HI  + +DLF AGTDTTSST+EW M+E+LK+ +   K + EL 
Sbjct: 269  DVLLTTSQESPEEIDRTHIERMCLDLFVAGTDTTSSTLEWAMSEMLKNPDKMKKTQDELA 328

Query: 534  QVLGKGKILEEADVSRLPYLRCIVTETLRLHPPVPFLLPRQTAEEAQLNGYKIPRNSQVL 713
            QV+G+GK +EE+D++RLPYLRC++ ETLR+HPPVPFL+PR+  +  ++ GY +P+ SQVL
Sbjct: 329  QVIGRGKTIEESDINRLPYLRCVMKETLRIHPPVPFLIPRKVEQSVEVCGYNVPKGSQVL 388

Query: 714  VNAWAIGRDPDTWTNPLSFQPERFLDSEVDVRGKDFELIPFGAGRRICPGLSLAMRMVPV 893
            VNAWAIGRD   W + L+F+PERF++SE+D+RG+DFELIPFGAGRRICPGL LA+R VP+
Sbjct: 389  VNAWAIGRDETVWDDALAFKPERFMESELDIRGRDFELIPFGAGRRICPGLPLALRTVPL 448

Query: 894  MLGSLINCFDWELEGGTALSELDMDEKFGLTVQKLYPLCALATTV 1028
            MLGSL+N F+W+LEGG A  +LDM+EKFG+T+QK +PL A+ +T+
Sbjct: 449  MLGSLLNSFNWKLEGGMAPKDLDMEEKFGITLQKAHPLRAVPSTL 493


>gb|AAQ05825.1|AF479661_1 cytochrome P450 [Pastinaca sativa]
          Length = 496

 Score =  449 bits (1156), Expect = e-124
 Identities = 219/345 (63%), Positives = 279/345 (80%), Gaps = 5/345 (1%)
 Frame = +3

Query: 6    KVNELIGYVKMCSENGEAVDIGRAVFRTSLNLLSNTIFSKDIADPYQDS-AKEMRGLIWD 182
            KV++LI Y + CS++GEAVDIG A F TSLNL+SNTIFSKD+ D Y++S AK  R L+W+
Sbjct: 146  KVHDLIRYCEKCSQSGEAVDIGGAAFLTSLNLMSNTIFSKDMIDSYENSEAKVFRDLVWN 205

Query: 183  IMVEAVKPNLVDFFPVLKKMDPQGVRQRMSRHFDSLMKILYGLIDER---GGHVENNDVL 353
            IMVE  KPNLVD+FP+L+ MDPQG+++R++ +FD+L+K+   ++DER    G   + DVL
Sbjct: 206  IMVEIGKPNLVDYFPLLQWMDPQGIKRRLAYNFDTLIKLFDVMVDERLELNGPQNSRDVL 265

Query: 354  DELIKISQENPDEIDKIHIVHLIMDLFAAGTDTTSSTVEWGMAEVLKSSETT-AKAKAEL 530
            DEL+K+   N DEIDK HI H+ +DLF AGTDT+SSTVEW M+E+L+   T   KAKAEL
Sbjct: 266  DELLKLV--NTDEIDKSHIQHMFLDLFGAGTDTSSSTVEWAMSEILRKPATVLVKAKAEL 323

Query: 531  NQVLGKGKILEEADVSRLPYLRCIVTETLRLHPPVPFLLPRQTAEEAQLNGYKIPRNSQV 710
            +QV+G GKI+EEAD+S+L YLRCIV ETLRLHPP P L+PRQ  EE +L GY +P+NSQV
Sbjct: 324  DQVIGNGKIVEEADISKLDYLRCIVKETLRLHPPAPLLVPRQVQEEVELCGYTVPKNSQV 383

Query: 711  LVNAWAIGRDPDTWTNPLSFQPERFLDSEVDVRGKDFELIPFGAGRRICPGLSLAMRMVP 890
            LVNAWAIGRDP  W NPLSFQPERF+DSE+D+ G  +ELIPFGAGRRICPG+ LAMRMVP
Sbjct: 384  LVNAWAIGRDPMLWENPLSFQPERFVDSEIDINGHGYELIPFGAGRRICPGMPLAMRMVP 443

Query: 891  VMLGSLINCFDWELEGGTALSELDMDEKFGLTVQKLYPLCALATT 1025
            +MLGSL+NCFDW+L+GG A  +L+M++KFGLT+ KL+PL  + T+
Sbjct: 444  IMLGSLLNCFDWKLQGGIAPEDLNMEDKFGLTLAKLHPLRVVPTS 488


>gb|ADJ68324.1| geraniol 10-hydroxylase [Picrorhiza kurrooa]
          Length = 494

 Score =  446 bits (1147), Expect = e-123
 Identities = 215/342 (62%), Positives = 271/342 (79%), Gaps = 5/342 (1%)
 Frame = +3

Query: 6    KVNELIGYVKMCSENGEAVDIGRAVFRTSLNLLSNTIFSKDIADPYQDSAKEMRGLIWDI 185
            K  ELI Y +  S+ G A D+GRA FR  +NL     F K I DPY DSAKE + L+W+I
Sbjct: 149  KRQELIAYCRKRSQTGGAGDMGRAPFRNPVNLAVEHDFFKRIEDPYSDSAKEFKDLVWNI 208

Query: 186  MVEAVKPNLVDFFPVLKKMDPQGVRQRMSRHFDSLMKILYGLIDERG--GHVEN---NDV 350
            M+EA KPNLVDF+PVL+K+DPQG+R+RM+ HF  ++++  GLI+ER   G  ++    DV
Sbjct: 209  MLEAGKPNLVDFYPVLEKVDPQGIRKRMTVHFGKVIELFSGLINERTEKGKTQDAGTTDV 268

Query: 351  LDELIKISQENPDEIDKIHIVHLIMDLFAAGTDTTSSTVEWGMAEVLKSSETTAKAKAEL 530
            +D L+KIS+ENPDEID+ HI  L +DLF AGTDTTS+T+EW MAE LK+ E   KAKAEL
Sbjct: 269  IDVLLKISKENPDEIDRTHIERLCLDLFVAGTDTTSNTLEWAMAESLKNPEIMKKAKAEL 328

Query: 531  NQVLGKGKILEEADVSRLPYLRCIVTETLRLHPPVPFLLPRQTAEEAQLNGYKIPRNSQV 710
             QV+GKGK+L+EAD+ RLPYLRC+V ETLR+HPPVPFL+PR+  ++ ++ GY +P+NSQV
Sbjct: 329  EQVIGKGKVLDEADIQRLPYLRCMVKETLRIHPPVPFLIPRKVEQDVEVCGYTVPKNSQV 388

Query: 711  LVNAWAIGRDPDTWTNPLSFQPERFLDSEVDVRGKDFELIPFGAGRRICPGLSLAMRMVP 890
             VNAWAIGRD +TW NPL F+PERF++SEVD+RG+DFELIPFGAGRRICPGL LA+RMVP
Sbjct: 389  FVNAWAIGRDAETWPNPLEFKPERFMESEVDMRGRDFELIPFGAGRRICPGLPLALRMVP 448

Query: 891  VMLGSLINCFDWELEGGTALSELDMDEKFGLTVQKLYPLCAL 1016
            VMLGSL+N FDW+LEGG    ELDM+EKFG+T+QK  PL A+
Sbjct: 449  VMLGSLLNSFDWKLEGGAGPKELDMEEKFGITLQKALPLMAV 490