BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00005171
         (1246 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284951.1| PREDICTED: glutathione synthetase, chloropla...   521   e-145
emb|CBI34350.3| unnamed protein product [Vitis vinifera]              512   e-143
ref|XP_004157260.1| PREDICTED: glutathione synthetase, chloropla...   502   e-140
gb|ADM53349.1| glutathione synthetase [Cucumis sativus]               502   e-140
ref|XP_004135633.1| PREDICTED: glutathione synthetase, chloropla...   498   e-138

>ref|XP_002284951.1| PREDICTED: glutathione synthetase, chloroplastic-like [Vitis
            vinifera]
          Length = 507

 Score =  521 bits (1343), Expect = e-145
 Identities = 247/310 (79%), Positives = 285/310 (91%)
 Frame = -2

Query: 975  SLINTYKQHLGLDFKKVPSNIAVTQFADTLAKAWIEYNNPRAAILFVVQIEERNMYDQHW 796
            SL+N Y +HLGLD K++P N AVTQFA+ LA+AW EYNNPRA ++FVVQ EERNMYDQHW
Sbjct: 198  SLLNHYGKHLGLDSKRIPGNSAVTQFAEALAQAWKEYNNPRALVMFVVQTEERNMYDQHW 257

Query: 795  LSFVLNEKHNITTVRKTLAEIDAQGEIQPDGTLVIDGEAIAVVYFRAGYAPTDYPTESEW 616
            LS  + E++++TT+RKTLAEIDA+G++ PDGTL + G+ +AV+YFRAGYAPTDYP+ESEW
Sbjct: 258  LSTAMKERYDVTTIRKTLAEIDAEGKLLPDGTLTVGGQPVAVIYFRAGYAPTDYPSESEW 317

Query: 615  RARLLMEQSSAVKCPSISYHLVGTKKIQQELAKPEILERFLENKDDIAKLRKCFAGLWSL 436
            RARL+MEQSSAVKCPSISYHL GTKKIQQELAKP +LERFLENKDDIAKLRKCFAGLWSL
Sbjct: 318  RARLIMEQSSAVKCPSISYHLAGTKKIQQELAKPNVLERFLENKDDIAKLRKCFAGLWSL 377

Query: 435  EESNIVKDAIKRPELYVMKPQREGGGNNIYGDDVREALLRLQKEGTNEDAAYILMQRIFP 256
            ++SNI+K+AI+RPE++VMKPQREGGGNNIYGDDVRE LLRLQKEGT EDAAYILMQRIFP
Sbjct: 378  DDSNIIKEAIERPEVFVMKPQREGGGNNIYGDDVRETLLRLQKEGTEEDAAYILMQRIFP 437

Query: 255  VVAPTILMREGICHKDSAVSELGIYGAYLRNKDNVLINNQSGYLMRTKVSSSNEGGVAAG 76
             V+PT LMREGIC+KD A+SELGIYGAYLRNK+ V+IN+Q G+LMRTKVSSSNEGGVAAG
Sbjct: 438  NVSPTFLMREGICYKDHAISELGIYGAYLRNKEKVIINDQCGHLMRTKVSSSNEGGVAAG 497

Query: 75   FAVLDSIYLT 46
            FAVLDSIYLT
Sbjct: 498  FAVLDSIYLT 507


>emb|CBI34350.3| unnamed protein product [Vitis vinifera]
          Length = 477

 Score =  512 bits (1319), Expect = e-143
 Identities = 248/329 (75%), Positives = 285/329 (86%), Gaps = 19/329 (5%)
 Frame = -2

Query: 975  SLINTYKQHLGLDFKKVPSNIAVTQFADTLAKAWIEYNNPRAAILFVVQIEERNMYDQHW 796
            SL+N Y +HLGLD K++P N AVTQFA+ LA+AW EYNNPRA ++FVVQ EERNMYDQHW
Sbjct: 149  SLLNHYGKHLGLDSKRIPGNSAVTQFAEALAQAWKEYNNPRALVMFVVQTEERNMYDQHW 208

Query: 795  LSFVLNEK-------------------HNITTVRKTLAEIDAQGEIQPDGTLVIDGEAIA 673
            LS  + E+                   H++TT+RKTLAEIDA+G++ PDGTL + G+ +A
Sbjct: 209  LSTAMKERYPFESFQRAPMFFPQLYNTHDVTTIRKTLAEIDAEGKLLPDGTLTVGGQPVA 268

Query: 672  VVYFRAGYAPTDYPTESEWRARLLMEQSSAVKCPSISYHLVGTKKIQQELAKPEILERFL 493
            V+YFRAGYAPTDYP+ESEWRARL+MEQSSAVKCPSISYHL GTKKIQQELAKP +LERFL
Sbjct: 269  VIYFRAGYAPTDYPSESEWRARLIMEQSSAVKCPSISYHLAGTKKIQQELAKPNVLERFL 328

Query: 492  ENKDDIAKLRKCFAGLWSLEESNIVKDAIKRPELYVMKPQREGGGNNIYGDDVREALLRL 313
            ENKDDIAKLRKCFAGLWSL++SNI+K+AI+RPE++VMKPQREGGGNNIYGDDVRE LLRL
Sbjct: 329  ENKDDIAKLRKCFAGLWSLDDSNIIKEAIERPEVFVMKPQREGGGNNIYGDDVRETLLRL 388

Query: 312  QKEGTNEDAAYILMQRIFPVVAPTILMREGICHKDSAVSELGIYGAYLRNKDNVLINNQS 133
            QKEGT EDAAYILMQRIFP V+PT LMREGIC+KD A+SELGIYGAYLRNK+ V+IN+Q 
Sbjct: 389  QKEGTEEDAAYILMQRIFPNVSPTFLMREGICYKDHAISELGIYGAYLRNKEKVIINDQC 448

Query: 132  GYLMRTKVSSSNEGGVAAGFAVLDSIYLT 46
            G+LMRTKVSSSNEGGVAAGFAVLDSIYLT
Sbjct: 449  GHLMRTKVSSSNEGGVAAGFAVLDSIYLT 477


>ref|XP_004157260.1| PREDICTED: glutathione synthetase, chloroplastic-like [Cucumis
            sativus]
          Length = 549

 Score =  502 bits (1292), Expect = e-140
 Identities = 241/310 (77%), Positives = 276/310 (89%)
 Frame = -2

Query: 975  SLINTYKQHLGLDFKKVPSNIAVTQFADTLAKAWIEYNNPRAAILFVVQIEERNMYDQHW 796
            +L+  Y + LGLD++ VP N A  QFAD LAKAWIEYN+P A ILFVVQ EERNMYDQHW
Sbjct: 240  ALLGEYGKVLGLDYRNVPENYAANQFADVLAKAWIEYNDPSAVILFVVQAEERNMYDQHW 299

Query: 795  LSFVLNEKHNITTVRKTLAEIDAQGEIQPDGTLVIDGEAIAVVYFRAGYAPTDYPTESEW 616
            LS  L ++HN+ T+RKTLAEID +G ++ DG L++DG+AIAVVYFRAGY P DYP+ESEW
Sbjct: 300  LSVNLRKRHNVKTLRKTLAEIDREGVLRSDGKLIVDGQAIAVVYFRAGYTPRDYPSESEW 359

Query: 615  RARLLMEQSSAVKCPSISYHLVGTKKIQQELAKPEILERFLENKDDIAKLRKCFAGLWSL 436
            RARLLMEQSSAVKCPSISYHLVGTKKIQQELA+P +LERFL+++DDIAK+RKCFAGLWSL
Sbjct: 360  RARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEDDIAKIRKCFAGLWSL 419

Query: 435  EESNIVKDAIKRPELYVMKPQREGGGNNIYGDDVREALLRLQKEGTNEDAAYILMQRIFP 256
            ++ +IVK AI+ PEL+VMKPQREGGGNNIYGDDVREALLRLQKEG+ ED AYILMQRIFP
Sbjct: 420  DDQDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLRLQKEGSEEDGAYILMQRIFP 479

Query: 255  VVAPTILMREGICHKDSAVSELGIYGAYLRNKDNVLINNQSGYLMRTKVSSSNEGGVAAG 76
             V+PTI +R+GICHKD AVSELGI+GAYLRNKD V++N QSGYLMRTKVSSSNEGGVAAG
Sbjct: 480  SVSPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNEQSGYLMRTKVSSSNEGGVAAG 539

Query: 75   FAVLDSIYLT 46
            FAVLDSIYLT
Sbjct: 540  FAVLDSIYLT 549


>gb|ADM53349.1| glutathione synthetase [Cucumis sativus]
          Length = 472

 Score =  502 bits (1292), Expect = e-140
 Identities = 241/310 (77%), Positives = 276/310 (89%)
 Frame = -2

Query: 975  SLINTYKQHLGLDFKKVPSNIAVTQFADTLAKAWIEYNNPRAAILFVVQIEERNMYDQHW 796
            +L+  Y + LGLD++ VP N A  QFAD LAKAWIEYN+P A ILFVVQ EERNMYDQHW
Sbjct: 163  ALLGEYGKVLGLDYRNVPENYAANQFADVLAKAWIEYNDPSAVILFVVQAEERNMYDQHW 222

Query: 795  LSFVLNEKHNITTVRKTLAEIDAQGEIQPDGTLVIDGEAIAVVYFRAGYAPTDYPTESEW 616
            LS  L ++HN+ T+RKTLAEID +G ++ DG L++DG+AIAVVYFRAGY P DYP+ESEW
Sbjct: 223  LSVNLRKRHNVKTLRKTLAEIDREGVLRSDGKLIVDGQAIAVVYFRAGYTPRDYPSESEW 282

Query: 615  RARLLMEQSSAVKCPSISYHLVGTKKIQQELAKPEILERFLENKDDIAKLRKCFAGLWSL 436
            RARLLMEQSSAVKCPSISYHLVGTKKIQQELA+P +LERFL+++DDIAK+RKCFAGLWSL
Sbjct: 283  RARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEDDIAKIRKCFAGLWSL 342

Query: 435  EESNIVKDAIKRPELYVMKPQREGGGNNIYGDDVREALLRLQKEGTNEDAAYILMQRIFP 256
            ++ +IVK AI+ PEL+VMKPQREGGGNNIYGDDVREALLRLQKEG+ ED AYILMQRIFP
Sbjct: 343  DDQDIVKKAIETPELFVMKPQREGGGNNIYGDDVREALLRLQKEGSEEDGAYILMQRIFP 402

Query: 255  VVAPTILMREGICHKDSAVSELGIYGAYLRNKDNVLINNQSGYLMRTKVSSSNEGGVAAG 76
             V+PTI +R+GICHKD AVSELGI+GAYLRNKD V++N QSGYLMRTKVSSSNEGGVAAG
Sbjct: 403  SVSPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNEQSGYLMRTKVSSSNEGGVAAG 462

Query: 75   FAVLDSIYLT 46
            FAVLDSIYLT
Sbjct: 463  FAVLDSIYLT 472


>ref|XP_004135633.1| PREDICTED: glutathione synthetase, chloroplastic-like [Cucumis
            sativus]
          Length = 547

 Score =  498 bits (1282), Expect = e-138
 Identities = 239/310 (77%), Positives = 274/310 (88%)
 Frame = -2

Query: 975  SLINTYKQHLGLDFKKVPSNIAVTQFADTLAKAWIEYNNPRAAILFVVQIEERNMYDQHW 796
            +L+  Y + LGLD++ VP N A   FAD LAKAWIEYN+P A ILFVVQ EERNMYDQHW
Sbjct: 238  ALLGEYGKVLGLDYRNVPENYAANHFADVLAKAWIEYNDPSAVILFVVQAEERNMYDQHW 297

Query: 795  LSFVLNEKHNITTVRKTLAEIDAQGEIQPDGTLVIDGEAIAVVYFRAGYAPTDYPTESEW 616
            LS  L ++HN+ T+RKTLAEID +G ++ DG L++DG+AIAVVYFRAGY P DYP+ESEW
Sbjct: 298  LSVNLRKRHNVKTLRKTLAEIDREGVLRSDGKLIVDGQAIAVVYFRAGYTPRDYPSESEW 357

Query: 615  RARLLMEQSSAVKCPSISYHLVGTKKIQQELAKPEILERFLENKDDIAKLRKCFAGLWSL 436
            RARLLMEQSSAVKCPSISYHLVGTKKIQQELA+P +LERFL+++DDIAK+RKCFAGLWSL
Sbjct: 358  RARLLMEQSSAVKCPSISYHLVGTKKIQQELARPNVLERFLDSEDDIAKIRKCFAGLWSL 417

Query: 435  EESNIVKDAIKRPELYVMKPQREGGGNNIYGDDVREALLRLQKEGTNEDAAYILMQRIFP 256
            ++ +IV  AI+ PEL+VMKPQREGGGNNIYGDDVREALLRLQKEG+ ED AYILMQRIFP
Sbjct: 418  DDQDIVNKAIETPELFVMKPQREGGGNNIYGDDVREALLRLQKEGSEEDGAYILMQRIFP 477

Query: 255  VVAPTILMREGICHKDSAVSELGIYGAYLRNKDNVLINNQSGYLMRTKVSSSNEGGVAAG 76
             V+PTI +R+GICHKD AVSELGI+GAYLRNKD V++N QSGYLMRTKVSSSNEGGVAAG
Sbjct: 478  SVSPTIFVRDGICHKDHAVSELGIFGAYLRNKDKVIVNEQSGYLMRTKVSSSNEGGVAAG 537

Query: 75   FAVLDSIYLT 46
            FAVLDSIYLT
Sbjct: 538  FAVLDSIYLT 547