BLASTX nr result
ID: Bupleurum21_contig00004891
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00004891
(1521 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ABB60086.1| brassinosteroid-6-oxidase [Vitis vinifera] 794 0.0
ref|XP_002281338.1| PREDICTED: cytochrome P450 85A [Vitis vinife... 794 0.0
gb|ABG36709.1| cytochrome P450 CYP85A1 [Nicotiana tabacum] 788 0.0
emb|CAN66537.1| hypothetical protein VITISV_029635 [Vitis vinifera] 777 0.0
gb|ACR20476.1| steroid C-6 oxidase [Gossypium hirsutum] 772 0.0
>gb|ABB60086.1| brassinosteroid-6-oxidase [Vitis vinifera]
Length = 460
Score = 794 bits (2051), Expect = 0.0
Identities = 385/466 (82%), Positives = 421/466 (90%)
Frame = -1
Query: 1521 LCLISTALLRWNEVKYRKKGLPPGTMGWPVFGETTEFLKQGPAFMKNQRARFGNFFKSHI 1342
LC I TALLRWNEV+YRKKGLPPGTMGWPVFGETTEFLKQGP+FMKNQRAR+G FFKSH+
Sbjct: 11 LC-ICTALLRWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPSFMKNQRARYGKFFKSHL 69
Query: 1341 LGCPTIVSMDPELNKYILMNESKGLVPGYPQSMLDILGKCNIAAVHGSTHKNMRGALLAL 1162
LGCPT VSMDPELN+YILMNE+KGLVPGYPQSMLDILGKCNIAAVHGSTHK MRGALLAL
Sbjct: 70 LGCPTTVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLAL 129
Query: 1161 VSPPMIRDQLLPKIDHFMRSHLTNWNDKIIDIQEKTKEMAFMSSLKQIAGVESSSMCQEF 982
+SP MIR QLLPKID FMRSHL W+ KII+IQEKTKEMA +SSLKQIAG+ES ++ +EF
Sbjct: 130 ISPTMIRGQLLPKIDEFMRSHLNKWDTKIINIQEKTKEMALLSSLKQIAGIESGTISKEF 189
Query: 981 MPEFFKLVVGTLSLPINLPNTNYGRGFQARQSIVSMLRKLIEKRRDSHETHNDMLAHLMN 802
MPEFFKLV+GT+SLPI+LP TNY RGFQAR++IV MLR+LIE+R+ S ETHNDML LM
Sbjct: 190 MPEFFKLVLGTISLPIDLPGTNYRRGFQARKNIVGMLRQLIEERKASQETHNDMLGCLMR 249
Query: 801 TEENKYKLSDEEIIDQIITILYSGYETVSTTSMMAVKYLHDHPTVLQELRKEHLAIREKK 622
T EN+YKLSDEEIID IITILYSGYETVSTTSMMAVKYLHDHP VL ELRKEHLAIRE+K
Sbjct: 250 TNENRYKLSDEEIIDLIITILYSGYETVSTTSMMAVKYLHDHPRVLDELRKEHLAIRERK 309
Query: 621 RPEDPINWNDYKSMRFTRAVIFETSRLATIVNGVLRKTTKEMEINGYVIPEGWRIYVYTR 442
RPEDPI+WNDYK MRFTRAVIFETSRLATIVNGVLRKTTK+ME+NG+VIP+GWRIYVYTR
Sbjct: 310 RPEDPIDWNDYKLMRFTRAVIFETSRLATIVNGVLRKTTKDMELNGFVIPKGWRIYVYTR 369
Query: 441 EINYDPRLYPEPLAFNPWRWLXXXXXXXXXXXXXXXDKSMDAQNYFFIFGGGTRQCPGKE 262
EINYDP LYP+PLAFNPWRWL DKS+++QNYF +FGGGTRQCPGKE
Sbjct: 370 EINYDPLLYPDPLAFNPWRWL---------------DKSLESQNYFLLFGGGTRQCPGKE 414
Query: 261 LGVAEISTFLHYFVTKYRWEEVGGDKLMKFPRVEAPNGLHIRVSDY 124
LG+AEISTFLHYFVT+YRWEEVGGDKLMKFPRVEAPNGLHIRVS Y
Sbjct: 415 LGIAEISTFLHYFVTRYRWEEVGGDKLMKFPRVEAPNGLHIRVSAY 460
>ref|XP_002281338.1| PREDICTED: cytochrome P450 85A [Vitis vinifera]
gi|298204461|emb|CBI16941.3| unnamed protein product
[Vitis vinifera]
Length = 464
Score = 794 bits (2051), Expect = 0.0
Identities = 385/466 (82%), Positives = 421/466 (90%)
Frame = -1
Query: 1521 LCLISTALLRWNEVKYRKKGLPPGTMGWPVFGETTEFLKQGPAFMKNQRARFGNFFKSHI 1342
LC I TALLRWNEV+YRKKGLPPGTMGWPVFGETTEFLKQGP+FMKNQRAR+G FFKSH+
Sbjct: 15 LC-ICTALLRWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPSFMKNQRARYGKFFKSHL 73
Query: 1341 LGCPTIVSMDPELNKYILMNESKGLVPGYPQSMLDILGKCNIAAVHGSTHKNMRGALLAL 1162
LGCPT VSMDPELN+YILMNE+KGLVPGYPQSMLDILGKCNIAAVHGSTHK MRGALLAL
Sbjct: 74 LGCPTTVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLAL 133
Query: 1161 VSPPMIRDQLLPKIDHFMRSHLTNWNDKIIDIQEKTKEMAFMSSLKQIAGVESSSMCQEF 982
+SP MIR QLLPKID FMRSHL W+ KII+IQEKTKEMA +SSLKQIAG+ES ++ +EF
Sbjct: 134 ISPTMIRGQLLPKIDEFMRSHLNKWDTKIINIQEKTKEMALLSSLKQIAGIESGTISKEF 193
Query: 981 MPEFFKLVVGTLSLPINLPNTNYGRGFQARQSIVSMLRKLIEKRRDSHETHNDMLAHLMN 802
MPEFFKLV+GT+SLPI+LP TNY RGFQAR++IV MLR+LIE+R+ S ETHNDML LM
Sbjct: 194 MPEFFKLVLGTISLPIDLPGTNYRRGFQARKNIVGMLRQLIEERKASQETHNDMLGCLMR 253
Query: 801 TEENKYKLSDEEIIDQIITILYSGYETVSTTSMMAVKYLHDHPTVLQELRKEHLAIREKK 622
T EN+YKLSDEEIID IITILYSGYETVSTTSMMAVKYLHDHP VL ELRKEHLAIRE+K
Sbjct: 254 TNENRYKLSDEEIIDLIITILYSGYETVSTTSMMAVKYLHDHPRVLDELRKEHLAIRERK 313
Query: 621 RPEDPINWNDYKSMRFTRAVIFETSRLATIVNGVLRKTTKEMEINGYVIPEGWRIYVYTR 442
RPEDPI+WNDYK MRFTRAVIFETSRLATIVNGVLRKTTK+ME+NG+VIP+GWRIYVYTR
Sbjct: 314 RPEDPIDWNDYKLMRFTRAVIFETSRLATIVNGVLRKTTKDMELNGFVIPKGWRIYVYTR 373
Query: 441 EINYDPRLYPEPLAFNPWRWLXXXXXXXXXXXXXXXDKSMDAQNYFFIFGGGTRQCPGKE 262
EINYDP LYP+PLAFNPWRWL DKS+++QNYF +FGGGTRQCPGKE
Sbjct: 374 EINYDPLLYPDPLAFNPWRWL---------------DKSLESQNYFLLFGGGTRQCPGKE 418
Query: 261 LGVAEISTFLHYFVTKYRWEEVGGDKLMKFPRVEAPNGLHIRVSDY 124
LG+AEISTFLHYFVT+YRWEEVGGDKLMKFPRVEAPNGLHIRVS Y
Sbjct: 419 LGIAEISTFLHYFVTRYRWEEVGGDKLMKFPRVEAPNGLHIRVSAY 464
>gb|ABG36709.1| cytochrome P450 CYP85A1 [Nicotiana tabacum]
Length = 464
Score = 788 bits (2036), Expect = 0.0
Identities = 379/466 (81%), Positives = 421/466 (90%)
Frame = -1
Query: 1521 LCLISTALLRWNEVKYRKKGLPPGTMGWPVFGETTEFLKQGPAFMKNQRARFGNFFKSHI 1342
LC+ STALLRWN+VKY KK LPPGTMGWP+FGETTEFLKQGP+FMKNQRARFG+FFKSHI
Sbjct: 14 LCIFSTALLRWNQVKYNKKSLPPGTMGWPLFGETTEFLKQGPSFMKNQRARFGSFFKSHI 73
Query: 1341 LGCPTIVSMDPELNKYILMNESKGLVPGYPQSMLDILGKCNIAAVHGSTHKNMRGALLAL 1162
LGCPTIVSMD ELN+YIL+NE+KGLVPGYPQSMLDILGKCNIAAVHGS HK MRGALL+L
Sbjct: 74 LGCPTIVSMDSELNRYILVNEAKGLVPGYPQSMLDILGKCNIAAVHGSAHKYMRGALLSL 133
Query: 1161 VSPPMIRDQLLPKIDHFMRSHLTNWNDKIIDIQEKTKEMAFMSSLKQIAGVESSSMCQEF 982
+SP MIRDQLLPKID FMRSHLTNW+ K+IDIQEKT +MAF+SSLKQIAG+ES+S+ QEF
Sbjct: 134 ISPTMIRDQLLPKIDEFMRSHLTNWDSKVIDIQEKTNKMAFLSSLKQIAGIESTSLAQEF 193
Query: 981 MPEFFKLVVGTLSLPINLPNTNYGRGFQARQSIVSMLRKLIEKRRDSHETHNDMLAHLMN 802
MPEFFKLV+GTLSLPINLPNTNYGRG QAR+ IVS+LR LIE+RR S E +DML +LMN
Sbjct: 194 MPEFFKLVLGTLSLPINLPNTNYGRGLQARKIIVSLLRTLIEERRASKEIQHDMLGYLMN 253
Query: 801 TEENKYKLSDEEIIDQIITILYSGYETVSTTSMMAVKYLHDHPTVLQELRKEHLAIREKK 622
E N+YKL+D+E+ID IITILYSGYETVSTTSMMAVKYLHDHP VL+ELRKEHLAIREKK
Sbjct: 254 EEANRYKLTDDEMIDLIITILYSGYETVSTTSMMAVKYLHDHPKVLEELRKEHLAIREKK 313
Query: 621 RPEDPINWNDYKSMRFTRAVIFETSRLATIVNGVLRKTTKEMEINGYVIPEGWRIYVYTR 442
+PEDPI++NDYK+MRFTRAVI ETSRLATIVNGVLRKTT++MEINGY+IP+GWRIYVYTR
Sbjct: 314 KPEDPIDYNDYKAMRFTRAVILETSRLATIVNGVLRKTTQDMEINGYIIPKGWRIYVYTR 373
Query: 441 EINYDPRLYPEPLAFNPWRWLXXXXXXXXXXXXXXXDKSMDAQNYFFIFGGGTRQCPGKE 262
E+NYDPRLYP+P AFNPWRWL DKS++ QN F +FGGGTRQCPGKE
Sbjct: 374 ELNYDPRLYPDPYAFNPWRWL---------------DKSLENQNSFLVFGGGTRQCPGKE 418
Query: 261 LGVAEISTFLHYFVTKYRWEEVGGDKLMKFPRVEAPNGLHIRVSDY 124
LGVAEISTFLHYFVTKY+WEEVGGDKLMKFPRVEAPNGL IRVS Y
Sbjct: 419 LGVAEISTFLHYFVTKYKWEEVGGDKLMKFPRVEAPNGLRIRVSTY 464
>emb|CAN66537.1| hypothetical protein VITISV_029635 [Vitis vinifera]
Length = 463
Score = 777 bits (2006), Expect = 0.0
Identities = 378/469 (80%), Positives = 416/469 (88%), Gaps = 3/469 (0%)
Frame = -1
Query: 1521 LCLISTALLRWNEVKYRKKGLPPGTMGWPVFGETTEFLKQGPAFMKNQRARFGNFFKSHI 1342
LC I TALLRWNEV+YRKKGLPPGTMGWPVFGETTEFLKQGP+FMKNQRAR+G FFKSH+
Sbjct: 11 LC-ICTALLRWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPSFMKNQRARYGKFFKSHL 69
Query: 1341 LGCPTIVSMDPELNKYILMNESKGLVPGYPQSMLDILGKCNIAAVHGSTHKNMRGALLAL 1162
LGCPT VSMDPELN+YILMNE+KGLVPGYPQSMLDILGKCNIAAVHGSTHK MRGALLAL
Sbjct: 70 LGCPTTVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKYMRGALLAL 129
Query: 1161 VSPPMIRDQLLPKIDHFMRSHLTNWNDKIIDIQEKTKEM---AFMSSLKQIAGVESSSMC 991
+SP MIR QLLPKID FMRSHL W+ KII+IQEKTKE+ + S +AG+ES ++
Sbjct: 130 ISPTMIRGQLLPKIDEFMRSHLNKWDTKIINIQEKTKEVYGTSLFSQADAVAGIESGTIS 189
Query: 990 QEFMPEFFKLVVGTLSLPINLPNTNYGRGFQARQSIVSMLRKLIEKRRDSHETHNDMLAH 811
+EFMPEFFKLV+GT+SLPI+LP TNY RGFQAR++IV MLR+LIE+R+ S ETHNDML
Sbjct: 190 KEFMPEFFKLVLGTISLPIDLPGTNYRRGFQARKNIVGMLRQLIEERKASQETHNDMLGC 249
Query: 810 LMNTEENKYKLSDEEIIDQIITILYSGYETVSTTSMMAVKYLHDHPTVLQELRKEHLAIR 631
LM T EN+YKLSDEEIID IITILYSGYETVSTTSMMAVKYLHDHP VL ELRKEHLAIR
Sbjct: 250 LMRTNENRYKLSDEEIIDLIITILYSGYETVSTTSMMAVKYLHDHPRVLDELRKEHLAIR 309
Query: 630 EKKRPEDPINWNDYKSMRFTRAVIFETSRLATIVNGVLRKTTKEMEINGYVIPEGWRIYV 451
E+KRPEDPI+WNDYK MRFTRAVIFETSRLATIVNGVLRKTTK+ME+NG+VIP+GWRIYV
Sbjct: 310 ERKRPEDPIDWNDYKLMRFTRAVIFETSRLATIVNGVLRKTTKDMELNGFVIPKGWRIYV 369
Query: 450 YTREINYDPRLYPEPLAFNPWRWLXXXXXXXXXXXXXXXDKSMDAQNYFFIFGGGTRQCP 271
YTREINYDP LYP+PLAFNPWRWL DKS+++QNYF +FGGGTRQCP
Sbjct: 370 YTREINYDPLLYPDPLAFNPWRWL---------------DKSLESQNYFLLFGGGTRQCP 414
Query: 270 GKELGVAEISTFLHYFVTKYRWEEVGGDKLMKFPRVEAPNGLHIRVSDY 124
GKELG+AEISTFLHYFVT+YRWEEVGGDKLMKFPRVEAPNGLHIRVS Y
Sbjct: 415 GKELGIAEISTFLHYFVTRYRWEEVGGDKLMKFPRVEAPNGLHIRVSAY 463
>gb|ACR20476.1| steroid C-6 oxidase [Gossypium hirsutum]
Length = 465
Score = 772 bits (1994), Expect = 0.0
Identities = 371/467 (79%), Positives = 421/467 (90%), Gaps = 1/467 (0%)
Frame = -1
Query: 1521 LCLISTALLRWNEVKYRKKGLPPGTMGWPVFGETTEFLKQGPAFMKNQRARFGNFFKSHI 1342
LC+ TA+LRWNE++YRKKGLPPGTMGWPVFGETTEFLKQGP FMKNQRAR+G+FFKSHI
Sbjct: 15 LCMC-TAVLRWNEMRYRKKGLPPGTMGWPVFGETTEFLKQGPNFMKNQRARYGSFFKSHI 73
Query: 1341 LGCPTIVSMDPELNKYILMNESKGLVPGYPQSMLDILGKCNIAAVHGSTHKNMRGALLAL 1162
LGCPT+VSMDPELN+YILMNE+KGLVPGYPQSMLDILGKCNIAAVHGSTHK MRGALLAL
Sbjct: 74 LGCPTVVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHGSTHKQMRGALLAL 133
Query: 1161 VSPPMIRDQLLPKIDHFMRSHLTNWNDKIIDIQEKTKEMAFMSSLKQIAGVESSSMCQEF 982
+SP MIR QLLPKID FMR+ L+NW++++IDIQ++TKEMAF+SSLKQIA ESSS+ Q+F
Sbjct: 134 ISPTMIRQQLLPKIDEFMRAFLSNWDNQVIDIQDRTKEMAFLSSLKQIASAESSSVAQQF 193
Query: 981 MPEFFKLVVGTLSLPINLPNTNYGRGFQARQSIVSMLRKLIEKRRDSHETHNDMLAHLM- 805
MPEFFKLV+GTLSL I+LP TNY RGFQARQ+I+ +L +LIEKRRDS E+H DML +LM
Sbjct: 194 MPEFFKLVLGTLSLHIDLPGTNYRRGFQARQNILKILGQLIEKRRDSEESHKDMLGYLMR 253
Query: 804 NTEENKYKLSDEEIIDQIITILYSGYETVSTTSMMAVKYLHDHPTVLQELRKEHLAIREK 625
N + N++KLSD+EIIDQIITILYSGYETVSTTSMMAVKYLHDHPTVL+ELRKEH+ IRE+
Sbjct: 254 NDDNNRHKLSDDEIIDQIITILYSGYETVSTTSMMAVKYLHDHPTVLEELRKEHMGIRER 313
Query: 624 KRPEDPINWNDYKSMRFTRAVIFETSRLATIVNGVLRKTTKEMEINGYVIPEGWRIYVYT 445
KR ++PI WND KSMRFTRAVIFETSRLATIVNGVLRKTT++ME+NGYVIP+GWRIYVYT
Sbjct: 314 KRADEPIEWNDLKSMRFTRAVIFETSRLATIVNGVLRKTTQDMELNGYVIPKGWRIYVYT 373
Query: 444 REINYDPRLYPEPLAFNPWRWLXXXXXXXXXXXXXXXDKSMDAQNYFFIFGGGTRQCPGK 265
REINYDP LYP+PLAFNPWRW+ K +++QNYF IFGGGTRQCPGK
Sbjct: 374 REINYDPFLYPDPLAFNPWRWMV---------------KGLESQNYFLIFGGGTRQCPGK 418
Query: 264 ELGVAEISTFLHYFVTKYRWEEVGGDKLMKFPRVEAPNGLHIRVSDY 124
ELG+AEISTFLHYFVT+YRWEEVGG+KLMKFPRVEAPNGLHIRVS Y
Sbjct: 419 ELGIAEISTFLHYFVTRYRWEEVGGEKLMKFPRVEAPNGLHIRVSSY 465