BLASTX nr result
ID: Bupleurum21_contig00004856
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00004856
(1534 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002285843.1| PREDICTED: aminoacylase-1 isoform 1 [Vitis v... 612 e-173
ref|XP_002300996.1| predicted protein [Populus trichocarpa] gi|2... 606 e-171
ref|NP_175103.2| aminoacylase [Arabidopsis thaliana] gi|8655992|... 597 e-168
dbj|BAE99837.1| aminoacylase like protein [Arabidopsis thaliana] 597 e-168
ref|XP_003634462.1| PREDICTED: aminoacylase-1 isoform 3 [Vitis v... 597 e-168
>ref|XP_002285843.1| PREDICTED: aminoacylase-1 isoform 1 [Vitis vinifera]
gi|359492737|ref|XP_003634461.1| PREDICTED:
aminoacylase-1 isoform 2 [Vitis vinifera]
Length = 450
Score = 612 bits (1578), Expect = e-173
Identities = 298/420 (70%), Positives = 352/420 (83%)
Frame = -2
Query: 1329 EYNDNQECEDTPITRFQQYLRIKTAHPTPDYTPAIKFLTHQAQSIPNLQIRTLDFSSSPN 1150
+++ +E ED+PITRFQ YLRI T HP PDY A+ FL QA I LQ +TL+F+ S
Sbjct: 33 QHHLEKEEEDSPITRFQGYLRINTVHPNPDYASAVSFLKSQALYI-GLQTKTLEFARS-- 89
Query: 1149 YTKPLLLLTLPGSNPTLSSILFNSHIDSVPAEPEKWTHHPFAATLTAEGKIFARGAQDDK 970
KPLLLLT PGS+P+L SIL NSH+DSVP EP KW H PF+A + +GKIFARG+QDDK
Sbjct: 90 --KPLLLLTWPGSDPSLPSILLNSHLDSVPVEPSKWLHPPFSAYRSPDGKIFARGSQDDK 147
Query: 969 CIGMQYLETLRNIQKDSNFKMPVRTIHVSFVPDEEIGGFDGMMKFVESQEFKELNVGFVM 790
CI MQYLE +RN++ NF+ P RTIH+S+VPDEEIGGFDG KFV S+EF +LNVGF++
Sbjct: 148 CIAMQYLEAIRNLRAQ-NFQ-PTRTIHISYVPDEEIGGFDGAAKFVASKEFADLNVGFML 205
Query: 789 DEGQTSTNDEFRVFYADRAPWNLVIKAHGTPGHGSKLFDNSAMENLMRSVELITKFRENQ 610
DEGQ ST DEFRVFYADR+PWNL+IKA G PGHGS+L+DNSAMENLM+SVE+ITKFRE+
Sbjct: 206 DEGQASTGDEFRVFYADRSPWNLIIKAFGMPGHGSRLYDNSAMENLMKSVEIITKFRESL 265
Query: 609 FDLVKAGLKLTSEVISANCVYLKAGTPSPTGFVMNMQPSEAEAGFDVRLPPTADSDMLRR 430
FD+VKAG SEVIS N VYLKAG PSPTGFVMNMQPSEAEAGFD+R+PPTAD D+++
Sbjct: 266 FDVVKAGKAANSEVISVNPVYLKAGIPSPTGFVMNMQPSEAEAGFDLRMPPTADPDLVKI 325
Query: 429 RIAEEWAPAIRNMTYKITQKGPLKDYAGRPLMTATNNSNPWWSVFKQTVIAAGGKLAKPE 250
RIAEEWAPAIRNMTY+I +KGP++DY GRPLMT TN+SNPWWS+FKQ + AGGKLAKPE
Sbjct: 326 RIAEEWAPAIRNMTYQIIEKGPIRDYMGRPLMTLTNDSNPWWSIFKQAITEAGGKLAKPE 385
Query: 249 ILASTTDARFMRQIGIPTLGFSPMKNTPILLHDHNEYLEASVYLEGIKVYESIFKALSSF 70
ILASTTDAR+MRQ+GIPTLGFSPM NTPILLHDHNE+L+ ++YL GIKVYES+ +LSSF
Sbjct: 386 ILASTTDARYMRQMGIPTLGFSPMTNTPILLHDHNEFLKDTIYLRGIKVYESVISSLSSF 445
>ref|XP_002300996.1| predicted protein [Populus trichocarpa] gi|222842722|gb|EEE80269.1|
predicted protein [Populus trichocarpa]
Length = 442
Score = 606 bits (1563), Expect = e-171
Identities = 292/416 (70%), Positives = 351/416 (84%)
Frame = -2
Query: 1314 QECEDTPITRFQQYLRIKTAHPTPDYTPAIKFLTHQAQSIPNLQIRTLDFSSSPNYTKPL 1135
Q+ +TP+ RFQ+YLR TAHP P+YT + FLT AQS+ L+ +TL+F S+ KP
Sbjct: 29 QQDPETPVARFQKYLRFNTAHPNPNYTAPVSFLTSLAQSL-GLKTQTLEFISN----KPT 83
Query: 1134 LLLTLPGSNPTLSSILFNSHIDSVPAEPEKWTHHPFAATLTAEGKIFARGAQDDKCIGMQ 955
LL+T GSNP+L S+LFNSH+DSVPAEP KWTH PF+ATLT EGKIFARGAQDDKCI +Q
Sbjct: 84 LLITWQGSNPSLPSLLFNSHLDSVPAEPSKWTHPPFSATLTPEGKIFARGAQDDKCIAIQ 143
Query: 954 YLETLRNIQKDSNFKMPVRTIHVSFVPDEEIGGFDGMMKFVESQEFKELNVGFVMDEGQT 775
YLE +RN+ K NF +P RT+H+SFVPDEEIGG DG KFV+S+EFK+L+VGFV+DEG
Sbjct: 144 YLEAIRNL-KARNF-VPTRTLHISFVPDEEIGGIDGADKFVKSKEFKDLDVGFVLDEGLA 201
Query: 774 STNDEFRVFYADRAPWNLVIKAHGTPGHGSKLFDNSAMENLMRSVELITKFRENQFDLVK 595
S NDEFRVFYADR+PWNL+IKA G PGHGS+++DN AMENLM S+E+I +FR++QFD+VK
Sbjct: 202 SVNDEFRVFYADRSPWNLIIKAKGVPGHGSRMYDNGAMENLMDSIEVINRFRDSQFDIVK 261
Query: 594 AGLKLTSEVISANCVYLKAGTPSPTGFVMNMQPSEAEAGFDVRLPPTADSDMLRRRIAEE 415
AG SEVIS N V+LKAG PSPTGFVMNMQPSEAEAGFD+RLPPTAD D +++RIAEE
Sbjct: 262 AGKASNSEVISVNPVFLKAGIPSPTGFVMNMQPSEAEAGFDLRLPPTADPDPMKKRIAEE 321
Query: 414 WAPAIRNMTYKITQKGPLKDYAGRPLMTATNNSNPWWSVFKQTVIAAGGKLAKPEILAST 235
WAPA+RNMTY+I +KGPL+DY GRPLMTAT++SNPWWSVFKQ + AAGGKLAKPEIL ST
Sbjct: 322 WAPAVRNMTYEIIEKGPLRDYMGRPLMTATDDSNPWWSVFKQAIAAAGGKLAKPEILRST 381
Query: 234 TDARFMRQIGIPTLGFSPMKNTPILLHDHNEYLEASVYLEGIKVYESIFKALSSFE 67
TDARFMRQ+GIPT GFSPM NTP+L HDHNE+L+ +++L+GI+VYE I ALSSFE
Sbjct: 382 TDARFMRQLGIPTFGFSPMTNTPVLSHDHNEFLKDTIFLKGIEVYEHIIHALSSFE 437
>ref|NP_175103.2| aminoacylase [Arabidopsis thaliana]
gi|8655992|gb|AAF78265.1|AC020576_9 Contains similarity
to aminoacylase from Sus scrofa domestica gi|S27010 and
contains a peptidase M20 PF|01546 domain. ESTs gb|H76043,
gb|AA394953, gb|AI995115, gb|AA651481 come from this gene
[Arabidopsis thaliana] gi|109946615|gb|ABG48486.1|
At1g44820 [Arabidopsis thaliana]
gi|332193934|gb|AEE32055.1| aminoacylase [Arabidopsis
thaliana]
Length = 438
Score = 597 bits (1539), Expect = e-168
Identities = 290/416 (69%), Positives = 347/416 (83%)
Frame = -2
Query: 1305 EDTPITRFQQYLRIKTAHPTPDYTPAIKFLTHQAQSIPNLQIRTLDFSSSPNYTKPLLLL 1126
EDTPITRFQQYLR TAHP P+YT I FL +QAQSI L +T++F S KP+LL+
Sbjct: 26 EDTPITRFQQYLRFNTAHPNPNYTAPISFLINQAQSI-GLTTKTIEFISG----KPILLI 80
Query: 1125 TLPGSNPTLSSILFNSHIDSVPAEPEKWTHHPFAATLTAEGKIFARGAQDDKCIGMQYLE 946
T GSNP L SILFNSH+DSVPAE EKWT+ PF+A T +G I+ARGAQDDKCIG+QYLE
Sbjct: 81 TWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSAHKTIDGHIYARGAQDDKCIGVQYLE 140
Query: 945 TLRNIQKDSNFKMPVRTIHVSFVPDEEIGGFDGMMKFVESQEFKELNVGFVMDEGQTSTN 766
++RN+ K F P+RTIH+S+VP+EEIGGFDGMMKF S EFK+LN+GF MDEGQ +
Sbjct: 141 SIRNL-KSRGFS-PLRTIHISYVPEEEIGGFDGMMKFAASSEFKDLNLGFAMDEGQANPG 198
Query: 765 DEFRVFYADRAPWNLVIKAHGTPGHGSKLFDNSAMENLMRSVELITKFRENQFDLVKAGL 586
DEFRVFYADR PW+ VIKA G PGHG+KL+DNSAMENLM+SVELI++FRE+QFD VKAG
Sbjct: 199 DEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAMENLMKSVELISRFRESQFDFVKAGK 258
Query: 585 KLTSEVISANCVYLKAGTPSPTGFVMNMQPSEAEAGFDVRLPPTADSDMLRRRIAEEWAP 406
SEVIS N VYLKAGTP+ TGFVMNMQPSEAEAG+D+RLPP AD D++++RIAEEWAP
Sbjct: 259 AAYSEVISVNPVYLKAGTPTTTGFVMNMQPSEAEAGYDLRLPPMADPDVMKKRIAEEWAP 318
Query: 405 AIRNMTYKITQKGPLKDYAGRPLMTATNNSNPWWSVFKQTVIAAGGKLAKPEILASTTDA 226
+IRNMTY I +KG L+D+ GRP+MT N+SNPWWS+FKQ V A GGKLAKPEILASTTD+
Sbjct: 319 SIRNMTYSIQEKGKLRDHLGRPIMTPVNDSNPWWSIFKQAVEAMGGKLAKPEILASTTDS 378
Query: 225 RFMRQIGIPTLGFSPMKNTPILLHDHNEYLEASVYLEGIKVYESIFKALSSFEGDT 58
RF+R +GIPT GFSPM NTPILLHDHNE+L+ +V+++GI+VYES+ ALSSFEG++
Sbjct: 379 RFIRTLGIPTFGFSPMTNTPILLHDHNEFLKDTVFMKGIEVYESVISALSSFEGES 434
>dbj|BAE99837.1| aminoacylase like protein [Arabidopsis thaliana]
Length = 424
Score = 597 bits (1539), Expect = e-168
Identities = 290/416 (69%), Positives = 347/416 (83%)
Frame = -2
Query: 1305 EDTPITRFQQYLRIKTAHPTPDYTPAIKFLTHQAQSIPNLQIRTLDFSSSPNYTKPLLLL 1126
EDTPITRFQQYLR TAHP P+YT I FL +QAQSI L +T++F S KP+LL+
Sbjct: 12 EDTPITRFQQYLRFNTAHPNPNYTAPISFLINQAQSI-GLTTKTIEFISG----KPILLI 66
Query: 1125 TLPGSNPTLSSILFNSHIDSVPAEPEKWTHHPFAATLTAEGKIFARGAQDDKCIGMQYLE 946
T GSNP L SILFNSH+DSVPAE EKWT+ PF+A T +G I+ARGAQDDKCIG+QYLE
Sbjct: 67 TWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSAHKTIDGHIYARGAQDDKCIGVQYLE 126
Query: 945 TLRNIQKDSNFKMPVRTIHVSFVPDEEIGGFDGMMKFVESQEFKELNVGFVMDEGQTSTN 766
++RN+ K F P+RTIH+S+VP+EEIGGFDGMMKF S EFK+LN+GF MDEGQ +
Sbjct: 127 SIRNL-KSRGFS-PLRTIHISYVPEEEIGGFDGMMKFAASSEFKDLNLGFAMDEGQANPG 184
Query: 765 DEFRVFYADRAPWNLVIKAHGTPGHGSKLFDNSAMENLMRSVELITKFRENQFDLVKAGL 586
DEFRVFYADR PW+ VIKA G PGHG+KL+DNSAMENLM+SVELI++FRE+QFD VKAG
Sbjct: 185 DEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAMENLMKSVELISRFRESQFDFVKAGK 244
Query: 585 KLTSEVISANCVYLKAGTPSPTGFVMNMQPSEAEAGFDVRLPPTADSDMLRRRIAEEWAP 406
SEVIS N VYLKAGTP+ TGFVMNMQPSEAEAG+D+RLPP AD D++++RIAEEWAP
Sbjct: 245 AAYSEVISVNPVYLKAGTPTTTGFVMNMQPSEAEAGYDLRLPPMADPDVMKKRIAEEWAP 304
Query: 405 AIRNMTYKITQKGPLKDYAGRPLMTATNNSNPWWSVFKQTVIAAGGKLAKPEILASTTDA 226
+IRNMTY I +KG L+D+ GRP+MT N+SNPWWS+FKQ V A GGKLAKPEILASTTD+
Sbjct: 305 SIRNMTYSIQEKGKLRDHLGRPIMTPVNDSNPWWSIFKQAVEAMGGKLAKPEILASTTDS 364
Query: 225 RFMRQIGIPTLGFSPMKNTPILLHDHNEYLEASVYLEGIKVYESIFKALSSFEGDT 58
RF+R +GIPT GFSPM NTPILLHDHNE+L+ +V+++GI+VYES+ ALSSFEG++
Sbjct: 365 RFIRTLGIPTFGFSPMTNTPILLHDHNEFLKDTVFMKGIEVYESVISALSSFEGES 420
>ref|XP_003634462.1| PREDICTED: aminoacylase-1 isoform 3 [Vitis vinifera]
Length = 445
Score = 597 bits (1538), Expect = e-168
Identities = 294/420 (70%), Positives = 347/420 (82%)
Frame = -2
Query: 1329 EYNDNQECEDTPITRFQQYLRIKTAHPTPDYTPAIKFLTHQAQSIPNLQIRTLDFSSSPN 1150
+++ +E ED+PITRFQ YLRI T HP PDY A+ FL QA I LQ +TL+F+ S
Sbjct: 33 QHHLEKEEEDSPITRFQGYLRINTVHPNPDYASAVSFLKSQALYI-GLQTKTLEFARS-- 89
Query: 1149 YTKPLLLLTLPGSNPTLSSILFNSHIDSVPAEPEKWTHHPFAATLTAEGKIFARGAQDDK 970
KPLLLLT PGS+P+L SIL NSH+DSVP EP KW H PF+A + +GKIFARG+QDDK
Sbjct: 90 --KPLLLLTWPGSDPSLPSILLNSHLDSVPVEPSKWLHPPFSAYRSPDGKIFARGSQDDK 147
Query: 969 CIGMQYLETLRNIQKDSNFKMPVRTIHVSFVPDEEIGGFDGMMKFVESQEFKELNVGFVM 790
CI MQYLE +RN++ NF+ P RTIH+S+VPDEEIGGFDG KFV S+EF +LNVGF++
Sbjct: 148 CIAMQYLEAIRNLRAQ-NFQ-PTRTIHISYVPDEEIGGFDGAAKFVASKEFADLNVGFML 205
Query: 789 DEGQTSTNDEFRVFYADRAPWNLVIKAHGTPGHGSKLFDNSAMENLMRSVELITKFRENQ 610
DEGQ ST DEFRVFYADR+PWNL+IKA G PGHGS+L+DNSAMENLM+SVE+ITKFRE+
Sbjct: 206 DEGQASTGDEFRVFYADRSPWNLIIKAFGMPGHGSRLYDNSAMENLMKSVEIITKFRESL 265
Query: 609 FDLVKAGLKLTSEVISANCVYLKAGTPSPTGFVMNMQPSEAEAGFDVRLPPTADSDMLRR 430
FD+VKAG SEVIS N VYLKAG PSPTGFVMNMQPSEAEAGFD+R+PPTAD D+++
Sbjct: 266 FDVVKAGKAANSEVISVNPVYLKAGIPSPTGFVMNMQPSEAEAGFDLRMPPTADPDLVKI 325
Query: 429 RIAEEWAPAIRNMTYKITQKGPLKDYAGRPLMTATNNSNPWWSVFKQTVIAAGGKLAKPE 250
RIAEEWAPAIRNMTY++ + DY GRPLMT TN+SNPWWS+FKQ + AGGKLAKPE
Sbjct: 326 RIAEEWAPAIRNMTYQLKE-----DYMGRPLMTLTNDSNPWWSIFKQAITEAGGKLAKPE 380
Query: 249 ILASTTDARFMRQIGIPTLGFSPMKNTPILLHDHNEYLEASVYLEGIKVYESIFKALSSF 70
ILASTTDAR+MRQ+GIPTLGFSPM NTPILLHDHNE+L+ ++YL GIKVYES+ +LSSF
Sbjct: 381 ILASTTDARYMRQMGIPTLGFSPMTNTPILLHDHNEFLKDTIYLRGIKVYESVISSLSSF 440