BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004637
         (1620 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAT57675.1| proline oxidase/dehydrogenase 2 [Nicotiana tabacum]    580   e-163
dbj|BAB83948.1| CIG1 [Nicotiana tabacum]                              577   e-162
gb|AAT57674.1| proline oxidase/dehydrogenase 1 [Nicotiana tabacum]    575   e-161
gb|ABK91948.1| proline dehydrogenase [Actinidia deliciosa]            566   e-159
gb|ACR08418.1| proline oxidase/dehydrogenase 1 [Capsicum annuum]      566   e-159

>gb|AAT57675.1| proline oxidase/dehydrogenase 2 [Nicotiana tabacum]
          Length = 499

 Score =  580 bits (1494), Expect = e-163
 Identities = 282/430 (65%), Positives = 350/430 (81%)
 Frame = +1

Query: 208  FEDNKDLFSSVPTGKLLKSALTLKMAAVEPVVDLGTWVVNSRLMNVGVFKEMVIGLTKLT 387
            F+D K+LF  VPT KL++S+LTL+MAA+EP+VD+G WV+NS+LM + +F+E+++G  K T
Sbjct: 69   FDDVKELFYGVPTSKLIRSSLTLQMAAIEPMVDMGMWVMNSKLMEMPIFREVMLGFVKNT 128

Query: 388  FYDHFVAGRCVEEAAETVKTLWSDGFRGMLDYGLEHVDDNCSCDRNMEVLIQTVDSTKSL 567
            FY+HF AG+ + E   TV  L   G + MLDYG+EH   N SC+++M+  IQT++STKSL
Sbjct: 129  FYEHFCAGKDLTEVRRTVSKLSDGGLKAMLDYGVEHATHNESCEQSMKAFIQTIESTKSL 188

Query: 568  PATSVSSVVVKITAICPVSLLKRVSHLLRWEYKTNSINLPWKQNTFPLFSESSPLYHTQQ 747
            P +S S VVVKITAIC   LLKR+S LLRWE+K  S NLPWKQ T PLF+ESSPLYHT +
Sbjct: 189  PQSSTSFVVVKITAICTPRLLKRMSDLLRWEHKDPSFNLPWKQKTLPLFAESSPLYHTSK 248

Query: 748  KPDPLSLQEEQDLQLAHQRLTNIIQKCLESNIPVVIDAEETLIQPAIDYFTYSAAILYNK 927
            KP+PL+++EE+DLQLAH+RLT I +KCLE ++P++IDAE+T IQP IDY  YSAAI Y+K
Sbjct: 249  KPEPLTVEEERDLQLAHKRLTKICEKCLEHDVPLLIDAEDTTIQPGIDYMAYSAAIKYHK 308

Query: 928  GDKPLIFGTFQAYLKDAKERLMLAKSAADKMGLPLGVKLVRGAYMSTEGQVAASLGHESP 1107
             D PLIFGT QAYLKDAKER+ +AK AA+KMG+P+G KLVRGAYM +E ++A+ LG +SP
Sbjct: 309  DDGPLIFGTIQAYLKDAKERMAIAKKAAEKMGVPMGFKLVRGAYMCSERELASRLGFQSP 368

Query: 1108 VHNGIKQTHACYNDCASFMLNEIANGPGALVLATHNSESGKLAALKAQELGIGKGDQKVQ 1287
            +H+ I+QTHAC+N CA FM+ EIANG GA+VLATHN ESGKLAA KA +LGI K  QK+Q
Sbjct: 369  IHDSIEQTHACFNSCAEFMIEEIANGSGAVVLATHNIESGKLAATKAIDLGI-KDSQKLQ 427

Query: 1288 FAQLYGMAEALSYGLRNAGFAVSKYLPFGPVDQIMPYLLRRAEENKGMLSSSTIDRQLMR 1467
            FAQLYGMAE LS+GLRNAGF VSKYLPFGPVDQIM YL+RRAEEN+GMLS+S  DRQLMR
Sbjct: 428  FAQLYGMAEGLSFGLRNAGFQVSKYLPFGPVDQIMHYLMRRAEENRGMLSTSAFDRQLMR 487

Query: 1468 MELKRRLTAA 1497
             EL RRL  A
Sbjct: 488  KELSRRLKVA 497


>dbj|BAB83948.1| CIG1 [Nicotiana tabacum]
          Length = 493

 Score =  577 bits (1486), Expect = e-162
 Identities = 280/443 (63%), Positives = 357/443 (80%)
 Frame = +1

Query: 169  SDKPTTTAASPLCFEDNKDLFSSVPTGKLLKSALTLKMAAVEPVVDLGTWVVNSRLMNVG 348
            +D+  T     + FED K+LF+ V T KL++S LTL+MAA EP+VD+G WV+NS+LM++ 
Sbjct: 49   TDQVITADKKVINFEDVKELFTGVSTLKLIRSTLTLQMAATEPMVDVGIWVMNSKLMHMP 108

Query: 349  VFKEMVIGLTKLTFYDHFVAGRCVEEAAETVKTLWSDGFRGMLDYGLEHVDDNCSCDRNM 528
            + KE+++G  K TFY+HF AG+ + E   TV  L   G +GMLDYG+EH  +N SCD++M
Sbjct: 109  IVKEVILGFVKGTFYEHFCAGKDLIEVRRTVTKLSDVGLKGMLDYGVEHATENESCDQSM 168

Query: 529  EVLIQTVDSTKSLPATSVSSVVVKITAICPVSLLKRVSHLLRWEYKTNSINLPWKQNTFP 708
            +V +QT +STKSLP++SVS VVVKITAIC   LLKR+S LLRWE+K  S NLPWKQ + P
Sbjct: 169  KVFLQTAESTKSLPSSSVSFVVVKITAICTPKLLKRMSDLLRWEHKNPSFNLPWKQKSLP 228

Query: 709  LFSESSPLYHTQQKPDPLSLQEEQDLQLAHQRLTNIIQKCLESNIPVVIDAEETLIQPAI 888
            LFS+SSP YHT QKP+PL+++EE DLQLAH+RL  I +KCLE ++ ++IDAE+T IQPAI
Sbjct: 229  LFSDSSPFYHTPQKPEPLTVEEEHDLQLAHERLMTICKKCLELDVDLLIDAEDTAIQPAI 288

Query: 889  DYFTYSAAILYNKGDKPLIFGTFQAYLKDAKERLMLAKSAADKMGLPLGVKLVRGAYMST 1068
            DYF YSAAI Y+K D P+IFGT QAYLKD+KER+++AK AA+KMG+P+G KLVRGAYMS+
Sbjct: 289  DYFAYSAAIKYHKDDDPMIFGTIQAYLKDSKERMVIAKKAAEKMGVPMGFKLVRGAYMSS 348

Query: 1069 EGQVAASLGHESPVHNGIKQTHACYNDCASFMLNEIANGPGALVLATHNSESGKLAALKA 1248
            E ++A+ LG +SP+H+ I+QTH C+N CA FML+EI+NG GA+VLATHN +SGKLAA KA
Sbjct: 349  ERELASRLGVQSPIHDSIEQTHDCFNSCAEFMLDEISNGSGAVVLATHNIDSGKLAASKA 408

Query: 1249 QELGIGKGDQKVQFAQLYGMAEALSYGLRNAGFAVSKYLPFGPVDQIMPYLLRRAEENKG 1428
             +LGI K  QK+QFAQLYGMAE LS+GLRNAGF VSKYLPFGPV+Q+MPYL+RRAEEN+G
Sbjct: 409  IDLGIRKDSQKLQFAQLYGMAEGLSFGLRNAGFQVSKYLPFGPVEQVMPYLIRRAEENRG 468

Query: 1429 MLSSSTIDRQLMRMELKRRLTAA 1497
            +LS+S  DRQLMR EL RR   A
Sbjct: 469  LLSTSAFDRQLMRKELTRRFKVA 491


>gb|AAT57674.1| proline oxidase/dehydrogenase 1 [Nicotiana tabacum]
          Length = 499

 Score =  575 bits (1481), Expect = e-161
 Identities = 278/430 (64%), Positives = 349/430 (81%)
 Frame = +1

Query: 208  FEDNKDLFSSVPTGKLLKSALTLKMAAVEPVVDLGTWVVNSRLMNVGVFKEMVIGLTKLT 387
            F+D K+LF  VPT KL++S LTL+MAA++P+VD+G WV+NS+LM + +F+E+++G  K T
Sbjct: 69   FDDVKELFYGVPTSKLIRSTLTLQMAAIDPMVDMGMWVMNSKLMEMPIFREVMLGFVKNT 128

Query: 388  FYDHFVAGRCVEEAAETVKTLWSDGFRGMLDYGLEHVDDNCSCDRNMEVLIQTVDSTKSL 567
            FY+HF AG+ + E   TV  L   G + MLDYG+EH  +N SC+++M+  IQT++STKSL
Sbjct: 129  FYEHFCAGKDLTEVRRTVSKLSDGGLKAMLDYGVEHATNNESCEQSMKAFIQTIESTKSL 188

Query: 568  PATSVSSVVVKITAICPVSLLKRVSHLLRWEYKTNSINLPWKQNTFPLFSESSPLYHTQQ 747
            P +S S VVVKITAIC   LLKR+S LLRWE+K  S NLPWKQ T PLF+ESSPLYHT +
Sbjct: 189  PQSSTSFVVVKITAICTPRLLKRMSDLLRWEHKDPSFNLPWKQKTLPLFAESSPLYHTSK 248

Query: 748  KPDPLSLQEEQDLQLAHQRLTNIIQKCLESNIPVVIDAEETLIQPAIDYFTYSAAILYNK 927
            KP+PL+++EE+DLQLA +RLT I +KCLE ++P++IDAE+T IQP IDY  YSAAI Y+K
Sbjct: 249  KPEPLTVEEERDLQLARERLTKICEKCLEHDVPLLIDAEDTTIQPGIDYMAYSAAIKYHK 308

Query: 928  GDKPLIFGTFQAYLKDAKERLMLAKSAADKMGLPLGVKLVRGAYMSTEGQVAASLGHESP 1107
             D PLIFGT QAYLKDAKER+ +AK AA+KMG+P+G KLVRGAYM +E ++A+ LG +SP
Sbjct: 309  DDDPLIFGTIQAYLKDAKERMAIAKKAAEKMGVPMGFKLVRGAYMCSERELASRLGFQSP 368

Query: 1108 VHNGIKQTHACYNDCASFMLNEIANGPGALVLATHNSESGKLAALKAQELGIGKGDQKVQ 1287
            +H+ I+QTHAC+N CA FM+ EIANG GA+VLATHN ESGKLAA KA ++GI K  QK+Q
Sbjct: 369  IHDSIEQTHACFNSCAEFMIEEIANGSGAVVLATHNIESGKLAATKAIDMGI-KDSQKLQ 427

Query: 1288 FAQLYGMAEALSYGLRNAGFAVSKYLPFGPVDQIMPYLLRRAEENKGMLSSSTIDRQLMR 1467
            FAQLYGMAE L++GLRNAGF VSKYLPFGPVDQIM YL+RRAEEN+GMLS+S  DRQLMR
Sbjct: 428  FAQLYGMAEGLTFGLRNAGFQVSKYLPFGPVDQIMHYLMRRAEENRGMLSTSAFDRQLMR 487

Query: 1468 MELKRRLTAA 1497
             EL RRL  A
Sbjct: 488  KELSRRLKVA 497


>gb|ABK91948.1| proline dehydrogenase [Actinidia deliciosa]
          Length = 508

 Score =  567 bits (1460), Expect = e-159
 Identities = 281/442 (63%), Positives = 346/442 (78%), Gaps = 1/442 (0%)
 Frame = +1

Query: 178  PTTTAASPLCFEDNKDLFSSVPTGKLLKSALTLKMAAVEPVVDLGTWVVNSRLMNVGVFK 357
            PTTT    L     K+LFSSVPT KLL+SA  L +AA+EP+VDLG WV+NS+LM   V +
Sbjct: 69   PTTTI---LNLNAVKELFSSVPTLKLLRSAANLHVAAIEPMVDLGIWVMNSKLMTAPVAR 125

Query: 358  EMVIGLTKLTFYDHFVAGRCVEEAAETVKTLWSDGFRGMLDYGLEHVDDNCSCDRNMEVL 537
            E+ +G  K TFY+HF AGR   EAA T   LW  G +GML Y LEH  DN SCD N++  
Sbjct: 126  EVTVGAIKHTFYEHFCAGRGPVEAAGTAAKLWDSGLKGMLVYALEHAADNESCDWNLDAF 185

Query: 538  IQTVDSTKSLPATSVSSVVVKITAICPVSLLKRVSHLLRWEYKTN-SINLPWKQNTFPLF 714
            ++TV+S KSL   SVSS VVKITAICP+ LL+RVS LLRWEY  N S  LPWK NT P+F
Sbjct: 186  LKTVESIKSLATCSVSSTVVKITAICPLRLLRRVSDLLRWEYNNNQSFQLPWKLNTLPVF 245

Query: 715  SESSPLYHTQQKPDPLSLQEEQDLQLAHQRLTNIIQKCLESNIPVVIDAEETLIQPAIDY 894
            S+SSP YHT  KPDPL+ +EE DL LA QR+  + QKC+E  +P+++DAE+T +QP IDY
Sbjct: 246  SDSSPFYHTPIKPDPLTPEEESDLHLAQQRMLKLCQKCVEVGVPLLVDAEDTSVQPGIDY 305

Query: 895  FTYSAAILYNKGDKPLIFGTFQAYLKDAKERLMLAKSAADKMGLPLGVKLVRGAYMSTEG 1074
            FTYSAA+++NKGD P+I+GT Q YLKDA+ERL LA   A++MG+ +G KLVRGAYMS+E 
Sbjct: 306  FTYSAALMHNKGDNPIIYGTIQTYLKDARERLFLAAREAERMGVSIGFKLVRGAYMSSER 365

Query: 1075 QVAASLGHESPVHNGIKQTHACYNDCASFMLNEIANGPGALVLATHNSESGKLAALKAQE 1254
            ++AASLG ESP+HN I QTHAC+NDCASFML +IANG GA+VLATHN ESG  AA+KA +
Sbjct: 366  ELAASLGVESPIHNTIHQTHACFNDCASFMLEKIANGSGAVVLATHNIESGNKAAVKATD 425

Query: 1255 LGIGKGDQKVQFAQLYGMAEALSYGLRNAGFAVSKYLPFGPVDQIMPYLLRRAEENKGML 1434
            LGI KG++ +QFAQLYGMA+ALS+GLRNAGF VSKY+PFGPV++IMPYLLRRAEEN+G+L
Sbjct: 426  LGIKKGNENLQFAQLYGMADALSFGLRNAGFQVSKYMPFGPVERIMPYLLRRAEENRGLL 485

Query: 1435 SSSTIDRQLMRMELKRRLTAAV 1500
             +ST+DRQL+R EL RRL  A+
Sbjct: 486  CASTLDRQLLRKELMRRLKVAI 507


>gb|ACR08418.1| proline oxidase/dehydrogenase 1 [Capsicum annuum]
          Length = 501

 Score =  566 bits (1458), Expect = e-159
 Identities = 274/430 (63%), Positives = 347/430 (80%)
 Frame = +1

Query: 208  FEDNKDLFSSVPTGKLLKSALTLKMAAVEPVVDLGTWVVNSRLMNVGVFKEMVIGLTKLT 387
            F D K+LF  VPT KL++S+LTL+MAA+EP+VDLG WV+NS+LM + VF+++++G  K T
Sbjct: 70   FNDVKELFYGVPTTKLIRSSLTLQMAAIEPMVDLGMWVMNSKLMEMPVFRDVMLGFVKNT 129

Query: 388  FYDHFVAGRCVEEAAETVKTLWSDGFRGMLDYGLEHVDDNCSCDRNMEVLIQTVDSTKSL 567
            FY+HF AG+ + E   TV  L   G + MLDYG+EH  +N SC+++    IQT++STKSL
Sbjct: 130  FYEHFCAGKDLTEVRRTVMNLSDSGLKAMLDYGVEHATENESCEQSTTAFIQTIESTKSL 189

Query: 568  PATSVSSVVVKITAICPVSLLKRVSHLLRWEYKTNSINLPWKQNTFPLFSESSPLYHTQQ 747
            P +S S VV KITAIC   LLKR+S LLRWE K  S+NLPWK+ T PLF+ESSP+YHT +
Sbjct: 190  PQSSASFVVAKITAICTPRLLKRMSDLLRWEQKDPSLNLPWKRRTLPLFAESSPVYHTSE 249

Query: 748  KPDPLSLQEEQDLQLAHQRLTNIIQKCLESNIPVVIDAEETLIQPAIDYFTYSAAILYNK 927
            KP+PL+++EE+DL+LAH+RL  I +KCLE ++P++IDAE++ IQPAIDYF YSAAI Y+K
Sbjct: 250  KPEPLTVEEERDLELAHERLKKICEKCLEHDVPLLIDAEDSTIQPAIDYFAYSAAIKYHK 309

Query: 928  GDKPLIFGTFQAYLKDAKERLMLAKSAADKMGLPLGVKLVRGAYMSTEGQVAASLGHESP 1107
             D+PLIFGT QAYLKDAKER+++AK AA+KMG+P+G KLVRGAYM +E  +A+SLG  SP
Sbjct: 310  DDQPLIFGTIQAYLKDAKERMVMAKKAAEKMGVPMGFKLVRGAYMCSEKALASSLGINSP 369

Query: 1108 VHNGIKQTHACYNDCASFMLNEIANGPGALVLATHNSESGKLAALKAQELGIGKGDQKVQ 1287
            +H+ I+QTHAC+N CA FM+ EIANG GA+VLATH+ ESGKLAA KA +LGI    Q +Q
Sbjct: 370  IHDSIEQTHACFNSCAEFMIEEIANGSGAVVLATHDIESGKLAATKAIDLGIKIERQSLQ 429

Query: 1288 FAQLYGMAEALSYGLRNAGFAVSKYLPFGPVDQIMPYLLRRAEENKGMLSSSTIDRQLMR 1467
            FAQLYGMA+ LS+GLRNAGF VSKYLPFGPV+QIM YL+RRAEEN+GMLS+S  DRQLMR
Sbjct: 430  FAQLYGMADGLSFGLRNAGFQVSKYLPFGPVEQIMHYLMRRAEENRGMLSTSAFDRQLMR 489

Query: 1468 MELKRRLTAA 1497
             EL RRL  A
Sbjct: 490  KELSRRLEVA 499