BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004457
         (1568 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002270055.1| PREDICTED: bifunctional 3-dehydroquinate deh...   682   0.0  
gb|AAS90324.2| 3-dehydroquinate dehydratase / shikimate dehydrog...   675   0.0  
ref|XP_002512298.1| shikimate dehydrogenase, putative [Ricinus c...   671   0.0  
ref|XP_002319583.1| dehydroquinate dehydratase/ shikimate dehydr...   661   0.0  
ref|XP_002328402.1| dehydroquinate dehydratase/ shikimate dehydr...   656   0.0  

>ref|XP_002270055.1| PREDICTED: bifunctional 3-dehydroquinate dehydratase/shikimate
            dehydrogenase, chloroplastic [Vitis vinifera]
            gi|298204989|emb|CBI34296.3| unnamed protein product
            [Vitis vinifera]
          Length = 519

 Score =  682 bits (1761), Expect = 0.0
 Identities = 337/509 (66%), Positives = 413/509 (81%), Gaps = 1/509 (0%)
 Frame = +3

Query: 9    TTVCALLMSQSVEQMVSDMYKAKAQGADIVELRLDCLKSFSFPQDLQLLLTNKPLPVIIV 188
            T +C  LM QSVEQMV DM+KAK +GAD+VE+RLD + +F   QDL+++L NKPLPV+IV
Sbjct: 11   TMICTPLMGQSVEQMVRDMHKAKVEGADLVEVRLDYINNFHPQQDLEIILRNKPLPVMIV 70

Query: 189  CRPTSEGGEYEGDENTRIEVLRTAKDLGADYIDIELKVAFSLMEEQKFSQHGGSKIIVSC 368
             RP  EGG+YEGDE++R+E L  A+ LGADYID ELKVA   + +QK  QH  S+ IVSC
Sbjct: 71   YRPKWEGGQYEGDEHSRLEALHLAEKLGADYIDFELKVASDFLGKQKMDQHSSSRTIVSC 130

Query: 369  FLDTVTYAKEDLSQVVAHIQSTGADMIKLVSYTSSITDLSKTFHLLSHSQVPLIAYTTGE 548
            ++D VT   EDL   VA +QSTGADMIKLV   ++IT+++K FHLLSH Q+PLIAY+ G+
Sbjct: 131  YVDGVTPPIEDLICRVALLQSTGADMIKLVINATNITEITKIFHLLSHCQMPLIAYSIGD 190

Query: 549  RGLITQLVGPKFGGHFVYGSVDGNQVLGLPTLYSLRQAYGVEHVDSDTKIFGLISKPVSH 728
            RG ++Q++  KFGG  VYGS++G+ V GLPTL SLR+AY V++++ DTK+FGLISKPV H
Sbjct: 191  RGFMSQILCRKFGGFLVYGSMEGSPVAGLPTLESLREAYKVQYINKDTKVFGLISKPVGH 250

Query: 729  SKGPILHNPTFRYVGYNGIYVPMFVDDLKDFFRVYSS-DFAGYSVGIPYKEAIIEFCDEV 905
            SKGPILHNP FR+V YNGIYVPM VDDLK+FF +YSS DFAG+SVGIPYKEA+  FCDE+
Sbjct: 251  SKGPILHNPAFRHVNYNGIYVPMLVDDLKEFFSIYSSPDFAGFSVGIPYKEAVTGFCDEL 310

Query: 906  HPLAQSIGAVNTIVRRSSDGKLVGYNTDCEASITAIEDTIKVLGCVNGEALLPSPITGKQ 1085
            HPLAQSIGAVNTI+RR SDGKL+GYNTDCEASITAIED ++  G  NGEA L SP+TGKQ
Sbjct: 311  HPLAQSIGAVNTIMRRPSDGKLIGYNTDCEASITAIEDALRERGLPNGEAPLNSPLTGKQ 370

Query: 1086 XXXXXXXXXXXXXXXXXKTRGARVIVFDISFDRAKSLADAVCGEVKPYDDLPNFQPEKGA 1265
                             ++RGA++++FD+ FDRA SLA AV GEVK Y+D+ NFQPEKGA
Sbjct: 371  FVLVGAGGAGRALAFGARSRGAQLVIFDLDFDRANSLAHAVSGEVKLYEDVANFQPEKGA 430

Query: 1266 ILANATPIGMHPNTDRIPVAKETLKDYKLVFDAVYTPRKTTLLRDAESAGALIVSGVEMF 1445
            ILANATP+GMHPNTDRIPVA+ETL DY+LVFD+VYTPRKT LL++AE+AGA+IVSGVEMF
Sbjct: 431  ILANATPVGMHPNTDRIPVAEETLSDYQLVFDSVYTPRKTRLLKEAEAAGAIIVSGVEMF 490

Query: 1446 LRQAMGQFNLFTGGQAPEKFMRAIVMEKF 1532
            LRQA+GQFNLFTGG+APE+FMR I++ KF
Sbjct: 491  LRQAIGQFNLFTGGEAPEEFMREIILSKF 519


>gb|AAS90324.2| 3-dehydroquinate dehydratase / shikimate dehydrogenase isoform 2
            [Nicotiana tabacum]
          Length = 521

 Score =  675 bits (1741), Expect = 0.0
 Identities = 342/514 (66%), Positives = 415/514 (80%), Gaps = 4/514 (0%)
 Frame = +3

Query: 3    NTTTVCALLMSQSVEQMVSDMYKAKAQGADIVELRLDCLKSFSFPQDLQLLLTNKPLPVI 182
            N+  VCA LM+ SVEQ+V  M +AKAQGAD+VE+RLD + +F   +DLQ+LL N PLPV+
Sbjct: 9    NSAMVCAPLMAPSVEQLVHGMLQAKAQGADLVEIRLDGINNFQPQKDLQVLLNNNPLPVL 68

Query: 183  IVCRPTSEGGEYEGDEN---TRIEVLRTAKDLGADYIDIELKVAFSLMEEQKFSQHGGSK 353
            IV RP  EG E+E D++    ++EVLR AK+LGADYI+++LK+A    +++K     G K
Sbjct: 69   IVYRPIWEGNEFEEDDDHIHKQLEVLRWAKELGADYIELDLKIASDFTKKEKPRWSSGCK 128

Query: 354  IIVSCFLDTVTYAKEDLSQVVAHIQSTGADMIKLVSYTSSITDLSKTFHLLSHSQVPLIA 533
            +I SCF+D VT +KEDLSQVVAH+QSTGAD++K+V+  + IT+L K FHLLSH QVPLIA
Sbjct: 129  VIASCFVDNVT-SKEDLSQVVAHMQSTGADILKIVTNANDITELEKMFHLLSHCQVPLIA 187

Query: 534  YTTGERGLITQLVGPKFGGHFVYGSVDGNQVLGLPTLYSLRQAYGVEHVDSDTKIFGLIS 713
            Y+ GERGLI+QL+GPKFG   VYGS+D N V GLPTL SLRQAYGV+ +D+DTK+FGLIS
Sbjct: 188  YSLGERGLISQLLGPKFGSVLVYGSLDCNAVPGLPTLGSLRQAYGVDFMDTDTKVFGLIS 247

Query: 714  KPVSHSKGPILHNPTFRYVGYNGIYVPMFVDDLKDFFRVYSS-DFAGYSVGIPYKEAIIE 890
            KPV HSKGPILHNPTFR+VGYNGIYVPMFVDDLK+FFRVYSS DFAG+SVGIPYKEA++ 
Sbjct: 248  KPVGHSKGPILHNPTFRHVGYNGIYVPMFVDDLKEFFRVYSSPDFAGFSVGIPYKEAVVS 307

Query: 891  FCDEVHPLAQSIGAVNTIVRRSSDGKLVGYNTDCEASITAIEDTIKVLGCVNGEALLPSP 1070
            FCDEV PLA+SIGAVNTI++R  DGKL+GYNTDCEASITAIED +KV G  NG A LPSP
Sbjct: 308  FCDEVDPLAKSIGAVNTIIQRPCDGKLIGYNTDCEASITAIEDALKVNGLTNGAAFLPSP 367

Query: 1071 ITGKQXXXXXXXXXXXXXXXXXKTRGARVIVFDISFDRAKSLADAVCGEVKPYDDLPNFQ 1250
            + GK                  K+R A +++FDI FDRAK+LA AV GE  P+++L +FQ
Sbjct: 368  LAGKLFVLVGAGGAGRALAFGAKSRRAEIVIFDIDFDRAKALAAAVSGEALPFENLASFQ 427

Query: 1251 PEKGAILANATPIGMHPNTDRIPVAKETLKDYKLVFDAVYTPRKTTLLRDAESAGALIVS 1430
            PEKGAILANATPIGMHPN DRIPV++ +LKDY +VFDAVYTPRKTTLL+DAE+AGA+ VS
Sbjct: 428  PEKGAILANATPIGMHPNKDRIPVSEASLKDYVVVFDAVYTPRKTTLLKDAEAAGAITVS 487

Query: 1431 GVEMFLRQAMGQFNLFTGGQAPEKFMRAIVMEKF 1532
            GVEMFLRQA+GQF+LFT  +APE+FMR IVM KF
Sbjct: 488  GVEMFLRQAIGQFHLFTRTKAPEEFMRDIVMAKF 521


>ref|XP_002512298.1| shikimate dehydrogenase, putative [Ricinus communis]
            gi|223548259|gb|EEF49750.1| shikimate dehydrogenase,
            putative [Ricinus communis]
          Length = 519

 Score =  671 bits (1732), Expect = 0.0
 Identities = 332/511 (64%), Positives = 412/511 (80%), Gaps = 1/511 (0%)
 Frame = +3

Query: 3    NTTTVCALLMSQSVEQMVSDMYKAKAQGADIVELRLDCLKSFSFPQDLQLLLTNKPLPVI 182
            ++  VC  LM+QSVEQM+SDMY AK QGAD+VE+RLD + +F  PQDLQ +L NKPLPVI
Sbjct: 9    SSVMVCTPLMAQSVEQMISDMYNAKTQGADVVEVRLDYIDNFQPPQDLQAILRNKPLPVI 68

Query: 183  IVCRPTSEGGEYEGDENTRIEVLRTAKDLGADYIDIELKVAFSLMEEQKFSQHGGSKIIV 362
            IV RP SEGG YEGDE TR+E LR A  LGADY+D ELKVA  L+ E K + H GSK+IV
Sbjct: 69   IVYRPKSEGGLYEGDEPTRLEALRLAYVLGADYVDFELKVASDLIGELKGTHHTGSKVIV 128

Query: 363  SCFLDTVTYAKEDLSQVVAHIQSTGADMIKLVSYTSSITDLSKTFHLLSHSQVPLIAYTT 542
            SC+++     KE+LSQ+VA +Q+TGAD+IKLVS  ++IT+L + FHL+ H QVP+IAY+ 
Sbjct: 129  SCYVNGDMPTKENLSQLVASMQATGADIIKLVSTANNITELDRIFHLILHCQVPIIAYSV 188

Query: 543  GERGLITQLVGPKFGGHFVYGSVDGNQVLGLPTLYSLRQAYGVEHVDSDTKIFGLISKPV 722
            GERGLI+QL+ PKFGG  +YGS++G+ + GLPTL SLR+AY V +++SDTK+FGL+SKPV
Sbjct: 189  GERGLISQLLSPKFGGSLLYGSMEGSSIPGLPTLDSLREAYKVAYINSDTKVFGLVSKPV 248

Query: 723  SHSKGPILHNPTFRYVGYNGIYVPMFVDDLKDFFRVYSS-DFAGYSVGIPYKEAIIEFCD 899
            SHSKGP+LHNPTFR+  YNG YVPMFVDDLK+FF VYSS DFAG+SVG PYKEA++EFCD
Sbjct: 249  SHSKGPLLHNPTFRHANYNGTYVPMFVDDLKEFFSVYSSPDFAGFSVGFPYKEAVVEFCD 308

Query: 900  EVHPLAQSIGAVNTIVRRSSDGKLVGYNTDCEASITAIEDTIKVLGCVNGEALLPSPITG 1079
            EVHPLA+SIGAVNTI+RR  DGKL+G+NTDCEA+ITAIED +K  G ++G     SP+TG
Sbjct: 309  EVHPLAKSIGAVNTIIRRPGDGKLIGHNTDCEAAITAIEDALKEQGYMDGRTSSNSPLTG 368

Query: 1080 KQXXXXXXXXXXXXXXXXXKTRGARVIVFDISFDRAKSLADAVCGEVKPYDDLPNFQPEK 1259
            +Q                 K+RGAR+++FDI  +RAK LADAV GE + ++++ NF+PE 
Sbjct: 369  RQFVLVGAGGAGRALAFGAKSRGARIVIFDIDLERAKFLADAVSGEAQLFENVVNFEPEN 428

Query: 1260 GAILANATPIGMHPNTDRIPVAKETLKDYKLVFDAVYTPRKTTLLRDAESAGALIVSGVE 1439
            GAILANATPIGMHPNT+RIPVA+ TL  Y+LVFDAVYTPRKT LL++AE+AGA+IVSGVE
Sbjct: 429  GAILANATPIGMHPNTERIPVAEATLGIYQLVFDAVYTPRKTRLLKEAEAAGAIIVSGVE 488

Query: 1440 MFLRQAMGQFNLFTGGQAPEKFMRAIVMEKF 1532
            MFLRQAMGQF+LFTG +AP +FMR IV+ KF
Sbjct: 489  MFLRQAMGQFSLFTGREAPTEFMREIVLAKF 519


>ref|XP_002319583.1| dehydroquinate dehydratase/ shikimate dehydrogenase [Populus
            trichocarpa] gi|222857959|gb|EEE95506.1| dehydroquinate
            dehydratase/ shikimate dehydrogenase [Populus
            trichocarpa]
          Length = 518

 Score =  661 bits (1706), Expect = 0.0
 Identities = 333/511 (65%), Positives = 406/511 (79%), Gaps = 1/511 (0%)
 Frame = +3

Query: 3    NTTTVCALLMSQSVEQMVSDMYKAKAQGADIVELRLDCLKSFSFPQDLQLLLTNKPLPVI 182
            N+T VCA LM++SVEQMV DM  A+AQGAD VE+RLD + SF   QDL+ ++ NKPLPVI
Sbjct: 9    NSTMVCAPLMARSVEQMVIDMQSAEAQGADAVEVRLDYINSFQPSQDLETIIRNKPLPVI 68

Query: 183  IVCRPTSEGGEYEGDENTRIEVLRTAKDLGADYIDIELKVAFSLMEEQKFSQHGGSKIIV 362
            IV RP  EGG+YEGDE+TR+E LR A +LGADYID+ELKVA  L+ E K     G K+IV
Sbjct: 69   IVYRPRWEGGQYEGDEHTRLEALRLAHELGADYIDVELKVASDLVREVKNKHQTGGKVIV 128

Query: 363  SCFLDTVTYAKEDLSQVVAHIQSTGADMIKLVSYTSSITDLSKTFHLLSHSQVPLIAYTT 542
            S +L   T +KEDLS +VA +Q+T AD+IK+VS  + IT+L + FHLLSH QVP +AY+ 
Sbjct: 129  SSYLSGATPSKEDLSHLVASMQATKADIIKVVSNANDITELDRIFHLLSHMQVPAVAYSL 188

Query: 543  GERGLITQLVGPKFGGHFVYGSVDGNQVLGLPTLYSLRQAYGVEHVDSDTKIFGLISKPV 722
            GERGLI+QL+ PKFGG  VYG+++GN + GLPTL SLR+AY VE+++SDTK+FGL+SKPV
Sbjct: 189  GERGLISQLLCPKFGGALVYGAMEGNSIPGLPTLDSLREAYKVENINSDTKVFGLVSKPV 248

Query: 723  SHSKGPILHNPTFRYVGYNGIYVPMFVDDLKDFFRVYSS-DFAGYSVGIPYKEAIIEFCD 899
            SHSKGPILHNP FR+  +NGIYVPMFVDDLK+FF VY+S DFAGYSVG PYKEA+++FCD
Sbjct: 249  SHSKGPILHNPAFRHANFNGIYVPMFVDDLKEFFEVYASPDFAGYSVGFPYKEAVVQFCD 308

Query: 900  EVHPLAQSIGAVNTIVRRSSDGKLVGYNTDCEASITAIEDTIKVLGCVNGEALLPSPITG 1079
            EVHPLA+SIGAVNTI+R+ SDGKL+GYNTDCE SI +IED +K    +NG A L SP+ G
Sbjct: 309  EVHPLAKSIGAVNTIIRKPSDGKLIGYNTDCEGSIASIEDALKDQRYING-ASLNSPLAG 367

Query: 1080 KQXXXXXXXXXXXXXXXXXKTRGARVIVFDISFDRAKSLADAVCGEVKPYDDLPNFQPEK 1259
            KQ                 K+RGARVI+FDI  DRAKSLA  V GE + +D L +FQPEK
Sbjct: 368  KQFVVVGAGGAGRAIAVGAKSRGARVIIFDIDLDRAKSLAQVVSGEAQHFDSLAHFQPEK 427

Query: 1260 GAILANATPIGMHPNTDRIPVAKETLKDYKLVFDAVYTPRKTTLLRDAESAGALIVSGVE 1439
            GAILANATPIGMHP+TDRIPVA+ TL +Y+LVFDAVYTPRKT LL DA++AGA+ VSGVE
Sbjct: 428  GAILANATPIGMHPSTDRIPVAEATLGNYQLVFDAVYTPRKTRLLEDADAAGAITVSGVE 487

Query: 1440 MFLRQAMGQFNLFTGGQAPEKFMRAIVMEKF 1532
            MFL+QA+GQF+LFTG +AP+ FMR IV+ KF
Sbjct: 488  MFLKQAIGQFSLFTGREAPKDFMREIVLAKF 518


>ref|XP_002328402.1| dehydroquinate dehydratase/ shikimate dehydrogenase [Populus
            trichocarpa] gi|222838117|gb|EEE76482.1| dehydroquinate
            dehydratase/ shikimate dehydrogenase [Populus
            trichocarpa]
          Length = 518

 Score =  656 bits (1693), Expect = 0.0
 Identities = 329/511 (64%), Positives = 404/511 (79%), Gaps = 1/511 (0%)
 Frame = +3

Query: 3    NTTTVCALLMSQSVEQMVSDMYKAKAQGADIVELRLDCLKSFSFPQDLQLLLTNKPLPVI 182
            N+  VCA LM+QSVEQMV DM+ AKAQGAD+VE+RLDC+  F   QDL+ ++ NKPLPVI
Sbjct: 9    NSVMVCAPLMAQSVEQMVIDMHSAKAQGADVVEVRLDCISKFQPRQDLETIIRNKPLPVI 68

Query: 183  IVCRPTSEGGEYEGDENTRIEVLRTAKDLGADYIDIELKVAFSLMEEQKFSQHGGSKIIV 362
            IV RP  EGG+YEGDE+ R+E LR A DLGADYID+ELKVA  L+ E K     G K+IV
Sbjct: 69   IVYRPKWEGGQYEGDEHRRLEALRLANDLGADYIDLELKVASELIWELKNKHQNGGKVIV 128

Query: 363  SCFLDTVTYAKEDLSQVVAHIQSTGADMIKLVSYTSSITDLSKTFHLLSHSQVPLIAYTT 542
            S +L+  T +KE+LS +VA +Q+T AD+IK+VS    IT++ + FHLLSH +VP +AY+ 
Sbjct: 129  SSYLNGATPSKENLSHLVATMQATEADIIKVVSNADDITEMERIFHLLSHCEVPAVAYSV 188

Query: 543  GERGLITQLVGPKFGGHFVYGSVDGNQVLGLPTLYSLRQAYGVEHVDSDTKIFGLISKPV 722
            GERGLI+QL+ PKFGG  VYGS++GN + GLPTL SLR+AY V+ ++SDTK+FGL+SKPV
Sbjct: 189  GERGLISQLLCPKFGGALVYGSMEGNSIPGLPTLDSLREAYKVDCINSDTKVFGLVSKPV 248

Query: 723  SHSKGPILHNPTFRYVGYNGIYVPMFVDDLKDFFRVYSS-DFAGYSVGIPYKEAIIEFCD 899
             HSKGP+LHNPT R+V +NGIYVPMFVDDLK FF VY+S DFAGYSVG PYKEA+++FCD
Sbjct: 249  GHSKGPLLHNPTLRHVNFNGIYVPMFVDDLKKFFDVYASPDFAGYSVGFPYKEAVVQFCD 308

Query: 900  EVHPLAQSIGAVNTIVRRSSDGKLVGYNTDCEASITAIEDTIKVLGCVNGEALLPSPITG 1079
            EVHPLA+ IGAVNTI+RR  DGKL+GYNTDCE SITAIED ++    VNG + L SP+ G
Sbjct: 309  EVHPLAKCIGAVNTIIRRPCDGKLIGYNTDCEGSITAIEDALRDQKYVNGRS-LNSPLAG 367

Query: 1080 KQXXXXXXXXXXXXXXXXXKTRGARVIVFDISFDRAKSLADAVCGEVKPYDDLPNFQPEK 1259
            KQ                 K+RGAR+I+FDI  +RAKSLA AV GE + ++ L +FQPE 
Sbjct: 368  KQFVVVGAGGAGRAIAVGAKSRGARLIIFDIDLERAKSLARAVSGEAQHFESLAHFQPEN 427

Query: 1260 GAILANATPIGMHPNTDRIPVAKETLKDYKLVFDAVYTPRKTTLLRDAESAGALIVSGVE 1439
            GAILANATPIGMHP+TDRIP A+ETL +Y+LVFDAVYTPRKT LL+DA++AGA+ VSGVE
Sbjct: 428  GAILANATPIGMHPSTDRIPAAEETLGNYQLVFDAVYTPRKTRLLKDADAAGAITVSGVE 487

Query: 1440 MFLRQAMGQFNLFTGGQAPEKFMRAIVMEKF 1532
            MFLRQA+GQFNLFTG +AP+ FMR IV+ KF
Sbjct: 488  MFLRQAIGQFNLFTGREAPKDFMREIVLAKF 518