BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004415
         (1495 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACF93731.1| basic beta-1,3-glucanase [Nicotiana tabacum]           231   4e-58
sp|P23431.1|E13B_NICPL RecName: Full=Glucan endo-1,3-beta-glucos...   231   4e-58
emb|CAA37669.1| glucan endo-1,3-beta-glucosidase [Nicotiana taba...   231   4e-58
sp|P15797.2|E13B_TOBAC RecName: Full=Glucan endo-1,3-beta-glucos...   230   6e-58
sp|P27666.1|E13F_TOBAC RecName: Full=Glucan endo-1,3-beta-glucos...   230   8e-58

>gb|ACF93731.1| basic beta-1,3-glucanase [Nicotiana tabacum]
          Length = 370

 Score =  231 bits (589), Expect = 4e-58
 Identities = 111/227 (48%), Positives = 162/227 (71%)
 Frame = +1

Query: 130 SSPFFSLVTLAFLTMLLIAKLTLTGAATIGVDYGRDGNNLPSPREVVDLYRSQGIGAMRI 309
           ++P  + +TL  L +L+ + + + GA +IGV YG  GNNLP+  EV+ LY+S+ IG +R+
Sbjct: 8   NTPQMAAITLLGL-LLVASSIDIAGAQSIGVCYGMLGNNLPNHWEVIQLYKSRNIGRLRL 66

Query: 310 YWPDPEVLQALKGSGIELVLGVPNTDLQQLASDIPSAQRWVRTNVLNYYPDVNIKYISVG 489
           Y P+   LQALKGS IE++LG+PN+D++ +AS +  A+ WV+ NV +++PDV IKYI+VG
Sbjct: 67  YDPNHGALQALKGSNIEVMLGLPNSDVKHIASGMEHARWWVQKNVKDFWPDVKIKYIAVG 126

Query: 490 NEVTPVNPQSRYATFVVPAMTNIFNAVKDAGLFQKVRVSTTLDTTVIDEKTSYPPSAGNF 669
           NE++PV   S   +F+ PAM NI+ A+ +AGL   ++VST++D T+I    SYPPS G+F
Sbjct: 127 NEISPVTGTSYLTSFLTPAMVNIYKAIGEAGLGNNIKVSTSVDMTLIG--NSYPPSQGSF 184

Query: 670 RRDVMWFMNPIIGFLTDTNQPILFTVYPYYAYINNPNTIKLPYVLFT 810
           R D  WF++PI+GFL DT  P+L  +YPY++Y  NP  I LPY LFT
Sbjct: 185 RNDARWFVDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYSLFT 231



 Score = 75.1 bits (183), Expect = 4e-11
 Identities = 35/62 (56%), Positives = 49/62 (79%), Gaps = 1/62 (1%)
 Frame = +2

Query: 1079 EDACTYYQNLVKHVTSVGTPKRPGPIETYLFAMFNE-DKKPENERNFGLFGLNKQPRCAI 1255
            ++A TY +NL++H    G+P++PGPIETY+FAMF+E +K PE E++FGLF  NKQP+  I
Sbjct: 287  DNAATYLRNLIQHAKE-GSPRKPGPIETYIFAMFDENNKNPELEKHFGLFSPNKQPKYNI 345

Query: 1256 NF 1261
            NF
Sbjct: 346  NF 347


>sp|P23431.1|E13B_NICPL RecName: Full=Glucan endo-1,3-beta-glucosidase, basic vacuolar
           isoform; AltName: Full=(1->3)-beta-glucan endohydrolase;
           Short=(1->3)-beta-glucanase; AltName:
           Full=Beta-1,3-endoglucanase, basic; Flags: Precursor
           gi|19687|emb|CAA38540.1| precusor b-1,3-glucanse
           [Nicotiana plumbaginifolia]
          Length = 365

 Score =  231 bits (589), Expect = 4e-58
 Identities = 111/227 (48%), Positives = 162/227 (71%)
 Frame = +1

Query: 130 SSPFFSLVTLAFLTMLLIAKLTLTGAATIGVDYGRDGNNLPSPREVVDLYRSQGIGAMRI 309
           ++P  + +TL  L +L+ + + + GA +IGV YG  GNNLP+  EV+ LY+S+ IG +R+
Sbjct: 8   NTPQMAAITLLGL-LLVASSIEIAGAESIGVCYGMLGNNLPNHWEVIQLYKSRNIGRLRL 66

Query: 310 YWPDPEVLQALKGSGIELVLGVPNTDLQQLASDIPSAQRWVRTNVLNYYPDVNIKYISVG 489
           Y P+   LQALKGS IE++LG+PN+D++ +AS +  A+ WV+ NV +++PDV IKYI+VG
Sbjct: 67  YDPNHGALQALKGSNIEVMLGLPNSDVKHIASGMEHARWWVQKNVKDFWPDVKIKYIAVG 126

Query: 490 NEVTPVNPQSRYATFVVPAMTNIFNAVKDAGLFQKVRVSTTLDTTVIDEKTSYPPSAGNF 669
           NE++PV   S   +F+ PAM NI+ A+ +AGL   ++VST++D T+I    SYPPS G+F
Sbjct: 127 NEISPVTGTSYLTSFLTPAMVNIYKAIGEAGLGNNIKVSTSVDMTLIG--NSYPPSQGSF 184

Query: 670 RRDVMWFMNPIIGFLTDTNQPILFTVYPYYAYINNPNTIKLPYVLFT 810
           R D  WF++PI+GFL DT  P+L  +YPY++Y  NP  I LPY LFT
Sbjct: 185 RNDARWFVDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYSLFT 231



 Score = 71.6 bits (174), Expect = 5e-10
 Identities = 38/74 (51%), Positives = 53/74 (71%), Gaps = 1/74 (1%)
 Frame = +2

Query: 1079 EDACTYYQNLVKHVTSVGTPKRPGPIETYLFAMFNE-DKKPENERNFGLFGLNKQPRCAI 1255
            ++A TY +NL++H    G+P++P PIETY+FAMF+E +K PE E++FGLF  NKQP+  +
Sbjct: 287  DNAATYLKNLIQHAKE-GSPRKPRPIETYIFAMFDENNKNPELEKHFGLFSPNKQPKYNL 345

Query: 1256 NFHGT*CYVRTPAT 1297
            NF G    V T AT
Sbjct: 346  NF-GVSGSVETNAT 358


>emb|CAA37669.1| glucan endo-1,3-beta-glucosidase [Nicotiana tabacum]
           gi|170247|gb|AAA63539.1| glucan beta-1,3-glucanase
           [Nicotiana tabacum]
          Length = 370

 Score =  231 bits (589), Expect = 4e-58
 Identities = 111/227 (48%), Positives = 162/227 (71%)
 Frame = +1

Query: 130 SSPFFSLVTLAFLTMLLIAKLTLTGAATIGVDYGRDGNNLPSPREVVDLYRSQGIGAMRI 309
           ++P  + +TL  L +L+ + + + GA +IGV YG  GNNLP+  EV+ LY+S+ IG +R+
Sbjct: 8   NTPQMAAITLLGL-LLVASSIDIAGAQSIGVCYGMLGNNLPNHWEVIQLYKSRNIGRLRL 66

Query: 310 YWPDPEVLQALKGSGIELVLGVPNTDLQQLASDIPSAQRWVRTNVLNYYPDVNIKYISVG 489
           Y P+   LQALKGS IE++LG+PN+D++ +AS +  A+ WV+ NV +++PDV IKYI+VG
Sbjct: 67  YDPNHGALQALKGSNIEVMLGLPNSDVKHIASGMEHARWWVQKNVKDFWPDVKIKYIAVG 126

Query: 490 NEVTPVNPQSRYATFVVPAMTNIFNAVKDAGLFQKVRVSTTLDTTVIDEKTSYPPSAGNF 669
           NE++PV   S   +F+ PAM NI+ A+ +AGL   ++VST++D T+I    SYPPS G+F
Sbjct: 127 NEISPVTGTSYLTSFLTPAMVNIYKAIGEAGLGNNIKVSTSVDMTLIG--NSYPPSQGSF 184

Query: 670 RRDVMWFMNPIIGFLTDTNQPILFTVYPYYAYINNPNTIKLPYVLFT 810
           R D  WF++PI+GFL DT  P+L  +YPY++Y  NP  I LPY LFT
Sbjct: 185 RNDARWFVDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYSLFT 231



 Score = 75.1 bits (183), Expect = 4e-11
 Identities = 35/62 (56%), Positives = 49/62 (79%), Gaps = 1/62 (1%)
 Frame = +2

Query: 1079 EDACTYYQNLVKHVTSVGTPKRPGPIETYLFAMFNE-DKKPENERNFGLFGLNKQPRCAI 1255
            ++A TY +NL++H    G+P++PGPIETY+FAMF+E +K PE E++FGLF  NKQP+  I
Sbjct: 287  DNAATYLRNLIQHAKE-GSPRKPGPIETYIFAMFDENNKNPELEKHFGLFSPNKQPKYNI 345

Query: 1256 NF 1261
            NF
Sbjct: 346  NF 347


>sp|P15797.2|E13B_TOBAC RecName: Full=Glucan endo-1,3-beta-glucosidase, basic vacuolar
           isoform; AltName: Full=(1->3)-beta-glucan endohydrolase;
           Short=(1->3)-beta-glucanase; AltName:
           Full=Beta-1,3-endoglucanase, basic; Flags: Precursor
          Length = 371

 Score =  230 bits (587), Expect = 6e-58
 Identities = 111/227 (48%), Positives = 161/227 (70%)
 Frame = +1

Query: 130 SSPFFSLVTLAFLTMLLIAKLTLTGAATIGVDYGRDGNNLPSPREVVDLYRSQGIGAMRI 309
           ++P  + +TL  L +L+ + + + GA +IGV YG  GNNLP+  EV+ LY+S+ IG +R+
Sbjct: 9   NTPQMAAITLLGL-LLVASSIDIAGAQSIGVCYGMLGNNLPNHWEVIQLYKSRNIGRLRL 67

Query: 310 YWPDPEVLQALKGSGIELVLGVPNTDLQQLASDIPSAQRWVRTNVLNYYPDVNIKYISVG 489
           Y P+   LQALKGS IE++LG+PN+D++ +AS +  A+ WV+ NV +++PDV IKYI+VG
Sbjct: 68  YDPNHGALQALKGSNIEVMLGLPNSDVKHIASGMEHARWWVQKNVKDFWPDVKIKYIAVG 127

Query: 490 NEVTPVNPQSRYATFVVPAMTNIFNAVKDAGLFQKVRVSTTLDTTVIDEKTSYPPSAGNF 669
           NE++PV   S   +F+ PAM NI+ A+ +AGL   ++VST++D T+I    SYPPS G+F
Sbjct: 128 NEISPVTGTSYLTSFLTPAMVNIYKAIGEAGLGNNIKVSTSVDMTLIG--NSYPPSQGSF 185

Query: 670 RRDVMWFMNPIIGFLTDTNQPILFTVYPYYAYINNPNTIKLPYVLFT 810
           R D  WF +PI+GFL DT  P+L  +YPY++Y  NP  I LPY LFT
Sbjct: 186 RNDARWFTDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYSLFT 232



 Score = 75.1 bits (183), Expect = 4e-11
 Identities = 35/62 (56%), Positives = 49/62 (79%), Gaps = 1/62 (1%)
 Frame = +2

Query: 1079 EDACTYYQNLVKHVTSVGTPKRPGPIETYLFAMFNE-DKKPENERNFGLFGLNKQPRCAI 1255
            ++A TY +NL++H    G+P++PGPIETY+FAMF+E +K PE E++FGLF  NKQP+  I
Sbjct: 288  DNAATYLRNLIQHAKE-GSPRKPGPIETYIFAMFDENNKNPELEKHFGLFSPNKQPKYNI 346

Query: 1256 NF 1261
            NF
Sbjct: 347  NF 348


>sp|P27666.1|E13F_TOBAC RecName: Full=Glucan endo-1,3-beta-glucosidase, basic vacuolar
           isoform GLB; AltName: Full=(1->3)-beta-glucan
           endohydrolase; Short=(1->3)-beta-glucanase; AltName:
           Full=Beta-1,3-endoglucanase, basic; AltName:
           Full=Glucanase GLB; Flags: Precursor
           gi|170249|gb|AAA63540.1| glucan-1,3-beta-glucosidase
           [Nicotiana tabacum]
          Length = 370

 Score =  230 bits (586), Expect = 8e-58
 Identities = 111/227 (48%), Positives = 161/227 (70%)
 Frame = +1

Query: 130 SSPFFSLVTLAFLTMLLIAKLTLTGAATIGVDYGRDGNNLPSPREVVDLYRSQGIGAMRI 309
           ++P  + +TL  L +L+ + + + GA +IGV YG  GNNLP+  EV+ LY+S+ IG +R+
Sbjct: 8   NTPQMAAITLLGL-LLVASTIEIAGAQSIGVCYGMLGNNLPNHWEVIQLYKSRNIGRLRL 66

Query: 310 YWPDPEVLQALKGSGIELVLGVPNTDLQQLASDIPSAQRWVRTNVLNYYPDVNIKYISVG 489
           Y P+   LQALKGS IE++LG+PN+D++ +AS +  A+ WV+ NV +++PDV IKYI+VG
Sbjct: 67  YDPNHGALQALKGSNIEVMLGLPNSDVKHIASGMEHARWWVQKNVKDFWPDVKIKYIAVG 126

Query: 490 NEVTPVNPQSRYATFVVPAMTNIFNAVKDAGLFQKVRVSTTLDTTVIDEKTSYPPSAGNF 669
           NE++PV   S   +F+ PAM NI+ A+ +AGL   ++VST++D T+I    SYPPS G+F
Sbjct: 127 NEISPVTGTSYLTSFLTPAMVNIYKAIGEAGLGNNIKVSTSVDMTLIG--NSYPPSQGSF 184

Query: 670 RRDVMWFMNPIIGFLTDTNQPILFTVYPYYAYINNPNTIKLPYVLFT 810
           R D  WF +PI+GFL DT  P+L  +YPY++Y  NP  I LPY LFT
Sbjct: 185 RNDARWFTDPIVGFLRDTRAPLLVNIYPYFSYSGNPGQISLPYSLFT 231



 Score = 74.3 bits (181), Expect = 8e-11
 Identities = 34/62 (54%), Positives = 49/62 (79%), Gaps = 1/62 (1%)
 Frame = +2

Query: 1079 EDACTYYQNLVKHVTSVGTPKRPGPIETYLFAMFNE-DKKPENERNFGLFGLNKQPRCAI 1255
            ++A TY +NL++H    G+P++PGPIETY+FAMF+E +K PE E++FGLF  NKQP+  +
Sbjct: 287  DNAATYLRNLIQHAKE-GSPRKPGPIETYIFAMFDENNKNPELEKHFGLFSPNKQPKYNL 345

Query: 1256 NF 1261
            NF
Sbjct: 346  NF 347