BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004308
         (1886 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002274123.2| PREDICTED: probable ubiquitin-conjugating en...   378   e-102
gb|ABK95695.1| unknown [Populus trichocarpa]                          367   5e-99
emb|CAN79081.1| hypothetical protein VITISV_004820 [Vitis vinifera]   365   2e-98
emb|CBI21855.3| unnamed protein product [Vitis vinifera]              352   2e-94
ref|XP_002517911.1| ubiquitin conjugating enzyme, putative [Rici...   346   1e-92

>ref|XP_002274123.2| PREDICTED: probable ubiquitin-conjugating enzyme E2 26-like [Vitis
            vinifera]
          Length = 511

 Score =  378 bits (971), Expect = e-102
 Identities = 187/307 (60%), Positives = 227/307 (73%), Gaps = 1/307 (0%)
 Frame = -2

Query: 1849 EYLNMQAQFDHVDLPPGVEASVSWMKDTTSSMNVSNKDSMVPPHVPGIEEETSFAVS-SD 1673
            +YL +QAQFD+VD+PPGVEASV W+KD T S  +       P   P ++ + +   S SD
Sbjct: 142  DYLKLQAQFDNVDIPPGVEASVPWLKDPTPSEKMPAVVDTSP--TPSLQGQCARVGSFSD 199

Query: 1672 IQKFAHKSIASSSSMLHVGSTSDVKKEAAEEHSDMNNEADKEHDVMKNYECFKRFDTVND 1493
              +   K+ A+SSS +   S             D N +     DV+  Y  FK+FDTV D
Sbjct: 200  PGEKETKTAATSSSTVPAQS------------GDGNQD-----DVLGKYLFFKQFDTVED 242

Query: 1492 FSDHHYSAAGWEGQQPPKTWTKKIHDEWKMLEKGLPDTIYVRVYETRMDLLRAIIIGPAG 1313
            FSDHH+S  G+ G+QPPK W KKI +EWK+LE+ LP+TI+VRVYE RMDLLRA+I+GPAG
Sbjct: 243  FSDHHFSRMGFVGEQPPKNWAKKIQEEWKILERDLPETIFVRVYEARMDLLRAVIVGPAG 302

Query: 1312 TPYHDGLFVFDILFPPTYPDIPPMVYYYSGGLRLNPNLYDCGKVCLSLLNTWSGQGNEKW 1133
            TPYHDGLFVFD+LFPP YP +PPMVYYYSGGLRLNPNLYDCGKVCLSLLNTW+G+  E W
Sbjct: 303  TPYHDGLFVFDVLFPPNYPSVPPMVYYYSGGLRLNPNLYDCGKVCLSLLNTWNGKKTEMW 362

Query: 1132 MPKSSTMLQVLVSIQALILNEEPFFNEPGYDGMYPGEVGRKKSKEYNENTFILSLKTMMY 953
            +P  STMLQVLVSIQ LILN +PFFNEPGY+ MY GE G ++SK+YNE+ FILSLKTM+Y
Sbjct: 363  IPGKSTMLQVLVSIQGLILNAKPFFNEPGYENMYVGEDGERRSKQYNEDVFILSLKTMVY 422

Query: 952  TLRRPPK 932
            T+RRPPK
Sbjct: 423  TIRRPPK 429


>gb|ABK95695.1| unknown [Populus trichocarpa]
          Length = 526

 Score =  367 bits (943), Expect = 5e-99
 Identities = 183/313 (58%), Positives = 220/313 (70%), Gaps = 7/313 (2%)
 Frame = -2

Query: 1849 EYLNMQAQFDHVDLPPGVEASVSWMKDTTSSMNVSNKDSM-------VPPHVPGIEEETS 1691
            +Y  +Q+QFD+VDLPPGVEA++ W+K+  SS N+ +  S        +P H      E+ 
Sbjct: 142  DYSAIQSQFDNVDLPPGVEATLPWLKEPASSSNMISNTSTSTIPNHTIPSHTNHNHSESK 201

Query: 1690 FAVSSDIQKFAHKSIASSSSMLHVGSTSDVKKEAAEEHSDMNNEADKEHDVMKNYECFKR 1511
               ++ + +  +K  AS+SS +            A+  S+   E   E++ M+ Y  FK+
Sbjct: 202  IMTTTCLSE--NKWTASASSSMF----------PADSSSNGEVEDSGENNTMQKYRNFKQ 249

Query: 1510 FDTVNDFSDHHYSAAGWEGQQPPKTWTKKIHDEWKMLEKGLPDTIYVRVYETRMDLLRAI 1331
            FD V DFSDHHY         PPK W K+I DEWK LEK LPDTI+VRVYETRM+LLRA+
Sbjct: 250  FDNVEDFSDHHYRGTSVSDLPPPKIWAKRIQDEWKSLEKDLPDTIFVRVYETRMELLRAV 309

Query: 1330 IIGPAGTPYHDGLFVFDILFPPTYPDIPPMVYYYSGGLRLNPNLYDCGKVCLSLLNTWSG 1151
            I GPAGTPYHDGLFVFD +FPPTYPD PPMVYYYSGGLRLNPNLY+CGKVCLSLL TWSG
Sbjct: 310  IAGPAGTPYHDGLFVFDCIFPPTYPDAPPMVYYYSGGLRLNPNLYECGKVCLSLLGTWSG 369

Query: 1150 QGNEKWMPKSSTMLQVLVSIQALILNEEPFFNEPGYDGMYPGEVGRKKSKEYNENTFILS 971
            +  E W+P  STMLQVLVSIQALILN +PFFNEPGY+  Y G  G K+SK+YNE  FILS
Sbjct: 370  KETEMWIPGKSTMLQVLVSIQALILNAKPFFNEPGYESSYVGAEGDKRSKKYNEEVFILS 429

Query: 970  LKTMMYTLRRPPK 932
            LKTMMYTLRRPPK
Sbjct: 430  LKTMMYTLRRPPK 442


>emb|CAN79081.1| hypothetical protein VITISV_004820 [Vitis vinifera]
          Length = 532

 Score =  365 bits (937), Expect = 2e-98
 Identities = 186/328 (56%), Positives = 227/328 (69%), Gaps = 22/328 (6%)
 Frame = -2

Query: 1849 EYLNMQAQFDHVDLPPGVEASVSWMKDTTSSMNVSNKDSMVPPHVPGIEEETSFAVS-SD 1673
            +YL +QAQFD+VD+PPGV+ASV W+KD T S  +       P   P ++ + +   S SD
Sbjct: 142  DYLKLQAQFDNVDIPPGVZASVPWLKDPTPSEKMPAVVDTSP--TPSLQGQCAPVGSFSD 199

Query: 1672 IQKFAHKSIASSSSMLHVGSTSDVKKEAAEEHSDMNNEADKEHDVMKNYECFKRFDTVND 1493
              +   K+ A+SSS +   S             D N +     DV+  Y  FK+FDTV D
Sbjct: 200  PGEKETKTAATSSSTVPAQS------------GDGNQD-----DVLGKYLFFKQFDTVED 242

Query: 1492 FSDHHYSAAGWEGQQPPKTWTKKIHDEWKMLEKGLP---------------------DTI 1376
            FSDHH+S  G+ G+QPPK W KKI +EWK+LE+ LP                     +TI
Sbjct: 243  FSDHHFSRMGFVGEQPPKNWAKKIQEEWKILERDLPGKYRLKEGGWCTKEVRQGYGVETI 302

Query: 1375 YVRVYETRMDLLRAIIIGPAGTPYHDGLFVFDILFPPTYPDIPPMVYYYSGGLRLNPNLY 1196
            +VRVYE RMDLLRA+I+GPAGTPYHDGLFVFD+LFPP YP +PPMVYYYSGGLRLNPNLY
Sbjct: 303  FVRVYEARMDLLRAVIVGPAGTPYHDGLFVFDVLFPPNYPSVPPMVYYYSGGLRLNPNLY 362

Query: 1195 DCGKVCLSLLNTWSGQGNEKWMPKSSTMLQVLVSIQALILNEEPFFNEPGYDGMYPGEVG 1016
            DCGKVCLSLLNTW+G+  E W+P  STMLQVLVSIQ LILN +PFFNEPGY+ MY GE G
Sbjct: 363  DCGKVCLSLLNTWNGKKTEMWIPGKSTMLQVLVSIQGLILNAKPFFNEPGYENMYVGEDG 422

Query: 1015 RKKSKEYNENTFILSLKTMMYTLRRPPK 932
             ++SK+YNE+ FILSLKTM+YT+RRPPK
Sbjct: 423  ERRSKQYNEDVFILSLKTMVYTIRRPPK 450


>emb|CBI21855.3| unnamed protein product [Vitis vinifera]
          Length = 472

 Score =  352 bits (904), Expect = 2e-94
 Identities = 176/306 (57%), Positives = 209/306 (68%)
 Frame = -2

Query: 1849 EYLNMQAQFDHVDLPPGVEASVSWMKDTTSSMNVSNKDSMVPPHVPGIEEETSFAVSSDI 1670
            +YL +QAQFD+VD+PPGVEAS                       VP +++ T        
Sbjct: 142  DYLKLQAQFDNVDIPPGVEAS-----------------------VPWLKDPTP------- 171

Query: 1669 QKFAHKSIASSSSMLHVGSTSDVKKEAAEEHSDMNNEADKEHDVMKNYECFKRFDTVNDF 1490
                      S  M  V  TS                    +DV+  Y  FK+FDTV DF
Sbjct: 172  ----------SEKMPAVVDTSPTP-----------------NDVLGKYLFFKQFDTVEDF 204

Query: 1489 SDHHYSAAGWEGQQPPKTWTKKIHDEWKMLEKGLPDTIYVRVYETRMDLLRAIIIGPAGT 1310
            SDHH+S  G+ G+QPPK W KKI +EWK+LE+ LP+TI+VRVYE RMDLLRA+I+GPAGT
Sbjct: 205  SDHHFSRMGFVGEQPPKNWAKKIQEEWKILERDLPETIFVRVYEARMDLLRAVIVGPAGT 264

Query: 1309 PYHDGLFVFDILFPPTYPDIPPMVYYYSGGLRLNPNLYDCGKVCLSLLNTWSGQGNEKWM 1130
            PYHDGLFVFD+LFPP YP +PPMVYYYSGGLRLNPNLYDCGKVCLSLLNTW+G+  E W+
Sbjct: 265  PYHDGLFVFDVLFPPNYPSVPPMVYYYSGGLRLNPNLYDCGKVCLSLLNTWNGKKTEMWI 324

Query: 1129 PKSSTMLQVLVSIQALILNEEPFFNEPGYDGMYPGEVGRKKSKEYNENTFILSLKTMMYT 950
            P  STMLQVLVSIQ LILN +PFFNEPGY+ MY GE G ++SK+YNE+ FILSLKTM+YT
Sbjct: 325  PGKSTMLQVLVSIQGLILNAKPFFNEPGYENMYVGEDGERRSKQYNEDVFILSLKTMVYT 384

Query: 949  LRRPPK 932
            +RRPPK
Sbjct: 385  IRRPPK 390


>ref|XP_002517911.1| ubiquitin conjugating enzyme, putative [Ricinus communis]
            gi|223542893|gb|EEF44429.1| ubiquitin conjugating enzyme,
            putative [Ricinus communis]
          Length = 521

 Score =  346 bits (888), Expect = 1e-92
 Identities = 179/310 (57%), Positives = 213/310 (68%), Gaps = 4/310 (1%)
 Frame = -2

Query: 1849 EYLNMQAQFDHVDLPPGVEASVSWMKDTTSSMNVSNKDSMVPPHVPGIEEETSFAVSSDI 1670
            +YL  Q+QFD+VDLP GVEAS+ W+K+  +S            + PG    T    S   
Sbjct: 131  DYLAYQSQFDNVDLPAGVEASLPWLKEPANSGF----------NTPGTSTSTMSDPSESK 180

Query: 1669 QKFAHKSIASSSSMLHVGSTSDVKKEAAEEHSDMNNEA--DKEHDVMKNYECFKRFDTVN 1496
            +K     IA +         S+    AA   S  N E   +K   V+   + FK+FDTV 
Sbjct: 181  RKSVILDIAETKRKATGSGLSENNSSAATTASSSNGEEIDNKRKGVLHILKNFKQFDTVE 240

Query: 1495 DFSDHHYSAAGWEGQQ--PPKTWTKKIHDEWKMLEKGLPDTIYVRVYETRMDLLRAIIIG 1322
            DFSDHHY       +Q   P+ W K+I +EWK+LEK LPDTI VRVYE+RM+LLRA+I+G
Sbjct: 241  DFSDHHYCRMESSDKQRPDPRGWAKRIQEEWKILEKDLPDTISVRVYESRMELLRAVIVG 300

Query: 1321 PAGTPYHDGLFVFDILFPPTYPDIPPMVYYYSGGLRLNPNLYDCGKVCLSLLNTWSGQGN 1142
            PAGTPYHDGLFVFD LFP TYP+ PPMVYYYSGGLRLNPNLY+CGKVCLSLL TWSG+  
Sbjct: 301  PAGTPYHDGLFVFDCLFPSTYPNAPPMVYYYSGGLRLNPNLYECGKVCLSLLGTWSGKQT 360

Query: 1141 EKWMPKSSTMLQVLVSIQALILNEEPFFNEPGYDGMYPGEVGRKKSKEYNENTFILSLKT 962
            E W+P  STMLQVLVSIQALILN +PFFNEPGY+  Y GE G K+S++YNE+ FILSLKT
Sbjct: 361  EMWIPGRSTMLQVLVSIQALILNAKPFFNEPGYETSYAGESGDKESRKYNEDVFILSLKT 420

Query: 961  MMYTLRRPPK 932
            MMYTLRRPPK
Sbjct: 421  MMYTLRRPPK 430