BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004225
         (2965 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]               924   0.0  
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...   904   0.0  
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   900   0.0  
ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like ...   883   0.0  
ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like ...   882   0.0  

>gb|AAN15318.1| isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score =  924 bits (2389), Expect = 0.0
 Identities = 475/869 (54%), Positives = 600/869 (69%), Gaps = 17/869 (1%)
 Frame = +2

Query: 197  MAAFPVSTIIHQRATESFNPVFAGHITSKYIVAKRSGKHD----GHRNLVFEGVAMSAQN 364
            MA  P+   +H R          G   S  +V   SG         R L  E +  S   
Sbjct: 1    MATSPIQLAVHSRLLSY------GSTESTKLVPSSSGNRGKIVCSLRKLELEDMNFSGIG 54

Query: 365  RSSYK------HRQNVLITSQSYVLHSTEKVWKYLFRSETSDLVKVYVELKNNRYSVYVE 526
            R++ +      HR+  L  S+  ++ S ++V  YLFR++    VKV VE  N +Y V VE
Sbjct: 55   RNNDQEAPRRAHRRKALSASRISLVPSAKRVPTYLFRTDIGGQVKVLVERTNGKYKVLVE 114

Query: 527  VSSPKLSESKGDLLMVWGMYRSDSSSFMPMDYHDPGLTSTQNMTETPLLLKSSGHIAVEL 706
            V   +LS +  +L+MVWG++RSD+S FMP+D +  G     +  ETP +   SG + VEL
Sbjct: 115  VLPLELSYAHSELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVEL 174

Query: 707  NLKANLAPFYLSFVLKSVSRDGSRISKIRSHRNTNFCVPVGFSSGYPAPLGLSLSTDGYM 886
            + +A+LAPFY+SF +KS        S+IRSHRNTNF VPVG SSG+PAPLG+S   DG +
Sbjct: 175  DFEASLAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGSV 234

Query: 887  NFSLFSRSAEKVVLCLYDKTMDDEPALEIDLDPYVNRTGDIWHASINLAIPLVSYGYRCR 1066
            NF+LFSRSA  VVLCLYD    ++P+LEIDLDPY+NR+GDIWHA+++ ++P  +YGYRC+
Sbjct: 235  NFALFSRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRCK 294

Query: 1067 AGTNQNGERVLLDPYAKIVGDINPG-----MFPQYLGQLQTVPDFDWTGDVRLCLPLEKL 1231
            A T+  GE VLLDPYAK++  + P      + P+YLG+L   P +DW+GDV   LP+EKL
Sbjct: 295  ATTSGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKL 354

Query: 1232 VVYRLNVMHFTIDKSSELANDIGGTFSGLTEKLQHFKNLGINTILLEPVFPFDNQQGPYF 1411
            ++YRLNV  FT DKSS+L +D+ GTFSG++EK  HFK+LG+N +LLEP+FPFD Q+GPYF
Sbjct: 355  IIYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYF 414

Query: 1412 PFHFFSPAYIYGHSKSTISSIASLKEMVKTFHANGMEVFMEVVFTHTADIGSLKEIDISS 1591
            P+HFFSP  +YG S   +S+I S+K+MVK  HANG+EVF+EVVFTHTA+   L  +D  S
Sbjct: 415  PWHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFS 474

Query: 1592 YHYVK--EYDGIQKNTLNCNFPVVQHLVLDSLRHWVIEFHIDGFVFMNAASLLRGCNGEK 1765
            Y  +K  +Y  IQ N LNCN+P+VQ ++LD LRHWVIEFHIDGFVF+NA+SLLRG NGE 
Sbjct: 475  Y-CIKGGQYLNIQ-NALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVNASSLLRGFNGEI 532

Query: 1766 LLRPPLVEAIAFDPILSNVKLIADYWDPHNAEPKAVRFPHWKRWAEVNTNFCEDVRNYLR 1945
            L RPPLVEAIAFDPILS VK+IAD W+P   + K   FPHW+RWAE+N  FC+D+R++LR
Sbjct: 533  LSRPPLVEAIAFDPILSKVKMIADNWNPLTNDSKENLFPHWRRWAEINMRFCDDIRDFLR 592

Query: 1946 GENLLSNLATRLCGSGDKFSAGRGPAFSFNYIARNSGLSLVDLVSFXXXXXXXXXXWNCG 2125
            GE LLSNLATRLCGSGD F+ GRGPAFSFNYIARNSGL+LVDLVSF          WNCG
Sbjct: 593  GEGLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVSFSSNEVASELSWNCG 652

Query: 2126 VEGATTDPATLETRLKQVRNFLFILFVSLGVPVLNMGDECGQSSGGSPAYADRKPFSWNL 2305
             EGATT+   LE RLKQVRNFLFILF+SLGVPVLNMGDECGQSSGG PAY  RK   WN 
Sbjct: 653  QEGATTNNIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGPPAYDARKSLGWNT 712

Query: 2306 LKTSFGIQTTEYIXXXXXXXXXXXXXXQKINFLKEENIEWCGTDLNPPSWEDPTSKFLGM 2485
            LKT FG Q  ++I              QK  FLKEENI+W G+D +PP W+ P+SKFL M
Sbjct: 713  LKTGFGTQIAQFISFLSNLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDGPSSKFLAM 772

Query: 2486 ILKVCKEELQSTSSAPLLYGNMFIAFNAADHSESTVLPSLPANMEWLRLVDTSLSYPVFF 2665
             LK   E  Q+  S   + G++F+AFN A  SE  +LP  P +M W RLVDT+L +P FF
Sbjct: 773  TLKADAEVSQTLVSD--IVGDLFVAFNGAGDSEIVILPPPPTDMVWHRLVDTALPFPGFF 830

Query: 2666 SVDGDPVFDQMPKLVYEMKSHSCILFESR 2752
               G PV D++  + YEMKSHSC+LFE++
Sbjct: 831  DEKGTPVEDEL--VAYEMKSHSCLLFEAQ 857


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
            isoamylase, putative [Ricinus communis]
          Length = 872

 Score =  904 bits (2335), Expect = 0.0
 Identities = 456/800 (57%), Positives = 563/800 (70%), Gaps = 11/800 (1%)
 Frame = +2

Query: 383  RQNVLITSQSYVLHSTEKVWKYLFRSETSDLVKVYVELKNNRYSVYVEVSSPKLSESKGD 562
            +Q  L  S S  L    KV  YLFR++    VKV V  KN +Y+VY+EVSS +L  +   
Sbjct: 71   QQTELRFSTSAPLDELNKVSTYLFRTQFGGHVKVLVRKKNAKYAVYIEVSSLELGTTDYR 130

Query: 563  LLMVWGMYRSDSSSFMPMDYHDPGLTSTQNMTETPLLLKSSGHIAVELNLKANLAPFYLS 742
            L+++WG+YRSDSS FMP+D  +          +T L+  S G  A+EL  +    PFYLS
Sbjct: 131  LMLIWGIYRSDSSCFMPLDSQN--FAPNARKMDTALVQNSFGTFALELEFEPKQTPFYLS 188

Query: 743  FVLKSVSRDGSRISKIRSHRNTNFCVPVGFSSGYPAPLGLSLSTDGYMNFSLFSRSAEKV 922
            F+LKS     +   +I++H+N NFCVP+GF+SG P+PLGLS STDG MNF+ FSR+ E +
Sbjct: 189  FLLKSKLNTDASGLEIKNHKNANFCVPIGFNSGDPSPLGLSFSTDGSMNFAFFSRNVEGL 248

Query: 923  VLCLYDKTMDDEPALEIDLDPYVNRTGDIWHASINLAIPLVSYGYRCRAGTNQNG----- 1087
            VLCLYD +  D+PALE+DLDPYVNRTGD+WHAS+  A    SYGYRC+    Q       
Sbjct: 249  VLCLYDDSTTDKPALELDLDPYVNRTGDVWHASLEGAWTFTSYGYRCKGAILQGNTSKVD 308

Query: 1088 -ERVLLDPYAKIVG----DINPGMFPQYLGQLQTVPDFDWTGDVRLCLPLEKLVVYRLNV 1252
             E VLLDPYA+++     D    +  +YLG+L   P F+W  D+R  L +EKL+VYRLNV
Sbjct: 309  MECVLLDPYARVIASSMTDHGSRLSAKYLGRLCEEPAFEWGSDIRPNLAMEKLIVYRLNV 368

Query: 1253 MHFTIDKSSELANDIGGTFSGLTEKLQHFKNLGINTILLEPVFPFDNQQGPYFPFHFFSP 1432
              FT  KS +L +DI GTF+GL EK+ HF+NLG+N +LLEP+FPFD Q+GPYFP+HFFSP
Sbjct: 369  KRFTEHKSGKLYSDIAGTFAGLIEKMDHFRNLGVNAVLLEPIFPFDEQKGPYFPYHFFSP 428

Query: 1433 AYIYGHSKSTISSIASLKEMVKTFHANGMEVFMEVVFTHTADIGSLKEIDISSYHYVKEY 1612
            + IYG S  +IS+I S+KEMVK  HAN +EV +EVVFTHTA+ G+L+ ID  SY+Y K  
Sbjct: 429  SNIYGPSGGSISAITSMKEMVKELHANRIEVLLEVVFTHTAEGGALQGIDDFSYYYTKSS 488

Query: 1613 DGIQKNTLNCNFPVVQHLVLDSLRHWVIEFHIDGFVFMNAASLLRGCNGEKLLRPPLVEA 1792
                +N LNCN+P+VQ ++LDSL+HWV EFHIDGF F+NA++LL G +GE L RPPLVEA
Sbjct: 489  MD-SRNALNCNYPIVQRMILDSLQHWVTEFHIDGFCFINASALLTGFHGEHLSRPPLVEA 547

Query: 1793 IAFDPILSNVKLIADYWDPHNAEPKAVRFPHWKRWAEVNTNFCEDVRNYLRGENLLSNLA 1972
            IAFDPILS  K+IAD W P +  PK   FPHWKRWAE+N  FC DVRN+LRGE+LL +LA
Sbjct: 548  IAFDPILSKTKIIADPWHPEHRIPKETCFPHWKRWAEINPKFCIDVRNFLRGESLLGDLA 607

Query: 1973 TRLCGSGDKFSAGRGPAFSFNYIARNSGLSLVDLVSFXXXXXXXXXXWNCGVEGATTDPA 2152
            TRLCGSGD FS GRGPAFSFNYIARNSGL LVDLVSF          WNCG EG T   A
Sbjct: 608  TRLCGSGDIFSNGRGPAFSFNYIARNSGLPLVDLVSFSGGELGSELSWNCGEEGPTNKTA 667

Query: 2153 TLETRLKQVRNFLFILFVSLGVPVLNMGDECGQSSGGSPAYADRKPFSWNLLKTSFGIQT 2332
             LE RLKQ+RN+LFIL+VSLGVPVLNMGDECGQSS GS +Y DRKPF WN L TSFG Q 
Sbjct: 668  VLERRLKQIRNYLFILYVSLGVPVLNMGDECGQSSRGSISYGDRKPFDWNALSTSFGNQM 727

Query: 2333 TEYIXXXXXXXXXXXXXXQKINFLKEENIEWCGTDLNPPSWEDPTSKFLGMILKVCKEEL 2512
            T++I              QK NFLKEENI+W G D +PP WEDPT KFL M LK+ K E 
Sbjct: 728  TQFISFLSSLRMRRSDLLQKRNFLKEENIDWHGNDQSPPRWEDPTCKFLAMTLKIDKAES 787

Query: 2513 QSTSSAPLLYGNMFIAFNAADHSESTVLPSLPANMEWLRLVDTSLSYPVFFSVDGDPVFD 2692
            Q +S    + G++F+AFNAA H+ES +LP +P  M W RLVDT+L +P FFS DG+PV +
Sbjct: 788  QLSSEPSNIKGDLFMAFNAAGHAESVILPPVPEGMIWRRLVDTALPFPGFFSEDGEPVVE 847

Query: 2693 QMPKLV-YEMKSHSCILFES 2749
            Q+  L+ Y+M SHSC LFE+
Sbjct: 848  QIAGLIAYKMNSHSCTLFEA 867


>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  900 bits (2327), Expect = 0.0
 Identities = 461/812 (56%), Positives = 573/812 (70%), Gaps = 12/812 (1%)
 Frame = +2

Query: 350  MSAQNRSSYKHRQNVLITSQSYVLHSTEKVWKYLFRSETSDLVKVYVELKNNRYSVYVEV 529
            +SA +  + +  +N   T++S  L S      YLF +E    VKV V  KN +Y V +EV
Sbjct: 71   VSATSHIAIEETENRYSTTESEELESR---LNYLFLTEIGGQVKVIVGKKNKKYIVSIEV 127

Query: 530  SSPKLSESKGDLLMVWGMYRSDSSSFMPMDYHDPGLTSTQNMTETPLLLKSSGHIAVELN 709
            SS +L  S   L++ WG++RS+SS FMP+D+ +       N TE P + +SSG  A++L+
Sbjct: 128  SSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIPFMERSSGSFALKLD 187

Query: 710  LKANLAPFYLSFVLKSVSRDGSRISKIRSHRNTNFCVPVGFSSGYPAPLGLSLSTDGYMN 889
             +AN APFYLSF+LKS        S IRSHR TNFC+PVGF  GYPAPLGLS S+DG  N
Sbjct: 188  FEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGLSFSSDGSPN 247

Query: 890  FSLFSRSAEKVVLCLYDKTMDDEPALEIDLDPYVNRTGDIWHASINLAIPLVSYGYRCR- 1066
            F+ FSR+A  VVLCLYD T  D+PALEIDLDPYVNRTGDIWHAS+      VSYGYRC+ 
Sbjct: 248  FAFFSRNAGGVVLCLYDGT-SDKPALEIDLDPYVNRTGDIWHASMESVGSFVSYGYRCKE 306

Query: 1067 -----AGTNQNGERVLLDPYAKIVGDI---NPGMFPQ-YLGQLQTVPDFDWTGDVRLCLP 1219
                 +G   + E V LDPYAK++ +    + G+ PQ  LG+LQ  P F+W  DV   +P
Sbjct: 307  ANLQDSGETLHTEHVHLDPYAKLIRNSFSDDHGLKPQPRLGELQKEPAFNWNDDVHPYIP 366

Query: 1220 LEKLVVYRLNVMHFTIDKSSELANDIGGTFSGLTEKLQHFKNLGINTILLEPVFPFDNQQ 1399
            +EKLVVYRLNVMHFT D+SS++A+D+ GTFSGL EKL HFK+LG+N +LLEP+F FD Q+
Sbjct: 367  MEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLEPIFSFDEQK 426

Query: 1400 GPYFPFHFFSPAYIYGHSKSTISSIASLKEMVKTFHANGMEVFMEVVFTHTADIGSLKEI 1579
            GPYFPFHFFSP  +YG S   +S+I S+KEMVK  HANG+EV +EVVFTHTA+ G+L+ I
Sbjct: 427  GPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVLLEVVFTHTAESGALQGI 486

Query: 1580 DISSYHYVK-EYDGIQKNTLNCNFPVVQHLVLDSLRHWVIEFHIDGFVFMNAASLLRGCN 1756
            D S Y+YV  + D   +N LNCN+ +VQ +++DSLR+WV EFH+DGF F+NA+SLLRG +
Sbjct: 487  DDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEFHVDGFCFINASSLLRGFH 546

Query: 1757 GEKLLRPPLVEAIAFDPILSNVKLIADYWDPHNAEPKAVRFPHWKRWAEVNTNFCEDVRN 1936
            GE L RPPLVE IAFDP+LS  K+IAD WDP N  PK +RFPHWKRWAEVNT FC DVRN
Sbjct: 547  GEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFPHWKRWAEVNTRFCNDVRN 606

Query: 1937 YLRGENLLSNLATRLCGSGDKFSAGRGPAFSFNYIARNSGLSLVDLVSFXXXXXXXXXXW 2116
            +LRGE L S+ ATRLCGSGD F  GRGPAFSFN+  +N GL LVDLVSF          W
Sbjct: 607  FLRGEGL-SDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLPLVDLVSFSSSELASELSW 665

Query: 2117 NCGVEGATTDPATLETRLKQVRNFLFILFVSLGVPVLNMGDECGQSSGGSPAYADRKPFS 2296
            NCG EG T     LE RLKQ+RNFLFIL+VSLGVP+LNMGDECGQSSGGSPAY DRKPF+
Sbjct: 666  NCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPILNMGDECGQSSGGSPAYGDRKPFN 725

Query: 2297 WNLLKTSFGIQTTEYIXXXXXXXXXXXXXXQKINFLKEENIEWCGTDLNPPSWEDPTSKF 2476
            WN +KT FGIQT ++I              Q+ +FLKEE+I+W G+D +PP W+DP+SKF
Sbjct: 726  WNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFLKEESIDWHGSDQSPPRWDDPSSKF 785

Query: 2477 LGMILKVCKEELQSTSSAPLLYGNMFIAFNAADHSESTVLPSLPANMEWLRLVDTSLSYP 2656
            L M LK    E Q  S +  + G++FIAFN AD S   +LP  P  M W RLVDT+L +P
Sbjct: 786  LAMTLKAENMEGQLPSESSSIKGDLFIAFNTADRSVKVILPPPPTGMVWHRLVDTALPFP 845

Query: 2657 VFFSVDGDPVFDQMPKLV-YEMKSHSCILFES 2749
             FF+ DG+ +  +   LV Y+M+SHSC LFE+
Sbjct: 846  GFFTADGEAILKKKSGLVTYKMESHSCALFEA 877


>ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 866

 Score =  883 bits (2282), Expect = 0.0
 Identities = 460/782 (58%), Positives = 555/782 (70%), Gaps = 12/782 (1%)
 Frame = +2

Query: 446  YLFRSETSD-LVKVYVELKNNRYSVYVEVSSPKLSESKGD-LLMVWGMYRSDSSSFMPMD 619
            YLFR+ET   LVKVYV  + +RY VY E+SS  +S S G+ LL+ WG+YRSDSS F+ +D
Sbjct: 88   YLFRTETGGGLVKVYVTKRKDRYFVYTEISSLDISGSVGETLLLCWGVYRSDSSCFVDLD 147

Query: 620  YHDPGLTSTQNMTETPLLLKSSGHIAVELNLKANLAPFYLSFVLKSVSRDGSRISKIRSH 799
                   +   M  +PL+  S G+ AVEL   A   P YLSF L S    G    +IRSH
Sbjct: 148  TIGLRENAATGMNVSPLVQNSDGNFAVELEFDAKHVPLYLSFFLMSSLDAGM---EIRSH 204

Query: 800  RNTNFCVPVGFSSGYPAPLGLSLSTDGYMNFSLFSRSAEKVVLCLYDKTMDDEPALEIDL 979
            R TNFCVPVG   GYP PLGL  S DG +NF++FSR AE VVLCLYD+   ++PALE+DL
Sbjct: 205  RGTNFCVPVGLLPGYPGPLGLFCSPDGSVNFAIFSRRAESVVLCLYDENDMEKPALELDL 264

Query: 980  DPYVNRTGDIWHASINLAIPLVSYGYRCRAGTNQNG------ERVLLDPYAKIVGDINPG 1141
            DPYVNRTGDIWH +   A   +SYGY CR G  +        E V+LDPYAKIVG+  P 
Sbjct: 265  DPYVNRTGDIWHVAFESAKGFMSYGYSCRGGVLKRNKDDGFAEHVVLDPYAKIVGNSYPD 324

Query: 1142 M--FPQYLGQLQTVPDFDWTGDVRLCLPLEKLVVYRLNVMHFTIDKSSELANDIGGTFSG 1315
               F + LG L   PDFDW GD  L L +EKLVVYRLNV  FT  +SS+L + + GTF+G
Sbjct: 325  GVGFVKNLGWLGKEPDFDWGGDCHLDLSMEKLVVYRLNVKRFTQHESSQLPSGLAGTFTG 384

Query: 1316 LTEKLQHFKNLGINTILLEPVFPFDNQQGPYFPFHFFSPAYIYGHSKSTISSIASLKEMV 1495
            L +K+QHFK+LG+N ILLEPVF FD ++GPYFP HFFS  +IYG S   +S+IAS+KEMV
Sbjct: 385  LAKKVQHFKDLGVNAILLEPVFTFDEKKGPYFPSHFFSLMHIYGPSGGPVSAIASMKEMV 444

Query: 1496 KTFHANGMEVFMEVVFTHTADIGSLKEIDISSYHYVKEYDGIQ-KNTLNCNFPVVQHLVL 1672
            KT HANG+EV +EVVF++TA+IG+L+ ID S+Y+Y     G++ ++ LNCN+P+VQ L+L
Sbjct: 445  KTMHANGIEVLVEVVFSNTAEIGALQGIDDSTYYYANGVGGLKGQSALNCNYPIVQSLIL 504

Query: 1673 DSLRHWVIEFHIDGFVFMNAASLLRGCNGEKLLRPPLVEAIAFDPILSNVKLIADYWDPH 1852
            DSLRHWV EFHIDGF F+NA+ LLRG +GE L RPPLVEAIAFDP+LS  K+IAD WDPH
Sbjct: 505  DSLRHWVTEFHIDGFSFLNASHLLRGFHGEYLTRPPLVEAIAFDPVLSKTKIIADCWDPH 564

Query: 1853 NAEPKAVRFPHWKRWAEVNTNFCEDVRNYLRGENLLSNLATRLCGSGDKFSAGRGPAFSF 2032
                K +RFPHW RWAE+NTNFC DVRN+LRGENLLSNLATRLCGSGD FS GRGPAFSF
Sbjct: 565  GMVAKEIRFPHWMRWAEINTNFCNDVRNFLRGENLLSNLATRLCGSGDIFSGGRGPAFSF 624

Query: 2033 NYIARNSGLSLVDLVSFXXXXXXXXXXWNCGVEGATTDPATLETRLKQVRNFLFILFVSL 2212
            NYIARN G+SLVDLVSF          WNCG EG T + A LE RLKQ+RNFLFILFVSL
Sbjct: 625  NYIARNFGVSLVDLVSF---SSTDELSWNCGEEGPTNNTAILERRLKQIRNFLFILFVSL 681

Query: 2213 GVPVLNMGDECGQSSGGSPAYADRKPFSWNLLKTSFGIQTTEYIXXXXXXXXXXXXXXQK 2392
            GVPVLNMGDECGQSSGG  AY   KPFSW+ LKT FG QT+++I              Q+
Sbjct: 682  GVPVLNMGDECGQSSGGFTAYDGIKPFSWSSLKTGFGKQTSQFIFFLSSFRRRRSDLLQR 741

Query: 2393 INFLKEENIEWCGTDLNPPSWEDPTSKFLGMILKVCKEELQSTSSAPLLYGNMFIAFNAA 2572
            ++FLKEENIEW G+D  PP WED + KFL M LK  ++E   +S +  + G++FIAFNAA
Sbjct: 742  MSFLKEENIEWYGSDGAPPRWEDLSCKFLAMALKAEEKEFLESSVSSDISGDLFIAFNAA 801

Query: 2573 DHSESTVLPSLPANMEWLRLVDTSLSYPVFFSVDGDPVFDQMPKL-VYEMKSHSCILFES 2749
             H E+ VLP  P  M W RLVDT+L +P FFS  G+ V +Q   L  Y MKS+SC LFE+
Sbjct: 802  GHPETAVLPLPPEGMLWYRLVDTALPFPGFFSASGEVVPEQTAGLFTYRMKSYSCTLFEA 861

Query: 2750 RH 2755
             +
Sbjct: 862  NN 863


>ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 865

 Score =  882 bits (2279), Expect = 0.0
 Identities = 455/783 (58%), Positives = 558/783 (71%), Gaps = 12/783 (1%)
 Frame = +2

Query: 446  YLFRSETSD-LVKVYVELKNNRYSVYVEVSSPKLSESKGD-LLMVWGMYRSDSSSFMPMD 619
            YLFR+ET   LVKV+V  +N+RYSVY+E+SS  +S   G+ LL+ WG+YRSDSS F+ +D
Sbjct: 87   YLFRTETGGGLVKVHVTRRNDRYSVYIEISSLDISGGVGEALLLCWGVYRSDSSCFVDLD 146

Query: 620  YHDPGLTSTQNMTETPLLLKSSGHIAVELNLKANLAPFYLSFVLKSVSRDGSRISKIRSH 799
                   +   M  +PL+  S G  A+EL   A   P YLSF L S    G    +IRSH
Sbjct: 147  TIGLSENAAMGMNVSPLVQNSDGKFAIELEFDAKHVPLYLSFFLMSSLDSGL---EIRSH 203

Query: 800  RNTNFCVPVGFSSGYPAPLGLSLSTDGYMNFSLFSRSAEKVVLCLYDKTMDDEPALEIDL 979
            R TNFC+PVG   GYP PLG+S S DG +NFS+FSR AE VVLCLYD+   ++PALE+DL
Sbjct: 204  RRTNFCMPVGSLPGYPCPLGVSYSPDGSVNFSIFSRHAESVVLCLYDENGVEKPALELDL 263

Query: 980  DPYVNRTGDIWHASINLAIPLVSYGYRCRAGTNQNG------ERVLLDPYAKIVGDINPG 1141
            DPYVNRTGDIWH S   A   VSYGYRCR G  +        E V+LDPYAKIVG+  P 
Sbjct: 264  DPYVNRTGDIWHVSFESAKGFVSYGYRCRRGVLKKNKDDGFAEHVVLDPYAKIVGNSYPD 323

Query: 1142 M--FPQYLGQLQTVPDFDWTGDVRLCLPLEKLVVYRLNVMHFTIDKSSELANDIGGTFSG 1315
                 + LG L+  P FDW GD    L +EKLVVYRLNV  FT  +SS+L + + GTF+G
Sbjct: 324  GVGLVKNLGCLRKEPFFDWGGDRHPDLSMEKLVVYRLNVKRFTQHESSQLPSGLAGTFTG 383

Query: 1316 LTEKLQHFKNLGINTILLEPVFPFDNQQGPYFPFHFFSPAYIYGHSKSTISSIASLKEMV 1495
            L +K+QHFK+LG+N +LLEPVF FD ++GPYFP HFFS  +IYG S   +S+IA++KEMV
Sbjct: 384  LAKKVQHFKDLGVNAVLLEPVFTFDEKKGPYFPCHFFSLMHIYGPSGGPVSAIAAMKEMV 443

Query: 1496 KTFHANGMEVFMEVVFTHTADIGSLKEIDISSYHYVKEYDGIQ-KNTLNCNFPVVQHLVL 1672
            KT HANG+EV +EVVF++TA+IG+++ ID SSY+Y     G++ ++ LNCN+P+VQ+L+L
Sbjct: 444  KTMHANGIEVLVEVVFSNTAEIGAIQGIDDSSYYYANGVGGLKVQSALNCNYPIVQNLIL 503

Query: 1673 DSLRHWVIEFHIDGFVFMNAASLLRGCNGEKLLRPPLVEAIAFDPILSNVKLIADYWDPH 1852
            DSLRHWV EFHIDGF F+NA+ LLRG +GE L RPPLVEAIAFDP+LS  K+IAD WDPH
Sbjct: 504  DSLRHWVTEFHIDGFSFINASHLLRGFHGEYLSRPPLVEAIAFDPVLSKTKIIADCWDPH 563

Query: 1853 NAEPKAVRFPHWKRWAEVNTNFCEDVRNYLRGENLLSNLATRLCGSGDKFSAGRGPAFSF 2032
                K +RFPHW RWAE+NT+FC DVRN+LRG+NLLS+LATRLCGSGD FS GRGP FSF
Sbjct: 564  GMVAKEIRFPHWMRWAEMNTHFCNDVRNFLRGQNLLSDLATRLCGSGDIFSGGRGPGFSF 623

Query: 2033 NYIARNSGLSLVDLVSFXXXXXXXXXXWNCGVEGATTDPATLETRLKQVRNFLFILFVSL 2212
            NYIARN G+SLVDLVSF          WNCG EG T + A LE RLKQ+RNFLFILFVSL
Sbjct: 624  NYIARNFGVSLVDLVSF---SSVDELSWNCGAEGPTNNTAVLERRLKQIRNFLFILFVSL 680

Query: 2213 GVPVLNMGDECGQSSGGSPAYADRKPFSWNLLKTSFGIQTTEYIXXXXXXXXXXXXXXQK 2392
            GVPVLNMGDECGQSSGG  AY   KPFSW+ LKT FG QT+E+I              Q+
Sbjct: 681  GVPVLNMGDECGQSSGGFTAYDGIKPFSWSALKTGFGKQTSEFIFFLSSLRKRRSYLLQR 740

Query: 2393 INFLKEENIEWCGTDLNPPSWEDPTSKFLGMILKVCKEELQSTSSAPLLYGNMFIAFNAA 2572
             +FLKEENIEW G+D +PP WEDP+ KFL MILK    E   +S +  + G++FIAFNA 
Sbjct: 741  RSFLKEENIEWYGSDGDPPRWEDPSCKFLAMILKAEVTEFLESSVSSDISGDLFIAFNAT 800

Query: 2573 DHSESTVLPSLPANMEWLRLVDTSLSYPVFFSVDGDPVFDQMPKL-VYEMKSHSCILFES 2749
            DH E+ VLP  P  M W RLVDT+L +P FFS  G+ V +Q   L  Y++KS+SC LFE+
Sbjct: 801  DHPETAVLPLPPEGMSWYRLVDTALPFPGFFSTSGEVVPEQTEGLFTYQVKSYSCTLFEA 860

Query: 2750 RHK 2758
             ++
Sbjct: 861  NNR 863