BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004185
         (2010 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002273452.1| PREDICTED: vacuolar cation/proton exchanger ...   709   0.0  
emb|CAN69667.1| hypothetical protein VITISV_001500 [Vitis vinifera]   707   0.0  
dbj|BAA75232.1| H+/Ca2+ exchanger 2 [Ipomoea nil]                     678   0.0  
ref|XP_004148561.1| PREDICTED: vacuolar cation/proton exchanger ...   660   0.0  
ref|XP_002315389.1| Ca2+ antiporter/cation exchanger [Populus tr...   647   0.0  

>ref|XP_002273452.1| PREDICTED: vacuolar cation/proton exchanger 2 [Vitis vinifera]
            gi|298204778|emb|CBI25276.3| unnamed protein product
            [Vitis vinifera]
          Length = 432

 Score =  709 bits (1830), Expect = 0.0
 Identities = 363/433 (83%), Positives = 394/433 (90%)
 Frame = -1

Query: 1791 MGSVEENIDLESDEEIPFSSSQATDKLQNFHFETSRTILEPNISSSWASKRIRNNLIRSI 1612
            MGS+EE IDLESDEEIPFS+SQ T K+ +F FETS       +S+SW  K++R   +RS+
Sbjct: 1    MGSIEEKIDLESDEEIPFSTSQGTRKVHSFDFETSHIGSHAALSTSWIFKKMRTAPLRSV 60

Query: 1611 YIVLIKAKINILLPFGPLAILLHYVTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP 1432
            YIVLIKAKIN+LLPFGPLAI LHY+TGKHGWVFFF+LLGITPLAERLG+ATEQLAFYTG 
Sbjct: 61   YIVLIKAKINMLLPFGPLAIFLHYLTGKHGWVFFFSLLGITPLAERLGFATEQLAFYTGA 120

Query: 1431 TVGGLLNATFGNATEMIISIYALKNGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHYK 1252
            TVGGLLNATFGNATEMIIS+YALK+GMIRVVQQSLLGSILSNMLLVLGCAFFTGGI ++ 
Sbjct: 121  TVGGLLNATFGNATEMIISMYALKHGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIKNHT 180

Query: 1251 KVQLFNKAAALVNSGLLLMAVMGLLFPAVLHFTHTEVHFGKSELALSRFSSCIMLVAYAS 1072
            KVQ FNKAAALVNSGLLLMAVMGL+FPAVLHFTHTEVHFGKSELALSRFSSCIMLVAYAS
Sbjct: 181  KVQEFNKAAALVNSGLLLMAVMGLMFPAVLHFTHTEVHFGKSELALSRFSSCIMLVAYAS 240

Query: 1071 YLFFQLKSQPNLYSAIDNDRECIVEDSEDEEAPEITMWEAIGWLFILTLWVSVLSGYLVD 892
            YLFFQLKSQPNLYS ID D E I EDS DE+ PE+T WEAI WL ILTLWVSVLSGYLVD
Sbjct: 241  YLFFQLKSQPNLYSPIDEDTEKIEEDS-DEDIPELTQWEAISWLAILTLWVSVLSGYLVD 299

Query: 891  AIQGASDSWNMPVSFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFV 712
            AI+GAS+S NMPV+FISVILLPIVGNAAEHASAIMFA+K+KLDIT+GVAIGSSTQI+MFV
Sbjct: 300  AIEGASESLNMPVAFISVILLPIVGNAAEHASAIMFAIKNKLDITIGVAIGSSTQIAMFV 359

Query: 711  IPFCVVVGWFMGQPMDLNFQLFETATLFITVLVVAFMLQDGTSNYFKGLMLILSYLIVAA 532
            IPFCVVVGW MGQPMDLNFQLFETATLFI VLVVAFMLQ+GTSNYFKGLMLIL YLIVAA
Sbjct: 360  IPFCVVVGWIMGQPMDLNFQLFETATLFIAVLVVAFMLQEGTSNYFKGLMLILCYLIVAA 419

Query: 531  SFFVHVDKANEDD 493
            SFFVHVD   ++D
Sbjct: 420  SFFVHVDPTKDND 432


>emb|CAN69667.1| hypothetical protein VITISV_001500 [Vitis vinifera]
          Length = 433

 Score =  707 bits (1826), Expect = 0.0
 Identities = 363/427 (85%), Positives = 392/427 (91%)
 Frame = -1

Query: 1791 MGSVEENIDLESDEEIPFSSSQATDKLQNFHFETSRTILEPNISSSWASKRIRNNLIRSI 1612
            MGS+EE IDLESDEEIPFS+SQ T K+ +F FETS       +S+SW  K++R   +RS+
Sbjct: 1    MGSIEEKIDLESDEEIPFSTSQGTRKVHSFDFETSHIGSHAALSTSWIFKKMRTAPLRSV 60

Query: 1611 YIVLIKAKINILLPFGPLAILLHYVTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTGP 1432
            YIVLIKAKIN+LLPFGPLAILLHY+TGKHGWVFFF+LLGITPLAERLG+ATEQLAFYTG 
Sbjct: 61   YIVLIKAKINMLLPFGPLAILLHYLTGKHGWVFFFSLLGITPLAERLGFATEQLAFYTGA 120

Query: 1431 TVGGLLNATFGNATEMIISIYALKNGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHYK 1252
            TVGGLLNATFGNATEMIIS+YALK+GMIRVVQQSLLGSILSNMLLVLGCAFFTGGI ++ 
Sbjct: 121  TVGGLLNATFGNATEMIISMYALKHGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIKNHT 180

Query: 1251 KVQLFNKAAALVNSGLLLMAVMGLLFPAVLHFTHTEVHFGKSELALSRFSSCIMLVAYAS 1072
            KVQ FNKAAALVNSGLLLMAVMGL+FPAVLHFTHTEVHFGKSELALSRFSSCIMLVAYAS
Sbjct: 181  KVQEFNKAAALVNSGLLLMAVMGLMFPAVLHFTHTEVHFGKSELALSRFSSCIMLVAYAS 240

Query: 1071 YLFFQLKSQPNLYSAIDNDRECIVEDSEDEEAPEITMWEAIGWLFILTLWVSVLSGYLVD 892
            YLFFQLKSQPNLYS ID D E I EDS DE+ PE+T WEAI WL ILTLWVSVLSGYLVD
Sbjct: 241  YLFFQLKSQPNLYSPIDEDTEKIEEDS-DEDIPELTQWEAISWLAILTLWVSVLSGYLVD 299

Query: 891  AIQGASDSWNMPVSFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMFV 712
            AI+GAS+S NMPV+FISVILLPIVGNAAEHASAIMFA+K+KLDIT+GVAIGSSTQI+MFV
Sbjct: 300  AIEGASESLNMPVAFISVILLPIVGNAAEHASAIMFAIKNKLDITIGVAIGSSTQIAMFV 359

Query: 711  IPFCVVVGWFMGQPMDLNFQLFETATLFITVLVVAFMLQDGTSNYFKGLMLILSYLIVAA 532
            IPFCVVVGW MGQPMDLNFQLFETATLFI VLVVAFMLQ+GTSNYFKGLMLIL YLIVAA
Sbjct: 360  IPFCVVVGWIMGQPMDLNFQLFETATLFIAVLVVAFMLQEGTSNYFKGLMLILCYLIVAA 419

Query: 531  SFFVHVD 511
            SFFVHVD
Sbjct: 420  SFFVHVD 426


>dbj|BAA75232.1| H+/Ca2+ exchanger 2 [Ipomoea nil]
          Length = 435

 Score =  678 bits (1750), Expect = 0.0
 Identities = 350/434 (80%), Positives = 388/434 (89%), Gaps = 3/434 (0%)
 Frame = -1

Query: 1791 MGSVEENIDLESDEEIPFSSSQATDKLQNFHFETSRT-ILEPN--ISSSWASKRIRNNLI 1621
            MGS EE+ DLESDE +P SSS    K++  H+E     I  P   +S+    K  + +++
Sbjct: 1    MGSGEESFDLESDE-VPLSSSHGASKMETLHYEAPHNHIASPRAFVSTRLLRKMRQISVL 59

Query: 1620 RSIYIVLIKAKINILLPFGPLAILLHYVTGKHGWVFFFTLLGITPLAERLGYATEQLAFY 1441
            RSIYIVLIKAKIN+LLPFGPLAILLH+VTGKH WVFFF+LLGI PLAERLGYATEQL+FY
Sbjct: 60   RSIYIVLIKAKINVLLPFGPLAILLHHVTGKHAWVFFFSLLGIIPLAERLGYATEQLSFY 119

Query: 1440 TGPTVGGLLNATFGNATEMIISIYALKNGMIRVVQQSLLGSILSNMLLVLGCAFFTGGII 1261
            TGPTVGGLLNATFGNATEMIISIYALKN MIRVV+QSLLGSILSNMLLVLGCAFF+GGII
Sbjct: 120  TGPTVGGLLNATFGNATEMIISIYALKNDMIRVVKQSLLGSILSNMLLVLGCAFFSGGII 179

Query: 1260 HYKKVQLFNKAAALVNSGLLLMAVMGLLFPAVLHFTHTEVHFGKSELALSRFSSCIMLVA 1081
            H+KKVQ+FNKA A+VNSGLLLMAVMGLLFPAVLHFT TEVHFGKSELALSRFSSCIMLVA
Sbjct: 180  HHKKVQVFNKATAIVNSGLLLMAVMGLLFPAVLHFTRTEVHFGKSELALSRFSSCIMLVA 239

Query: 1080 YASYLFFQLKSQPNLYSAIDNDRECIVEDSEDEEAPEITMWEAIGWLFILTLWVSVLSGY 901
            YASYLFFQLKS+ + Y++ID + E   ED ++EEAPEIT WEAIGWL ILT+W+SVLSGY
Sbjct: 240  YASYLFFQLKSERSSYTSIDEEGENNAEDPDEEEAPEITHWEAIGWLAILTIWISVLSGY 299

Query: 900  LVDAIQGASDSWNMPVSFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQIS 721
            LVDAI+GASD+ NMPV+FISVILLPIVGNAAEHASAIMFAMKDKLDI+LGVAIGSSTQIS
Sbjct: 300  LVDAIEGASDTLNMPVAFISVILLPIVGNAAEHASAIMFAMKDKLDISLGVAIGSSTQIS 359

Query: 720  MFVIPFCVVVGWFMGQPMDLNFQLFETATLFITVLVVAFMLQDGTSNYFKGLMLILSYLI 541
            MFVIPFCVVVGWFMG+ MDLNFQLFETATLFITVLVVAFMLQ+GTSNYFKGLMLIL YLI
Sbjct: 360  MFVIPFCVVVGWFMGKDMDLNFQLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLI 419

Query: 540  VAASFFVHVDKANE 499
            VAASFFVH D++N+
Sbjct: 420  VAASFFVHNDQSND 433


>ref|XP_004148561.1| PREDICTED: vacuolar cation/proton exchanger 2-like [Cucumis sativus]
            gi|449508105|ref|XP_004163220.1| PREDICTED: vacuolar
            cation/proton exchanger 2-like [Cucumis sativus]
            gi|256535783|gb|ACU82368.1| cation exchanger 2 [Cucumis
            sativus]
          Length = 427

 Score =  660 bits (1703), Expect = 0.0
 Identities = 344/433 (79%), Positives = 377/433 (87%), Gaps = 1/433 (0%)
 Frame = -1

Query: 1791 MGSVEENIDLESDEEIPFSSSQ-ATDKLQNFHFETSRTILEPNISSSWASKRIRNNLIRS 1615
            MGS EE  +  SDEEIP  SS  A + L +F  ET+     P+ +  W +  IR   +RS
Sbjct: 1    MGSTEEKTNYLSDEEIPLKSSPVAKNVLHSFDCETAAVRSSPD-TRWWKTSNIRIRPLRS 59

Query: 1614 IYIVLIKAKINILLPFGPLAILLHYVTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 1435
             YIVL++AKIN+LLPFGPLAILLHY+TGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG
Sbjct: 60   AYIVLVRAKINVLLPFGPLAILLHYLTGKHGWVFFFTLLGITPLAERLGYATEQLAFYTG 119

Query: 1434 PTVGGLLNATFGNATEMIISIYALKNGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHY 1255
            PTVGGLLNATFGNATEMIISIYALK+GMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIH+
Sbjct: 120  PTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFTGGIIHH 179

Query: 1254 KKVQLFNKAAALVNSGLLLMAVMGLLFPAVLHFTHTEVHFGKSELALSRFSSCIMLVAYA 1075
             KVQ+FNK AA+VNSGLLLMAVMG  FPAVLHFTHTE+H G+S L+LSRFSSCIML+AYA
Sbjct: 180  TKVQVFNKGAAVVNSGLLLMAVMGTTFPAVLHFTHTELHLGESALSLSRFSSCIMLIAYA 239

Query: 1074 SYLFFQLKSQPNLYSAIDNDRECIVEDSEDEEAPEITMWEAIGWLFILTLWVSVLSGYLV 895
            SYLFFQLKSQ +LY  +D +      D E E+  EI  WEAIGWL ILT+WVSVLSGYLV
Sbjct: 240  SYLFFQLKSQHDLYGPLDEE-----VDGEVEDDSEIYAWEAIGWLAILTVWVSVLSGYLV 294

Query: 894  DAIQGASDSWNMPVSFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMF 715
            DAIQGAS+S N+PV+FISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMF
Sbjct: 295  DAIQGASESLNLPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQISMF 354

Query: 714  VIPFCVVVGWFMGQPMDLNFQLFETATLFITVLVVAFMLQDGTSNYFKGLMLILSYLIVA 535
            VIPFCVVVGW MG+PMDLNF+LFETATLFITVLVVAFMLQ+GTSNYFKGLMLIL YLIVA
Sbjct: 355  VIPFCVVVGWCMGKPMDLNFKLFETATLFITVLVVAFMLQEGTSNYFKGLMLILCYLIVA 414

Query: 534  ASFFVHVDKANED 496
            ASFFVHVD +N+D
Sbjct: 415  ASFFVHVDPSNDD 427


>ref|XP_002315389.1| Ca2+ antiporter/cation exchanger [Populus trichocarpa]
            gi|222864429|gb|EEF01560.1| Ca2+ antiporter/cation
            exchanger [Populus trichocarpa]
          Length = 371

 Score =  647 bits (1669), Expect = 0.0
 Identities = 328/371 (88%), Positives = 349/371 (94%)
 Frame = -1

Query: 1623 IRSIYIVLIKAKINILLPFGPLAILLHYVTGKHGWVFFFTLLGITPLAERLGYATEQLAF 1444
            +RSIYIVLIKAKIN+LLPFGPLAILLHY+TGKHG VF  ++LGITPLAERLGY TEQLAF
Sbjct: 1    LRSIYIVLIKAKINMLLPFGPLAILLHYLTGKHGLVFLLSMLGITPLAERLGYVTEQLAF 60

Query: 1443 YTGPTVGGLLNATFGNATEMIISIYALKNGMIRVVQQSLLGSILSNMLLVLGCAFFTGGI 1264
            YTGPTVGGLLNATFGNATEMIISIYALK+GMIRVVQQSLLGSILSNMLLVLGCAFF+GGI
Sbjct: 61   YTGPTVGGLLNATFGNATEMIISIYALKSGMIRVVQQSLLGSILSNMLLVLGCAFFSGGI 120

Query: 1263 IHYKKVQLFNKAAALVNSGLLLMAVMGLLFPAVLHFTHTEVHFGKSELALSRFSSCIMLV 1084
             +++KVQ+FNKAAALVNSGLLLMAVMG++FPAVLHFT TEVHFGKSELALSRFSSCIML+
Sbjct: 121  ANHQKVQVFNKAAALVNSGLLLMAVMGIMFPAVLHFTRTEVHFGKSELALSRFSSCIMLI 180

Query: 1083 AYASYLFFQLKSQPNLYSAIDNDRECIVEDSEDEEAPEITMWEAIGWLFILTLWVSVLSG 904
            AY SYLFFQLKSQPNLYS +D       EDS+DEEAPEIT WE IGWL ILTLW+S+LSG
Sbjct: 181  AYGSYLFFQLKSQPNLYSLVDEGGVNDQEDSDDEEAPEITQWETIGWLAILTLWISILSG 240

Query: 903  YLVDAIQGASDSWNMPVSFISVILLPIVGNAAEHASAIMFAMKDKLDITLGVAIGSSTQI 724
            YLVDAIQGASDS NMPV+FISVILLPIVGNAAEHASAIMFAMKDKLDIT+GVAIGSSTQI
Sbjct: 241  YLVDAIQGASDSLNMPVAFISVILLPIVGNAAEHASAIMFAMKDKLDITIGVAIGSSTQI 300

Query: 723  SMFVIPFCVVVGWFMGQPMDLNFQLFETATLFITVLVVAFMLQDGTSNYFKGLMLILSYL 544
            SMFVIPFCVVVGW MGQPMDLNFQLFETATLFI+VLVVAFMLQDGTSNYFKG MLIL YL
Sbjct: 301  SMFVIPFCVVVGWCMGQPMDLNFQLFETATLFISVLVVAFMLQDGTSNYFKGFMLILCYL 360

Query: 543  IVAASFFVHVD 511
            IVAASFFVHVD
Sbjct: 361  IVAASFFVHVD 371