BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004139
         (2335 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283889.2| PREDICTED: aspartic proteinase nepenthesin-1...   604   e-170
ref|XP_002514026.1| Aspartic proteinase nepenthesin-1 precursor,...   597   e-168
ref|XP_002302336.1| predicted protein [Populus trichocarpa] gi|2...   581   e-163
gb|ABK95290.1| unknown [Populus trichocarpa]                          577   e-162
ref|XP_004148418.1| PREDICTED: aspartic proteinase nepenthesin-1...   563   e-158

>ref|XP_002283889.2| PREDICTED: aspartic proteinase nepenthesin-1-like [Vitis vinifera]
          Length = 439

 Score =  604 bits (1558), Expect = e-170
 Identities = 295/423 (69%), Positives = 347/423 (82%), Gaps = 2/423 (0%)
 Frame = -3

Query: 2105 AFDHACSSAANYK-SDISIVHIYGKCSPFHAPKPTSSWANTVIDMASRDQQRLAYLSSLV 1929
            AFD   S ++  K SD+S++H+YG+CSPF+  K   SW NTVI+MAS+D  R+ YLSSLV
Sbjct: 19   AFDPCASPSSESKGSDLSVIHVYGQCSPFNQHK-AGSWVNTVINMASKDPARVTYLSSLV 77

Query: 1928 GQ-KPTKVPIASGQNVLNIGNYVARVSVGTPGQLMFMVLDTSNDAAWIPCSGCTHCTSTM 1752
               K T VPIASGQ VLNIGNYV RV +GTPGQLMFMVLDTS DAAW+PC+ C  C+S  
Sbjct: 78   ASPKATSVPIASGQQVLNIGNYVVRVKLGTPGQLMFMVLDTSRDAAWVPCADCAGCSSPT 137

Query: 1751 FAPNGSSTYLTMDCSMSECAQVRGLACPASGSSTTCQFNQSYGGDSSFSAILSRDSLGLA 1572
            F+PN SSTY ++ CS+ +C QVRGL+CP +G++  C FNQ+YGGDSSFSA+LS+DSLGLA
Sbjct: 138  FSPNTSSTYASLQCSVPQCTQVRGLSCPTTGTAA-CFFNQTYGGDSSFSAMLSQDSLGLA 196

Query: 1571 NDVIPNYTFGCINDVSGKSIPPQXXXXXGRGPMSLLSQSQSFYSGVFSYCLPSFKSYYFS 1392
             D +P+Y+FGC+N VSG ++PPQ     GRGPMSLLSQS S YSGVFSYC PSFKSYYFS
Sbjct: 197  VDTLPSYSFGCVNAVSGSTLPPQGLLGLGRGPMSLLSQSGSLYSGVFSYCFPSFKSYYFS 256

Query: 1391 GSLKLGPLGQPKNIRTTPLLKNPHRPSLYYVNLTGINVGRISVPIAPELLAFDPNTGAGT 1212
            GSL+LGPLGQPKNIRTTPLL+NPHRP+LYYVNLTG++VGR+ VP+APELLAFDPNTGAGT
Sbjct: 257  GSLRLGPLGQPKNIRTTPLLRNPHRPTLYYVNLTGVSVGRVLVPVAPELLAFDPNTGAGT 316

Query: 1211 IIDSGTVITRFAQPVYNAVRDEFRKQIKGPFSSLGAFDTCFAATNEDIAPAITFRFTGMD 1032
            IIDSGTVITRF +PVY A+RDEFRKQ+KGPF+++GAFDTCFAATNEDIAP +TF FTGMD
Sbjct: 317  IIDSGTVITRFVEPVYAAIRDEFRKQVKGPFATIGAFDTCFAATNEDIAPPVTFHFTGMD 376

Query: 1031 LVLPMENSLIHSSSGTTACLXXXXXXXXXXXXXXXXXNLQQQNLRIMFDIANSRLGIARE 852
            L LP+EN+LIHSS+G+ ACL                 NLQQQNLRIMFD+ NSRLGIARE
Sbjct: 377  LKLPLENTLIHSSAGSLACLAMAAAPNNVNSVLNVIANLQQQNLRIMFDVTNSRLGIARE 436

Query: 851  LCN 843
            LCN
Sbjct: 437  LCN 439


>ref|XP_002514026.1| Aspartic proteinase nepenthesin-1 precursor, putative [Ricinus
            communis] gi|223547112|gb|EEF48609.1| Aspartic proteinase
            nepenthesin-1 precursor, putative [Ricinus communis]
          Length = 437

 Score =  597 bits (1538), Expect = e-168
 Identities = 297/416 (71%), Positives = 337/416 (81%)
 Frame = -3

Query: 2090 CSSAANYKSDISIVHIYGKCSPFHAPKPTSSWANTVIDMASRDQQRLAYLSSLVGQKPTK 1911
            C+S A+  SD+SI+ IY KCSPF  PK      NTVIDMAS+D  RL YLSSL  Q  T 
Sbjct: 25   CASQAD-DSDLSIIPIYSKCSPFIPPKQ-EPLVNTVIDMASKDPARLKYLSSLAAQMTTA 82

Query: 1910 VPIASGQNVLNIGNYVARVSVGTPGQLMFMVLDTSNDAAWIPCSGCTHCTSTMFAPNGSS 1731
            VPIA GQ VLNIGNYV RV +GTPGQ MFMVLDTSNDAAW+PCSGCT C+ST F+ N SS
Sbjct: 83   VPIAPGQQVLNIGNYVVRVKLGTPGQFMFMVLDTSNDAAWVPCSGCTGCSSTTFSTNTSS 142

Query: 1730 TYLTMDCSMSECAQVRGLACPASGSSTTCQFNQSYGGDSSFSAILSRDSLGLANDVIPNY 1551
            TY ++DCSM++C QVRG +CPA+GSS+ C FNQSYGGDSSFSA L  DSL L NDVIPN+
Sbjct: 143  TYGSLDCSMAQCTQVRGFSCPATGSSS-CVFNQSYGGDSSFSATLVEDSLRLVNDVIPNF 201

Query: 1550 TFGCINDVSGKSIPPQXXXXXGRGPMSLLSQSQSFYSGVFSYCLPSFKSYYFSGSLKLGP 1371
             FGCIN +SG S+PPQ     GRGP+SL++QS S YSG+FSYCLPSFKSYYFSGSLKLGP
Sbjct: 202  AFGCINSISGGSVPPQGLLGLGRGPLSLIAQSGSLYSGLFSYCLPSFKSYYFSGSLKLGP 261

Query: 1370 LGQPKNIRTTPLLKNPHRPSLYYVNLTGINVGRISVPIAPELLAFDPNTGAGTIIDSGTV 1191
             GQPK+IR TPLL+NPHRPSLYYVNLTG++VGR  VPIAPELLAF+PNTGAGTIIDSGTV
Sbjct: 262  AGQPKSIRYTPLLRNPHRPSLYYVNLTGVSVGRTLVPIAPELLAFNPNTGAGTIIDSGTV 321

Query: 1190 ITRFAQPVYNAVRDEFRKQIKGPFSSLGAFDTCFAATNEDIAPAITFRFTGMDLVLPMEN 1011
            ITRF QP+Y A+RDEFRKQ+ GPFSSLGAFDTCFAATNE +APA+T  FTG++LVLPMEN
Sbjct: 322  ITRFVQPIYTAIRDEFRKQVAGPFSSLGAFDTCFAATNEAVAPAVTLHFTGLNLVLPMEN 381

Query: 1010 SLIHSSSGTTACLXXXXXXXXXXXXXXXXXNLQQQNLRIMFDIANSRLGIARELCN 843
            SLIHSS+G+ ACL                 NLQQQNLR++FD+ NSRLGIARELCN
Sbjct: 382  SLIHSSAGSLACLAMAAAPNNVNSVLNVIANLQQQNLRLLFDVPNSRLGIARELCN 437


>ref|XP_002302336.1| predicted protein [Populus trichocarpa] gi|222844062|gb|EEE81609.1|
            predicted protein [Populus trichocarpa]
          Length = 438

 Score =  581 bits (1497), Expect = e-163
 Identities = 291/408 (71%), Positives = 327/408 (80%)
 Frame = -3

Query: 2066 SDISIVHIYGKCSPFHAPKPTSSWANTVIDMASRDQQRLAYLSSLVGQKPTKVPIASGQN 1887
            SD+S++ IY KCSPF  PK   SW NTVI MAS+D +RL YLS+L  QK T VPIA GQ 
Sbjct: 33   SDLSVIPIYSKCSPFVPPKQ-ESWVNTVITMASKDPERLKYLSTLADQKTTAVPIAPGQQ 91

Query: 1886 VLNIGNYVARVSVGTPGQLMFMVLDTSNDAAWIPCSGCTHCTSTMFAPNGSSTYLTMDCS 1707
            VL I NYV RV +GTPGQ MFMVLDTSNDAAW+PCSGCT C+ST F PN S+T  ++DCS
Sbjct: 92   VLKIANYVVRVKLGTPGQQMFMVLDTSNDAAWVPCSGCTGCSSTTFLPNASTTLGSLDCS 151

Query: 1706 MSECAQVRGLACPASGSSTTCQFNQSYGGDSSFSAILSRDSLGLANDVIPNYTFGCINDV 1527
             ++C+QVRG +CPA+GSS  C FNQSYGGDSS +A L +D++ LANDVIP +TFGCIN V
Sbjct: 152  GAQCSQVRGFSCPATGSSA-CLFNQSYGGDSSLTATLVQDAITLANDVIPGFTFGCINAV 210

Query: 1526 SGKSIPPQXXXXXGRGPMSLLSQSQSFYSGVFSYCLPSFKSYYFSGSLKLGPLGQPKNIR 1347
            SG SIPPQ     GRGP+SL+SQ+ + YSGVFSYCLPSFKSYYFSGSLKLGP+GQPK+IR
Sbjct: 211  SGGSIPPQGLLGLGRGPISLISQAGAMYSGVFSYCLPSFKSYYFSGSLKLGPVGQPKSIR 270

Query: 1346 TTPLLKNPHRPSLYYVNLTGINVGRISVPIAPELLAFDPNTGAGTIIDSGTVITRFAQPV 1167
            TTPLL+NPHRPSLYYVNLTG++VGRI VPI  E L FDPNTGAGTIIDSGTVITRF QPV
Sbjct: 271  TTPLLRNPHRPSLYYVNLTGVSVGRIKVPIPSEQLVFDPNTGAGTIIDSGTVITRFVQPV 330

Query: 1166 YNAVRDEFRKQIKGPFSSLGAFDTCFAATNEDIAPAITFRFTGMDLVLPMENSLIHSSSG 987
            Y A+RDEFRKQ+ GP SSLGAFDTCFAATNE  APAIT  F G++LVLPMENSLIHSSSG
Sbjct: 331  YFAIRDEFRKQVNGPISSLGAFDTCFAATNEAEAPAITLHFEGLNLVLPMENSLIHSSSG 390

Query: 986  TTACLXXXXXXXXXXXXXXXXXNLQQQNLRIMFDIANSRLGIARELCN 843
            + ACL                 NLQQQNLRIMFD  NSRLGIARELCN
Sbjct: 391  SLACLSMAAAPNNVNSVLNVIANLQQQNLRIMFDTTNSRLGIARELCN 438


>gb|ABK95290.1| unknown [Populus trichocarpa]
          Length = 438

 Score =  577 bits (1486), Expect = e-162
 Identities = 290/408 (71%), Positives = 326/408 (79%)
 Frame = -3

Query: 2066 SDISIVHIYGKCSPFHAPKPTSSWANTVIDMASRDQQRLAYLSSLVGQKPTKVPIASGQN 1887
            SD+S++ IY KCSPF  PK   SW NTVI MAS+D +RL YLS+L  QK T VPIA GQ 
Sbjct: 33   SDLSVIPIYSKCSPFVPPKQ-ESWVNTVITMASKDPERLKYLSTLADQKTTAVPIAPGQQ 91

Query: 1886 VLNIGNYVARVSVGTPGQLMFMVLDTSNDAAWIPCSGCTHCTSTMFAPNGSSTYLTMDCS 1707
            VL I NYV RV +GTPGQ MFMVLDTSNDAAW+PCSGCT  +ST F PN S+T  ++DCS
Sbjct: 92   VLKIANYVVRVKLGTPGQQMFMVLDTSNDAAWVPCSGCTGFSSTTFLPNASTTLGSLDCS 151

Query: 1706 MSECAQVRGLACPASGSSTTCQFNQSYGGDSSFSAILSRDSLGLANDVIPNYTFGCINDV 1527
             ++C+QVRG +CPA+GSS  C FNQSYGGDSS +A L +D++ LANDVIP +TFGCIN V
Sbjct: 152  GAQCSQVRGFSCPATGSSA-CLFNQSYGGDSSLTATLVQDAITLANDVIPGFTFGCINAV 210

Query: 1526 SGKSIPPQXXXXXGRGPMSLLSQSQSFYSGVFSYCLPSFKSYYFSGSLKLGPLGQPKNIR 1347
            SG SIPPQ     GRGP+SL+SQ+ + YSGVFSYCLPSFKSYYFSGSLKLGP+GQPK+IR
Sbjct: 211  SGGSIPPQGLLGLGRGPISLISQAGAMYSGVFSYCLPSFKSYYFSGSLKLGPVGQPKSIR 270

Query: 1346 TTPLLKNPHRPSLYYVNLTGINVGRISVPIAPELLAFDPNTGAGTIIDSGTVITRFAQPV 1167
            TTPLL+NPHRPSLYYVNLTG++VGRI VPI  E L FDPNTGAGTIIDSGTVITRF QPV
Sbjct: 271  TTPLLRNPHRPSLYYVNLTGVSVGRIKVPIPSEQLVFDPNTGAGTIIDSGTVITRFVQPV 330

Query: 1166 YNAVRDEFRKQIKGPFSSLGAFDTCFAATNEDIAPAITFRFTGMDLVLPMENSLIHSSSG 987
            Y A+RDEFRKQ+ GP SSLGAFDTCFAATNE  APAIT  F G++LVLPMENSLIHSSSG
Sbjct: 331  YFAIRDEFRKQVNGPISSLGAFDTCFAATNEAEAPAITLHFEGLNLVLPMENSLIHSSSG 390

Query: 986  TTACLXXXXXXXXXXXXXXXXXNLQQQNLRIMFDIANSRLGIARELCN 843
            + ACL                 NLQQQNLRIMFD  NSRLGIARELCN
Sbjct: 391  SLACLSMAAAPNNVNSVLNVIANLQQQNLRIMFDTTNSRLGIARELCN 438


>ref|XP_004148418.1| PREDICTED: aspartic proteinase nepenthesin-1-like [Cucumis sativus]
            gi|449518059|ref|XP_004166061.1| PREDICTED: aspartic
            proteinase nepenthesin-1-like [Cucumis sativus]
          Length = 436

 Score =  563 bits (1450), Expect = e-158
 Identities = 286/422 (67%), Positives = 327/422 (77%), Gaps = 1/422 (0%)
 Frame = -3

Query: 2105 AFDHACSSAANYKSDISIVHIYGKCSPFHAPKPTSSWANTVIDMASRDQQRLAYLSSLVG 1926
            AFD  C+S ++   D+S++ IYGKCSPF APK + SW NTVIDMAS+D  R+ YLSSL  
Sbjct: 20   AFD-LCASGSD--GDLSVIPIYGKCSPFTAPK-SESWMNTVIDMASKDPARIRYLSSLTA 75

Query: 1925 QKPTKVPIASGQNVLNIGNYVARVSVGTPGQLMFMVLDTSNDAAWIPCSGCTHCTSTM-F 1749
            QK    PIASGQ VLN+GNYV RV +GTPGQ M+MVLDTSNDAAW PCSGC  C+ST  F
Sbjct: 76   QKTVAAPIASGQQVLNVGNYVVRVQLGTPGQTMYMVLDTSNDAAWAPCSGCIGCSSTTTF 135

Query: 1748 APNGSSTYLTMDCSMSECAQVRGLACPASGSSTTCQFNQSYGGDSSFSAILSRDSLGLAN 1569
            +   SST+ T+DCS  EC Q RGL+CP +G+   C FNQ+YGGDS+FSA L +DSL L  
Sbjct: 136  SAQNSSTFATLDCSKPECTQARGLSCPTTGN-VDCLFNQTYGGDSTFSATLVQDSLHLGP 194

Query: 1568 DVIPNYTFGCINDVSGKSIPPQXXXXXGRGPMSLLSQSQSFYSGVFSYCLPSFKSYYFSG 1389
            +VIPN++FGCI+  SG SIPPQ     GRGP+SL+SQS S YSG+FSYCLPSFKSYYFSG
Sbjct: 195  NVIPNFSFGCISSASGSSIPPQGLMGLGRGPLSLISQSGSLYSGLFSYCLPSFKSYYFSG 254

Query: 1388 SLKLGPLGQPKNIRTTPLLKNPHRPSLYYVNLTGINVGRISVPIAPELLAFDPNTGAGTI 1209
            SLKLGP+GQPK IRTTPLL NPHRPSLYYVNLTGI+VGR+ VPI+PELLAFDPNTGAGTI
Sbjct: 255  SLKLGPVGQPKAIRTTPLLHNPHRPSLYYVNLTGISVGRVLVPISPELLAFDPNTGAGTI 314

Query: 1208 IDSGTVITRFAQPVYNAVRDEFRKQIKGPFSSLGAFDTCFAATNEDIAPAITFRFTGMDL 1029
            IDSGTVITRF   +Y AVRDEFRKQ+ G FS LGAFDTCFA  NE  APAIT   +G+DL
Sbjct: 315  IDSGTVITRFVPAIYTAVRDEFRKQVGGSFSPLGAFDTCFATNNEVSAPAITLHLSGLDL 374

Query: 1028 VLPMENSLIHSSSGTTACLXXXXXXXXXXXXXXXXXNLQQQNLRIMFDIANSRLGIAREL 849
             LPMENSLIHSS+G+ ACL                 NLQQQN RI+FDI NS+LGIAREL
Sbjct: 375  KLPMENSLIHSSAGSLACLAMAAAPNNVNSVVNVIANLQQQNHRILFDINNSKLGIAREL 434

Query: 848  CN 843
            CN
Sbjct: 435  CN 436