BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004123
         (1125 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265080.1| PREDICTED: malate dehydrogenase [NADP], chlo...   428   e-173
ref|NP_001234056.1| malate dehydrogenase [Solanum lycopersicum] ...   422   e-173
ref|XP_002311056.1| predicted protein [Populus trichocarpa] gi|2...   423   e-172
emb|CAN70407.1| hypothetical protein VITISV_021989 [Vitis vinifera]   428   e-171
sp|P21528.2|MDHP_PEA RecName: Full=Malate dehydrogenase [NADP], ...   414   e-170

>ref|XP_002265080.1| PREDICTED: malate dehydrogenase [NADP], chloroplastic [Vitis
            vinifera] gi|296085946|emb|CBI31387.3| unnamed protein
            product [Vitis vinifera]
          Length = 437

 Score =  428 bits (1101), Expect(2) = e-173
 Identities = 215/236 (91%), Positives = 223/236 (94%)
 Frame = -2

Query: 1124 TQNPRIFCSVTSKQVEASVAVKSDDTKRKPECFGVFCLTYDLKAEEETKSWKKMINIAVS 945
            T+N RI CSV S   EA VAV++ D K KPECFGVFC TYDLKAEEETKSWKK+INIAVS
Sbjct: 43   TRNARISCSVASN--EAPVAVQAQDQKIKPECFGVFCTTYDLKAEEETKSWKKLINIAVS 100

Query: 944  GAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSFEALEGVAMELEDSLFPLLREVSI 765
            GAAGMISNHLLFKLA+GEVFGPDQPIA KLLGSERSF+ALEGVAMELEDSLFPLLREVSI
Sbjct: 101  GAAGMISNHLLFKLAAGEVFGPDQPIAFKLLGSERSFQALEGVAMELEDSLFPLLREVSI 160

Query: 764  GIDPYEVFEDVEWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASRNVKVMVVG 585
            GIDPYEVF+DVEWALLIGAKPRGPGMERA LLDINGQIFAEQGKALNAVASRNVKVMVVG
Sbjct: 161  GIDPYEVFQDVEWALLIGAKPRGPGMERADLLDINGQIFAEQGKALNAVASRNVKVMVVG 220

Query: 584  NPCNTNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGN 417
            NPCNTNALIC+KNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGN
Sbjct: 221  NPCNTNALICMKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGN 276



 Score =  209 bits (531), Expect(2) = e-173
 Identities = 99/116 (85%), Positives = 109/116 (93%)
 Frame = -3

Query: 349 STTQVPDFLNAKIDGIPVKEVIRDTKWLEEDFTKMVQTRGGVLIKKWGRSSAASTAVSIV 170
           STTQVPDFLNA+I+G+PVKEVI+D KWLEE+FT+ VQ RGGVLI+KWGRSSAASTAVSIV
Sbjct: 278 STTQVPDFLNARINGLPVKEVIKDIKWLEEEFTEKVQKRGGVLIQKWGRSSAASTAVSIV 337

Query: 169 DAIRSLVTPTPEGDWFSSGVYTAGNPYGIANDIVCSMPCRSKGDGDYELVKDVIFD 2
           DA++SLVTPTPEGDWFSSGVYT GNPYG+A  IV SMPCRSKGDGDYELVKDVIFD
Sbjct: 338 DAMKSLVTPTPEGDWFSSGVYTTGNPYGVAEGIVFSMPCRSKGDGDYELVKDVIFD 393


>ref|NP_001234056.1| malate dehydrogenase [Solanum lycopersicum]
            gi|56562183|emb|CAH60894.1| malate dehydrogenase [Solanum
            lycopersicum]
          Length = 442

 Score =  422 bits (1085), Expect(2) = e-173
 Identities = 205/232 (88%), Positives = 222/232 (95%)
 Frame = -2

Query: 1112 RIFCSVTSKQVEASVAVKSDDTKRKPECFGVFCLTYDLKAEEETKSWKKMINIAVSGAAG 933
            +I CSVTSK+ +A+  V++DD K+K EC+GVFCLTYDLKAEEET SWKKMI ++VSGAAG
Sbjct: 50   QICCSVTSKEAQATTVVQTDDPKKKSECYGVFCLTYDLKAEEETSSWKKMITVSVSGAAG 109

Query: 932  MISNHLLFKLASGEVFGPDQPIALKLLGSERSFEALEGVAMELEDSLFPLLREVSIGIDP 753
            MI+NHLLFKLASGEVFGPDQPIAL+LLGSERS +ALEGVAMELEDSL+PLLREV+IGIDP
Sbjct: 110  MIANHLLFKLASGEVFGPDQPIALRLLGSERSIQALEGVAMELEDSLYPLLREVNIGIDP 169

Query: 752  YEVFEDVEWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASRNVKVMVVGNPCN 573
            YEVF+D EWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASRNVKV+VVGNPCN
Sbjct: 170  YEVFQDAEWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASRNVKVIVVGNPCN 229

Query: 572  TNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGN 417
            TNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGN
Sbjct: 230  TNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGN 281



 Score =  214 bits (545), Expect(2) = e-173
 Identities = 103/116 (88%), Positives = 111/116 (95%)
 Frame = -3

Query: 349 STTQVPDFLNAKIDGIPVKEVIRDTKWLEEDFTKMVQTRGGVLIKKWGRSSAASTAVSIV 170
           STTQVPDFLNAKI+G+PVKEVI+DTKWLEE+FT+ VQ RGGVLI+KWGRSSAASTAVSIV
Sbjct: 283 STTQVPDFLNAKINGLPVKEVIKDTKWLEEEFTEKVQKRGGVLIQKWGRSSAASTAVSIV 342

Query: 169 DAIRSLVTPTPEGDWFSSGVYTAGNPYGIANDIVCSMPCRSKGDGDYELVKDVIFD 2
           DAIRSLVTPTPEGDWFS+GVYT GNPYGIA DIV SMPCRSKGDGDYELVK+VIFD
Sbjct: 343 DAIRSLVTPTPEGDWFSTGVYTNGNPYGIAQDIVFSMPCRSKGDGDYELVKEVIFD 398


>ref|XP_002311056.1| predicted protein [Populus trichocarpa] gi|222850876|gb|EEE88423.1|
            predicted protein [Populus trichocarpa]
          Length = 442

 Score =  423 bits (1088), Expect(2) = e-172
 Identities = 212/235 (90%), Positives = 224/235 (95%)
 Frame = -2

Query: 1121 QNPRIFCSVTSKQVEASVAVKSDDTKRKPECFGVFCLTYDLKAEEETKSWKKMINIAVSG 942
            QN +I CSV   QV A VAV++++ K K ECFGVFC TYDLKAEEETKSWKK+IN+AVSG
Sbjct: 49   QNFKITCSVN--QVPAPVAVQTEEAKSKSECFGVFCQTYDLKAEEETKSWKKLINVAVSG 106

Query: 941  AAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSFEALEGVAMELEDSLFPLLREVSIG 762
            AAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSF+ALEGVAMELEDSL+PLLREVSIG
Sbjct: 107  AAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSFQALEGVAMELEDSLYPLLREVSIG 166

Query: 761  IDPYEVFEDVEWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASRNVKVMVVGN 582
            I+PYEVFEDVEWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASRNVKV+VVGN
Sbjct: 167  INPYEVFEDVEWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASRNVKVIVVGN 226

Query: 581  PCNTNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGN 417
            PCNTNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSN+TIWGN
Sbjct: 227  PCNTNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGN 281



 Score =  209 bits (532), Expect(2) = e-172
 Identities = 100/116 (86%), Positives = 109/116 (93%)
 Frame = -3

Query: 349 STTQVPDFLNAKIDGIPVKEVIRDTKWLEEDFTKMVQTRGGVLIKKWGRSSAASTAVSIV 170
           STTQVPDFLNA+I+GIPVKEVI+D KWLEE+FT+ VQ RGGVLI+KWGRSSAASTAVSI 
Sbjct: 283 STTQVPDFLNARINGIPVKEVIKDHKWLEEEFTEKVQKRGGVLIQKWGRSSAASTAVSIA 342

Query: 169 DAIRSLVTPTPEGDWFSSGVYTAGNPYGIANDIVCSMPCRSKGDGDYELVKDVIFD 2
           DA++SLVTPTPEGDWFSSGVYT+GNPYGIA DIV SMPCRSKGDGDYELVKDV FD
Sbjct: 343 DAMKSLVTPTPEGDWFSSGVYTSGNPYGIAQDIVFSMPCRSKGDGDYELVKDVTFD 398


>emb|CAN70407.1| hypothetical protein VITISV_021989 [Vitis vinifera]
          Length = 434

 Score =  428 bits (1101), Expect(2) = e-171
 Identities = 215/236 (91%), Positives = 223/236 (94%)
 Frame = -2

Query: 1124 TQNPRIFCSVTSKQVEASVAVKSDDTKRKPECFGVFCLTYDLKAEEETKSWKKMINIAVS 945
            T+N RI CSV S   EA VAV++ D K KPECFGVFC TYDLKAEEETKSWKK+INIAVS
Sbjct: 43   TRNARISCSVASN--EAPVAVQAQDQKIKPECFGVFCTTYDLKAEEETKSWKKLINIAVS 100

Query: 944  GAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSFEALEGVAMELEDSLFPLLREVSI 765
            GAAGMISNHLLFKLA+GEVFGPDQPIA KLLGSERSF+ALEGVAMELEDSLFPLLREVSI
Sbjct: 101  GAAGMISNHLLFKLAAGEVFGPDQPIAFKLLGSERSFQALEGVAMELEDSLFPLLREVSI 160

Query: 764  GIDPYEVFEDVEWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASRNVKVMVVG 585
            GIDPYEVF+DVEWALLIGAKPRGPGMERA LLDINGQIFAEQGKALNAVASRNVKVMVVG
Sbjct: 161  GIDPYEVFQDVEWALLIGAKPRGPGMERADLLDINGQIFAEQGKALNAVASRNVKVMVVG 220

Query: 584  NPCNTNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGN 417
            NPCNTNALIC+KNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGN
Sbjct: 221  NPCNTNALICMKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGN 276



 Score =  201 bits (510), Expect(2) = e-171
 Identities = 97/116 (83%), Positives = 107/116 (92%)
 Frame = -3

Query: 349 STTQVPDFLNAKIDGIPVKEVIRDTKWLEEDFTKMVQTRGGVLIKKWGRSSAASTAVSIV 170
           STTQVPDFLNA+I+G+PVKEVI+D KWLEE+FT   + RGGVLI+KWGRSSAASTAVSIV
Sbjct: 278 STTQVPDFLNARINGLPVKEVIKDIKWLEEEFT---EKRGGVLIQKWGRSSAASTAVSIV 334

Query: 169 DAIRSLVTPTPEGDWFSSGVYTAGNPYGIANDIVCSMPCRSKGDGDYELVKDVIFD 2
           DA++SLVTPTPEGDWFSSGVYT GNPYG+A  IV SMPCRSKGDGDYELVKDVIFD
Sbjct: 335 DAMKSLVTPTPEGDWFSSGVYTTGNPYGVAEGIVFSMPCRSKGDGDYELVKDVIFD 390


>sp|P21528.2|MDHP_PEA RecName: Full=Malate dehydrogenase [NADP], chloroplastic; AltName:
            Full=NADP-MDH; Flags: Precursor gi|397475|emb|CAA52614.1|
            malate dehydrogenase (NADP+) [Pisum sativum]
          Length = 441

 Score =  414 bits (1063), Expect(2) = e-170
 Identities = 206/236 (87%), Positives = 218/236 (92%)
 Frame = -2

Query: 1124 TQNPRIFCSVTSKQVEASVAVKSDDTKRKPECFGVFCLTYDLKAEEETKSWKKMINIAVS 945
            TQ+ RI CSV   QV+   A ++ D K KP+C+GVFCLTYDLKAEEETKSWKK+INIAVS
Sbjct: 46   TQHARISCSVAPNQVQVPAA-QTQDPKGKPDCYGVFCLTYDLKAEEETKSWKKLINIAVS 104

Query: 944  GAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSFEALEGVAMELEDSLFPLLREVSI 765
            GAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERS +ALEGVAMELEDSLFPLLREV I
Sbjct: 105  GAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPLLREVVI 164

Query: 764  GIDPYEVFEDVEWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASRNVKVMVVG 585
             IDPYEVF+D EWALLIGAKPRGPG+ERA LLDINGQIFAEQGKALNAVASRN KV+VVG
Sbjct: 165  SIDPYEVFQDAEWALLIGAKPRGPGVERAALLDINGQIFAEQGKALNAVASRNAKVIVVG 224

Query: 584  NPCNTNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGN 417
            NPCNTNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSN+TIWGN
Sbjct: 225  NPCNTNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGN 280



 Score =  212 bits (540), Expect(2) = e-170
 Identities = 101/116 (87%), Positives = 109/116 (93%)
 Frame = -3

Query: 349 STTQVPDFLNAKIDGIPVKEVIRDTKWLEEDFTKMVQTRGGVLIKKWGRSSAASTAVSIV 170
           STTQVPDFLNA+IDG+PVKEVI+D KWLEE+FT+ VQ RGGVLI+KWGRSSAAST+VSIV
Sbjct: 282 STTQVPDFLNARIDGLPVKEVIKDNKWLEEEFTEKVQKRGGVLIQKWGRSSAASTSVSIV 341

Query: 169 DAIRSLVTPTPEGDWFSSGVYTAGNPYGIANDIVCSMPCRSKGDGDYELVKDVIFD 2
           DAIRSL+TPTPEGDWFSSGVYT GNPYGIA DIV SMPCRSKGDGDYELV DVIFD
Sbjct: 342 DAIRSLITPTPEGDWFSSGVYTNGNPYGIAEDIVFSMPCRSKGDGDYELVNDVIFD 397