BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004095
         (1186 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEY75218.1| cytochrome P450 CYP72A129 [Panax ginseng]              335   1e-89
gb|ABC69417.1| CYP72A54 [Nicotiana tabacum]                           308   2e-81
ref|XP_003634774.1| PREDICTED: LOW QUALITY PROTEIN: secologanin ...   298   2e-78
emb|CBI39912.3| unnamed protein product [Vitis vinifera]              297   3e-78
ref|XP_002265676.1| PREDICTED: secologanin synthase-like [Vitis ...   297   3e-78

>gb|AEY75218.1| cytochrome P450 CYP72A129 [Panax ginseng]
          Length = 518

 Score =  335 bits (859), Expect = 1e-89
 Identities = 166/246 (67%), Positives = 197/246 (80%), Gaps = 1/246 (0%)
 Frame = +3

Query: 450  EGTKIFQLQKEQCELSRQVGQSVYIPGMRFLPTKINKRMKEIHNEVTNALRIMINKRLKA 629
            EG KIFQLQ+EQ EL  Q  Q++Y+PGMRFLPTK NKRMKEI  EV  AL+ +INKRLKA
Sbjct: 219  EGRKIFQLQREQAELIIQASQTIYLPGMRFLPTKRNKRMKEIAKEVKIALKSIINKRLKA 278

Query: 630  MEAGECIQHD-LLGILLESNSEVMKANKNAKFGMSVDDVIEECKLFYLAGQETTASLLVW 806
            MEAGE   HD LLGILLESNS+ +K + N  FG++VD+VIEECKLF+ AGQETT++LLVW
Sbjct: 279  MEAGERSSHDDLLGILLESNSKEIKQHGNTNFGLTVDEVIEECKLFFFAGQETTSNLLVW 338

Query: 807  TMILLSQHKDWQERAREEVLQAFGKNKPDIDGLNHLKVLNMILLETLRLYPPGVVLSRAI 986
            TMILLSQH+DWQ+RA+EEVL+ FG NKPD DGLNHLKV+NMILLE LRLYPP + L R I
Sbjct: 339  TMILLSQHQDWQKRAKEEVLRTFGNNKPDFDGLNHLKVVNMILLEVLRLYPPILSLDRTI 398

Query: 987  FQDMKLGDMSLPAGXXXXXXXXXXXXDRDIWGDDVMEFKPERFSEGVFKATKGKSVYFPF 1166
            ++++KLG++SLPAG            D++IWGDD  EF PERFSEGV KATKG+  YFPF
Sbjct: 399  YEEIKLGEISLPAGVILLLPIILLHYDQEIWGDDAKEFNPERFSEGVLKATKGRVTYFPF 458

Query: 1167 SMGPRV 1184
            S GPR+
Sbjct: 459  SWGPRI 464



 Score =  140 bits (352), Expect = 8e-31
 Identities = 62/92 (67%), Positives = 78/92 (84%)
 Frame = +3

Query: 12  HGKKSFLWLGTRPLVYITDPDHVKEVLCKFYEFKKPRGGNPLSALLVTGLIDADGDRWVK 191
           +GK SFLWLG +P+V+I +PDH+K+VL KFY+F+KPR  NPL+ LL TG+ DA+GDRW K
Sbjct: 92  YGKNSFLWLGPKPMVHIMNPDHIKDVLSKFYQFQKPRH-NPLTKLLATGVADAEGDRWAK 150

Query: 192 HRKIINPAFHLEKLKNMLPAFYLSCNDIMKTW 287
           HRK+INPAFHLEKLKNMLPA YLS ++I+  W
Sbjct: 151 HRKLINPAFHLEKLKNMLPAIYLSSSEIVTKW 182


>gb|ABC69417.1| CYP72A54 [Nicotiana tabacum]
          Length = 517

 Score =  308 bits (788), Expect = 2e-81
 Identities = 151/245 (61%), Positives = 184/245 (75%)
 Frame = +3

Query: 450  EGTKIFQLQKEQCELSRQVGQSVYIPGMRFLPTKINKRMKEIHNEVTNALRIMINKRLKA 629
            +G +IF+LQKEQ EL  Q  + +YIPG RF+PTK NKRMK+I  EV + +  +INKR++ 
Sbjct: 220  DGRQIFELQKEQAELILQAARWLYIPGWRFVPTKRNKRMKQIAKEVRSLVLGIINKRIRE 279

Query: 630  MEAGECIQHDLLGILLESNSEVMKANKNAKFGMSVDDVIEECKLFYLAGQETTASLLVWT 809
            M+AGE  + DLLGILLESN + ++ + N  FGM++D+VIEECKLFY AGQETT+ LLVWT
Sbjct: 280  MKAGEAAKDDLLGILLESNFKEIQMHGNKNFGMTIDEVIEECKLFYFAGQETTSVLLVWT 339

Query: 810  MILLSQHKDWQERAREEVLQAFGKNKPDIDGLNHLKVLNMILLETLRLYPPGVVLSRAIF 989
            +ILLS+H DWQERAREEV Q FG NKPD D LN LKV+ MI  E LRLYPPG+ +SR + 
Sbjct: 340  LILLSKHVDWQERAREEVHQVFGSNKPDYDALNQLKVVTMIFNEVLRLYPPGITISRTVH 399

Query: 990  QDMKLGDMSLPAGXXXXXXXXXXXXDRDIWGDDVMEFKPERFSEGVFKATKGKSVYFPFS 1169
            +D KLG++SLPAG            D +IWGDD  EFKPERFSEGV KATKGK  YFPFS
Sbjct: 400  EDTKLGNLSLPAGIQLVLPAIWLHHDNEIWGDDAKEFKPERFSEGVNKATKGKFAYFPFS 459

Query: 1170 MGPRV 1184
             GPR+
Sbjct: 460  WGPRI 464



 Score =  119 bits (297), Expect = 2e-24
 Identities = 49/93 (52%), Positives = 75/93 (80%)
 Frame = +3

Query: 9   KHGKKSFLWLGTRPLVYITDPDHVKEVLCKFYEFKKPRGGNPLSALLVTGLIDADGDRWV 188
           ++GK SF+WLG +P ++ITDP+ ++E+L K Y +++ +G NP++ LL  GL+  + ++W 
Sbjct: 93  QYGKNSFMWLGPKPTMFITDPELIREILSKSYIYQEIQG-NPITKLLAQGLVSYEAEKWA 151

Query: 189 KHRKIINPAFHLEKLKNMLPAFYLSCNDIMKTW 287
           KHRKIINPAFHL+KLK+MLP+FYLSC D+++ W
Sbjct: 152 KHRKIINPAFHLDKLKHMLPSFYLSCCDMLRKW 184


>ref|XP_003634774.1| PREDICTED: LOW QUALITY PROTEIN: secologanin synthase-like [Vitis
            vinifera]
          Length = 516

 Score =  298 bits (763), Expect = 2e-78
 Identities = 153/246 (62%), Positives = 181/246 (73%), Gaps = 1/246 (0%)
 Frame = +3

Query: 450  EGTKIFQLQKEQCELSRQVGQSVYIPGMRFLPTKINKRMKEIHNEVTNALRIMINKRLKA 629
            EG +IFQLQKEQ  L+ QV  SVYIPG RFLPTK N+RMK+I  EV   LR ++NKR KA
Sbjct: 217  EGRRIFQLQKEQTHLAIQVTMSVYIPGWRFLPTKTNRRMKQISKEVYALLRGIVNKREKA 276

Query: 630  MEAGECIQHDLLGILLESN-SEVMKANKNAKFGMSVDDVIEECKLFYLAGQETTASLLVW 806
            M+AGE    DLLGIL+ESN  E+ +   N K GMSV DVIEECKLFYLAGQETT+ LLVW
Sbjct: 277  MKAGETANSDLLGILMESNFREIQEHQNNKKIGMSVRDVIEECKLFYLAGQETTSVLLVW 336

Query: 807  TMILLSQHKDWQERAREEVLQAFGKNKPDIDGLNHLKVLNMILLETLRLYPPGVVLSRAI 986
            TM+LLS+H +WQ RAREEVLQ FG  KP+ DGLNHLK++ MI  E LRLYPP  +L+RA+
Sbjct: 337  TMVLLSEHPNWQARAREEVLQVFGNKKPEADGLNHLKIVTMIFHEVLRLYPPIAMLARAV 396

Query: 987  FQDMKLGDMSLPAGXXXXXXXXXXXXDRDIWGDDVMEFKPERFSEGVFKATKGKSVYFPF 1166
            ++D ++GDM  PAG            D +IWGDD  EF PERF+EGV KATK +  +FPF
Sbjct: 397  YKDTQVGDMCFPAG-VQVXPTILVHHDHEIWGDDAKEFNPERFAEGVLKATKNQVSFFPF 455

Query: 1167 SMGPRV 1184
              GPRV
Sbjct: 456  GWGPRV 461



 Score =  110 bits (274), Expect = 9e-22
 Identities = 50/93 (53%), Positives = 68/93 (73%)
 Frame = +3

Query: 9   KHGKKSFLWLGTRPLVYITDPDHVKEVLCKFYEFKKPRGGNPLSALLVTGLIDADGDRWV 188
           K+GK  F W G  P+V I +P+ ++++L K   F+KP   +PL  LLV+GL+  +G+RW 
Sbjct: 90  KYGKNFFAWFGPNPMVNIMEPELIRDILLKSNVFQKPPP-HPLGKLLVSGLVTLEGERWA 148

Query: 189 KHRKIINPAFHLEKLKNMLPAFYLSCNDIMKTW 287
           K RK INPAFHLEKLKNMLPAF+LSC+D++  W
Sbjct: 149 KRRKNINPAFHLEKLKNMLPAFHLSCSDMVTKW 181


>emb|CBI39912.3| unnamed protein product [Vitis vinifera]
          Length = 440

 Score =  297 bits (761), Expect = 3e-78
 Identities = 151/246 (61%), Positives = 178/246 (72%), Gaps = 1/246 (0%)
 Frame = +3

Query: 450  EGTKIFQLQKEQCELSRQVGQSVYIPGMRFLPTKINKRMKEIHNEVTNALRIMINKRLKA 629
            EG +IFQLQKEQ  L+ QV  SVYIPG RFLPTK N+RMK+I  EV   LR +INKR KA
Sbjct: 140  EGRRIFQLQKEQTHLAIQVTMSVYIPGWRFLPTKTNRRMKQISKEVYALLRGIINKREKA 199

Query: 630  MEAGECIQHDLLGILLESN-SEVMKANKNAKFGMSVDDVIEECKLFYLAGQETTASLLVW 806
            M+AGE    DLLGIL+ESN  E+ +   N K  MSV DVIEECKLFYLAGQETT+ LLVW
Sbjct: 200  MKAGETANSDLLGILMESNFREIQEHQNNKKIRMSVKDVIEECKLFYLAGQETTSVLLVW 259

Query: 807  TMILLSQHKDWQERAREEVLQAFGKNKPDIDGLNHLKVLNMILLETLRLYPPGVVLSRAI 986
            TM+LLS+H +WQ RAREEVLQ FG  KP+  GLNHLK++ MI  E LRLYPP  +L+RA+
Sbjct: 260  TMVLLSEHPNWQARAREEVLQVFGNKKPEAAGLNHLKIVTMIFHEVLRLYPPVAMLARAV 319

Query: 987  FQDMKLGDMSLPAGXXXXXXXXXXXXDRDIWGDDVMEFKPERFSEGVFKATKGKSVYFPF 1166
            ++D ++GDM  PAG            D +IWGDD  EF PERF+EGV KATK +  +FPF
Sbjct: 320  YKDTQVGDMCFPAGVQVVLPTILVHHDHEIWGDDAKEFNPERFAEGVLKATKNQVSFFPF 379

Query: 1167 SMGPRV 1184
              GPRV
Sbjct: 380  GWGPRV 385



 Score = 94.0 bits (232), Expect = 7e-17
 Identities = 43/76 (56%), Positives = 59/76 (77%)
 Frame = +3

Query: 60  ITDPDHVKEVLCKFYEFKKPRGGNPLSALLVTGLIDADGDRWVKHRKIINPAFHLEKLKN 239
           + +P+ +++VL K   F+KP   +PL  LLV+GL+  +G+RW K RKIINPAFHLEKLKN
Sbjct: 30  MVEPELIRDVLLKSNVFQKPPP-HPLGKLLVSGLVTLEGERWAKRRKIINPAFHLEKLKN 88

Query: 240 MLPAFYLSCNDIMKTW 287
           MLPAF+LSC+D++  W
Sbjct: 89  MLPAFHLSCSDMVTKW 104


>ref|XP_002265676.1| PREDICTED: secologanin synthase-like [Vitis vinifera]
          Length = 516

 Score =  297 bits (761), Expect = 3e-78
 Identities = 151/246 (61%), Positives = 178/246 (72%), Gaps = 1/246 (0%)
 Frame = +3

Query: 450  EGTKIFQLQKEQCELSRQVGQSVYIPGMRFLPTKINKRMKEIHNEVTNALRIMINKRLKA 629
            EG +IFQLQKEQ  L+ QV  SVYIPG RFLPTK N+RMK+I  EV   LR +INKR KA
Sbjct: 216  EGRRIFQLQKEQTHLAIQVTMSVYIPGWRFLPTKTNRRMKQISKEVYALLRGIINKREKA 275

Query: 630  MEAGECIQHDLLGILLESN-SEVMKANKNAKFGMSVDDVIEECKLFYLAGQETTASLLVW 806
            M+AGE    DLLGIL+ESN  E+ +   N K  MSV DVIEECKLFYLAGQETT+ LLVW
Sbjct: 276  MKAGETANSDLLGILMESNFREIQEHQNNKKIRMSVKDVIEECKLFYLAGQETTSVLLVW 335

Query: 807  TMILLSQHKDWQERAREEVLQAFGKNKPDIDGLNHLKVLNMILLETLRLYPPGVVLSRAI 986
            TM+LLS+H +WQ RAREEVLQ FG  KP+  GLNHLK++ MI  E LRLYPP  +L+RA+
Sbjct: 336  TMVLLSEHPNWQARAREEVLQVFGNKKPEAAGLNHLKIVTMIFHEVLRLYPPVAMLARAV 395

Query: 987  FQDMKLGDMSLPAGXXXXXXXXXXXXDRDIWGDDVMEFKPERFSEGVFKATKGKSVYFPF 1166
            ++D ++GDM  PAG            D +IWGDD  EF PERF+EGV KATK +  +FPF
Sbjct: 396  YKDTQVGDMCFPAGVQVVLPTILVHHDHEIWGDDAKEFNPERFAEGVLKATKNQVSFFPF 455

Query: 1167 SMGPRV 1184
              GPRV
Sbjct: 456  GWGPRV 461



 Score =  105 bits (261), Expect = 3e-20
 Identities = 48/85 (56%), Positives = 64/85 (75%)
 Frame = +3

Query: 33  WLGTRPLVYITDPDHVKEVLCKFYEFKKPRGGNPLSALLVTGLIDADGDRWVKHRKIINP 212
           W G  P+V I +P+ +++VL K   F+KP   +PL  LLV+GL+  +G+RW K RKIINP
Sbjct: 97  WFGPNPMVNIMEPELIRDVLLKSNVFQKPPP-HPLGKLLVSGLVTLEGERWAKRRKIINP 155

Query: 213 AFHLEKLKNMLPAFYLSCNDIMKTW 287
           AFHLEKLKNMLPAF+LSC+D++  W
Sbjct: 156 AFHLEKLKNMLPAFHLSCSDMVTKW 180