BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004073
         (3451 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002532897.1| ubiquitin-protein ligase, putative [Ricinus ...  1357   0.0  
ref|XP_004136686.1| PREDICTED: probable ubiquitin conjugation fa...  1333   0.0  
ref|XP_003543890.1| PREDICTED: probable ubiquitin conjugation fa...  1333   0.0  
ref|XP_003554717.1| PREDICTED: probable ubiquitin conjugation fa...  1323   0.0  
ref|XP_002324089.1| predicted protein [Populus trichocarpa] gi|2...  1316   0.0  

>ref|XP_002532897.1| ubiquitin-protein ligase, putative [Ricinus communis]
            gi|223527331|gb|EEF29477.1| ubiquitin-protein ligase,
            putative [Ricinus communis]
          Length = 1031

 Score = 1357 bits (3513), Expect = 0.0
 Identities = 679/848 (80%), Positives = 744/848 (87%), Gaps = 9/848 (1%)
 Frame = -3

Query: 2777 DFDSVDPILKQLYEDLRGSVLTVSALGNFQQPLRALLYLVNTPAGAKSLVSHHWWIPKGA 2598
            DFDS+DPILK LYEDLRG+V+ VSA+GNFQQPL ALL+L+  P G KSLV+H WWIPKGA
Sbjct: 184  DFDSLDPILKGLYEDLRGNVIKVSAMGNFQQPLGALLHLITYPVGVKSLVNHPWWIPKGA 243

Query: 2597 YLNGRVIEMTSILGPFFHVSALPDQTIFRSQPDVGQQCFAEASTRRPADLLSSFTTIRTV 2418
            YLNGRVIEMTSILGPFFHVSALPD TIF+S+PDVGQQCF+E STRRP+DLLSSF TI+T 
Sbjct: 244  YLNGRVIEMTSILGPFFHVSALPDHTIFKSEPDVGQQCFSEVSTRRPSDLLSSFATIKTF 303

Query: 2417 MNNLYDGLAEVLKCLLKNTNTRENVLEYLAEVINKNKSRSHIQVDPISCASSGMFVNLSA 2238
            MNNLYDGL +VL+ LLKN +TRENVL+YLAEVIN+N SR+HIQVDP+SCASSGMFVNLSA
Sbjct: 304  MNNLYDGLEQVLRILLKNGDTRENVLQYLAEVINRNSSRAHIQVDPLSCASSGMFVNLSA 363

Query: 2237 VMLRLCEPFLDANLTKRDKIDSKYVFNSSRLDLRELTALHATSEEVAEWFTKNNPGKVDI 2058
            VMLRLC PFLD NLTKRDKIDS+YVF S+RLDLR LTALHA+SEEV EW  K N GK ++
Sbjct: 364  VMLRLCNPFLDPNLTKRDKIDSQYVFESNRLDLRGLTALHASSEEVTEWMNKGNHGKTEV 423

Query: 2057 NSTSNDGENRLLXXXXXXXXXXXXXA---------KYPFICECFFMTARVLNLGLLKGFS 1905
            +  S+DGENRLL                       KY FICECFFMTARVLNLGLLK FS
Sbjct: 424  SVQSSDGENRLLQSQEATSSGSGTNKPTSSSGQKAKYTFICECFFMTARVLNLGLLKAFS 483

Query: 1904 DFKHLVQDIQRSEDSLTSLKAMQEQAPSPQLNQDITRLEKELELYSQEKLCYEAQILRDG 1725
            DFKHLVQDI R ED+L++LKAMQEQ+P+PQ+  DI RLEK+LELYSQEK CYEAQILRD 
Sbjct: 484  DFKHLVQDISRCEDTLSTLKAMQEQSPTPQVQVDIARLEKDLELYSQEKFCYEAQILRDE 543

Query: 1724 GFLQQALSFYRLMVVWLARLVGGFKMPLPPTCPREFASMPEHFVEDVMEFLIFASRIPKA 1545
              +Q ALSFYRLMVVWL  LVGGF+MPLPPTCP EFAS+PEHFVED ME LIFASRIPKA
Sbjct: 544  TLIQSALSFYRLMVVWLVDLVGGFRMPLPPTCPMEFASLPEHFVEDAMELLIFASRIPKA 603

Query: 1544 LDGAMLDDFMNFIIMFMGSPEYIKNPYLRAKMVEVLNCWMPRRSGSSATSTLFEGHQLSL 1365
            LDG +LDDFMNFIIMFM SP YI+NPYLRAKMVEVLNCWMPRRSGSS T+TLFEGH LSL
Sbjct: 604  LDGVVLDDFMNFIIMFMASPTYIRNPYLRAKMVEVLNCWMPRRSGSSDTATLFEGHHLSL 663

Query: 1364 EYLVKNLLKVYVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKG 1185
            EYLV+NLLK+YVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIA+EEEKG
Sbjct: 664  EYLVRNLLKLYVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAREEEKG 723

Query: 1184 VYLNFLNFLVNDSIYLLDESLNKILELKELEAEMSNTVEWERRPSQERQERTRLFHSQEN 1005
            VYLNFLNFL+NDSIYLLDESLNKILELKELEAEMSNT EWE+RP+QERQERTRLFHSQEN
Sbjct: 724  VYLNFLNFLINDSIYLLDESLNKILELKELEAEMSNTAEWEQRPAQERQERTRLFHSQEN 783

Query: 1004 IIRIDMKLANEDVTMLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVGPQRKSLSLKDP 825
            IIRIDMKLANEDV+MLAFTSEQITAPFLL EMVERVASMLNYFLLQLVGPQRKSLSLKDP
Sbjct: 784  IIRIDMKLANEDVSMLAFTSEQITAPFLLLEMVERVASMLNYFLLQLVGPQRKSLSLKDP 843

Query: 824  EKYEFRPKHLLKQIVNIYVHLARGDKHNIFPSAITKDGRSYNEQLFSAAASVLQRIGEDV 645
            EKYEFRPK LLKQIV+IYVHL+RGD  NIFP+AI+KDGRSYNEQLFSAAA VL+RIGED+
Sbjct: 844  EKYEFRPKQLLKQIVHIYVHLSRGDAENIFPAAISKDGRSYNEQLFSAAADVLRRIGEDM 903

Query: 644  RVIQEFIELGNXXXXXXXXXXXXXXALGEIPDEFLDPIQYTLMEDPVILPSSRITVDRPV 465
            RVIQEF+ELG+               LGEIPDEFLDPIQYTLM+DPVILPSSRIT+DRPV
Sbjct: 904  RVIQEFVELGSKAKVAASEAMDTEAVLGEIPDEFLDPIQYTLMKDPVILPSSRITIDRPV 963

Query: 464  IQRHLLSDSTDPFNRSHLTADMLIPDTELKAQINEFLRSRELNRHGDNLSMQSTKDTIQT 285
            IQRHLLSD+TDPFNRSHLTADMLIP+ ELKA+I EF+R++EL R G++ SMQS+K TIQT
Sbjct: 964  IQRHLLSDATDPFNRSHLTADMLIPNVELKARIEEFIRNQELKRRGEDFSMQSSKATIQT 1023

Query: 284  TDEMPLID 261
            T    LID
Sbjct: 1024 TTGEMLID 1031



 Score =  177 bits (448), Expect = 2e-41
 Identities = 99/160 (61%), Positives = 116/160 (72%), Gaps = 2/160 (1%)
 Frame = -1

Query: 3412 ATTKPQRSPAEIEDIILRKIFLVSLQDTM--ENDSRIVYLEMTAAEILSEGGDLRLSRDL 3239
            ++ KPQRS  EIEDIILRKI LVSL D      DSRIVYLEM AAEILSEG DL+LSRDL
Sbjct: 3    SSAKPQRSLQEIEDIILRKILLVSLTDDSISTTDSRIVYLEMAAAEILSEGKDLKLSRDL 62

Query: 3238 MERVLIDRLSGNFITAEPPFIYLLGCYRRAYDEGKKVLSMKDKSLSADMVEVTKQAKKLA 3059
            +ERVLIDRLSG F  +EPPF YLLGCYRRA +E +K+ +MKDK++  ++    KQAK+L 
Sbjct: 63   IERVLIDRLSGQFPRSEPPFQYLLGCYRRATEEERKISNMKDKNVKLELELSIKQAKRLF 122

Query: 3058 VSYCRIHLGNPDMFPXXXXXXXXXXXXXXSQLLPLIFSEV 2939
            +SYCRIHLGNPDMF               S LLPLIF+ +
Sbjct: 123  ISYCRIHLGNPDMF---GGGDFDSKKSTLSPLLPLIFASL 159


>ref|XP_004136686.1| PREDICTED: probable ubiquitin conjugation factor E4-like [Cucumis
            sativus] gi|449494681|ref|XP_004159617.1| PREDICTED:
            probable ubiquitin conjugation factor E4-like [Cucumis
            sativus]
          Length = 1043

 Score = 1333 bits (3451), Expect = 0.0
 Identities = 680/855 (79%), Positives = 734/855 (85%), Gaps = 16/855 (1%)
 Frame = -3

Query: 2777 DFDSVDPILKQLYEDLRGSVLTVSALGNFQQPLRALLYLVNTPAGAKSLVSHHWWIPKGA 2598
            DFD+++PILK LYEDLRGSVL VSALGNFQQPLRAL +LV+ P GAKSLV+H WWIP G 
Sbjct: 189  DFDTLEPILKGLYEDLRGSVLKVSALGNFQQPLRALRFLVSFPVGAKSLVNHPWWIPTGK 248

Query: 2597 YLNGRVIEMTSILGPFFHVSALPDQTIFRSQPDVGQQCFAEASTRRPADLLSSFTTIRTV 2418
            Y NGRVIEMTSILGPFFHVSALPD  IF+SQPDVGQQCF+EASTRRPADLLSSFTTI+TV
Sbjct: 249  YSNGRVIEMTSILGPFFHVSALPDHAIFKSQPDVGQQCFSEASTRRPADLLSSFTTIKTV 308

Query: 2417 MNNLYDGLAEVLKCLLKNTNTRENVLEYLAEVINKNKSRSHIQVDPISCASSGMFVNLSA 2238
            MNNLYDGL+EVL  LLKNT TRENVLEYLAEVIN+N SR+HIQVDP+SCASSGMFVNLSA
Sbjct: 309  MNNLYDGLSEVLLSLLKNTETRENVLEYLAEVINRNSSRAHIQVDPLSCASSGMFVNLSA 368

Query: 2237 VMLRLCEPFLDANLTKRDKIDSKYVFNSSRLDLRELTALHATSEEVAEWFTKNNPGKVDI 2058
            +MLRLCEPFLDANLTKRDKID KYV  S+RL+LR LTALHA+SEEV EW       + D 
Sbjct: 369  IMLRLCEPFLDANLTKRDKIDPKYVCYSNRLELRGLTALHASSEEVTEWINNGTQLRTDN 428

Query: 2057 NSTSNDGENRLLXXXXXXXXXXXXXA--------------KYPFICECFFMTARVLNLGL 1920
               S+D E+RLL                            +YPFICECFFMTARVLNLGL
Sbjct: 429  PGQSSDSESRLLQSQEASSSGSNATIGSSTAKARSSSDKTRYPFICECFFMTARVLNLGL 488

Query: 1919 LKGFSDFKHLVQDIQRSEDSLTSLKAMQEQAPSPQLNQDITRLEKELELYSQEKLCYEAQ 1740
            LK FSDFKHLVQDI R ED+L++LKAMQ Q P+PQL  DI RLEKE+ELYSQEKLCYEAQ
Sbjct: 489  LKAFSDFKHLVQDISRCEDTLSTLKAMQGQGPAPQLEMDIARLEKEIELYSQEKLCYEAQ 548

Query: 1739 ILRDGGFLQQALSFYRLMVVWLARLVGGFKMPLPPTCPREFASMPEHFVEDVMEFLIFAS 1560
            ILRDG  +QQAL+FYRLMV+WL  LVGGFKMPLP  CP EFASMPEHFVED ME LIFAS
Sbjct: 549  ILRDGTLIQQALTFYRLMVIWLVGLVGGFKMPLPSACPMEFASMPEHFVEDAMELLIFAS 608

Query: 1559 RIPKALDGAMLDDFMNFIIMFMGSPEYIKNPYLRAKMVEVLNCWMPRRSGSSATSTLFEG 1380
            RIPKALDG  LDDFMNFIIMFM SPEYI+NPYLRAKMVEVLNCW+PRRSGSS T+TLFEG
Sbjct: 609  RIPKALDGINLDDFMNFIIMFMASPEYIRNPYLRAKMVEVLNCWIPRRSGSSVTATLFEG 668

Query: 1379 HQLSLEYLVKNLLKVYVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAK 1200
            HQLSLEYLV+NLLK+YVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWR IAK
Sbjct: 669  HQLSLEYLVRNLLKLYVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRMIAK 728

Query: 1199 EEEKGVYLNFLNFLVNDSIYLLDESLNKILELKELEAEMSNTVEWERRPSQERQERTRLF 1020
            EEEKGVYLNFLNFL+NDSIYLLDESLNKILELKELEAEMSNT EWERRP+QERQERTRLF
Sbjct: 729  EEEKGVYLNFLNFLINDSIYLLDESLNKILELKELEAEMSNTAEWERRPAQERQERTRLF 788

Query: 1019 HSQENIIRIDMKLANEDVTMLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVGPQRKSL 840
            HSQENIIRIDMKLANEDV+MLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVGPQRKSL
Sbjct: 789  HSQENIIRIDMKLANEDVSMLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVGPQRKSL 848

Query: 839  SLKDPEKYEFRPKHLLKQIVNIYVHLARGDKHNIFPSAITKDGRSYNEQLFSAAASVL-Q 663
            SLKDPEKYEFRP+ LLKQIV IYVHLARGD  NIFP+AI+KDGRSYNEQLF+AAA VL +
Sbjct: 849  SLKDPEKYEFRPRELLKQIVQIYVHLARGDTENIFPAAISKDGRSYNEQLFTAAADVLIR 908

Query: 662  RIGEDVRVIQEFIELGNXXXXXXXXXXXXXXALGEIPDEFLDPIQYTLMEDPVILPSSRI 483
            RI ED R+IQEF +LGN               LG+IPDEFLDPIQYTLM+DPVILPSSRI
Sbjct: 909  RIREDSRIIQEFTDLGNKAKDAASEAMDAEATLGDIPDEFLDPIQYTLMKDPVILPSSRI 968

Query: 482  TVDRPVIQRHLLSDSTDPFNRSHLTADMLIPDTELKAQINEFLRSRELNRHGD-NLSMQS 306
            TVDRPVIQRHLLSDSTDPFNRSHLTADMLIP+ ELKA+I EF+RS+EL +  D  ++MQS
Sbjct: 969  TVDRPVIQRHLLSDSTDPFNRSHLTADMLIPNEELKARIKEFIRSQELKKQLDGGVAMQS 1028

Query: 305  TKDTIQTTDEMPLID 261
            +K TIQ T    LID
Sbjct: 1029 SKATIQPTSGEMLID 1043



 Score =  211 bits (538), Expect = 8e-52
 Identities = 110/171 (64%), Positives = 134/171 (78%), Gaps = 1/171 (0%)
 Frame = -1

Query: 3415 MATTKPQRSPAEIEDIILRKIFLVSLQDTMENDSRIVYLEMTAAEILSEGGDLRLSRDLM 3236
            MAT+KPQRSP E+EDIILRK+FL+SL DT ++DSRIVYLE TAAE+LSEG  LR+SRD+M
Sbjct: 1    MATSKPQRSPEEVEDIILRKVFLISLTDTSDSDSRIVYLEQTAAELLSEGKPLRISRDVM 60

Query: 3235 ERVLIDRLSGNFITAEPPFIYLLGCYRRAYDEGKKVLSMKDKSLSADMVEVTKQAKKLAV 3056
            ER++IDRLS +  +AEPPF YL+GCYRRA+DE KK+ SMKDK+L +DM    KQAKKL +
Sbjct: 61   ERIIIDRLSAHVPSAEPPFQYLIGCYRRAHDETKKIASMKDKTLRSDMEIALKQAKKLTI 120

Query: 3055 SYCRIHLGNPDMFPXXXXXXXXXXXXXXSQLLPLIFSEV-SSTVDGFGGSS 2906
            SYCRIHLGNP++F               S LLPLIFSEV  S++DGFG S+
Sbjct: 121  SYCRIHLGNPELF---SSGADLGTNSNTSPLLPLIFSEVGGSSMDGFGAST 168


>ref|XP_003543890.1| PREDICTED: probable ubiquitin conjugation factor E4-like [Glycine
            max]
          Length = 1038

 Score = 1333 bits (3450), Expect = 0.0
 Identities = 677/852 (79%), Positives = 734/852 (86%), Gaps = 13/852 (1%)
 Frame = -3

Query: 2777 DFDSVDPILKQLYEDLRGSVLTVSALGNFQQPLRALLYLVNTPAGAKSLVSHHWWIPKGA 2598
            DFDS+D ILK LYE+LRGSV+ VSALGNFQ  LRALLYLV  P GAKSLV+H WWIPKG 
Sbjct: 190  DFDSLDKILKGLYEELRGSVMKVSALGNFQDSLRALLYLVRFPIGAKSLVNHEWWIPKGV 249

Query: 2597 YLNGRVIEMTSILGPFFHVSALPDQTIFRSQPDVGQQCFAEASTRRPADLLSSFTTIRTV 2418
            Y+NGR IEMTSILGPFFH+SALPDQ  F+ QPDVGQQCF++ASTRRPADLLSSF+TI+TV
Sbjct: 250  YVNGRAIEMTSILGPFFHISALPDQAFFKGQPDVGQQCFSDASTRRPADLLSSFSTIKTV 309

Query: 2417 MNNLYDGLAEVLKCLLKNTNTRENVLEYLAEVINKNKSRSHIQVDPISCASSGMFVNLSA 2238
            MNNLYDGLAEVL  LLK+ +TRENVL+YLAEVIN N SR+HIQVDPI+CASSGMFVNLSA
Sbjct: 310  MNNLYDGLAEVLLILLKSQDTRENVLKYLAEVININASRAHIQVDPITCASSGMFVNLSA 369

Query: 2237 VMLRLCEPFLDANLTKRDKIDSKYVFNSSRLDLRELTALHATSEEVAEWFTKNNPGKVDI 2058
            V+LRLCEPFLDANLTKRDKID+KYV  S+RL L  LTALHA+SEEV EW    NP K   
Sbjct: 370  VVLRLCEPFLDANLTKRDKIDAKYVHYSNRLKLSGLTALHASSEEVIEWLNSKNPAKTGA 429

Query: 2057 NSTSNDGENRL------------LXXXXXXXXXXXXXAKYPFICECFFMTARVLNLGLLK 1914
             +  ND + RL                           KY FICECFFMTARVLNLGLLK
Sbjct: 430  TNQYNDDQKRLQQSQEASSSGSNADELSNENSARAEKTKYSFICECFFMTARVLNLGLLK 489

Query: 1913 GFSDFKHLVQDIQRSEDSLTSLKAMQEQAPSPQLNQDITRLEKELELYSQEKLCYEAQIL 1734
             FSDFKHLVQDI R ED+L++LKAMQE+ P+PQ   DI RLEKE+ELYSQEKLCYEAQIL
Sbjct: 490  AFSDFKHLVQDISRCEDALSTLKAMQERTPTPQAELDINRLEKEMELYSQEKLCYEAQIL 549

Query: 1733 RDGGFLQQALSFYRLMVVWLARLVGGFKMPLPPTCPREFASMPEHFVEDVMEFLIFASRI 1554
            RD   +Q ALSFYRLM+VWL  LVGG KMPLPPTCP EF++MPEHFVED ME LIFASRI
Sbjct: 550  RDNTLIQNALSFYRLMIVWLVGLVGGLKMPLPPTCPMEFSTMPEHFVEDAMELLIFASRI 609

Query: 1553 PKALDGAMLDDFMNFIIMFMGSPEYIKNPYLRAKMVEVLNCWMPRRSGSSATSTLFEGHQ 1374
            PKALDG +LD+FMNFIIMFM SPE+IKNPYLRAKMVEVLNCWMPRRSGS+AT+TLFEGHQ
Sbjct: 610  PKALDGVVLDEFMNFIIMFMASPEFIKNPYLRAKMVEVLNCWMPRRSGSTATATLFEGHQ 669

Query: 1373 LSLEYLVKNLLKVYVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEE 1194
            LSLEYLV+NLLK+YVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEE
Sbjct: 670  LSLEYLVRNLLKLYVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEE 729

Query: 1193 EKGVYLNFLNFLVNDSIYLLDESLNKILELKELEAEMSNTVEWERRPSQERQERTRLFHS 1014
            EKGVYLNFLNFL+NDSIYLLDESLNKILELKELEAEMSNTVEWERRP QERQERTRLFHS
Sbjct: 730  EKGVYLNFLNFLINDSIYLLDESLNKILELKELEAEMSNTVEWERRPVQERQERTRLFHS 789

Query: 1013 QENIIRIDMKLANEDVTMLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVGPQRKSLSL 834
            QENIIRIDMKLANEDV+MLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVGPQRKSLSL
Sbjct: 790  QENIIRIDMKLANEDVSMLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVGPQRKSLSL 849

Query: 833  KDPEKYEFRPKHLLKQIVNIYVHLARGDKHNIFPSAITKDGRSYNEQLFSAAASVLQRIG 654
            KDPEKYEFRPKHLLKQIV+IYVHLARGD ++IFP+AI+KDGRSYN+QLFSA A VL RIG
Sbjct: 850  KDPEKYEFRPKHLLKQIVHIYVHLARGDTNSIFPAAISKDGRSYNDQLFSAGADVLHRIG 909

Query: 653  EDVRVIQEFIELGNXXXXXXXXXXXXXXALGEIPDEFLDPIQYTLMEDPVILPSSRITVD 474
            ED R+IQEFI+LG                LGEIPDEFLDPIQYTLM+DPVILPSSRITVD
Sbjct: 910  EDGRIIQEFIQLGAKAKVAASEAMDTEATLGEIPDEFLDPIQYTLMKDPVILPSSRITVD 969

Query: 473  RPVIQRHLLSDSTDPFNRSHLTADMLIPDTELKAQINEFLRSRELNRHGDNLSMQSTKDT 294
            RPVIQRHLLSDSTDPFNRSHLTADMLIPD ELKA+I EF+RS+E+ +H   LS+QSTK T
Sbjct: 970  RPVIQRHLLSDSTDPFNRSHLTADMLIPDDELKARIEEFVRSQEMKKH---LSLQSTKAT 1026

Query: 293  IQTTD-EMPLID 261
            IQTT+ E  LID
Sbjct: 1027 IQTTNGETMLID 1038



 Score =  179 bits (454), Expect = 5e-42
 Identities = 102/175 (58%), Positives = 125/175 (71%), Gaps = 7/175 (4%)
 Frame = -1

Query: 3415 MATTKPQRSPAEIEDIILRKIFLVSLQDTMEND----SRIVYLEMTAAEILSEGGDLRLS 3248
            MA  KPQR+P E+EDII+RKIFLVS+ +   ++    S+IVYLE+TAAEILSEG +LRLS
Sbjct: 1    MAAAKPQRTPQEVEDIIIRKIFLVSITEIANSNTTTNSKIVYLELTAAEILSEGKELRLS 60

Query: 3247 RDLMERVLIDRLSGNFITA--EPPFIYLLGCYRRAYDEGKKVLSMKDKSLSADMVEVTKQ 3074
            RD MERVLIDRLSG F  A  E PF YL+GCY RA++EGKK+ +MKDK+L ++M  V +Q
Sbjct: 61   RDCMERVLIDRLSGEFAGAGDESPFQYLVGCYHRAHEEGKKISNMKDKNLRSEMETVVRQ 120

Query: 3073 AKKLAVSYCRIHLGNPDMFPXXXXXXXXXXXXXXSQLLPLIFSEV-SSTVDGFGG 2912
            AKKL V+YCRIHL NP++FP              S LL LIF+EV    V G GG
Sbjct: 121  AKKLCVNYCRIHLANPELFP----SRGSASTGANSPLLLLIFAEVGGGNVFGGGG 171


>ref|XP_003554717.1| PREDICTED: probable ubiquitin conjugation factor E4-like [Glycine
            max]
          Length = 1036

 Score = 1323 bits (3425), Expect = 0.0
 Identities = 671/853 (78%), Positives = 729/853 (85%), Gaps = 14/853 (1%)
 Frame = -3

Query: 2777 DFDSVDPILKQLYEDLRGSVLTVSALGNFQQPLRALLYLVNTPAGAKSLVSHHWWIPKGA 2598
            DFDS+D ILK LYE+LRGSV+ VSALGNFQ  LRALLYLV  P GAKSLV+H WWIPKG 
Sbjct: 187  DFDSLDKILKGLYEELRGSVMKVSALGNFQDSLRALLYLVRFPVGAKSLVNHEWWIPKGV 246

Query: 2597 YLNGRVIEMTSILGPFFHVSALPDQTIFRSQPDVGQQCFAEASTRRPADLLSSFTTIRTV 2418
            Y+NGR IEMTSILGPFFH+SALPD   F+ QPDVGQQCF++ASTRRPADLLSSF+TI+TV
Sbjct: 247  YMNGRAIEMTSILGPFFHISALPDHAFFKGQPDVGQQCFSDASTRRPADLLSSFSTIKTV 306

Query: 2417 MNNLYDGLAEVLKCLLKNTNTRENVLEYLAEVINKNKSRSHIQVDPISCASSGMFVNLSA 2238
            MNNLYDGLAEVL  LLK+ +TRE+VLEYLAE IN N SR+HIQVDPI+CASSGMFVNLSA
Sbjct: 307  MNNLYDGLAEVLLILLKSQDTRESVLEYLAEAININASRAHIQVDPITCASSGMFVNLSA 366

Query: 2237 VMLRLCEPFLDANLTKRDKIDSKYVFNSSRLDLRELTALHATSEEVAEWFTKNNPGKVDI 2058
            VMLRLCEPFLDANLTKRDKID+KYV  S+RL L  LTALHA+SEEV EW    NP     
Sbjct: 367  VMLRLCEPFLDANLTKRDKIDAKYVHCSNRLKLSGLTALHASSEEVTEWLNSKNPATTGA 426

Query: 2057 NSTSNDGENRL-------------LXXXXXXXXXXXXXAKYPFICECFFMTARVLNLGLL 1917
             +  +D + RL                            KY FICECFFMTARVLNLGLL
Sbjct: 427  TNQYSDDQKRLQQSQEASSSGSNNFGELSNENSARAEKTKYSFICECFFMTARVLNLGLL 486

Query: 1916 KGFSDFKHLVQDIQRSEDSLTSLKAMQEQAPSPQLNQDITRLEKELELYSQEKLCYEAQI 1737
            K FSDFKHLVQDI R ED+L +LKAMQE+ P+PQ   DI RLEKE+ELYSQEKLCYEAQI
Sbjct: 487  KAFSDFKHLVQDISRCEDALATLKAMQERTPTPQAELDINRLEKEMELYSQEKLCYEAQI 546

Query: 1736 LRDGGFLQQALSFYRLMVVWLARLVGGFKMPLPPTCPREFASMPEHFVEDVMEFLIFASR 1557
            LRD   +Q ALS YRLM++WL  LVGGFKMPLPPTCP EFA+MPEHFVED ME LIFASR
Sbjct: 547  LRDNTLIQNALSLYRLMIIWLVGLVGGFKMPLPPTCPMEFATMPEHFVEDAMELLIFASR 606

Query: 1556 IPKALDGAMLDDFMNFIIMFMGSPEYIKNPYLRAKMVEVLNCWMPRRSGSSATSTLFEGH 1377
            IPKALDG +L++FMNFIIMFM SPE+IKNPYLRAKMVEVLNCWMPRRSGS+AT+TLFEGH
Sbjct: 607  IPKALDGVVLEEFMNFIIMFMASPEFIKNPYLRAKMVEVLNCWMPRRSGSTATATLFEGH 666

Query: 1376 QLSLEYLVKNLLKVYVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKE 1197
            QLSLEYLV+NLLK+YVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKE
Sbjct: 667  QLSLEYLVRNLLKLYVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKE 726

Query: 1196 EEKGVYLNFLNFLVNDSIYLLDESLNKILELKELEAEMSNTVEWERRPSQERQERTRLFH 1017
            EEKGVYLNFLNFL+NDSIYLLDESLNKILELKELEAEMSNTVEWERRP QERQERTRLFH
Sbjct: 727  EEKGVYLNFLNFLINDSIYLLDESLNKILELKELEAEMSNTVEWERRPVQERQERTRLFH 786

Query: 1016 SQENIIRIDMKLANEDVTMLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVGPQRKSLS 837
            SQENIIRIDMKLANEDV+MLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVGPQRKSLS
Sbjct: 787  SQENIIRIDMKLANEDVSMLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVGPQRKSLS 846

Query: 836  LKDPEKYEFRPKHLLKQIVNIYVHLARGDKHNIFPSAITKDGRSYNEQLFSAAASVLQRI 657
            LKDPEKYEFRPKHLLKQIV+IYVHLARGD ++IFP+AI+KDGRSYN+QLFSA A VL RI
Sbjct: 847  LKDPEKYEFRPKHLLKQIVHIYVHLARGDTNSIFPAAISKDGRSYNDQLFSAGADVLHRI 906

Query: 656  GEDVRVIQEFIELGNXXXXXXXXXXXXXXALGEIPDEFLDPIQYTLMEDPVILPSSRITV 477
            GED R+IQEFI+LG                LGEIPDEFLDPIQYTLM+DPVILPSSRITV
Sbjct: 907  GEDGRIIQEFIQLGAKAKVAASEAMDAEATLGEIPDEFLDPIQYTLMKDPVILPSSRITV 966

Query: 476  DRPVIQRHLLSDSTDPFNRSHLTADMLIPDTELKAQINEFLRSRELNRHGDNLSMQSTKD 297
            DRPVIQRHLLSDSTDPFNRSHLTADMLIPD  LKA+I EF+RS+E+ +H   LS+QSTK 
Sbjct: 967  DRPVIQRHLLSDSTDPFNRSHLTADMLIPDDALKARIEEFVRSQEMKKH---LSLQSTKA 1023

Query: 296  TIQTTD-EMPLID 261
            TIQTT+ E  L+D
Sbjct: 1024 TIQTTNGETMLVD 1036



 Score =  179 bits (453), Expect = 6e-42
 Identities = 101/172 (58%), Positives = 122/172 (70%), Gaps = 4/172 (2%)
 Frame = -1

Query: 3415 MATTKPQRSPAEIEDIILRKIFLVSLQD-TMENDSRIVYLEMTAAEILSEGGDLRLSRDL 3239
            MA  KPQR+P E+EDI++RKIFLVS+ +     DSRIVYLE+TAAEILSE  +LRLSRD 
Sbjct: 1    MAAAKPQRTPQEVEDIVIRKIFLVSITEIATTTDSRIVYLELTAAEILSEDKELRLSRDC 60

Query: 3238 MERVLIDRLSGNFITA--EPPFIYLLGCYRRAYDEGKKVLSMKDKSLSADMVEVTKQAKK 3065
            MERVLIDRLSG F  A  E PF YL+GCY RA++EGKK+ +MKDK+L ++M  V +QAKK
Sbjct: 61   MERVLIDRLSGEFAGAVDESPFQYLVGCYHRAHEEGKKIANMKDKTLRSEMEAVVRQAKK 120

Query: 3064 LAVSYCRIHLGNPDMFPXXXXXXXXXXXXXXSQLLPLIFSEV-SSTVDGFGG 2912
            L V+YCRIHL NP++FP              S LL LI +EV    V G GG
Sbjct: 121  LCVNYCRIHLANPELFP---SRGSANSGGANSPLLSLILAEVGGGNVFGGGG 169


>ref|XP_002324089.1| predicted protein [Populus trichocarpa] gi|222867091|gb|EEF04222.1|
            predicted protein [Populus trichocarpa]
          Length = 1019

 Score = 1316 bits (3405), Expect = 0.0
 Identities = 656/840 (78%), Positives = 733/840 (87%), Gaps = 1/840 (0%)
 Frame = -3

Query: 2777 DFDSVDPILKQLYEDLRGSVLTVSALGNFQQPLRALLYLVNTPAGAKSLVSHHWWIPKGA 2598
            D DS+DPI K LYEDLRG+VL VS LGNFQQPLRALL+LV+   GAKSLV H WWIP GA
Sbjct: 182  DLDSLDPIFKGLYEDLRGNVLKVSVLGNFQQPLRALLFLVSFTVGAKSLVGHKWWIPTGA 241

Query: 2597 YLNGRVIEMTSILGPFFHVSALPDQTIFRSQPDVGQQCFAEASTRRPADLLSSFTTIRTV 2418
            Y+NGRVIEMTSILGPFFHVSALPD TIF+S+PDVGQQCF++A+ RR ADLLSSFTTI+T+
Sbjct: 242  YVNGRVIEMTSILGPFFHVSALPDNTIFKSEPDVGQQCFSDATNRRQADLLSSFTTIKTL 301

Query: 2417 MNNLYDGLAEVLKCLLKNTNTRENVLEYLAEVINKNKSRSHIQVDPISCASSGMFVNLSA 2238
            MN+LYDGL+EVL  LLKN++TRE+VL+YLAEVIN+N +R+HIQVDP+SCASSGMFVNLSA
Sbjct: 302  MNHLYDGLSEVLLALLKNSDTRESVLQYLAEVINRNATRAHIQVDPLSCASSGMFVNLSA 361

Query: 2237 VMLRLCEPFLDANLTKRDKIDSKYVFNSSRLDLRELTALHATSEEVAEWFTKNNPGKVDI 2058
            VMLRL EPFLDANL+K+DKID  YVF ++RLD+R LTALHA+SEE+ EW   N P K D+
Sbjct: 362  VMLRLSEPFLDANLSKKDKIDPNYVFQNNRLDIRGLTALHASSEEITEWL--NTPRKTDV 419

Query: 2057 NSTSNDGENRLLXXXXXXXXXXXXXA-KYPFICECFFMTARVLNLGLLKGFSDFKHLVQD 1881
            ++ S+D ENRLL               KY FICECFFMTARVLNLGLLK FSDFKHLVQD
Sbjct: 420  SALSSDEENRLLQSQEASSSGNSGEKAKYSFICECFFMTARVLNLGLLKAFSDFKHLVQD 479

Query: 1880 IQRSEDSLTSLKAMQEQAPSPQLNQDITRLEKELELYSQEKLCYEAQILRDGGFLQQALS 1701
            I R ED+L++ KA+Q+Q PSPQL  DI RLEKE+ELYSQEKLCYEAQILRDG  +Q ALS
Sbjct: 480  ISRCEDTLSTFKALQKQTPSPQLQLDIDRLEKEIELYSQEKLCYEAQILRDGALIQHALS 539

Query: 1700 FYRLMVVWLARLVGGFKMPLPPTCPREFASMPEHFVEDVMEFLIFASRIPKALDGAMLDD 1521
            FYRLM+VWL  LVGGFKMPLP TCP+EFASMPEHFVED ME LIFASRIPKALDG +LDD
Sbjct: 540  FYRLMLVWLVNLVGGFKMPLPLTCPKEFASMPEHFVEDAMELLIFASRIPKALDGVLLDD 599

Query: 1520 FMNFIIMFMGSPEYIKNPYLRAKMVEVLNCWMPRRSGSSATSTLFEGHQLSLEYLVKNLL 1341
            FMNFIIMFM SP YI+NPYLRAKMVEVLNCWMPRRSGSSAT++LFEGH LSLEYLV+NLL
Sbjct: 600  FMNFIIMFMASPTYIRNPYLRAKMVEVLNCWMPRRSGSSATASLFEGHHLSLEYLVRNLL 659

Query: 1340 KVYVDIEFTGSHTQFYDKFNIRHNIAELLEYLWQVPSHRNAWRQIAKEEEKGVYLNFLNF 1161
            K+YVDIEFTGSHTQF+DKFNIRHNIAELLEYLWQVPSHRN W +IAKEEEKGVYL FLNF
Sbjct: 660  KLYVDIEFTGSHTQFFDKFNIRHNIAELLEYLWQVPSHRNIWMKIAKEEEKGVYLKFLNF 719

Query: 1160 LVNDSIYLLDESLNKILELKELEAEMSNTVEWERRPSQERQERTRLFHSQENIIRIDMKL 981
            L+NDSIYLLDESLNKILE+K LEAEMSNT EWERRP+QERQERTRLFHSQENIIRIDMKL
Sbjct: 720  LINDSIYLLDESLNKILEIKGLEAEMSNTTEWERRPAQERQERTRLFHSQENIIRIDMKL 779

Query: 980  ANEDVTMLAFTSEQITAPFLLPEMVERVASMLNYFLLQLVGPQRKSLSLKDPEKYEFRPK 801
            ANEDV+ML FTSEQITAPFLLPEMV+RVA+MLNYFLLQLVGPQR+SL+LKDPEKYEFRPK
Sbjct: 780  ANEDVSMLTFTSEQITAPFLLPEMVDRVATMLNYFLLQLVGPQRRSLTLKDPEKYEFRPK 839

Query: 800  HLLKQIVNIYVHLARGDKHNIFPSAITKDGRSYNEQLFSAAASVLQRIGEDVRVIQEFIE 621
             LLKQIV+IYVHLARGD  NIFP+AI KDGRSYNEQLF+AAA VL+RIGED RV+QEFIE
Sbjct: 840  QLLKQIVHIYVHLARGDTENIFPAAILKDGRSYNEQLFTAAADVLRRIGEDGRVVQEFIE 899

Query: 620  LGNXXXXXXXXXXXXXXALGEIPDEFLDPIQYTLMEDPVILPSSRITVDRPVIQRHLLSD 441
            LG                LGE+P+EFLDPIQ TLM+DPVILPSSR TVDRPVI RHLLSD
Sbjct: 900  LGTKTKVAASEAMDAEVTLGEVPEEFLDPIQCTLMKDPVILPSSRTTVDRPVILRHLLSD 959

Query: 440  STDPFNRSHLTADMLIPDTELKAQINEFLRSRELNRHGDNLSMQSTKDTIQTTDEMPLID 261
            +TDPFNRSHLT DMLI +TELKA+I+E++RS+EL RHG++ S+Q  K+TIQTT E  LID
Sbjct: 960  NTDPFNRSHLTVDMLISNTELKARIDEYIRSQELKRHGEDFSLQRAKETIQTTTEEMLID 1019



 Score =  176 bits (445), Expect = 5e-41
 Identities = 101/168 (60%), Positives = 121/168 (72%), Gaps = 2/168 (1%)
 Frame = -1

Query: 3415 MATT--KPQRSPAEIEDIILRKIFLVSLQDTMENDSRIVYLEMTAAEILSEGGDLRLSRD 3242
            MATT  KPQRS  EIEDII+RKI L+SL D+  +D RI+YLEMTAAEILSEG DL+L+RD
Sbjct: 1    MATTSNKPQRSLEEIEDIIVRKILLISLTDS--SDPRIIYLEMTAAEILSEGKDLKLNRD 58

Query: 3241 LMERVLIDRLSGNFITAEPPFIYLLGCYRRAYDEGKKVLSMKDKSLSADMVEVTKQAKKL 3062
            L+ERVLIDRLS     AEPPF YLLGCYRRA DE KK+ +MKDK + +++    +Q KKL
Sbjct: 59   LIERVLIDRLSVQNPNAEPPFNYLLGCYRRAVDELKKIANMKDKIVKSELELSIRQLKKL 118

Query: 3061 AVSYCRIHLGNPDMFPXXXXXXXXXXXXXXSQLLPLIFSEVSSTVDGF 2918
            +VSYCRIHLGNP++F               S +LPLIF    + VDGF
Sbjct: 119  SVSYCRIHLGNPELFGDDSNVVKGSGNSNVSPVLPLIF----AMVDGF 162