BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00004056
         (1141 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002266697.1| PREDICTED: NAD-dependent malic enzyme 59 kDa...   446   e-123
ref|XP_002511819.1| malic enzyme, putative [Ricinus communis] gi...   436   e-120
sp|P37225.1|MAON_SOLTU RecName: Full=NAD-dependent malic enzyme ...   426   e-117
ref|XP_004163101.1| PREDICTED: NAD-dependent malic enzyme 2, mit...   425   e-117
ref|XP_003521187.1| PREDICTED: NAD-dependent malic enzyme 59 kDa...   422   e-115

>ref|XP_002266697.1| PREDICTED: NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
            [Vitis vinifera] gi|297745211|emb|CBI40291.3| unnamed
            protein product [Vitis vinifera]
          Length = 605

 Score =  446 bits (1147), Expect = e-123
 Identities = 234/314 (74%), Positives = 250/314 (79%), Gaps = 3/314 (0%)
 Frame = +1

Query: 1    FNDDIQXXXXXXXXXXXXXXRAQGRPLTDFANQKIXXXXXXXXXXXXXNMAIQAVLRIAG 180
            FNDDIQ              RAQGRPLTDF NQKI             NMA QA  RIAG
Sbjct: 294  FNDDIQGTAGVALAGLLGTVRAQGRPLTDFVNQKIVVVGAGSAGIGVLNMAAQAASRIAG 353

Query: 181  S-GADPH--FFLLDKDGLLTKERKGIDPAAAPFAKSLAETEEMGLREGASLVEVVKKVKP 351
            + GA P+  F+LLDKDGL+TKERK IDPAAAPFAK   E E  GLREGASL+EVVKKVKP
Sbjct: 354  NTGASPNHQFYLLDKDGLITKERKNIDPAAAPFAKGPGEIE--GLREGASLLEVVKKVKP 411

Query: 352  HXXXXXXXXXXXFHEEVLKAMRESDSTKPAIFAMSNPTTKAECTAVDAFKHAGENIVFAS 531
            H           F+EEVLKAMRESDSTKPAIFAMSNPT  AECTA DAFKHAGENIVFAS
Sbjct: 412  HVLLGLSGVGGVFNEEVLKAMRESDSTKPAIFAMSNPTMNAECTAADAFKHAGENIVFAS 471

Query: 532  GSPFENVDLGNGKVGHVNQANNMYLFPGIGLGTLLSGAHYISDGMLQAASECLASYMKDE 711
            GSPF NV+LGNGKVGHVNQANNMYLFPGIGLGTLL+GAH+ISDGMLQAA+ECLASYM DE
Sbjct: 472  GSPFANVNLGNGKVGHVNQANNMYLFPGIGLGTLLAGAHFISDGMLQAAAECLASYMSDE 531

Query: 712  EIQMGILYPSISSIREITAEVGAAVLRAAIAEGLAEGHGDVGPRELEHMSEEETIEYVRR 891
            E Q GILYPSI SIR ITAEVGAAVLRAA+AE LAEGHGDVGPRELEHMS+EET+EY+ R
Sbjct: 532  ETQNGILYPSIDSIRHITAEVGAAVLRAAVAEELAEGHGDVGPRELEHMSKEETVEYIIR 591

Query: 892  NMWYPVYSPLVHEK 933
            NMW+P+YSPLVHEK
Sbjct: 592  NMWFPIYSPLVHEK 605


>ref|XP_002511819.1| malic enzyme, putative [Ricinus communis] gi|223548999|gb|EEF50488.1|
            malic enzyme, putative [Ricinus communis]
          Length = 602

 Score =  436 bits (1120), Expect = e-120
 Identities = 225/314 (71%), Positives = 249/314 (79%), Gaps = 3/314 (0%)
 Frame = +1

Query: 1    FNDDIQXXXXXXXXXXXXXXRAQGRPLTDFANQKIXXXXXXXXXXXXXNMAIQAVLRIAG 180
            FNDDIQ              RAQGRPL+DF  QKI             NMAIQAV R++G
Sbjct: 291  FNDDIQGTAGVALAGLLGTVRAQGRPLSDFVKQKIVVVGAGSAGLGVLNMAIQAVSRMSG 350

Query: 181  SG---ADPHFFLLDKDGLLTKERKGIDPAAAPFAKSLAETEEMGLREGASLVEVVKKVKP 351
            +     + +FFLLDKDGL+TKERK IDPAAAPFAK L + E  GLREGA+LVEVVKK+KP
Sbjct: 351  NNEASVNNNFFLLDKDGLITKERKNIDPAAAPFAKDLKDVE--GLREGATLVEVVKKLKP 408

Query: 352  HXXXXXXXXXXXFHEEVLKAMRESDSTKPAIFAMSNPTTKAECTAVDAFKHAGENIVFAS 531
            H           F++EVLKAMR+SD  KPAIFAMSNPT  AECTA DAFKHAGENIVFAS
Sbjct: 409  HVLLGLSGVGGIFNDEVLKAMRDSDCIKPAIFAMSNPTMNAECTATDAFKHAGENIVFAS 468

Query: 532  GSPFENVDLGNGKVGHVNQANNMYLFPGIGLGTLLSGAHYISDGMLQAASECLASYMKDE 711
            GSPFENVDLGNGKVGHVNQANNMYLFPGIGLG L+SGA +I+DGMLQAA+ECLASYM DE
Sbjct: 469  GSPFENVDLGNGKVGHVNQANNMYLFPGIGLGALVSGARFITDGMLQAAAECLASYMTDE 528

Query: 712  EIQMGILYPSISSIREITAEVGAAVLRAAIAEGLAEGHGDVGPRELEHMSEEETIEYVRR 891
            EIQ GILYPSI+SIR ITAEVGAAVLRAA+AE LAEGHGDVGPREL+HMS+EET+EYV R
Sbjct: 529  EIQKGILYPSINSIRHITAEVGAAVLRAAVAEHLAEGHGDVGPRELKHMSKEETVEYVTR 588

Query: 892  NMWYPVYSPLVHEK 933
            NMW+P+YSPLVHEK
Sbjct: 589  NMWFPIYSPLVHEK 602


>sp|P37225.1|MAON_SOLTU RecName: Full=NAD-dependent malic enzyme 59 kDa isoform,
            mitochondrial; Short=NAD-ME; Flags: Precursor
            gi|438131|emb|CAA80547.1| precursor of the 59kDa subunit
            of the mitochondrial NAD+-dependent malic enzyme [Solanum
            tuberosum]
          Length = 601

 Score =  426 bits (1095), Expect = e-117
 Identities = 217/310 (70%), Positives = 240/310 (77%)
 Frame = +1

Query: 1    FNDDIQXXXXXXXXXXXXXXRAQGRPLTDFANQKIXXXXXXXXXXXXXNMAIQAVLRIAG 180
            FNDDIQ              RAQGRPLTDFANQKI              MA+QAV R+ G
Sbjct: 292  FNDDIQGTAGVALAGLLGTVRAQGRPLTDFANQKIVVVGAGSAGLGVLKMALQAVSRMTG 351

Query: 181  SGADPHFFLLDKDGLLTKERKGIDPAAAPFAKSLAETEEMGLREGASLVEVVKKVKPHXX 360
              ADPHFFLLDK+GL+TK+RK IDPAA PFAK+  E E +GL+EGA L EVVKKVKPH  
Sbjct: 352  PSADPHFFLLDKNGLITKDRKDIDPAALPFAKAHHEIEGLGLQEGAGLAEVVKKVKPHVL 411

Query: 361  XXXXXXXXXFHEEVLKAMRESDSTKPAIFAMSNPTTKAECTAVDAFKHAGENIVFASGSP 540
                     FHEEVL+AM+ESDS +PAIFAMSNPT  AEC  VDAFK AGE+IVFASGSP
Sbjct: 412  LGLSGVGGIFHEEVLRAMKESDSVRPAIFAMSNPTNNAECCPVDAFKLAGEDIVFASGSP 471

Query: 541  FENVDLGNGKVGHVNQANNMYLFPGIGLGTLLSGAHYISDGMLQAASECLASYMKDEEIQ 720
            F NVDLGNGK+GHVNQANNMYLFPGIGLG LLSGA  ISD ML+AA+ECLASYM D+EI 
Sbjct: 472  FANVDLGNGKIGHVNQANNMYLFPGIGLGALLSGARNISDTMLEAAAECLASYMSDDEIN 531

Query: 721  MGILYPSISSIREITAEVGAAVLRAAIAEGLAEGHGDVGPRELEHMSEEETIEYVRRNMW 900
             GILYPSI  IR+ITAEVGAAVLRAA+AE LAEGHGDVG +EL+HMS+EETIE+VR+NMW
Sbjct: 532  RGILYPSIDDIRDITAEVGAAVLRAAVAEDLAEGHGDVGVKELQHMSKEETIEHVRQNMW 591

Query: 901  YPVYSPLVHE 930
            YPVY PLVHE
Sbjct: 592  YPVYGPLVHE 601


>ref|XP_004163101.1| PREDICTED: NAD-dependent malic enzyme 2, mitochondrial-like [Cucumis
            sativus]
          Length = 500

 Score =  425 bits (1093), Expect = e-117
 Identities = 222/314 (70%), Positives = 246/314 (78%), Gaps = 3/314 (0%)
 Frame = +1

Query: 1    FNDDIQXXXXXXXXXXXXXXRAQGRPLTDFANQKIXXXXXXXXXXXXXNMAIQAVLRIAG 180
            FNDDIQ              RAQGRPL+DF NQKI             NMAIQAV R+AG
Sbjct: 189  FNDDIQGTAGVALAGLLGTVRAQGRPLSDFVNQKIVVVGAGSAGLGVLNMAIQAVSRMAG 248

Query: 181  ---SGADPHFFLLDKDGLLTKERKGIDPAAAPFAKSLAETEEMGLREGASLVEVVKKVKP 351
               S A   FFL+DKDGL+TKER  IDPAAAPFAK   E E  GL EGA+L+EVVK+V+P
Sbjct: 249  NNDSTARSQFFLIDKDGLITKERTNIDPAAAPFAKDPRELE--GLSEGANLLEVVKRVRP 306

Query: 352  HXXXXXXXXXXXFHEEVLKAMRESDSTKPAIFAMSNPTTKAECTAVDAFKHAGENIVFAS 531
            H           F+EEVLKAMRESDS+KPAIFAMSNPT  AECTA DAFK+AGENIVFAS
Sbjct: 307  HVLLGLSGVGGIFNEEVLKAMRESDSSKPAIFAMSNPTMNAECTAADAFKYAGENIVFAS 366

Query: 532  GSPFENVDLGNGKVGHVNQANNMYLFPGIGLGTLLSGAHYISDGMLQAASECLASYMKDE 711
            GSPFENV LGNGK GHVNQANNMYLFPGIGLG+LLSGA YI+DGMLQAA+ECLASYM DE
Sbjct: 367  GSPFENVALGNGKFGHVNQANNMYLFPGIGLGSLLSGARYITDGMLQAAAECLASYMTDE 426

Query: 712  EIQMGILYPSISSIREITAEVGAAVLRAAIAEGLAEGHGDVGPRELEHMSEEETIEYVRR 891
            E+Q GILYPSI SIREITAEVGAAVLR+A++E LAEGHGDVGPREL  M++EETIEY++R
Sbjct: 427  EVQSGILYPSIDSIREITAEVGAAVLRSAVSENLAEGHGDVGPRELGLMAKEETIEYIKR 486

Query: 892  NMWYPVYSPLVHEK 933
            NMW+P+YSPLVHEK
Sbjct: 487  NMWFPIYSPLVHEK 500


>ref|XP_003521187.1| PREDICTED: NAD-dependent malic enzyme 59 kDa isoform,
            mitochondrial-like [Glycine max]
          Length = 604

 Score =  422 bits (1084), Expect = e-115
 Identities = 216/314 (68%), Positives = 242/314 (77%), Gaps = 3/314 (0%)
 Frame = +1

Query: 1    FNDDIQXXXXXXXXXXXXXXRAQGRPLTDFANQKIXXXXXXXXXXXXXNMAIQAVLRIAG 180
            FNDDIQ              RAQGRPLTDF NQKI              MAIQ V +I+G
Sbjct: 293  FNDDIQGTAGVALAGILGTVRAQGRPLTDFVNQKIVVVGAGSAGLGVLKMAIQTVAKISG 352

Query: 181  SG---ADPHFFLLDKDGLLTKERKGIDPAAAPFAKSLAETEEMGLREGASLVEVVKKVKP 351
                 A   F+L+DKDGL+T ER  +DPAAAPFAK+  + E  GL EGAS++EVVKK++P
Sbjct: 353  CSELAAKSQFYLIDKDGLVTTERNSLDPAAAPFAKNPRDIE--GLTEGASIIEVVKKIRP 410

Query: 352  HXXXXXXXXXXXFHEEVLKAMRESDSTKPAIFAMSNPTTKAECTAVDAFKHAGENIVFAS 531
            H           F+EEVLKAMRES STKPAIFAMSNPT  AECT++DAFKHAGENIVFAS
Sbjct: 411  HVLLGLSGVGGIFNEEVLKAMRESVSTKPAIFAMSNPTMNAECTSIDAFKHAGENIVFAS 470

Query: 532  GSPFENVDLGNGKVGHVNQANNMYLFPGIGLGTLLSGAHYISDGMLQAASECLASYMKDE 711
            GSPFENVDLGNGKVGHVNQANNMYLFPGIGLGTLLSGAH I+DGMLQAASECLASYM +E
Sbjct: 471  GSPFENVDLGNGKVGHVNQANNMYLFPGIGLGTLLSGAHLITDGMLQAASECLASYMAEE 530

Query: 712  EIQMGILYPSISSIREITAEVGAAVLRAAIAEGLAEGHGDVGPRELEHMSEEETIEYVRR 891
            +I  GILYPS+ SIR++TAEVGAAVLRAA+ E LAEGHGDVGP+EL HMS++E +EYVR 
Sbjct: 531  DILKGILYPSVDSIRDVTAEVGAAVLRAAVEEELAEGHGDVGPKELSHMSKDEAVEYVRS 590

Query: 892  NMWYPVYSPLVHEK 933
            NMWYPVYSPLVHEK
Sbjct: 591  NMWYPVYSPLVHEK 604