BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003460
         (1384 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002517867.1| conserved hypothetical protein [Ricinus comm...   344   3e-92
ref|XP_002275156.2| PREDICTED: reticulon-like protein B17-like [...   335   2e-89
ref|XP_002309754.1| predicted protein [Populus trichocarpa] gi|2...   320   4e-85
ref|NP_179649.2| reticulon-like protein B17 [Arabidopsis thalian...   306   8e-81
gb|AAO37135.1| hypothetical protein [Arabidopsis thaliana]            304   3e-80

>ref|XP_002517867.1| conserved hypothetical protein [Ricinus communis]
            gi|223542849|gb|EEF44385.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 444

 Score =  344 bits (883), Expect = 3e-92
 Identities = 203/409 (49%), Positives = 258/409 (63%), Gaps = 19/409 (4%)
 Frame = -2

Query: 1176 SPPPYHRSDPXXXXXXXXXXXRINQI--EEQSPNTSLELVIS-SPKL--SSPINTI---S 1021
            S PP HRS+P           RI      E+S  +SL+L++S +PK   S+P N      
Sbjct: 3    STPPSHRSNPNSQTKSASRLARITSSIDNEESLRSSLDLILSPAPKRTPSTPSNLSLKSC 62

Query: 1020 PKPYKLHEMLLLSPSPHRKSRTRIAEKLDFADQ---EPEGAXXXXXXXXXXXXXXXXXXX 850
                  HE+LLLSPSP RKSRTR+A++LD A++   EP G+                   
Sbjct: 63   TNSLPFHELLLLSPSPSRKSRTRLADRLDIAEEAAMEPTGSRRRCKSRTAQLGSPRISRR 122

Query: 849  XXXXXXKFEQEVREDRDL-GVLEDVGKPKKRRNSGRSKKEKMPTVSHT-------KLEDG 694
                    E E+RE++DL G++E++GK +KRR+SGRSKKEK+  V          K+ D 
Sbjct: 123  SRRRS---EMEIREEKDLLGLVEEIGKARKRRHSGRSKKEKLSLVPSLPSSNPSPKVGDC 179

Query: 693  GGGSCNFDRIGQLISDLVMWKDVARSTLWFGFGSLCILSSCFTTGVSFSIFSLISQLASV 514
             GG  N +RIG++I DL+MWKDVA+S+LWFGFG LC LSSCF  G+SFSIFS ISQL  +
Sbjct: 180  DGG--NLNRIGRMIYDLIMWKDVAKSSLWFGFGCLCFLSSCFAKGISFSIFSAISQLGLL 237

Query: 513  ALGVRFFSNSICKRDNGDENKRDIRLREEDXXXXXXXXXXXXXXXISKVGELFSGDPSTT 334
             LG  F SNSIC+R+N  E++ + +L+EED               IS   ELFSG+PS T
Sbjct: 238  FLGASFISNSICQRNNV-ESRCNFKLKEEDILKLGRLILPPANLAISMARELFSGEPSMT 296

Query: 333  LKVIPFLLLGSEYGHLITLWRLCAVGFLISFSVPKLYSSYSFQINEKVKFYTSRVLVTWG 154
            LKVIPFLL+G+EYGHLIT+WRLCA+GF I F++PKLYS YS QIN+KV +   RVL  W 
Sbjct: 297  LKVIPFLLVGAEYGHLITMWRLCAIGFFIGFTIPKLYSCYSSQINQKVDYARGRVLDAWE 356

Query: 153  ALSHKKIVGLSAATAFWNLTSVRTRFFTAFIILVILRYRSQYLKPTTEE 7
            A SHKKIV  SA TAFWN++SV+TR F AFI LVILR   Q++ P  EE
Sbjct: 357  ACSHKKIVAASAITAFWNMSSVKTRIFAAFIFLVILRCCRQHMIPNQEE 405


>ref|XP_002275156.2| PREDICTED: reticulon-like protein B17-like [Vitis vinifera]
          Length = 429

 Score =  335 bits (859), Expect = 2e-89
 Identities = 187/399 (46%), Positives = 247/399 (61%), Gaps = 9/399 (2%)
 Frame = -2

Query: 1176 SPPPYHRSDPXXXXXXXXXXXRINQIEE---QSPNTSLELVISSPKLSSPINTISPK-PY 1009
            SPP  H+S+P           RI +I++   + P  SL+L   SP       T+SP  P 
Sbjct: 3    SPPLSHQSEPRSGTKSASRPARIGEIDQGGQERPKISLDLSTLSPS-----KTLSPSNPL 57

Query: 1008 KLHEMLLLSPSPHRKSRTRIAEKLDFADQEPEGA----XXXXXXXXXXXXXXXXXXXXXX 841
             LHE+LLLSPSP R+SRTR+ E++D  ++  E                            
Sbjct: 58   PLHELLLLSPSPLRRSRTRLTERIDMGEEPLEATGSRRRCKSRISQMGVLGCASPRNTRR 117

Query: 840  XXXKFEQEVREDRDLGVLEDVGKPKKRRNSGRSKKEKMPTV-SHTKLEDGGGGSCNFDRI 664
               + EQ+VRED+D G+ E++GK +KRR+SGRS+KEK+  V S            N  RI
Sbjct: 118  PRRRVEQDVREDKDFGMAEEMGKLRKRRHSGRSRKEKLSLVPSVPSSSSSPNDLSNLHRI 177

Query: 663  GQLISDLVMWKDVARSTLWFGFGSLCILSSCFTTGVSFSIFSLISQLASVALGVRFFSNS 484
            GQL++DL+MWKD ++S+ WFGFGS+C LSSCFT G+SFSIFS ISQL  + LG+ FFSNS
Sbjct: 178  GQLVTDLIMWKDASKSSFWFGFGSVCFLSSCFTRGISFSIFSAISQLGLLFLGLSFFSNS 237

Query: 483  ICKRDNGDENKRDIRLREEDXXXXXXXXXXXXXXXISKVGELFSGDPSTTLKVIPFLLLG 304
            + +RDN  E +R+ +L+E+D               ISK  +LFSG+PS TLKV PF L+G
Sbjct: 238  LYQRDN-VEKRREFKLKEDDILRVARAILPATNLAISKTIQLFSGEPSMTLKVAPFFLVG 296

Query: 303  SEYGHLITLWRLCAVGFLISFSVPKLYSSYSFQINEKVKFYTSRVLVTWGALSHKKIVGL 124
            +EYGHL+T+ RLCA+GF ISF++PKLYS YS QIN +V++    V+  WGA SHKKIV  
Sbjct: 297  AEYGHLLTVRRLCALGFFISFTIPKLYSCYSAQINTRVEYLKGWVVEAWGACSHKKIVAA 356

Query: 123  SAATAFWNLTSVRTRFFTAFIILVILRYRSQYLKPTTEE 7
            SA T FWNL++++TR F AFI LVILRY  Q  +   EE
Sbjct: 357  SAVTVFWNLSAIKTRIFAAFIALVILRYHRQQSEANMEE 395


>ref|XP_002309754.1| predicted protein [Populus trichocarpa] gi|222852657|gb|EEE90204.1|
            predicted protein [Populus trichocarpa]
          Length = 457

 Score =  320 bits (821), Expect = 4e-85
 Identities = 194/403 (48%), Positives = 247/403 (61%), Gaps = 15/403 (3%)
 Frame = -2

Query: 1170 PPYHRSDPXXXXXXXXXXXRINQI---EEQSPNTSLELVISSPKLS-SPINTISPKP--- 1012
            PP HRS+            RI      E + P  SL+L+ SSPK + S  +++S K    
Sbjct: 5    PPSHRSNSNTQAKSASRLSRITYSIDPESRPPQLSLDLIPSSPKETPSTPSSLSLKSSTN 64

Query: 1011 -YKLHEMLLLSPSPHRKSRTRIAEKLDFADQ---EPEGAXXXXXXXXXXXXXXXXXXXXX 844
               L E+LLLSPSP RKSRTR+A++ +  ++   E  G+                     
Sbjct: 65   FLPLRELLLLSPSPSRKSRTRLADRDEMPEEGGLEQNGSRRRCKTRGSQTGSLGCASPRS 124

Query: 843  XXXXK--FEQEVREDRDL-GVLEDVGKPKKRRNSGRSKKEKMP-TVSHTKLEDGGGGSCN 676
                +   E E RE+RDL G++++VGK +KRR+SGRSKKEK   ++ H+        S N
Sbjct: 125  NRRLRRRLEVESREERDLIGLVDEVGKVRKRRHSGRSKKEKEKLSLVHSFPSSNSTTSIN 184

Query: 675  FDRIGQLISDLVMWKDVARSTLWFGFGSLCILSSCFTTGVSFSIFSLISQLASVALGVRF 496
             DRIG ++ DL+MWKDVA+S+LWFG G LC LSSCF  G++FSI S ISQL  + LG  F
Sbjct: 185  LDRIGMVVYDLIMWKDVAKSSLWFGLGCLCFLSSCFAKGMNFSILSAISQLGLLVLGASF 244

Query: 495  FSNSICKRDNGDENKRDIRLREEDXXXXXXXXXXXXXXXISKVGELFSGDPSTTLKVIPF 316
             SNSIC+R+N  E  R  +L EED               ISK  ELFSG+PS TLKVIPF
Sbjct: 245  CSNSICQRNNV-EKTRKFKLTEEDVLRVGTLILPAANLAISKTRELFSGEPSMTLKVIPF 303

Query: 315  LLLGSEYGHLITLWRLCAVGFLISFSVPKLYSSYSFQINEKVKFYTSRVLVTWGALSHKK 136
            LLLG+EYGHL+TL RLCA+GF ISF++PKLY+ YS QI +K +   +R+   WGA SHKK
Sbjct: 304  LLLGAEYGHLVTLRRLCAIGFFISFTIPKLYACYSSQIKQKAEQMKNRMFEAWGACSHKK 363

Query: 135  IVGLSAATAFWNLTSVRTRFFTAFIILVILRYRSQYLKPTTEE 7
            IV  SA TAFWNL+SV+TR FTAFI LVILR   Q+L P  E+
Sbjct: 364  IVAASAVTAFWNLSSVKTRIFTAFISLVILRCFRQHLMPKQED 406


>ref|NP_179649.2| reticulon-like protein B17 [Arabidopsis thaliana]
            gi|75120766|sp|Q6DR04.1|RTNLQ_ARATH RecName:
            Full=Reticulon-like protein B17; Short=AtRTNLB17
            gi|50058967|gb|AAT69228.1| hypothetical protein At2g20590
            [Arabidopsis thaliana] gi|330251942|gb|AEC07036.1|
            reticulon-like protein B17 [Arabidopsis thaliana]
          Length = 431

 Score =  306 bits (784), Expect = 8e-81
 Identities = 177/394 (44%), Positives = 228/394 (57%), Gaps = 4/394 (1%)
 Frame = -2

Query: 1176 SPPPYHRSDPXXXXXXXXXXXRINQIEEQSPNTSLELVISSPKLSSPINTISPKPYKLHE 997
            S PPYHRS+               Q     PN SL+LV+SSP   +P +     P  L +
Sbjct: 3    STPPYHRSNTKSASRL--------QDSSNPPNLSLDLVLSSPNTPNPSS-----PVPLRD 49

Query: 996  MLLLSPSPHRKSRTRIAEKLDFADQEPEGAXXXXXXXXXXXXXXXXXXXXXXXXXKFEQE 817
            +LLL PSP RKSRTR++++L+   ++                             +  + 
Sbjct: 50   ILLLPPSPLRKSRTRLSDRLEMTSEDAMAVVRKRGKGKGGQKSLLASPRNPRRSRRRSEA 109

Query: 816  VREDRDLGVLEDVGK--PKKRRNSGRSKKEKMPTVSHTKLEDGGGGSCNFDR--IGQLIS 649
            V E     V+E++ K  P+KR+ +GR KK+K  +            +C  D   IG++IS
Sbjct: 110  VEEKEANLVIEEIVKLPPRKRKTNGRPKKDKQSSAPPLCSSSDLPNTCQSDLNLIGEIIS 169

Query: 648  DLVMWKDVARSTLWFGFGSLCILSSCFTTGVSFSIFSLISQLASVALGVRFFSNSICKRD 469
            DLVMW+DVA+STLWFGFG L  LSSCF  GV+FS+FS +S L  V L   F SN++C+R 
Sbjct: 170  DLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGLVLLCGSFLSNTLCQRK 229

Query: 468  NGDENKRDIRLREEDXXXXXXXXXXXXXXXISKVGELFSGDPSTTLKVIPFLLLGSEYGH 289
            N D  KR   + E+D               ISK  ELFSG+PS TLKV PFLLLG+EYGH
Sbjct: 230  NED-TKRAFHVSEDDVLRSARRVLPATNFFISKTSELFSGEPSMTLKVTPFLLLGAEYGH 288

Query: 288  LITLWRLCAVGFLISFSVPKLYSSYSFQINEKVKFYTSRVLVTWGALSHKKIVGLSAATA 109
            LITLWRL A GF +SF++PKLYS Y+ QI++KV+   +R+   WG  SHKKI+  SA TA
Sbjct: 289  LITLWRLSAFGFFLSFTIPKLYSCYTHQISQKVERVKTRIGEAWGVCSHKKILAGSAVTA 348

Query: 108  FWNLTSVRTRFFTAFIILVILRYRSQYLKPTTEE 7
            FWNLTS+RTR F  FIILVI RYR Q L+ T EE
Sbjct: 349  FWNLTSIRTRIFAVFIILVIFRYRRQNLQLTPEE 382


>gb|AAO37135.1| hypothetical protein [Arabidopsis thaliana]
          Length = 431

 Score =  304 bits (779), Expect = 3e-80
 Identities = 178/394 (45%), Positives = 228/394 (57%), Gaps = 4/394 (1%)
 Frame = -2

Query: 1176 SPPPYHRSDPXXXXXXXXXXXRINQIEEQSPNTSLELVISSPKLSSPINTISPKPYKLHE 997
            S PPYHRS+               Q     PN SL+LV+SSP   +P +     P  L +
Sbjct: 3    STPPYHRSNTKSASRL--------QDSSNPPNLSLDLVLSSPNTPNPSS-----PVPLRD 49

Query: 996  MLLLSPSPHRKSRTRIAEKLDFADQEPEGAXXXXXXXXXXXXXXXXXXXXXXXXXKFEQE 817
            +LLL PSP RKSRTR++++L+   ++                             +  + 
Sbjct: 50   ILLLPPSPLRKSRTRLSDRLEMTSEDAMAVVRKRGKGKGGQKSLLASPRNPRRSRRRSEA 109

Query: 816  VREDRDLGVLEDVGK--PKKRRNSGRSKKEKMPTVSHTKLEDGGGGSCNFDR--IGQLIS 649
            V E     V+E++ K  P KR+ +GR KK+K  +            +C  D   IG++IS
Sbjct: 110  VEEKEANLVIEEIVKLPPIKRKTNGRPKKDKQSSAPPLCSSSDLPNTCQSDLNLIGEIIS 169

Query: 648  DLVMWKDVARSTLWFGFGSLCILSSCFTTGVSFSIFSLISQLASVALGVRFFSNSICKRD 469
            DLVMW+DVA+STLWFGFG L  LSSCF  GV+FS+FS +S L  V L   F SN++C+R 
Sbjct: 170  DLVMWRDVAKSTLWFGFGCLSFLSSCFAKGVNFSVFSAVSNLGLVLLCGSFLSNTLCQRK 229

Query: 468  NGDENKRDIRLREEDXXXXXXXXXXXXXXXISKVGELFSGDPSTTLKVIPFLLLGSEYGH 289
            N D  KR   + E+D               ISK  ELFSG+PS TLKV PFLLLG+EYGH
Sbjct: 230  NED-TKRAFHVSEDDVLRSARRVLPATNXFISKTSELFSGEPSMTLKVTPFLLLGAEYGH 288

Query: 288  LITLWRLCAVGFLISFSVPKLYSSYSFQINEKVKFYTSRVLVTWGALSHKKIVGLSAATA 109
            LITLWRL A GF +SF++PKLYS Y+ QI++KV+   +R+   WG  SHKKI+  SA TA
Sbjct: 289  LITLWRLSAFGFFLSFTIPKLYSCYTHQISQKVERVKTRIGEAWGVCSHKKILAGSAVTA 348

Query: 108  FWNLTSVRTRFFTAFIILVILRYRSQYLKPTTEE 7
            FWNLTS+RTR F  FIILVI RYR Q L+ T EE
Sbjct: 349  FWNLTSIRTRIFAVFIILVIFRYRRQNLQLTPEE 382