BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003355
         (1142 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q7XZQ6.1|FLS_PETCR RecName: Full=Flavonol synthase/flavanone ...   619   e-175
sp|Q07512.1|FLS_PETHY RecName: Full=Flavonol synthase/flavanone ...   546   e-153
gb|ADZ28516.1| flavonol synthase [Camellia nitidissima]               544   e-152
gb|ACL98052.1| flavonol synthase [Camellia sinensis]                  542   e-152
gb|ABE28017.1| flavonol synthase [Nicotiana tabacum]                  541   e-152

>sp|Q7XZQ6.1|FLS_PETCR RecName: Full=Flavonol synthase/flavanone 3-hydroxylase
            gi|31978953|gb|AAP57395.1| flavonol synthase
            [Petroselinum crispum]
          Length = 337

 Score =  619 bits (1596), Expect = e-175
 Identities = 295/329 (89%), Positives = 314/329 (95%), Gaps = 2/329 (0%)
 Frame = +1

Query: 160  MEVERVQAISKMSKCMDTIPSEYIRSENEQPAVTTMQGVVLEVPVIDLGSPD--EENLVR 333
            MEVERVQAISKMS+CMDTIPSEYIRSE+EQPAVTTMQGVVL+VPVIDLGS +  EENLV 
Sbjct: 1    MEVERVQAISKMSRCMDTIPSEYIRSESEQPAVTTMQGVVLQVPVIDLGSSNNTEENLVE 60

Query: 334  LIADASREWGIFQVVNHGIPDEAIAKLQKVGKEFFELPQEEKEVIAKPEGYQGVEGYGTK 513
            LIA+ASREWGIFQVVNHGIPD+AIAKLQKVGKEFFELPQ+EKEVIAKPEGYQGVEGYGTK
Sbjct: 61   LIAEASREWGIFQVVNHGIPDDAIAKLQKVGKEFFELPQQEKEVIAKPEGYQGVEGYGTK 120

Query: 514  LQKEVGGKKGWVDHLFHIIWPKSAINYHFWPKNPPQYREANEEYATKLRSVADKLFEALS 693
            LQKE+GGKKGWVDHLFHIIWPKSA+NY+FWP NPP YREANEEYA  LR V DKLFEALS
Sbjct: 121  LQKELGGKKGWVDHLFHIIWPKSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFEALS 180

Query: 694  LGLGLERHAMKEAVGGDDLIYLMKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVQGL 873
            LG+GLE+H +K+A GGDDLIY++KINYYPPCPRPDLALGVVAHTDMS +TILVPNEVQGL
Sbjct: 181  LGIGLEKHELKKASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEVQGL 240

Query: 874  QVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGQYKSVYHRTTVNKDKTRMSWPVFLEPPP 1053
            QVHKDDHWYDVKYIPNALIIHIGDQIEIMSNG+YKSVYHRTTVNKDKTRMSWPVFLEPPP
Sbjct: 241  QVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPP 300

Query: 1054 ELLTGPFEKLVTDENPAKFKTKKYKDYVY 1140
            ELLTGP  KL+TDENPAKFKTKKYKDYVY
Sbjct: 301  ELLTGPISKLITDENPAKFKTKKYKDYVY 329


>sp|Q07512.1|FLS_PETHY RecName: Full=Flavonol synthase/flavanone 3-hydroxylase; Short=FLS
            gi|311658|emb|CAA80264.1| flavonol synthase [Petunia x
            hybrida]
          Length = 348

 Score =  546 bits (1407), Expect = e-153
 Identities = 256/328 (78%), Positives = 288/328 (87%), Gaps = 1/328 (0%)
 Frame = +1

Query: 160  MEVERVQAISKMSKCMDTIPSEYIRSENEQPAVTTMQGVVLEVPVIDLGSPDEENLVRLI 339
            MEV RVQAI+ +SKCMDTIPSEYIRSENEQPA TT+ GVVL+VPVIDL  PDE  +V+LI
Sbjct: 13   MEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHGVVLQVPVIDLRDPDENKMVKLI 72

Query: 340  ADASREWGIFQVVNHGIPDEAIAKLQKVGKEFFE-LPQEEKEVIAKPEGYQGVEGYGTKL 516
            ADAS+EWGIFQ++NHGIPDEAIA LQKVGKEFFE +PQEEKE+IAK  G   +EGYGT L
Sbjct: 73   ADASKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTPGSNDIEGYGTSL 132

Query: 517  QKEVGGKKGWVDHLFHIIWPKSAINYHFWPKNPPQYREANEEYATKLRSVADKLFEALSL 696
            QKEV GKKGWVDHLFH IWP SA+NY +WPKNPP YREANEEY  ++R V D++F++LSL
Sbjct: 133  QKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSLSL 192

Query: 697  GLGLERHAMKEAVGGDDLIYLMKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVQGLQ 876
            GLGLE H M EA GGD+++YL+KINYYPPCPRPDLALGVVAHTDMS +TILVPNEVQGLQ
Sbjct: 193  GLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQGLQ 252

Query: 877  VHKDDHWYDVKYIPNALIIHIGDQIEIMSNGQYKSVYHRTTVNKDKTRMSWPVFLEPPPE 1056
            V KD HWYDVKYIPNALI+HIGDQ+EI+SNG+YKSVYHRTTVNKDKTRMSWPVFLEPP E
Sbjct: 253  VFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPSE 312

Query: 1057 LLTGPFEKLVTDENPAKFKTKKYKDYVY 1140
               GP  KL+++ NP KFKTKKYKDYVY
Sbjct: 313  HEVGPIPKLLSEANPPKFKTKKYKDYVY 340


>gb|ADZ28516.1| flavonol synthase [Camellia nitidissima]
          Length = 336

 Score =  544 bits (1401), Expect = e-152
 Identities = 256/328 (78%), Positives = 292/328 (89%), Gaps = 1/328 (0%)
 Frame = +1

Query: 160  MEVERVQAISKMSKCMDTIPSEYIRSENEQPAVTTMQGVVLEVPVIDLGSP-DEENLVRL 336
            ME+ERVQ I+ +SKC+DTIP+EYIRSENEQPA+TT+QG VLEVPVIDL +  D++N+V++
Sbjct: 1    MELERVQVIASLSKCIDTIPAEYIRSENEQPAITTIQGKVLEVPVIDLSNDSDDQNIVQM 60

Query: 337  IADASREWGIFQVVNHGIPDEAIAKLQKVGKEFFELPQEEKEVIAKPEGYQGVEGYGTKL 516
            +A+A R+WGIFQVVNHGIPDE I  LQ+VGK+FFELPQEEKEV AKP   + VEGYGTKL
Sbjct: 61   VAEACRDWGIFQVVNHGIPDEVIGNLQRVGKDFFELPQEEKEVYAKPPESKSVEGYGTKL 120

Query: 517  QKEVGGKKGWVDHLFHIIWPKSAINYHFWPKNPPQYREANEEYATKLRSVADKLFEALSL 696
            QKEV GKKGWVDHLFH IWP SAINY FWPKNPP YREANE YA +LR+VADKLF  LSL
Sbjct: 121  QKEVEGKKGWVDHLFHKIWPPSAINYKFWPKNPPSYREANEVYAERLRAVADKLFRWLSL 180

Query: 697  GLGLERHAMKEAVGGDDLIYLMKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVQGLQ 876
            GLGLE H +KEAVGGDDLIYLMKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEV GLQ
Sbjct: 181  GLGLEGHEVKEAVGGDDLIYLMKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPGLQ 240

Query: 877  VHKDDHWYDVKYIPNALIIHIGDQIEIMSNGQYKSVYHRTTVNKDKTRMSWPVFLEPPPE 1056
            V KDDHWY+VKYIPNALI+HIGDQIEI+SNG+YK+V+HR+TVNK+ TRMSWPVF+EP PE
Sbjct: 241  VFKDDHWYEVKYIPNALIVHIGDQIEIVSNGKYKAVFHRSTVNKETTRMSWPVFIEPSPE 300

Query: 1057 LLTGPFEKLVTDENPAKFKTKKYKDYVY 1140
            +  GP  KL+ DENP K+KTKKY+DY+Y
Sbjct: 301  VEVGPHPKLIDDENPPKYKTKKYEDYMY 328


>gb|ACL98052.1| flavonol synthase [Camellia sinensis]
          Length = 336

 Score =  542 bits (1396), Expect = e-152
 Identities = 255/328 (77%), Positives = 292/328 (89%), Gaps = 1/328 (0%)
 Frame = +1

Query: 160  MEVERVQAISKMSKCMDTIPSEYIRSENEQPAVTTMQGVVLEVPVIDLGSP-DEENLVRL 336
            ME+ERVQ I+ +SKC+DTIP+EYIRSENEQPA+TT+QG VLEVPVIDL +  D++N+V+L
Sbjct: 1    MELERVQVIASLSKCIDTIPAEYIRSENEQPAITTIQGKVLEVPVIDLNNDSDDQNIVQL 60

Query: 337  IADASREWGIFQVVNHGIPDEAIAKLQKVGKEFFELPQEEKEVIAKPEGYQGVEGYGTKL 516
            +A+A R+WGIFQVVNHGIPDE I  LQ+VGK+FFELPQEEKEV AKP   + VEGYGTKL
Sbjct: 61   VAEACRDWGIFQVVNHGIPDEVIGNLQRVGKDFFELPQEEKEVYAKPPESRSVEGYGTKL 120

Query: 517  QKEVGGKKGWVDHLFHIIWPKSAINYHFWPKNPPQYREANEEYATKLRSVADKLFEALSL 696
            QKEV GKKGWVDHLFH IWP SAINY FWPKNPP YREANE YA +LR+VADKLF  LSL
Sbjct: 121  QKEVEGKKGWVDHLFHKIWPPSAINYKFWPKNPPSYREANEVYAERLRAVADKLFRWLSL 180

Query: 697  GLGLERHAMKEAVGGDDLIYLMKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVQGLQ 876
            GLGLE H +KEAVGGDDLIYLMKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEV GLQ
Sbjct: 181  GLGLEGHEVKEAVGGDDLIYLMKINYYPPCPRPDLALGVVAHTDMSTLTILVPNEVPGLQ 240

Query: 877  VHKDDHWYDVKYIPNALIIHIGDQIEIMSNGQYKSVYHRTTVNKDKTRMSWPVFLEPPPE 1056
            V KDDHWY+VKYIPNALI+HIGDQIEI+SNG+Y++V+HR+TVNK+ TRMSWPVF+EP PE
Sbjct: 241  VFKDDHWYEVKYIPNALIVHIGDQIEIVSNGKYRAVFHRSTVNKETTRMSWPVFIEPSPE 300

Query: 1057 LLTGPFEKLVTDENPAKFKTKKYKDYVY 1140
            +  GP  KL+ D+NP K+KTKKY+DY+Y
Sbjct: 301  VEVGPHPKLIDDKNPPKYKTKKYEDYMY 328


>gb|ABE28017.1| flavonol synthase [Nicotiana tabacum]
          Length = 346

 Score =  541 bits (1395), Expect = e-152
 Identities = 258/337 (76%), Positives = 293/337 (86%), Gaps = 1/337 (0%)
 Frame = +1

Query: 133  QTKKA*KRKMEVERVQAISKMSKCMDTIPSEYIRSENEQPAVTTMQGVVLEVPVIDLGSP 312
            Q + A    MEV RVQAI+ ++KCMDTIPSEYIRSENEQPA TT+ GV+L+VPVID+   
Sbjct: 5    QAQSATTLTMEVARVQAIASITKCMDTIPSEYIRSENEQPASTTLHGVLLQVPVIDI--- 61

Query: 313  DEENLVRLIADASREWGIFQVVNHGIPDEAIAKLQKVGKEFFEL-PQEEKEVIAKPEGYQ 489
            D++N+V+LI+DAS+EWGIFQV+NHGIPDE IA LQKVGKEFFE+ PQEEKEVIAK  G Q
Sbjct: 62   DDKNVVKLISDASKEWGIFQVINHGIPDEVIANLQKVGKEFFEVVPQEEKEVIAKTPGSQ 121

Query: 490  GVEGYGTKLQKEVGGKKGWVDHLFHIIWPKSAINYHFWPKNPPQYREANEEYATKLRSVA 669
             +EGYGT LQKE+ GK+GWVDHLFH IWP SAINY +WPKNPP YREANEEYA +LR VA
Sbjct: 122  NIEGYGTSLQKELEGKRGWVDHLFHKIWPPSAINYRYWPKNPPSYREANEEYAKRLREVA 181

Query: 670  DKLFEALSLGLGLERHAMKEAVGGDDLIYLMKINYYPPCPRPDLALGVVAHTDMSTLTIL 849
            +K+F++LSLGLGLE H M EA GG++++YL+KINYYPPCPRPDLALGVVAHTDMS +TIL
Sbjct: 182  EKIFKSLSLGLGLEAHEMMEAAGGEEIVYLLKINYYPPCPRPDLALGVVAHTDMSHITIL 241

Query: 850  VPNEVQGLQVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGQYKSVYHRTTVNKDKTRMSW 1029
            VPNEVQGLQV KDDHWYDVKYIPNALIIHIGDQ+EI+SNG+YKSVYHRTTV KDKTRMSW
Sbjct: 242  VPNEVQGLQVFKDDHWYDVKYIPNALIIHIGDQVEILSNGKYKSVYHRTTVTKDKTRMSW 301

Query: 1030 PVFLEPPPELLTGPFEKLVTDENPAKFKTKKYKDYVY 1140
            PVFLEPP E   GP  KLV + NP KFKTKKYKDYVY
Sbjct: 302  PVFLEPPSEHEVGPIPKLVNEANPPKFKTKKYKDYVY 338