BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003350
         (1785 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002535132.1| Polygalacturonase precursor, putative [Ricin...   687   0.0  
ref|XP_002273855.2| PREDICTED: probable polygalacturonase-like [...   682   0.0  
ref|XP_002301554.1| predicted protein [Populus trichocarpa] gi|2...   667   0.0  
ref|XP_003626072.1| Polygalacturonase [Medicago truncatula] gi|3...   665   0.0  
ref|XP_002320934.1| predicted protein [Populus trichocarpa] gi|2...   657   0.0  

>ref|XP_002535132.1| Polygalacturonase precursor, putative [Ricinus communis]
            gi|223523955|gb|EEF27252.1| Polygalacturonase precursor,
            putative [Ricinus communis]
          Length = 476

 Score =  687 bits (1773), Expect = 0.0
 Identities = 319/447 (71%), Positives = 385/447 (86%), Gaps = 2/447 (0%)
 Frame = +1

Query: 313  FGEAQWVMGGGSLCRESSSVIVRPHSVSITEFGAVGDGLTLNTKAFENAIFYLNSFADKG 492
            F +  W + G S C+ ++S  +RPHSV+ITEFGAVGDG+TLNTKAF+NAIFYLNSF+DKG
Sbjct: 27   FSDVTWHIRGSSHCKRANSADIRPHSVTITEFGAVGDGVTLNTKAFQNAIFYLNSFSDKG 86

Query: 493  GAQLFIPEGRWLTGSFTLISHLTLRLDKDAEILGSTNSDDWPVIDPLPSYGRGRELPGER 672
            GA+LF+P GRWLTGSF LISHLTL LDK+A ILGSTNSDDWPV+D LPSYGRGRELPG R
Sbjct: 87   GAKLFVPAGRWLTGSFDLISHLTLWLDKNAVILGSTNSDDWPVVDALPSYGRGRELPGGR 146

Query: 673  HRSLIYGRNLTDVIITGDNGTIDGQGSVWWNWFRSETLNYTRPHMLELIDSKEVIISNLT 852
            HRSLIYGRNLTDV+ITGDNGTIDGQGS+WW WF++E+LNYTRPH++EL++S  ++ISNLT
Sbjct: 147  HRSLIYGRNLTDVVITGDNGTIDGQGSIWWTWFKTESLNYTRPHLVELMNSSGIVISNLT 206

Query: 853  FRNSPFWNIHPVYCSQVIIQNVTIIAPLDSPNTDGIDPDSSSNVCIEDCYISTGDDVIAI 1032
            F NSPFW IHPVYCS+VI+QNVTI APLDSPNTDGIDPDSS +VCIEDC+ISTGDD+IAI
Sbjct: 207  FINSPFWTIHPVYCSKVIVQNVTIRAPLDSPNTDGIDPDSSDDVCIEDCFISTGDDLIAI 266

Query: 1033 KSGWDEYGISYARPSTNITIHRLVGQTTSSSGIAIGSEMSGGVSEVYVKDVHFFSSNRGI 1212
            KSGWDEYGI Y RP  NITI RLVGQT SS+GIAIGSEMSGGVSEV+ +++ F++SN GI
Sbjct: 267  KSGWDEYGILYGRPCRNITIRRLVGQTRSSAGIAIGSEMSGGVSEVHAENILFYNSNTGI 326

Query: 1213 SIKTSRGRGGYVRNIFITGVVLSNLKVAIKFNGNYGEHPDEHYDPNALPLIEKISIHNVT 1392
             IKTS GRGGYVRNI+++ V L+++ +AI+F GNYGEHPDEHYDP ALP+IE+I+I +V 
Sbjct: 327  RIKTSPGRGGYVRNIYVSNVTLNDVNIAIRFTGNYGEHPDEHYDPKALPIIERITIEHVM 386

Query: 1393 GENIKVAGLLEGIEEDTFKRICLSDITLDVRSKCPWNCSNVEGFSNKVSPDICKLLKRKI 1572
            G+NIK AG+L+GIE D+F  ICLS+I+L+V SK PWNCS ++G+S  VSP+IC+ L+  I
Sbjct: 387  GDNIKYAGILDGIEADSFVNICLSNISLNVTSKFPWNCSYIQGYSESVSPEICEPLRESI 446

Query: 1573 TSE--SKCYHVSNHLRSISPQNRGTWL 1647
              +  S CYH+SN+L +   QNRGTWL
Sbjct: 447  PPDHYSNCYHLSNYLLNSRNQNRGTWL 473


>ref|XP_002273855.2| PREDICTED: probable polygalacturonase-like [Vitis vinifera]
            gi|297744085|emb|CBI37055.3| unnamed protein product
            [Vitis vinifera]
          Length = 466

 Score =  682 bits (1759), Expect = 0.0
 Identities = 325/447 (72%), Positives = 378/447 (84%), Gaps = 2/447 (0%)
 Frame = +1

Query: 313  FGEAQWVMGGGSLCRESSSVIVRPHSVSITEFGAVGDGLTLNTKAFENAIFYLNSFADKG 492
            F EA W + G   C ++SS ++RPHSV+ITEFGAVGDG+TLNTKAF+NAIFYLNSFADKG
Sbjct: 17   FSEAPWAVWGSPHCDQTSSGVIRPHSVAITEFGAVGDGVTLNTKAFQNAIFYLNSFADKG 76

Query: 493  GAQLFIPEGRWLTGSFTLISHLTLRLDKDAEILGSTNSDDWPVIDPLPSYGRGRELPGER 672
            GAQLF+P GRWLTGSF LISHLTL LDKDA ILGS NS+DWPVIDPLPSYGRGRELPG R
Sbjct: 77   GAQLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSMNSNDWPVIDPLPSYGRGRELPGRR 136

Query: 673  HRSLIYGRNLTDVIITGDNGTIDGQGSVWWNWFRSETLNYTRPHMLELIDSKEVIISNLT 852
            HRSLIYG NLTDVI+TGDNGTIDGQGS+WWNWF+ +TLNYTRPH++E I+S  V+ISN+T
Sbjct: 137  HRSLIYGCNLTDVIVTGDNGTIDGQGSIWWNWFQKKTLNYTRPHLVEFINSTGVVISNVT 196

Query: 853  FRNSPFWNIHPVYCSQVIIQNVTIIAPLDSPNTDGIDPDSSSNVCIEDCYISTGDDVIAI 1032
            F NSPFW IHPVYCSQVIIQNVTI+APLDSPNTDGIDPDSS++VCIEDCYISTGDD+IAI
Sbjct: 197  FLNSPFWTIHPVYCSQVIIQNVTILAPLDSPNTDGIDPDSSNDVCIEDCYISTGDDLIAI 256

Query: 1033 KSGWDEYGISYARPSTNITIHRLVGQTTSSSGIAIGSEMSGGVSEVYVKDVHFFSSNRGI 1212
            KSGWDEYGISYARPSTNI I RLVG+T SS+GIAIGSEMSGGVSEV+ + + FF+S  GI
Sbjct: 257  KSGWDEYGISYARPSTNIIIRRLVGKTNSSAGIAIGSEMSGGVSEVHAESLQFFNSKTGI 316

Query: 1213 SIKTSRGRGGYVRNIFITGVVLSNLKVAIKFNGNYGEHPDEHYDPNALPLIEKISIHNVT 1392
             IKTS GRGGYVRNI+I+ + L ++K+AI+F G YGEHPDE YDP ALP+IE I++ +V 
Sbjct: 317  RIKTSPGRGGYVRNIYISDMNLVDVKIAIRFTGQYGEHPDEFYDPTALPIIENITVKDVM 376

Query: 1393 GENIKVAGLLEGIEEDTFKRICLSDITLDVRSKCPWNCSNVEGFSNKVSPDICKLLKRKI 1572
            GENIK AGLLEGIE D F  ICLS+ITL+V S+ PWNCS + G+S+ VSP+ C+ L  +I
Sbjct: 377  GENIKFAGLLEGIEGDNFVNICLSNITLNVTSESPWNCSYIHGYSDLVSPEACEPLGERI 436

Query: 1573 TSES--KCYHVSNHLRSISPQNRGTWL 1647
                   CYH+  HL S+S + R +WL
Sbjct: 437  YPGHVLDCYHLPTHLWSMSNRKRSSWL 463


>ref|XP_002301554.1| predicted protein [Populus trichocarpa] gi|222843280|gb|EEE80827.1|
            predicted protein [Populus trichocarpa]
          Length = 458

 Score =  667 bits (1721), Expect = 0.0
 Identities = 312/429 (72%), Positives = 371/429 (86%), Gaps = 2/429 (0%)
 Frame = +1

Query: 313  FGEAQWVMGGGSLCRESSSVIVRPHSVSITEFGAVGDGLTLNTKAFENAIFYLNSFADKG 492
            FG+A W +    LC + +  ++RPHSV+ITEFGAVGDG+TLNTKAF+NAIFYLNSFADKG
Sbjct: 17   FGDAPWAIKCSLLCEQGNLAVIRPHSVTITEFGAVGDGVTLNTKAFQNAIFYLNSFADKG 76

Query: 493  GAQLFIPEGRWLTGSFTLISHLTLRLDKDAEILGSTNSDDWPVIDPLPSYGRGRELPGER 672
            GA+LF+P GRWLTGSF LISHLTL LDKDA ILGSTNSDDWPVIDPLPSYGRGRELPG R
Sbjct: 77   GAKLFVPTGRWLTGSFDLISHLTLWLDKDAVILGSTNSDDWPVIDPLPSYGRGRELPGRR 136

Query: 673  HRSLIYGRNLTDVIITGDNGTIDGQGSVWWNWFRSETLNYTRPHMLELIDSKEVIISNLT 852
            H+SLIYGRNLTDVIITGDNGTIDGQGS+WW+WFR++TLNYTRPH++E +++  V+ISNLT
Sbjct: 137  HKSLIYGRNLTDVIITGDNGTIDGQGSIWWDWFRNQTLNYTRPHLVEFMNTTGVVISNLT 196

Query: 853  FRNSPFWNIHPVYCSQVIIQNVTIIAPLDSPNTDGIDPDSSSNVCIEDCYISTGDDVIAI 1032
            F NSPFW IHPVYCSQVI+QNVTI+APLDSPNTDGIDPDSS +VC+EDCY+STGDD+IAI
Sbjct: 197  FINSPFWTIHPVYCSQVIVQNVTILAPLDSPNTDGIDPDSSDDVCVEDCYVSTGDDIIAI 256

Query: 1033 KSGWDEYGISYARPSTNITIHRLVGQTTSSSGIAIGSEMSGGVSEVYVKDVHFFSSNRGI 1212
            KSGWDEYG+SY RPS NITI RLVGQTT S+GIAIGSEMSGGVSEV+ +++ F++S  GI
Sbjct: 257  KSGWDEYGMSYGRPSKNITIRRLVGQTT-SAGIAIGSEMSGGVSEVHAENLRFYNSTTGI 315

Query: 1213 SIKTSRGRGGYVRNIFITGVVLSNLKVAIKFNGNYGEHPDEHYDPNALPLIEKISIHNVT 1392
             IKTS GRGGYVRNI+I+ + L+++K AI F G YGEHPDE+YDP ALPLIE+I++ +V 
Sbjct: 316  RIKTSPGRGGYVRNIYISNMSLTDVKTAISFTGRYGEHPDEYYDPTALPLIERITVEDVA 375

Query: 1393 GENIKVAGLLEGIEEDTFKRICLSDITLDVRSKCPWNCSNVEGFSNKVSPDICKLLKRKI 1572
            G+N+K AGLLEGIE DTF  ICL +I L V SK PWNCS ++G+S+ VSP+IC+ L+ +I
Sbjct: 376  GQNVKYAGLLEGIEGDTFLDICLLNINLSVTSKSPWNCSYIQGYSDTVSPEICEPLRERI 435

Query: 1573 TSE--SKCY 1593
              +  S CY
Sbjct: 436  FPDHYSDCY 444


>ref|XP_003626072.1| Polygalacturonase [Medicago truncatula] gi|355501087|gb|AES82290.1|
            Polygalacturonase [Medicago truncatula]
          Length = 466

 Score =  665 bits (1715), Expect = 0.0
 Identities = 313/441 (70%), Positives = 377/441 (85%), Gaps = 2/441 (0%)
 Frame = +1

Query: 322  AQWVMGGGSLCRESSSVIVRPHSVSITEFGAVGDGLTLNTKAFENAIFYLNSFADKGGAQ 501
            + W +   +LC+E++   VRPHSVSITEFGAVGDG+TLNTKAF+NAIFYLNSF+DKGGA+
Sbjct: 20   SSWKVWSSTLCKETNLDKVRPHSVSITEFGAVGDGITLNTKAFQNAIFYLNSFSDKGGAK 79

Query: 502  LFIPEGRWLTGSFTLISHLTLRLDKDAEILGSTNSDDWPVIDPLPSYGRGRELPGERHRS 681
            LF+P GRWLTGSF LISHLTL LDKDA ILGSTNS+DWPV+DPLPSYGRGRELPG RHRS
Sbjct: 80   LFVPAGRWLTGSFDLISHLTLWLDKDAIILGSTNSEDWPVVDPLPSYGRGRELPGGRHRS 139

Query: 682  LIYGRNLTDVIITGDNGTIDGQGSVWWNWFRSETLNYTRPHMLELIDSKEVIISNLTFRN 861
            LIYG NLTDVIITG+ G IDGQGS+WW+ FR++TL+YTRPH++EL++S  V+ISNLTF N
Sbjct: 140  LIYGCNLTDVIITGNEGIIDGQGSIWWSMFRNKTLDYTRPHLVELMNSTRVLISNLTFLN 199

Query: 862  SPFWNIHPVYCSQVIIQNVTIIAPLDSPNTDGIDPDSSSNVCIEDCYISTGDDVIAIKSG 1041
            SPFW IHPVYCSQV +QNVTI+APLDSPNTDGIDPDSS +VCIEDCYISTGDD+IAIKSG
Sbjct: 200  SPFWTIHPVYCSQVTVQNVTILAPLDSPNTDGIDPDSSDDVCIEDCYISTGDDLIAIKSG 259

Query: 1042 WDEYGISYARPSTNITIHRLVGQTTSSSGIAIGSEMSGGVSEVYVKDVHFFSSNRGISIK 1221
            WDEYGI++ RPSTNI IHRLVG+T +S+GIAIGSEMSGGVS+V  +D+HF+ S   I IK
Sbjct: 260  WDEYGIAFGRPSTNIVIHRLVGKTHTSAGIAIGSEMSGGVSDVRAEDIHFYDSYTAIRIK 319

Query: 1222 TSRGRGGYVRNIFITGVVLSNLKVAIKFNGNYGEHPDEHYDPNALPLIEKISIHNVTGEN 1401
            TS GRGGYVRNI++T + L+N+ +AI+F G+YG+HPD+ YDPNALP+IEKI+I +V GEN
Sbjct: 320  TSPGRGGYVRNIYVTNMTLANVDIAIRFTGSYGDHPDDAYDPNALPVIEKITIEDVIGEN 379

Query: 1402 IKVAGLLEGIEEDTFKRICLSDITLDVRSKCPWNCSNVEGFSNKVSPDICKLLKRKITSE 1581
            I  AGL+EGIE D F  ICLS+ITL+V S  PWNCSN+ G+S+ V P+ C+ LK +I  +
Sbjct: 380  ITKAGLIEGIEGDNFVNICLSNITLNVSSNYPWNCSNIRGYSDMVFPEACEPLKERIFPD 439

Query: 1582 --SKCYHVSNHLRSISPQNRG 1638
              S CY++SNH +S + QNRG
Sbjct: 440  HCSDCYYLSNHRQSSNNQNRG 460


>ref|XP_002320934.1| predicted protein [Populus trichocarpa] gi|222861707|gb|EEE99249.1|
            predicted protein [Populus trichocarpa]
          Length = 427

 Score =  657 bits (1696), Expect = 0.0
 Identities = 310/417 (74%), Positives = 365/417 (87%)
 Frame = +1

Query: 313  FGEAQWVMGGGSLCRESSSVIVRPHSVSITEFGAVGDGLTLNTKAFENAIFYLNSFADKG 492
            F +A W + G S C+  +  ++RPHSV+ITEFGAVGDG+TLNTKAF+NAIFYLNSFADKG
Sbjct: 10   FIDAPWAIRGNSHCQWGNPAVIRPHSVAITEFGAVGDGVTLNTKAFQNAIFYLNSFADKG 69

Query: 493  GAQLFIPEGRWLTGSFTLISHLTLRLDKDAEILGSTNSDDWPVIDPLPSYGRGRELPGER 672
            GA+LF+P G+WLTGSF LISHLTL LDKDA ILGSTNSDDWPVID LPSYGRGRELPG R
Sbjct: 70   GAKLFVPAGQWLTGSFDLISHLTLWLDKDAIILGSTNSDDWPVIDSLPSYGRGRELPGRR 129

Query: 673  HRSLIYGRNLTDVIITGDNGTIDGQGSVWWNWFRSETLNYTRPHMLELIDSKEVIISNLT 852
            H+SLIYGRNLTDVIITGDNGTIDGQGS+WWNWFR+ETL+YTRPH++EL+++  V+ISNLT
Sbjct: 130  HKSLIYGRNLTDVIITGDNGTIDGQGSIWWNWFRNETLDYTRPHLVELMNTTGVVISNLT 189

Query: 853  FRNSPFWNIHPVYCSQVIIQNVTIIAPLDSPNTDGIDPDSSSNVCIEDCYISTGDDVIAI 1032
            F NSPFW IHPVYCSQVI+QNVTI+APLDSPNTDGIDPDSS +VCIEDCYISTGDD+IAI
Sbjct: 190  FLNSPFWTIHPVYCSQVIVQNVTILAPLDSPNTDGIDPDSSDDVCIEDCYISTGDDIIAI 249

Query: 1033 KSGWDEYGISYARPSTNITIHRLVGQTTSSSGIAIGSEMSGGVSEVYVKDVHFFSSNRGI 1212
            KSGWDEYG SYARPS NITI  LVGQTT S+GIAIGSEMSGGVSEV+ +++ F++S  GI
Sbjct: 250  KSGWDEYGTSYARPSKNITIRGLVGQTT-SAGIAIGSEMSGGVSEVHAENLTFYNSTTGI 308

Query: 1213 SIKTSRGRGGYVRNIFITGVVLSNLKVAIKFNGNYGEHPDEHYDPNALPLIEKISIHNVT 1392
             IKT+ GRGGYVRNI+I+ + L+++K AI+F G YG+HPDE YDP ALPLIE+I+I +VT
Sbjct: 309  RIKTAPGRGGYVRNIYISNMSLTDVKTAIRFTGQYGDHPDESYDPKALPLIERITIDDVT 368

Query: 1393 GENIKVAGLLEGIEEDTFKRICLSDITLDVRSKCPWNCSNVEGFSNKVSPDICKLLK 1563
            G+N+K AGLLEG+E DTF  ICLS+I L V SK PWNCS ++G+S  VSP+IC+ L+
Sbjct: 369  GQNVKYAGLLEGLEGDTFLDICLSNINLSVTSKSPWNCSYIQGYSEAVSPEICEPLR 425