BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003341
         (1382 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003546241.1| PREDICTED: cysteine desulfurase 2, chloropla...   693   0.0  
ref|XP_002523229.1| cysteine desulfurylase, putative [Ricinus co...   691   0.0  
ref|XP_002267920.1| PREDICTED: cysteine desulfurase 2, chloropla...   690   0.0  
ref|XP_002892460.1| hypothetical protein ARALYDRAFT_470910 [Arab...   679   0.0  
ref|NP_172325.2| cysteine desulfurase 2 [Arabidopsis thaliana] g...   678   0.0  

>ref|XP_003546241.1| PREDICTED: cysteine desulfurase 2, chloroplastic-like [Glycine max]
          Length = 468

 Score =  693 bits (1789), Expect = 0.0
 Identities = 334/419 (79%), Positives = 381/419 (90%)
 Frame = -2

Query: 1315 VNDRQSDPKVEPLPSLGLATRPDFPILHQEINGSKLVYLDNAATSQKPTAVLNALQNYYE 1136
            VN+  ++P V  L SLG +TRP FPILHQE+NGSKLVYLDNAATSQKPTAVL ALQNYYE
Sbjct: 44   VNEAVAEPIVGFL-SLGHSTRPHFPILHQEVNGSKLVYLDNAATSQKPTAVLKALQNYYE 102

Query: 1135 AYNSNVHRGVHFLSAKATDAYELARKKVATFINAPDSRDIVFTRNATEAINLVAYSWGLL 956
            AYNSNVHRG+HFLSA+ATD YE AR+KVA+FINA DSR+IVFT+NA+EAINLVAYSWGL 
Sbjct: 103  AYNSNVHRGIHFLSARATDEYESARRKVASFINAIDSREIVFTKNASEAINLVAYSWGLS 162

Query: 955  NLKPDDEVVLTIAEHHSAVVPWQLVAQKTGAVLKFVSLTDDEVPDLVELRGLLSVKTKLV 776
            NLKPDDE++LT+AEHHSA+VPWQ+VAQKTGAVLKFV L  DE+PD+ +L+ +LS KTK+V
Sbjct: 163  NLKPDDEIILTVAEHHSAIVPWQIVAQKTGAVLKFVDLNQDEIPDIDKLKEMLSRKTKIV 222

Query: 775  VLHHVSNVLASVLPIEEIVLWAHDVGAKVLVDACQSVPHMVVDVKSLNADFLVASSHKMC 596
            V+HHVSNVLASVLPI++I  WAHDVGAKVLVDACQSVPHM++DV+SLN DFLVASSHKMC
Sbjct: 223  VVHHVSNVLASVLPIQDIAQWAHDVGAKVLVDACQSVPHMMIDVQSLNVDFLVASSHKMC 282

Query: 595  GPTGIGFLYGRSELLAAMPPFLGGGEMIADVFLDHSTYAEPPSRFEAGTPAIGEAVGFGA 416
            GPTGIGFLYG+ +LL++MPPFLGGGEMI+DV+LDHSTYAEPPSRFEAGTPAIGEA+G GA
Sbjct: 283  GPTGIGFLYGKIDLLSSMPPFLGGGEMISDVYLDHSTYAEPPSRFEAGTPAIGEAIGLGA 342

Query: 415  AIDYLSSIGMQKIHDYEVELANYLYGSLKEVPNVRIYGPAPSKHVTRAALCCFNVEDIHP 236
            AIDYLS IGMQ IHDYEVEL  YLY  L  VPN+RIYGPAPS+ V RAALC FNVE++HP
Sbjct: 343  AIDYLSGIGMQTIHDYEVELGRYLYERLLSVPNIRIYGPAPSEKVERAALCSFNVENLHP 402

Query: 235  TDIATFLDQQHGVAIRSGHHCAQPLHRYLGINASARASLYFYNTKQDVDDFIDALKDTI 59
            TD+AT LDQQHGVAIRSGHHCAQPLHRYLG+++SARASLYFYNTK+DVD+FI AL DT+
Sbjct: 403  TDLATLLDQQHGVAIRSGHHCAQPLHRYLGVSSSARASLYFYNTKEDVDNFIHALNDTV 461


>ref|XP_002523229.1| cysteine desulfurylase, putative [Ricinus communis]
            gi|223537525|gb|EEF39150.1| cysteine desulfurylase,
            putative [Ricinus communis]
          Length = 469

 Score =  691 bits (1783), Expect = 0.0
 Identities = 335/438 (76%), Positives = 382/438 (87%), Gaps = 4/438 (0%)
 Frame = -2

Query: 1360 HNFSSRARTFSA----SLIVNDRQSDPKVEPLPSLGLATRPDFPILHQEINGSKLVYLDN 1193
            H+   R   FS+    +L     + D  +    SLG  TRPDFPILHQE+NGSKLVYLDN
Sbjct: 25   HSSPIRLAKFSSFAIPALATTGTEGDQALHASLSLGHITRPDFPILHQEVNGSKLVYLDN 84

Query: 1192 AATSQKPTAVLNALQNYYEAYNSNVHRGVHFLSAKATDAYELARKKVATFINAPDSRDIV 1013
            AATSQKP +VL A+QNYYE+YNSNVHRG+H+LSAKATD YELARKKVA FINA DSR+IV
Sbjct: 85   AATSQKPNSVLKAIQNYYESYNSNVHRGIHYLSAKATDEYELARKKVAAFINASDSREIV 144

Query: 1012 FTRNATEAINLVAYSWGLLNLKPDDEVVLTIAEHHSAVVPWQLVAQKTGAVLKFVSLTDD 833
            FTRNATEAINLVAYSWG  NLKP DEV+LT+AEHHS +VPWQLVA +TGAVL+FV L ++
Sbjct: 145  FTRNATEAINLVAYSWGFTNLKPGDEVILTLAEHHSNIVPWQLVANRTGAVLRFVDLNEN 204

Query: 832  EVPDLVELRGLLSVKTKLVVLHHVSNVLASVLPIEEIVLWAHDVGAKVLVDACQSVPHMV 653
            EVPD+++L+  +S KTKLVV+HH+SNVLAS LP+E+I+ WAH  GAKVL+DACQSVPHMV
Sbjct: 205  EVPDVLKLKETISEKTKLVVVHHISNVLASFLPVEDIIHWAHGAGAKVLIDACQSVPHMV 264

Query: 652  VDVKSLNADFLVASSHKMCGPTGIGFLYGRSELLAAMPPFLGGGEMIADVFLDHSTYAEP 473
            VDV+ L+ADFLVASSHKMCGPTGIGFLYG+S++L+ MPPFLGGGEMIADVFLDHSTYA+P
Sbjct: 265  VDVQGLDADFLVASSHKMCGPTGIGFLYGKSDILSTMPPFLGGGEMIADVFLDHSTYADP 324

Query: 472  PSRFEAGTPAIGEAVGFGAAIDYLSSIGMQKIHDYEVELANYLYGSLKEVPNVRIYGPAP 293
            PSRFEAGTPAIGEA+G GAAIDYL  IGMQKIHDYE+ELANYLY SL  VP+VRIYGP P
Sbjct: 325  PSRFEAGTPAIGEAIGLGAAIDYLLGIGMQKIHDYEMELANYLYESLSSVPSVRIYGPKP 384

Query: 292  SKHVTRAALCCFNVEDIHPTDIATFLDQQHGVAIRSGHHCAQPLHRYLGINASARASLYF 113
            S++V RAALC FNVE+IHPTDIATFLDQQHGVAIRSGHHCAQPLHR+LG+NASARASL+F
Sbjct: 385  SENVHRAALCSFNVENIHPTDIATFLDQQHGVAIRSGHHCAQPLHRHLGVNASARASLHF 444

Query: 112  YNTKQDVDDFIDALKDTI 59
            YNTK+DVDDFI AL DTI
Sbjct: 445  YNTKEDVDDFIQALNDTI 462


>ref|XP_002267920.1| PREDICTED: cysteine desulfurase 2, chloroplastic [Vitis vinifera]
            gi|296083920|emb|CBI24308.3| unnamed protein product
            [Vitis vinifera]
          Length = 463

 Score =  690 bits (1780), Expect = 0.0
 Identities = 334/405 (82%), Positives = 374/405 (92%)
 Frame = -2

Query: 1273 SLGLATRPDFPILHQEINGSKLVYLDNAATSQKPTAVLNALQNYYEAYNSNVHRGVHFLS 1094
            SLG  TRPDFPILHQE+NGSKLVYLDNAATSQKPTAVL ALQNYYEAYNSNVHRG+HFLS
Sbjct: 52   SLGHLTRPDFPILHQEVNGSKLVYLDNAATSQKPTAVLKALQNYYEAYNSNVHRGIHFLS 111

Query: 1093 AKATDAYELARKKVATFINAPDSRDIVFTRNATEAINLVAYSWGLLNLKPDDEVVLTIAE 914
            AKATD YE AR+KVA FINA +  +I+FTRNATEAINLVAYSWGL NLKP+DE+VLT+AE
Sbjct: 112  AKATDEYESARRKVAAFINASEPGEIIFTRNATEAINLVAYSWGLSNLKPEDEIVLTVAE 171

Query: 913  HHSAVVPWQLVAQKTGAVLKFVSLTDDEVPDLVELRGLLSVKTKLVVLHHVSNVLASVLP 734
            HHSA+VPWQLVAQKTGA+LKFV+LT+DEVPD+ +L+ ++S KTKL+V+HH+SNVLAS LP
Sbjct: 172  HHSAIVPWQLVAQKTGAILKFVNLTEDEVPDVEKLKEMISRKTKLLVVHHISNVLASALP 231

Query: 733  IEEIVLWAHDVGAKVLVDACQSVPHMVVDVKSLNADFLVASSHKMCGPTGIGFLYGRSEL 554
            I +IV WAH VGAKVLVDACQSVPHM VDV+ L+ADFLVASSHKMCGPTGIGFLYG+S+L
Sbjct: 232  IYDIVHWAHAVGAKVLVDACQSVPHMAVDVQRLDADFLVASSHKMCGPTGIGFLYGKSDL 291

Query: 553  LAAMPPFLGGGEMIADVFLDHSTYAEPPSRFEAGTPAIGEAVGFGAAIDYLSSIGMQKIH 374
            L AMPPFLGGGEMI+DVFLDHST+AEPPSRFEAGTPAIGEA+G GAAIDYLS+IGMQKIH
Sbjct: 292  LFAMPPFLGGGEMISDVFLDHSTFAEPPSRFEAGTPAIGEAIGLGAAIDYLSAIGMQKIH 351

Query: 373  DYEVELANYLYGSLKEVPNVRIYGPAPSKHVTRAALCCFNVEDIHPTDIATFLDQQHGVA 194
            DYEV+LANYLY SL+ VP V +YGPAPS+ V RAALC FNVE+IHPTDIATFLDQQHGVA
Sbjct: 352  DYEVDLANYLYESLRSVPGVHVYGPAPSQTVFRAALCSFNVENIHPTDIATFLDQQHGVA 411

Query: 193  IRSGHHCAQPLHRYLGINASARASLYFYNTKQDVDDFIDALKDTI 59
            IRSGHHCAQPLHR+LG++ASARASL+FYNTK+DVD FI AL DTI
Sbjct: 412  IRSGHHCAQPLHRHLGVSASARASLHFYNTKEDVDAFIHALVDTI 456


>ref|XP_002892460.1| hypothetical protein ARALYDRAFT_470910 [Arabidopsis lyrata subsp.
            lyrata] gi|297338302|gb|EFH68719.1| hypothetical protein
            ARALYDRAFT_470910 [Arabidopsis lyrata subsp. lyrata]
          Length = 466

 Score =  679 bits (1752), Expect = 0.0
 Identities = 334/437 (76%), Positives = 374/437 (85%), Gaps = 3/437 (0%)
 Frame = -2

Query: 1360 HNFSSRARTFSASLIVNDRQSDPKVEP---LPSLGLATRPDFPILHQEINGSKLVYLDNA 1190
            H   SR R  S+  + +   S   +       SLG   R DF ILHQ +NGSKLVYLD+A
Sbjct: 23   HRSFSRVRRSSSLSVCSAASSSATISTDSESVSLGHRVRNDFRILHQVVNGSKLVYLDSA 82

Query: 1189 ATSQKPTAVLNALQNYYEAYNSNVHRGVHFLSAKATDAYELARKKVATFINAPDSRDIVF 1010
            ATSQKP AVL+ALQNYYE YNSNVHRG+H+LSAKATD +ELARKKVA FINA DSR+IVF
Sbjct: 83   ATSQKPAAVLDALQNYYEFYNSNVHRGIHYLSAKATDEFELARKKVARFINASDSREIVF 142

Query: 1009 TRNATEAINLVAYSWGLLNLKPDDEVVLTIAEHHSAVVPWQLVAQKTGAVLKFVSLTDDE 830
            TRNATEAINLVAYSWGL NLKP DEV+LT+AEHHS +VPWQ+V+QKTGAVLKFV+L DDE
Sbjct: 143  TRNATEAINLVAYSWGLSNLKPGDEVILTVAEHHSCIVPWQIVSQKTGAVLKFVTLNDDE 202

Query: 829  VPDLVELRGLLSVKTKLVVLHHVSNVLASVLPIEEIVLWAHDVGAKVLVDACQSVPHMVV 650
            VPD+ +LR ++S KTKLV +HHVSNVLAS LPIEEIV+WAHDVGAKVLVDACQSVPHMVV
Sbjct: 203  VPDIEKLREMISPKTKLVAVHHVSNVLASSLPIEEIVVWAHDVGAKVLVDACQSVPHMVV 262

Query: 649  DVKSLNADFLVASSHKMCGPTGIGFLYGRSELLAAMPPFLGGGEMIADVFLDHSTYAEPP 470
            DV+ LNADFLVASSHKMCGPTGIGFLYG+S+LL +MPPFLGGGEMI+DVFLDHSTYAEPP
Sbjct: 263  DVQKLNADFLVASSHKMCGPTGIGFLYGKSDLLHSMPPFLGGGEMISDVFLDHSTYAEPP 322

Query: 469  SRFEAGTPAIGEAVGFGAAIDYLSSIGMQKIHDYEVELANYLYGSLKEVPNVRIYGPAPS 290
            SRFEAGTPAIGEA+  GAA+DYLS IGM KIH+YEVEL  YLY  L  +P+VRIYGP PS
Sbjct: 323  SRFEAGTPAIGEAIALGAAVDYLSGIGMPKIHEYEVELGKYLYEKLSSLPDVRIYGPRPS 382

Query: 289  KHVTRAALCCFNVEDIHPTDIATFLDQQHGVAIRSGHHCAQPLHRYLGINASARASLYFY 110
            + V R ALC FNVE +HPTD+ATFLDQQHGVAIRSGHHCAQPLHRYLG+NASARASLYFY
Sbjct: 383  ESVHRGALCSFNVEGLHPTDLATFLDQQHGVAIRSGHHCAQPLHRYLGVNASARASLYFY 442

Query: 109  NTKQDVDDFIDALKDTI 59
            NTK+D+D FI AL DT+
Sbjct: 443  NTKEDIDSFIVALADTV 459


>ref|NP_172325.2| cysteine desulfurase 2 [Arabidopsis thaliana]
            gi|75249321|sp|Q93WX6.1|NFS2_ARATH RecName: Full=Cysteine
            desulfurase 2, chloroplastic; AltName: Full=NIFS-like
            protein; AltName: Full=Plastid sufS-like protein;
            AltName: Full=Protein AtCpNifS; AltName:
            Full=Selenocysteine lyase; Flags: Precursor
            gi|16152176|gb|AAL14994.1|AF419347_1 NIFS-like protein
            CpNifsp precursor [Arabidopsis thaliana]
            gi|20453112|gb|AAM19798.1| At1g08490/T27G7_14
            [Arabidopsis thaliana] gi|23506185|gb|AAN31104.1|
            At1g08490/T27G7_14 [Arabidopsis thaliana]
            gi|27085243|gb|AAL79956.1| cysteine desulfurase
            [Arabidopsis thaliana] gi|332190177|gb|AEE28298.1|
            cysteine desulfurase 2 [Arabidopsis thaliana]
          Length = 463

 Score =  678 bits (1750), Expect = 0.0
 Identities = 329/405 (81%), Positives = 364/405 (89%)
 Frame = -2

Query: 1273 SLGLATRPDFPILHQEINGSKLVYLDNAATSQKPTAVLNALQNYYEAYNSNVHRGVHFLS 1094
            SLG   R DF ILHQE+NGSKLVYLD+AATSQKP AVL+ALQNYYE YNSNVHRG+H+LS
Sbjct: 52   SLGHRVRKDFRILHQEVNGSKLVYLDSAATSQKPAAVLDALQNYYEFYNSNVHRGIHYLS 111

Query: 1093 AKATDAYELARKKVATFINAPDSRDIVFTRNATEAINLVAYSWGLLNLKPDDEVVLTIAE 914
            AKATD +ELARKKVA FINA DSR+IVFTRNATEAINLVAYSWGL NLKP DEV+LT+AE
Sbjct: 112  AKATDEFELARKKVARFINASDSREIVFTRNATEAINLVAYSWGLSNLKPGDEVILTVAE 171

Query: 913  HHSAVVPWQLVAQKTGAVLKFVSLTDDEVPDLVELRGLLSVKTKLVVLHHVSNVLASVLP 734
            HHS +VPWQ+V+QKTGAVLKFV+L +DEVPD+ +LR L+S KTKLV +HHVSNVLAS LP
Sbjct: 172  HHSCIVPWQIVSQKTGAVLKFVTLNEDEVPDINKLRELISPKTKLVAVHHVSNVLASSLP 231

Query: 733  IEEIVLWAHDVGAKVLVDACQSVPHMVVDVKSLNADFLVASSHKMCGPTGIGFLYGRSEL 554
            IEEIV+WAHDVGAKVLVDACQSVPHMVVDV+ LNADFLVASSHKMCGPTGIGFLYG+S+L
Sbjct: 232  IEEIVVWAHDVGAKVLVDACQSVPHMVVDVQKLNADFLVASSHKMCGPTGIGFLYGKSDL 291

Query: 553  LAAMPPFLGGGEMIADVFLDHSTYAEPPSRFEAGTPAIGEAVGFGAAIDYLSSIGMQKIH 374
            L +MPPFLGGGEMI+DVFLDHSTYAEPPSRFEAGTPAIGEA+  GAA+DYLS IGM KIH
Sbjct: 292  LHSMPPFLGGGEMISDVFLDHSTYAEPPSRFEAGTPAIGEAIALGAAVDYLSGIGMPKIH 351

Query: 373  DYEVELANYLYGSLKEVPNVRIYGPAPSKHVTRAALCCFNVEDIHPTDIATFLDQQHGVA 194
            +YEVE+  YLY  L  +P+VRIYGP PS+ V R ALC FNVE +HPTD+ATFLDQQHGVA
Sbjct: 352  EYEVEIGKYLYEKLSSLPDVRIYGPRPSESVHRGALCSFNVEGLHPTDLATFLDQQHGVA 411

Query: 193  IRSGHHCAQPLHRYLGINASARASLYFYNTKQDVDDFIDALKDTI 59
            IRSGHHCAQPLHRYLG+NASARASLYFYNTK DVD FI AL DT+
Sbjct: 412  IRSGHHCAQPLHRYLGVNASARASLYFYNTKDDVDAFIVALADTV 456