BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003228
         (1179 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265166.1| PREDICTED: 2-oxoisovalerate dehydrogenase su...   442   e-122
ref|XP_002873392.1| hypothetical protein ARALYDRAFT_350155 [Arab...   408   e-111
ref|XP_004163044.1| PREDICTED: 2-oxoisovalerate dehydrogenase su...   405   e-111
ref|XP_004148662.1| PREDICTED: 2-oxoisovalerate dehydrogenase su...   405   e-111
ref|NP_568209.1| 2-oxoisovalerate dehydrogenase E1 component, al...   403   e-110

>ref|XP_002265166.1| PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha,
            mitochondrial [Vitis vinifera]
            gi|296088533|emb|CBI37524.3| unnamed protein product
            [Vitis vinifera]
          Length = 481

 Score =  442 bits (1138), Expect = e-122
 Identities = 219/331 (66%), Positives = 262/331 (79%), Gaps = 5/331 (1%)
 Frame = +2

Query: 200  MAIQLLKSKNVVSHLRSKLGFLAITNQPTNYNSIFTHKIHTKSTRILQNLKNPHEFSCKS 379
            MA+ L KSK ++  L+SK+  + +  + + ++  F H   +    +    +NP      +
Sbjct: 1    MALPLGKSKRILQCLKSKICVVGL-GKNSFWSGWFHHGCPSSMAPLAPTCRNPDAVLVNA 59

Query: 380  PTLLASHRWESTMAEKHLNFTEHA-----MDYPGGKVKYTSDLSFISGSTEERIHCYRVL 544
             T  A+ R+EST AEKH++    A     +D+PGGKV +TS++ FI  S  ER HCYRVL
Sbjct: 60   ATQFATRRFESTKAEKHVDSLCDAEGNQLLDFPGGKVAFTSEMRFIPESPMERAHCYRVL 119

Query: 545  DDSGQIIRDSNYIEISKDVAVKMYTDMVTLQVMDTVFYEAQRQGRISFYVTSIGEEAINI 724
            DD+GQ    SN++++SK+VAVKMY  M+TLQVMDT+FYEAQRQGRISFYVTSIGEEAIN+
Sbjct: 120  DDNGQP-NMSNFVQVSKEVAVKMYATMLTLQVMDTIFYEAQRQGRISFYVTSIGEEAINV 178

Query: 725  GSAAALQFQDLVFPQYREPGVLLWRGFTIQEFANQCFGNKADYGKGKQMPIHYGSNKHNY 904
             SAAAL   D++FPQYREPGVLLWRGFT+QEFANQCFGNKADYGKG+QMPIHYGSNKHNY
Sbjct: 179  ASAAALSIDDVIFPQYREPGVLLWRGFTLQEFANQCFGNKADYGKGRQMPIHYGSNKHNY 238

Query: 905  VTVSSTVGTQIPQAVGAAYSLKMDRKDACTITYFGDGGSSTGDFHAGLNFAAVMEAPVIF 1084
            VTVSSTV TQIPQAVGAAYSLKMD KDACT+TYFGDGGSSTGDFHA LNFAAVMEAPVIF
Sbjct: 239  VTVSSTVATQIPQAVGAAYSLKMDGKDACTVTYFGDGGSSTGDFHAALNFAAVMEAPVIF 298

Query: 1085 FCRNNGWAISTPVADQFRSDGIAIRGQAYGI 1177
             CRNNGWAISTPV+DQFRSDG+ +RG+ YG+
Sbjct: 299  ICRNNGWAISTPVSDQFRSDGVVVRGRGYGV 329


>ref|XP_002873392.1| hypothetical protein ARALYDRAFT_350155 [Arabidopsis lyrata subsp.
            lyrata] gi|297319229|gb|EFH49651.1| hypothetical protein
            ARALYDRAFT_350155 [Arabidopsis lyrata subsp. lyrata]
          Length = 472

 Score =  408 bits (1048), Expect = e-111
 Identities = 209/330 (63%), Positives = 253/330 (76%), Gaps = 17/330 (5%)
 Frame = +2

Query: 239  HLRS----KLGFLAITNQPTNYNSIFTHKIHTKSTRILQNLKNPHEFSCKSPTLLASH-- 400
            HLRS    K  FL I  Q   + S    + H  +  I QNL  PH+     P L  S   
Sbjct: 4    HLRSSPSLKSTFLNILRQNLGFGS----RSHV-TRHIRQNL--PHD-----PPLRGSQNP 51

Query: 401  --RWESTMAEKHL--NFTEH-------AMDYPGGKVKYTSDLSFISGSTEERIHCYRVLD 547
              R+ +TMAE     +F +H        MD+PGGKV +T ++ FIS S  ER+ CYRVLD
Sbjct: 52   IRRFCNTMAEPETLSSFGQHEDARNHQVMDFPGGKVAFTPEIRFISESDSERVPCYRVLD 111

Query: 548  DSGQIIRDSNYIEISKDVAVKMYTDMVTLQVMDTVFYEAQRQGRISFYVTSIGEEAINIG 727
            D+GQ+I +S ++++S++VAVK+Y+DMVTLQ+MD +FYEAQRQGR+SFY T+IGEEAINI 
Sbjct: 112  DNGQLITNSQFVQVSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIA 171

Query: 728  SAAALQFQDLVFPQYREPGVLLWRGFTIQEFANQCFGNKADYGKGKQMPIHYGSNKHNYV 907
            SAAAL  QD++FPQYREPGVLLWRGFT+QEFANQCFGNK+DYGKG+QMP+HYGSNK NY 
Sbjct: 172  SAAALTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYF 231

Query: 908  TVSSTVGTQIPQAVGAAYSLKMDRKDACTITYFGDGGSSTGDFHAGLNFAAVMEAPVIFF 1087
            TVS+T+ TQ+P AVGAAYSLKMDRKDAC +TYFGDGG+S GDFHA LNFAAVMEAPVIF 
Sbjct: 232  TVSATIATQLPNAVGAAYSLKMDRKDACAVTYFGDGGTSEGDFHAALNFAAVMEAPVIFI 291

Query: 1088 CRNNGWAISTPVADQFRSDGIAIRGQAYGI 1177
            CRNNGWAISTP +DQFRSDG+ ++G+AYGI
Sbjct: 292  CRNNGWAISTPTSDQFRSDGVVVKGRAYGI 321


>ref|XP_004163044.1| PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha,
            mitochondrial-like [Cucumis sativus]
          Length = 481

 Score =  405 bits (1042), Expect = e-111
 Identities = 201/338 (59%), Positives = 250/338 (73%), Gaps = 12/338 (3%)
 Frame = +2

Query: 200  MAIQLLKSKN-VVSHLRSKLGFLAITNQPTNYNSIFTH------KIHTKSTRILQNLKNP 358
            MA Q+ K+   ++ H R+ +GF     +   +NS  TH        H   TRIL      
Sbjct: 1    MAFQVRKATTKIIPHFRNGMGFFLAPLEMGFFNSYSTHLRNTSFSAHKIGTRILG----- 55

Query: 359  HEFSCKSPTLLASHRWESTMAEKHLNFT-----EHAMDYPGGKVKYTSDLSFISGSTEER 523
               S   PT L S R+ES    K LN       +  +++PGGKVK+TS LSFI    EER
Sbjct: 56   ---SENMPTHLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEER 112

Query: 524  IHCYRVLDDSGQIIRDSNYIEISKDVAVKMYTDMVTLQVMDTVFYEAQRQGRISFYVTSI 703
            I C+RVLDD+G+    +N+ ++SK+VA+K+Y DMVTLQ MDT+FYEAQRQGRISFYVTS+
Sbjct: 113  IPCFRVLDDNGRPCMYNNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSL 172

Query: 704  GEEAINIGSAAALQFQDLVFPQYREPGVLLWRGFTIQEFANQCFGNKADYGKGKQMPIHY 883
            GEEAIN+ SAAAL   DL+ PQYRE GVL+WRGFT++EFA+QCFGNK DY KG+QMP+HY
Sbjct: 173  GEEAINVASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHY 232

Query: 884  GSNKHNYVTVSSTVGTQIPQAVGAAYSLKMDRKDACTITYFGDGGSSTGDFHAGLNFAAV 1063
            GS +HNY TV+ST+ +QIP AVGAAYS+KMD+KDAC +TYFGDGG+S GDFHA LNFAAV
Sbjct: 233  GSKRHNYFTVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAV 292

Query: 1064 MEAPVIFFCRNNGWAISTPVADQFRSDGIAIRGQAYGI 1177
            +EAPVIF CRNNGWAISTP++DQFRSDG+ ++GQAYG+
Sbjct: 293  LEAPVIFICRNNGWAISTPISDQFRSDGVVVKGQAYGV 330


>ref|XP_004148662.1| PREDICTED: 2-oxoisovalerate dehydrogenase subunit alpha,
            mitochondrial-like [Cucumis sativus]
          Length = 481

 Score =  405 bits (1042), Expect = e-111
 Identities = 202/338 (59%), Positives = 250/338 (73%), Gaps = 12/338 (3%)
 Frame = +2

Query: 200  MAIQLLKSKN-VVSHLRSKLGFLAITNQPTNYNSIFTH------KIHTKSTRILQNLKNP 358
            MA Q+ K+   ++ H R+ +GF     +   +NS  TH        H   TRIL      
Sbjct: 1    MAFQVRKATTKIIPHFRNGMGFFLGPLEMGFFNSYSTHLRNTSFSAHKIGTRILG----- 55

Query: 359  HEFSCKSPTLLASHRWESTMAEKHLNFT-----EHAMDYPGGKVKYTSDLSFISGSTEER 523
               S   PT L S R+ES    K LN       +  +++PGGKVK+TS LSFI    EER
Sbjct: 56   ---SENMPTHLFSRRFESIKVGKQLNCVNDDRDDQVLEFPGGKVKFTSQLSFIPDREEER 112

Query: 524  IHCYRVLDDSGQIIRDSNYIEISKDVAVKMYTDMVTLQVMDTVFYEAQRQGRISFYVTSI 703
            I C+RVLDD+G+    SN+ ++SK+VA+K+Y DMVTLQ MDT+FYEAQRQGRISFYVTS+
Sbjct: 113  IPCFRVLDDNGRPCMYSNFTQVSKEVAMKIYHDMVTLQTMDTIFYEAQRQGRISFYVTSL 172

Query: 704  GEEAINIGSAAALQFQDLVFPQYREPGVLLWRGFTIQEFANQCFGNKADYGKGKQMPIHY 883
            GEEAIN+ SAAAL   DL+ PQYRE GVL+WRGFT++EFA+QCFGNK DY KG+QMP+HY
Sbjct: 173  GEEAINVASAAALSLDDLIVPQYREAGVLIWRGFTLKEFASQCFGNKYDYCKGRQMPVHY 232

Query: 884  GSNKHNYVTVSSTVGTQIPQAVGAAYSLKMDRKDACTITYFGDGGSSTGDFHAGLNFAAV 1063
            GS +HNY TV+ST+ +QIP AVGAAYS+KMD+KDAC +TYFGDGG+S GDFHA LNFAAV
Sbjct: 233  GSKRHNYFTVASTIASQIPHAVGAAYSMKMDKKDACAVTYFGDGGTSEGDFHAALNFAAV 292

Query: 1064 MEAPVIFFCRNNGWAISTPVADQFRSDGIAIRGQAYGI 1177
            +EAPVIF CRNNGWAISTP++DQFRSDG+ ++GQAYG+
Sbjct: 293  LEAPVIFICRNNGWAISTPISDQFRSDGVVVKGQAYGV 330


>ref|NP_568209.1| 2-oxoisovalerate dehydrogenase E1 component, alpha subunit
            [Arabidopsis thaliana] gi|28393751|gb|AAO42286.1|
            putative branched-chain alpha keto-acid dehydrogenase E1
            alpha subunit [Arabidopsis thaliana]
            gi|28973423|gb|AAO64036.1| putative branched-chain alpha
            keto-acid dehydrogenase E1 alpha subunit [Arabidopsis
            thaliana] gi|332003988|gb|AED91371.1| 2-oxoisovalerate
            dehydrogenase E1 component, alpha subunit [Arabidopsis
            thaliana]
          Length = 472

 Score =  403 bits (1035), Expect = e-110
 Identities = 196/330 (59%), Positives = 249/330 (75%), Gaps = 4/330 (1%)
 Frame = +2

Query: 200  MAIQLLKS----KNVVSHLRSKLGFLAITNQPTNYNSIFTHKIHTKSTRILQNLKNPHEF 367
            MA+ L  S      +++ LR  LGF + ++   +   I  H         L+  +NP   
Sbjct: 1    MALHLRSSFSSKSTLLNILRHNLGFGSRSHVTRHIRQILPHDPP------LRGSQNPISR 54

Query: 368  SCKSPTLLASHRWESTMAEKHLNFTEHAMDYPGGKVKYTSDLSFISGSTEERIHCYRVLD 547
             C +   +A     S+  +         MD+PGGKV +T ++ FIS S +ER+ CYRVLD
Sbjct: 55   LCNT---MAEPETLSSFVQHEYANNHQVMDFPGGKVAFTPEIQFISESDKERVPCYRVLD 111

Query: 548  DSGQIIRDSNYIEISKDVAVKMYTDMVTLQVMDTVFYEAQRQGRISFYVTSIGEEAINIG 727
            D+GQ+I +S ++++S++VAVK+Y+DMVTLQ+MD +FYEAQRQGR+SFY T+IGEEAINI 
Sbjct: 112  DNGQLITNSQFVQVSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRLSFYATAIGEEAINIA 171

Query: 728  SAAALQFQDLVFPQYREPGVLLWRGFTIQEFANQCFGNKADYGKGKQMPIHYGSNKHNYV 907
            SAAAL  QD++FPQYREPGVLLWRGFT+QEFANQCFGNK+DYGKG+QMP+HYGSNK NY 
Sbjct: 172  SAAALTPQDVIFPQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYF 231

Query: 908  TVSSTVGTQIPQAVGAAYSLKMDRKDACTITYFGDGGSSTGDFHAGLNFAAVMEAPVIFF 1087
            TVS+T+ TQ+P AVGAAYSLKMD+KDAC +TYFGDGG+S GDFHA LN AAVMEAPV+F 
Sbjct: 232  TVSATIATQLPNAVGAAYSLKMDKKDACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFI 291

Query: 1088 CRNNGWAISTPVADQFRSDGIAIRGQAYGI 1177
            CRNNGWAISTP +DQFRSDG+ ++G+AYGI
Sbjct: 292  CRNNGWAISTPTSDQFRSDGVVVKGRAYGI 321