BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003215
         (1400 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAN70903.1| hypothetical protein VITISV_028215 [Vitis vinifera]   544   e-152
ref|XP_002273303.1| PREDICTED: uncharacterized protein LOC100265...   540   e-151
ref|XP_002301401.1| predicted protein [Populus trichocarpa] gi|2...   525   e-147
ref|XP_004143876.1| PREDICTED: uncharacterized protein LOC101211...   522   e-145
ref|XP_002320186.1| predicted protein [Populus trichocarpa] gi|2...   520   e-145

>emb|CAN70903.1| hypothetical protein VITISV_028215 [Vitis vinifera]
          Length = 420

 Score =  544 bits (1401), Expect = e-152
 Identities = 256/427 (59%), Positives = 315/427 (73%), Gaps = 9/427 (2%)
 Frame = -3

Query: 1284 MKLNLKSWWYFVRKNNHWVVKLAVAFVIVGLASRLLSSRSTQFPSIGEEPSFLENSRAQS 1105
            M LN + W   + K+NH +VK+AV+ +++GLA RLL   ST    + E P F+E     +
Sbjct: 1    MGLNWRLW--SLHKHNHLLVKVAVSILLMGLAFRLLFVHST----VPETP-FVEKPVIPN 53

Query: 1104 P--------FRDQIHQKGECDLTVGNWIRNPEGPLYTNQSCQYIEDHQNCMKNGRPDTEY 949
            P         RD I +K +CDL  G+WI NP GP+YTN+SCQ IE HQNCMKNGRPD+ Y
Sbjct: 54   PPVSEDVPEIRDPIPEKEKCDLFTGDWIPNPSGPVYTNESCQLIETHQNCMKNGRPDSAY 113

Query: 948  LYWRWKPKDCELPQFDGKKFLEMMRNKAWALIGDSISRNHVQSLLCLLAKVEQPLHVYHD 769
            LYWRW P+DCELPQFD  +FLE+MRNKAWALIGDSI+RNHVQSLLC+L+KVEQP HVYHD
Sbjct: 114  LYWRWSPRDCELPQFDPYRFLELMRNKAWALIGDSITRNHVQSLLCILSKVEQPDHVYHD 173

Query: 768  ADYKNRRWVFSSYNFTISLVWSPFLAKANIFEDYNGVSSSEIELHMDVLDPIWTSMFHNL 589
              YK+RRWVF SYNFTIS++WSPFL +A IFED NGVS+SE+ELH+D LD  W   + NL
Sbjct: 174  KGYKSRRWVFPSYNFTISVLWSPFLVEAAIFEDMNGVSTSEVELHLDQLDKKWVDQYQNL 233

Query: 588  DYIIFSSGKWFVKTAIYYENNTIQGCHYC-QRNLTELGIQHAYRKVISNVFNLVIKSNHK 412
            +Y+I S+GKWF+K  IYYEN T+ GCH C  +NLT LG   AY+K +S VFN ++ S HK
Sbjct: 234  NYMIISTGKWFLKNTIYYENGTVVGCHSCSNKNLTXLGFDFAYQKALSFVFNFIVTSKHK 293

Query: 411  GMIFYRNPTPDHFENGEWFSGGTCQRKKPAEEGDFQYXXXXXXXXXXXXXEFAKAEVRAA 232
            GMI +R  TPDHFE+GEWFSGG C + +P +EG+ ++             EFAKAE +A 
Sbjct: 294  GMILFRTATPDHFESGEWFSGGVCPKAEPVKEGEIEFKGLHSILRDIELEEFAKAEKKAQ 353

Query: 231  EAGVKLKLLDVTPLSLLRPDGHPGPYRHFHPFAKDNTEKIINDCLHWCLPGPIDSWNDLL 52
            E GV LKLLD++ +SLLRPDGHPGPYR F+PFA D    + NDCLHWCLPGPIDSWND++
Sbjct: 354  ENGVNLKLLDLSKISLLRPDGHPGPYRFFYPFASDKNATVQNDCLHWCLPGPIDSWNDVI 413

Query: 51   MEMIVKG 31
            MEM+V G
Sbjct: 414  MEMVVNG 420


>ref|XP_002273303.1| PREDICTED: uncharacterized protein LOC100265666 [Vitis vinifera]
            gi|297742310|emb|CBI34459.3| unnamed protein product
            [Vitis vinifera]
          Length = 427

 Score =  540 bits (1391), Expect = e-151
 Identities = 257/432 (59%), Positives = 316/432 (73%), Gaps = 12/432 (2%)
 Frame = -3

Query: 1290 KEMKLNLKSWWYFVRKNNHWVVKLAVAFVIVGLASRLLSSRSTQFPSIGEEPSFLENSRA 1111
            K M LN + W   + K+NH +VK+AV+ +++GLA RLL   ST    + E P F+E    
Sbjct: 3    KNMGLNWRLW--SLHKHNHLLVKVAVSILLMGLAFRLLFVHST----VPETP-FVEKPVI 55

Query: 1110 QSP--------FRDQIHQK---GECDLTVGNWIRNPEGPLYTNQSCQYIEDHQNCMKNGR 964
             +P         RD I +K    +CDL  G+WI NP GP+YTN+SCQ IE HQNCMKNGR
Sbjct: 56   PNPPVSEDVPEIRDPIPEKVVAEKCDLFTGDWIPNPSGPVYTNESCQLIETHQNCMKNGR 115

Query: 963  PDTEYLYWRWKPKDCELPQFDGKKFLEMMRNKAWALIGDSISRNHVQSLLCLLAKVEQPL 784
            PD+ YLYWRW P+DCELPQFD  +FLE+MRNKAWALIGDSI+RNHVQSLLC+L+KVEQP 
Sbjct: 116  PDSAYLYWRWSPRDCELPQFDPYRFLELMRNKAWALIGDSITRNHVQSLLCILSKVEQPD 175

Query: 783  HVYHDADYKNRRWVFSSYNFTISLVWSPFLAKANIFEDYNGVSSSEIELHMDVLDPIWTS 604
            HVYHD  YK+RRWVF SYNFTIS++WSPFL +A IFED NGVS+SE+ELH+D LD  W  
Sbjct: 176  HVYHDKGYKSRRWVFPSYNFTISVLWSPFLVEAAIFEDMNGVSTSEVELHLDQLDKKWVD 235

Query: 603  MFHNLDYIIFSSGKWFVKTAIYYENNTIQGCHYC-QRNLTELGIQHAYRKVISNVFNLVI 427
             + NL+Y+I S+GKWF+K  IYYEN T+ GCH C  +NLT LG   AY+K +S VFN ++
Sbjct: 236  QYQNLNYMIISTGKWFLKNTIYYENGTVVGCHSCSNKNLTVLGFDFAYQKALSFVFNFIV 295

Query: 426  KSNHKGMIFYRNPTPDHFENGEWFSGGTCQRKKPAEEGDFQYXXXXXXXXXXXXXEFAKA 247
             S HKGMI +R  TPDHFE+GEWFSGG C + +P +EG+ ++             EFAKA
Sbjct: 296  TSKHKGMILFRTATPDHFESGEWFSGGVCPKAEPVKEGEIEFKGLHSILRDIELEEFAKA 355

Query: 246  EVRAAEAGVKLKLLDVTPLSLLRPDGHPGPYRHFHPFAKDNTEKIINDCLHWCLPGPIDS 67
            E +A E GV LKLLD++ +SLLRPDGHPGPYR F+PFA D    + NDCLHWCLPGPIDS
Sbjct: 356  EKKAQENGVNLKLLDLSKISLLRPDGHPGPYRFFYPFASDKNATVQNDCLHWCLPGPIDS 415

Query: 66   WNDLLMEMIVKG 31
            WND++MEM+V G
Sbjct: 416  WNDVIMEMVVNG 427


>ref|XP_002301401.1| predicted protein [Populus trichocarpa] gi|222843127|gb|EEE80674.1|
            predicted protein [Populus trichocarpa]
          Length = 441

 Score =  525 bits (1353), Expect = e-147
 Identities = 251/441 (56%), Positives = 308/441 (69%), Gaps = 17/441 (3%)
 Frame = -3

Query: 1290 KEMKLNLKSWWYFVRKNNHWVVKLAVAFVIVGLASRLLSSRSTQFPSIGE---------E 1138
            KEM+ +L    + + K+N  ++K AV+F++VGLA RLL S S +F S+ E         +
Sbjct: 3    KEMRSDLNP--FSLSKHNSVIIKFAVSFLLVGLAFRLLISGSFRFSSVVETSVPANEETK 60

Query: 1137 PSFLENSRAQSPF-------RDQIHQKGECDLTVGNWIRNPEGPLYTNQSCQYIEDHQNC 979
            P  L  S  + P        + Q  Q G+CDL  G WI +P GP YTNQSC  IE HQNC
Sbjct: 61   PESLMASLPEEPASNDFEANKSQNSQSGKCDLFTGEWIPDPSGPFYTNQSCLQIEGHQNC 120

Query: 978  MKNGRPDTEYLYWRWKPKDCELPQFDGKKFLEMMRNKAWALIGDSISRNHVQSLLCLLAK 799
            MKNGRPD+ YLYWRW P+ C LP+F+ KKFL +MRNK+WA IGDSISRNHVQSLLC+L++
Sbjct: 121  MKNGRPDSGYLYWRWSPRGCSLPKFNPKKFLHLMRNKSWAFIGDSISRNHVQSLLCILSQ 180

Query: 798  VEQPLHVYHDADYKNRRWVFSSYNFTISLVWSPFLAKANIFEDYNGVSSSEIELHMDVLD 619
            VEQ +  YHD +Y+++ W F ++NFT+S++W+PFL KA+IFED NGVSSSEI+LH+D LD
Sbjct: 181  VEQAVETYHDEEYRSKIWHFRTHNFTLSVIWTPFLIKADIFEDMNGVSSSEIQLHLDELD 240

Query: 618  PIWTSMFHNLDYIIFSSGKWFVKTAIYYENNTIQGCHYCQ-RNLTELGIQHAYRKVISNV 442
              WT  + N DY I + GKWF+KTAIY+ENN + GCHYC  +NLTELG  +AYRK I  +
Sbjct: 241  KKWTDQYRNFDYAIVAGGKWFLKTAIYHENNVVTGCHYCPGKNLTELGFDYAYRKAIQLI 300

Query: 441  FNLVIKSNHKGMIFYRNPTPDHFENGEWFSGGTCQRKKPAEEGDFQYXXXXXXXXXXXXX 262
            FN +  S HK + F R  TPDHFENGEWFSGGTC RK P +EG+                
Sbjct: 301  FNFITNSGHKTLAFLRTTTPDHFENGEWFSGGTCDRKVPFKEGEVNMTDVDTIMRNIELE 360

Query: 261  EFAKAEVRAAEAGVKLKLLDVTPLSLLRPDGHPGPYRHFHPFAKDNTEKIINDCLHWCLP 82
            EFAKA     + GV LKLLD T LSLLRPDGHPGPYR F PFA+D   K+ NDCLHWCLP
Sbjct: 361  EFAKAAALGPDKGVVLKLLDTTRLSLLRPDGHPGPYRQFQPFAEDKNAKVQNDCLHWCLP 420

Query: 81   GPIDSWNDLLMEMIVKG*VYQ 19
            GPIDSWNDL+MEMIV G +YQ
Sbjct: 421  GPIDSWNDLVMEMIVNGGIYQ 441


>ref|XP_004143876.1| PREDICTED: uncharacterized protein LOC101211100 [Cucumis sativus]
            gi|449501804|ref|XP_004161463.1| PREDICTED:
            uncharacterized LOC101211100 [Cucumis sativus]
          Length = 450

 Score =  522 bits (1344), Expect = e-145
 Identities = 252/451 (55%), Positives = 314/451 (69%), Gaps = 33/451 (7%)
 Frame = -3

Query: 1284 MKLNLKSWWYFVRKNNHWVVKLAVAFVIVGLASRLLSSRSTQFPSIGEEP--------SF 1129
            MK N K    F ++N+  ++KL +  +++G+A R L + S   P+I E P        + 
Sbjct: 1    MKFNWKPPVPF-KRNHVLILKLVLPILLLGIAFRFLFAGSGDVPAILETPLTERLAAPTI 59

Query: 1128 LEN---SRAQSPF---------------------RDQIHQKGECDLTVGNWIRNPEGPLY 1021
            LEN    + Q P                       +Q   +G+C+L VG WI NP GP+Y
Sbjct: 60   LENPLIDKKQVPAILETPLSKKSEDLEEPETPEDENQKPLEGKCNLFVGEWIPNPSGPVY 119

Query: 1020 TNQSCQYIEDHQNCMKNGRPDTEYLYWRWKPKDCELPQFDGKKFLEMMRNKAWALIGDSI 841
             N+SC +IE HQNCMKNGRPDTEYLYWRWKP+DCEL +FD KKFLE MR+K WALIGDSI
Sbjct: 120  NNESCPHIEAHQNCMKNGRPDTEYLYWRWKPRDCELSKFDPKKFLERMRDKTWALIGDSI 179

Query: 840  SRNHVQSLLCLLAKVEQPLHVYHDADYKNRRWVFSSYNFTISLVWSPFLAKANIFEDYNG 661
            SRNHVQSLLC+L+ VE  +H+YHD  YKNR+W F SYNFTIS+VWSPFLA+A IFEDYNG
Sbjct: 180  SRNHVQSLLCMLSTVELAVHIYHDEGYKNRKWRFPSYNFTISVVWSPFLAEAAIFEDYNG 239

Query: 660  VSSSEIELHMDVLDPIWTSMFHNLDYIIFSSGKWFVKTAIYYENNTIQGCHYC-QRNLTE 484
            VS+SE+ELH+D LD  WTS FH +DY+I SSGKWFVK+AIYYE+N + GCH C ++N TE
Sbjct: 240  VSTSEVELHLDKLDKKWTSEFHQMDYVIISSGKWFVKSAIYYEHNKVLGCHTCPKKNFTE 299

Query: 483  LGIQHAYRKVISNVFNLVIKSNHKGMIFYRNPTPDHFENGEWFSGGTCQRKKPAEEGDFQ 304
            LGI+ AYR+ + +  N ++ S HKGMIF R  TPDHFENG W  GGTC R+ P +EG+ +
Sbjct: 300  LGIEFAYRRSLRSALNFILASKHKGMIFMRTSTPDHFENGTWSDGGTCDRRAPIKEGEME 359

Query: 303  YXXXXXXXXXXXXXEFAKAEVRAAEAGVKLKLLDVTPLSLLRPDGHPGPYRHFHPFAKDN 124
                          EF KAEV+A++ G+ LKLLD+T LSLLRPDGHPGPYR F PFA D 
Sbjct: 360  LKELTKILRIVELEEFKKAEVKASQNGLNLKLLDLTQLSLLRPDGHPGPYREFQPFANDK 419

Query: 123  TEKIINDCLHWCLPGPIDSWNDLLMEMIVKG 31
              K+  DCLHWCLPGPID+WND++MEM++ G
Sbjct: 420  NAKVQKDCLHWCLPGPIDAWNDVIMEMMLNG 450


>ref|XP_002320186.1| predicted protein [Populus trichocarpa] gi|222860959|gb|EEE98501.1|
            predicted protein [Populus trichocarpa]
          Length = 442

 Score =  520 bits (1338), Expect = e-145
 Identities = 248/427 (58%), Positives = 303/427 (70%), Gaps = 18/427 (4%)
 Frame = -3

Query: 1245 KNNHWVVKLAVAFVIVGLASRLLSSRSTQFPSIGE---------EP-SFLENSRAQSPFR 1096
            K+N+ ++K AV+F++VGLA  LL S S +F S+ E         EP S + +  A+ P  
Sbjct: 16   KHNNVIIKFAVSFLLVGLAFHLLISDSFRFSSVVETLVPANEETEPESLVASLPAEEPVS 75

Query: 1095 D-------QIHQKGECDLTVGNWIRNPEGPLYTNQSCQYIEDHQNCMKNGRPDTEYLYWR 937
            +       Q  Q G+CDL VG+WI +P GP YTNQSC  IE HQNCMKNGRPD+ YL+WR
Sbjct: 76   NDFAANESQTSQPGKCDLFVGDWIPDPSGPFYTNQSCLQIEGHQNCMKNGRPDSGYLHWR 135

Query: 936  WKPKDCELPQFDGKKFLEMMRNKAWALIGDSISRNHVQSLLCLLAKVEQPLHVYHDADYK 757
            W P+ C LP+F+ KKFL +MRNK+WA IGDSISRNHVQSLLC L++VEQ + VYHD +Y+
Sbjct: 136  WNPRGCSLPRFNPKKFLRLMRNKSWAFIGDSISRNHVQSLLCTLSQVEQAVEVYHDEEYR 195

Query: 756  NRRWVFSSYNFTISLVWSPFLAKANIFEDYNGVSSSEIELHMDVLDPIWTSMFHNLDYII 577
            +R W F S+NFT+S++W+PFL KA+IFED NGVSSSEI+LH+D LD  WT  + NLDY I
Sbjct: 196  SRIWRFPSHNFTLSVIWTPFLIKADIFEDMNGVSSSEIQLHLDKLDKKWTDQYRNLDYAI 255

Query: 576  FSSGKWFVKTAIYYENNTIQGCHYCQ-RNLTELGIQHAYRKVISNVFNLVIKSNHKGMIF 400
             + GKWF+KTAIY+ENN + GCHYC  +NLTELG  +AYRK +  +FN +  S HK + F
Sbjct: 256  IAGGKWFLKTAIYHENNVVTGCHYCPGKNLTELGFDYAYRKAMQLIFNFITNSGHKALAF 315

Query: 399  YRNPTPDHFENGEWFSGGTCQRKKPAEEGDFQYXXXXXXXXXXXXXEFAKAEVRAAEAGV 220
            +R  TPDHFENGEWFSGGTC RK P +E +                EF KA     E GV
Sbjct: 316  FRTTTPDHFENGEWFSGGTCNRKVPFKEDEVNMTDVDTIMHNIELEEFEKATALGPEKGV 375

Query: 219  KLKLLDVTPLSLLRPDGHPGPYRHFHPFAKDNTEKIINDCLHWCLPGPIDSWNDLLMEMI 40
             LKLLD T LSLLRPDGHPGPYR F PFA+D   K+ NDCLHWCLPGPIDSWNDL+MEMI
Sbjct: 376  ALKLLDTTRLSLLRPDGHPGPYRQFQPFAEDKNAKVQNDCLHWCLPGPIDSWNDLVMEMI 435

Query: 39   VKG*VYQ 19
            V G + Q
Sbjct: 436  VNGGIDQ 442