BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003155
         (1211 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAN82127.1| hypothetical protein VITISV_044128 [Vitis vinifera]   556   e-156
ref|XP_002275678.1| PREDICTED: L-ascorbate oxidase-like [Vitis v...   556   e-156
sp|Q40588.1|ASO_TOBAC RecName: Full=L-ascorbate oxidase; Short=A...   551   e-154
ref|XP_002312838.1| l-ascorbate oxidase precursor [Populus trich...   531   e-148
gb|ABK96191.1| unknown [Populus trichocarpa]                          524   e-146

>emb|CAN82127.1| hypothetical protein VITISV_044128 [Vitis vinifera]
          Length = 558

 Score =  556 bits (1434), Expect = e-156
 Identities = 255/353 (72%), Positives = 292/353 (82%)
 Frame = -2

Query: 1201 ECKFSGTEQCAPQYMTVEPNKIYRLRIXXXXXXXXXXXAIGKHKMVVVEADGNYVQPFSV 1022
            +C+F G EQCAPQ + V+P+K YRLR+            IG HKMV+VEADGNY+QP +V
Sbjct: 198  QCQFRGNEQCAPQILHVQPHKTYRLRVSSTTALASLNLQIGNHKMVMVEADGNYIQPVAV 257

Query: 1021 YDMDIYSGESYSVLFKADQSPLQNYWISVGVRGRKPNTPQALTILNYAPTSHSNLPTSRP 842
             D+DIYSGESYSVL   DQ P  NYWISV VRGR+P TPQ LTILNY  TS S LPTS P
Sbjct: 258  DDLDIYSGESYSVLIHTDQDPSXNYWISVSVRGREPKTPQGLTILNYQTTSASKLPTSTP 317

Query: 841  PVTPLWNDYDHSKSFTNRIKALRGSPKPPSTYNRRIMLLNTQNKINGFTKWAINNVSLSL 662
            PV+PLWNDY+HSKSF+N+I AL GSPKPP+TYNRRI+LLNTQN INGFTKWAINN+SL+L
Sbjct: 318  PVSPLWNDYNHSKSFSNKILALMGSPKPPTTYNRRIILLNTQNTINGFTKWAINNISLTL 377

Query: 661  PVTPYLGAIKYNINNAFANSPPPESYSPAYDVMRPPVNPNSTYGDGVYMLKFNTTIDVIL 482
            P TPYLGAIK+ ++NAF    PPE++   YDVM+PP N NSTYG+GVYML+F TT+DVIL
Sbjct: 378  PPTPYLGAIKHRLSNAFDQKSPPENFPNDYDVMKPPTNTNSTYGNGVYMLEFRTTVDVIL 437

Query: 481  QNANALSDNVSEIHPWHLHGHDFWVLGYGEQKFTDKDVTKFNLNNPPLRNSAVIFPYGWT 302
            QNANAL+  VSEIHPWHLHGHDFWVLGYGE KF +KD  +FNL NPPLRN+AVIFP+GWT
Sbjct: 438  QNANALATGVSEIHPWHLHGHDFWVLGYGEGKFREKDAKRFNLKNPPLRNTAVIFPFGWT 497

Query: 301  ALRFVADNPGAWAFHCHIEPHLHMGMGVVFAEGIYKVKNIPTEALTCGLTGKM 143
            ALRFVADNPG WAFHCHIEPHLHMGMGVVFAEG++ VK++P  AL CGLTGKM
Sbjct: 498  ALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHLVKDVPNHALACGLTGKM 550


>ref|XP_002275678.1| PREDICTED: L-ascorbate oxidase-like [Vitis vinifera]
          Length = 581

 Score =  556 bits (1433), Expect = e-156
 Identities = 254/353 (71%), Positives = 293/353 (83%)
 Frame = -2

Query: 1201 ECKFSGTEQCAPQYMTVEPNKIYRLRIXXXXXXXXXXXAIGKHKMVVVEADGNYVQPFSV 1022
            +C+F G EQC+PQ + V+P+K YRLR+            IG HKMV+VEADGNY+QP +V
Sbjct: 221  QCQFRGNEQCSPQILHVQPHKTYRLRVSSTTALASLNLQIGNHKMVMVEADGNYIQPVAV 280

Query: 1021 YDMDIYSGESYSVLFKADQSPLQNYWISVGVRGRKPNTPQALTILNYAPTSHSNLPTSRP 842
             D+DIYSGESYSVL   DQ P +NYWISV VRGR+P TPQ LTILNY  TS S LPTS P
Sbjct: 281  DDLDIYSGESYSVLIHTDQDPSKNYWISVSVRGREPKTPQGLTILNYQTTSASKLPTSTP 340

Query: 841  PVTPLWNDYDHSKSFTNRIKALRGSPKPPSTYNRRIMLLNTQNKINGFTKWAINNVSLSL 662
            PV+PLWNDY+HSKSF+N+I AL GSPKPP+TYNRRI+LLNTQN INGFTKWAINN+SL+L
Sbjct: 341  PVSPLWNDYNHSKSFSNKILALMGSPKPPTTYNRRIILLNTQNTINGFTKWAINNISLTL 400

Query: 661  PVTPYLGAIKYNINNAFANSPPPESYSPAYDVMRPPVNPNSTYGDGVYMLKFNTTIDVIL 482
            P TPYLGAIK+ ++NAF    PPE++   YDVM+PP N NSTYG+GVYML+F TT+DVIL
Sbjct: 401  PPTPYLGAIKHRLSNAFDQKSPPENFPNDYDVMKPPTNTNSTYGNGVYMLEFRTTVDVIL 460

Query: 481  QNANALSDNVSEIHPWHLHGHDFWVLGYGEQKFTDKDVTKFNLNNPPLRNSAVIFPYGWT 302
            QNANAL+  VSEIHPWHLHGHDFWVLGYGE KF +KD  +FNL NPPLRN+AVIFP+GWT
Sbjct: 461  QNANALATGVSEIHPWHLHGHDFWVLGYGEGKFREKDAKRFNLKNPPLRNTAVIFPFGWT 520

Query: 301  ALRFVADNPGAWAFHCHIEPHLHMGMGVVFAEGIYKVKNIPTEALTCGLTGKM 143
            ALRFVADNPG WAFHCHIEPHLHMGMGVVFAEG++ VK++P  AL CGLTGKM
Sbjct: 521  ALRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHLVKDVPNHALACGLTGKM 573


>sp|Q40588.1|ASO_TOBAC RecName: Full=L-ascorbate oxidase; Short=ASO; Short=Ascorbase; Flags:
            Precursor gi|599594|dbj|BAA07734.1| ascorbate oxidase
            precursor [Nicotiana tabacum]
          Length = 578

 Score =  551 bits (1420), Expect = e-154
 Identities = 259/362 (71%), Positives = 292/362 (80%)
 Frame = -2

Query: 1210 PLSECKFSGTEQCAPQYMTVEPNKIYRLRIXXXXXXXXXXXAIGKHKMVVVEADGNYVQP 1031
            PL +CK  G EQ APQ + V PNKIYRLR+           AIG HKMVVVEADGNYVQP
Sbjct: 217  PLPQCKLRGGEQYAPQILRVRPNKIYRLRVASTTALGSLSLAIGGHKMVVVEADGNYVQP 276

Query: 1030 FSVYDMDIYSGESYSVLFKADQSPLQNYWISVGVRGRKPNTPQALTILNYAPTSHSNLPT 851
            FSV DMDIYSGESYSVLFK DQ P +NYWIS+ VRGR+P TPQ LT+LNY P S S  PT
Sbjct: 277  FSVQDMDIYSGESYSVLFKTDQDPTKNYWISINVRGREPKTPQGLTLLNYLPNSASKFPT 336

Query: 850  SRPPVTPLWNDYDHSKSFTNRIKALRGSPKPPSTYNRRIMLLNTQNKINGFTKWAINNVS 671
              PP+ PLWNDY+HSKSF+N+I AL GSPKPP   +RRI+LLNTQNKI+G+TKWAINNVS
Sbjct: 337  LPPPIAPLWNDYNHSKSFSNKIFALMGSPKPPPQNHRRIILLNTQNKIDGYTKWAINNVS 396

Query: 670  LSLPVTPYLGAIKYNINNAFANSPPPESYSPAYDVMRPPVNPNSTYGDGVYMLKFNTTID 491
            L LP   YLG+I+Y IN AF   PPP+++   YDV++   N NSTYG+GVYMLKFNTTID
Sbjct: 397  LVLPTQLYLGSIRYGIN-AFDTKPPPDNFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTID 455

Query: 490  VILQNANALSDNVSEIHPWHLHGHDFWVLGYGEQKFTDKDVTKFNLNNPPLRNSAVIFPY 311
            +ILQNANAL+ +VSEIHPWHLHGHDFWVLGYGE KF++KDV KFNL NPPLRN+AVIFP+
Sbjct: 456  IILQNANALAKDVSEIHPWHLHGHDFWVLGYGEGKFSEKDVKKFNLKNPPLRNTAVIFPF 515

Query: 310  GWTALRFVADNPGAWAFHCHIEPHLHMGMGVVFAEGIYKVKNIPTEALTCGLTGKMFRGK 131
            GWTALRFV DNPG WAFHCHIEPHLHMGMGV+FAEG++ VK IP EAL CGLTGKM    
Sbjct: 516  GWTALRFVTDNPGVWAFHCHIEPHLHMGMGVIFAEGVHLVKKIPKEALACGLTGKMLMSN 575

Query: 130  KH 125
            KH
Sbjct: 576  KH 577


>ref|XP_002312838.1| l-ascorbate oxidase precursor [Populus trichocarpa]
            gi|222849246|gb|EEE86793.1| l-ascorbate oxidase precursor
            [Populus trichocarpa]
          Length = 597

 Score =  531 bits (1368), Expect = e-148
 Identities = 248/358 (69%), Positives = 286/358 (79%), Gaps = 2/358 (0%)
 Frame = -2

Query: 1207 LSECKFSGTEQCAPQYMTVEPNKIYRLRIXXXXXXXXXXXAIGKHKMVVVEADGNYVQPF 1028
            LS+C  +G EQ AP  + V+PNK YR+R+           AIG HKM+VVEADGNY+QPF
Sbjct: 233  LSQCNVTGHEQWAPYILHVDPNKTYRIRLSSTTALASLNLAIGNHKMLVVEADGNYLQPF 292

Query: 1027 SVYDMDIYSGESYSVLFKADQSPLQNYWISVGVRGRKPNTPQALTILNYAPTSHSNLPTS 848
               D+DIYSGESYSVL K  Q P QNYWIS GVRGRKP TPQALTILNY   S S  P S
Sbjct: 293  ETDDLDIYSGESYSVLLKTSQDPSQNYWISFGVRGRKPQTPQALTILNYKTNSASKFPLS 352

Query: 847  RPPVTPLWNDYDHSKSFTNRIKAL--RGSPKPPSTYNRRIMLLNTQNKINGFTKWAINNV 674
             PPVTP W+DY HSK+FTN++KAL  +  PKPPSTY+RRI+LLNTQNK+NG+TKW+INNV
Sbjct: 353  PPPVTPRWDDYAHSKAFTNKVKALDHKTIPKPPSTYHRRIILLNTQNKMNGYTKWSINNV 412

Query: 673  SLSLPVTPYLGAIKYNINNAFANSPPPESYSPAYDVMRPPVNPNSTYGDGVYMLKFNTTI 494
            SLSLP TPYLG+I++ + N F  + PPES+   YDVM+PP NPN+T G+GVYML + +T+
Sbjct: 413  SLSLPATPYLGSIRFGLQNGFDQTKPPESFPVQYDVMKPPGNPNTTTGNGVYMLSYYSTV 472

Query: 493  DVILQNANALSDNVSEIHPWHLHGHDFWVLGYGEQKFTDKDVTKFNLNNPPLRNSAVIFP 314
            DVILQNANAL++NVSEIHPWHLHGHDFWVLGYGE KFT  D  KFN+ NPP RNSAVIFP
Sbjct: 473  DVILQNANALAENVSEIHPWHLHGHDFWVLGYGEGKFTKDDEKKFNMKNPPYRNSAVIFP 532

Query: 313  YGWTALRFVADNPGAWAFHCHIEPHLHMGMGVVFAEGIYKVKNIPTEALTCGLTGKMF 140
            YGWTALRFVADNPG WAFHCHIEPHLHMGMGVV AEG+ ++  IP EAL+CGLTGK F
Sbjct: 533  YGWTALRFVADNPGVWAFHCHIEPHLHMGMGVVLAEGVQRLPKIPKEALSCGLTGKKF 590


>gb|ABK96191.1| unknown [Populus trichocarpa]
          Length = 594

 Score =  524 bits (1349), Expect = e-146
 Identities = 246/362 (67%), Positives = 285/362 (78%), Gaps = 2/362 (0%)
 Frame = -2

Query: 1204 SECKFSGTEQCAPQYMTVEPNKIYRLRIXXXXXXXXXXXAIGKHKMVVVEADGNYVQPFS 1025
            S+C  +G EQ AP  + V+PNK YR+RI           AIG HKM+VVEADGNY+QPF 
Sbjct: 231  SQCNITGHEQWAPYILHVDPNKTYRIRIASTTALASLNLAIGNHKMMVVEADGNYLQPFE 290

Query: 1024 VYDMDIYSGESYSVLFKADQSPLQNYWISVGVRGRKPNTPQALTILNYAPTSHSNLPTSR 845
              D+DIYSGESYSVL K  Q   QNYWIS GVRGR+P+TPQALTILNY   S S LP S 
Sbjct: 291  TNDLDIYSGESYSVLLKTSQDSSQNYWISFGVRGREPHTPQALTILNYKTNSASKLPLSP 350

Query: 844  PPVTPLWNDYDHSKSFTNRIKAL--RGSPKPPSTYNRRIMLLNTQNKINGFTKWAINNVS 671
            PPVTP W+DY HSK+F+N+IKAL  +  PKP  TY+RRI+LLNTQNK+NG+TKW+INNVS
Sbjct: 351  PPVTPRWDDYAHSKAFSNKIKALDHKSIPKPSPTYHRRIVLLNTQNKMNGYTKWSINNVS 410

Query: 670  LSLPVTPYLGAIKYNINNAFANSPPPESYSPAYDVMRPPVNPNSTYGDGVYMLKFNTTID 491
            LS+P TPYLG+I++ + +AF  + PPE +   YDVM+PPVNPN+T G GVYML + +T+D
Sbjct: 411  LSIPATPYLGSIRFGLRDAFDQTKPPEDFPGHYDVMKPPVNPNTTTGSGVYMLGYYSTVD 470

Query: 490  VILQNANALSDNVSEIHPWHLHGHDFWVLGYGEQKFTDKDVTKFNLNNPPLRNSAVIFPY 311
            VILQNANAL++NVSEIHPWHLHGHDFWVLGYGE KFT  D  KFNL NPP RN+AVIFPY
Sbjct: 471  VILQNANALTENVSEIHPWHLHGHDFWVLGYGEGKFTKSDEKKFNLKNPPYRNTAVIFPY 530

Query: 310  GWTALRFVADNPGAWAFHCHIEPHLHMGMGVVFAEGIYKVKNIPTEALTCGLTGKMFRGK 131
            GWTALRFVADNPG WAFHCHIEPHLH+GMGVVFAEG+  V  IP EAL+CGLTGK F   
Sbjct: 531  GWTALRFVADNPGVWAFHCHIEPHLHLGMGVVFAEGVQHVAKIPKEALSCGLTGKRFMTG 590

Query: 130  KH 125
             H
Sbjct: 591  SH 592