BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003138
         (1510 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265217.1| PREDICTED: pectinesterase 3 [Vitis vinifera]      471   e-149
ref|XP_002304256.1| predicted protein [Populus trichocarpa] gi|2...   465   e-149
ref|XP_002532945.1| Pectinesterase-3 precursor, putative [Ricinu...   457   e-145
gb|AFI23414.1| pectin methylesterase [Coffea arabica]                 434   e-137
ref|XP_004144445.1| PREDICTED: pectinesterase 3-like [Cucumis sa...   435   e-133

>ref|XP_002265217.1| PREDICTED: pectinesterase 3 [Vitis vinifera]
          Length = 578

 Score =  471 bits (1213), Expect(2) = e-149
 Identities = 234/327 (71%), Positives = 275/327 (84%), Gaps = 3/327 (0%)
 Frame = -2

Query: 972  KEAFKVCQIVLDDALERLNDTLSSMDVNESKGQKLLSVTKLDDLKTWLSATITDQETCLD 793
            K+A  VC+ V +DA++RLND++SSM+V E  G+KLLS +K+DD+KTWLSATITDQETCLD
Sbjct: 136  KKALGVCETVFEDAIDRLNDSISSMEVRE--GEKLLSASKIDDIKTWLSATITDQETCLD 193

Query: 792  ALDEFNATAL--VGDSLDKPTQYASNSLSIVAKILGVLARFNVPVHRRLLGIGGHG-FPE 622
            AL+E N+T L  V  ++   T +ASNSL+IVAK++G+L   ++ VHR+LL       FP+
Sbjct: 194  ALEELNSTLLNEVKTAMQNSTVFASNSLAIVAKLIGILHDLDIQVHRKLLSFSNSDQFPD 253

Query: 621  WVSSGDRRLLQEVRPKPDVTVAADGSGDVKTIREAAERIPKKSTKRFVIYVKAGEYLGNN 442
            WV +G+RRLLQE +P PDVTVA DG+GD  TI+EA   +PKKS KRFVIYVK G Y  N 
Sbjct: 254  WVGAGERRLLQETKPTPDVTVAKDGTGDYVTIKEAVAMVPKKSEKRFVIYVKEGNYSENI 313

Query: 441  ILDKSKWNVMMYGDGKGQTILSGSLNFIDGTPTFSTATFAAVGKGFIAKDMTFKNTAGAE 262
            ILDKSKWNVM+YGDGK ++I+SG+LNFIDGTPTF+TATFAAVGKGFIAK M F+NTAGA 
Sbjct: 314  ILDKSKWNVMIYGDGKDKSIVSGNLNFIDGTPTFATATFAAVGKGFIAKYMRFENTAGAA 373

Query: 261  KHQAVAFRSGSDFSVFYRCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNC 82
            KHQAVAFRSGSD SVFY+CSFDA+QDTLYAHSNRQFYR+CDITGTIDFIFGNAAVVFQ C
Sbjct: 374  KHQAVAFRSGSDMSVFYQCSFDAFQDTLYAHSNRQFYRECDITGTIDFIFGNAAVVFQAC 433

Query: 81   KIQPRQPMPNQFVTITAQGKKDPNQNT 1
            KIQPRQPM NQF TITAQGKKDPNQNT
Sbjct: 434  KIQPRQPMSNQFNTITAQGKKDPNQNT 460



 Score = 87.0 bits (214), Expect(2) = e-149
 Identities = 52/115 (45%), Positives = 64/115 (55%), Gaps = 2/115 (1%)
 Frame = -3

Query: 1406 MDSIQSFKGYGKVDPVEEAAFRKKSRKRXXXXXXXXXXXXXXXXXXXXXXXVHXXXXXXX 1227
            MDSI+SFKGYGKVD +EE AF++K+RKR                       +H       
Sbjct: 1    MDSIKSFKGYGKVDELEERAFKRKTRKRLIILIISSVVLVAVIIGAVAGTLIHKSKSESN 60

Query: 1226 XXXXSDPAQ--TSAAALNAVCKQTLYPDSCHSSISALNLKNTADPEELFRLSLRV 1068
                  PA   + A ++ AVC  T YPDSC SSIS+L+  NT DPEELFRL+LRV
Sbjct: 61   SV----PASPVSPATSIKAVCSVTQYPDSCVSSISSLDTSNTTDPEELFRLTLRV 111


>ref|XP_002304256.1| predicted protein [Populus trichocarpa] gi|222841688|gb|EEE79235.1|
            predicted protein [Populus trichocarpa]
          Length = 588

 Score =  465 bits (1196), Expect(2) = e-149
 Identities = 233/333 (69%), Positives = 273/333 (81%), Gaps = 11/333 (3%)
 Frame = -2

Query: 966  AFKVCQIVLDDALERLNDTLSSMDVNESKGQKLLSVTKLDDLKTWLSATITDQETCLDAL 787
            A KVC  V DDA++RLND++SSM + E  G+++LS  K++DLKTWLSATITDQETCLDAL
Sbjct: 140  ALKVCATVFDDAVDRLNDSISSMAIGE--GEQILSPAKMNDLKTWLSATITDQETCLDAL 197

Query: 786  DE------FNATAL--VGDSLDKPTQYASNSLSIVAKILGVLARFNVPVHRRLLGIGGHG 631
             E      FN T +  V  +++  T++ SNSL+IVAKILG+L+ F +P+HRRLLG     
Sbjct: 198  QELSTTKHFNQTLVDQVKTAMENSTEFVSNSLAIVAKILGLLSDFKIPIHRRLLGFERSH 257

Query: 630  ---FPEWVSSGDRRLLQEVRPKPDVTVAADGSGDVKTIREAAERIPKKSTKRFVIYVKAG 460
               FP+WVS GDRRLLQE +P P+VTVA DGSGD +T+REA  +IPKKS  +F+I+VK G
Sbjct: 258  ISEFPQWVSFGDRRLLQESKPTPNVTVAKDGSGDCETLREAVGKIPKKSESKFIIHVKEG 317

Query: 459  EYLGNNILDKSKWNVMMYGDGKGQTILSGSLNFIDGTPTFSTATFAAVGKGFIAKDMTFK 280
             Y+ N ILDKSKWNVM+YG+GK +TI+SGSLNF+DGTPTFST TFA  GKGF A+DM F 
Sbjct: 318  VYVENVILDKSKWNVMIYGEGKDKTIVSGSLNFVDGTPTFSTPTFAVAGKGFFARDMKFI 377

Query: 279  NTAGAEKHQAVAFRSGSDFSVFYRCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIFGNAA 100
            NTAGAEKHQAVAFRSGSD SVFYRC+FDA+QDTLYAHSNRQFYRDCDITGTIDFIFGNAA
Sbjct: 378  NTAGAEKHQAVAFRSGSDMSVFYRCAFDAFQDTLYAHSNRQFYRDCDITGTIDFIFGNAA 437

Query: 99   VVFQNCKIQPRQPMPNQFVTITAQGKKDPNQNT 1
            VVFQNC IQPRQP+ NQF TITAQGKKDPNQNT
Sbjct: 438  VVFQNCNIQPRQPLANQFNTITAQGKKDPNQNT 470



 Score = 92.4 bits (228), Expect(2) = e-149
 Identities = 55/113 (48%), Positives = 58/113 (51%)
 Frame = -3

Query: 1406 MDSIQSFKGYGKVDPVEEAAFRKKSRKRXXXXXXXXXXXXXXXXXXXXXXXVHXXXXXXX 1227
            MDSI SFKGYGKVD  E+ AFRKKSRKR                       VH       
Sbjct: 1    MDSINSFKGYGKVDEAEQQAFRKKSRKRLIIIVVSSIVLLAVIIGAVIGAVVHKRNNKSS 60

Query: 1226 XXXXSDPAQTSAAALNAVCKQTLYPDSCHSSISALNLKNTADPEELFRLSLRV 1068
                  P  T A +L AVC  T YP SC SSISAL   NT DPE LF+LSLRV
Sbjct: 61   SNPVPPPELTPATSLKAVCSVTQYPASCFSSISALETGNTTDPEVLFKLSLRV 113


>ref|XP_002532945.1| Pectinesterase-3 precursor, putative [Ricinus communis]
            gi|223527274|gb|EEF29429.1| Pectinesterase-3 precursor,
            putative [Ricinus communis]
          Length = 589

 Score =  457 bits (1177), Expect(2) = e-145
 Identities = 225/334 (67%), Positives = 266/334 (79%), Gaps = 10/334 (2%)
 Frame = -2

Query: 972  KEAFKVCQIVLDDALERLNDTLSSMDVNESKGQKLLSVTKLDDLKTWLSATITDQETCLD 793
            K A  VC+ V DDA + LND++SSM V E  G+ +LS +K++D+KTWLS TITDQETCLD
Sbjct: 139  KAALNVCESVFDDAFDMLNDSISSMTVGE--GETILSPSKINDMKTWLSTTITDQETCLD 196

Query: 792  ALDEFNATAL--------VGDSLDKPTQYASNSLSIVAKILGVLARFNVPVHRRLLGIG- 640
            AL E N T          +  +++  T++ASNSL+IVAKI+G+L  FN+P+HR+LLG   
Sbjct: 197  ALQELNGTKHFDSKVLEDIRTAMENSTEFASNSLAIVAKIIGLLTDFNIPIHRKLLGFER 256

Query: 639  -GHGFPEWVSSGDRRLLQEVRPKPDVTVAADGSGDVKTIREAAERIPKKSTKRFVIYVKA 463
               GFP WVS GDRRLLQE +P  +VTVA DGSGD  TI EA + IPKKS  RF+I+VK 
Sbjct: 257  TSSGFPSWVSIGDRRLLQEAKPAANVTVAKDGSGDYTTIGEAVDAIPKKSPSRFIIHVKE 316

Query: 462  GEYLGNNILDKSKWNVMMYGDGKGQTILSGSLNFIDGTPTFSTATFAAVGKGFIAKDMTF 283
            G Y+ N ++DK KWNVM+YGDGK +TI+SGS NF+DGTPTFSTATFA  GKGF+A+D+ F
Sbjct: 317  GTYVENILMDKHKWNVMIYGDGKDKTIISGSTNFVDGTPTFSTATFAVAGKGFMARDIKF 376

Query: 282  KNTAGAEKHQAVAFRSGSDFSVFYRCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIFGNA 103
             NTAGA KHQAVAFRSGSD SV+++CSFDAYQDTLYAHSNRQFYRDCDITGTIDFIFGNA
Sbjct: 377  INTAGAAKHQAVAFRSGSDMSVYFQCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIFGNA 436

Query: 102  AVVFQNCKIQPRQPMPNQFVTITAQGKKDPNQNT 1
            AVVFQNC I PRQP+PNQF TITAQGKKDPNQNT
Sbjct: 437  AVVFQNCNIMPRQPLPNQFNTITAQGKKDPNQNT 470



 Score = 84.7 bits (208), Expect(2) = e-145
 Identities = 48/113 (42%), Positives = 59/113 (52%)
 Frame = -3

Query: 1406 MDSIQSFKGYGKVDPVEEAAFRKKSRKRXXXXXXXXXXXXXXXXXXXXXXXVHXXXXXXX 1227
            MDSI SFKGYGKVD +E+ A+R+K+RKR                       +H       
Sbjct: 1    MDSINSFKGYGKVDELEQQAYRRKTRKRLIILIVSFIVLVAIIIGAVVGTVIHKRNNDSS 60

Query: 1226 XXXXSDPAQTSAAALNAVCKQTLYPDSCHSSISALNLKNTADPEELFRLSLRV 1068
                     T AA+L AVC  T +P SC SSIS+L   NT+DPE LF+LSL V
Sbjct: 61   PNSAVPTELTPAASLKAVCSVTQFPSSCFSSISSLETANTSDPEVLFKLSLHV 113


>gb|AFI23414.1| pectin methylesterase [Coffea arabica]
          Length = 582

 Score =  434 bits (1117), Expect(2) = e-137
 Identities = 220/332 (66%), Positives = 262/332 (78%), Gaps = 8/332 (2%)
 Frame = -2

Query: 972  KEAFKVCQIVLDDALERLNDTLSSMDVNESKGQKLLSVTKLDDLKTWLSATITDQETCLD 793
            K+A  VCQ V+DDA++  +++LSS++V+E  G +LL+V +++DLKTWLSA++TD ETCLD
Sbjct: 138  KKALGVCQAVIDDAVDATDESLSSLNVSE--GDRLLTVDRVNDLKTWLSASLTDLETCLD 195

Query: 792  ALDEFNATAL---VGDSLDKPTQYASNSLSIVAKILGVLARFNVPVHRRLLGIGGH---G 631
            +L E NAT L   V  S    T++ASNSL+IV+K+L +L+ FN+P+HR+LL  G     G
Sbjct: 196  SLQEVNATVLAEQVRASSRNSTEFASNSLAIVSKLLTILSGFNIPIHRKLLAAGTDSDGG 255

Query: 630  FPEWVSSGDRRLLQ--EVRPKPDVTVAADGSGDVKTIREAAERIPKKSTKRFVIYVKAGE 457
            FP WV + DRRLLQ      KPD+ VA DGSGD +TI EA  +IPKKS  RFVIYVKAG 
Sbjct: 256  FPRWVRAADRRLLQTPNENTKPDLVVAQDGSGDYRTISEAVAKIPKKSKTRFVIYVKAGV 315

Query: 456  YLGNNILDKSKWNVMMYGDGKGQTILSGSLNFIDGTPTFSTATFAAVGKGFIAKDMTFKN 277
            Y     LDKS WNVMMYGDGK +TI++   NF+DGTPTF TATFA  GKGFIAK M F+N
Sbjct: 316  YKEKVSLDKSTWNVMMYGDGKAKTIVTSDDNFVDGTPTFDTATFAVAGKGFIAKSMAFRN 375

Query: 276  TAGAEKHQAVAFRSGSDFSVFYRCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAV 97
            TAGA KHQAVAFRSGSD SV Y CSFDA+QDTLY HSNRQFYR+CDI+GTIDFIFGNAAV
Sbjct: 376  TAGAAKHQAVAFRSGSDQSVLYLCSFDAFQDTLYPHSNRQFYRECDISGTIDFIFGNAAV 435

Query: 96   VFQNCKIQPRQPMPNQFVTITAQGKKDPNQNT 1
            VFQNC I+PRQP+PNQFVTITAQGKKDPNQNT
Sbjct: 436  VFQNCNIRPRQPLPNQFVTITAQGKKDPNQNT 467



 Score = 82.8 bits (203), Expect(2) = e-137
 Identities = 47/114 (41%), Positives = 65/114 (57%), Gaps = 1/114 (0%)
 Frame = -3

Query: 1406 MDSIQSFKGYGKVDPVEEAAFRKKSRKRXXXXXXXXXXXXXXXXXXXXXXXVHXXXXXXX 1227
            MD+I+SFKGYGKVDPVEE AFR+K+R+R                        H       
Sbjct: 2    MDTIKSFKGYGKVDPVEEQAFRRKTRRRIIVLLVSLLLLVALIVGVVVATVAH--KKKNS 59

Query: 1226 XXXXSDPAQTSAAALNAVCKQTLYPDSCHSSISALNLKN-TADPEELFRLSLRV 1068
                +D   + + +L A+C  T++PDSC++SIS+L   N T DPE+LF+LSL+V
Sbjct: 60   KGNANDAPTSPSQSLRAICSVTVHPDSCYTSISSLEASNSTTDPEKLFQLSLQV 113


>ref|XP_004144445.1| PREDICTED: pectinesterase 3-like [Cucumis sativus]
            gi|449528768|ref|XP_004171375.1| PREDICTED:
            pectinesterase 3-like [Cucumis sativus]
          Length = 576

 Score =  435 bits (1119), Expect(2) = e-133
 Identities = 218/325 (67%), Positives = 258/325 (79%), Gaps = 3/325 (0%)
 Frame = -2

Query: 966  AFKVCQIVLDDALERLNDTLSSMDVNESKGQKLLSVTKLDDLKTWLSATITDQETCLDAL 787
            A K+C+ V +DA++ LNDT+SSM+V+    +K LS ++++DLKTWLS TITDQETCLDAL
Sbjct: 139  AIKICRSVFEDAIDTLNDTVSSMEVDRHS-EKFLSPSRIEDLKTWLSTTITDQETCLDAL 197

Query: 786  DEFNATALVGD---SLDKPTQYASNSLSIVAKILGVLARFNVPVHRRLLGIGGHGFPEWV 616
             + N T ++ D   ++   T++ SNSL+IV KILG+LA FN+P+HR+L+G+     PEWV
Sbjct: 198  RDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIPIHRKLMGL-----PEWV 252

Query: 615  SSGDRRLLQEVRPKPDVTVAADGSGDVKTIREAAERIPKKSTKRFVIYVKAGEYLGNNIL 436
            SSGDRRLLQE      VTV+ DG G   TI++A   +PKKS +RF+I+VK G Y  N IL
Sbjct: 253  SSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIHVKEGIYEENVIL 312

Query: 435  DKSKWNVMMYGDGKGQTILSGSLNFIDGTPTFSTATFAAVGKGFIAKDMTFKNTAGAEKH 256
            DKSKWNVMMYGDG+ +TI+SG LNFIDGTPTFSTATFA  GKGFI KDM F NTAG  KH
Sbjct: 313  DKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKDMGFINTAGPAKH 372

Query: 255  QAVAFRSGSDFSVFYRCSFDAYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNCKI 76
            QAVAFRSGSD SV   CSFD YQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNC I
Sbjct: 373  QAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNCNI 432

Query: 75   QPRQPMPNQFVTITAQGKKDPNQNT 1
            +PRQP+PNQF TITAQGKKD NQN+
Sbjct: 433  RPRQPLPNQFNTITAQGKKDINQNS 457



 Score = 68.2 bits (165), Expect(2) = e-133
 Identities = 45/115 (39%), Positives = 56/115 (48%), Gaps = 2/115 (1%)
 Frame = -3

Query: 1406 MDSIQSFKGYGKVDPVEEAAFRKKSRKRXXXXXXXXXXXXXXXXXXXXXXXVHXXXXXXX 1227
            MD+++SFKGYGKVD +E+ AFR+K+R+R                       VH       
Sbjct: 1    MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60

Query: 1226 XXXXSDPAQ--TSAAALNAVCKQTLYPDSCHSSISALNLKNTADPEELFRLSLRV 1068
                S P    T  A+L  +C  T YP SC SS   L   NT DP  LF+LSLRV
Sbjct: 61   STTTSPPPTELTPPASLKTLCSVTQYPSSCQSS---LQNSNTTDPVFLFKLSLRV 112