BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003123
         (1729 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase,...   904   0.0  
gb|AFP93564.1| GCS [Cestrum nocturnum]                                894   0.0  
ref|XP_002273626.1| PREDICTED: glutamate--cysteine ligase, chlor...   883   0.0  
ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast prec...   881   0.0  
dbj|BAD27390.1| gamma-glutamylcysteine synthetase [Zinnia elegans]    877   0.0  

>sp|Q1W2L8.2|GSH1_TOBAC RecName: Full=Glutamate--cysteine ligase, chloroplastic; AltName:
            Full=Gamma-ECS; Short=GCS; AltName:
            Full=Gamma-glutamylcysteine synthetase; Flags: Precursor
            gi|111380512|gb|ABD98695.2| chloroplast
            gamma-glutamylcysteine synthetase [Nicotiana tabacum]
          Length = 522

 Score =  904 bits (2337), Expect = 0.0
 Identities = 445/524 (84%), Positives = 472/524 (90%), Gaps = 1/524 (0%)
 Frame = -1

Query: 1618 MATLSM-GSSYCIPSESMQSKAGQNVYNIASYTEASTMKGHCIGFPSMTNPRNTMQKLRS 1442
            MA +S  GSS+CI SE M+  +G +  +I S  E   MK  C G  S  N    MQ +  
Sbjct: 1    MALMSQAGSSHCIYSEKMKCISGHS--SITSNMEMLKMKDICFGNISSRNSSKPMQGIYL 58

Query: 1441 DGIRVGRKRGQQVIVAASPPTEDAVIATEPLTREDLVAYLASGCKSKEKWRIGTEHEKFG 1262
            D + V R+RG+  IVAASPPTEDAV+A EPLT+EDLVAYLASGCKSKEKWRIGTEHEKFG
Sbjct: 59   DRVGVERRRGRLAIVAASPPTEDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKFG 118

Query: 1261 FESQTLRPMKYEQISELLYGISERFDWEKIMEGDYIIGLKQGKQSISLEPGGQFELSGAP 1082
            FE  TLRPMKYEQI+ELL GI+ERFDWEK+MEGD IIGLKQGKQSISLEPGGQFELSGAP
Sbjct: 119  FEFGTLRPMKYEQIAELLNGIAERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFELSGAP 178

Query: 1081 LETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWAIKDIPIMPKGRYEIMRNYMPK 902
            LETLHQTCAEVNSHLYQVKAVAEEMGIGFLG GFQPKW +KDIP+MPKGRYEIMRNYMPK
Sbjct: 179  LETLHQTCAEVNSHLYQVKAVAEEMGIGFLGTGFQPKWGLKDIPVMPKGRYEIMRNYMPK 238

Query: 901  VGTLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLS 722
            VG+LGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLS
Sbjct: 239  VGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLS 298

Query: 721  MRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDFL 542
            MRS IWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAG+SFRDF+
Sbjct: 299  MRSHIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGMSFRDFM 358

Query: 541  AGKLSPIPGEYPNLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYD 362
             GKLSPIPG+YP LNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYD
Sbjct: 359  NGKLSPIPGDYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYD 418

Query: 361  EVSLQKVLDMIADWTMEEREMLRNKVPVSGLKTPFRDGLLKHIAQDVMQLAKDGLERRGY 182
            EVSLQ VLDM +DWT EEREMLRNKVP SGLKTPFRDGLLKH+AQDV++LAK+GLERRGY
Sbjct: 419  EVSLQTVLDMTSDWTAEEREMLRNKVPTSGLKTPFRDGLLKHVAQDVVKLAKEGLERRGY 478

Query: 181  KETGFLNEVAEVARTGVTPAEKLLELYHGKWGESVDPVFQELLY 50
            KETGFLNEV EV RTGVTPAEKLLELYHGKWG SVDPVF+ELLY
Sbjct: 479  KETGFLNEVTEVVRTGVTPAEKLLELYHGKWGRSVDPVFEELLY 522


>gb|AFP93564.1| GCS [Cestrum nocturnum]
          Length = 518

 Score =  894 bits (2311), Expect = 0.0
 Identities = 441/525 (84%), Positives = 467/525 (88%), Gaps = 2/525 (0%)
 Frame = -1

Query: 1618 MATLSM-GSSYCIPSESMQSKAGQNVYNIASYTEASTMKGHCIGFP-SMTNPRNTMQKLR 1445
            MA +S  GSS+CI  E ++ K G N+       E   +K  C G   S  N  N  Q + 
Sbjct: 1    MALMSQAGSSHCIHPEKIRCKGGHNM-------EIFKLKEICFGVHISPRNSSNAKQGIC 53

Query: 1444 SDGIRVGRKRGQQVIVAASPPTEDAVIATEPLTREDLVAYLASGCKSKEKWRIGTEHEKF 1265
               +   R+RG+  IVAASPPT+DAV+A EPLT+EDLVAYLASGCKSKEKWRIGTEHEKF
Sbjct: 54   LRRVGAERRRGRLSIVAASPPTDDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKF 113

Query: 1264 GFESQTLRPMKYEQISELLYGISERFDWEKIMEGDYIIGLKQGKQSISLEPGGQFELSGA 1085
            GFE  TLRPM YEQI+ELLYG+SERFDWEK+MEGD IIGLKQGKQSISLEPGGQFELSGA
Sbjct: 114  GFEFGTLRPMNYEQIAELLYGVSERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFELSGA 173

Query: 1084 PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWAIKDIPIMPKGRYEIMRNYMP 905
            PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKW +KDIP+MPKGRYEIMRNYMP
Sbjct: 174  PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWGLKDIPVMPKGRYEIMRNYMP 233

Query: 904  KVGTLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYL 725
            KVGTLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPF EGKPNGYL
Sbjct: 234  KVGTLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFKEGKPNGYL 293

Query: 724  SMRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDF 545
            SMRS IWTDTDNNR+GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDF
Sbjct: 294  SMRSHIWTDTDNNRSGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDF 353

Query: 544  LAGKLSPIPGEYPNLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILY 365
            L GKL+PIPGEYP LNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILY
Sbjct: 354  LNGKLAPIPGEYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILY 413

Query: 364  DEVSLQKVLDMIADWTMEEREMLRNKVPVSGLKTPFRDGLLKHIAQDVMQLAKDGLERRG 185
            DE SLQ VLDM ADWT EEREMLRNKVP SGLKTPFRDGLLKH+AQDVM+LAK+GLERRG
Sbjct: 414  DEASLQSVLDMTADWTAEEREMLRNKVPKSGLKTPFRDGLLKHVAQDVMKLAKEGLERRG 473

Query: 184  YKETGFLNEVAEVARTGVTPAEKLLELYHGKWGESVDPVFQELLY 50
            YKETGFLNEV EV RTGVTPAEKLL+LYHGKWG+SVDPVF+ELLY
Sbjct: 474  YKETGFLNEVTEVVRTGVTPAEKLLDLYHGKWGQSVDPVFEELLY 518


>ref|XP_002273626.1| PREDICTED: glutamate--cysteine ligase, chloroplastic [Vitis vinifera]
            gi|297742523|emb|CBI34672.3| unnamed protein product
            [Vitis vinifera]
          Length = 526

 Score =  883 bits (2282), Expect = 0.0
 Identities = 431/519 (83%), Positives = 464/519 (89%), Gaps = 2/519 (0%)
 Frame = -1

Query: 1600 GSSYCIPSESMQSKAGQN-VYNIASYTEASTMKGHCIGFPSMTNPRN-TMQKLRSDGIRV 1427
            G SYC+ SE +  K GQ+ V N+ +  EAS +K       S++  R  T   L  D + V
Sbjct: 8    GPSYCMRSEVVHCKTGQSAVVNMTNNMEASKIKETRTWLSSLSRSRTKTKHILSPDSVGV 67

Query: 1426 GRKRGQQVIVAASPPTEDAVIATEPLTREDLVAYLASGCKSKEKWRIGTEHEKFGFESQT 1247
            G K+  QVIVAASPPTEDAV+ATEPLTREDL+ YLASGCK KEKWRIGTEHEKFGFE +T
Sbjct: 68   GCKQRHQVIVAASPPTEDAVVATEPLTREDLIGYLASGCKPKEKWRIGTEHEKFGFEFRT 127

Query: 1246 LRPMKYEQISELLYGISERFDWEKIMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLH 1067
            LRPM+YEQISELLYG+SERF W+KIMEGD IIGLKQGKQSISLEPGGQFELSGAPLETLH
Sbjct: 128  LRPMRYEQISELLYGVSERFGWDKIMEGDNIIGLKQGKQSISLEPGGQFELSGAPLETLH 187

Query: 1066 QTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWAIKDIPIMPKGRYEIMRNYMPKVGTLG 887
            QTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWAIKDIPIMPKGRYEIMRNYMPKVG+LG
Sbjct: 188  QTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWAIKDIPIMPKGRYEIMRNYMPKVGSLG 247

Query: 886  LDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQI 707
            LDMMFRTCTVQVNLDFSSE DMIRKFR GLALQPIATALFANSPF EGKP+GYLSMRSQI
Sbjct: 248  LDMMFRTCTVQVNLDFSSETDMIRKFRVGLALQPIATALFANSPFVEGKPSGYLSMRSQI 307

Query: 706  WTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDFLAGKLS 527
            WTDTDNNR GMLPFVFD SFGFEQYVDYALDVPMYFVYRKKKYIDC G+SFRDF+AGKL 
Sbjct: 308  WTDTDNNRTGMLPFVFDGSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMSFRDFIAGKLP 367

Query: 526  PIPGEYPNLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQ 347
             +PGE PN NDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQ
Sbjct: 368  SLPGELPNFNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQ 427

Query: 346  KVLDMIADWTMEEREMLRNKVPVSGLKTPFRDGLLKHIAQDVMQLAKDGLERRGYKETGF 167
              LD+IADWT+EER+MLRNKVP +GLKTPFRDGLLKH+A+DV++LAKDGLERRG+KETGF
Sbjct: 428  NALDIIADWTLEERQMLRNKVPKTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGFKETGF 487

Query: 166  LNEVAEVARTGVTPAEKLLELYHGKWGESVDPVFQELLY 50
            LN VAEV RTGVTPAEKLLE+YHG WG+ VDPVF+ELLY
Sbjct: 488  LNAVAEVVRTGVTPAEKLLEMYHGPWGQCVDPVFEELLY 526


>ref|XP_002509800.1| Glutamate--cysteine ligase, chloroplast precursor, putative [Ricinus
            communis] gi|223549699|gb|EEF51187.1| Glutamate--cysteine
            ligase, chloroplast precursor, putative [Ricinus
            communis]
          Length = 526

 Score =  881 bits (2276), Expect = 0.0
 Identities = 431/519 (83%), Positives = 463/519 (89%), Gaps = 2/519 (0%)
 Frame = -1

Query: 1600 GSSYCIPSESMQSKAGQN-VYNIASYTEASTMKGHCIGFPSMT-NPRNTMQKLRSDGIRV 1427
            G S  +P++  + K GQN V  + +  E S +K  C+ F S++ N     Q   S+   V
Sbjct: 8    GPSCYVPTDITRCKTGQNAVLGVFNSIETSKLKETCVRFSSLSCNSTKIPQLPSSENSWV 67

Query: 1426 GRKRGQQVIVAASPPTEDAVIATEPLTREDLVAYLASGCKSKEKWRIGTEHEKFGFESQT 1247
              +RG Q+IVAASPP EDAVIATEPLT+EDLV YLASGCK KEKWRIGTEHEKFGFE  T
Sbjct: 68   RSRRGNQMIVAASPPIEDAVIATEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFELGT 127

Query: 1246 LRPMKYEQISELLYGISERFDWEKIMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLH 1067
            LRPMKYEQI+ELL GI+ERFDWEK+MEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLH
Sbjct: 128  LRPMKYEQIAELLNGIAERFDWEKVMEGDYIIGLKQGKQSISLEPGGQFELSGAPLETLH 187

Query: 1066 QTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWAIKDIPIMPKGRYEIMRNYMPKVGTLG 887
            QTCAEVNSHLYQVKAV EEMGIGFLGIGFQPKW +KDIPIMPKGRYEIMRNYMPKVGTLG
Sbjct: 188  QTCAEVNSHLYQVKAVTEEMGIGFLGIGFQPKWGVKDIPIMPKGRYEIMRNYMPKVGTLG 247

Query: 886  LDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQI 707
            LDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQI
Sbjct: 248  LDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYLSMRSQI 307

Query: 706  WTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDFLAGKLS 527
            WTDTD +R GMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDC G++FRDFLAGKL 
Sbjct: 308  WTDTDKDRTGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCTGMTFRDFLAGKLP 367

Query: 526  PIPGEYPNLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILYDEVSLQ 347
             IPGE P LNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LYDE+SLQ
Sbjct: 368  CIPGELPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGLLYDEISLQ 427

Query: 346  KVLDMIADWTMEEREMLRNKVPVSGLKTPFRDGLLKHIAQDVMQLAKDGLERRGYKETGF 167
             VLDM ADWT EEREMLRNKVP +GLKTPFRDGLLKH+A+DV++LAKDGLERRGYKE GF
Sbjct: 428  NVLDMTADWTPEEREMLRNKVPKTGLKTPFRDGLLKHVAEDVLKLAKDGLERRGYKEIGF 487

Query: 166  LNEVAEVARTGVTPAEKLLELYHGKWGESVDPVFQELLY 50
            LNEV EV RTGVTPAE+LLELY+GKWG+SVDPVF+ELLY
Sbjct: 488  LNEVTEVVRTGVTPAERLLELYNGKWGQSVDPVFEELLY 526


>dbj|BAD27390.1| gamma-glutamylcysteine synthetase [Zinnia elegans]
          Length = 523

 Score =  877 bits (2265), Expect = 0.0
 Identities = 432/525 (82%), Positives = 468/525 (89%), Gaps = 2/525 (0%)
 Frame = -1

Query: 1618 MATLSMGS-SYCIPSESMQSKAG-QNVYNIASYTEASTMKGHCIGFPSMTNPRNTMQKLR 1445
            M  +S  S S+ I +E +QSK+G  ++ N AS T A   +   + F    N     Q + 
Sbjct: 1    MVLMSQASPSHGIHAEILQSKSGYSSLLNGASNTNAFRHQTSKVAFSR--NYLKYTQAMH 58

Query: 1444 SDGIRVGRKRGQQVIVAASPPTEDAVIATEPLTREDLVAYLASGCKSKEKWRIGTEHEKF 1265
             D +    KRG +VIVAASPPTEDAV+ATEPLT+EDLV YLASGCK KE WRIGTEHEKF
Sbjct: 59   VDAVGGNFKRGNKVIVAASPPTEDAVVATEPLTKEDLVGYLASGCKPKENWRIGTEHEKF 118

Query: 1264 GFESQTLRPMKYEQISELLYGISERFDWEKIMEGDYIIGLKQGKQSISLEPGGQFELSGA 1085
            GF+ +TLRPM YEQI+ LL  ISERFDWEK+MEGD IIGLKQGKQSISLEPGGQFELSGA
Sbjct: 119  GFDLKTLRPMTYEQIAHLLNAISERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFELSGA 178

Query: 1084 PLETLHQTCAEVNSHLYQVKAVAEEMGIGFLGIGFQPKWAIKDIPIMPKGRYEIMRNYMP 905
            PLETLHQTCAEVNSHLYQVKAVAEEMGIGF+GIGFQPKW  KDIPIMPKGRYEIMRNYMP
Sbjct: 179  PLETLHQTCAEVNSHLYQVKAVAEEMGIGFIGIGFQPKWERKDIPIMPKGRYEIMRNYMP 238

Query: 904  KVGTLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYL 725
            KVG+LGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYL
Sbjct: 239  KVGSLGLDMMFRTCTVQVNLDFSSEADMIRKFRAGLALQPIATALFANSPFTEGKPNGYL 298

Query: 724  SMRSQIWTDTDNNRAGMLPFVFDDSFGFEQYVDYALDVPMYFVYRKKKYIDCAGLSFRDF 545
            SMRSQIWTDTDN+R+GMLPFVFDDSFGFEQYV+YALDVPMYFVYRKKKYIDCAGLSFRDF
Sbjct: 299  SMRSQIWTDTDNDRSGMLPFVFDDSFGFEQYVEYALDVPMYFVYRKKKYIDCAGLSFRDF 358

Query: 544  LAGKLSPIPGEYPNLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGILY 365
            LAGKL PIPGEYP LNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVG+LY
Sbjct: 359  LAGKLPPIPGEYPTLNDWENHLTTIFPEVRLKRYLEMRGADGGPWRRLCALPAFWVGVLY 418

Query: 364  DEVSLQKVLDMIADWTMEEREMLRNKVPVSGLKTPFRDGLLKHIAQDVMQLAKDGLERRG 185
            D++SLQ VLDM ADWT EER+MLRNKVPV+GLKTPFRDGLLKH+A++V++ AKDGLERRG
Sbjct: 419  DDISLQNVLDMTADWTQEERQMLRNKVPVAGLKTPFRDGLLKHVAEEVLKFAKDGLERRG 478

Query: 184  YKETGFLNEVAEVARTGVTPAEKLLELYHGKWGESVDPVFQELLY 50
            YKETGFLNEVAEV RTG+TPAEKLLELYHGKWG+SVDPVF+ELLY
Sbjct: 479  YKETGFLNEVAEVVRTGLTPAEKLLELYHGKWGQSVDPVFEELLY 523