BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003063
         (1219 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAK61826.1| coclaurine N-methyltransferase [Citrus unshiu]        590   e-166
ref|XP_002324742.1| predicted protein [Populus trichocarpa] gi|2...   587   e-165
ref|XP_002280292.1| PREDICTED: (S)-coclaurine N-methyltransferas...   581   e-163
gb|AFK34952.1| unknown [Medicago truncatula] gi|388496886|gb|AFK...   575   e-161
ref|XP_003603179.1| Cyclopropane-fatty-acyl-phospholipid synthas...   573   e-161

>dbj|BAK61826.1| coclaurine N-methyltransferase [Citrus unshiu]
          Length = 357

 Score =  590 bits (1520), Expect = e-166
 Identities = 275/354 (77%), Positives = 312/354 (88%)
 Frame = -2

Query: 1149 MKAIMEVPYAATVRMMLASLERNMLPDAVIXXXXXXXXXXXXXLGYKSSAELQLAQLVEF 970
            M+ IM   Y ATVR+ML+SLERN+LPDAVI              GYK SAELQL+ L++F
Sbjct: 1    MEGIMHAAYDATVRLMLSSLERNLLPDAVIRRLSRLLLGGRLRSGYKPSAELQLSDLLQF 60

Query: 969  AHALKDMPIAINTDDAKVQHYELPTSFFKLVLGENLKYSCCYFNDTSSTLDDAEKAMLEI 790
            AH+L++MPIAI TD AK QHYELPTSFFKLVLG+  KYSCCYF+D S TL+DAEKAMLE+
Sbjct: 61   AHSLREMPIAIQTDKAKEQHYELPTSFFKLVLGKYFKYSCCYFSDASKTLEDAEKAMLEL 120

Query: 789  YCERSGIEDGHTVLDVGCGWGSLSIYIAKKYRNCKVTGICNSTTQKAFIEEQCRDLKIYN 610
            YCERS +EDGHTVLDVGCGWGSLS+YIA+KY NCK+TGICNS TQK FIEEQCR L++ N
Sbjct: 121  YCERSRLEDGHTVLDVGCGWGSLSLYIAQKYSNCKITGICNSKTQKEFIEEQCRVLELQN 180

Query: 609  VEIIVADISTFDMEASYDRIFSIEMFEHMKNYKKLLKKISQWMKPDGLLFVHHFCHKTFA 430
            VEIIVADISTF+MEASYDRI+SIEMFEHMKNY+ LLKKIS+WMK D LLFVHHFCHKTFA
Sbjct: 181  VEIIVADISTFEMEASYDRIYSIEMFEHMKNYQNLLKKISKWMKEDTLLFVHHFCHKTFA 240

Query: 429  YHFEDINEDDWITRYFFSGGTMPSANLLLYFQDDVSVVNHWLVNGKHYAQTSEEWLKKMD 250
            YHFED N+DDWIT+YFF+GGTMPSANLLLYFQDDVSVV+HWLVNGKHYAQTSEEWLK+MD
Sbjct: 241  YHFEDTNDDDWITKYFFTGGTMPSANLLLYFQDDVSVVDHWLVNGKHYAQTSEEWLKRMD 300

Query: 249  KSMSSIRPIMESTYGKDLVVKWTVYWRTFFISVAELFGYNNGDEWMVAHFLFKR 88
             +++SI+PIMESTYGKD  VKWTVYWRTFFI+VAELFGYNNG+EWMV HFLF++
Sbjct: 301  NNLASIKPIMESTYGKDQAVKWTVYWRTFFIAVAELFGYNNGEEWMVTHFLFRK 354


>ref|XP_002324742.1| predicted protein [Populus trichocarpa] gi|222866176|gb|EEF03307.1|
            predicted protein [Populus trichocarpa]
          Length = 355

 Score =  587 bits (1513), Expect = e-165
 Identities = 270/354 (76%), Positives = 316/354 (89%)
 Frame = -2

Query: 1149 MKAIMEVPYAATVRMMLASLERNMLPDAVIXXXXXXXXXXXXXLGYKSSAELQLAQLVEF 970
            M+ +M+VPY ATV++MLASLERN+LPDAVI               YK+S+ELQLA L++F
Sbjct: 1    MERLMQVPYEATVKVMLASLERNLLPDAVIRRLTRMLLADRLRSCYKTSSELQLADLLQF 60

Query: 969  AHALKDMPIAINTDDAKVQHYELPTSFFKLVLGENLKYSCCYFNDTSSTLDDAEKAMLEI 790
             H+LK+MPIAI TD  K QHYELPTSFFKLVLG+NLKYSCCYF+D S+TL+DAEKAMLE+
Sbjct: 61   VHSLKEMPIAIKTDKPKTQHYELPTSFFKLVLGKNLKYSCCYFSDKSNTLEDAEKAMLEL 120

Query: 789  YCERSGIEDGHTVLDVGCGWGSLSIYIAKKYRNCKVTGICNSTTQKAFIEEQCRDLKIYN 610
            YCERS ++DGHTVLDVGCGWGSLS+YIA+KY NCK+TGICNSTTQK  I+EQCRDL++ N
Sbjct: 121  YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYSNCKITGICNSTTQKVHIDEQCRDLQLQN 180

Query: 609  VEIIVADISTFDMEASYDRIFSIEMFEHMKNYKKLLKKISQWMKPDGLLFVHHFCHKTFA 430
            VEIIVA+ISTF+M+ASYDRI+SIEMFEHMKNY  LL KIS+WMK DGL FVH+FCHK FA
Sbjct: 181  VEIIVANISTFEMQASYDRIYSIEMFEHMKNYGDLLNKISKWMKQDGLHFVHYFCHKQFA 240

Query: 429  YHFEDINEDDWITRYFFSGGTMPSANLLLYFQDDVSVVNHWLVNGKHYAQTSEEWLKKMD 250
            YHFED+NEDDWITRYFF+GGTMPSANLLLYFQDDVS+V+HWLVNGKHY+QTSEEWLK+MD
Sbjct: 241  YHFEDVNEDDWITRYFFTGGTMPSANLLLYFQDDVSIVDHWLVNGKHYSQTSEEWLKRMD 300

Query: 249  KSMSSIRPIMESTYGKDLVVKWTVYWRTFFISVAELFGYNNGDEWMVAHFLFKR 88
            +++++I+PIMESTYGKD  VKWTVYWRTFFI+VAELFGYNNG+EWMVAHFLF +
Sbjct: 301  RNLAAIKPIMESTYGKDQAVKWTVYWRTFFIAVAELFGYNNGEEWMVAHFLFNK 354


>ref|XP_002280292.1| PREDICTED: (S)-coclaurine N-methyltransferase [Vitis vinifera]
            gi|296081677|emb|CBI20682.3| unnamed protein product
            [Vitis vinifera]
          Length = 355

 Score =  581 bits (1498), Expect = e-163
 Identities = 270/354 (76%), Positives = 314/354 (88%)
 Frame = -2

Query: 1149 MKAIMEVPYAATVRMMLASLERNMLPDAVIXXXXXXXXXXXXXLGYKSSAELQLAQLVEF 970
            M+ +M+VPY ATVR+MLASLERN+LPDA++              GYK SAELQL+ L+ F
Sbjct: 1    MEGMMQVPYDATVRLMLASLERNLLPDALVRRLTRLLLASRLRSGYKPSAELQLSSLLHF 60

Query: 969  AHALKDMPIAINTDDAKVQHYELPTSFFKLVLGENLKYSCCYFNDTSSTLDDAEKAMLEI 790
            AH+L++MPIAI T+D K QHYELPTSFFKLVLG+NLKYSCCYF D SSTL+DAE AMLE+
Sbjct: 61   AHSLEEMPIAIKTEDPKSQHYELPTSFFKLVLGKNLKYSCCYFPDKSSTLEDAENAMLEM 120

Query: 789  YCERSGIEDGHTVLDVGCGWGSLSIYIAKKYRNCKVTGICNSTTQKAFIEEQCRDLKIYN 610
            YC+R+ +EDGHTVLD+GCGWGSLS+YIA+KY NC++TGICNS TQKA+IEEQC++L++ N
Sbjct: 121  YCKRAQVEDGHTVLDIGCGWGSLSLYIAQKYTNCRITGICNSKTQKAYIEEQCQELQLQN 180

Query: 609  VEIIVADISTFDMEASYDRIFSIEMFEHMKNYKKLLKKISQWMKPDGLLFVHHFCHKTFA 430
            VEIIVADIST +MEA+YDRIFSIEMFEHMKNYK LLKKIS WMK D LLFVHHFCHK FA
Sbjct: 181  VEIIVADISTLEMEATYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHHFCHKAFA 240

Query: 429  YHFEDINEDDWITRYFFSGGTMPSANLLLYFQDDVSVVNHWLVNGKHYAQTSEEWLKKMD 250
            YHFED ++DDWITRYFFSGGTMP+ANLLLYFQ+DVSVV+HWLVNGKHYAQTSEEWLK+MD
Sbjct: 241  YHFEDKSDDDWITRYFFSGGTMPAANLLLYFQEDVSVVDHWLVNGKHYAQTSEEWLKRMD 300

Query: 249  KSMSSIRPIMESTYGKDLVVKWTVYWRTFFISVAELFGYNNGDEWMVAHFLFKR 88
            K+++SI+PIMESTYGKD  VKWTVYWRTFFISVAELF YN+G+EWMVA FLFK+
Sbjct: 301  KNLASIKPIMESTYGKDSAVKWTVYWRTFFISVAELFEYNDGEEWMVALFLFKK 354


>gb|AFK34952.1| unknown [Medicago truncatula] gi|388496886|gb|AFK36509.1| unknown
            [Medicago truncatula]
          Length = 358

 Score =  575 bits (1481), Expect = e-161
 Identities = 266/355 (74%), Positives = 309/355 (87%)
 Frame = -2

Query: 1152 KMKAIMEVPYAATVRMMLASLERNMLPDAVIXXXXXXXXXXXXXLGYKSSAELQLAQLVE 973
            KM+ +M +PY  TV++ML SLERN+LPD VI               YK S+ELQL+ L++
Sbjct: 3    KMEEMMHLPYNTTVKLMLGSLERNLLPDTVIRRLTRLLLSTRLRSSYKPSSELQLSDLLQ 62

Query: 972  FAHALKDMPIAINTDDAKVQHYELPTSFFKLVLGENLKYSCCYFNDTSSTLDDAEKAMLE 793
            FAH+L+DMPIA++TD  K QHYELPTSFFKLVLG NLKYS CYF+  S TL+DAE+ ML+
Sbjct: 63   FAHSLQDMPIAVSTDVPKSQHYELPTSFFKLVLGNNLKYSSCYFSSASKTLEDAEEEMLK 122

Query: 792  IYCERSGIEDGHTVLDVGCGWGSLSIYIAKKYRNCKVTGICNSTTQKAFIEEQCRDLKIY 613
            +YCERS ++DGHTVLDVGCGWGSL+++IAK Y + +VTGICNSTTQKAFIEE+CR+L++ 
Sbjct: 123  LYCERSNLKDGHTVLDVGCGWGSLTLFIAKNYSSSRVTGICNSTTQKAFIEEKCRELQLQ 182

Query: 612  NVEIIVADISTFDMEASYDRIFSIEMFEHMKNYKKLLKKISQWMKPDGLLFVHHFCHKTF 433
            NV+IIVADISTF+MEASYDRIFSIEMFEHMKNYK LLKKIS WMK DGLLFVHHFCHK F
Sbjct: 183  NVDIIVADISTFEMEASYDRIFSIEMFEHMKNYKDLLKKISIWMKEDGLLFVHHFCHKAF 242

Query: 432  AYHFEDINEDDWITRYFFSGGTMPSANLLLYFQDDVSVVNHWLVNGKHYAQTSEEWLKKM 253
            AYHFED NEDDWITRYFF+GGTMP+ANLLLYFQDDV+V+NHWLVNGKHYAQTSEEWLK+M
Sbjct: 243  AYHFEDKNEDDWITRYFFTGGTMPAANLLLYFQDDVTVINHWLVNGKHYAQTSEEWLKRM 302

Query: 252  DKSMSSIRPIMESTYGKDLVVKWTVYWRTFFISVAELFGYNNGDEWMVAHFLFKR 88
            DK+ +SI+PIMESTYGKD   KWTVYWRTFFI+VAELFGYNNG+EWMVAHFLFK+
Sbjct: 303  DKNKTSIKPIMESTYGKDSATKWTVYWRTFFIAVAELFGYNNGEEWMVAHFLFKK 357


>ref|XP_003603179.1| Cyclopropane-fatty-acyl-phospholipid synthase [Medicago truncatula]
            gi|355492227|gb|AES73430.1|
            Cyclopropane-fatty-acyl-phospholipid synthase [Medicago
            truncatula]
          Length = 355

 Score =  573 bits (1476), Expect = e-161
 Identities = 265/354 (74%), Positives = 308/354 (87%)
 Frame = -2

Query: 1149 MKAIMEVPYAATVRMMLASLERNMLPDAVIXXXXXXXXXXXXXLGYKSSAELQLAQLVEF 970
            M+ +M +PY  TV++ML SLERN+LPD VI               YK S+ELQL+ L++F
Sbjct: 1    MEEMMHLPYNTTVKLMLGSLERNLLPDTVIRRLTRLLLSTRLRSSYKPSSELQLSDLLQF 60

Query: 969  AHALKDMPIAINTDDAKVQHYELPTSFFKLVLGENLKYSCCYFNDTSSTLDDAEKAMLEI 790
            AH+L+DMPIA++TD  K QHYELPTSFFKLVLG NLKYS CYF+  S TL+DAE+ ML++
Sbjct: 61   AHSLQDMPIAVSTDVPKSQHYELPTSFFKLVLGNNLKYSSCYFSSASKTLEDAEEEMLKL 120

Query: 789  YCERSGIEDGHTVLDVGCGWGSLSIYIAKKYRNCKVTGICNSTTQKAFIEEQCRDLKIYN 610
            YCERS ++DGHTVLDVGCGWGSL+++IAK Y + +VTGICNSTTQKAFIEE+CR+L++ N
Sbjct: 121  YCERSNLKDGHTVLDVGCGWGSLTLFIAKNYSSSRVTGICNSTTQKAFIEEKCRELQLQN 180

Query: 609  VEIIVADISTFDMEASYDRIFSIEMFEHMKNYKKLLKKISQWMKPDGLLFVHHFCHKTFA 430
            V+IIVADISTF+MEASYDRIFSIEMFEHMKNYK LLKKIS WMK DGLLFVHHFCHK FA
Sbjct: 181  VDIIVADISTFEMEASYDRIFSIEMFEHMKNYKDLLKKISIWMKEDGLLFVHHFCHKAFA 240

Query: 429  YHFEDINEDDWITRYFFSGGTMPSANLLLYFQDDVSVVNHWLVNGKHYAQTSEEWLKKMD 250
            YHFED NEDDWITRYFF+GGTMP+ANLLLYFQDDV+V+NHWLVNGKHYAQTSEEWLK+MD
Sbjct: 241  YHFEDKNEDDWITRYFFTGGTMPAANLLLYFQDDVTVINHWLVNGKHYAQTSEEWLKRMD 300

Query: 249  KSMSSIRPIMESTYGKDLVVKWTVYWRTFFISVAELFGYNNGDEWMVAHFLFKR 88
            K+ +SI+PIMESTYGKD   KWTVYWRTFFI+VAELFGYNNG+EWMVAHFLFK+
Sbjct: 301  KNKTSIKPIMESTYGKDSATKWTVYWRTFFIAVAELFGYNNGEEWMVAHFLFKK 354