BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00003060
         (833 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAA96528.1| G protein beta-subunit-like protein [Nicotiana p...   313   4e-83
gb|ABB02625.1| G protein beta subunit-like protein [Solanum tube...   309   4e-82
ref|NP_001233881.1| ArcA2 protein [Solanum lycopersicum] gi|4589...   308   7e-82
ref|NP_001241455.1| uncharacterized protein LOC100778205 [Glycin...   308   1e-81
ref|XP_002281279.1| PREDICTED: guanine nucleotide-binding protei...   308   1e-81

>emb|CAA96528.1| G protein beta-subunit-like protein [Nicotiana plumbaginifolia]
          Length = 328

 Score =  313 bits (801), Expect = 4e-83
 Identities = 150/174 (86%), Positives = 161/174 (92%)
 Frame = -1

Query: 833 VRFSPNNVQPTIVSASWDKTVKIWNLNNCKLRSTLAGHNGYVNTVAVSPDGSLCASGGRD 654
           VRFSPN +QPTIVS SWD+TVKIWNL NCKLRSTLAGH GYVNTVAVSPDGSLCASGG+D
Sbjct: 156 VRFSPNTLQPTIVSGSWDRTVKIWNLTNCKLRSTLAGHAGYVNTVAVSPDGSLCASGGKD 215

Query: 653 GVILLWDLAEGKKLYSLDATSIIHALCFSPNRYWLCAATESSIKIWDLESKSIVVDLKVD 474
           G ILLWDLAEGKKLYSLDA SIIHALCFSPNRYWLCAATE+SIKIWDLESKSIVVDLKVD
Sbjct: 216 GTILLWDLAEGKKLYSLDAGSIIHALCFSPNRYWLCAATETSIKIWDLESKSIVVDLKVD 275

Query: 473 LKTEADMITEGTTAQTNAGKNKVIYCTSLSWSADGSTLFSGYTDGVVRVWGIGR 312
           LK E++M TEGTT   +A KNK++YCT LSWSADGSTLFSGYTDG++RVWGIGR
Sbjct: 276 LKQESEMATEGTTG--SACKNKIMYCTCLSWSADGSTLFSGYTDGLIRVWGIGR 327



 Score = 57.0 bits (136), Expect = 5e-06
 Identities = 54/204 (26%), Positives = 87/204 (42%), Gaps = 31/204 (15%)
 Frame = -1

Query: 824 SPNNVQPTIVSASWDKTVKIWNLNNCKL-----RSTLAGHNGYVNTVAVSPDGSLCASGG 660
           +P +    IV++S DK++ +W+L          R  L GH  +V  V +S DG    SG 
Sbjct: 24  TPIDNSDMIVTSSRDKSLIVWSLTKDGPQYGVPRRRLTGHGHFVQDVVLSSDGMFALSGS 83

Query: 659 RDGVILLWDLAEGKKLYS-LDATSIIHALCFS-PNRYWLCAATESSIKIWDL--ESKSIV 492
            DG + LWDL  G      +  T  + ++ FS  NR  + A+ + SIK+W+   E K  +
Sbjct: 84  WDGELRLWDLQAGTTARRFVGHTKDVLSVAFSVDNRQIVSASRDKSIKLWNTLGECKYTI 143

Query: 491 VD----------LKVDLKTEADMITEGTTAQ-------TNAGKNKVI-----YCTSLSWS 378
            +          ++    T    I  G+  +       TN      +     Y  +++ S
Sbjct: 144 QEGDSHSDWVSCVRFSPNTLQPTIVSGSWDRTVKIWNLTNCKLRSTLAGHAGYVNTVAVS 203

Query: 377 ADGSTLFSGYTDGVVRVWGIGR*K 306
            DGS   SG  DG + +W +   K
Sbjct: 204 PDGSLCASGGKDGTILLWDLAEGK 227


>gb|ABB02625.1| G protein beta subunit-like protein [Solanum tuberosum]
          Length = 261

 Score =  309 bits (792), Expect = 4e-82
 Identities = 148/174 (85%), Positives = 159/174 (91%)
 Frame = -1

Query: 833 VRFSPNNVQPTIVSASWDKTVKIWNLNNCKLRSTLAGHNGYVNTVAVSPDGSLCASGGRD 654
           VRFSPNN+QPTIVS SWD+TVKIWNL+NCKLR+TLAGH GYVNT AVSPDGSLCASGG+D
Sbjct: 88  VRFSPNNLQPTIVSGSWDRTVKIWNLSNCKLRATLAGHTGYVNTAAVSPDGSLCASGGKD 147

Query: 653 GVILLWDLAEGKKLYSLDATSIIHALCFSPNRYWLCAATESSIKIWDLESKSIVVDLKVD 474
           GVILLWDLAEGKKLYSLD+ SIIHALCFSPNRYWLCAATESSIKIWDLESKSIVVDL+VD
Sbjct: 148 GVILLWDLAEGKKLYSLDSGSIIHALCFSPNRYWLCAATESSIKIWDLESKSIVVDLRVD 207

Query: 473 LKTEADMITEGTTAQTNAGKNKVIYCTSLSWSADGSTLFSGYTDGVVRVWGIGR 312
           LK E +M    T+   NA K KVIYCTSLSWSADGSTLFSGYTDG++RVWGIGR
Sbjct: 208 LKQETEMFGNATSTSGNA-KTKVIYCTSLSWSADGSTLFSGYTDGLIRVWGIGR 260


>ref|NP_001233881.1| ArcA2 protein [Solanum lycopersicum] gi|4589836|dbj|BAA76896.1|
           LeArcA2 protein [Solanum lycopersicum]
          Length = 326

 Score =  308 bits (790), Expect = 7e-82
 Identities = 150/174 (86%), Positives = 158/174 (90%)
 Frame = -1

Query: 833 VRFSPNNVQPTIVSASWDKTVKIWNLNNCKLRSTLAGHNGYVNTVAVSPDGSLCASGGRD 654
           VRFSPN +QPTIVS SWD+TVKIWNL NCKLRSTLAGH+GYVNTVAVSPDGSLCASGG+D
Sbjct: 156 VRFSPNTLQPTIVSGSWDRTVKIWNLTNCKLRSTLAGHSGYVNTVAVSPDGSLCASGGKD 215

Query: 653 GVILLWDLAEGKKLYSLDATSIIHALCFSPNRYWLCAATESSIKIWDLESKSIVVDLKVD 474
           GVILLWDLAEGKKLYSLDA SIIH LCFSPNRYWLCAATESSIKIWDLESKSIVVDLKVD
Sbjct: 216 GVILLWDLAEGKKLYSLDAGSIIHTLCFSPNRYWLCAATESSIKIWDLESKSIVVDLKVD 275

Query: 473 LKTEADMITEGTTAQTNAGKNKVIYCTSLSWSADGSTLFSGYTDGVVRVWGIGR 312
           LK E++M  E  TA     K KVIYCTSLSWSADGSTLFSGYTDG++RVWGIGR
Sbjct: 276 LKHESEMANESGTAP----KTKVIYCTSLSWSADGSTLFSGYTDGLIRVWGIGR 325



 Score = 61.6 bits (148), Expect = 2e-07
 Identities = 56/204 (27%), Positives = 88/204 (43%), Gaps = 31/204 (15%)
 Frame = -1

Query: 824 SPNNVQPTIVSASWDKTVKIWNLNNCKL-----RSTLAGHNGYVNTVAVSPDGSLCASGG 660
           +P +    IV++S DK++ +W+L          R  L GH  +V  V +S DG    SG 
Sbjct: 24  TPIDNSDMIVTSSRDKSIIVWSLTKDGAQYGVPRRRLTGHGHFVQDVVLSSDGMFALSGS 83

Query: 659 RDGVILLWDLAEGKKLYS-LDATSIIHALCFS-PNRYWLCAATESSIKIWDL--ESKSIV 492
            DG + LWDL  G      +  T  + ++ FS  NR  + A+ + SIK+W+   E K  +
Sbjct: 84  WDGELRLWDLQAGTTARRFVGHTKDVLSVAFSVDNRQIVSASRDKSIKLWNTLGECKYTI 143

Query: 491 VD----------LKVDLKTEADMITEGTTAQ-------TNAGKNKVI-----YCTSLSWS 378
            D          ++    T    I  G+  +       TN      +     Y  +++ S
Sbjct: 144 QDGHSHSDWVSCVRFSPNTLQPTIVSGSWDRTVKIWNLTNCKLRSTLAGHSGYVNTVAVS 203

Query: 377 ADGSTLFSGYTDGVVRVWGIGR*K 306
            DGS   SG  DGV+ +W +   K
Sbjct: 204 PDGSLCASGGKDGVILLWDLAEGK 227


>ref|NP_001241455.1| uncharacterized protein LOC100778205 [Glycine max]
           gi|255645048|gb|ACU23023.1| unknown [Glycine max]
          Length = 325

 Score =  308 bits (789), Expect = 1e-81
 Identities = 149/174 (85%), Positives = 158/174 (90%)
 Frame = -1

Query: 833 VRFSPNNVQPTIVSASWDKTVKIWNLNNCKLRSTLAGHNGYVNTVAVSPDGSLCASGGRD 654
           VRFSP+ +QPTIVSASWD+TVK+WNL NCKLR+TLAGHNGYVNTVAVSPDGSLCASGG+D
Sbjct: 155 VRFSPSTLQPTIVSASWDRTVKVWNLTNCKLRNTLAGHNGYVNTVAVSPDGSLCASGGKD 214

Query: 653 GVILLWDLAEGKKLYSLDATSIIHALCFSPNRYWLCAATESSIKIWDLESKSIVVDLKVD 474
           GVILLWDLAEGK+LYSLDA SIIHALCFSPNRYWLCAATE SIKIWDLESKSIV DLKVD
Sbjct: 215 GVILLWDLAEGKRLYSLDAGSIIHALCFSPNRYWLCAATEQSIKIWDLESKSIVEDLKVD 274

Query: 473 LKTEADMITEGTTAQTNAGKNKVIYCTSLSWSADGSTLFSGYTDGVVRVWGIGR 312
           LKTEAD     TT   N  K KVIYCTSL+WS+DGSTLFSGYTDGVVRVWGIGR
Sbjct: 275 LKTEAD----ATTGGGNPNKKKVIYCTSLNWSSDGSTLFSGYTDGVVRVWGIGR 324



 Score = 65.1 bits (157), Expect = 2e-08
 Identities = 58/206 (28%), Positives = 88/206 (42%), Gaps = 33/206 (16%)
 Frame = -1

Query: 824 SPNNVQPTIVSASWDKTVKIWNLNNCKL-----RSTLAGHNGYVNTVAVSPDGSLCASGG 660
           +P +    IV+AS DK++ +W+L          R  L GH+ +V  V +S DG    SG 
Sbjct: 23  TPIDNSDMIVTASRDKSIILWHLTKEDKTYGVPRRRLTGHSHFVQDVVLSSDGQFALSGS 82

Query: 659 RDGVILLWDLAEGKKLYS-LDATSIIHALCFS-PNRYWLCAATESSIKIW---------- 516
            DG + LWDLA G      +  T  + ++ FS  NR  + A+ + +IK+W          
Sbjct: 83  WDGELRLWDLAAGTSARRFVGHTKDVLSVAFSIDNRQIVSASRDRTIKLWNTLGECKYTI 142

Query: 515 ---DLESKSI-------------VVDLKVDLKTEADMITEGTTAQTNAGKNKVIYCTSLS 384
              D  S  +             +V    D   +   +T      T AG N   Y  +++
Sbjct: 143 QDGDAHSDWVSCVRFSPSTLQPTIVSASWDRTVKVWNLTNCKLRNTLAGHNG--YVNTVA 200

Query: 383 WSADGSTLFSGYTDGVVRVWGIGR*K 306
            S DGS   SG  DGV+ +W +   K
Sbjct: 201 VSPDGSLCASGGKDGVILLWDLAEGK 226


>ref|XP_002281279.1| PREDICTED: guanine nucleotide-binding protein subunit beta-like
           protein isoform 1 [Vitis vinifera]
           gi|359491001|ref|XP_003634197.1| PREDICTED: guanine
           nucleotide-binding protein subunit beta-like protein
           isoform 2 [Vitis vinifera] gi|147784318|emb|CAN61810.1|
           hypothetical protein VITISV_026180 [Vitis vinifera]
          Length = 327

 Score =  308 bits (789), Expect = 1e-81
 Identities = 149/174 (85%), Positives = 159/174 (91%)
 Frame = -1

Query: 833 VRFSPNNVQPTIVSASWDKTVKIWNLNNCKLRSTLAGHNGYVNTVAVSPDGSLCASGGRD 654
           VRFSPNN+QPTIVSASWD+TVK+WNL NCKLR+TLAGH GYVNTVAVSPDGSLCASGG+D
Sbjct: 155 VRFSPNNLQPTIVSASWDRTVKVWNLTNCKLRATLAGHGGYVNTVAVSPDGSLCASGGKD 214

Query: 653 GVILLWDLAEGKKLYSLDATSIIHALCFSPNRYWLCAATESSIKIWDLESKSIVVDLKVD 474
           GVILLWDLAEGK+LYSLDA +IIHALCFSPNRYWLCAATE SIKIWDLESKSIV DLKVD
Sbjct: 215 GVILLWDLAEGKRLYSLDAGAIIHALCFSPNRYWLCAATEQSIKIWDLESKSIVEDLKVD 274

Query: 473 LKTEADMITEGTTAQTNAGKNKVIYCTSLSWSADGSTLFSGYTDGVVRVWGIGR 312
           LKTEA+   + TT  T   K K+IYCTSLSWSADGSTLFSGYTDGVVRVWGIGR
Sbjct: 275 LKTEAEKSEDTTT--TAKKKGKIIYCTSLSWSADGSTLFSGYTDGVVRVWGIGR 326



 Score = 62.4 bits (150), Expect = 1e-07
 Identities = 56/206 (27%), Positives = 88/206 (42%), Gaps = 33/206 (16%)
 Frame = -1

Query: 824 SPNNVQPTIVSASWDKTVKIWNLNNCKL-----RSTLAGHNGYVNTVAVSPDGSLCASGG 660
           +P +    IV++S DK++ +W+L          R  L GH+ +V  V +S DG    SG 
Sbjct: 23  TPIDNSDMIVTSSRDKSIILWHLTKDDKTYGVPRRRLTGHSHFVQDVVLSSDGQFALSGS 82

Query: 659 RDGVILLWDLAEGKKLYS-LDATSIIHALCFS-PNRYWLCAATESSIKIW---------- 516
            DG + LWDLA G      +  T  + ++ FS  NR  + A+ + +IK+W          
Sbjct: 83  WDGELRLWDLAAGTSARRFVGHTKDVLSVAFSVDNRQIVSASRDRTIKLWNTLGECKYTI 142

Query: 515 -DLESKS---------------IVVDLKVDLKTEADMITEGTTAQTNAGKNKVIYCTSLS 384
            D ++ S                +V    D   +   +T      T AG     Y  +++
Sbjct: 143 QDADAHSDWVSCVRFSPNNLQPTIVSASWDRTVKVWNLTNCKLRATLAGHGG--YVNTVA 200

Query: 383 WSADGSTLFSGYTDGVVRVWGIGR*K 306
            S DGS   SG  DGV+ +W +   K
Sbjct: 201 VSPDGSLCASGGKDGVILLWDLAEGK 226