BLASTX nr result
ID: Bupleurum21_contig00003036
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00003036
(2167 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002274214.2| PREDICTED: L-galactono-1,4-lactone dehydroge... 768 0.0
emb|CBI30081.3| unnamed protein product [Vitis vinifera] 765 0.0
gb|ACN59217.1| putative L-galactono-1,4-lactone dehydrogenase [V... 764 0.0
gb|ACO92659.1| mitochondrial L-galactono-1,4-lactone dehydrogena... 755 0.0
gb|ACG75919.1| L-galactono-1,4-lactone dehydrogenase [Malpighia ... 753 0.0
>ref|XP_002274214.2| PREDICTED: L-galactono-1,4-lactone dehydrogenase, mitochondrial
[Vitis vinifera]
Length = 609
Score = 768 bits (1982), Expect = 0.0
Identities = 369/439 (84%), Positives = 410/439 (93%)
Frame = -3
Query: 1547 GMVNLALLDKILEVDKEKKIVKVQAGIRVQQLVDGIKDYGITLQNFASIREQQIGGIIQV 1368
GMVNLAL+D +L+VD EKK V+VQAGIRVQQLVD IKDYGITLQNFASIREQQIGGI+QV
Sbjct: 166 GMVNLALMDNVLDVDVEKKRVRVQAGIRVQQLVDAIKDYGITLQNFASIREQQIGGIVQV 225
Query: 1367 GAHGTGARLPPIDEQVISMKLVTPAKGTIEVSRDKDPELFYLARCGLGGLGVVSEVTLQC 1188
GAHGTGARLPPIDEQVISMKLVTPAKGTIEVS+++DPELFYLARCGLGGLGVV+EVTLQC
Sbjct: 226 GAHGTGARLPPIDEQVISMKLVTPAKGTIEVSKERDPELFYLARCGLGGLGVVAEVTLQC 285
Query: 1187 VERQELVEHTFVSNIKDIKKNHKKLLSDNKHVKYLYIPYTDTVVVVRCNPVSKWKGIPNF 1008
VERQELVEHT VSN+K+IKKNHKKLLS+NKHVKYLYIPYTDTVVVV CNPVSKWKG P F
Sbjct: 286 VERQELVEHTVVSNMKEIKKNHKKLLSENKHVKYLYIPYTDTVVVVTCNPVSKWKGPPKF 345
Query: 1007 KPKYSKDEAMKQIRDLYYESMKKYRGESPDSKTPGETGPEISELTFTELRDKLLALDPLN 828
KPKYS+DEA++ +RDLY ES+KKY E+ +K+ + P+I+EL+FTELRDKLLALDPLN
Sbjct: 346 KPKYSRDEAIQHVRDLYQESLKKYSPEAITAKSSDNSEPDINELSFTELRDKLLALDPLN 405
Query: 827 KDHVIKVNQAEAEFWRRSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEY 648
KDHVI+VNQAEAEFWR+SEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKP+MKD+EY
Sbjct: 406 KDHVIQVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPSMKDLEY 465
Query: 647 IEQVLQLIEKEQVPAPAPIEQRWTTSSKSPMSPAYSLSEDDVFSWVGIIMYLPTNDSRQR 468
IE + +LIEKE++PAPAPIEQRWT SSKSPMSPA S +EDD+FSWVGIIMYLPT D+RQR
Sbjct: 466 IEDLKKLIEKEEIPAPAPIEQRWTQSSKSPMSPASSSAEDDIFSWVGIIMYLPTMDARQR 525
Query: 467 KEITEEFFHYRHMTQTQLWDQYSAYEHWAKIEVPKDKDELAALQSRLRKRFPVEAYNKAR 288
KEITEEFFHYR ++QTQLWD YSAYEHWAKIEVPKDKDELAALQ+RLRKRFPV+ YNKAR
Sbjct: 526 KEITEEFFHYRRLSQTQLWDLYSAYEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKAR 585
Query: 287 RELDPNSVLSNNMLMKLFP 231
+ELDPN +LSNNML KLFP
Sbjct: 586 KELDPNRILSNNMLGKLFP 604
Score = 144 bits (362), Expect = 1e-31
Identities = 78/142 (54%), Positives = 93/142 (65%), Gaps = 9/142 (6%)
Frame = -1
Query: 2089 MSRALNLKRSLKTLLHHHNAHFS---------KTLISTTTQSPLLNPNTNHLFLRRFXXX 1937
M RAL+L+RSL+ H+H+ H K L STT+ +P NPN F
Sbjct: 1 MLRALSLRRSLRAFHHYHHHHHQPTNPHSNLLKALSSTTSPNP--NPNLTRPF------- 51
Query: 1936 XXXXXXXXXXSDAELRKYMGYGALLIGCAAATYYSFPFPENAKHKKAQIFRYAPLPEDLH 1757
S+AE RKY+GY ALL+GC ATYYSFPF ++AKHKKAQ+FRYAPLP+DLH
Sbjct: 52 --SSLSSSPSSEAEFRKYLGYFALLLGCGIATYYSFPFSDSAKHKKAQLFRYAPLPDDLH 109
Query: 1756 TVSNWSGTHEVQTRNFFQPESV 1691
TVSNWSGTHEVQTR F QPES+
Sbjct: 110 TVSNWSGTHEVQTRVFHQPESL 131
>emb|CBI30081.3| unnamed protein product [Vitis vinifera]
Length = 443
Score = 765 bits (1976), Expect = 0.0
Identities = 368/438 (84%), Positives = 409/438 (93%)
Frame = -3
Query: 1544 MVNLALLDKILEVDKEKKIVKVQAGIRVQQLVDGIKDYGITLQNFASIREQQIGGIIQVG 1365
MVNLAL+D +L+VD EKK V+VQAGIRVQQLVD IKDYGITLQNFASIREQQIGGI+QVG
Sbjct: 1 MVNLALMDNVLDVDVEKKRVRVQAGIRVQQLVDAIKDYGITLQNFASIREQQIGGIVQVG 60
Query: 1364 AHGTGARLPPIDEQVISMKLVTPAKGTIEVSRDKDPELFYLARCGLGGLGVVSEVTLQCV 1185
AHGTGARLPPIDEQVISMKLVTPAKGTIEVS+++DPELFYLARCGLGGLGVV+EVTLQCV
Sbjct: 61 AHGTGARLPPIDEQVISMKLVTPAKGTIEVSKERDPELFYLARCGLGGLGVVAEVTLQCV 120
Query: 1184 ERQELVEHTFVSNIKDIKKNHKKLLSDNKHVKYLYIPYTDTVVVVRCNPVSKWKGIPNFK 1005
ERQELVEHT VSN+K+IKKNHKKLLS+NKHVKYLYIPYTDTVVVV CNPVSKWKG P FK
Sbjct: 121 ERQELVEHTVVSNMKEIKKNHKKLLSENKHVKYLYIPYTDTVVVVTCNPVSKWKGPPKFK 180
Query: 1004 PKYSKDEAMKQIRDLYYESMKKYRGESPDSKTPGETGPEISELTFTELRDKLLALDPLNK 825
PKYS+DEA++ +RDLY ES+KKY E+ +K+ + P+I+EL+FTELRDKLLALDPLNK
Sbjct: 181 PKYSRDEAIQHVRDLYQESLKKYSPEAITAKSSDNSEPDINELSFTELRDKLLALDPLNK 240
Query: 824 DHVIKVNQAEAEFWRRSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYI 645
DHVI+VNQAEAEFWR+SEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKP+MKD+EYI
Sbjct: 241 DHVIQVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPSMKDLEYI 300
Query: 644 EQVLQLIEKEQVPAPAPIEQRWTTSSKSPMSPAYSLSEDDVFSWVGIIMYLPTNDSRQRK 465
E + +LIEKE++PAPAPIEQRWT SSKSPMSPA S +EDD+FSWVGIIMYLPT D+RQRK
Sbjct: 301 EDLKKLIEKEEIPAPAPIEQRWTQSSKSPMSPASSSAEDDIFSWVGIIMYLPTMDARQRK 360
Query: 464 EITEEFFHYRHMTQTQLWDQYSAYEHWAKIEVPKDKDELAALQSRLRKRFPVEAYNKARR 285
EITEEFFHYR ++QTQLWD YSAYEHWAKIEVPKDKDELAALQ+RLRKRFPV+ YNKAR+
Sbjct: 361 EITEEFFHYRRLSQTQLWDLYSAYEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKARK 420
Query: 284 ELDPNSVLSNNMLMKLFP 231
ELDPN +LSNNML KLFP
Sbjct: 421 ELDPNRILSNNMLGKLFP 438
>gb|ACN59217.1| putative L-galactono-1,4-lactone dehydrogenase [Vitis vinifera]
Length = 611
Score = 764 bits (1972), Expect = 0.0
Identities = 369/439 (84%), Positives = 407/439 (92%)
Frame = -3
Query: 1547 GMVNLALLDKILEVDKEKKIVKVQAGIRVQQLVDGIKDYGITLQNFASIREQQIGGIIQV 1368
GMVNLALLD +L+VD EKK V+VQAGIRVQQLVD IKDYGITLQNFASIREQQIGGI+QV
Sbjct: 168 GMVNLALLDNVLDVDVEKKRVRVQAGIRVQQLVDAIKDYGITLQNFASIREQQIGGIVQV 227
Query: 1367 GAHGTGARLPPIDEQVISMKLVTPAKGTIEVSRDKDPELFYLARCGLGGLGVVSEVTLQC 1188
GAHGTGARLPPIDEQVISMKLVTPAKGTIEVS++KDPELFYLARCGLGGLGVV+EVTLQC
Sbjct: 228 GAHGTGARLPPIDEQVISMKLVTPAKGTIEVSKEKDPELFYLARCGLGGLGVVAEVTLQC 287
Query: 1187 VERQELVEHTFVSNIKDIKKNHKKLLSDNKHVKYLYIPYTDTVVVVRCNPVSKWKGIPNF 1008
VERQELVEHT VSN+K+IKKNHKKLLS+NKHVKYLYIPYTDTVVVV CNPVSKWKG P F
Sbjct: 288 VERQELVEHTVVSNMKEIKKNHKKLLSENKHVKYLYIPYTDTVVVVTCNPVSKWKGPPKF 347
Query: 1007 KPKYSKDEAMKQIRDLYYESMKKYRGESPDSKTPGETGPEISELTFTELRDKLLALDPLN 828
KPKYS DEA++ +R LY ES+KKY E+ +K+ + P+I+EL+FTELRDKLLALDPLN
Sbjct: 348 KPKYSHDEAIQHVRHLYQESLKKYSPEAITAKSSDNSEPDINELSFTELRDKLLALDPLN 407
Query: 827 KDHVIKVNQAEAEFWRRSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEY 648
KDHVI+VNQAEAEFWR+SEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKP+MKD+EY
Sbjct: 408 KDHVIQVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPSMKDLEY 467
Query: 647 IEQVLQLIEKEQVPAPAPIEQRWTTSSKSPMSPAYSLSEDDVFSWVGIIMYLPTNDSRQR 468
IE + +LIEKE++PAPAPIEQRWT SSKSPMSPA S +EDD+FSWVGIIMYLPT D+RQR
Sbjct: 468 IEDLKKLIEKEEIPAPAPIEQRWTASSKSPMSPASSSAEDDIFSWVGIIMYLPTMDARQR 527
Query: 467 KEITEEFFHYRHMTQTQLWDQYSAYEHWAKIEVPKDKDELAALQSRLRKRFPVEAYNKAR 288
KEITEEFFHYR ++QTQLWD YSAYEHWAKIEVPKDKDELAALQ+RLRKRFPV+ YNKAR
Sbjct: 528 KEITEEFFHYRRLSQTQLWDLYSAYEHWAKIEVPKDKDELAALQARLRKRFPVDEYNKAR 587
Query: 287 RELDPNSVLSNNMLMKLFP 231
+ELDPN +LSNNM KLFP
Sbjct: 588 KELDPNRILSNNMQGKLFP 606
Score = 142 bits (359), Expect = 3e-31
Identities = 78/144 (54%), Positives = 93/144 (64%), Gaps = 11/144 (7%)
Frame = -1
Query: 2089 MSRALNLKRSLKTLLHHHNAHFS-----------KTLISTTTQSPLLNPNTNHLFLRRFX 1943
M RAL+L+RSL+ H+H+ H K L STT+ +P NPN F
Sbjct: 1 MLRALSLRRSLQAFHHYHHHHHQQQPTNPHSNLLKALSSTTSPNP--NPNLTRPF----- 53
Query: 1942 XXXXXXXXXXXXSDAELRKYMGYGALLIGCAAATYYSFPFPENAKHKKAQIFRYAPLPED 1763
S+AE RKY+GY ALL+GC ATYYSFPF ++AKHKKAQ+FRYAPLP+D
Sbjct: 54 ----SSLSSSPSSEAEFRKYLGYFALLLGCGIATYYSFPFSDSAKHKKAQLFRYAPLPDD 109
Query: 1762 LHTVSNWSGTHEVQTRNFFQPESV 1691
LHTVSNWSGTHEVQTR F QPES+
Sbjct: 110 LHTVSNWSGTHEVQTRVFHQPESL 133
>gb|ACO92659.1| mitochondrial L-galactono-1,4-lactone dehydrogenase [Citrus unshiu]
Length = 605
Score = 755 bits (1950), Expect = 0.0
Identities = 362/440 (82%), Positives = 409/440 (92%)
Frame = -3
Query: 1547 GMVNLALLDKILEVDKEKKIVKVQAGIRVQQLVDGIKDYGITLQNFASIREQQIGGIIQV 1368
GMVNLALLDK+LEVDKEKK V+VQAGIRVQ LVD IK YG+TLQNFASIREQQIGGI+QV
Sbjct: 162 GMVNLALLDKVLEVDKEKKRVRVQAGIRVQGLVDEIKQYGLTLQNFASIREQQIGGIVQV 221
Query: 1367 GAHGTGARLPPIDEQVISMKLVTPAKGTIEVSRDKDPELFYLARCGLGGLGVVSEVTLQC 1188
GAHGTGA+LPP+DEQVISMKLVTPAKGTIEVS++KDP+LFYLARCGLGGLGVV+EVTLQC
Sbjct: 222 GAHGTGAKLPPVDEQVISMKLVTPAKGTIEVSKEKDPDLFYLARCGLGGLGVVAEVTLQC 281
Query: 1187 VERQELVEHTFVSNIKDIKKNHKKLLSDNKHVKYLYIPYTDTVVVVRCNPVSKWKGIPNF 1008
VERQELVEHT VSNIK+IKKNHKKLLS+NKHVKYL+IPYTDTVVVV CNPVSKWKG P F
Sbjct: 282 VERQELVEHTTVSNIKEIKKNHKKLLSENKHVKYLHIPYTDTVVVVTCNPVSKWKGPPKF 341
Query: 1007 KPKYSKDEAMKQIRDLYYESMKKYRGESPDSKTPGETGPEISELTFTELRDKLLALDPLN 828
KPKY+K+EA++ + DLY ES+KKYR + +K+P T P+I+EL+FTELRDKLLALDPLN
Sbjct: 342 KPKYTKNEALQHLPDLYRESLKKYRADVMTAKSPDGTEPDINELSFTELRDKLLALDPLN 401
Query: 827 KDHVIKVNQAEAEFWRRSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEY 648
K+HVIKVNQAEAEFWR+SEGYRVGW+DEILGFDCGGQQWVSETCFP+GTLAK +MKD+EY
Sbjct: 402 KEHVIKVNQAEAEFWRKSEGYRVGWADEILGFDCGGQQWVSETCFPSGTLAKLSMKDLEY 461
Query: 647 IEQVLQLIEKEQVPAPAPIEQRWTTSSKSPMSPAYSLSEDDVFSWVGIIMYLPTNDSRQR 468
E++ QLIEKE +PAPAPIEQRWT S+S MSPAYS +DD+FSWVGIIMYLPT D+RQR
Sbjct: 462 TEELKQLIEKEDIPAPAPIEQRWTARSQSVMSPAYSSVQDDIFSWVGIIMYLPTMDARQR 521
Query: 467 KEITEEFFHYRHMTQTQLWDQYSAYEHWAKIEVPKDKDELAALQSRLRKRFPVEAYNKAR 288
KEIT+EFF+YRH+TQ QLWDQYSAYEHWAKIEVPKDK+ELAALQ+RLRKRFPV++YNKAR
Sbjct: 522 KEITDEFFNYRHLTQKQLWDQYSAYEHWAKIEVPKDKEELAALQARLRKRFPVDSYNKAR 581
Query: 287 RELDPNSVLSNNMLMKLFPL 228
+ELDPN +LSNNML KLFPL
Sbjct: 582 KELDPNRILSNNMLEKLFPL 601
Score = 146 bits (368), Expect = 3e-32
Identities = 80/144 (55%), Positives = 95/144 (65%), Gaps = 11/144 (7%)
Frame = -1
Query: 2089 MSRALNLKRSLKTLLH-----------HHNAHFSKTLISTTTQSPLLNPNTNHLFLRRFX 1943
M RAL+++R+L++L H HH +TL ST + SP +P+ +
Sbjct: 1 MQRALSIRRTLQSLHHRLLFNKSVPLKHHPLPSIRTLSSTPSASPSPSPSPS-------- 52
Query: 1942 XXXXXXXXXXXXSDAELRKYMGYGALLIGCAAATYYSFPFPENAKHKKAQIFRYAPLPED 1763
SDAE+RKY+GY ALL+ C AATYYSFPF E+AKHKKAQIFRYAPLPED
Sbjct: 53 ---------PSPSDAEIRKYLGYTALLVFCGAATYYSFPFSEDAKHKKAQIFRYAPLPED 103
Query: 1762 LHTVSNWSGTHEVQTRNFFQPESV 1691
LHTVSNWSGTHEVQTRNF QPESV
Sbjct: 104 LHTVSNWSGTHEVQTRNFHQPESV 127
>gb|ACG75919.1| L-galactono-1,4-lactone dehydrogenase [Malpighia glabra]
Length = 592
Score = 753 bits (1944), Expect = 0.0
Identities = 356/439 (81%), Positives = 403/439 (91%)
Frame = -3
Query: 1547 GMVNLALLDKILEVDKEKKIVKVQAGIRVQQLVDGIKDYGITLQNFASIREQQIGGIIQV 1368
GMVNLAL+DK+LEVDKEKK V+VQAGIRVQ+LVDGIK++G+TLQNFASIREQQIGGI+QV
Sbjct: 149 GMVNLALVDKVLEVDKEKKRVRVQAGIRVQELVDGIKEHGLTLQNFASIREQQIGGIVQV 208
Query: 1367 GAHGTGARLPPIDEQVISMKLVTPAKGTIEVSRDKDPELFYLARCGLGGLGVVSEVTLQC 1188
GAHGTGARLPPIDEQVISMKLVTPAKGTIE+S+DKDPELFYLARCGLGGLGVV+EVTLQC
Sbjct: 209 GAHGTGARLPPIDEQVISMKLVTPAKGTIEISKDKDPELFYLARCGLGGLGVVAEVTLQC 268
Query: 1187 VERQELVEHTFVSNIKDIKKNHKKLLSDNKHVKYLYIPYTDTVVVVRCNPVSKWKGIPNF 1008
VERQ+LVEHT++SN+KDI+KNHKKLLSDNKHVKYLYIPYTD VVVV CNPVSKW+G+P F
Sbjct: 269 VERQQLVEHTYISNMKDIRKNHKKLLSDNKHVKYLYIPYTDAVVVVTCNPVSKWRGVPKF 328
Query: 1007 KPKYSKDEAMKQIRDLYYESMKKYRGESPDSKTPGETGPEISELTFTELRDKLLALDPLN 828
PKY++DEA++ +RDLY E + KYRG SK+ + P+I+EL+FTELRDKLLALDPLN
Sbjct: 329 TPKYTEDEALQHVRDLYQEPLNKYRGGEITSKSSEDDSPDINELSFTELRDKLLALDPLN 388
Query: 827 KDHVIKVNQAEAEFWRRSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEY 648
KDHVIKVNQAEAEFWR+SEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLA P+M+D++Y
Sbjct: 389 KDHVIKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLANPSMQDLDY 448
Query: 647 IEQVLQLIEKEQVPAPAPIEQRWTTSSKSPMSPAYSLSEDDVFSWVGIIMYLPTNDSRQR 468
IE + QLIEKE +PAPAPIEQRWT S+S MS A S EDD+FSWVGIIMYLPT D+RQR
Sbjct: 449 IEDLKQLIEKEDIPAPAPIEQRWTARSQSSMSVASSSKEDDIFSWVGIIMYLPTMDARQR 508
Query: 467 KEITEEFFHYRHMTQTQLWDQYSAYEHWAKIEVPKDKDELAALQSRLRKRFPVEAYNKAR 288
KEITEEFFHYRH+TQ +LWD+YSAYEHWAKIEVPKDK+EL AL RL+KRFPV+AYNKAR
Sbjct: 509 KEITEEFFHYRHLTQAELWDKYSAYEHWAKIEVPKDKEELEALLERLKKRFPVDAYNKAR 568
Query: 287 RELDPNSVLSNNMLMKLFP 231
+ELDPN +LSNN L KLFP
Sbjct: 569 KELDPNKILSNNKLEKLFP 587
Score = 132 bits (333), Expect = 3e-28
Identities = 60/71 (84%), Positives = 65/71 (91%)
Frame = -1
Query: 1903 DAELRKYMGYGALLIGCAAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV 1724
D+E+RKY+GY AL I C AATYYSFPF ENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV
Sbjct: 44 DSEVRKYLGYTALFIFCGAATYYSFPFSENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV 103
Query: 1723 QTRNFFQPESV 1691
QTRNF QPE++
Sbjct: 104 QTRNFHQPETI 114