BLASTX nr result
ID: Bupleurum21_contig00003011
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00003011
(2147 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AEO72339.1| asparagine synthetase 1 [Vitis vinifera] 570 0.0
ref|XP_002277103.1| PREDICTED: asparagine synthetase [glutamine-... 570 0.0
ref|XP_002526709.1| asparagine synthetase, putative [Ricinus com... 562 0.0
gb|AAF74755.1| asparagine synthetase [Helianthus annuus] 570 0.0
ref|XP_002299991.1| predicted protein [Populus trichocarpa] gi|2... 551 0.0
>gb|AEO72339.1| asparagine synthetase 1 [Vitis vinifera]
Length = 583
Score = 570 bits (1468), Expect(2) = 0.0
Identities = 286/373 (76%), Positives = 305/373 (81%), Gaps = 7/373 (1%)
Frame = -3
Query: 1497 RQAFENAVIKRLMTDVPFXXXXXXXXXXXXXXSITARHLAGTKAAKQWGAQLHSFCVGLE 1318
R+AFENAVIKRLMTDVPF SITARHLAGTKAAKQWGAQLHSFCVGLE
Sbjct: 211 RRAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKQWGAQLHSFCVGLE 270
Query: 1317 GSPDLKAGKEVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRK 1138
GSPDLKA KEVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRK
Sbjct: 271 GSPDLKAAKEVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRK 330
Query: 1137 IKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHRETCHKIKALHQYDCLRANKSTSAW 958
IKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHRETC KIKAL+QYDCLRANKSTSAW
Sbjct: 331 IKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHRETCRKIKALYQYDCLRANKSTSAW 390
Query: 957 GLEARVPFLDKEFINVAMSIDPEWKMIKPEEKRIEKWILRKAFDDEERPYLPKHILYRQK 778
GLEARVPFLDKEFI VAM IDPEWKMIKPE+ RIEKW+LR+AFDDEE+PYLPKHILYRQK
Sbjct: 391 GLEARVPFLDKEFIKVAMDIDPEWKMIKPEQGRIEKWVLRRAFDDEEQPYLPKHILYRQK 450
Query: 777 EQFSDGVGYSWI-------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXERFFPQNS 619
EQFSDGVGYSWI ERFFPQNS
Sbjct: 451 EQFSDGVGYSWIDGLKAHASQHVTDKMMLNASHIFPHNTPTTKEAYYYRMIFERFFPQNS 510
Query: 618 ARLTVPGGPSIACSTAKAVEWDASWSNNLDPSGRSALGVHNSAYEKQVASTVNKNLGTQI 439
ARLTVPGG S+ACSTAKAVEWD++WSNNLDPSGR+ALGVH SAY++++ + N+ T+I
Sbjct: 511 ARLTVPGGASVACSTAKAVEWDSAWSNNLDPSGRAALGVHLSAYDQKLTTVSAANVPTKI 570
Query: 438 IDNVPRMMEVGAP 400
IDN+PR+MEV AP
Sbjct: 571 IDNMPRIMEVTAP 583
Score = 363 bits (932), Expect(2) = 0.0
Identities = 170/181 (93%), Positives = 179/181 (98%)
Frame = -1
Query: 2138 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQYGDSYLAHQRLAIIDPASGD 1959
MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQ+GD YLAHQRLAIIDPASGD
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHGDCYLAHQRLAIIDPASGD 60
Query: 1958 QPLYNEDETIVVTVNGEIYNHEELRKSLPNHKFKTGSDCDVIAHLYEEHGENFVDMLDGM 1779
QPLYNE++ IVVTVNGEIYNHEELRKSLPNHKF+TGSDCDVIAHLYEEHGENFVDMLDGM
Sbjct: 61 QPLYNENQAIVVTVNGEIYNHEELRKSLPNHKFRTGSDCDVIAHLYEEHGENFVDMLDGM 120
Query: 1778 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSIWIASELKGLNDDCEHFEAFPPGHLY 1599
FSFVLLDTRD+SFIVARDAIGITSLYIGWGLDGS+WI+SELKGLNDDCEHFE+FPPGH+Y
Sbjct: 121 FSFVLLDTRDDSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFESFPPGHMY 180
Query: 1598 S 1596
S
Sbjct: 181 S 181
>ref|XP_002277103.1| PREDICTED: asparagine synthetase [glutamine-hydrolyzing] [Vitis
vinifera] gi|297745876|emb|CBI15932.3| unnamed protein
product [Vitis vinifera]
Length = 583
Score = 570 bits (1468), Expect(2) = 0.0
Identities = 286/373 (76%), Positives = 305/373 (81%), Gaps = 7/373 (1%)
Frame = -3
Query: 1497 RQAFENAVIKRLMTDVPFXXXXXXXXXXXXXXSITARHLAGTKAAKQWGAQLHSFCVGLE 1318
R+AFENAVIKRLMTDVPF SITARHLAGTKAAKQWGAQLHSFCVGLE
Sbjct: 211 RRAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLAGTKAAKQWGAQLHSFCVGLE 270
Query: 1317 GSPDLKAGKEVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRK 1138
GSPDLKA KEVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRK
Sbjct: 271 GSPDLKAAKEVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRK 330
Query: 1137 IKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHRETCHKIKALHQYDCLRANKSTSAW 958
IKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHRETC KIKAL+QYDCLRANKSTSAW
Sbjct: 331 IKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHRETCRKIKALYQYDCLRANKSTSAW 390
Query: 957 GLEARVPFLDKEFINVAMSIDPEWKMIKPEEKRIEKWILRKAFDDEERPYLPKHILYRQK 778
GLEARVPFLDKEFI VAM IDPEWKMIKPE+ RIEKW+LR+AFDDEE+PYLPKHILYRQK
Sbjct: 391 GLEARVPFLDKEFIKVAMDIDPEWKMIKPEQGRIEKWVLRRAFDDEEQPYLPKHILYRQK 450
Query: 777 EQFSDGVGYSWI-------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXERFFPQNS 619
EQFSDGVGYSWI ERFFPQNS
Sbjct: 451 EQFSDGVGYSWIDGLKAHASQHVTDKMMLNASHIFPHNTPTTKEAYYYRMIFERFFPQNS 510
Query: 618 ARLTVPGGPSIACSTAKAVEWDASWSNNLDPSGRSALGVHNSAYEKQVASTVNKNLGTQI 439
ARLTVPGG S+ACSTAKAVEWD++WSNNLDPSGR+ALGVH SAY++++ + N+ T+I
Sbjct: 511 ARLTVPGGASVACSTAKAVEWDSAWSNNLDPSGRAALGVHLSAYDQKLTTVSAANVPTKI 570
Query: 438 IDNVPRMMEVGAP 400
IDN+PR+MEV AP
Sbjct: 571 IDNMPRIMEVTAP 583
Score = 362 bits (930), Expect(2) = 0.0
Identities = 169/181 (93%), Positives = 179/181 (98%)
Frame = -1
Query: 2138 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQYGDSYLAHQRLAIIDPASGD 1959
MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQ+GD YLAHQRLAIIDPASGD
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHGDCYLAHQRLAIIDPASGD 60
Query: 1958 QPLYNEDETIVVTVNGEIYNHEELRKSLPNHKFKTGSDCDVIAHLYEEHGENFVDMLDGM 1779
QPLYNE++ IVVTVNGEIYNHEELRKS+PNHKF+TGSDCDVIAHLYEEHGENFVDMLDGM
Sbjct: 61 QPLYNENQAIVVTVNGEIYNHEELRKSMPNHKFRTGSDCDVIAHLYEEHGENFVDMLDGM 120
Query: 1778 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSIWIASELKGLNDDCEHFEAFPPGHLY 1599
FSFVLLDTRD+SFIVARDAIGITSLYIGWGLDGS+WI+SELKGLNDDCEHFE+FPPGH+Y
Sbjct: 121 FSFVLLDTRDDSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFESFPPGHMY 180
Query: 1598 S 1596
S
Sbjct: 181 S 181
>ref|XP_002526709.1| asparagine synthetase, putative [Ricinus communis]
gi|223533948|gb|EEF35671.1| asparagine synthetase,
putative [Ricinus communis]
Length = 591
Score = 562 bits (1449), Expect(2) = 0.0
Identities = 289/381 (75%), Positives = 305/381 (80%), Gaps = 9/381 (2%)
Frame = -3
Query: 1497 RQAFENAVIKRLMTDVPFXXXXXXXXXXXXXXSITARHLAGTKAAKQWGAQLHSFCVGLE 1318
R+AFENAVIKRLMTDVPF SITARHLA TKAAKQWGAQLHSFCVGLE
Sbjct: 211 RRAFENAVIKRLMTDVPFGVLLSGGLDSSLVASITARHLADTKAAKQWGAQLHSFCVGLE 270
Query: 1317 GSPDLKAGKEVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRK 1138
GSPDLKA KEVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLM+RK
Sbjct: 271 GSPDLKAAKEVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMARK 330
Query: 1137 IKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHRETCHKIKALHQYDCLRANKSTSAW 958
IK+LGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHRETCHKIKALHQYDCLRANK+TSAW
Sbjct: 331 IKALGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHRETCHKIKALHQYDCLRANKATSAW 390
Query: 957 GLEARVPFLDKEFINVAMSIDPEWKMIKPEEKRIEKWILRKAFDDEERPYLPKHILYRQK 778
GLEAR+PFLDKEFINVAM+I PE KMIKPEE RIEKW+LR+AFDDEE PYLPKHILYRQK
Sbjct: 391 GLEARIPFLDKEFINVAMAIGPESKMIKPEEGRIEKWVLRRAFDDEEHPYLPKHILYRQK 450
Query: 777 EQFSDGVGYSWI-------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXERFFPQNS 619
EQFSDGVGYSWI ERFFPQNS
Sbjct: 451 EQFSDGVGYSWIDGLKAHAAEHVTDKMIQNAEHIFTHNTPTTKEAYYYRMIFERFFPQNS 510
Query: 618 ARLTVPGGPSIACSTAKAVEWDASWSNNLDPSGRSALGVHNSAYEKQVASTVNK--NLGT 445
ARLTVPGG SIACSTAKAVEWDA+WS+NLDPSGR+ALGVH SAY KQ A+ V +
Sbjct: 511 ARLTVPGGASIACSTAKAVEWDAAWSDNLDPSGRAALGVHLSAYGKQAAAAVASAGMVPP 570
Query: 444 QIIDNVPRMMEVGAPELTIRS 382
+IID++PRMMEV P LTI S
Sbjct: 571 KIIDSMPRMMEVSPPGLTISS 591
Score = 358 bits (920), Expect(2) = 0.0
Identities = 170/181 (93%), Positives = 177/181 (97%)
Frame = -1
Query: 2138 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQYGDSYLAHQRLAIIDPASGD 1959
MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQ+GDSYLAHQRLAIIDPASGD
Sbjct: 1 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQHGDSYLAHQRLAIIDPASGD 60
Query: 1958 QPLYNEDETIVVTVNGEIYNHEELRKSLPNHKFKTGSDCDVIAHLYEEHGENFVDMLDGM 1779
QPLYNED+ IVVTVNGEIYNHEELRK L +HKF+TGSDCDVIAHLYEE+GE+FVDMLDGM
Sbjct: 61 QPLYNEDKAIVVTVNGEIYNHEELRKKLSHHKFRTGSDCDVIAHLYEEYGEDFVDMLDGM 120
Query: 1778 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSIWIASELKGLNDDCEHFEAFPPGHLY 1599
FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSIWI+SELKGLNDDCEHFE FPPGHLY
Sbjct: 121 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSIWISSELKGLNDDCEHFECFPPGHLY 180
Query: 1598 S 1596
S
Sbjct: 181 S 181
>gb|AAF74755.1| asparagine synthetase [Helianthus annuus]
Length = 589
Score = 570 bits (1469), Expect(2) = 0.0
Identities = 287/379 (75%), Positives = 305/379 (80%), Gaps = 7/379 (1%)
Frame = -3
Query: 1497 RQAFENAVIKRLMTDVPFXXXXXXXXXXXXXXSITARHLAGTKAAKQWGAQLHSFCVGLE 1318
R AFENAVIKRLMTDVPF S+TARHLAG+KAAKQWGAQLHSFCVGLE
Sbjct: 211 RCAFENAVIKRLMTDVPFGVLLSGGLDSSLVASVTARHLAGSKAAKQWGAQLHSFCVGLE 270
Query: 1317 GSPDLKAGKEVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRK 1138
GSPDLKA +EVADYLGT+HHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRK
Sbjct: 271 GSPDLKAAREVADYLGTLHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRK 330
Query: 1137 IKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHRETCHKIKALHQYDCLRANKSTSAW 958
IKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFH+ETC KIKALHQYDCLRANKSTSAW
Sbjct: 331 IKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHQETCRKIKALHQYDCLRANKSTSAW 390
Query: 957 GLEARVPFLDKEFINVAMSIDPEWKMIKPEEKRIEKWILRKAFDDEERPYLPKHILYRQK 778
GLEARVPFLDKEFINVAMSIDPE KMI ++KRIEKWILR+AFDDEE+PYLPKHILYRQK
Sbjct: 391 GLEARVPFLDKEFINVAMSIDPEAKMINMDQKRIEKWILRRAFDDEEKPYLPKHILYRQK 450
Query: 777 EQFSDGVGYSWI-------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXERFFPQNS 619
EQFSDGVGYSWI ERFFPQNS
Sbjct: 451 EQFSDGVGYSWIDGLKSHAEQHVTNKMMLNAERIFPHNTPVTKEAYYYRMIFERFFPQNS 510
Query: 618 ARLTVPGGPSIACSTAKAVEWDASWSNNLDPSGRSALGVHNSAYEKQVASTVNKNLGTQI 439
A+LTVPGG S+ACSTAKA+EWDASWSNNLDPSGR+ALGVHN+AY+K S + NL I
Sbjct: 511 AKLTVPGGASVACSTAKAIEWDASWSNNLDPSGRAALGVHNAAYKKNSGSISSANLAPSI 570
Query: 438 IDNVPRMMEVGAPELTIRS 382
DNVPRMM++ PEL IRS
Sbjct: 571 ADNVPRMMDITGPELVIRS 589
Score = 337 bits (863), Expect(2) = 0.0
Identities = 159/181 (87%), Positives = 170/181 (93%)
Frame = -1
Query: 2138 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQYGDSYLAHQRLAIIDPASGD 1959
MCGILA+LGCSDDSQAKR RVLELSRRLKHRGPDWSGLYQ+GD+YL+HQRLA+IDPASGD
Sbjct: 1 MCGILALLGCSDDSQAKRFRVLELSRRLKHRGPDWSGLYQHGDNYLSHQRLAVIDPASGD 60
Query: 1958 QPLYNEDETIVVTVNGEIYNHEELRKSLPNHKFKTGSDCDVIAHLYEEHGENFVDMLDGM 1779
QPLYNEDET+VVTVNGEIYNH ELR+ L HKFKTGSDCDVIAHLYEEHGENFVDMLDGM
Sbjct: 61 QPLYNEDETVVVTVNGEIYNHMELREKLMGHKFKTGSDCDVIAHLYEEHGENFVDMLDGM 120
Query: 1778 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSIWIASELKGLNDDCEHFEAFPPGHLY 1599
FSFVLL TRDNS++VARDAIGITSLYIGWGLDGS+ I+S LKGLNDDCEHFE F GHLY
Sbjct: 121 FSFVLLGTRDNSYLVARDAIGITSLYIGWGLDGSVLISSGLKGLNDDCEHFEVFLAGHLY 180
Query: 1598 S 1596
S
Sbjct: 181 S 181
>ref|XP_002299991.1| predicted protein [Populus trichocarpa] gi|222847249|gb|EEE84796.1|
predicted protein [Populus trichocarpa]
Length = 587
Score = 551 bits (1419), Expect(2) = 0.0
Identities = 277/379 (73%), Positives = 301/379 (79%), Gaps = 7/379 (1%)
Frame = -3
Query: 1497 RQAFENAVIKRLMTDVPFXXXXXXXXXXXXXXSITARHLAGTKAAKQWGAQLHSFCVGLE 1318
R+AFE AVIKRLMTDVPF ++TARHLAGT+AA+QWGA LHSFCVGLE
Sbjct: 211 RRAFEKAVIKRLMTDVPFGVLLSGGLDSSLVAAVTARHLAGTQAARQWGAHLHSFCVGLE 270
Query: 1317 GSPDLKAGKEVADYLGTVHHEFHFTVQDGIDAIEDVIYHIETYDVTTIRASTPMFLMSRK 1138
SPDLKA +EVADYLGT+HHEFHFTVQDGIDAIEDVIYH+ETYDVTTIRASTPMFL++RK
Sbjct: 271 NSPDLKAAREVADYLGTIHHEFHFTVQDGIDAIEDVIYHVETYDVTTIRASTPMFLLARK 330
Query: 1137 IKSLGVKMVISGEGSDEIFGGYLYFHKAPNKEEFHRETCHKIKALHQYDCLRANKSTSAW 958
IK+LGVKMVISGEGSDEIFGGYLYFHKAPNKEE H ETC KIKALHQYDCLRANK+TSAW
Sbjct: 331 IKALGVKMVISGEGSDEIFGGYLYFHKAPNKEELHGETCRKIKALHQYDCLRANKATSAW 390
Query: 957 GLEARVPFLDKEFINVAMSIDPEWKMIKPEEKRIEKWILRKAFDDEERPYLPKHILYRQK 778
GLEARVPFLDK+FINVAM+IDPEWKMIKP RIEKW+LRKAFDDEE PYLPKHILYRQK
Sbjct: 391 GLEARVPFLDKDFINVAMAIDPEWKMIKP--GRIEKWVLRKAFDDEEHPYLPKHILYRQK 448
Query: 777 EQFSDGVGYSWI-------XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXERFFPQNS 619
EQFSDGVGYSWI ERFFPQNS
Sbjct: 449 EQFSDGVGYSWIDGLKAHAELHVHDKMMQNAEHIFPHNTPTTKEAYYYRMIFERFFPQNS 508
Query: 618 ARLTVPGGPSIACSTAKAVEWDASWSNNLDPSGRSALGVHNSAYEKQVASTVNKNLGTQI 439
ARLTVPGG S+ACSTAKAVEWDASWSNNLDPSGR+ALGVH SAYE+Q A + +I
Sbjct: 509 ARLTVPGGASVACSTAKAVEWDASWSNNLDPSGRAALGVHLSAYEQQAALASAGVVPPEI 568
Query: 438 IDNVPRMMEVGAPELTIRS 382
IDN+PRMM+VGAP + I+S
Sbjct: 569 IDNLPRMMKVGAPGVAIQS 587
Score = 354 bits (909), Expect(2) = 0.0
Identities = 165/181 (91%), Positives = 176/181 (97%)
Frame = -1
Query: 2138 MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLYQYGDSYLAHQRLAIIDPASGD 1959
MCGILAVLGCSDDSQAKR RVLELSRRLKHRGPDWSGL+Q+GD YLAHQRLAIIDPASGD
Sbjct: 1 MCGILAVLGCSDDSQAKRFRVLELSRRLKHRGPDWSGLFQHGDFYLAHQRLAIIDPASGD 60
Query: 1958 QPLYNEDETIVVTVNGEIYNHEELRKSLPNHKFKTGSDCDVIAHLYEEHGENFVDMLDGM 1779
QPL+NED+ IVVTVNGEIYNHEELRK LPNHKF+TGSDCDVI+HLYEE+GENFVDMLDGM
Sbjct: 61 QPLFNEDQAIVVTVNGEIYNHEELRKRLPNHKFRTGSDCDVISHLYEEYGENFVDMLDGM 120
Query: 1778 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSIWIASELKGLNDDCEHFEAFPPGHLY 1599
FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGS+WI+SELKGLNDDCEHF+ FPPGH+Y
Sbjct: 121 FSFVLLDTRDNSFIVARDAIGITSLYIGWGLDGSVWISSELKGLNDDCEHFKCFPPGHIY 180
Query: 1598 S 1596
S
Sbjct: 181 S 181