BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002990
         (1666 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q42954.1|KPYC_TOBAC RecName: Full=Pyruvate kinase, cytosolic ...   457   0.0  
ref|XP_002523396.1| pyruvate kinase, putative [Ricinus communis]...   451   0.0  
ref|XP_004149636.1| PREDICTED: pyruvate kinase, cytosolic isozym...   453   0.0  
ref|XP_002304847.1| predicted protein [Populus trichocarpa] gi|2...   451   0.0  
ref|XP_002285763.2| PREDICTED: pyruvate kinase, cytosolic isozym...   450   0.0  

>sp|Q42954.1|KPYC_TOBAC RecName: Full=Pyruvate kinase, cytosolic isozyme; Short=PK
           gi|444023|emb|CAA82628.1| pyruvate kinase [Nicotiana
           tabacum]
          Length = 508

 Score =  457 bits (1175), Expect(2) = 0.0
 Identities = 226/247 (91%), Positives = 237/247 (95%)
 Frame = +2

Query: 2   VEKLLRAGMNVARFNFSHGSHEYHQETLDNLRTAMDNTGILCAVMLDTKGPEIRTGFLKD 181
           +EKLLRAGMNVARFNFSHGSH+YHQET+DNLR AM++TGILCAVMLDTKGPEIRTGFLKD
Sbjct: 36  IEKLLRAGMNVARFNFSHGSHDYHQETIDNLRQAMESTGILCAVMLDTKGPEIRTGFLKD 95

Query: 182 GKPIQLKQGQEITISTDYSIKGDENMICMSYKKLAVDVKPQSVILCADGTISFTVLSCDK 361
            KP+QLKQGQEITISTDYSIKGDE+MICMSYKKLA DVKPQSVILCADG I+FTVLSCDK
Sbjct: 96  AKPVQLKQGQEITISTDYSIKGDESMICMSYKKLAEDVKPQSVILCADGQITFTVLSCDK 155

Query: 362 ENGTVRCRCENTAVLGERKNVNLPGVIVDLPTLEEKDKDDILKWGVPNKIDMIALSFVRK 541
           ENG  RCRCENTAVLGERKNVNLPGVIVDLPTL +KDKDDIL WGVPN IDMIALSFVRK
Sbjct: 156 ENGLDRCRCENTAVLGERKNVNLPGVIVDLPTLTDKDKDDILNWGVPNHIDMIALSFVRK 215

Query: 542 GSDLVEVRKLLGEHAKNILLMSKVENQEGVANFDEILANSDAFMVARGDLGMEIPIEKIF 721
           GSDLVEVRKLLGEHAKNILLMSKVENQEGVANFD+IL NSDAFMVARGDLGMEIPIEKIF
Sbjct: 216 GSDLVEVRKLLGEHAKNILLMSKVENQEGVANFDDILLNSDAFMVARGDLGMEIPIEKIF 275

Query: 722 LAQKVMV 742
           LAQKVM+
Sbjct: 276 LAQKVMI 282



 Score =  374 bits (961), Expect(2) = 0.0
 Identities = 191/207 (92%), Positives = 200/207 (96%)
 Frame = +1

Query: 859  MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVATMAKICVEAESTINYSD 1038
            MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYP+LAV TMAKIC+EAESTI+Y D
Sbjct: 302  MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPDLAVGTMAKICIEAESTIDYPD 361

Query: 1039 VFKRITANAPVPMSPLESLASSAVRTANASKAVLILVLTRGGSTAKLVAKYRPGMPILSV 1218
            VFKRI +NAPVPMSPLESLASSAVRTAN++KA LILVLTRGGSTAKLVAKYRPGMPILSV
Sbjct: 362  VFKRIMSNAPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGMPILSV 421

Query: 1219 VVPEIKTDSFDWSCSDESPARHSLIFRGLIPVLCAGSAKASHAESTEEALDFALQHAKTK 1398
            VVPEIKTDSFDW+CSDESPARHSLIFRGL+PVL AGSA+ASH ESTEEALDFALQHAKTK
Sbjct: 422  VVPEIKTDSFDWTCSDESPARHSLIFRGLVPVLHAGSARASHEESTEEALDFALQHAKTK 481

Query: 1399 GLCKVGDSVVALHRVGTASVIKIVTVK 1479
            GLCK GDSVVALHRVGTASVIKIVTVK
Sbjct: 482  GLCKQGDSVVALHRVGTASVIKIVTVK 508


>ref|XP_002523396.1| pyruvate kinase, putative [Ricinus communis]
           gi|223537346|gb|EEF38975.1| pyruvate kinase, putative
           [Ricinus communis]
          Length = 509

 Score =  451 bits (1159), Expect(2) = 0.0
 Identities = 226/247 (91%), Positives = 235/247 (95%)
 Frame = +2

Query: 2   VEKLLRAGMNVARFNFSHGSHEYHQETLDNLRTAMDNTGILCAVMLDTKGPEIRTGFLKD 181
           +EKLLRAGMNVARFNFSHGSHEYHQETLDNLR AM NTGILCAVMLDTKGPEIRTGFLKD
Sbjct: 37  LEKLLRAGMNVARFNFSHGSHEYHQETLDNLRAAMVNTGILCAVMLDTKGPEIRTGFLKD 96

Query: 182 GKPIQLKQGQEITISTDYSIKGDENMICMSYKKLAVDVKPQSVILCADGTISFTVLSCDK 361
            KPIQLKQGQEITISTDYSIKG+E +ICMSYKKLA DVKP  VILCADGTISFTVLSCD 
Sbjct: 97  AKPIQLKQGQEITISTDYSIKGNEKLICMSYKKLAEDVKPGMVILCADGTISFTVLSCDT 156

Query: 362 ENGTVRCRCENTAVLGERKNVNLPGVIVDLPTLEEKDKDDILKWGVPNKIDMIALSFVRK 541
           + G VRCRCEN+AVLGERKNVNLPGVIVDLPTL EKDK+DILKWGVPN+IDMIALSFVRK
Sbjct: 157 KAGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEKDKEDILKWGVPNQIDMIALSFVRK 216

Query: 542 GSDLVEVRKLLGEHAKNILLMSKVENQEGVANFDEILANSDAFMVARGDLGMEIPIEKIF 721
           GSDLVEVRKLLG HAKNILLMSKVENQEGVANFD+ILANSDAFMVARGDLGMEIPIEKIF
Sbjct: 217 GSDLVEVRKLLGHHAKNILLMSKVENQEGVANFDDILANSDAFMVARGDLGMEIPIEKIF 276

Query: 722 LAQKVMV 742
           LAQKVM+
Sbjct: 277 LAQKVMI 283



 Score =  362 bits (929), Expect(2) = 0.0
 Identities = 182/207 (87%), Positives = 198/207 (95%)
 Frame = +1

Query: 859  MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVATMAKICVEAESTINYSD 1038
            MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAV TMAKIC+EAEST++Y D
Sbjct: 303  MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICLEAESTLDYGD 362

Query: 1039 VFKRITANAPVPMSPLESLASSAVRTANASKAVLILVLTRGGSTAKLVAKYRPGMPILSV 1218
            VFKR+  ++PVPMSPLESLASSAVRTAN++KA LILVLTRGGSTAKLVAKYRPG PILSV
Sbjct: 363  VFKRVMQHSPVPMSPLESLASSAVRTANSAKASLILVLTRGGSTAKLVAKYRPGKPILSV 422

Query: 1219 VVPEIKTDSFDWSCSDESPARHSLIFRGLIPVLCAGSAKASHAESTEEALDFALQHAKTK 1398
            VVPEIKTDSFDWSCS+E+PARHSLIFRGL+PVL AGSA+ASHAE+TEEALDFA+QHAK K
Sbjct: 423  VVPEIKTDSFDWSCSNEAPARHSLIFRGLVPVLYAGSARASHAETTEEALDFAIQHAKAK 482

Query: 1399 GLCKVGDSVVALHRVGTASVIKIVTVK 1479
            GLCK+GDSVVALHRVGTASVIKI+ VK
Sbjct: 483  GLCKIGDSVVALHRVGTASVIKIIYVK 509


>ref|XP_004149636.1| PREDICTED: pyruvate kinase, cytosolic isozyme-like [Cucumis
           sativus] gi|449519038|ref|XP_004166542.1| PREDICTED:
           pyruvate kinase, cytosolic isozyme-like [Cucumis
           sativus]
          Length = 500

 Score =  453 bits (1166), Expect(2) = 0.0
 Identities = 224/247 (90%), Positives = 239/247 (96%)
 Frame = +2

Query: 2   VEKLLRAGMNVARFNFSHGSHEYHQETLDNLRTAMDNTGILCAVMLDTKGPEIRTGFLKD 181
           +EKLL+AGMNVARFNFSHGSH YHQETLDNLR  M+NTGILCAVMLDTKGPEIRTGFLKD
Sbjct: 28  IEKLLKAGMNVARFNFSHGSHAYHQETLDNLRAGMENTGILCAVMLDTKGPEIRTGFLKD 87

Query: 182 GKPIQLKQGQEITISTDYSIKGDENMICMSYKKLAVDVKPQSVILCADGTISFTVLSCDK 361
           GKPIQLKQGQEITISTDYS+KGDENMICMSYKKLA DVKP SVILC+DGTISF+VLSCDK
Sbjct: 88  GKPIQLKQGQEITISTDYSLKGDENMICMSYKKLAEDVKPGSVILCSDGTISFSVLSCDK 147

Query: 362 ENGTVRCRCENTAVLGERKNVNLPGVIVDLPTLEEKDKDDILKWGVPNKIDMIALSFVRK 541
           + G V+CRCEN+AVLGERKNVNLPGVIVDLPTL EKDK+DIL+WGVPNKIDMIALSFVRK
Sbjct: 148 KLGLVQCRCENSAVLGERKNVNLPGVIVDLPTLTEKDKEDILEWGVPNKIDMIALSFVRK 207

Query: 542 GSDLVEVRKLLGEHAKNILLMSKVENQEGVANFDEILANSDAFMVARGDLGMEIPIEKIF 721
           GSDLVEVRKLLG+HAK+ILLMSKVENQEGVANFD+ILANSDAFMVARGDLGMEIPIEKIF
Sbjct: 208 GSDLVEVRKLLGKHAKSILLMSKVENQEGVANFDDILANSDAFMVARGDLGMEIPIEKIF 267

Query: 722 LAQKVMV 742
           LAQKVM+
Sbjct: 268 LAQKVMI 274



 Score =  359 bits (922), Expect(2) = 0.0
 Identities = 181/207 (87%), Positives = 197/207 (95%)
 Frame = +1

Query: 859  MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVATMAKICVEAESTINYSD 1038
            MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAV TMAKICVEAEST++Y D
Sbjct: 294  MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICVEAESTLDYGD 353

Query: 1039 VFKRITANAPVPMSPLESLASSAVRTANASKAVLILVLTRGGSTAKLVAKYRPGMPILSV 1218
            VFKRI  ++PVPMSPLESLASSAVRTAN++KA LILVLTRGGSTAKLVAKYRPG PILSV
Sbjct: 354  VFKRIMEHSPVPMSPLESLASSAVRTANSAKAALILVLTRGGSTAKLVAKYRPGTPILSV 413

Query: 1219 VVPEIKTDSFDWSCSDESPARHSLIFRGLIPVLCAGSAKASHAESTEEALDFALQHAKTK 1398
            VVPEIKTDSFDWSCSDE+PARHSLIFRGL+PVL   SA++SHAE+TEEA++FA+QHAK+K
Sbjct: 414  VVPEIKTDSFDWSCSDEAPARHSLIFRGLVPVLSTASARSSHAETTEEAIEFAIQHAKSK 473

Query: 1399 GLCKVGDSVVALHRVGTASVIKIVTVK 1479
            GLCK GDSVVALHRVGTASVIKI+TVK
Sbjct: 474  GLCKNGDSVVALHRVGTASVIKILTVK 500


>ref|XP_002304847.1| predicted protein [Populus trichocarpa] gi|222842279|gb|EEE79826.1|
           predicted protein [Populus trichocarpa]
          Length = 495

 Score =  451 bits (1159), Expect(2) = 0.0
 Identities = 224/247 (90%), Positives = 235/247 (95%)
 Frame = +2

Query: 2   VEKLLRAGMNVARFNFSHGSHEYHQETLDNLRTAMDNTGILCAVMLDTKGPEIRTGFLKD 181
           +EKLL+AGMNVARFNFSHGSH+YHQETLDNL  AM NTGILCAVMLDTKGPEIRTGFLKD
Sbjct: 23  IEKLLKAGMNVARFNFSHGSHDYHQETLDNLMAAMVNTGILCAVMLDTKGPEIRTGFLKD 82

Query: 182 GKPIQLKQGQEITISTDYSIKGDENMICMSYKKLAVDVKPQSVILCADGTISFTVLSCDK 361
           GKPIQL QGQEITISTDYS+KGDENMICMSYKKLA DV+P  VILCADGT+SFTVLSCD 
Sbjct: 83  GKPIQLNQGQEITISTDYSLKGDENMICMSYKKLAEDVQPGMVILCADGTLSFTVLSCDT 142

Query: 362 ENGTVRCRCENTAVLGERKNVNLPGVIVDLPTLEEKDKDDILKWGVPNKIDMIALSFVRK 541
           E G VRCRCEN+AVLGERKNVNLPGVIVDLPTL EKDK+DIL WGVPNKIDMIALSFVRK
Sbjct: 143 EAGLVRCRCENSAVLGERKNVNLPGVIVDLPTLTEKDKEDILAWGVPNKIDMIALSFVRK 202

Query: 542 GSDLVEVRKLLGEHAKNILLMSKVENQEGVANFDEILANSDAFMVARGDLGMEIPIEKIF 721
           GSDLVEVRKLLG+HAKNILLMSKVENQEGVANFD+ILANSDAFMVARGDLGMEIPIEKIF
Sbjct: 203 GSDLVEVRKLLGKHAKNILLMSKVENQEGVANFDDILANSDAFMVARGDLGMEIPIEKIF 262

Query: 722 LAQKVMV 742
           LAQKVM+
Sbjct: 263 LAQKVMI 269



 Score =  362 bits (929), Expect(2) = 0.0
 Identities = 184/207 (88%), Positives = 196/207 (94%)
 Frame = +1

Query: 859  MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVATMAKICVEAESTINYSD 1038
            MIK+PRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAV TMAKICVEAEST++Y D
Sbjct: 289  MIKAPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVQTMAKICVEAESTLDYGD 348

Query: 1039 VFKRITANAPVPMSPLESLASSAVRTANASKAVLILVLTRGGSTAKLVAKYRPGMPILSV 1218
            VFKR T ++PVPMSPLESLASSAVRTAN+ KA LILVLTRGGSTAKLVAKYRPGMPILSV
Sbjct: 349  VFKRTTKHSPVPMSPLESLASSAVRTANSVKASLILVLTRGGSTAKLVAKYRPGMPILSV 408

Query: 1219 VVPEIKTDSFDWSCSDESPARHSLIFRGLIPVLCAGSAKASHAESTEEALDFALQHAKTK 1398
            VVPEI+TDSFDWSCSDE+PARHSLIFRGL+PVL AGSAKASHAE+TEEALDFALQHAK K
Sbjct: 409  VVPEIQTDSFDWSCSDEAPARHSLIFRGLVPVLYAGSAKASHAETTEEALDFALQHAKGK 468

Query: 1399 GLCKVGDSVVALHRVGTASVIKIVTVK 1479
            GLC+ GD VVALHRVGTASVIKI+ VK
Sbjct: 469  GLCRTGDPVVALHRVGTASVIKIINVK 495


>ref|XP_002285763.2| PREDICTED: pyruvate kinase, cytosolic isozyme-like [Vitis vinifera]
          Length = 506

 Score =  450 bits (1157), Expect(2) = 0.0
 Identities = 226/247 (91%), Positives = 234/247 (94%)
 Frame = +2

Query: 2   VEKLLRAGMNVARFNFSHGSHEYHQETLDNLRTAMDNTGILCAVMLDTKGPEIRTGFLKD 181
           +EKLLRAGMNVARFNFSHGSHEYH ETL NLR AMD+TGILCAVMLDTKGPEIRTGFLKD
Sbjct: 34  LEKLLRAGMNVARFNFSHGSHEYHLETLSNLRAAMDSTGILCAVMLDTKGPEIRTGFLKD 93

Query: 182 GKPIQLKQGQEITISTDYSIKGDENMICMSYKKLAVDVKPQSVILCADGTISFTVLSCDK 361
            KPI LKQGQEITISTDY+IKGDE MICMSYKKLA DVKP SVILCADGTI+FTVLSCDK
Sbjct: 94  EKPIHLKQGQEITISTDYNIKGDEKMICMSYKKLAEDVKPDSVILCADGTITFTVLSCDK 153

Query: 362 ENGTVRCRCENTAVLGERKNVNLPGVIVDLPTLEEKDKDDILKWGVPNKIDMIALSFVRK 541
           + G V CRCEN+AVLGERKNVNLPGVIVDLPTL EKDK+DIL WGVPNKIDMIALSFVRK
Sbjct: 154 QKGLVCCRCENSAVLGERKNVNLPGVIVDLPTLTEKDKEDILNWGVPNKIDMIALSFVRK 213

Query: 542 GSDLVEVRKLLGEHAKNILLMSKVENQEGVANFDEILANSDAFMVARGDLGMEIPIEKIF 721
           GSDLVEVRKLLG+HAKNILLMSKVENQEGVANFDEILANSDAFMVARGDLGMEIPIEKIF
Sbjct: 214 GSDLVEVRKLLGKHAKNILLMSKVENQEGVANFDEILANSDAFMVARGDLGMEIPIEKIF 273

Query: 722 LAQKVMV 742
           LAQKVMV
Sbjct: 274 LAQKVMV 280



 Score =  361 bits (927), Expect(2) = 0.0
 Identities = 181/207 (87%), Positives = 197/207 (95%)
 Frame = +1

Query: 859  MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVATMAKICVEAESTINYSD 1038
            MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAV TMAKIC+EAEST++Y D
Sbjct: 300  MIKSPRPTRAEATDVANAVLDGTDCVMLSGETAAGAYPELAVRTMAKICIEAESTLDYGD 359

Query: 1039 VFKRITANAPVPMSPLESLASSAVRTANASKAVLILVLTRGGSTAKLVAKYRPGMPILSV 1218
            VFKRI  NAPVPMSPLESLA+SAVRTAN+++A LILVLTRGGSTAKLVAKYRPGMPILSV
Sbjct: 360  VFKRIMKNAPVPMSPLESLAASAVRTANSARAALILVLTRGGSTAKLVAKYRPGMPILSV 419

Query: 1219 VVPEIKTDSFDWSCSDESPARHSLIFRGLIPVLCAGSAKASHAESTEEALDFALQHAKTK 1398
            VVPEIKTDSFDWSCSDE+PARHSLIFRGL+PVL A SA+ASHAE+TEEAL+FA+QHAK K
Sbjct: 420  VVPEIKTDSFDWSCSDEAPARHSLIFRGLVPVLSAASARASHAETTEEALEFAIQHAKAK 479

Query: 1399 GLCKVGDSVVALHRVGTASVIKIVTVK 1479
            G CK GDS+VALHRVG+ASVIKI+TVK
Sbjct: 480  GFCKKGDSLVALHRVGSASVIKILTVK 506