BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002963
         (1104 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAS60194.1| hexokinase 3 [Nicotiana tabacum]                       565   e-159
gb|AAP40021.1| hexokinase [Nicotiana benthamiana]                     564   e-158
sp|Q9SEK2.1|HXK1_TOBAC RecName: Full=Hexokinase-1; AltName: Full...   559   e-157
gb|AAS60192.1| hexokinase 1a [Nicotiana tabacum]                      557   e-156
gb|AAT77511.1| hexokinase [Nicotiana sylvestris]                      557   e-156

>gb|AAS60194.1| hexokinase 3 [Nicotiana tabacum]
          Length = 497

 Score =  565 bits (1457), Expect = e-159
 Identities = 279/343 (81%), Positives = 313/343 (91%), Gaps = 2/343 (0%)
 Frame = -2

Query: 1025 RNQLRTSRQCARAAAILKEFEEKCGTPSSKLKQVADAMTVEMHAGLASEGGSKLKMLISY 846
            R+++R S + ARA AILKEFEEKCGTP +KLKQVADAMTVEMHAGLASEGGSKLKMLISY
Sbjct: 25   RHRMRKSSKWARARAILKEFEEKCGTPDAKLKQVADAMTVEMHAGLASEGGSKLKMLISY 84

Query: 845  VDNLPSGAEVGVYYALDLGGTNFRVLRVQLG--NGGVVDQEFSEVSIPPNLMVGTSQELF 672
            VDNLP+G E GV+YALDLGGTNFRVLRVQLG  +GG+V QEF+E SIPPNLMVGTS+ LF
Sbjct: 85   VDNLPTGDEEGVFYALDLGGTNFRVLRVQLGGKDGGIVHQEFTEASIPPNLMVGTSEALF 144

Query: 671  DYIAAELAKFVAQEGDRFQLPTGRKRELGFTFSFPVMQNSIVSGTIIRWTKGFSIDDAVG 492
            DYIAAELAKFVA+E ++F  P G++RELGFTFSFP+MQ SI SGT+IRWTKGFSIDD VG
Sbjct: 145  DYIAAELAKFVAEEEEKFHQPPGKQRELGFTFSFPIMQTSINSGTLIRWTKGFSIDDTVG 204

Query: 491  KDVVAELSSAMERQGVEMHVSALVNDTVGTLAGGRYSNKDVVVAVIMGTGTNAAYVERAQ 312
            +DVVAEL+ AM+R+GV+M VSALVNDTVGTLAGGR+SNKDV +AVI+GTGTNAAYVERAQ
Sbjct: 205  QDVVAELTKAMQRKGVDMRVSALVNDTVGTLAGGRFSNKDVSIAVILGTGTNAAYVERAQ 264

Query: 311  AIPKWHGPQPESGEMVINMEWGNFRSSHLPLTEYDHALDAESLNPGEQIFEKITSGMYLG 132
            AIPKWHGP P+SGEMVINMEWGNFRSSHLPLTEYDHA+D +SLNPGEQIFEKI SGMYLG
Sbjct: 265  AIPKWHGPLPKSGEMVINMEWGNFRSSHLPLTEYDHAMDTDSLNPGEQIFEKICSGMYLG 324

Query: 131  EIVRRVLCRMAEESALFGDTCPPKLKTPFILRTPDMSAMHHDT 3
            EI+RRVL RM+EE+A+FGD  PPKLK  FILRTP+MSAMHHDT
Sbjct: 325  EILRRVLLRMSEEAAIFGDEVPPKLKNQFILRTPEMSAMHHDT 367


>gb|AAP40021.1| hexokinase [Nicotiana benthamiana]
          Length = 497

 Score =  564 bits (1454), Expect = e-158
 Identities = 275/342 (80%), Positives = 313/342 (91%), Gaps = 2/342 (0%)
 Frame = -2

Query: 1022 NQLRTSRQCARAAAILKEFEEKCGTPSSKLKQVADAMTVEMHAGLASEGGSKLKMLISYV 843
            +++R S + ARA AIL+EFEEKCGTP +KLKQVADAMTVEMHAGLASEGGSKLKMLI+YV
Sbjct: 26   HRMRKSSKWARAMAILREFEEKCGTPDAKLKQVADAMTVEMHAGLASEGGSKLKMLITYV 85

Query: 842  DNLPSGAEVGVYYALDLGGTNFRVLRVQLG--NGGVVDQEFSEVSIPPNLMVGTSQELFD 669
            DNLP+G E GV+YALDLGGTNFRVLRVQLG  +GG++ QEF+E SIPPNLMVGTS+ELFD
Sbjct: 86   DNLPTGDEAGVFYALDLGGTNFRVLRVQLGGKDGGIIHQEFAEASIPPNLMVGTSEELFD 145

Query: 668  YIAAELAKFVAQEGDRFQLPTGRKRELGFTFSFPVMQNSIVSGTIIRWTKGFSIDDAVGK 489
            YIAAELAKFVA+E ++FQ P G++RELGFTFSFPVMQ SI SGTI+RWTKGFSIDDAVG+
Sbjct: 146  YIAAELAKFVAEEEEKFQQPPGKQRELGFTFSFPVMQTSINSGTIMRWTKGFSIDDAVGQ 205

Query: 488  DVVAELSSAMERQGVEMHVSALVNDTVGTLAGGRYSNKDVVVAVIMGTGTNAAYVERAQA 309
            DVV EL+ AM+R+GV+M VSALVNDTVGTLAGG+Y++ DV VAVI+GTGTNAAYVER QA
Sbjct: 206  DVVGELTKAMKRKGVDMRVSALVNDTVGTLAGGKYTHNDVAVAVILGTGTNAAYVERVQA 265

Query: 308  IPKWHGPQPESGEMVINMEWGNFRSSHLPLTEYDHALDAESLNPGEQIFEKITSGMYLGE 129
            IPKWHGP P+SGEMVINMEWGNFRSSHLPLT+YDHALD  SLNPG+QIFEK+TSGMYLGE
Sbjct: 266  IPKWHGPMPKSGEMVINMEWGNFRSSHLPLTQYDHALDTNSLNPGDQIFEKMTSGMYLGE 325

Query: 128  IVRRVLCRMAEESALFGDTCPPKLKTPFILRTPDMSAMHHDT 3
            I+RRVL RMAEE+ +FGD  PPKLK+PF+LRTPDMSAMHHDT
Sbjct: 326  ILRRVLLRMAEEAGIFGDEVPPKLKSPFVLRTPDMSAMHHDT 367


>sp|Q9SEK2.1|HXK1_TOBAC RecName: Full=Hexokinase-1; AltName: Full=NtHxK1
            gi|6594674|gb|AAF18585.1|AF118133_1 chloroplast outer
            envelope hexokinase 1 [Nicotiana tabacum]
          Length = 497

 Score =  559 bits (1440), Expect = e-157
 Identities = 273/341 (80%), Positives = 311/341 (91%), Gaps = 2/341 (0%)
 Frame = -2

Query: 1022 NQLRTSRQCARAAAILKEFEEKCGTPSSKLKQVADAMTVEMHAGLASEGGSKLKMLISYV 843
            +++R S + ARA AIL+EFEEKCGTP +KLKQVADAMTVEMHAGLASEGGSKLKMLI+YV
Sbjct: 26   HRMRKSSKWARAMAILREFEEKCGTPDAKLKQVADAMTVEMHAGLASEGGSKLKMLITYV 85

Query: 842  DNLPSGAEVGVYYALDLGGTNFRVLRVQLG--NGGVVDQEFSEVSIPPNLMVGTSQELFD 669
            DNLP+G E GV+YALDLGGTNFRVLRVQLG  +GG+V QEF+E SIPPNLMVGTS+ LFD
Sbjct: 86   DNLPTGDEAGVFYALDLGGTNFRVLRVQLGGKDGGIVHQEFAEASIPPNLMVGTSEALFD 145

Query: 668  YIAAELAKFVAQEGDRFQLPTGRKRELGFTFSFPVMQNSIVSGTIIRWTKGFSIDDAVGK 489
            YIAAELAKFV +EG++FQ P G++RELGFTFSFPVMQ SI SGTI+RWTKGFSIDDAVG+
Sbjct: 146  YIAAELAKFVNEEGEKFQQPPGKQRELGFTFSFPVMQTSINSGTIMRWTKGFSIDDAVGQ 205

Query: 488  DVVAELSSAMERQGVEMHVSALVNDTVGTLAGGRYSNKDVVVAVIMGTGTNAAYVERAQA 309
            DVV EL+ AM+R+GV+M VSALVNDTVGTLAGG+Y++ DV VAVI+GTGTNAAYVER QA
Sbjct: 206  DVVGELAKAMKRKGVDMRVSALVNDTVGTLAGGKYTHNDVAVAVILGTGTNAAYVERVQA 265

Query: 308  IPKWHGPQPESGEMVINMEWGNFRSSHLPLTEYDHALDAESLNPGEQIFEKITSGMYLGE 129
            IPKWHGP P+SGEMVINMEWGNFRSSHLPLT+YDHALD  SLNPG+QIFEK+TSGMYLGE
Sbjct: 266  IPKWHGPVPKSGEMVINMEWGNFRSSHLPLTQYDHALDTNSLNPGDQIFEKMTSGMYLGE 325

Query: 128  IVRRVLCRMAEESALFGDTCPPKLKTPFILRTPDMSAMHHD 6
            I+RRVL R+AEE+ +FGD  PPKLK+PF+LRTPDMSAMHHD
Sbjct: 326  ILRRVLLRVAEEAGIFGDEVPPKLKSPFVLRTPDMSAMHHD 366


>gb|AAS60192.1| hexokinase 1a [Nicotiana tabacum]
          Length = 497

 Score =  557 bits (1435), Expect = e-156
 Identities = 272/341 (79%), Positives = 311/341 (91%), Gaps = 2/341 (0%)
 Frame = -2

Query: 1022 NQLRTSRQCARAAAILKEFEEKCGTPSSKLKQVADAMTVEMHAGLASEGGSKLKMLISYV 843
            +++R S + ARA AIL+EFEEKCGTP +KLKQVADAMTVEMHAGLASEGGSKLKMLI+YV
Sbjct: 26   HRMRKSSKWARAMAILREFEEKCGTPDAKLKQVADAMTVEMHAGLASEGGSKLKMLITYV 85

Query: 842  DNLPSGAEVGVYYALDLGGTNFRVLRVQLG--NGGVVDQEFSEVSIPPNLMVGTSQELFD 669
            DNLP+G E GV+YALDLGGTNFRVLRVQLG  +GG+V QEF+E SIPPNLMVGTS+ LFD
Sbjct: 86   DNLPTGDEAGVFYALDLGGTNFRVLRVQLGGKDGGIVHQEFAEASIPPNLMVGTSEALFD 145

Query: 668  YIAAELAKFVAQEGDRFQLPTGRKRELGFTFSFPVMQNSIVSGTIIRWTKGFSIDDAVGK 489
            YIAAELAKFVA+E ++FQ P G++RELGFTFSFPVMQ SI SGTI+RWTKGFSIDDAVG+
Sbjct: 146  YIAAELAKFVAEEEEKFQQPPGKQRELGFTFSFPVMQTSINSGTIMRWTKGFSIDDAVGQ 205

Query: 488  DVVAELSSAMERQGVEMHVSALVNDTVGTLAGGRYSNKDVVVAVIMGTGTNAAYVERAQA 309
            DVV EL+ AM+R+GV+M VSALVNDTVGTLAGG+Y++ DV VAVI+GTGTNAAYVER QA
Sbjct: 206  DVVGELTKAMKRKGVDMRVSALVNDTVGTLAGGKYTHNDVAVAVILGTGTNAAYVERVQA 265

Query: 308  IPKWHGPQPESGEMVINMEWGNFRSSHLPLTEYDHALDAESLNPGEQIFEKITSGMYLGE 129
            IPKWHGP P+SGEMVINMEWGNFRSSHLPLT+YDHALD  SLNPG+QIFEK+TSGMYLGE
Sbjct: 266  IPKWHGPVPKSGEMVINMEWGNFRSSHLPLTQYDHALDTNSLNPGDQIFEKMTSGMYLGE 325

Query: 128  IVRRVLCRMAEESALFGDTCPPKLKTPFILRTPDMSAMHHD 6
            I+RRVL R+AE++ +FGD  PPKLK+PF+LRTPDMSAMHHD
Sbjct: 326  ILRRVLLRVAEDAGIFGDEVPPKLKSPFVLRTPDMSAMHHD 366


>gb|AAT77511.1| hexokinase [Nicotiana sylvestris]
          Length = 383

 Score =  557 bits (1435), Expect = e-156
 Identities = 272/341 (79%), Positives = 311/341 (91%), Gaps = 2/341 (0%)
 Frame = -2

Query: 1022 NQLRTSRQCARAAAILKEFEEKCGTPSSKLKQVADAMTVEMHAGLASEGGSKLKMLISYV 843
            +++R S + ARA AIL+EFEEKCGTP +KLKQVADAMTVEMHAGLASEGGSKLKMLI+YV
Sbjct: 26   HRMRKSSKWARAMAILREFEEKCGTPDAKLKQVADAMTVEMHAGLASEGGSKLKMLITYV 85

Query: 842  DNLPSGAEVGVYYALDLGGTNFRVLRVQLG--NGGVVDQEFSEVSIPPNLMVGTSQELFD 669
            DNLP+G E GV+YALDLGGTNFRVLRVQLG  +GG+V QEF+E SIPPNLMVGTS+ LFD
Sbjct: 86   DNLPTGDEAGVFYALDLGGTNFRVLRVQLGGKDGGIVHQEFAEASIPPNLMVGTSEALFD 145

Query: 668  YIAAELAKFVAQEGDRFQLPTGRKRELGFTFSFPVMQNSIVSGTIIRWTKGFSIDDAVGK 489
            YIAAELAKFVA+E ++FQ P G++RELGFTFSFPVMQ SI SGTI+RWTKGFSIDDAVG+
Sbjct: 146  YIAAELAKFVAEEEEKFQQPPGKQRELGFTFSFPVMQTSINSGTIMRWTKGFSIDDAVGQ 205

Query: 488  DVVAELSSAMERQGVEMHVSALVNDTVGTLAGGRYSNKDVVVAVIMGTGTNAAYVERAQA 309
            DVV EL+ AM+R+GV+M VSALVNDTVGTLAGG+Y++ DV VAVI+GTGTNAAYVER QA
Sbjct: 206  DVVGELTKAMKRKGVDMRVSALVNDTVGTLAGGKYTHNDVAVAVILGTGTNAAYVERVQA 265

Query: 308  IPKWHGPQPESGEMVINMEWGNFRSSHLPLTEYDHALDAESLNPGEQIFEKITSGMYLGE 129
            IPKWHGP P+SGEMVINMEWGNFRSSHLPLT+YDHALD  SLNPG+QIFEK+TSGMYLGE
Sbjct: 266  IPKWHGPVPKSGEMVINMEWGNFRSSHLPLTQYDHALDTNSLNPGDQIFEKMTSGMYLGE 325

Query: 128  IVRRVLCRMAEESALFGDTCPPKLKTPFILRTPDMSAMHHD 6
            I+RRVL R+AE++ +FGD  PPKLK+PF+LRTPDMSAMHHD
Sbjct: 326  ILRRVLLRVAEDAGIFGDEVPPKLKSPFVLRTPDMSAMHHD 366