BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002880
         (3548 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002276207.1| PREDICTED: isoleucyl-tRNA synthetase-like [V...  1691   0.0  
ref|XP_002516921.1| isoleucyl tRNA synthetase, putative [Ricinus...  1649   0.0  
ref|NP_001190497.1| isoleucyl-tRNA synthetase [Arabidopsis thali...  1637   0.0  
ref|NP_199714.2| isoleucyl-tRNA synthetase [Arabidopsis thaliana...  1637   0.0  
ref|XP_004134489.1| PREDICTED: isoleucine--tRNA ligase-like [Cuc...  1625   0.0  

>ref|XP_002276207.1| PREDICTED: isoleucyl-tRNA synthetase-like [Vitis vinifera]
          Length = 1105

 Score = 1691 bits (4378), Expect = 0.0
 Identities = 817/1062 (76%), Positives = 914/1062 (86%), Gaps = 3/1062 (0%)
 Frame = -3

Query: 3435 QMSSYKAMSLRSFSSFRKAPSTTIFNFRRSSSAIFLAFLDITCYSTISNNEFHPSSKRRS 3256
            ++S    +S R+ SSFR   S ++   R SSS    + L    YS+ S++    SSKRRS
Sbjct: 36   RVSVLHVLSQRTASSFRSMNSVSLLYLRGSSSVHVPSLLKTATYSSYSSDNSSSSSKRRS 95

Query: 3255 RGPVMAAKKASKGEKLEDGKYKHTVDLPKTNFGMRANSLTREPEIQKLWEDNQVFKRLTD 3076
            RGPVMAAKKAS+  K EDG+YKHTVDLPKT FGMRANS +REPEIQKLW+DNQVFKR+ D
Sbjct: 96   RGPVMAAKKASEAAKQEDGRYKHTVDLPKTAFGMRANSTSREPEIQKLWDDNQVFKRVVD 155

Query: 3075 RNTGGNFVLHDGPPYANGDLHMGHALNKILKDMINRYKLLQNFKVHYVPGWDCHGLPIEL 2896
            RN GGNF+LHDGPPYANGDLHMGHALNKILKD+INRYKLLQN+KVHYVPGWDCHGLPIEL
Sbjct: 156  RNNGGNFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVHYVPGWDCHGLPIEL 215

Query: 2895 KVLQSLDQEARKALTPXXXXXXXXXXXXXXXXAQMSSFKRYGVWADWDHPYLTLDPDYEA 2716
            KVLQSLDQ+AR+ LTP                 QM+SFKRYGVW DW++PYLTLDP+YEA
Sbjct: 216  KVLQSLDQDARRELTPLKLRAKASRFAKATVKNQMASFKRYGVWGDWNNPYLTLDPEYEA 275

Query: 2715 SQIEVFGQMVLQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHVSKSMYAAFRVLHAPTS 2536
            SQIEVFGQM LQGYI+RGRKPVHWSPSSRTALAEAELEYPEGH+SKS+YA FR++ AP +
Sbjct: 276  SQIEVFGQMALQGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIYAIFRLVSAPVT 335

Query: 2535 DG--MLEFFPNLFLAIWTTTPWTMPANAAVAVNAKLQYAVVEVHRPLADLSTSSDGKK-L 2365
             G  + +F P+L LAIWTTTPWT+PANAAVAVNAKLQY+VVEVH PL D+S S   +K  
Sbjct: 336  SGTSLEDFLPDLCLAIWTTTPWTIPANAAVAVNAKLQYSVVEVHSPLEDVSKSKQNEKGR 395

Query: 2364 VGNLLKEQEKSFFIVAEDLVSTLEAKWGXXXXXXXXXLGSDLENCRYTHPIEKRECPVVI 2185
            +GN LKEQ+  F +VA DLV TLEAKWG         LGSDLENCRY HPI+KRECPVVI
Sbjct: 396  LGNALKEQKNLFLVVASDLVPTLEAKWGLKLVIKKTLLGSDLENCRYIHPIDKRECPVVI 455

Query: 2184 GGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPVLSPVDDDGKFTEEAGEFSGLDVLGD 2005
            GGDYITTESGTGLVHTAPGHGQEDY TG+KYGLP+LSPVDDDGKFTEEAG+FSGLDVLGD
Sbjct: 456  GGDYITTESGTGLVHTAPGHGQEDYVTGMKYGLPILSPVDDDGKFTEEAGQFSGLDVLGD 515

Query: 2004 GNAAVIKYLDKLSSLIMVEPYTHKYPYDWRTKKPTIFRATEQWFASVEGFRKAAMDAISH 1825
            GNAAV+++LD+  S+IM EPY HKYPYDWRTKKPTIFRATEQWFASVEGFR+ AM AI  
Sbjct: 516  GNAAVVRFLDENLSIIMEEPYKHKYPYDWRTKKPTIFRATEQWFASVEGFRQVAMTAIGQ 575

Query: 1824 VTWTPSQAENRISAMASTRSDWCISRQRTWGVPIPVFYHVETKEPLMNEETISHIKSIIS 1645
            VTW P+QAENRISAM S+RSDWCISRQRTWGVPIPVFYHV++KEPLMN+ETI H+KSI+S
Sbjct: 576  VTWIPAQAENRISAMTSSRSDWCISRQRTWGVPIPVFYHVQSKEPLMNKETIDHVKSIVS 635

Query: 1644 QKGSDAWWYMSVEELLPDKYRGCASEYEKGTDTMDVWFDSGSSWAAVLDKREGLSYPADL 1465
            QKGSDAWWYM+VEELLPDKYR  AS YEKGTDTMDVWFDSGSSWAAVL+ R  LS PADL
Sbjct: 636  QKGSDAWWYMTVEELLPDKYRNKASGYEKGTDTMDVWFDSGSSWAAVLESRNELSCPADL 695

Query: 1464 YLEGTDQHRGWFQSSLLTSVATKGKAPYSGVITHGFVLDERGLKMSKSLGNVVDPITVIE 1285
            YLEGTDQHRGWFQSSLLTSVATKG+APYS VITHGFVLDE+G KMSKSLGNVVDP TVIE
Sbjct: 696  YLEGTDQHRGWFQSSLLTSVATKGRAPYSSVITHGFVLDEKGFKMSKSLGNVVDPRTVIE 755

Query: 1284 GGKNSGEAPSYGADVLRLWVSSVDYTGDVLIGPQVLRQMSDIYRKLRGTLRFLLANLHDW 1105
            GGKN  EAP YGADVLRLWVSSVDYTGD +IG QVLRQMSDIYRKLRGTLR+LLANLHDW
Sbjct: 756  GGKNLKEAPGYGADVLRLWVSSVDYTGDAMIGAQVLRQMSDIYRKLRGTLRYLLANLHDW 815

Query: 1104 EAGYAVPYSDLPVIDQHALFQLEHVVKNIGDSYENYQFFKIFQTIQRFVIVDLSNFYFDV 925
            +A  AVPY DLP+ID+HALFQLE+VVKNI +SYE+YQFFKIFQ IQRF IVDLSNFYFDV
Sbjct: 816  KADNAVPYCDLPMIDRHALFQLENVVKNIRESYESYQFFKIFQIIQRFAIVDLSNFYFDV 875

Query: 924  AKDRLYVGGTASFTRRSCQTVLAEHLLSITRVIAPILPHLAEDVWQHLPFQYAARDGDVA 745
            AKDRLYVGGT SFTRRSCQTVLA HLLSI RVIAPILPHLAEDVWQ+LPFQY   DG +A
Sbjct: 876  AKDRLYVGGTTSFTRRSCQTVLAAHLLSIVRVIAPILPHLAEDVWQNLPFQYTNEDGSIA 935

Query: 744  KFVFESRWPAVNDKWLAFPLKEVEFWGKILELRTEVNRTLEAARTGKLIGSSLEAKVYLY 565
            +FVFESRWPA+N+KWL FP +E++FWGKILELRTE N+ LE AR GKLIGSSL+AKVYL+
Sbjct: 936  EFVFESRWPALNEKWLTFPTEEIDFWGKILELRTETNKVLEVARGGKLIGSSLDAKVYLH 995

Query: 564  SFDDSLSERFKEMCETKNDADALYRIFITSQVEILSSLDNAQVENIPYTGEYVIEGDNKI 385
            + D SL+ R +EMC T NDAD L+RIFITSQVEILSSLD+  V+NIPYTGEY+I+G NKI
Sbjct: 996  ASDASLAPRLQEMCSTNNDADTLHRIFITSQVEILSSLDDELVKNIPYTGEYLIQGKNKI 1055

Query: 384  WIGVSRAEGSKCERCWNFSPQVGSYAEHPSLCSRCFSAIDGQ 259
            WIGVSRA+GSKCERCWN+S QVGS++EHP+LC RC++ ++ Q
Sbjct: 1056 WIGVSRADGSKCERCWNYSLQVGSFSEHPTLCGRCYNVVNVQ 1097


>ref|XP_002516921.1| isoleucyl tRNA synthetase, putative [Ricinus communis]
            gi|223544009|gb|EEF45535.1| isoleucyl tRNA synthetase,
            putative [Ricinus communis]
          Length = 1102

 Score = 1649 bits (4269), Expect = 0.0
 Identities = 797/1072 (74%), Positives = 904/1072 (84%), Gaps = 14/1072 (1%)
 Frame = -3

Query: 3429 SSYKAMSLRSFS-SFRKAPSTTIFNFRRSSSAIFLAFLDITCYSTISNNEFHPSSKRRSR 3253
            SS +A++ RS S S R   +  +F +R SSS   L+FL+   Y T S++EF  SSKRRSR
Sbjct: 24   SSSRALTRRSCSPSLRNVTAVDVFYYRGSSSTKVLSFLNTNRYCTYSSDEFGTSSKRRSR 83

Query: 3252 GPVMAAKKASKGEKLEDGKYKHTVDLPKTNFGMRANSLTREPEIQKLWEDNQVFKRLTDR 3073
            GPVMAAKK+S GEK E+GKYKHTVDLPKT F MRAN+LTREPE+QKLW+DNQVFKR+ D+
Sbjct: 84   GPVMAAKKSSDGEKQEEGKYKHTVDLPKTTFSMRANALTREPELQKLWDDNQVFKRVVDK 143

Query: 3072 NTGGNFVLHDGPPYANGDLHMGHALNKILKDMINRYKLLQNFKVHYVPGWDCHGLPIELK 2893
            N GGNF+LHDGPPYANGDLH+GHA+NKILKD+INRYK+LQN+KVH+VPGWDCHGLPIELK
Sbjct: 144  NDGGNFILHDGPPYANGDLHIGHAMNKILKDVINRYKILQNYKVHFVPGWDCHGLPIELK 203

Query: 2892 ----------VLQSLDQEARKALTPXXXXXXXXXXXXXXXXAQMSSFKRYGVWADWDHPY 2743
                       LQSLDQ AR+ LTP                 QM+SFKRYGVWADWD+PY
Sbjct: 204  GKYKVLFWLRFLQSLDQGAREDLTPSKLRAKAAKFAKATVKTQMASFKRYGVWADWDNPY 263

Query: 2742 LTLDPDYEASQIEVFGQMVLQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHVSKSMYAA 2563
            LTLDPDYEA+QIEVFGQM LQGYI+RGRKPVHWSPSS TALAEAELEYPEGHVSKS+YA 
Sbjct: 264  LTLDPDYEAAQIEVFGQMALQGYIYRGRKPVHWSPSSHTALAEAELEYPEGHVSKSIYAI 323

Query: 2562 FRVLHAPTSDGML--EFFPNLFLAIWTTTPWTMPANAAVAVNAKLQYAVVEVHRPLADLS 2389
            FRV  AP +  +L  EFFP+LFLAIWTTTPWT+PANAAVAVN+KLQYAVVEV + L D S
Sbjct: 324  FRVASAPPTSRLLLEEFFPDLFLAIWTTTPWTVPANAAVAVNSKLQYAVVEV-QSLEDAS 382

Query: 2388 TSSDGKKL-VGNLLKEQEKSFFIVAEDLVSTLEAKWGXXXXXXXXXLGSDLENCRYTHPI 2212
            TS   KK   GN+L+EQ+K F IVA DL+ TLEAKW           GSDLENCRY HPI
Sbjct: 383  TSPGNKKRRFGNVLREQKKLFLIVASDLMPTLEAKWSVKLVIKKTLSGSDLENCRYIHPI 442

Query: 2211 EKRECPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPVLSPVDDDGKFTEEAGE 2032
            + RECPVVIGGDYITTESGTGLVHTAPGHGQEDY TG+KYGLPVLSPVDD GKFTEEA  
Sbjct: 443  DNRECPVVIGGDYITTESGTGLVHTAPGHGQEDYITGMKYGLPVLSPVDDGGKFTEEAAP 502

Query: 2031 FSGLDVLGDGNAAVIKYLDKLSSLIMVEPYTHKYPYDWRTKKPTIFRATEQWFASVEGFR 1852
            FSGLDVLG+GN AV+KYLD+  S++M E Y HKYPYDWRTKKPTIFRATEQWFASVEGFR
Sbjct: 503  FSGLDVLGEGNVAVVKYLDEQMSIVMEESYEHKYPYDWRTKKPTIFRATEQWFASVEGFR 562

Query: 1851 KAAMDAISHVTWTPSQAENRISAMASTRSDWCISRQRTWGVPIPVFYHVETKEPLMNEET 1672
            +AAMDAI HV W P QAE RIS M S+RSDWCISRQRTWGVPIPVFYHV+++EPLMN ET
Sbjct: 563  QAAMDAIGHVKWIPPQAEKRISTMTSSRSDWCISRQRTWGVPIPVFYHVQSREPLMNAET 622

Query: 1671 ISHIKSIISQKGSDAWWYMSVEELLPDKYRGCASEYEKGTDTMDVWFDSGSSWAAVLDKR 1492
            I HIKSI++QKGSDAWWYM+VE LLPD YR  ASEYE+GTDTMDVWFDSGSSWAAVL +R
Sbjct: 623  IDHIKSIVAQKGSDAWWYMTVENLLPDTYRDRASEYERGTDTMDVWFDSGSSWAAVLGRR 682

Query: 1491 EGLSYPADLYLEGTDQHRGWFQSSLLTSVATKGKAPYSGVITHGFVLDERGLKMSKSLGN 1312
             GL+YPADLYLEG+DQHRGWFQSSLLTS+ATKGKAPYS VITHGFVLDE+G KMSKSLGN
Sbjct: 683  SGLNYPADLYLEGSDQHRGWFQSSLLTSIATKGKAPYSSVITHGFVLDEKGFKMSKSLGN 742

Query: 1311 VVDPITVIEGGKNSGEAPSYGADVLRLWVSSVDYTGDVLIGPQVLRQMSDIYRKLRGTLR 1132
            VVDP TVIEGGK++G AP+YGADVLRLWVSSVDYTGDV+IGPQ+LRQMSDIYRKLRGTLR
Sbjct: 743  VVDPRTVIEGGKSAGGAPAYGADVLRLWVSSVDYTGDVMIGPQILRQMSDIYRKLRGTLR 802

Query: 1131 FLLANLHDWEAGYAVPYSDLPVIDQHALFQLEHVVKNIGDSYENYQFFKIFQTIQRFVIV 952
            +LL NLHDW+A  AV Y DLP+ID+HAL+QLE+VVKNI +SYENYQFFKIFQ IQRFVIV
Sbjct: 803  YLLGNLHDWKADDAVSYDDLPMIDKHALYQLENVVKNIRESYENYQFFKIFQIIQRFVIV 862

Query: 951  DLSNFYFDVAKDRLYVGGTASFTRRSCQTVLAEHLLSITRVIAPILPHLAEDVWQHLPFQ 772
            DLSNFYFDVAKDRLYVGGT +FTR+SCQTVLA HLLSI +V+APILPHLAEDVWQ+LPF 
Sbjct: 863  DLSNFYFDVAKDRLYVGGTTTFTRKSCQTVLAAHLLSIVKVVAPILPHLAEDVWQNLPFP 922

Query: 771  YAARDGDVAKFVFESRWPAVNDKWLAFPLKEVEFWGKILELRTEVNRTLEAARTGKLIGS 592
            +   DG VAKFVFES+WPA N+KWL+FP++E++FWGKILELRTEVN+ LEAAR GKLIGS
Sbjct: 923  HVLEDGSVAKFVFESKWPASNEKWLSFPIEEIDFWGKILELRTEVNKVLEAARMGKLIGS 982

Query: 591  SLEAKVYLYSFDDSLSERFKEMCETKNDADALYRIFITSQVEILSSLDNAQVENIPYTGE 412
            SLEAKVYLY+ D  L+ +F E+C   NDAD L+RIFITSQVE++  L+   +E IP+ GE
Sbjct: 983  SLEAKVYLYASDARLASKFHEICAASNDADPLHRIFITSQVEVIELLNEKLIETIPHAGE 1042

Query: 411  YVIEGDNKIWIGVSRAEGSKCERCWNFSPQVGSYAEHPSLCSRCFSAIDGQP 256
            ++IEG N++WIGVSRAEG KCERCWN++  VGS+ EHP+LC RC+  +  QP
Sbjct: 1043 FLIEGGNRVWIGVSRAEGMKCERCWNYTADVGSFVEHPTLCGRCYRIVAMQP 1094


>ref|NP_001190497.1| isoleucyl-tRNA synthetase [Arabidopsis thaliana]
            gi|332008381|gb|AED95764.1| isoleucyl-tRNA synthetase
            [Arabidopsis thaliana]
          Length = 1279

 Score = 1637 bits (4239), Expect = 0.0
 Identities = 786/1061 (74%), Positives = 900/1061 (84%), Gaps = 1/1061 (0%)
 Frame = -3

Query: 3435 QMSSYKAMSLRSFSSFRKAPSTTIFNFRRSSSAIFLAFLDITCYSTISNNEFHPSSKRRS 3256
            Q SSY+ +S +S S+ R+      F  +  SS    +FL ++ +ST  NNEF  SSKRRS
Sbjct: 208  QSSSYRVLSGKSCSNLRRNTPLDSFLAKGRSSVKAFSFLYVSRFSTEPNNEFGHSSKRRS 267

Query: 3255 RGPVMAAKKASKGEKLEDGKYKHTVDLPKTNFGMRANSLTREPEIQKLWEDNQVFKRLTD 3076
            RGPVMAAKKAS+GEK EDGKYK TVDLPKT FGMRANSLTREPE+QKLWE+NQVFKR++D
Sbjct: 268  RGPVMAAKKASEGEKQEDGKYKQTVDLPKTGFGMRANSLTREPELQKLWEENQVFKRVSD 327

Query: 3075 RNTGGNFVLHDGPPYANGDLHMGHALNKILKDMINRYKLLQNFKVHYVPGWDCHGLPIEL 2896
             N GG+F+LHDGPPYANGDLHMGHALNKILKD+INRYKLLQN+KV YVPGWDCHGLPIEL
Sbjct: 328  NNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWDCHGLPIEL 387

Query: 2895 KVLQSLDQEARKALTPXXXXXXXXXXXXXXXXAQMSSFKRYGVWADWDHPYLTLDPDYEA 2716
            KVLQSLDQE RK LTP                 QM SFKR+GVWADW++PYLTLDP+YEA
Sbjct: 388  KVLQSLDQEVRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVWADWNNPYLTLDPEYEA 447

Query: 2715 SQIEVFGQMVLQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHVSKSMYAAFRVLHAPTS 2536
            +QIEVFGQM L+GYI+RGRKPVHWSPSSRTALAEAELEYPEGH+SKS+YA F+++    +
Sbjct: 448  AQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIYAIFKLVGGAKT 507

Query: 2535 DGMLEFFPNLFLAIWTTTPWTMPANAAVAVNAKLQYAVVEVHRPLADLST-SSDGKKLVG 2359
              + EF PN++LA+WTTTPWTMPANAAVAVNAKLQY+VVEV     D ST +S+ KK+ G
Sbjct: 508  SLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSFSEDESTVTSNKKKIPG 567

Query: 2358 NLLKEQEKSFFIVAEDLVSTLEAKWGXXXXXXXXXLGSDLENCRYTHPIEKRECPVVIGG 2179
             +LK Q+K F IVA DLV  LEAKWG         LGSDLENCRYTHPI+ R+CPVVIGG
Sbjct: 568  KVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCRYTHPIDNRDCPVVIGG 627

Query: 2178 DYITTESGTGLVHTAPGHGQEDYATGLKYGLPVLSPVDDDGKFTEEAGEFSGLDVLGDGN 1999
            DYITTESGTGLVHTAPGHGQEDYATGLKYGLP++SPVDD+GKFTEEAG+F GL VLG+GN
Sbjct: 628  DYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFTEEAGQFRGLSVLGEGN 687

Query: 1998 AAVIKYLDKLSSLIMVEPYTHKYPYDWRTKKPTIFRATEQWFASVEGFRKAAMDAISHVT 1819
             AV+ YLD+  SL+M E Y HKYPYDWRTKKPTIFRATEQWFASVEGFR A MDAI++V 
Sbjct: 688  TAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAINNVK 747

Query: 1818 WTPSQAENRISAMASTRSDWCISRQRTWGVPIPVFYHVETKEPLMNEETISHIKSIISQK 1639
            W P QA NRISAM S+RSDWCISRQRTWGVPIP FYHV+TKEPLMNEETI+H+KSIISQK
Sbjct: 748  WVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQK 807

Query: 1638 GSDAWWYMSVEELLPDKYRGCASEYEKGTDTMDVWFDSGSSWAAVLDKREGLSYPADLYL 1459
            GSDAWWYMSVE+LLP+KYR  A++YEKGTDTMDVWFDSGSSWA VL KREGLS+PAD+YL
Sbjct: 808  GSDAWWYMSVEDLLPEKYRDKAADYEKGTDTMDVWFDSGSSWAGVLGKREGLSFPADVYL 867

Query: 1458 EGTDQHRGWFQSSLLTSVATKGKAPYSGVITHGFVLDERGLKMSKSLGNVVDPITVIEGG 1279
            EGTDQHRGWFQSSLLTS+AT+GKAPYS VITHGFVLDE+G+KMSKSLGNVVDP  VIEGG
Sbjct: 868  EGTDQHRGWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGG 927

Query: 1278 KNSGEAPSYGADVLRLWVSSVDYTGDVLIGPQVLRQMSDIYRKLRGTLRFLLANLHDWEA 1099
            KNS +AP+YGADV+RLWVSSVDYTGDVLIGPQ+LRQMSDIYRKLRGTLR+LL NLHDW  
Sbjct: 928  KNSKDAPAYGADVMRLWVSSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWRV 987

Query: 1098 GYAVPYSDLPVIDQHALFQLEHVVKNIGDSYENYQFFKIFQTIQRFVIVDLSNFYFDVAK 919
              AVPY DLP+IDQHALFQLE+VVKNI + YENYQFFKIFQ IQRF IVDLSNFYFD+AK
Sbjct: 988  DNAVPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIAK 1047

Query: 918  DRLYVGGTASFTRRSCQTVLAEHLLSITRVIAPILPHLAEDVWQHLPFQYAARDGDVAKF 739
            DRLY GGT+SFTRRSCQTVL+ HLLSI RVIAPI+PHLAEDVWQ+LPF+Y   DG  A+F
Sbjct: 1048 DRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGSAAEF 1107

Query: 738  VFESRWPAVNDKWLAFPLKEVEFWGKILELRTEVNRTLEAARTGKLIGSSLEAKVYLYSF 559
            VFE +WP +N++WL+FP ++V FW ++LELRTEVN+ LE AR  K+IGSSLEAKVYL++ 
Sbjct: 1108 VFELKWPTLNEQWLSFPAEDVLFWQRLLELRTEVNKVLELARNEKMIGSSLEAKVYLHTA 1167

Query: 558  DDSLSERFKEMCETKNDADALYRIFITSQVEILSSLDNAQVENIPYTGEYVIEGDNKIWI 379
            D  ++ +  EM E KN+AD L RIFITSQVE+LSS++   + ++ +TGEYV EG+NK+WI
Sbjct: 1168 DAGMAAKLLEMSEAKNEADTLQRIFITSQVEVLSSMEKEMISSVQHTGEYV-EGENKVWI 1226

Query: 378  GVSRAEGSKCERCWNFSPQVGSYAEHPSLCSRCFSAIDGQP 256
            GVSRAEGSKCERCWN+S QVGS+++HP+LC RCFS I   P
Sbjct: 1227 GVSRAEGSKCERCWNYSGQVGSFSDHPTLCGRCFSVIVANP 1267


>ref|NP_199714.2| isoleucyl-tRNA synthetase [Arabidopsis thaliana]
            gi|19424030|gb|AAL87306.1| putative isoleucyl-tRNA
            synthetase [Arabidopsis thaliana]
            gi|23296804|gb|AAN13174.1| putative isoleucyl-tRNA
            synthetase [Arabidopsis thaliana]
            gi|332008379|gb|AED95762.1| isoleucyl-tRNA synthetase
            [Arabidopsis thaliana]
          Length = 1093

 Score = 1637 bits (4239), Expect = 0.0
 Identities = 786/1061 (74%), Positives = 900/1061 (84%), Gaps = 1/1061 (0%)
 Frame = -3

Query: 3435 QMSSYKAMSLRSFSSFRKAPSTTIFNFRRSSSAIFLAFLDITCYSTISNNEFHPSSKRRS 3256
            Q SSY+ +S +S S+ R+      F  +  SS    +FL ++ +ST  NNEF  SSKRRS
Sbjct: 22   QSSSYRVLSGKSCSNLRRNTPLDSFLAKGRSSVKAFSFLYVSRFSTEPNNEFGHSSKRRS 81

Query: 3255 RGPVMAAKKASKGEKLEDGKYKHTVDLPKTNFGMRANSLTREPEIQKLWEDNQVFKRLTD 3076
            RGPVMAAKKAS+GEK EDGKYK TVDLPKT FGMRANSLTREPE+QKLWE+NQVFKR++D
Sbjct: 82   RGPVMAAKKASEGEKQEDGKYKQTVDLPKTGFGMRANSLTREPELQKLWEENQVFKRVSD 141

Query: 3075 RNTGGNFVLHDGPPYANGDLHMGHALNKILKDMINRYKLLQNFKVHYVPGWDCHGLPIEL 2896
             N GG+F+LHDGPPYANGDLHMGHALNKILKD+INRYKLLQN+KV YVPGWDCHGLPIEL
Sbjct: 142  NNNGGSFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVQYVPGWDCHGLPIEL 201

Query: 2895 KVLQSLDQEARKALTPXXXXXXXXXXXXXXXXAQMSSFKRYGVWADWDHPYLTLDPDYEA 2716
            KVLQSLDQE RK LTP                 QM SFKR+GVWADW++PYLTLDP+YEA
Sbjct: 202  KVLQSLDQEVRKELTPLKLRAKAAKFAKATVKTQMESFKRFGVWADWNNPYLTLDPEYEA 261

Query: 2715 SQIEVFGQMVLQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHVSKSMYAAFRVLHAPTS 2536
            +QIEVFGQM L+GYI+RGRKPVHWSPSSRTALAEAELEYPEGH+SKS+YA F+++    +
Sbjct: 262  AQIEVFGQMALKGYIYRGRKPVHWSPSSRTALAEAELEYPEGHISKSIYAIFKLVGGAKT 321

Query: 2535 DGMLEFFPNLFLAIWTTTPWTMPANAAVAVNAKLQYAVVEVHRPLADLST-SSDGKKLVG 2359
              + EF PN++LA+WTTTPWTMPANAAVAVNAKLQY+VVEV     D ST +S+ KK+ G
Sbjct: 322  SLLDEFIPNIYLAVWTTTPWTMPANAAVAVNAKLQYSVVEVQSFSEDESTVTSNKKKIPG 381

Query: 2358 NLLKEQEKSFFIVAEDLVSTLEAKWGXXXXXXXXXLGSDLENCRYTHPIEKRECPVVIGG 2179
             +LK Q+K F IVA DLV  LEAKWG         LGSDLENCRYTHPI+ R+CPVVIGG
Sbjct: 382  KVLKNQQKLFVIVATDLVPALEAKWGVKLSISKTFLGSDLENCRYTHPIDNRDCPVVIGG 441

Query: 2178 DYITTESGTGLVHTAPGHGQEDYATGLKYGLPVLSPVDDDGKFTEEAGEFSGLDVLGDGN 1999
            DYITTESGTGLVHTAPGHGQEDYATGLKYGLP++SPVDD+GKFTEEAG+F GL VLG+GN
Sbjct: 442  DYITTESGTGLVHTAPGHGQEDYATGLKYGLPLVSPVDDEGKFTEEAGQFRGLSVLGEGN 501

Query: 1998 AAVIKYLDKLSSLIMVEPYTHKYPYDWRTKKPTIFRATEQWFASVEGFRKAAMDAISHVT 1819
             AV+ YLD+  SL+M E Y HKYPYDWRTKKPTIFRATEQWFASVEGFR A MDAI++V 
Sbjct: 502  TAVVSYLDENMSLVMEESYAHKYPYDWRTKKPTIFRATEQWFASVEGFRTATMDAINNVK 561

Query: 1818 WTPSQAENRISAMASTRSDWCISRQRTWGVPIPVFYHVETKEPLMNEETISHIKSIISQK 1639
            W P QA NRISAM S+RSDWCISRQRTWGVPIP FYHV+TKEPLMNEETI+H+KSIISQK
Sbjct: 562  WVPHQAVNRISAMTSSRSDWCISRQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQK 621

Query: 1638 GSDAWWYMSVEELLPDKYRGCASEYEKGTDTMDVWFDSGSSWAAVLDKREGLSYPADLYL 1459
            GSDAWWYMSVE+LLP+KYR  A++YEKGTDTMDVWFDSGSSWA VL KREGLS+PAD+YL
Sbjct: 622  GSDAWWYMSVEDLLPEKYRDKAADYEKGTDTMDVWFDSGSSWAGVLGKREGLSFPADVYL 681

Query: 1458 EGTDQHRGWFQSSLLTSVATKGKAPYSGVITHGFVLDERGLKMSKSLGNVVDPITVIEGG 1279
            EGTDQHRGWFQSSLLTS+AT+GKAPYS VITHGFVLDE+G+KMSKSLGNVVDP  VIEGG
Sbjct: 682  EGTDQHRGWFQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGG 741

Query: 1278 KNSGEAPSYGADVLRLWVSSVDYTGDVLIGPQVLRQMSDIYRKLRGTLRFLLANLHDWEA 1099
            KNS +AP+YGADV+RLWVSSVDYTGDVLIGPQ+LRQMSDIYRKLRGTLR+LL NLHDW  
Sbjct: 742  KNSKDAPAYGADVMRLWVSSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLGNLHDWRV 801

Query: 1098 GYAVPYSDLPVIDQHALFQLEHVVKNIGDSYENYQFFKIFQTIQRFVIVDLSNFYFDVAK 919
              AVPY DLP+IDQHALFQLE+VVKNI + YENYQFFKIFQ IQRF IVDLSNFYFD+AK
Sbjct: 802  DNAVPYQDLPIIDQHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDLSNFYFDIAK 861

Query: 918  DRLYVGGTASFTRRSCQTVLAEHLLSITRVIAPILPHLAEDVWQHLPFQYAARDGDVAKF 739
            DRLY GGT+SFTRRSCQTVL+ HLLSI RVIAPI+PHLAEDVWQ+LPF+Y   DG  A+F
Sbjct: 862  DRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLPFEYRNEDGSAAEF 921

Query: 738  VFESRWPAVNDKWLAFPLKEVEFWGKILELRTEVNRTLEAARTGKLIGSSLEAKVYLYSF 559
            VFE +WP +N++WL+FP ++V FW ++LELRTEVN+ LE AR  K+IGSSLEAKVYL++ 
Sbjct: 922  VFELKWPTLNEQWLSFPAEDVLFWQRLLELRTEVNKVLELARNEKMIGSSLEAKVYLHTA 981

Query: 558  DDSLSERFKEMCETKNDADALYRIFITSQVEILSSLDNAQVENIPYTGEYVIEGDNKIWI 379
            D  ++ +  EM E KN+AD L RIFITSQVE+LSS++   + ++ +TGEYV EG+NK+WI
Sbjct: 982  DAGMAAKLLEMSEAKNEADTLQRIFITSQVEVLSSMEKEMISSVQHTGEYV-EGENKVWI 1040

Query: 378  GVSRAEGSKCERCWNFSPQVGSYAEHPSLCSRCFSAIDGQP 256
            GVSRAEGSKCERCWN+S QVGS+++HP+LC RCFS I   P
Sbjct: 1041 GVSRAEGSKCERCWNYSGQVGSFSDHPTLCGRCFSVIVANP 1081


>ref|XP_004134489.1| PREDICTED: isoleucine--tRNA ligase-like [Cucumis sativus]
          Length = 1093

 Score = 1625 bits (4207), Expect = 0.0
 Identities = 794/1070 (74%), Positives = 900/1070 (84%), Gaps = 3/1070 (0%)
 Frame = -3

Query: 3456 EFSKMASQMSSYKAMSLRSFSSFRKAPSTTIFNFRRSSSAIFLAFLDITCYSTISNNEFH 3277
            E +K   Q  SY  +S R  S+ R++  ++ F FR SSSA    FL +T YST  N+EF 
Sbjct: 18   EATKPFIQTPSYTVLSQRISSTLRRSYISS-FRFRGSSSAKVFTFLTVTRYSTYLNDEFP 76

Query: 3276 PSSKRRSRGPVMAAKKASKGEKLEDGKYKHTVDLPKTNFGMRANSLTREPEIQKLWEDNQ 3097
            PSSKRRSRGP+MAAKKASKG    DGKYKHTVDLPKT FGMRANSL REPEIQK+W+D+Q
Sbjct: 77   PSSKRRSRGPIMAAKKASKGTNEGDGKYKHTVDLPKTAFGMRANSLVREPEIQKIWDDHQ 136

Query: 3096 VFKRLTDRNTGGNFVLHDGPPYANGDLHMGHALNKILKDMINRYKLLQNFKVHYVPGWDC 2917
            VF R+ +RNTG NF+LHDGPPYANGDLHMGHALNKILKD+INRYKLLQN+KV +VPGWDC
Sbjct: 137  VFNRVVERNTGENFILHDGPPYANGDLHMGHALNKILKDIINRYKLLQNYKVRFVPGWDC 196

Query: 2916 HGLPIELKVLQSLDQEARKALTPXXXXXXXXXXXXXXXXAQMSSFKRYGVWADWDHPYLT 2737
            HGLPIELKVLQSLD+EARK LTP                AQM+SFKRYGVWADW++PYLT
Sbjct: 197  HGLPIELKVLQSLDKEARKDLTPIKLRDKAAKFAKATVKAQMASFKRYGVWADWNNPYLT 256

Query: 2736 LDPDYEASQIEVFGQMVLQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHVSKSMYAAFR 2557
            L P+YEA+QIEVFGQM +QGYIFRGRKPVHWSPSSRTALAEAELEYPEGH S+S+YA FR
Sbjct: 257  LSPEYEAAQIEVFGQMAIQGYIFRGRKPVHWSPSSRTALAEAELEYPEGHTSRSIYAIFR 316

Query: 2556 VL-HAPTSDGML-EFFPNLFLAIWTTTPWTMPANAAVAVNAKLQYAVVEVHRPLADLST- 2386
            ++   P+S G+L EF P+L LAIWTTTPWT+PANAAVAVNAKL YA++E+  P  D+S  
Sbjct: 317  LVKEGPSSGGVLQEFLPDLCLAIWTTTPWTIPANAAVAVNAKLHYAIIELLAPSEDVSVL 376

Query: 2385 SSDGKKLVGNLLKEQEKSFFIVAEDLVSTLEAKWGXXXXXXXXXLGSDLENCRYTHPIEK 2206
            + + KK  GN+LKE ++ F +VA DLV TLEAKWG         LGS+LENC Y HPI+K
Sbjct: 377  TGNKKKRPGNILKEGKRPFLVVASDLVPTLEAKWGVKLVVKKKLLGSELENCSYIHPIDK 436

Query: 2205 RECPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKYGLPVLSPVDDDGKFTEEAGEFS 2026
            +EC VVIGGDYITTE+GTGLVHTAPGHGQEDYATGLKYGLP++SPVDDDGKFTEEAG+FS
Sbjct: 437  KECQVVIGGDYITTETGTGLVHTAPGHGQEDYATGLKYGLPIISPVDDDGKFTEEAGQFS 496

Query: 2025 GLDVLGDGNAAVIKYLDKLSSLIMVEPYTHKYPYDWRTKKPTIFRATEQWFASVEGFRKA 1846
            GLDVLGDGN AV+KYLD   SLI+ E Y HKYPYDWRTKKPTIFRATEQWFASVEGFR+A
Sbjct: 497  GLDVLGDGNTAVVKYLDDHFSLILEESYEHKYPYDWRTKKPTIFRATEQWFASVEGFRQA 556

Query: 1845 AMDAISHVTWTPSQAENRISAMASTRSDWCISRQRTWGVPIPVFYHVETKEPLMNEETIS 1666
            A+DAI  V W P QAENRISAM S+RSDWCISRQRTWGVPIPVFYHVETKEPLMN++TI+
Sbjct: 557  AVDAIGEVKWIPPQAENRISAMTSSRSDWCISRQRTWGVPIPVFYHVETKEPLMNDDTIN 616

Query: 1665 HIKSIISQKGSDAWWYMSVEELLPDKYRGCASEYEKGTDTMDVWFDSGSSWAAVLDKREG 1486
            HIKSII++KGSDAWWYM+V+ LLPDKYR  AS+YEKGTDTMDVWFDSGSSWAAVL  R+G
Sbjct: 617  HIKSIIAKKGSDAWWYMAVDVLLPDKYREIASDYEKGTDTMDVWFDSGSSWAAVLGTRDG 676

Query: 1485 LSYPADLYLEGTDQHRGWFQSSLLTSVATKGKAPYSGVITHGFVLDERGLKMSKSLGNVV 1306
             S+PADLYLEGTDQHRGWFQSSLLT +ATKGKAPY+ V+THGFVLDE+GLKMSKSLGNVV
Sbjct: 677  FSFPADLYLEGTDQHRGWFQSSLLTCIATKGKAPYASVVTHGFVLDEKGLKMSKSLGNVV 736

Query: 1305 DPITVIEGGKNSGEAPSYGADVLRLWVSSVDYTGDVLIGPQVLRQMSDIYRKLRGTLRFL 1126
            DP  VIEGGKN  EAP YGADVLRLWVSSVDYTGDV+IG QVLRQMSDIYRKLRGTLRFL
Sbjct: 737  DPKGVIEGGKNQKEAPGYGADVLRLWVSSVDYTGDVMIGSQVLRQMSDIYRKLRGTLRFL 796

Query: 1125 LANLHDWEAGYAVPYSDLPVIDQHALFQLEHVVKNIGDSYENYQFFKIFQTIQRFVIVDL 946
            L NLHDW    AV Y DLP IDQHALFQLE+V+ NI +SYE+YQFFKIFQ IQRFVIVDL
Sbjct: 797  LGNLHDWTEENAVQYQDLPKIDQHALFQLENVINNIRESYESYQFFKIFQIIQRFVIVDL 856

Query: 945  SNFYFDVAKDRLYVGGTASFTRRSCQTVLAEHLLSITRVIAPILPHLAEDVWQHLPFQYA 766
            SNFYFDVAKDRLYVGG+ SFTRRSCQTVLA H++SI R+IAPILPHLAEDVWQ+LPFQ+ 
Sbjct: 857  SNFYFDVAKDRLYVGGSTSFTRRSCQTVLAAHVVSIARIIAPILPHLAEDVWQNLPFQHT 916

Query: 765  ARDGDVAKFVFESRWPAVNDKWLAFPLKEVEFWGKILELRTEVNRTLEAARTGKLIGSSL 586
              DG VAKFVFESRWP++N   L+ P +E++ W  ILELRTEVN+ LEAAR GKLIGSSL
Sbjct: 917  DDDGSVAKFVFESRWPSLNKTRLSLPEEEIDLWANILELRTEVNKVLEAARIGKLIGSSL 976

Query: 585  EAKVYLYSFDDSLSERFKEMCETKNDADALYRIFITSQVEILSSLDNAQVENIPYTGEYV 406
            EAKV+L++  D LS +  EMCE+ +DAD L RIFITSQVE+  S++   +EN+PYTGE +
Sbjct: 977  EAKVHLHAPGDGLSSKLCEMCESNHDADTLQRIFITSQVEVHQSIETEHIENVPYTGECL 1036

Query: 405  IEGDNKIWIGVSRAEGSKCERCWNFSPQVGSYAEHPSLCSRCFSAIDGQP 256
            I G NK+WIGVSRAEGSKCERCWNFS +VGS+ EHP+LC RCF+ + G P
Sbjct: 1037 I-GGNKVWIGVSRAEGSKCERCWNFSLRVGSFPEHPTLCKRCFNVVAGHP 1085