BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002860
         (1831 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002271630.1| PREDICTED: uncharacterized protein LOC100244...   491   e-136
ref|XP_002532724.1| inter-alpha-trypsin inhibitor heavy chain, p...   491   e-136
ref|XP_002331179.1| predicted protein [Populus trichocarpa] gi|2...   470   e-130
ref|XP_002304123.1| predicted protein [Populus trichocarpa] gi|2...   462   e-127
ref|XP_004141711.1| PREDICTED: uncharacterized protein LOC101206...   461   e-127

>ref|XP_002271630.1| PREDICTED: uncharacterized protein LOC100244700 [Vitis vinifera]
            gi|297742983|emb|CBI35850.3| unnamed protein product
            [Vitis vinifera]
          Length = 747

 Score =  491 bits (1265), Expect = e-136
 Identities = 248/428 (57%), Positives = 312/428 (72%)
 Frame = +1

Query: 547  GVEVSTTRSTFYTQLITTGDAYDMENMDSAKDGCLLKHQIYTLKVSQVDGGSNLNVKMRW 726
            GVEV  T  +  ++LIT  D  D       +DG  L+ +IYTLKV +V+GG+NL++K  W
Sbjct: 136  GVEVDVTGRSCNSRLITEEDTKDNGKPAKTEDGRFLRWRIYTLKVPKVEGGTNLSIKFSW 195

Query: 727  SQKMVYQDGQFCLCVPFSFPTYVIPVASNIIKREKILLNLKPGLRTQVLCESTSHPLKEL 906
            SQK++YQDGQF L +PFSFPT V P    I K+EKI LN+  G  T++LC++TSH LKEL
Sbjct: 196  SQKLLYQDGQFSLIIPFSFPTCVTPAGKKIPKKEKIRLNVNSGTGTEILCKATSHLLKEL 255

Query: 907  RRGVGELVFLYEAEVAXXXXXXXXXXXXVCSSNIFGSLLLQTPSLHDVDQREMFCFTLFP 1086
            R  VG+L FLYEAEV             V S+NIFG  L+Q+PS+HD DQREMFCF LFP
Sbjct: 256  RHQVGKLGFLYEAEVQTWSRADFNFSYTVSSNNIFGGALMQSPSVHDFDQREMFCFYLFP 315

Query: 1087 GSDNRRKAFRKVIIFVVDISASMRGGPIESAKTAVLEAISNLDPMDSFNIIAFNDSSVLF 1266
            GS+   K FRK ++F+VDIS SMRG P+E+ K AVL A+ NL+  DSFNIIAFN  + LF
Sbjct: 316  GSNISGKVFRKEVVFIVDISRSMRGRPLENTKDAVLAALLNLNTQDSFNIIAFNGDTCLF 375

Query: 1267 SLSMELASDETIEKAAEWISTCLIPKGGTNMMLPLNQAIEMVAETSDSVPSIFLITDGAV 1446
            S +M LA+ E +  A +WI+T    +GGTN++ PL+QAIEM+ +TS S+P IFLITDGAV
Sbjct: 376  SSTMVLATKEEVGNATQWINTNFNAEGGTNILNPLSQAIEMLTKTSGSMPLIFLITDGAV 435

Query: 1447 ENERVICNDVKKRLLDGGLNCPRICTFGIGSFCNHYFLNMLAQIGRGYHDAAYDTDSVIS 1626
            E+ER ICN V+  L +    CPRICTFGIGS+CNHYFL MLAQIGRGY+DAAYD DS+  
Sbjct: 436  EDERHICNVVEGHLRNRSPICPRICTFGIGSYCNHYFLQMLAQIGRGYYDAAYDADSIAF 495

Query: 1627 RMRRLFHSATSLILTNIKIDALEHMDKLELYPCRVPDLSTGCPVIVSGRYSGSFPDSIKA 1806
             M+RLF  A+S+IL NI I+ LEH+D LEL+P  +PDLS+G P+I+SGRY G FPDS+KA
Sbjct: 496  HMQRLFTKASSVILANITIETLEHLDSLELFPFHIPDLSSGSPLIISGRYHGEFPDSLKA 555

Query: 1807 SGILADLS 1830
            SG LAD+S
Sbjct: 556  SGSLADMS 563



 Score =  148 bits (373), Expect = 5e-33
 Identities = 71/108 (65%), Positives = 89/108 (82%), Gaps = 2/108 (1%)
 Frame = +1

Query: 43  MADQLADSVAYGLKLSKRVYYGKQASVSSAPKALTMDKSS--ETWLPKAPMVYAEILDPA 216
           MA+  + +V YGL+LSKR+YYGK  S +SAPK   M+KS   E++LP APMVYA I +P+
Sbjct: 1   MAEDFSTAVEYGLRLSKRIYYGKDRS-ASAPKPQEMEKSESQESFLPTAPMVYAVIPEPS 59

Query: 217 VVDNPDIPSYQPHVHGLCEPPALIPLHMHGIEMEVECYLDTAFVTVSG 360
           +VDNPD+PSYQP+VHG C+PPALIPLHM GI MEV+CYLDTAF++VSG
Sbjct: 60  IVDNPDVPSYQPYVHGRCDPPALIPLHMLGIAMEVDCYLDTAFISVSG 107


>ref|XP_002532724.1| inter-alpha-trypsin inhibitor heavy chain, putative [Ricinus
            communis] gi|223527532|gb|EEF29655.1| inter-alpha-trypsin
            inhibitor heavy chain, putative [Ricinus communis]
          Length = 752

 Score =  491 bits (1263), Expect = e-136
 Identities = 239/428 (55%), Positives = 313/428 (73%)
 Frame = +1

Query: 547  GVEVSTTRSTFYTQLITTGDAYDMENMDSAKDGCLLKHQIYTLKVSQVDGGSNLNVKMRW 726
            G+EV  T  ++++QLI   +  D        DG  LK  IYT K+ QV GG+ ++VK+ W
Sbjct: 134  GLEVDITGGSYHSQLIIAKETIDKGKTSKGGDGRYLKGSIYTFKIPQVVGGTTISVKVTW 193

Query: 727  SQKMVYQDGQFCLCVPFSFPTYVIPVASNIIKREKILLNLKPGLRTQVLCESTSHPLKEL 906
            SQK+ Y +GQFCL +PFSFP +V PVA  I KREKILLN+  G+  ++L   TSH LKEL
Sbjct: 194  SQKLTYNEGQFCLNIPFSFPAFVNPVAKKITKREKILLNVNSGVSKEILFRCTSHALKEL 253

Query: 907  RRGVGELVFLYEAEVAXXXXXXXXXXXXVCSSNIFGSLLLQTPSLHDVDQREMFCFTLFP 1086
            RR VG++ FLYE EV             V S ++FG + LQ+P L D+D+R+MFCF LFP
Sbjct: 254  RREVGKMAFLYEEEVTTWSIADLNFSYTVVSEDLFGEVFLQSPLLRDIDERQMFCFYLFP 313

Query: 1087 GSDNRRKAFRKVIIFVVDISASMRGGPIESAKTAVLEAISNLDPMDSFNIIAFNDSSVLF 1266
            G+   RKAFRK +IF++DIS SM+GGP+E+AK A++ ++S L+  DSFNIIAFND + LF
Sbjct: 314  GNKQSRKAFRKDVIFIIDISGSMKGGPLENAKNALMSSLSKLNSEDSFNIIAFNDETYLF 373

Query: 1267 SLSMELASDETIEKAAEWISTCLIPKGGTNMMLPLNQAIEMVAETSDSVPSIFLITDGAV 1446
            S  ME A+ E + KA+ W++  L   GGTN+M+PL QA++++A+T+DS+P IFLITDGAV
Sbjct: 374  SSLMEPATKEALSKASLWLNDNLTAGGGTNIMVPLKQAMKLLAQTTDSIPLIFLITDGAV 433

Query: 1447 ENERVICNDVKKRLLDGGLNCPRICTFGIGSFCNHYFLNMLAQIGRGYHDAAYDTDSVIS 1626
            ++ER ICN VK  L  GG   PRIC+FGIG++CNHYFL MLAQIGRGY D+AYD DSV  
Sbjct: 434  QDEREICNFVKGSLTSGGPISPRICSFGIGAYCNHYFLQMLAQIGRGYFDSAYDADSVDF 493

Query: 1627 RMRRLFHSATSLILTNIKIDALEHMDKLELYPCRVPDLSTGCPVIVSGRYSGSFPDSIKA 1806
            RM+RLF +A+S+IL N+ +DALEH+D LEL P R+PDLS G P++VSGRY+G FPDS+K 
Sbjct: 494  RMQRLFTTASSVILANVTVDALEHLDSLELLPFRIPDLSCGTPLVVSGRYNGKFPDSVKI 553

Query: 1807 SGILADLS 1830
            SGILAD+S
Sbjct: 554  SGILADMS 561



 Score =  122 bits (306), Expect = 3e-25
 Identities = 57/106 (53%), Positives = 78/106 (73%)
 Frame = +1

Query: 43  MADQLADSVAYGLKLSKRVYYGKQASVSSAPKALTMDKSSETWLPKAPMVYAEILDPAVV 222
           MA++    V YGL+LSKR+YYGK+   +  P+ ++   S E++LP A MVYA + +P  V
Sbjct: 1   MAEEFGTCVDYGLRLSKRIYYGKELPPAPEPE-MSKTVSWESYLPTAVMVYAVVPEPEAV 59

Query: 223 DNPDIPSYQPHVHGLCEPPALIPLHMHGIEMEVECYLDTAFVTVSG 360
           DNPD+PSYQP+VHG C+PPALIPL MHG+ M+V+C LD A V+ +G
Sbjct: 60  DNPDVPSYQPYVHGRCQPPALIPLQMHGVAMDVDCCLDYANVSFNG 105


>ref|XP_002331179.1| predicted protein [Populus trichocarpa] gi|222873300|gb|EEF10431.1|
            predicted protein [Populus trichocarpa]
          Length = 742

 Score =  470 bits (1210), Expect = e-130
 Identities = 235/427 (55%), Positives = 305/427 (71%)
 Frame = +1

Query: 547  GVEVSTTRSTFYTQLITTGDAYDMENMDSAKDGCLLKHQIYTLKVSQVDGGSNLNVKMRW 726
            GVEV  T  ++++QLI   DA   E +    +G L+K  +Y+ ++ +V GGS  ++K+ W
Sbjct: 141  GVEVDVTGRSYHSQLIQAEDANGNEKVSRGWNGRLIKGSMYSFEIPEVGGGSTFSIKVTW 200

Query: 727  SQKMVYQDGQFCLCVPFSFPTYVIPVASNIIKREKILLNLKPGLRTQVLCESTSHPLKEL 906
            SQK++Y +GQF L VPFSFP++V P+   I KREKILLN+  G+  ++LC   SH LKEL
Sbjct: 201  SQKLLYHEGQFSLNVPFSFPSFVNPIGKKISKREKILLNVNSGVGKEILCRCASHALKEL 260

Query: 907  RRGVGELVFLYEAEVAXXXXXXXXXXXXVCSSNIFGSLLLQTPSLHDVDQREMFCFTLFP 1086
            RR VG++ FLY+AEV             V S ++FG +LLQ+P L D D R+MFC  LFP
Sbjct: 261  RREVGKMGFLYDAEVLTWSSADFSFSYNVYSKDLFGGVLLQSPFLRDFDDRQMFCCYLFP 320

Query: 1087 GSDNRRKAFRKVIIFVVDISASMRGGPIESAKTAVLEAISNLDPMDSFNIIAFNDSSVLF 1266
            G +   KAFRK +IF++DIS SM+G P ESAK  +L ++  L+P DSFNIIAFN  + LF
Sbjct: 321  GDNQSMKAFRKEVIFLIDISGSMKGNPFESAKNGLLSSLQKLNPEDSFNIIAFNVETYLF 380

Query: 1267 SLSMELASDETIEKAAEWISTCLIPKGGTNMMLPLNQAIEMVAETSDSVPSIFLITDGAV 1446
            S  ME A+ E I KA +W++  L   GGTN++ PL QA++++AET+DS+P IFLITDGAV
Sbjct: 381  SSLMEQATKEAILKATQWLNDNLTADGGTNILAPLEQALKLLAETTDSIPLIFLITDGAV 440

Query: 1447 ENERVICNDVKKRLLDGGLNCPRICTFGIGSFCNHYFLNMLAQIGRGYHDAAYDTDSVIS 1626
            E+ER ICN VK  L  GG    RICTFGIG++CNHYFL MLAQIGRG+ D AYD DSV  
Sbjct: 441  EDERDICNFVKGSLTSGGSISLRICTFGIGTYCNHYFLRMLAQIGRGHFDTAYDADSVDF 500

Query: 1627 RMRRLFHSATSLILTNIKIDALEHMDKLELYPCRVPDLSTGCPVIVSGRYSGSFPDSIKA 1806
            RM+RLF +A+S+IL NI +DALE +D LEL P  +PDLS GCP+IVSGRYSG+FPDS+K 
Sbjct: 501  RMQRLFATASSIILANITVDALESLDSLELLPFCIPDLSCGCPLIVSGRYSGNFPDSVKL 560

Query: 1807 SGILADL 1827
            SGILAD+
Sbjct: 561  SGILADM 567



 Score =  117 bits (292), Expect = 1e-23
 Identities = 62/112 (55%), Positives = 77/112 (68%), Gaps = 6/112 (5%)
 Frame = +1

Query: 43  MADQLADSVAYGLKLSKRVYYGKQAS--VSSAPKALTMDKSSE---TWLPKAPMVYAEIL 207
           MA + A  V YGL LSKR+YYGK+ +  VS+A       KSSE   ++LP A M YA + 
Sbjct: 1   MAREFATCVEYGLSLSKRIYYGKEMTPAVSAAMTRSMSSKSSELAESYLPTAVMAYAVVP 60

Query: 208 DPAVVDNPDIPSYQPHVHGLCEPPALIPLHMHG-IEMEVECYLDTAFVTVSG 360
           +P +VDNPD+PSYQP+VHG CEPPALIPL MHG + ME++C  D A V  SG
Sbjct: 61  EPELVDNPDVPSYQPYVHGRCEPPALIPLQMHGAVAMEIDCCFDHANVCFSG 112


>ref|XP_002304123.1| predicted protein [Populus trichocarpa] gi|222841555|gb|EEE79102.1|
            predicted protein [Populus trichocarpa]
          Length = 757

 Score =  462 bits (1189), Expect = e-127
 Identities = 232/427 (54%), Positives = 307/427 (71%)
 Frame = +1

Query: 547  GVEVSTTRSTFYTQLITTGDAYDMENMDSAKDGCLLKHQIYTLKVSQVDGGSNLNVKMRW 726
            GVEV     + ++QLI   DA   E +    +G L++  +YT K+ QV+GG+ +++K+ W
Sbjct: 141  GVEVDVPGRSCHSQLIEAEDANYKEKVSKGGNGRLMRGSVYTFKIPQVEGGATVSIKVIW 200

Query: 727  SQKMVYQDGQFCLCVPFSFPTYVIPVASNIIKREKILLNLKPGLRTQVLCESTSHPLKEL 906
            SQK++Y +GQF L VPFSFP +V PV   I KREKILLN+  G+  ++LC+STSH LKEL
Sbjct: 201  SQKLLYHEGQFSLNVPFSFPAFVNPVGKKISKREKILLNVSSGVGKEILCKSTSHALKEL 260

Query: 907  RRGVGELVFLYEAEVAXXXXXXXXXXXXVCSSNIFGSLLLQTPSLHDVDQREMFCFTLFP 1086
            RR VG++ FLY+AEV             V S ++FG +LLQ+P L D+D R+MFCF LFP
Sbjct: 261  RREVGKMGFLYDAEVLTWSSADFSFSYNVYSKDLFGGVLLQSPLLRDIDDRQMFCFYLFP 320

Query: 1087 GSDNRRKAFRKVIIFVVDISASMRGGPIESAKTAVLEAISNLDPMDSFNIIAFNDSSVLF 1266
            G++   KAFRK +IF++DIS SM+GGP ESAK  +L ++  L+P DSFNIIAF   + LF
Sbjct: 321  GNNQSMKAFRKEVIFIIDISGSMKGGPFESAKNGLLSSLQKLNPEDSFNIIAFKMDTYLF 380

Query: 1267 SLSMELASDETIEKAAEWISTCLIPKGGTNMMLPLNQAIEMVAETSDSVPSIFLITDGAV 1446
            S  ME A++E I +A  W++  L   GGTN++ PL QAI+++AET++S+P IFLITDGAV
Sbjct: 381  SSVMEQATEEAIIEATRWLNDKLTADGGTNILGPLKQAIKLLAETTNSIPVIFLITDGAV 440

Query: 1447 ENERVICNDVKKRLLDGGLNCPRICTFGIGSFCNHYFLNMLAQIGRGYHDAAYDTDSVIS 1626
            E+ER ICN VK  L  GG    RI TFGIG++CNH+FL MLAQIGRG+ D AYD DSV  
Sbjct: 441  EDERDICNFVKGYLPSGGSISLRISTFGIGTYCNHHFLRMLAQIGRGHFDTAYDADSVDF 500

Query: 1627 RMRRLFHSATSLILTNIKIDALEHMDKLELYPCRVPDLSTGCPVIVSGRYSGSFPDSIKA 1806
            RM++LF +A+S+IL +I +DALEH+D LEL P  +PDLS G P+IVSGRYSG+FPD +K 
Sbjct: 501  RMQKLFTTASSIILADITVDALEHLDSLELLPFCIPDLSRGSPLIVSGRYSGNFPDPVKI 560

Query: 1807 SGILADL 1827
            SG LAD+
Sbjct: 561  SGTLADM 567



 Score =  115 bits (289), Expect = 3e-23
 Identities = 61/113 (53%), Positives = 79/113 (69%), Gaps = 7/113 (6%)
 Frame = +1

Query: 43  MADQLADSVAYGLKLSKRVYYGKQASVSSAPKALTM---DKSS---ETWLPKAPMVYAEI 204
           MA + A  V YGL LSKR+YYGK+ +  +A  A+T    +KSS   E++ P A M YA +
Sbjct: 1   MAREFATCVEYGLSLSKRIYYGKEMT-PAATAAMTRSVSEKSSDVAESYFPTAVMAYAVV 59

Query: 205 LDPAVVDNPDIPSYQPHVHGLCEPPALIPLHMHG-IEMEVECYLDTAFVTVSG 360
            +P +V+NPD+PSYQP+VHG CEPPALIPLHMHG + ME++C  D A V  SG
Sbjct: 60  SEPELVENPDVPSYQPYVHGRCEPPALIPLHMHGVVTMEIDCCFDHANVCFSG 112


>ref|XP_004141711.1| PREDICTED: uncharacterized protein LOC101206287 [Cucumis sativus]
            gi|449480498|ref|XP_004155911.1| PREDICTED:
            uncharacterized LOC101206287 [Cucumis sativus]
          Length = 754

 Score =  461 bits (1185), Expect = e-127
 Identities = 220/429 (51%), Positives = 308/429 (71%), Gaps = 1/429 (0%)
 Frame = +1

Query: 547  GVEVSTTRSTFYTQLITTGDAYDMENMDSAKDGCLLK-HQIYTLKVSQVDGGSNLNVKMR 723
            GVEV  T ++  T+L++  DA  +E +  ++DG  LK  +IYTLK+ +V+GG  L+V++ 
Sbjct: 133  GVEVDVTGTSHRTELVSMEDAEAIEKLTKSEDGKFLKGRRIYTLKIPKVEGGCTLSVRIN 192

Query: 724  WSQKMVYQDGQFCLCVPFSFPTYVIPVASNIIKREKILLNLKPGLRTQVLCESTSHPLKE 903
            WSQ++ Y D  FCL VPFSFP Y++P    I   +KILL++  G+ ++V+C+ TSHP+K 
Sbjct: 193  WSQRIPYLDDLFCLSVPFSFPAYLVPPGKKIKNSQKILLHINSGVSSEVVCKHTSHPMKI 252

Query: 904  LRRGVGELVFLYEAEVAXXXXXXXXXXXXVCSSNIFGSLLLQTPSLHDVDQREMFCFTLF 1083
            LRR VG L F  +AEV+            +  +++FG +LLQ+PSLHD DQREMFC  +F
Sbjct: 253  LRREVGNLSFSNDAEVSAWSNMDFDLSYSISPNDLFGGVLLQSPSLHDFDQREMFCLYIF 312

Query: 1084 PGSDNRRKAFRKVIIFVVDISASMRGGPIESAKTAVLEAISNLDPMDSFNIIAFNDSSVL 1263
            PG ++ R+ FRK ++F++DIS SM+ GP+ES K AVL ++S L+P D+FNII FN  + L
Sbjct: 313  PGQNHNRQVFRKEVVFIIDISGSMKDGPLESTKRAVLASLSQLNPEDAFNIIGFNGDTKL 372

Query: 1264 FSLSMELASDETIEKAAEWISTCLIPKGGTNMMLPLNQAIEMVAETSDSVPSIFLITDGA 1443
            FSL+ME A+ E I +A +WI+  L+  GGTN++LP+ QAI+M+AET +S+P IFLITDG+
Sbjct: 373  FSLTMEQATKEAITRATDWINANLVANGGTNILLPVEQAIKMLAETGNSIPLIFLITDGS 432

Query: 1444 VENERVICNDVKKRLLDGGLNCPRICTFGIGSFCNHYFLNMLAQIGRGYHDAAYDTDSVI 1623
            V+NER ICN VK  L  G    PR+CTFGIG+FCNHYFL ML++IGRG +DAAYD D + 
Sbjct: 433  VDNEREICNLVKASLKSGNKISPRLCTFGIGTFCNHYFLQMLSEIGRGIYDAAYDVDLID 492

Query: 1624 SRMRRLFHSATSLILTNIKIDALEHMDKLELYPCRVPDLSTGCPVIVSGRYSGSFPDSIK 1803
            +R + LF  A+SL L NI +DA +H+D  EL+P ++PDL+ G P+I+SGRY+G FP+S K
Sbjct: 493  TRFQTLFTKASSLFLANITVDAFKHLDSFELFPTQIPDLACGSPLIISGRYNGCFPESFK 552

Query: 1804 ASGILADLS 1830
             SG  AD+S
Sbjct: 553  VSGTSADMS 561



 Score =  136 bits (343), Expect = 2e-29
 Identities = 66/108 (61%), Positives = 81/108 (75%), Gaps = 2/108 (1%)
 Frame = +1

Query: 43  MADQLADSVAYGLKLSKRVYYGKQASVSSAPKALT--MDKSSETWLPKAPMVYAEILDPA 216
           MA + ++SV YGL LSKR+YYGK     S P AL   M + SE +LP+APMVYA I +P 
Sbjct: 1   MATEFSNSVEYGLHLSKRIYYGK----GSTPAALARQMSRVSEDYLPRAPMVYAVIPEPT 56

Query: 217 VVDNPDIPSYQPHVHGLCEPPALIPLHMHGIEMEVECYLDTAFVTVSG 360
           +VDNPDIPSYQP+VHG C PPALIPLHM+G+ ME+ C  DTAF+ V+G
Sbjct: 57  IVDNPDIPSYQPYVHGRCVPPALIPLHMNGVSMEINCCFDTAFICVNG 104