BLASTX nr result
ID: Bupleurum21_contig00002843
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00002843
(1856 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAF23892.1|AF152172_1 pectin methyl esterase [Solanum tuberosum] 779 0.0
emb|CAB95025.1| pectin methylesterase [Nicotiana tabacum] 770 0.0
gb|AAO85706.1| pectin methyl-esterase [Nicotiana benthamiana] 765 0.0
ref|XP_002304257.1| predicted protein [Populus trichocarpa] gi|2... 764 0.0
sp|P83948.1|PME3_CITSI RecName: Full=Pectinesterase 3; Short=PE ... 756 0.0
>gb|AAF23892.1|AF152172_1 pectin methyl esterase [Solanum tuberosum]
Length = 576
Score = 779 bits (2011), Expect = 0.0
Identities = 387/522 (74%), Positives = 438/522 (83%), Gaps = 1/522 (0%)
Frame = -1
Query: 1586 ATAAHSVLKSSCSSTLYPDLCYTTLASVPGMSKKITSLKDVIEQALNITHQVVGQTYSKI 1407
+++AH+++KS+CS+TL+P+LCY+ + +V SKK+TS KDVIE +LNIT + V + Y +
Sbjct: 55 SSSAHAIVKSACSNTLHPELCYSAIVNVTDFSKKVTSQKDVIELSLNITVKAVRRNYYAV 114
Query: 1406 KKMSK-HKHLTKREKGALKDCLELIDETLDEIHETVEDLEKYTSKISLKEHGDDLKTLIS 1230
K++ K K LT REK AL DCLE +DETLDE+H V DLE Y +K SLKEH +DLKTLIS
Sbjct: 115 KELIKTRKGLTPREKVALHDCLETMDETLDELHTAVADLELYPNKKSLKEHAEDLKTLIS 174
Query: 1229 SAITNQESCIDGFSHDGADKKVRDELLASEHHIEKMCSNALAMICNFTNTGSAXXXXXXX 1050
SAITNQE+C+DGFSHD ADKKVR LL + H+EKMCSNALAMICN TNT A
Sbjct: 175 SAITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMTNTDIANEMKLSG 234
Query: 1049 XXXXXXXXNVWPHWLSVGDRRLLQSSTVTPNVVVAADGSGNFKTXXXXXXXAPENSASRY 870
WP WLS GDRRLLQSSTVTP+VVVAADGSG++KT APE S+ RY
Sbjct: 235 SRKLVEDNGEWPEWLSAGDRRLLQSSTVTPDVVVAADGSGDYKTVSEAVAKAPEKSSKRY 294
Query: 869 VIRIKAGVYRESVEVPKKKINIMFLGDGRTNTIITASKNVQDGSTTFNSATVAAVGSGFL 690
VIRIKAGVYRE+V+VPKKK NIMF+GDGR+NTIITAS+NVQDGSTTF+SATVAAVG FL
Sbjct: 295 VIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITASRNVQDGSTTFHSATVAAVGEKFL 354
Query: 689 ARDITFQNTAGASKHQAVALRVGSDLSAFYKCDILAYQDSLYVHSNRQFFINCFIAGTVD 510
ARDITFQNTAGASKHQAVALRVGSDLSAFYKCDILAYQD+LYVHSNRQFF+ C +AGTVD
Sbjct: 355 ARDITFQNTAGASKHQAVALRVGSDLSAFYKCDILAYQDTLYVHSNRQFFVQCLVAGTVD 414
Query: 509 FIFGNAAVVLQDCDIHARRPDAGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQPVQS 330
FIFGN A VLQDCDIHARRP +GQKNMVTAQGRTDPNQNTGIVIQK RIGATSDL+PVQ
Sbjct: 415 FIFGNGAAVLQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQK 474
Query: 329 SFPTYLGRPWKQYSRTVVMQSSITNVINPAGWFPWDGNFALDTLYYGEYQNTGAGAATSG 150
SFPTYLGRPWK+YSRTV+MQSSIT+VI PAGW W+GNFAL+TL+YGEY NTGAGAATSG
Sbjct: 475 SFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALNTLFYGEYANTGAGAATSG 534
Query: 149 RVNWKGFKVITSSTEAQGFTPGNFIAGGSWLRSTTFPFSLGL 24
RV WKG KVITSSTEAQ +TPG+FIAGGSWL ST FPFSLGL
Sbjct: 535 RVKWKGHKVITSSTEAQAYTPGSFIAGGSWLSSTGFPFSLGL 576
>emb|CAB95025.1| pectin methylesterase [Nicotiana tabacum]
Length = 579
Score = 770 bits (1988), Expect = 0.0
Identities = 385/524 (73%), Positives = 436/524 (83%), Gaps = 4/524 (0%)
Frame = -1
Query: 1583 TAAHSVLKSSCSSTLYPDLCYTTLASVPGMSKKITSLKDVIEQALNITHQVVGQTYSKIK 1404
+AAH+++KS+C +TL+P+LCY+T+ASV SKK+TS KDVIE +LNIT + V + K++
Sbjct: 56 SAAHAIVKSACENTLHPELCYSTIASVSDFSKKVTSQKDVIELSLNITCRAVQHNFFKVE 115
Query: 1403 KMSK-HKHLTKREKGALKDCLELIDETLDEIHETVEDLEKYTSKISLKEHGDDLKTLISS 1227
K+ K K L REK AL DCLE IDETLDE+H ++DLE Y +K SLK H DDLKTLISS
Sbjct: 116 KLIKTRKGLKPREKVALHDCLETIDETLDELHTAIKDLELYPNKKSLKAHADDLKTLISS 175
Query: 1226 AITNQESCIDGFSHDGADKKVRDELLASEHHIEKMCSNALAMICNFTNTGSAXXXXXXXX 1047
AITNQE+C+DGFSHD ADKKVR LL + H+EKMCSNALAMICN T+T A
Sbjct: 176 AITNQETCLDGFSHDDADKKVRKALLKGQKHVEKMCSNALAMICNMTDTDIANEQKLKGT 235
Query: 1046 XXXXXXXN---VWPHWLSVGDRRLLQSSTVTPNVVVAADGSGNFKTXXXXXXXAPENSAS 876
WP WLS GDRRLLQSSTV P+VVVAADGSGNFKT APE S+
Sbjct: 236 TTNRKLREDNSEWPEWLSAGDRRLLQSSTVRPDVVVAADGSGNFKTVSEAVAKAPEKSSK 295
Query: 875 RYVIRIKAGVYRESVEVPKKKINIMFLGDGRTNTIITASKNVQDGSTTFNSATVAAVGSG 696
RYVIRIKAGVYRE+V+VPKKK NIMF+GDGR+NTIIT S+NV+DGSTTF+SATVAAVG
Sbjct: 296 RYVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVKDGSTTFHSATVAAVGEK 355
Query: 695 FLARDITFQNTAGASKHQAVALRVGSDLSAFYKCDILAYQDSLYVHSNRQFFINCFIAGT 516
FLARDITFQNTAGA+KHQAVALRVGSDLSAFY+CDILAYQDSLYVHSNRQ+F+ C IAGT
Sbjct: 356 FLARDITFQNTAGAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQYFVQCLIAGT 415
Query: 515 VDFIFGNAAVVLQDCDIHARRPDAGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQPV 336
VDFIFGNAA VLQ+CDIHARRP +GQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+PV
Sbjct: 416 VDFIFGNAAAVLQNCDIHARRPGSGQKNMVTAQGRSDPNQNTGIVIQKCRIGATSDLRPV 475
Query: 335 QSSFPTYLGRPWKQYSRTVVMQSSITNVINPAGWFPWDGNFALDTLYYGEYQNTGAGAAT 156
Q SFPTYLGRPWK+YSRTV+MQSSIT+VIN AGW W+GNFAL+TL+YGEYQNTGAGA T
Sbjct: 476 QKSFPTYLGRPWKEYSRTVIMQSSITDVINSAGWHEWNGNFALNTLFYGEYQNTGAGAGT 535
Query: 155 SGRVNWKGFKVITSSTEAQGFTPGNFIAGGSWLRSTTFPFSLGL 24
SGRV WKGFKVITS+TEAQ +TPG FIAGGSWL ST FPFSLGL
Sbjct: 536 SGRVKWKGFKVITSATEAQAYTPGRFIAGGSWLSSTGFPFSLGL 579
>gb|AAO85706.1| pectin methyl-esterase [Nicotiana benthamiana]
Length = 579
Score = 765 bits (1975), Expect = 0.0
Identities = 383/524 (73%), Positives = 433/524 (82%), Gaps = 4/524 (0%)
Frame = -1
Query: 1583 TAAHSVLKSSCSSTLYPDLCYTTLASVPGMSKKITSLKDVIEQALNITHQVVGQTYSKIK 1404
+AAH+++KS+C +TL+P+LCY+T+ASV SKK+TS KDVIE +LNIT + V + K++
Sbjct: 56 SAAHAIVKSACENTLHPELCYSTIASVSDFSKKVTSQKDVIELSLNITCRAVQHNFFKVE 115
Query: 1403 KMSK-HKHLTKREKGALKDCLELIDETLDEIHETVEDLEKYTSKISLKEHGDDLKTLISS 1227
K+ K K L REK AL DCLE IDETLDE+H ++DLE Y +K SLK H D LKTLISS
Sbjct: 116 KLIKTRKGLKPREKVALHDCLETIDETLDELHTAIKDLELYPNKKSLKAHADGLKTLISS 175
Query: 1226 AITNQESCIDGFSHDGADKKVRDELLASEHHIEKMCSNALAMICNFTNTGSAXXXXXXXX 1047
AITNQE+C+DGFSHD ADKKVR LL + H+EKMCSNALAMICN T+T A
Sbjct: 176 AITNQETCLDGFSHDDADKKVRKALLKGQKHVEKMCSNALAMICNMTDTDIANEQKLKGT 235
Query: 1046 XXXXXXXN---VWPHWLSVGDRRLLQSSTVTPNVVVAADGSGNFKTXXXXXXXAPENSAS 876
WP WL GDRRLLQSSTV P+VVVAADGSGNFKT APE S+
Sbjct: 236 TTNRKLREDNSEWPEWLPAGDRRLLQSSTVRPDVVVAADGSGNFKTVSEAVAKAPEKSSK 295
Query: 875 RYVIRIKAGVYRESVEVPKKKINIMFLGDGRTNTIITASKNVQDGSTTFNSATVAAVGSG 696
RYVIRIKAGVYRE+V+VPKKK NIMF+GDGR+NTIIT S+NV+DGSTTF+SATVAAVG
Sbjct: 296 RYVIRIKAGVYRENVDVPKKKTNIMFMGDGRSNTIITGSRNVKDGSTTFHSATVAAVGEK 355
Query: 695 FLARDITFQNTAGASKHQAVALRVGSDLSAFYKCDILAYQDSLYVHSNRQFFINCFIAGT 516
FLARDITFQNTAGA+KHQAVALRVGSDLSAFY+CDILAYQDSLYVHSNRQ+F+ C IAGT
Sbjct: 356 FLARDITFQNTAGAAKHQAVALRVGSDLSAFYRCDILAYQDSLYVHSNRQYFVQCLIAGT 415
Query: 515 VDFIFGNAAVVLQDCDIHARRPDAGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQPV 336
VDFIFGNAA VLQDCDIHARRP +GQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+PV
Sbjct: 416 VDFIFGNAAAVLQDCDIHARRPGSGQKNMVTAQGRSDPNQNTGIVIQKCRIGATSDLRPV 475
Query: 335 QSSFPTYLGRPWKQYSRTVVMQSSITNVINPAGWFPWDGNFALDTLYYGEYQNTGAGAAT 156
Q SFP YLGRPWK+YSRTV+MQSSIT+VIN AGW W+GNFAL+TL+YGEYQNTGAGA T
Sbjct: 476 QKSFPMYLGRPWKEYSRTVIMQSSITDVINSAGWHEWNGNFALNTLFYGEYQNTGAGAGT 535
Query: 155 SGRVNWKGFKVITSSTEAQGFTPGNFIAGGSWLRSTTFPFSLGL 24
SGRV WKGFKVITS+TEAQ +TPG FIAGGSWL ST FPFSLGL
Sbjct: 536 SGRVKWKGFKVITSATEAQAYTPGRFIAGGSWLSSTGFPFSLGL 579
>ref|XP_002304257.1| predicted protein [Populus trichocarpa] gi|222841689|gb|EEE79236.1|
predicted protein [Populus trichocarpa]
Length = 579
Score = 764 bits (1972), Expect = 0.0
Identities = 379/525 (72%), Positives = 432/525 (82%), Gaps = 3/525 (0%)
Frame = -1
Query: 1589 KATAAHSVLKSSCSSTLYPDLCYTTLASVPGMSKKITSLKDVIEQALNITHQVVGQTYSK 1410
K H++LKSSCSSTLYP+LCY+ +A+VPG + + S KDVIE ++N+T + V +
Sbjct: 55 KNEGTHAILKSSCSSTLYPELCYSAVATVPGATSNLASQKDVIELSINLTTKAVQHNFFT 114
Query: 1409 IKKMSKHKHLTKREKGALKDCLELIDETLDEIHETVEDLEKYTSKISLKEHGDDLKTLIS 1230
++K+ K LTKREK AL DCLE+IDETLDE+HE + DL Y + SLK+H DDLKTL+S
Sbjct: 115 VEKLIATKKLTKREKTALHDCLEIIDETLDELHEALVDLNDYPNNKSLKKHADDLKTLLS 174
Query: 1229 SAITNQESCIDGFSHDGADKKVRDELLASEHHIEKMCSNALAMICNFTNTGSAXXXXXXX 1050
SAITNQE+C+DGFSHD ADKKVR LL + H+EKMCSN LAMI N T+T A
Sbjct: 175 SAITNQETCLDGFSHDEADKKVRKALLKGQIHVEKMCSNVLAMIKNMTDTDVANELKTTN 234
Query: 1049 XXXXXXXXN---VWPHWLSVGDRRLLQSSTVTPNVVVAADGSGNFKTXXXXXXXAPENSA 879
WP W+SV DRRLLQSS+VTP+VVVAADGSGN+KT AP+ S+
Sbjct: 235 RKLMQEKEGNESEWPEWMSVADRRLLQSSSVTPDVVVAADGSGNYKTVSAAVAAAPKKSS 294
Query: 878 SRYVIRIKAGVYRESVEVPKKKINIMFLGDGRTNTIITASKNVQDGSTTFNSATVAAVGS 699
RY+IRIKAGVYRE+V+VPK K NIMF+GDGR TIITAS+NV DGSTTFNSATVAAVG
Sbjct: 295 KRYIIRIKAGVYRENVDVPKDKTNIMFMGDGRKTTIITASRNVVDGSTTFNSATVAAVGQ 354
Query: 698 GFLARDITFQNTAGASKHQAVALRVGSDLSAFYKCDILAYQDSLYVHSNRQFFINCFIAG 519
GFLAR ITFQNTAG SKHQAVALRVGSDLSAFY CD+LAYQD+LYVHSNRQFFINC +AG
Sbjct: 355 GFLARGITFQNTAGPSKHQAVALRVGSDLSAFYDCDMLAYQDTLYVHSNRQFFINCLVAG 414
Query: 518 TVDFIFGNAAVVLQDCDIHARRPDAGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQP 339
TVDFIFGNAAVVLQDCDIHARRP++GQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQP
Sbjct: 415 TVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQP 474
Query: 338 VQSSFPTYLGRPWKQYSRTVVMQSSITNVINPAGWFPWDGNFALDTLYYGEYQNTGAGAA 159
V+SSFPTYLGRPWK+YSRTV+MQSSIT+VI PAGWF W G+FAL+TLYY EYQN+GAGA
Sbjct: 475 VKSSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWFEWSGSFALNTLYYAEYQNSGAGAG 534
Query: 158 TSGRVNWKGFKVITSSTEAQGFTPGNFIAGGSWLRSTTFPFSLGL 24
TS RV WKG++VITS+TEAQ FTPGNFIAG SWLRSTTFPFSLGL
Sbjct: 535 TSRRVTWKGYRVITSATEAQRFTPGNFIAGSSWLRSTTFPFSLGL 579
>sp|P83948.1|PME3_CITSI RecName: Full=Pectinesterase 3; Short=PE 3; AltName: Full=Pectin
methylesterase 3; Flags: Precursor
Length = 584
Score = 756 bits (1951), Expect = 0.0
Identities = 376/519 (72%), Positives = 426/519 (82%), Gaps = 2/519 (0%)
Frame = -1
Query: 1574 HSVLKSSCSSTLYPDLCYTTLASVPGMSKKITSLKDVIEQALNITHQVVGQTYSKIKKMS 1395
H++LKSSCSST YPDLC++ +A+VP SKK+TS KDVIE +LNIT V Y I+K+
Sbjct: 66 HAILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLL 125
Query: 1394 KHKHLTKREKGALKDCLELIDETLDEIHETVEDLEKYTSKISLKEHGDDLKTLISSAITN 1215
K +LTKREK AL DCLE IDETLDE+H+ VEDLE+Y +K SL +H DDLKTL+S+A+TN
Sbjct: 126 KRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 185
Query: 1214 QESCIDGFSHDGADKKVRDELLASEHHIEKMCSNALAMICNFTNTGSAXXXXXXXXXXXX 1035
Q +C+DGFSHD A+K VRD L + H+EKMCSNALAMI N T+T
Sbjct: 186 QGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIE 245
Query: 1034 XXXNV--WPHWLSVGDRRLLQSSTVTPNVVVAADGSGNFKTXXXXXXXAPENSASRYVIR 861
V WP WLS GDRRLLQSS+VTPNVVVAADGSGNFKT AP+ RY+IR
Sbjct: 246 ETSTVDGWPAWLSTGDRRLLQSSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIR 305
Query: 860 IKAGVYRESVEVPKKKINIMFLGDGRTNTIITASKNVQDGSTTFNSATVAAVGSGFLARD 681
IKAGVYRE+VEV KK NIMF+GDGRT TIIT S+NV DGSTTF SATVA VG GFLARD
Sbjct: 306 IKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARD 365
Query: 680 ITFQNTAGASKHQAVALRVGSDLSAFYKCDILAYQDSLYVHSNRQFFINCFIAGTVDFIF 501
ITFQNTAG SKHQAVALRVG+DLSAFY CD+LAYQD+LYVHSNRQFF+NC IAGTVDFIF
Sbjct: 366 ITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIF 425
Query: 500 GNAAVVLQDCDIHARRPDAGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLQPVQSSFP 321
GNAA VLQ+CDIHAR+P++GQKNMVTAQGR DPNQNTGIVIQKSRIGATSDL+PVQ SFP
Sbjct: 426 GNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFP 485
Query: 320 TYLGRPWKQYSRTVVMQSSITNVINPAGWFPWDGNFALDTLYYGEYQNTGAGAATSGRVN 141
TYLGRPWK+YSRTV+MQSSIT+VI+PAGW WDGNFAL+TL+YGE+QN GAGA TSGRV
Sbjct: 486 TYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVK 545
Query: 140 WKGFKVITSSTEAQGFTPGNFIAGGSWLRSTTFPFSLGL 24
WKGF+VITS+TEAQ FTPG+FIAG SWL ST FPFSLGL
Sbjct: 546 WKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584