BLASTX nr result
ID: Bupleurum21_contig00002781
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00002781
(1728 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002276613.1| PREDICTED: cytochrome P450 710A1-like [Vitis... 751 0.0
gb|ABC59097.1| cytochrome P450 monooxygenase CYP710A15 [Medicago... 712 0.0
ref|XP_003593948.1| Cytochrome P450 monooxygenase CYP710A15 [Med... 712 0.0
gb|AEX63486.1| sterol 22-desaturase [Solanum lycopersicum] 707 0.0
ref|NP_001234514.1| sterol 22-desaturase [Solanum lycopersicum] ... 707 0.0
>ref|XP_002276613.1| PREDICTED: cytochrome P450 710A1-like [Vitis vinifera]
Length = 509
Score = 751 bits (1940), Expect = 0.0
Identities = 361/496 (72%), Positives = 413/496 (83%), Gaps = 1/496 (0%)
Frame = +1
Query: 13 SSSTPYILSLLAFFIFIEQFTYLKKKNTLPGPSIVFPFLGNAISLVRNPTLFWDTQSSFA 192
+S PY+LSL+A +F+EQ +YLK+K +PGP VFPF+GNA+SL+RNPT FWD QSS A
Sbjct: 12 ASVAPYLLSLIALLVFLEQISYLKRKRLIPGPPFVFPFIGNAVSLIRNPTKFWDIQSSLA 71
Query: 193 KSTPAGVSANYVIGKFMVFIRSTDLSHKIFANVRPDAFHLVGHPFGKKLFGEHNLIYMMG 372
+S+ G+SANY++GKF+VFIRSTDLSHKIFANVRPDAFHLVGHPFGKKLFGEHNLIY MG
Sbjct: 72 RSSDLGISANYIVGKFIVFIRSTDLSHKIFANVRPDAFHLVGHPFGKKLFGEHNLIYTMG 131
Query: 373 QDHKDLRRRIAPNFTPRALSTYTHTQQKIIIKHLACWLDVA-KGNSSSIPIRILCRDMNL 549
Q HKD+RRRIAPNFTPRAL+ YT QQ II+KHL W +A K + + I +R+LCR+MNL
Sbjct: 132 QQHKDIRRRIAPNFTPRALAAYTSLQQVIILKHLMAWEALASKASPTPISLRLLCREMNL 191
Query: 550 ETSQKVFVGPYLTAEAQKRFNYDYNFFNVGLMKLPIDLPGFAFRNARLAVSRLIETLGGC 729
+TSQ VFVGPYL+ EA++RFN DYN FNVGLMKLP DLPGFAFR ARLAV RLI+TL GC
Sbjct: 192 DTSQTVFVGPYLSQEARERFNRDYNLFNVGLMKLPFDLPGFAFREARLAVDRLIKTLAGC 251
Query: 730 AKLSKEKMKGGDEPTCLIDFWMQETLRXXXXXXXXXXXXXXHSSDIEIGGHIFDFLFAAQ 909
SK M+ G+EP CLIDFWMQETLR HS + EIGGH+FDFLFAAQ
Sbjct: 252 TDDSKATMEAGEEPRCLIDFWMQETLREIAAATDSGEPLPPHSGNAEIGGHLFDFLFAAQ 311
Query: 910 DASTSSLLWAVTLLDSHPAVLDRVRQEVAAIWTPESDTYITAEQLREMKYTEAVAREVIR 1089
DASTSSLLWAVTLLDSHP VL +VR+EVA IW+PESDT ITAEQLREMKYTEAVAREV+R
Sbjct: 312 DASTSSLLWAVTLLDSHPEVLAKVREEVAGIWSPESDTLITAEQLREMKYTEAVAREVVR 371
Query: 1090 LKAPATLVPHIAGEDFPLTDEYTIPKGAIVFPSVYESSFQGXXXXXXXXXXXXMPDRQED 1269
++APAT+VPHIAGEDF LT+ YTIPKG IVFPSV+ESSFQG M RQED
Sbjct: 372 IRAPATMVPHIAGEDFQLTESYTIPKGTIVFPSVFESSFQGFPDPERFEPERFMEHRQED 431
Query: 1270 QLYKRNFLAFGAGAHQCVGQRYAINHLVLFIAMFTSLIDFKRDRTDGCDDINYVPTICPK 1449
+LYK+NFLAFGAGAHQCVGQRYAINHLVLFIAMFTSL+DFKR RTDGCDDI YVPTICPK
Sbjct: 432 RLYKKNFLAFGAGAHQCVGQRYAINHLVLFIAMFTSLVDFKRHRTDGCDDIAYVPTICPK 491
Query: 1450 DDCRVYLSKRCAKFPN 1497
DDC+VYLS+RCA++P+
Sbjct: 492 DDCKVYLSRRCARYPS 507
>gb|ABC59097.1| cytochrome P450 monooxygenase CYP710A15 [Medicago truncatula]
Length = 515
Score = 712 bits (1839), Expect = 0.0
Identities = 346/493 (70%), Positives = 403/493 (81%), Gaps = 1/493 (0%)
Frame = +1
Query: 25 PYILSLLAFFIFIEQFTYLKKKNTLPGPSIVFPFLGNAISLVRNPTLFWDTQSSFAKSTP 204
PY++ + F + IEQ +YL KK ++PGPS+V PF+GNAISLVR+PT FW+ QS+ +KS
Sbjct: 17 PYLICFILFLLLIEQISYLNKKRSIPGPSLVLPFIGNAISLVRDPTKFWEVQSTLSKS-- 74
Query: 205 AGVSANYVIGKFMVFIRSTDLSHKIFANVRPDAFHLVGHPFGKKLFGEHNLIYMMGQDHK 384
AG SANY+IG F+VFIR T+LSHK+F+NV+P+AFHLVGHPFGKKLFGEHNLIYMMGQ+HK
Sbjct: 75 AGFSANYIIGLFIVFIRDTELSHKVFSNVKPNAFHLVGHPFGKKLFGEHNLIYMMGQEHK 134
Query: 385 DLRRRIAPNFTPRALSTYTHTQQKIIIKHLACWLDVAKGNS-SSIPIRILCRDMNLETSQ 561
+LRRRIAPNFTP+ALSTYT QQ II+KHL WL + + S PIR+L RDMNLETSQ
Sbjct: 135 NLRRRIAPNFTPKALSTYTELQQIIILKHLKSWLHKSHAQAHESFPIRVLTRDMNLETSQ 194
Query: 562 KVFVGPYLTAEAQKRFNYDYNFFNVGLMKLPIDLPGFAFRNARLAVSRLIETLGGCAKLS 741
VFVGPYL +A++RF +DY FNVGLMKLP D PG AFRNARLAV RL TLGGC S
Sbjct: 195 TVFVGPYLGLKARERFEHDYFLFNVGLMKLPFDFPGTAFRNARLAVDRLAGTLGGCTAAS 254
Query: 742 KEKMKGGDEPTCLIDFWMQETLRXXXXXXXXXXXXXXHSSDIEIGGHIFDFLFAAQDAST 921
KEKM+ G+EP+CLIDFWMQ+TLR S++ EIGG++FDFLFAAQDAST
Sbjct: 255 KEKMEKGEEPSCLIDFWMQDTLREIEESKLNGVTVPPFSTNAEIGGYLFDFLFAAQDAST 314
Query: 922 SSLLWAVTLLDSHPAVLDRVRQEVAAIWTPESDTYITAEQLREMKYTEAVAREVIRLKAP 1101
SSLLWAVTLLDSHP VL RVR+EVA IW+PES+T IT+EQLREMKYT+AVAREVIR + P
Sbjct: 315 SSLLWAVTLLDSHPEVLARVREEVAGIWSPESNTLITSEQLREMKYTQAVAREVIRYRPP 374
Query: 1102 ATLVPHIAGEDFPLTDEYTIPKGAIVFPSVYESSFQGXXXXXXXXXXXXMPDRQEDQLYK 1281
ATLVPH+A E FPLT+ YTIPKGAIVFPS +ESSFQG +RQEDQ++K
Sbjct: 375 ATLVPHVAAEPFPLTESYTIPKGAIVFPSAFESSFQGFTEPDRFDPDRFSEERQEDQIFK 434
Query: 1282 RNFLAFGAGAHQCVGQRYAINHLVLFIAMFTSLIDFKRDRTDGCDDINYVPTICPKDDCR 1461
RNFLAFGAG HQCVGQRYA+NHLVLFIAMFTSLIDFKRDRTDGCD+I YVPTICPKDDCR
Sbjct: 435 RNFLAFGAGPHQCVGQRYALNHLVLFIAMFTSLIDFKRDRTDGCDEIVYVPTICPKDDCR 494
Query: 1462 VYLSKRCAKFPNL 1500
V+LSKRC ++P+L
Sbjct: 495 VFLSKRCTRYPSL 507
>ref|XP_003593948.1| Cytochrome P450 monooxygenase CYP710A15 [Medicago truncatula]
gi|355482996|gb|AES64199.1| Cytochrome P450 monooxygenase
CYP710A15 [Medicago truncatula]
Length = 566
Score = 712 bits (1839), Expect = 0.0
Identities = 346/493 (70%), Positives = 403/493 (81%), Gaps = 1/493 (0%)
Frame = +1
Query: 25 PYILSLLAFFIFIEQFTYLKKKNTLPGPSIVFPFLGNAISLVRNPTLFWDTQSSFAKSTP 204
PY++ + F + IEQ +YL KK ++PGPS+V PF+GNAISLVR+PT FW+ QS+ +KS
Sbjct: 17 PYLICFILFLLLIEQISYLNKKRSIPGPSLVLPFIGNAISLVRDPTKFWEVQSTLSKS-- 74
Query: 205 AGVSANYVIGKFMVFIRSTDLSHKIFANVRPDAFHLVGHPFGKKLFGEHNLIYMMGQDHK 384
AG SANY+IG F+VFIR T+LSHK+F+NV+P+AFHLVGHPFGKKLFGEHNLIYMMGQ+HK
Sbjct: 75 AGFSANYIIGLFIVFIRDTELSHKVFSNVKPNAFHLVGHPFGKKLFGEHNLIYMMGQEHK 134
Query: 385 DLRRRIAPNFTPRALSTYTHTQQKIIIKHLACWLDVAKGNS-SSIPIRILCRDMNLETSQ 561
+LRRRIAPNFTP+ALSTYT QQ II+KHL WL + + S PIR+L RDMNLETSQ
Sbjct: 135 NLRRRIAPNFTPKALSTYTELQQIIILKHLKSWLHKSHAQAHESFPIRVLTRDMNLETSQ 194
Query: 562 KVFVGPYLTAEAQKRFNYDYNFFNVGLMKLPIDLPGFAFRNARLAVSRLIETLGGCAKLS 741
VFVGPYL +A++RF +DY FNVGLMKLP D PG AFRNARLAV RL TLGGC S
Sbjct: 195 TVFVGPYLGLKARERFEHDYFLFNVGLMKLPFDFPGTAFRNARLAVDRLAGTLGGCTAAS 254
Query: 742 KEKMKGGDEPTCLIDFWMQETLRXXXXXXXXXXXXXXHSSDIEIGGHIFDFLFAAQDAST 921
KEKM+ G+EP+CLIDFWMQ+TLR S++ EIGG++FDFLFAAQDAST
Sbjct: 255 KEKMEKGEEPSCLIDFWMQDTLREIEESKLNGVTVPPFSTNAEIGGYLFDFLFAAQDAST 314
Query: 922 SSLLWAVTLLDSHPAVLDRVRQEVAAIWTPESDTYITAEQLREMKYTEAVAREVIRLKAP 1101
SSLLWAVTLLDSHP VL RVR+EVA IW+PES+T IT+EQLREMKYT+AVAREVIR + P
Sbjct: 315 SSLLWAVTLLDSHPEVLARVREEVAGIWSPESNTLITSEQLREMKYTQAVAREVIRYRPP 374
Query: 1102 ATLVPHIAGEDFPLTDEYTIPKGAIVFPSVYESSFQGXXXXXXXXXXXXMPDRQEDQLYK 1281
ATLVPH+A E FPLT+ YTIPKGAIVFPS +ESSFQG +RQEDQ++K
Sbjct: 375 ATLVPHVAAEPFPLTESYTIPKGAIVFPSAFESSFQGFTEPDRFDPDRFSEERQEDQIFK 434
Query: 1282 RNFLAFGAGAHQCVGQRYAINHLVLFIAMFTSLIDFKRDRTDGCDDINYVPTICPKDDCR 1461
RNFLAFGAG HQCVGQRYA+NHLVLFIAMFTSLIDFKRDRTDGCD+I YVPTICPKDDCR
Sbjct: 435 RNFLAFGAGPHQCVGQRYALNHLVLFIAMFTSLIDFKRDRTDGCDEIVYVPTICPKDDCR 494
Query: 1462 VYLSKRCAKFPNL 1500
V+LSKRC ++P+L
Sbjct: 495 VFLSKRCTRYPSL 507
>gb|AEX63486.1| sterol 22-desaturase [Solanum lycopersicum]
Length = 501
Score = 707 bits (1824), Expect = 0.0
Identities = 332/497 (66%), Positives = 401/497 (80%), Gaps = 1/497 (0%)
Frame = +1
Query: 1 WEIISSSTPYILSLLAFFIFIEQFTYLKKKNTLPGPSIVFPFLGNAISLVRNPTLFWDTQ 180
W ++S PY +S +AF + +EQ +Y+KKK LPGP++VFPFLGN I LV NPT FWD Q
Sbjct: 5 WGLLSPWIPYFISFIAFLLLLEQISYIKKKRFLPGPTLVFPFLGNVIPLVTNPTKFWDLQ 64
Query: 181 SSFAKSTPAGVSANYVIGKFMVFIRSTDLSHKIFANVRPDAFHLVGHPFGKKLFGEHNLI 360
S+ AKST G S NY+IGKF+++I STDLSHK+FANVRPDAFHL+GHPFGKKLFGEHNLI
Sbjct: 65 SALAKSTSHGFSVNYIIGKFILYIHSTDLSHKVFANVRPDAFHLIGHPFGKKLFGEHNLI 124
Query: 361 YMMGQDHKDLRRRIAPNFTPRALSTYTHTQQKIIIKHLACWLDVA-KGNSSSIPIRILCR 537
YM GQ+HKDLRRRIAPNFTP+AL TYT QQ+IIIKH WLD A K ++ IP+R+LCR
Sbjct: 125 YMFGQEHKDLRRRIAPNFTPKALGTYTDIQQRIIIKHFKSWLDEASKSPNTPIPLRLLCR 184
Query: 538 DMNLETSQKVFVGPYLTAEAQKRFNYDYNFFNVGLMKLPIDLPGFAFRNARLAVSRLIET 717
DMNL+TSQ VFVGPYL E++KRFN DYN+FNVGLMKLP+DLPGFAFRNARLAV RL++T
Sbjct: 185 DMNLDTSQAVFVGPYLDGESRKRFNVDYNYFNVGLMKLPVDLPGFAFRNARLAVGRLVDT 244
Query: 718 LGGCAKLSKEKMKGGDEPTCLIDFWMQETLRXXXXXXXXXXXXXXHSSDIEIGGHIFDFL 897
L C + S KMK +EPTCLIDFWMQE LR S+ E+GG++FDFL
Sbjct: 245 LSVCVEQSLNKMKNEEEPTCLIDFWMQENLREINEAKINGLQKPFQYSNKELGGYLFDFL 304
Query: 898 FAAQDASTSSLLWAVTLLDSHPAVLDRVRQEVAAIWTPESDTYITAEQLREMKYTEAVAR 1077
FAAQDASTS+LLWA+ LLDSHP VL++VR +VA W+PES+ +TAE LREMKY EAVAR
Sbjct: 305 FAAQDASTSALLWAIVLLDSHPQVLEKVRSDVARFWSPESEEPLTAEMLREMKYLEAVAR 364
Query: 1078 EVIRLKAPATLVPHIAGEDFPLTDEYTIPKGAIVFPSVYESSFQGXXXXXXXXXXXXMPD 1257
E+IR++APAT+VPHIAGE+F LT++Y IPKG IVFPSV++SSFQG M +
Sbjct: 365 EIIRIRAPATMVPHIAGEEFRLTEDYVIPKGTIVFPSVFDSSFQGFPEPEKFEPDRFMEE 424
Query: 1258 RQEDQLYKRNFLAFGAGAHQCVGQRYAINHLVLFIAMFTSLIDFKRDRTDGCDDINYVPT 1437
RQE+++YK+NFLAFGAG H CVGQ+YAINHL+LFIAMFT+LIDFKR +TDGCDDI+Y+PT
Sbjct: 425 RQEERVYKKNFLAFGAGPHACVGQKYAINHLMLFIAMFTALIDFKRHKTDGCDDISYIPT 484
Query: 1438 ICPKDDCRVYLSKRCAK 1488
I PKDDC+V+L+ RC +
Sbjct: 485 IAPKDDCKVFLAHRCTR 501
>ref|NP_001234514.1| sterol 22-desaturase [Solanum lycopersicum]
gi|161701428|gb|ABX75534.1| sterol 22-desaturase [Solanum
lycopersicum] gi|377657500|gb|AFB74183.1| sterol
22-desaturase [Solanum lycopersicum]
gi|377657502|gb|AFB74184.1| sterol 22-desaturase [Solanum
lycopersicum] gi|377657504|gb|AFB74185.1| sterol
22-desaturase [Solanum lycopersicum]
gi|377657506|gb|AFB74186.1| sterol 22-desaturase [Solanum
lycopersicum] gi|377657508|gb|AFB74187.1| sterol
22-desaturase [Solanum lycopersicum]
gi|377657510|gb|AFB74188.1| sterol 22-desaturase [Solanum
lycopersicum] gi|377657512|gb|AFB74189.1| sterol
22-desaturase [Solanum lycopersicum]
gi|377657514|gb|AFB74190.1| sterol 22-desaturase [Solanum
lycopersicum]
Length = 501
Score = 707 bits (1824), Expect = 0.0
Identities = 332/497 (66%), Positives = 401/497 (80%), Gaps = 1/497 (0%)
Frame = +1
Query: 1 WEIISSSTPYILSLLAFFIFIEQFTYLKKKNTLPGPSIVFPFLGNAISLVRNPTLFWDTQ 180
W ++S PY +S +AF + +EQ +Y+KKK LPGP++VFPFLGN I LV NPT FWD Q
Sbjct: 5 WGLLSPWIPYFISFIAFLLLLEQISYIKKKRFLPGPTLVFPFLGNVIPLVTNPTKFWDLQ 64
Query: 181 SSFAKSTPAGVSANYVIGKFMVFIRSTDLSHKIFANVRPDAFHLVGHPFGKKLFGEHNLI 360
S+ AKST G S NY+IGKF+++I STDLSHK+FANVRPDAFHL+GHPFGKKLFGEHNLI
Sbjct: 65 SALAKSTSHGFSVNYIIGKFILYIHSTDLSHKVFANVRPDAFHLIGHPFGKKLFGEHNLI 124
Query: 361 YMMGQDHKDLRRRIAPNFTPRALSTYTHTQQKIIIKHLACWLDVA-KGNSSSIPIRILCR 537
YM GQ+HKDLRRRIAPNFTP+AL TYT QQ+IIIKH WLD A K ++ IP+R+LCR
Sbjct: 125 YMFGQEHKDLRRRIAPNFTPKALGTYTDIQQRIIIKHFKSWLDEASKSPNTPIPLRLLCR 184
Query: 538 DMNLETSQKVFVGPYLTAEAQKRFNYDYNFFNVGLMKLPIDLPGFAFRNARLAVSRLIET 717
DMNL+TSQ VFVGPYL E++KRFN DYN+FNVGLMKLP+DLPGFAFRNARLAV RL++T
Sbjct: 185 DMNLDTSQTVFVGPYLDGESRKRFNVDYNYFNVGLMKLPVDLPGFAFRNARLAVGRLVDT 244
Query: 718 LGGCAKLSKEKMKGGDEPTCLIDFWMQETLRXXXXXXXXXXXXXXHSSDIEIGGHIFDFL 897
L C + S KMK +EPTCLIDFWMQE LR S+ E+GG++FDFL
Sbjct: 245 LSVCVEQSLNKMKNEEEPTCLIDFWMQENLREINEAKINGLQKPFQYSNKELGGYLFDFL 304
Query: 898 FAAQDASTSSLLWAVTLLDSHPAVLDRVRQEVAAIWTPESDTYITAEQLREMKYTEAVAR 1077
FAAQDASTS+LLWA+ LLDSHP VL++VR +VA W+PES+ +TAE LREMKY EAVAR
Sbjct: 305 FAAQDASTSALLWAIVLLDSHPQVLEKVRSDVARFWSPESEEPLTAEMLREMKYLEAVAR 364
Query: 1078 EVIRLKAPATLVPHIAGEDFPLTDEYTIPKGAIVFPSVYESSFQGXXXXXXXXXXXXMPD 1257
E+IR++APAT+VPHIAGE+F LT++Y IPKG IVFPSV++SSFQG M +
Sbjct: 365 EIIRIRAPATMVPHIAGEEFRLTEDYVIPKGTIVFPSVFDSSFQGFPEPEKFEPDRFMEE 424
Query: 1258 RQEDQLYKRNFLAFGAGAHQCVGQRYAINHLVLFIAMFTSLIDFKRDRTDGCDDINYVPT 1437
RQE+++YK+NFLAFGAG H CVGQ+YAINHL+LFIAMFT+LIDFKR +TDGCDDI+Y+PT
Sbjct: 425 RQEERVYKKNFLAFGAGPHACVGQKYAINHLMLFIAMFTALIDFKRHKTDGCDDISYIPT 484
Query: 1438 ICPKDDCRVYLSKRCAK 1488
I PKDDC+V+L+ RC +
Sbjct: 485 IAPKDDCKVFLAHRCTR 501