BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002758
         (1512 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAB62808.1| S-adenosyl-methionine-sterol-C- methyltransferase...   416   e-113
ref|XP_003527489.1| PREDICTED: 24-methylenesterol C-methyltransf...   414   e-113
ref|XP_002510600.1| s-adenosyl-l-methionine:delta24-sterol-C-met...   413   e-113
gb|ABY49843.1| 24-sterol C-methyltransferase [Gossypium hirsutum]     412   e-112
ref|NP_001241131.1| 24-methylenesterol C-methyltransferase 2-lik...   412   e-112

>gb|AAB62808.1| S-adenosyl-methionine-sterol-C- methyltransferase [Nicotiana tabacum]
          Length = 357

 Score =  416 bits (1068), Expect = e-113
 Identities = 198/227 (87%), Positives = 212/227 (93%), Gaps = 1/227 (0%)
 Frame = -2

Query: 1478 MDSLPLLCTAAVIA-GGLYWFICILGSAEQKGKRAVDLSGGSIDNDKVQDNYDNYWSFFK 1302
            MDSL L+CTAA++A GGLYWF+CILGSAE KGKRAV LSGGSI+ + VQDNY  YWSFF+
Sbjct: 1    MDSLTLICTAALLAAGGLYWFVCILGSAEVKGKRAVQLSGGSIEKENVQDNYKQYWSFFR 60

Query: 1301 RPKEIETADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSNLEATQIHEEMAVDLL 1122
            RPKEIETADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKS+ EAT+IHEEMAVDL+
Sbjct: 61   RPKEIETADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHREATRIHEEMAVDLI 120

Query: 1121 NVKPGDKILDAGCGVGGPMRAIAAHSGANVVGITINEYQVNRARMHNKKAGLDSLCEVVC 942
             VKPG +ILDAGCGVGGPMRAIAAHS ANVVGITINEYQV RARMHNKKAGLDSLCEVVC
Sbjct: 121  GVKPGARILDAGCGVGGPMRAIAAHSRANVVGITINEYQVKRARMHNKKAGLDSLCEVVC 180

Query: 941  GNFLEMPFPDNTFDGVYSIEATCHAPKLEEVYAEIYRVLKPGSLYVS 801
            GNFL+MPFPDN+FDG YSIEATCHAPKLEEVY EIYRV+KPGSLYVS
Sbjct: 181  GNFLQMPFPDNSFDGAYSIEATCHAPKLEEVYKEIYRVMKPGSLYVS 227



 Score = 98.6 bits (244), Expect(2) = 1e-37
 Identities = 43/51 (84%), Positives = 48/51 (94%)
 Frame = -3

Query: 652 YSDISEIAKKVGFEVVKEKDLAKPPARPWWTRLKMGRIAYWRNHILVMILS 500
           Y DI+E+AKKVGFEVVKEKDLA+PP+ PWWTRLKMGRIAYWRNHILV IL+
Sbjct: 262 YKDIAEVAKKVGFEVVKEKDLARPPSNPWWTRLKMGRIAYWRNHILVTILA 312



 Score = 85.9 bits (211), Expect(2) = 1e-37
 Identities = 39/44 (88%), Positives = 42/44 (95%)
 Frame = -1

Query: 423 FLGIAPKGTVDVHEMLFVTADYLTRGGETKIFTPMHMILCRKPE 292
           FLGIAPKGTVDVHEMLFVTADYL++GGE  IF+PMHMILCRKPE
Sbjct: 313 FLGIAPKGTVDVHEMLFVTADYLSKGGEKGIFSPMHMILCRKPE 356


>ref|XP_003527489.1| PREDICTED: 24-methylenesterol C-methyltransferase 2-like [Glycine
            max]
          Length = 363

 Score =  414 bits (1064), Expect = e-113
 Identities = 194/226 (85%), Positives = 209/226 (92%)
 Frame = -2

Query: 1478 MDSLPLLCTAAVIAGGLYWFICILGSAEQKGKRAVDLSGGSIDNDKVQDNYDNYWSFFKR 1299
            MDSL L CT A++AGGLYWF+C+LG AEQKGKRA DLSGGSI  +KVQDNY  YWSFF+R
Sbjct: 1    MDSLSLFCTGALLAGGLYWFVCVLGPAEQKGKRATDLSGGSISAEKVQDNYKQYWSFFRR 60

Query: 1298 PKEIETADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSNLEATQIHEEMAVDLLN 1119
            PKEIETADKVP FVDTFYNLVTDIYEWGWGQSFHFSPSIPGKS+ EAT++HEEMAVDL+ 
Sbjct: 61   PKEIETADKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHREATRLHEEMAVDLIE 120

Query: 1118 VKPGDKILDAGCGVGGPMRAIAAHSGANVVGITINEYQVNRARMHNKKAGLDSLCEVVCG 939
             KPG+KILD GCGVGGPMRAIAAHS ANVVGITINEYQVNRARMHNKKAGLDSLCEVVCG
Sbjct: 121  AKPGNKILDVGCGVGGPMRAIAAHSRANVVGITINEYQVNRARMHNKKAGLDSLCEVVCG 180

Query: 938  NFLEMPFPDNTFDGVYSIEATCHAPKLEEVYAEIYRVLKPGSLYVS 801
            NFL+MPF DN+FDG YSIEATCHAPKLEEVYAEI+RVLKPG+LYVS
Sbjct: 181  NFLKMPFVDNSFDGAYSIEATCHAPKLEEVYAEIFRVLKPGALYVS 226



 Score = 89.7 bits (221), Expect(2) = 1e-33
 Identities = 38/51 (74%), Positives = 47/51 (92%)
 Frame = -3

Query: 652 YSDISEIAKKVGFEVVKEKDLAKPPARPWWTRLKMGRIAYWRNHILVMILS 500
           Y++I+E A+KVGF VVKE+DLAKPPA PWW+RLKMGRIAYWRNHI+V +L+
Sbjct: 261 YAEIAETARKVGFAVVKERDLAKPPALPWWSRLKMGRIAYWRNHIVVTVLA 311



 Score = 81.6 bits (200), Expect(2) = 1e-33
 Identities = 37/42 (88%), Positives = 40/42 (95%)
 Frame = -1

Query: 420 LGIAPKGTVDVHEMLFVTADYLTRGGETKIFTPMHMILCRKP 295
           LGIAPKGTVDVHEMLF TADYLTRGG++ IF+PMHMILCRKP
Sbjct: 313 LGIAPKGTVDVHEMLFKTADYLTRGGDSGIFSPMHMILCRKP 354


>ref|XP_002510600.1| s-adenosyl-l-methionine:delta24-sterol-C-methyltransferase, putative
            [Ricinus communis] gi|223551301|gb|EEF52787.1|
            s-adenosyl-l-methionine:delta24-sterol-C-
            methyltransferase, putative [Ricinus communis]
          Length = 361

 Score =  413 bits (1061), Expect = e-113
 Identities = 193/226 (85%), Positives = 210/226 (92%)
 Frame = -2

Query: 1478 MDSLPLLCTAAVIAGGLYWFICILGSAEQKGKRAVDLSGGSIDNDKVQDNYDNYWSFFKR 1299
            MDS  L CT A++A GLYWF+CILG AE KGKRAVDLSGGSI  +KVQDNY  YWSFF+R
Sbjct: 1    MDSFSLFCTGALLAVGLYWFVCILGPAEVKGKRAVDLSGGSISAEKVQDNYKQYWSFFRR 60

Query: 1298 PKEIETADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSNLEATQIHEEMAVDLLN 1119
            PKEIETA+KVP FVDTFYNLVTDIYEWGWGQSFHFSPS+PGKS+ +AT++HEEMAVDL++
Sbjct: 61   PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSVPGKSHRDATRLHEEMAVDLID 120

Query: 1118 VKPGDKILDAGCGVGGPMRAIAAHSGANVVGITINEYQVNRARMHNKKAGLDSLCEVVCG 939
            VKPGD+ILD GCGVGGPMRAIAAHS ANVVGITINEYQVNRAR+HNKKAGLDSLCEVVCG
Sbjct: 121  VKPGDRILDVGCGVGGPMRAIAAHSRANVVGITINEYQVNRARLHNKKAGLDSLCEVVCG 180

Query: 938  NFLEMPFPDNTFDGVYSIEATCHAPKLEEVYAEIYRVLKPGSLYVS 801
            NFLEMPFPDN+FDG YSIEATCHAPKLEEVYAEI+RVLKPGSLYVS
Sbjct: 181  NFLEMPFPDNSFDGAYSIEATCHAPKLEEVYAEIFRVLKPGSLYVS 226



 Score = 97.8 bits (242), Expect(2) = 1e-37
 Identities = 44/51 (86%), Positives = 48/51 (94%)
 Frame = -3

Query: 652 YSDISEIAKKVGFEVVKEKDLAKPPARPWWTRLKMGRIAYWRNHILVMILS 500
           Y DI+E A+KVGFEVVKEKDLAKPPA+PWWTRLKMGRIAYWRNHILV +LS
Sbjct: 261 YIDIAETARKVGFEVVKEKDLAKPPAQPWWTRLKMGRIAYWRNHILVTMLS 311



 Score = 87.0 bits (214), Expect(2) = 1e-37
 Identities = 40/43 (93%), Positives = 41/43 (95%)
 Frame = -1

Query: 420 LGIAPKGTVDVHEMLFVTADYLTRGGETKIFTPMHMILCRKPE 292
           +GIAPKGTVDVHEMLF TADYLTRGGET IFTPMHMILCRKPE
Sbjct: 313 IGIAPKGTVDVHEMLFKTADYLTRGGETGIFTPMHMILCRKPE 355


>gb|ABY49843.1| 24-sterol C-methyltransferase [Gossypium hirsutum]
          Length = 361

 Score =  412 bits (1059), Expect = e-112
 Identities = 191/226 (84%), Positives = 211/226 (93%)
 Frame = -2

Query: 1478 MDSLPLLCTAAVIAGGLYWFICILGSAEQKGKRAVDLSGGSIDNDKVQDNYDNYWSFFKR 1299
            MDSL L CT A++AGGLYWF+C+LG AEQKGKRAVDLSGGSI  +KV+DNY  YWSFF+R
Sbjct: 1    MDSLSLFCTGALLAGGLYWFVCVLGPAEQKGKRAVDLSGGSISAEKVRDNYKQYWSFFRR 60

Query: 1298 PKEIETADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSNLEATQIHEEMAVDLLN 1119
            PKEIETA+KVP FVDTFYNLVTDIYEWGWGQSFHFSPSIPGKS+ +AT++HEEMAVDL++
Sbjct: 61   PKEIETAEKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLID 120

Query: 1118 VKPGDKILDAGCGVGGPMRAIAAHSGANVVGITINEYQVNRARMHNKKAGLDSLCEVVCG 939
            VKPGD+ILD GCGVGGPMRAIAAHS A VVGITIN+YQVNRARMHNKKAGLDSLCEVVCG
Sbjct: 121  VKPGDRILDVGCGVGGPMRAIAAHSQAKVVGITINDYQVNRARMHNKKAGLDSLCEVVCG 180

Query: 938  NFLEMPFPDNTFDGVYSIEATCHAPKLEEVYAEIYRVLKPGSLYVS 801
            NFLEMPF DN+FDG YSIEATCHAPKLE+VYAE++RVLKPGSLYVS
Sbjct: 181  NFLEMPFQDNSFDGAYSIEATCHAPKLEDVYAEVFRVLKPGSLYVS 226



 Score = 99.4 bits (246), Expect(2) = 6e-35
 Identities = 44/51 (86%), Positives = 49/51 (96%)
 Frame = -3

Query: 652 YSDISEIAKKVGFEVVKEKDLAKPPARPWWTRLKMGRIAYWRNHILVMILS 500
           YSDI+E AKKVGFEVVKEKDLAKPP+ PWWTRLKMGRIAYWRNHILV++L+
Sbjct: 261 YSDIAEAAKKVGFEVVKEKDLAKPPSLPWWTRLKMGRIAYWRNHILVIVLA 311



 Score = 76.3 bits (186), Expect(2) = 6e-35
 Identities = 34/43 (79%), Positives = 40/43 (93%)
 Frame = -1

Query: 420 LGIAPKGTVDVHEMLFVTADYLTRGGETKIFTPMHMILCRKPE 292
           +GIAPKGTVDVH+MLF TADYLTRGG++ IF+PMHMIL RKP+
Sbjct: 313 IGIAPKGTVDVHDMLFKTADYLTRGGDSGIFSPMHMILLRKPK 355


>ref|NP_001241131.1| 24-methylenesterol C-methyltransferase 2-like [Glycine max]
            gi|242755433|gb|ACS93763.1| sterol 24-C methyltransferase
            2-1 [Glycine max]
          Length = 361

 Score =  412 bits (1058), Expect = e-112
 Identities = 191/226 (84%), Positives = 209/226 (92%)
 Frame = -2

Query: 1478 MDSLPLLCTAAVIAGGLYWFICILGSAEQKGKRAVDLSGGSIDNDKVQDNYDNYWSFFKR 1299
            MD L L CT A++AGGLYWF+C+LG AEQKGKRA DLSGGSI  +KVQDNY  YWSFF+R
Sbjct: 1    MDPLSLFCTGALLAGGLYWFVCVLGPAEQKGKRATDLSGGSISAEKVQDNYKQYWSFFRR 60

Query: 1298 PKEIETADKVPAFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSNLEATQIHEEMAVDLLN 1119
            PKEIETADKVP FVDTFYNLVTDIYEWGWGQSFHFSPSIPGKS+ +AT++HEEMAVDL+ 
Sbjct: 61   PKEIETADKVPDFVDTFYNLVTDIYEWGWGQSFHFSPSIPGKSHRDATRLHEEMAVDLIE 120

Query: 1118 VKPGDKILDAGCGVGGPMRAIAAHSGANVVGITINEYQVNRARMHNKKAGLDSLCEVVCG 939
             KPG++ILD GCGVGGPMRAIAAHS ANVVGITINEYQVNRARMHNKKAGL+SLCEVVCG
Sbjct: 121  AKPGNRILDVGCGVGGPMRAIAAHSRANVVGITINEYQVNRARMHNKKAGLESLCEVVCG 180

Query: 938  NFLEMPFPDNTFDGVYSIEATCHAPKLEEVYAEIYRVLKPGSLYVS 801
            NFL+MPFPDN+FDG YSIEATCHAPKLEEVYAEI+RVLKPG+LYVS
Sbjct: 181  NFLKMPFPDNSFDGAYSIEATCHAPKLEEVYAEIFRVLKPGALYVS 226



 Score = 92.4 bits (228), Expect(2) = 2e-34
 Identities = 39/51 (76%), Positives = 48/51 (94%)
 Frame = -3

Query: 652 YSDISEIAKKVGFEVVKEKDLAKPPARPWWTRLKMGRIAYWRNHILVMILS 500
           Y+DI+E A+KVGF VVKE+DLAKPPA+PWW+RLKMGRIAYWRNHI+V +L+
Sbjct: 261 YTDIAETARKVGFAVVKERDLAKPPAQPWWSRLKMGRIAYWRNHIVVTVLA 311



 Score = 81.6 bits (200), Expect(2) = 2e-34
 Identities = 37/42 (88%), Positives = 40/42 (95%)
 Frame = -1

Query: 420 LGIAPKGTVDVHEMLFVTADYLTRGGETKIFTPMHMILCRKP 295
           LGIAPKGTVDVHEMLF TADYLTRGG++ IF+PMHMILCRKP
Sbjct: 313 LGIAPKGTVDVHEMLFKTADYLTRGGDSGIFSPMHMILCRKP 354