BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002753
         (1664 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P14912.1|4CL1_PETCR RecName: Full=4-coumarate--CoA ligase 1; ...   774   0.0  
sp|P14913.1|4CL2_PETCR RecName: Full=4-coumarate--CoA ligase 1; ...   770   0.0  
gb|ACL31667.1| 4-coumarate coenzyme A ligase [Paulownia fortunei]     723   0.0  
sp|O24540.1|4CL_VANPL RecName: Full=4-coumarate--CoA ligase; Sho...   719   0.0  
gb|AEO52694.1| 4-coumarate:CoA ligase [Petunia x hybrida]             718   0.0  

>sp|P14912.1|4CL1_PETCR RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName:
            Full=4-coumaroyl-CoA synthase 1 gi|20432|emb|CAA31696.1|
            unnamed protein product [Petroselinum crispum]
          Length = 544

 Score =  774 bits (1999), Expect(2) = 0.0
 Identities = 387/425 (91%), Positives = 406/425 (95%)
 Frame = +1

Query: 358  QVKASNTKLIITQACYVPKVQDYAAEHDIQIVCIDAPPENCLSFSELSGADESEMPEVNI 537
            Q+KAS  KLIITQACYV KV+DYAAE +IQI+CID  P++CL FS+L  ADESEMPEV I
Sbjct: 120  QLKASQAKLIITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVI 179

Query: 538  NSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMICVLPLFHI 717
            NSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY+HSEDVMIC+LPLFHI
Sbjct: 180  NSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHI 239

Query: 718  YSLNAVLCCGLRAGVAILIMQKFEIVAFLELIQKYKVTIGPFVPPIVLAIAKSPEVDNYD 897
            YSLNAVLCCGLRAGV ILIMQKF+IV FLELIQKYKVTIGPFVPPIVLAIAKSP VD YD
Sbjct: 240  YSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYD 299

Query: 898  LSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSG 1077
            LSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSG
Sbjct: 300  LSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSG 359

Query: 1078 ACGTVVRNAEMKIVDPETNTSLPRNQPGEICIRGDQIMKGYLNDPESTERTIDKEGWLHT 1257
            ACGTVVRNAEMKIVDPETN SLPRNQ GEICIRGDQIMKGYLNDPEST  TID+EGWLHT
Sbjct: 360  ACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHT 419

Query: 1258 GDIGYIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVSMIDEKAGEV 1437
            GDIG+IDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVV MIDEKAGEV
Sbjct: 420  GDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEV 479

Query: 1438 PVAFVVRSNGFTTTEDEIKQFVSKQVIYYKRIYRVFFVDAIPKSPSGKILRKDLRARLAS 1617
            PVAFVVR+NGFTTTE+EIKQFVSKQV++YKRI+RVFFVDAIPKSPSGKILRKDLRAR+AS
Sbjct: 480  PVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIAS 539

Query: 1618 GDLPK 1632
            GDLPK
Sbjct: 540  GDLPK 544



 Score =  176 bits (447), Expect(2) = 0.0
 Identities = 83/94 (88%), Positives = 90/94 (95%)
 Frame = +3

Query: 69  VTPKQDIIFRSKLPDIYIPKHLPLHSYCFENISKVSSRPCLINGATGETFTYAQVELISR 248
           V PK+D+IFRSKLPDIYIPKHLPLH+YCFENISKV  + CLINGATGETFTY+QVEL+SR
Sbjct: 5   VAPKEDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSR 64

Query: 249 KVASGLNKLGIKQGDTIMLLLPNSPEYFFAFLGA 350
           KVASGLNKLGI+QGDTIMLLLPNSPEYFFAFLGA
Sbjct: 65  KVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGA 98


>sp|P14913.1|4CL2_PETCR RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName:
            Full=4-coumaroyl-CoA synthase 1 gi|20436|emb|CAA31697.1|
            unnamed protein product [Petroselinum crispum]
          Length = 544

 Score =  770 bits (1988), Expect(2) = 0.0
 Identities = 385/425 (90%), Positives = 406/425 (95%)
 Frame = +1

Query: 358  QVKASNTKLIITQACYVPKVQDYAAEHDIQIVCIDAPPENCLSFSELSGADESEMPEVNI 537
            Q+KAS  KLIITQACYV KV+DYAAE +IQI+CID  P++CL FS+L  ADESEMPEV I
Sbjct: 120  QLKASLAKLIITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVVI 179

Query: 538  NSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMICVLPLFHI 717
            +SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY+HSEDVMIC+LPLFHI
Sbjct: 180  DSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHI 239

Query: 718  YSLNAVLCCGLRAGVAILIMQKFEIVAFLELIQKYKVTIGPFVPPIVLAIAKSPEVDNYD 897
            YSLNAVLCCGLRAGV ILIMQKF+IV FLELIQKYKVTIGPFVPPIVLAIAKSP VD YD
Sbjct: 240  YSLNAVLCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYD 299

Query: 898  LSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSG 1077
            LSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSG
Sbjct: 300  LSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSG 359

Query: 1078 ACGTVVRNAEMKIVDPETNTSLPRNQPGEICIRGDQIMKGYLNDPESTERTIDKEGWLHT 1257
            ACGTVVRNAEMKIVDPETN SLPRNQ GEICIRGDQIMKGYLNDPEST  TID+EGWLHT
Sbjct: 360  ACGTVVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHT 419

Query: 1258 GDIGYIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVSMIDEKAGEV 1437
            GDIG+IDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVV MIDEKAGEV
Sbjct: 420  GDIGFIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEV 479

Query: 1438 PVAFVVRSNGFTTTEDEIKQFVSKQVIYYKRIYRVFFVDAIPKSPSGKILRKDLRARLAS 1617
            PVAFVVR+NGFTTTE+EIKQFVSKQV++YKRI+RVFFVDAIPKSPSGKILRKDLRA++AS
Sbjct: 480  PVAFVVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIAS 539

Query: 1618 GDLPK 1632
            GDLPK
Sbjct: 540  GDLPK 544



 Score =  176 bits (447), Expect(2) = 0.0
 Identities = 83/94 (88%), Positives = 90/94 (95%)
 Frame = +3

Query: 69  VTPKQDIIFRSKLPDIYIPKHLPLHSYCFENISKVSSRPCLINGATGETFTYAQVELISR 248
           V PK+D+IFRSKLPDIYIPKHLPLH+YCFENISKV  + CLINGATGETFTY+QVEL+SR
Sbjct: 5   VAPKEDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSR 64

Query: 249 KVASGLNKLGIKQGDTIMLLLPNSPEYFFAFLGA 350
           KVASGLNKLGI+QGDTIMLLLPNSPEYFFAFLGA
Sbjct: 65  KVASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGA 98


>gb|ACL31667.1| 4-coumarate coenzyme A ligase [Paulownia fortunei]
          Length = 543

 Score =  723 bits (1867), Expect(2) = 0.0
 Identities = 364/421 (86%), Positives = 385/421 (91%)
 Frame = +1

Query: 358  QVKASNTKLIITQACYVPKVQDYAAEHDIQIVCIDAPPENCLSFSELSGADESEMPEVNI 537
            Q KASN KLIITQACYV KV+DYA E  ++++CIDAP  +CL FSEL+ ADE +MP V I
Sbjct: 120  QAKASNAKLIITQACYVEKVRDYALEKGVKVMCIDAPSADCLQFSELTSADERDMPAVKI 179

Query: 538  NSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMICVLPLFHI 717
            + DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVM+CVLPLFHI
Sbjct: 180  HPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHI 239

Query: 718  YSLNAVLCCGLRAGVAILIMQKFEIVAFLELIQKYKVTIGPFVPPIVLAIAKSPEVDNYD 897
            YSLN+VL CGLR G AILIMQKF+IV FLELIQKYKVTIGPFVPPIVLAI KSP VD YD
Sbjct: 240  YSLNSVLLCGLRVGAAILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIVKSPVVDKYD 299

Query: 898  LSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSG 1077
            LSSVRTVMSGAAPLGKELEDAVR KFPNAKLGQGYGMTEAGPVLAMCLAFAKEP+EIKSG
Sbjct: 300  LSSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSG 359

Query: 1078 ACGTVVRNAEMKIVDPETNTSLPRNQPGEICIRGDQIMKGYLNDPESTERTIDKEGWLHT 1257
            ACGTVVRNAEMKIVD ET  SL RNQPGEICIRGDQIMKGYLND ESTE TIDK+GWLHT
Sbjct: 360  ACGTVVRNAEMKIVDIETGASLGRNQPGEICIRGDQIMKGYLNDLESTEGTIDKDGWLHT 419

Query: 1258 GDIGYIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVSMIDEKAGEV 1437
            GDIG+ID DDELFIVDRLKEIIKYKGFQVAPAELEALLL HP ISDAAVV M DE+AGEV
Sbjct: 420  GDIGFIDTDDELFIVDRLKEIIKYKGFQVAPAELEALLLNHPYISDAAVVPMKDEQAGEV 479

Query: 1438 PVAFVVRSNGFTTTEDEIKQFVSKQVIYYKRIYRVFFVDAIPKSPSGKILRKDLRARLAS 1617
            PVAFVVRSNG T TEDEIKQF+SKQV++YKRI RVFF+DAIPKSPSGKILRKDLRARLA+
Sbjct: 480  PVAFVVRSNGSTITEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRARLAA 539

Query: 1618 G 1620
            G
Sbjct: 540  G 540



 Score =  165 bits (418), Expect(2) = 0.0
 Identities = 77/95 (81%), Positives = 89/95 (93%)
 Frame = +3

Query: 66  SVTPKQDIIFRSKLPDIYIPKHLPLHSYCFENISKVSSRPCLINGATGETFTYAQVELIS 245
           ++T ++DIIFRSKLPDIYIPKHLPLHSYCFENISK S+RPCLINGAT E +TY +VELI+
Sbjct: 4   TITKQEDIIFRSKLPDIYIPKHLPLHSYCFENISKFSTRPCLINGATNEVYTYEEVELIA 63

Query: 246 RKVASGLNKLGIKQGDTIMLLLPNSPEYFFAFLGA 350
           RKVA+GL+KLGI+QGDTI+LLLPNSPEY FAFLGA
Sbjct: 64  RKVATGLSKLGIQQGDTILLLLPNSPEYVFAFLGA 98


>sp|O24540.1|4CL_VANPL RecName: Full=4-coumarate--CoA ligase; Short=4CL; AltName:
            Full=4-coumaroyl-CoA synthase
          Length = 553

 Score =  719 bits (1857), Expect(2) = 0.0
 Identities = 364/429 (84%), Positives = 389/429 (90%), Gaps = 5/429 (1%)
 Frame = +1

Query: 358  QVKASNTKLIITQACYVPKVQDYAAEHDIQIVCIDAPPE-----NCLSFSELSGADESEM 522
            Q KASN KLIITQ CYV KV+DYA E+ ++I+ ID         N L FSEL+GADE+EM
Sbjct: 123  QAKASNAKLIITQGCYVDKVKDYACENGVKIISIDTTTTADDAANILHFSELTGADENEM 182

Query: 523  PEVNINSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMICVL 702
            P+V I+ D VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY+HS+DV++CVL
Sbjct: 183  PKVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVL 242

Query: 703  PLFHIYSLNAVLCCGLRAGVAILIMQKFEIVAFLELIQKYKVTIGPFVPPIVLAIAKSPE 882
            PLFHIYSLN+VL CGLRAG  ILIMQKFEIV FLELIQKYKVTIGPFVPPIVLAIAKS  
Sbjct: 243  PLFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTV 302

Query: 883  VDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPY 1062
            VDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEP+
Sbjct: 303  VDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 362

Query: 1063 EIKSGACGTVVRNAEMKIVDPETNTSLPRNQPGEICIRGDQIMKGYLNDPESTERTIDKE 1242
            +IKSGACGTVVRNAEMKIVDPET +SLPRN PGEICIRGDQIMKGYLNDPE+T RTIDKE
Sbjct: 363  DIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKE 422

Query: 1243 GWLHTGDIGYIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVSMIDE 1422
            GWLHTGDIGYIDDDDELFIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVV M DE
Sbjct: 423  GWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDE 482

Query: 1423 KAGEVPVAFVVRSNGFTTTEDEIKQFVSKQVIYYKRIYRVFFVDAIPKSPSGKILRKDLR 1602
             AGEVPVAFVV+SNG   TEDEIKQF+SKQVI+YKRI RVFFV+AIPK+PSGKILRKDLR
Sbjct: 483  AAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLR 542

Query: 1603 ARLASGDLP 1629
            ARLA+  LP
Sbjct: 543  ARLAAAALP 551



 Score =  163 bits (413), Expect(2) = 0.0
 Identities = 79/91 (86%), Positives = 85/91 (93%)
 Frame = +3

Query: 78  KQDIIFRSKLPDIYIPKHLPLHSYCFENISKVSSRPCLINGATGETFTYAQVELISRKVA 257
           K+DIIFRSKLPDIYIPK+LPLHSYCFENISK SSRPCLINGAT E FTYA VELISR+V 
Sbjct: 11  KKDIIFRSKLPDIYIPKNLPLHSYCFENISKFSSRPCLINGATDEIFTYADVELISRRVG 70

Query: 258 SGLNKLGIKQGDTIMLLLPNSPEYFFAFLGA 350
           SGL+KLGIKQGDTIM+LLPNSPE+ FAFLGA
Sbjct: 71  SGLSKLGIKQGDTIMILLPNSPEFVFAFLGA 101


>gb|AEO52694.1| 4-coumarate:CoA ligase [Petunia x hybrida]
          Length = 544

 Score =  718 bits (1854), Expect(2) = 0.0
 Identities = 355/421 (84%), Positives = 387/421 (91%)
 Frame = +1

Query: 358  QVKASNTKLIITQACYVPKVQDYAAEHDIQIVCIDAPPENCLSFSELSGADESEMPEVNI 537
            Q KASN KLIITQAC+V KV+DYA ++++ ++CID+ PE C+ FSEL+ ADE ++P+V I
Sbjct: 121  QAKASNAKLIITQACFVNKVKDYAFDNNLNVICIDSAPEGCIHFSELTQADEHDIPDVKI 180

Query: 538  NSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMICVLPLFHI 717
             SDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NLY+HSEDV++CVLPLFHI
Sbjct: 181  QSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHI 240

Query: 718  YSLNAVLCCGLRAGVAILIMQKFEIVAFLELIQKYKVTIGPFVPPIVLAIAKSPEVDNYD 897
            YSLN+VL CGLR G AILIMQKF+IV F ELI+KYKVTIGPFVPPIVLAIAKSP VDNYD
Sbjct: 241  YSLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYD 300

Query: 898  LSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSG 1077
            LSSVRTVMSGAAPLGKELEDAVR KFPNAKLGQGYGMTEAGPVLAMCLAFAKEP++IKSG
Sbjct: 301  LSSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSG 360

Query: 1078 ACGTVVRNAEMKIVDPETNTSLPRNQPGEICIRGDQIMKGYLNDPESTERTIDKEGWLHT 1257
            ACGTVVRNAEMKIVDP+T  SLPRNQPGEICIRGDQIMKGYLNDP +T RTIDKEGWLHT
Sbjct: 361  ACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHT 420

Query: 1258 GDIGYIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVSMIDEKAGEV 1437
            GDIGYID+DDELFIVDRLKE+IKYKGFQVAPAELEALLL HP ISDAAVV M DE+AGEV
Sbjct: 421  GDIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEV 480

Query: 1438 PVAFVVRSNGFTTTEDEIKQFVSKQVIYYKRIYRVFFVDAIPKSPSGKILRKDLRARLAS 1617
            PVAFVVRSNG   TEDE+K FVSKQVI+YKRI RVFFV+ +PKSPSGKILRKDLRARLA+
Sbjct: 481  PVAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAA 540

Query: 1618 G 1620
            G
Sbjct: 541  G 541



 Score =  154 bits (390), Expect(2) = 0.0
 Identities = 74/97 (76%), Positives = 84/97 (86%)
 Frame = +3

Query: 60  MGSVTPKQDIIFRSKLPDIYIPKHLPLHSYCFENISKVSSRPCLINGATGETFTYAQVEL 239
           M + T + D+IFRSKLPDIYIPKHLPLHSYCFENIS+ SSRPCLINGA    +TYA VEL
Sbjct: 3   METETNQGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVEL 62

Query: 240 ISRKVASGLNKLGIKQGDTIMLLLPNSPEYFFAFLGA 350
            SRKVA+GLNKLGI+Q DTIM+LLPNSPE+ FAF+GA
Sbjct: 63  TSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGA 99