BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002744
         (2236 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAC49931.1| cytosine-5 DNA methyltransferase [Pisum sativum]       491   e-136
ref|XP_003619753.1| DNA (cytosine-5)-methyltransferase [Medicago...   488   e-135
gb|AAC39355.1| Met1-type cytosine DNA-methyltransferase [Daucus ...   390   e-106
gb|AAC39356.1| Met2-type cytosine DNA-methyltransferase [Daucus ...   372   e-100
emb|CBI27718.3| unnamed protein product [Vitis vinifera]              359   2e-96

>gb|AAC49931.1| cytosine-5 DNA methyltransferase [Pisum sativum]
          Length = 1554

 Score =  491 bits (1265), Expect = e-136
 Identities = 259/450 (57%), Positives = 300/450 (66%), Gaps = 1/450 (0%)
 Frame = +1

Query: 889  SPHYFGTERVVKETFKGGRNVGLKAFVICQLLEIIVPKTPKQADLHSTEVKVRRFYRPED 1068
            SP  F  E++ + T K GRNVGLKAFV+CQ+LEII  K  KQA++ STE+KVRRF+RPED
Sbjct: 944  SPFEF-EEKIEQGTHKSGRNVGLKAFVVCQVLEIIAKKETKQAEIKSTELKVRRFFRPED 1002

Query: 1069 VSDEKAYCSDIREVYYSEEYHTLSVKTIEGRCEVRRKSDLPSCDTPTVYEHVFFCEHLYD 1248
            VS EKAYCSD++EVY+S+E +T+SV+++EG+CEVR+K D+P    P  + +VFFCE LYD
Sbjct: 1003 VSSEKAYCSDVQEVYFSDETYTISVQSVEGKCEVRKKIDIPEGSAPGAFHNVFFCELLYD 1062

Query: 1249 PSKGSLKQLPLNTKLRYSSAKSAFDSSLRKNKGKYKEGEN-DSDAEKSEENCLATLDIFA 1425
            P+ GSLK+LP + K+                  KY  G   D+ A K +  C     I  
Sbjct: 1063 PATGSLKKLPSHIKV------------------KYSSGPTADNAARKKKGKCKEGDSISV 1104

Query: 1426 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPESLM 1605
                                                                   PE+L+
Sbjct: 1105 PDIKSKTSNENRLATLDIFAGCGALSEGLHKSGASSTKWAIEYEEPAGNAFKANHPEALV 1164

Query: 1606 FINNCNVILKAIMDKTGDADECISTPXXXXXXXXXXXXDIKSLPLPGQVDFINGGPPCQG 1785
            FINNCNVIL+AIM+K GD DECIST             D+ SLPLPGQVDFINGGPPCQG
Sbjct: 1165 FINNCNVILRAIMEKCGDIDECISTAEAAELASKLDDKDLNSLPLPGQVDFINGGPPCQG 1224

Query: 1786 FSGMNRFNQSTWSKVQCEMILAFLSFADYYRPKYFLLENVRNFVSFNKGQTFSLTIASFL 1965
            FSGMNRFN STWSKVQCEMILAFLSFADY+RP+YFLLENVRNFVSFNKGQTF LT+AS L
Sbjct: 1225 FSGMNRFNTSTWSKVQCEMILAFLSFADYFRPRYFLLENVRNFVSFNKGQTFRLTLASLL 1284

Query: 1966 DMGYQVRFGILEAGAFGVPQSRKRAFIWAASPDVTLPEWPEPMHVFAATELKIALPENKY 2145
            +MGYQVRFGILEAGAFGV QSRKRAFIWAASP+  LPEWPEPMHVF+A ELKI L EN  
Sbjct: 1285 EMGYQVRFGILEAGAFGVSQSRKRAFIWAASPEDVLPEWPEPMHVFSAPELKITLAENVQ 1344

Query: 2146 YAAVRSTQTGAPFRAITVRDTIGDLPMVSN 2235
            YAAV ST  GAP RAITVRDTIG+LP V N
Sbjct: 1345 YAAVCSTANGAPLRAITVRDTIGELPAVGN 1374


>ref|XP_003619753.1| DNA (cytosine-5)-methyltransferase [Medicago truncatula]
            gi|355494768|gb|AES75971.1| DNA
            (cytosine-5)-methyltransferase [Medicago truncatula]
          Length = 1573

 Score =  488 bits (1256), Expect = e-135
 Identities = 255/450 (56%), Positives = 303/450 (67%), Gaps = 1/450 (0%)
 Frame = +1

Query: 889  SPHYFGTERVVKETFKGGRNVGLKAFVICQLLEIIVPKTPKQADLHSTEVKVRRFYRPED 1068
            SP  F  E++ + T K GRNVGL+A+VICQ+LEII  K  KQA++ ST++KVRRF+RPED
Sbjct: 963  SPFEF-EEKIEQGTHKSGRNVGLRAYVICQVLEIIAKKETKQAEIKSTDIKVRRFFRPED 1021

Query: 1069 VSDEKAYCSDIREVYYSEEYHTLSVKTIEGRCEVRRKSDLPSCDTPTVYEHVFFCEHLYD 1248
            VS EKAYCSD++EVYYS+E  T+SV++IEG+CEVR+K D+P    P ++ ++FFCE LYD
Sbjct: 1022 VSSEKAYCSDVQEVYYSDEACTISVQSIEGKCEVRKKIDVPEGCAPGIFHNIFFCELLYD 1081

Query: 1249 PSKGSLKQLPLNTKLRYSSAKSAFDSSLRKNKGKYKEGEN-DSDAEKSEENCLATLDIFA 1425
            P+ G+LK+LP + K+                  KY  G   D+ A K +  C    +I  
Sbjct: 1082 PATGALKKLPAHIKV------------------KYSTGHTADAAARKKKGKCKEGDNISE 1123

Query: 1426 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPESLM 1605
                                                                   PE+L+
Sbjct: 1124 PDPERKTSNEKRLATLDIFAGCGGLSEGLHQSGVSSTKWAIEYEEPAGNAFKANHPEALV 1183

Query: 1606 FINNCNVILKAIMDKTGDADECISTPXXXXXXXXXXXXDIKSLPLPGQVDFINGGPPCQG 1785
            FINNCNVIL+AIM+K GD D+CI+T             D+ +LPLPGQVDFINGGPPCQG
Sbjct: 1184 FINNCNVILRAIMEKCGDIDDCITTTEAAELASKLDEKDLSNLPLPGQVDFINGGPPCQG 1243

Query: 1786 FSGMNRFNQSTWSKVQCEMILAFLSFADYYRPKYFLLENVRNFVSFNKGQTFSLTIASFL 1965
            FSGMNRFN STWSKVQCEMILAFLSFADY+RP+YFLLENVRNFVSFNKGQTF LT+AS L
Sbjct: 1244 FSGMNRFNTSTWSKVQCEMILAFLSFADYFRPRYFLLENVRNFVSFNKGQTFRLTLASLL 1303

Query: 1966 DMGYQVRFGILEAGAFGVPQSRKRAFIWAASPDVTLPEWPEPMHVFAATELKIALPENKY 2145
            +MGYQVRFGILEAGAFGV QSRKRAFIWAASP+  LPEWPEPMHVF+A ELKI L EN  
Sbjct: 1304 EMGYQVRFGILEAGAFGVSQSRKRAFIWAASPEDVLPEWPEPMHVFSAPELKITLSENVQ 1363

Query: 2146 YAAVRSTQTGAPFRAITVRDTIGDLPMVSN 2235
            YAAVRST  GAP RAITVRDTIG+LP+V N
Sbjct: 1364 YAAVRSTANGAPLRAITVRDTIGELPVVGN 1393


>gb|AAC39355.1| Met1-type cytosine DNA-methyltransferase [Daucus carota]
          Length = 1545

 Score =  390 bits (1003), Expect = e-106
 Identities = 190/215 (88%), Positives = 197/215 (91%)
 Frame = +1

Query: 1591 PESLMFINNCNVILKAIMDKTGDADECISTPXXXXXXXXXXXXDIKSLPLPGQVDFINGG 1770
            PESLMFINNCNVILKAIMDKTGDAD+CISTP            +IK+LPLPGQVDFINGG
Sbjct: 1151 PESLMFINNCNVILKAIMDKTGDADDCISTPEAAELAAKLSEEEIKNLPLPGQVDFINGG 1210

Query: 1771 PPCQGFSGMNRFNQSTWSKVQCEMILAFLSFADYYRPKYFLLENVRNFVSFNKGQTFSLT 1950
            PPCQGFSGMNRFNQS+WSKVQCEMILAFLSFADYYRPKYFLLENVR FVSFNKGQTF L 
Sbjct: 1211 PPCQGFSGMNRFNQSSWSKVQCEMILAFLSFADYYRPKYFLLENVRTFVSFNKGQTFRLA 1270

Query: 1951 IASFLDMGYQVRFGILEAGAFGVPQSRKRAFIWAASPDVTLPEWPEPMHVFAATELKIAL 2130
            IAS LDMGYQVRFGILEAGA+GVPQSRKRAFIWAASP+ TLPEWPEPMHVFAA ELKIAL
Sbjct: 1271 IASLLDMGYQVRFGILEAGAYGVPQSRKRAFIWAASPEETLPEWPEPMHVFAAPELKIAL 1330

Query: 2131 PENKYYAAVRSTQTGAPFRAITVRDTIGDLPMVSN 2235
            PENKYYAAVRSTQTGAPFR+ITVRDTIGDLPMVSN
Sbjct: 1331 PENKYYAAVRSTQTGAPFRSITVRDTIGDLPMVSN 1365



 Score =  293 bits (751), Expect = 1e-76
 Identities = 139/179 (77%), Positives = 157/179 (87%)
 Frame = +1

Query: 889  SPHYFGTERVVKETFKGGRNVGLKAFVICQLLEIIVPKTPKQADLHSTEVKVRRFYRPED 1068
            SP  F TERV  ETFKGGRNVGLKA+ ICQLLEIIVPK PKQA+ HSTE+KVRRFYRPED
Sbjct: 937  SPQQFSTERVGNETFKGGRNVGLKAYAICQLLEIIVPKAPKQAEPHSTEIKVRRFYRPED 996

Query: 1069 VSDEKAYCSDIREVYYSEEYHTLSVKTIEGRCEVRRKSDLPSCDTPTVYEHVFFCEHLYD 1248
            +SDEKAYCSDIREVYYSEE HT+  +T+EGRCEVR+K+DLPSCD PT+++HVFFCE+LYD
Sbjct: 997  ISDEKAYCSDIREVYYSEETHTIDAETVEGRCEVRKKNDLPSCDAPTIFDHVFFCEYLYD 1056

Query: 1249 PSKGSLKQLPLNTKLRYSSAKSAFDSSLRKNKGKYKEGENDSDAEKSEENCLATLDIFA 1425
            P+KGSLKQLP N KLRYS+ K A  SSLRKNKGK KEGE+D D+ KS+ NCLATLDIFA
Sbjct: 1057 PAKGSLKQLPPNIKLRYSAVKGAHVSSLRKNKGKCKEGEDDLDSLKSKVNCLATLDIFA 1115


>gb|AAC39356.1| Met2-type cytosine DNA-methyltransferase [Daucus carota]
          Length = 1761

 Score =  372 bits (956), Expect = e-100
 Identities = 181/216 (83%), Positives = 194/216 (89%), Gaps = 1/216 (0%)
 Frame = +1

Query: 1591 PESLMFINNCNVILKAIMDKTGDADECISTPXXXXXXXXXXXXDIKSLPLPGQVDFINGG 1770
            P++ MFINNCNVILKAIMDK+GDAD+CISTP            ++K+LPLPGQVDFINGG
Sbjct: 1366 PDTTMFINNCNVILKAIMDKSGDADDCISTPEAADLAAKLSEEELKNLPLPGQVDFINGG 1425

Query: 1771 PPCQGFSGMNRFNQSTWSKVQCEMILAFLSFADYYRPKYFLLENVRNFVSFNKGQTFSLT 1950
            PPCQGFSGMNRFNQS+WSKVQCEMILAFLSFADYYRPKYFLLENVRNFVSFNKGQTF L 
Sbjct: 1426 PPCQGFSGMNRFNQSSWSKVQCEMILAFLSFADYYRPKYFLLENVRNFVSFNKGQTFRLA 1485

Query: 1951 IASFLDMGYQVRFGILEAGAFGVPQSRKRAFIWAASPDVTLP-EWPEPMHVFAATELKIA 2127
            IAS L+MGYQVRFGILEAGAFGVPQSRKRAFIWAASP+ TLP  WPEPMHVFAA ELK+A
Sbjct: 1486 IASLLEMGYQVRFGILEAGAFGVPQSRKRAFIWAASPEETLPGSWPEPMHVFAAPELKVA 1545

Query: 2128 LPENKYYAAVRSTQTGAPFRAITVRDTIGDLPMVSN 2235
            LP NK+YAAVRSTQ GAPFRAITVRDTIGDLPMV+N
Sbjct: 1546 LPGNKHYAAVRSTQAGAPFRAITVRDTIGDLPMVTN 1581



 Score =  301 bits (772), Expect = 4e-79
 Identities = 144/179 (80%), Positives = 160/179 (89%)
 Frame = +1

Query: 889  SPHYFGTERVVKETFKGGRNVGLKAFVICQLLEIIVPKTPKQADLHSTEVKVRRFYRPED 1068
            SP  F TERV +ETFKGGRNVGLKAF ICQLLE++VPK P+QAD  STEVKVRRFYRPED
Sbjct: 1152 SPDQFATERVGQETFKGGRNVGLKAFAICQLLEVVVPKKPQQADDSSTEVKVRRFYRPED 1211

Query: 1069 VSDEKAYCSDIREVYYSEEYHTLSVKTIEGRCEVRRKSDLPSCDTPTVYEHVFFCEHLYD 1248
            +SDEKAYCSDIREVYYSEE HTL V+ IEGRCEVR+KSDLP+CD PT+YEHVF+CE+LYD
Sbjct: 1212 ISDEKAYCSDIREVYYSEETHTLLVEAIEGRCEVRKKSDLPTCDAPTIYEHVFYCEYLYD 1271

Query: 1249 PSKGSLKQLPLNTKLRYSSAKSAFDSSLRKNKGKYKEGENDSDAEKSEENCLATLDIFA 1425
            P KGSLKQLP N KLRYS+ K A+DSSLRKNKGK KEGE+D +AEKS+ENCLATLDIFA
Sbjct: 1272 PHKGSLKQLPSNIKLRYSTVKGAYDSSLRKNKGKCKEGEDDLEAEKSKENCLATLDIFA 1330


>emb|CBI27718.3| unnamed protein product [Vitis vinifera]
          Length = 1429

 Score =  359 bits (922), Expect = 2e-96
 Identities = 175/215 (81%), Positives = 186/215 (86%)
 Frame = +1

Query: 1591 PESLMFINNCNVILKAIMDKTGDADECISTPXXXXXXXXXXXXDIKSLPLPGQVDFINGG 1770
            PESLMFINNCNVIL+A+M+K GD D+CIST             DI +LPLPGQVDFINGG
Sbjct: 1034 PESLMFINNCNVILRAVMEKCGDDDDCISTSEAAELAAALGEKDINNLPLPGQVDFINGG 1093

Query: 1771 PPCQGFSGMNRFNQSTWSKVQCEMILAFLSFADYYRPKYFLLENVRNFVSFNKGQTFSLT 1950
            PPCQGFSGMNRFNQSTWSKVQCEMILAFLSFADY+RPK+FLLENVRNFVSFNKGQTF LT
Sbjct: 1094 PPCQGFSGMNRFNQSTWSKVQCEMILAFLSFADYFRPKFFLLENVRNFVSFNKGQTFRLT 1153

Query: 1951 IASFLDMGYQVRFGILEAGAFGVPQSRKRAFIWAASPDVTLPEWPEPMHVFAATELKIAL 2130
            +AS L+MGYQVRFGILEAGA+GV QSRKRAFIWAASP+ TLPEWPEPMHVFA  ELKI L
Sbjct: 1154 LASLLEMGYQVRFGILEAGAYGVSQSRKRAFIWAASPEETLPEWPEPMHVFAVPELKITL 1213

Query: 2131 PENKYYAAVRSTQTGAPFRAITVRDTIGDLPMVSN 2235
             EN  YAAVRST TGAPFRAITVRDTIGDLP V N
Sbjct: 1214 SENMQYAAVRSTATGAPFRAITVRDTIGDLPDVKN 1248



 Score =  246 bits (629), Expect = 1e-62
 Identities = 118/183 (64%), Positives = 142/183 (77%), Gaps = 4/183 (2%)
 Frame = +1

Query: 889  SPHYFGTERVVKETFKGGRNVGLKAFVICQLLEIIVPKTPKQADLHSTEVKVRRFYRPED 1068
            SP +F  ER    TFK GRNVGLKA+V+CQ+LEI+VPK PK A+  S +V+VRRF+RPED
Sbjct: 816  SPQHFAAERAETGTFKAGRNVGLKAYVVCQMLEIVVPKVPKIAETKSIQVQVRRFFRPED 875

Query: 1069 VSDEKAYCSDIREVYYSEEYHTLSVKTIEGRCEVRRKSDLPSCDTPTVYEHVFFCEHLYD 1248
            +S EKAYCSDIREVYYSEE H++ V+TIEG+CEV +K DLP CD P +++HVFFCE LYD
Sbjct: 876  ISAEKAYCSDIREVYYSEETHSVPVETIEGKCEVMKKHDLPPCDVPAIFDHVFFCERLYD 935

Query: 1249 PSKGSLKQLPLNTKLRYSSAKSAFDSSLRKNKGKYKEGENDSDAEKS----EENCLATLD 1416
            PSKG LKQLP + KLRYS+ K   D++ RK KGK KEGEND + E+      EN LATLD
Sbjct: 936  PSKGCLKQLPAHIKLRYSARKEVDDAAARKKKGKAKEGENDLEVERQIDAFHENRLATLD 995

Query: 1417 IFA 1425
            IFA
Sbjct: 996  IFA 998