BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002714
         (1549 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283911.1| PREDICTED: pyruvate kinase, cytosolic isozym...   432   e-152
emb|CBI30221.3| unnamed protein product [Vitis vinifera]              432   e-152
ref|XP_002263319.1| PREDICTED: pyruvate kinase, cytosolic isozym...   422   e-152
emb|CBI40200.3| unnamed protein product [Vitis vinifera]              422   e-152
ref|XP_002326407.1| predicted protein [Populus trichocarpa] gi|1...   424   e-150

>ref|XP_002283911.1| PREDICTED: pyruvate kinase, cytosolic isozyme-like [Vitis vinifera]
          Length = 527

 Score =  432 bits (1111), Expect(2) = e-152
 Identities = 219/230 (95%), Positives = 226/230 (98%)
 Frame = +3

Query: 288 EVISTWGVRNNIDFLSLSYTRHAEDVRHAREFLSKLGDLSQTQIFAKIENVEGLNHFDEI 467
           EVISTWGV+N IDFLSLSYTRHAEDVRHAR+ LSKLGDL QTQIFAKIENVEGLNHFDEI
Sbjct: 210 EVISTWGVKNKIDFLSLSYTRHAEDVRHARDHLSKLGDLHQTQIFAKIENVEGLNHFDEI 269

Query: 468 LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAVVTRVVDSMTDNLRPTRAE 647
           LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAV+TRVVDSMTDNLRPTRAE
Sbjct: 270 LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAVITRVVDSMTDNLRPTRAE 329

Query: 648 ATDVANAILDGSDAILLGAETLRGLYPVETISIVGKICAEAEKAYNQDLYFKKTVKYVGE 827
           ATDVANA+LDGSDAILLGAETLRGLYPVETISIVGKICAEAEK +NQDLYFKKTVKYVGE
Sbjct: 330 ATDVANAVLDGSDAILLGAETLRGLYPVETISIVGKICAEAEKVFNQDLYFKKTVKYVGE 389

Query: 828 PMTHLESIASSAVRAAIKVKASVIICFTSSGQAARLIAKYRPTMPVISVV 977
           PMTHLESIASSAVRAAIKVKASVIICFTSSG+AARLIAKYRPTMPV+SVV
Sbjct: 390 PMTHLESIASSAVRAAIKVKASVIICFTSSGRAARLIAKYRPTMPVLSVV 439



 Score =  136 bits (342), Expect(2) = e-152
 Identities = 65/75 (86%), Positives = 73/75 (97%)
 Frame = +1

Query: 1   HGLSKAVKPGDTIFIGQYLFTGSETTSVWLEVTEVKGEDVVCLIKNSATLAGSLYTLHVS 180
           +GL+KAVK GDTIF+GQYLFTGSETTSVWLEV+EVKG+DVVC+IKNSATLAGSL+TLHVS
Sbjct: 136 NGLAKAVKKGDTIFVGQYLFTGSETTSVWLEVSEVKGDDVVCMIKNSATLAGSLFTLHVS 195

Query: 181 QIRIDLPTLTDKDKE 225
           QI IDLPTL+DKDKE
Sbjct: 196 QIHIDLPTLSDKDKE 210



 Score =  127 bits (319), Expect = 8e-27
 Identities = 61/70 (87%), Positives = 67/70 (95%)
 Frame = +2

Query: 1103 ARQTLIVRGLFPLLADPRHPAESTGATNESILKVALDHGKASGIVKPHDRVVVCQKVGDD 1282
            ARQ+LIVRGLFP+LADPRHPAEST ATNES+LKVALDHGKASG++K HDRVVVCQKVGD 
Sbjct: 458  ARQSLIVRGLFPMLADPRHPAESTSATNESVLKVALDHGKASGVIKSHDRVVVCQKVGDA 517

Query: 1283 AVVKIIELED 1312
            +VVKIIELED
Sbjct: 518  SVVKIIELED 527


>emb|CBI30221.3| unnamed protein product [Vitis vinifera]
          Length = 514

 Score =  432 bits (1111), Expect(2) = e-152
 Identities = 219/230 (95%), Positives = 226/230 (98%)
 Frame = +3

Query: 288 EVISTWGVRNNIDFLSLSYTRHAEDVRHAREFLSKLGDLSQTQIFAKIENVEGLNHFDEI 467
           EVISTWGV+N IDFLSLSYTRHAEDVRHAR+ LSKLGDL QTQIFAKIENVEGLNHFDEI
Sbjct: 197 EVISTWGVKNKIDFLSLSYTRHAEDVRHARDHLSKLGDLHQTQIFAKIENVEGLNHFDEI 256

Query: 468 LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAVVTRVVDSMTDNLRPTRAE 647
           LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAV+TRVVDSMTDNLRPTRAE
Sbjct: 257 LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAVITRVVDSMTDNLRPTRAE 316

Query: 648 ATDVANAILDGSDAILLGAETLRGLYPVETISIVGKICAEAEKAYNQDLYFKKTVKYVGE 827
           ATDVANA+LDGSDAILLGAETLRGLYPVETISIVGKICAEAEK +NQDLYFKKTVKYVGE
Sbjct: 317 ATDVANAVLDGSDAILLGAETLRGLYPVETISIVGKICAEAEKVFNQDLYFKKTVKYVGE 376

Query: 828 PMTHLESIASSAVRAAIKVKASVIICFTSSGQAARLIAKYRPTMPVISVV 977
           PMTHLESIASSAVRAAIKVKASVIICFTSSG+AARLIAKYRPTMPV+SVV
Sbjct: 377 PMTHLESIASSAVRAAIKVKASVIICFTSSGRAARLIAKYRPTMPVLSVV 426



 Score =  136 bits (342), Expect(2) = e-152
 Identities = 65/75 (86%), Positives = 73/75 (97%)
 Frame = +1

Query: 1   HGLSKAVKPGDTIFIGQYLFTGSETTSVWLEVTEVKGEDVVCLIKNSATLAGSLYTLHVS 180
           +GL+KAVK GDTIF+GQYLFTGSETTSVWLEV+EVKG+DVVC+IKNSATLAGSL+TLHVS
Sbjct: 123 NGLAKAVKKGDTIFVGQYLFTGSETTSVWLEVSEVKGDDVVCMIKNSATLAGSLFTLHVS 182

Query: 181 QIRIDLPTLTDKDKE 225
           QI IDLPTL+DKDKE
Sbjct: 183 QIHIDLPTLSDKDKE 197



 Score =  127 bits (319), Expect = 8e-27
 Identities = 61/70 (87%), Positives = 67/70 (95%)
 Frame = +2

Query: 1103 ARQTLIVRGLFPLLADPRHPAESTGATNESILKVALDHGKASGIVKPHDRVVVCQKVGDD 1282
            ARQ+LIVRGLFP+LADPRHPAEST ATNES+LKVALDHGKASG++K HDRVVVCQKVGD 
Sbjct: 445  ARQSLIVRGLFPMLADPRHPAESTSATNESVLKVALDHGKASGVIKSHDRVVVCQKVGDA 504

Query: 1283 AVVKIIELED 1312
            +VVKIIELED
Sbjct: 505  SVVKIIELED 514


>ref|XP_002263319.1| PREDICTED: pyruvate kinase, cytosolic isozyme-like [Vitis vinifera]
          Length = 527

 Score =  422 bits (1085), Expect(2) = e-152
 Identities = 212/230 (92%), Positives = 222/230 (96%)
 Frame = +3

Query: 288 EVISTWGVRNNIDFLSLSYTRHAEDVRHAREFLSKLGDLSQTQIFAKIENVEGLNHFDEI 467
           EVISTWGV+N IDFLSLSYTRHAEDVRHAREFLSKLGDL+QTQIFAKIEN+EGLNHFDEI
Sbjct: 210 EVISTWGVQNKIDFLSLSYTRHAEDVRHAREFLSKLGDLNQTQIFAKIENIEGLNHFDEI 269

Query: 468 LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAVVTRVVDSMTDNLRPTRAE 647
           LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAV+TRVVDSMTDNLRPTRAE
Sbjct: 270 LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAVITRVVDSMTDNLRPTRAE 329

Query: 648 ATDVANAILDGSDAILLGAETLRGLYPVETISIVGKICAEAEKAYNQDLYFKKTVKYVGE 827
           ATDVANA+LDGSDAILLGAETLRGLYPVETIS V KICAEAEK +NQD YFKK VK+VGE
Sbjct: 330 ATDVANAVLDGSDAILLGAETLRGLYPVETISTVNKICAEAEKVFNQDFYFKKAVKHVGE 389

Query: 828 PMTHLESIASSAVRAAIKVKASVIICFTSSGQAARLIAKYRPTMPVISVV 977
           PMTHLESIASSAVRAAI VKASVI+CFTS+G+AARLI KYRPTMPVISVV
Sbjct: 390 PMTHLESIASSAVRAAISVKASVIVCFTSTGKAARLIGKYRPTMPVISVV 439



 Score =  143 bits (361), Expect(2) = e-152
 Identities = 72/74 (97%), Positives = 72/74 (97%)
 Frame = +1

Query: 4   GLSKAVKPGDTIFIGQYLFTGSETTSVWLEVTEVKGEDVVCLIKNSATLAGSLYTLHVSQ 183
           GLSKAVK GDTIFIGQYLFTGSETTSVWLEVTEV GEDVVCLIKNSATLAGSLYTLHVSQ
Sbjct: 137 GLSKAVKKGDTIFIGQYLFTGSETTSVWLEVTEVDGEDVVCLIKNSATLAGSLYTLHVSQ 196

Query: 184 IRIDLPTLTDKDKE 225
           IRIDLPTLTDKDKE
Sbjct: 197 IRIDLPTLTDKDKE 210



 Score =  123 bits (308), Expect = 2e-25
 Identities = 58/70 (82%), Positives = 66/70 (94%)
 Frame = +2

Query: 1103 ARQTLIVRGLFPLLADPRHPAESTGATNESILKVALDHGKASGIVKPHDRVVVCQKVGDD 1282
            ARQ++IVRG+FP+LADPRHPAEST ATNESILKVALDHGKA G++K HDR+VVCQKVGD 
Sbjct: 458  ARQSVIVRGIFPMLADPRHPAESTNATNESILKVALDHGKAFGVIKSHDRIVVCQKVGDA 517

Query: 1283 AVVKIIELED 1312
            +VVKIIELED
Sbjct: 518  SVVKIIELED 527


>emb|CBI40200.3| unnamed protein product [Vitis vinifera]
          Length = 514

 Score =  422 bits (1085), Expect(2) = e-152
 Identities = 212/230 (92%), Positives = 222/230 (96%)
 Frame = +3

Query: 288 EVISTWGVRNNIDFLSLSYTRHAEDVRHAREFLSKLGDLSQTQIFAKIENVEGLNHFDEI 467
           EVISTWGV+N IDFLSLSYTRHAEDVRHAREFLSKLGDL+QTQIFAKIEN+EGLNHFDEI
Sbjct: 197 EVISTWGVQNKIDFLSLSYTRHAEDVRHAREFLSKLGDLNQTQIFAKIENIEGLNHFDEI 256

Query: 468 LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAVVTRVVDSMTDNLRPTRAE 647
           LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAV+TRVVDSMTDNLRPTRAE
Sbjct: 257 LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAVITRVVDSMTDNLRPTRAE 316

Query: 648 ATDVANAILDGSDAILLGAETLRGLYPVETISIVGKICAEAEKAYNQDLYFKKTVKYVGE 827
           ATDVANA+LDGSDAILLGAETLRGLYPVETIS V KICAEAEK +NQD YFKK VK+VGE
Sbjct: 317 ATDVANAVLDGSDAILLGAETLRGLYPVETISTVNKICAEAEKVFNQDFYFKKAVKHVGE 376

Query: 828 PMTHLESIASSAVRAAIKVKASVIICFTSSGQAARLIAKYRPTMPVISVV 977
           PMTHLESIASSAVRAAI VKASVI+CFTS+G+AARLI KYRPTMPVISVV
Sbjct: 377 PMTHLESIASSAVRAAISVKASVIVCFTSTGKAARLIGKYRPTMPVISVV 426



 Score =  143 bits (361), Expect(2) = e-152
 Identities = 72/74 (97%), Positives = 72/74 (97%)
 Frame = +1

Query: 4   GLSKAVKPGDTIFIGQYLFTGSETTSVWLEVTEVKGEDVVCLIKNSATLAGSLYTLHVSQ 183
           GLSKAVK GDTIFIGQYLFTGSETTSVWLEVTEV GEDVVCLIKNSATLAGSLYTLHVSQ
Sbjct: 124 GLSKAVKKGDTIFIGQYLFTGSETTSVWLEVTEVDGEDVVCLIKNSATLAGSLYTLHVSQ 183

Query: 184 IRIDLPTLTDKDKE 225
           IRIDLPTLTDKDKE
Sbjct: 184 IRIDLPTLTDKDKE 197



 Score =  123 bits (308), Expect = 2e-25
 Identities = 58/70 (82%), Positives = 66/70 (94%)
 Frame = +2

Query: 1103 ARQTLIVRGLFPLLADPRHPAESTGATNESILKVALDHGKASGIVKPHDRVVVCQKVGDD 1282
            ARQ++IVRG+FP+LADPRHPAEST ATNESILKVALDHGKA G++K HDR+VVCQKVGD 
Sbjct: 445  ARQSVIVRGIFPMLADPRHPAESTNATNESILKVALDHGKAFGVIKSHDRIVVCQKVGDA 504

Query: 1283 AVVKIIELED 1312
            +VVKIIELED
Sbjct: 505  SVVKIIELED 514


>ref|XP_002326407.1| predicted protein [Populus trichocarpa] gi|118480989|gb|ABK92448.1|
           unknown [Populus trichocarpa]
           gi|222833600|gb|EEE72077.1| predicted protein [Populus
           trichocarpa]
          Length = 527

 Score =  424 bits (1091), Expect(2) = e-150
 Identities = 214/230 (93%), Positives = 225/230 (97%)
 Frame = +3

Query: 288 EVISTWGVRNNIDFLSLSYTRHAEDVRHAREFLSKLGDLSQTQIFAKIENVEGLNHFDEI 467
           EVISTWGV+N IDFLSLSYTRHAEDVR+ARE+LSKLGDL QTQIFAKIEN+EGL HFDEI
Sbjct: 210 EVISTWGVQNKIDFLSLSYTRHAEDVRNAREYLSKLGDLYQTQIFAKIENIEGLTHFDEI 269

Query: 468 LQEADGIILSRGNLGIDLPPEKVFLFQKAAVYKCNMAGKPAVVTRVVDSMTDNLRPTRAE 647
           LQEADGIILSRGNLGIDLPPEKVFLFQKAA+YKCNMAGKPAVVTRVVDSMTDNLRPTRAE
Sbjct: 270 LQEADGIILSRGNLGIDLPPEKVFLFQKAALYKCNMAGKPAVVTRVVDSMTDNLRPTRAE 329

Query: 648 ATDVANAILDGSDAILLGAETLRGLYPVETISIVGKICAEAEKAYNQDLYFKKTVKYVGE 827
           ATDVANA+LDGSDAILLGAETLRGLYP+ETIS VGKICAEAEK +NQDLYFKKTVKYVGE
Sbjct: 330 ATDVANAVLDGSDAILLGAETLRGLYPIETISTVGKICAEAEKVFNQDLYFKKTVKYVGE 389

Query: 828 PMTHLESIASSAVRAAIKVKASVIICFTSSGQAARLIAKYRPTMPVISVV 977
           PMTHLESIASSAVRAAIKVK+SVIICFTSSG+AARLIAKYRPTMPV+SVV
Sbjct: 390 PMTHLESIASSAVRAAIKVKSSVIICFTSSGRAARLIAKYRPTMPVLSVV 439



 Score =  134 bits (337), Expect(2) = e-150
 Identities = 65/74 (87%), Positives = 71/74 (95%)
 Frame = +1

Query: 4   GLSKAVKPGDTIFIGQYLFTGSETTSVWLEVTEVKGEDVVCLIKNSATLAGSLYTLHVSQ 183
           GLSKAVK GDTIF+GQYLFTGSETTSVWLEV+EVKG DVVC+IKNSATLAGSL+TLH SQ
Sbjct: 137 GLSKAVKHGDTIFVGQYLFTGSETTSVWLEVSEVKGNDVVCVIKNSATLAGSLFTLHASQ 196

Query: 184 IRIDLPTLTDKDKE 225
           IRI+LPTL+DKDKE
Sbjct: 197 IRIELPTLSDKDKE 210



 Score =  127 bits (319), Expect = 8e-27
 Identities = 61/70 (87%), Positives = 67/70 (95%)
 Frame = +2

Query: 1103 ARQTLIVRGLFPLLADPRHPAESTGATNESILKVALDHGKASGIVKPHDRVVVCQKVGDD 1282
            ARQ+LIVRGLFP+LADPRHPAEST ATNESILKVALDHGKASG++K HDRVV+CQKVGD 
Sbjct: 458  ARQSLIVRGLFPMLADPRHPAESTSATNESILKVALDHGKASGVIKSHDRVVICQKVGDA 517

Query: 1283 AVVKIIELED 1312
            +VVKIIELED
Sbjct: 518  SVVKIIELED 527