BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002620
         (1734 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002273005.2| PREDICTED: serine/threonine-protein kinase W...   557   e-156
emb|CBI34567.3| unnamed protein product [Vitis vinifera]              553   e-155
ref|XP_002518890.1| kinase, putative [Ricinus communis] gi|22354...   548   e-153
ref|XP_004143946.1| PREDICTED: serine/threonine-protein kinase W...   539   e-150
ref|XP_003601461.1| Serine/threonine protein kinase WNK8 [Medica...   536   e-150

>ref|XP_002273005.2| PREDICTED: serine/threonine-protein kinase WNK8-like [Vitis vinifera]
          Length = 645

 Score =  557 bits (1436), Expect = e-156
 Identities = 276/428 (64%), Positives = 333/428 (77%), Gaps = 25/428 (5%)
 Frame = -2

Query: 1241 DPAFVERSPDNRYIRYNKILGRGAFKTVYKGFDEIDGIEVAWSVVDMDDLLLSPENLERI 1062
            D  F E+ P  RY+RYN+ LG+GAFKTVYK FDE+DGIEVAW  V+++DLL SP+ LER+
Sbjct: 10   DSDFEEKDPTGRYVRYNEFLGKGAFKTVYKAFDEVDGIEVAWGQVEIEDLLQSPQQLERL 69

Query: 1061 YSEVHLLETLRHENVIKFYIFWEDNERKTINMITELFSSGSLRQYRKNHMNVDMKAIKNW 882
            YSEVHLL++L+H+N+IKFY  W D+  KTIN+ITELF+SGSLRQYRK H NVD+KAIKNW
Sbjct: 70   YSEVHLLKSLKHDNIIKFYNSWVDDTNKTINLITELFTSGSLRQYRKKHKNVDLKAIKNW 129

Query: 881  AKQILLGLNYLHSQSPPIIHRDLKCDNIFVNGSHGEVKIGDLGFAKIMHQSTAHSVVGTP 702
            AKQIL GL+YLHS +PPIIHRDLKCDNIFVNG++GEVKIGDLG A +M Q TA SV+GTP
Sbjct: 130  AKQILRGLHYLHSHNPPIIHRDLKCDNIFVNGNNGEVKIGDLGLAIVMQQPTARSVIGTP 189

Query: 701  EFMAPELYDEEYNELVDIYSFGLCILELITCEYPYKECKNQAQIYKKVTSGIKPASLGKI 522
            EFMAPELY+EEYNELVDIYSFG+CILEL+TCEYPY ECKN AQIYKKV+SGIKPASLGK+
Sbjct: 190  EFMAPELYEEEYNELVDIYSFGMCILELVTCEYPYNECKNPAQIYKKVSSGIKPASLGKV 249

Query: 521  NDPQAKQFIEKCIVAVSQRLSASELLKDPFLSSESSISP--------------------- 405
            +DPQ KQFIEKC+V  S RLSA ELLKD F ++E+S  P                     
Sbjct: 250  SDPQVKQFIEKCLVPASLRLSAQELLKDAFFATENSKEPVYNHMHVDDSLQSSNFMPNLM 309

Query: 404  ----QKLDPVNMVIDTNCDKLSSSTNAESSIETIHAWSTEFQRFNERNEYLLRGEKNDER 237
                 +L P++M  D N  KLS ST+ +S   T H  + +F+RFN+ N + LRGEK D+ 
Sbjct: 310  NLPKPELQPMDM--DPNYKKLSVSTHMKSISGTPHFRALQFERFNKNNLFKLRGEKIDDN 367

Query: 236  TVSLSLRITDLRGQVRNIHFMFYLDADTTSSLAGEMVEQLDLWNEDVAFIAELIDDLIFS 57
            ++S++L I D  G+ +NIHF FYLD+DT  S+AGEMVEQLDL+NEDVA IAELID +I  
Sbjct: 368  SISMTLHIADPCGRAKNIHFAFYLDSDTALSIAGEMVEQLDLYNEDVAVIAELIDVMISE 427

Query: 56   LVPTWKPS 33
            LVPTWKP+
Sbjct: 428  LVPTWKPA 435


>emb|CBI34567.3| unnamed protein product [Vitis vinifera]
          Length = 596

 Score =  553 bits (1426), Expect = e-155
 Identities = 270/403 (66%), Positives = 327/403 (81%)
 Frame = -2

Query: 1241 DPAFVERSPDNRYIRYNKILGRGAFKTVYKGFDEIDGIEVAWSVVDMDDLLLSPENLERI 1062
            D  F E+ P  RY+RYN+ LG+GAFKTVYK FDE+DGIEVAW  V+++DLL SP+ LER+
Sbjct: 149  DSDFEEKDPTGRYVRYNEFLGKGAFKTVYKAFDEVDGIEVAWGQVEIEDLLQSPQQLERL 208

Query: 1061 YSEVHLLETLRHENVIKFYIFWEDNERKTINMITELFSSGSLRQYRKNHMNVDMKAIKNW 882
            YSEVHLL++L+H+N+IKFY  W D+  KTIN+ITELF+SGSLRQYRK H NVD+KAIKNW
Sbjct: 209  YSEVHLLKSLKHDNIIKFYNSWVDDTNKTINLITELFTSGSLRQYRKKHKNVDLKAIKNW 268

Query: 881  AKQILLGLNYLHSQSPPIIHRDLKCDNIFVNGSHGEVKIGDLGFAKIMHQSTAHSVVGTP 702
            AKQIL GL+YLHS +PPIIHRDLKCDNIFVNG++GEVKIGDLG A +M Q TA SV+GTP
Sbjct: 269  AKQILRGLHYLHSHNPPIIHRDLKCDNIFVNGNNGEVKIGDLGLAIVMQQPTARSVIGTP 328

Query: 701  EFMAPELYDEEYNELVDIYSFGLCILELITCEYPYKECKNQAQIYKKVTSGIKPASLGKI 522
            EFMAPELY+EEYNELVDIYSFG+CILEL+TCEYPY ECKN AQIYKKV+SGIKPASLGK+
Sbjct: 329  EFMAPELYEEEYNELVDIYSFGMCILELVTCEYPYNECKNPAQIYKKVSSGIKPASLGKV 388

Query: 521  NDPQAKQFIEKCIVAVSQRLSASELLKDPFLSSESSISPQKLDPVNMVIDTNCDKLSSST 342
            +DPQ KQFIEKC+V  S RLSA ELLKD F ++E+S  P       + + T+    S ST
Sbjct: 389  SDPQVKQFIEKCLVPASLRLSAQELLKDAFFATENSKEP-------LSVSTH---KSMST 438

Query: 341  NAESSIETIHAWSTEFQRFNERNEYLLRGEKNDERTVSLSLRITDLRGQVRNIHFMFYLD 162
            + +S   T H  + +F+RFN+ N + LRGEK D+ ++S++L I D  G+ +NIHF FYLD
Sbjct: 439  HMKSISGTPHFRALQFERFNKNNLFKLRGEKIDDNSISMTLHIADPCGRAKNIHFAFYLD 498

Query: 161  ADTTSSLAGEMVEQLDLWNEDVAFIAELIDDLIFSLVPTWKPS 33
            +DT  S+AGEMVEQLDL+NEDVA IAELID +I  LVPTWKP+
Sbjct: 499  SDTALSIAGEMVEQLDLYNEDVAVIAELIDVMISELVPTWKPA 541


>ref|XP_002518890.1| kinase, putative [Ricinus communis] gi|223541877|gb|EEF43423.1|
            kinase, putative [Ricinus communis]
          Length = 585

 Score =  548 bits (1413), Expect = e-153
 Identities = 271/415 (65%), Positives = 328/415 (79%), Gaps = 17/415 (4%)
 Frame = -2

Query: 1241 DPAFVERSPDNRYIRYNKILGRGAFKTVYKGFDEIDGIEVAWSVVDMDDLLLSPENLERI 1062
            D  F E+ P  RY+RY+++LG+GAFKTVYK FDEI+GIEVAW+ V ++D+L SP+ LER+
Sbjct: 10   DSEFEEKDPTGRYVRYDEVLGKGAFKTVYKAFDEINGIEVAWNQVSVEDVLQSPDQLERL 69

Query: 1061 YSEVHLLETLRHENVIKFYIFWEDNERKTINMITELFSSGSLRQYRKNHMNVDMKAIKNW 882
            YSEVHLL++L+HEN++KFY  W D+  KTINMITELF+SGSLR+YRK H NVD+KAIKNW
Sbjct: 70   YSEVHLLKSLKHENIMKFYNSWVDDNNKTINMITELFTSGSLRKYRKKHKNVDIKAIKNW 129

Query: 881  AKQILLGLNYLHSQSPPIIHRDLKCDNIFVNGSHGEVKIGDLGFAKIMHQSTAHSVVGTP 702
            A+QIL GL+YLHS +PPIIHRDLKCDN+FVNG++GEVKIGDLG A +M Q TA SV+GTP
Sbjct: 130  ARQILRGLHYLHSHNPPIIHRDLKCDNVFVNGNNGEVKIGDLGLATVMQQPTARSVIGTP 189

Query: 701  EFMAPELYDEEYNELVDIYSFGLCILELITCEYPYKECKNQAQIYKKVTSGIKPASLGKI 522
            EFMAPELY+EEYNELVDIYSFG+CILE++TCEYPY ECKN AQIYKKVTSGIKPASL K+
Sbjct: 190  EFMAPELYEEEYNELVDIYSFGMCILEMVTCEYPYSECKNPAQIYKKVTSGIKPASLCKV 249

Query: 521  NDPQAKQFIEKCIVAVSQRLSASELLKDPFLSSES-----------------SISPQKLD 393
            NDP  K+FIEKCIV  S RL A ELLKDPFL++E+                  +S  + +
Sbjct: 250  NDPLVKRFIEKCIVPASMRLPALELLKDPFLATENPKELVFASLHLPDLMLKQVSRGQSE 309

Query: 392  PVNMVIDTNCDKLSSSTNAESSIETIHAWSTEFQRFNERNEYLLRGEKNDERTVSLSLRI 213
               M ID+N  KLS  +  +S  E++H  ++EFQR   +NE+ LRGEKND+ T+SL+LRI
Sbjct: 310  SYPMDIDSNHKKLSVGSCTKSMDESLHFSTSEFQRLTVKNEFRLRGEKNDDNTISLTLRI 369

Query: 212  TDLRGQVRNIHFMFYLDADTTSSLAGEMVEQLDLWNEDVAFIAELIDDLIFSLVP 48
             D  G V+NIHF FYLD+DTT S+A EMVEQLDL NEDVA IAELID LI  LVP
Sbjct: 370  VDRCGGVKNIHFTFYLDSDTTLSIAEEMVEQLDLSNEDVAIIAELIDALILKLVP 424


>ref|XP_004143946.1| PREDICTED: serine/threonine-protein kinase WNK8-like [Cucumis
            sativus] gi|449529646|ref|XP_004171809.1| PREDICTED:
            serine/threonine-protein kinase WNK8-like [Cucumis
            sativus]
          Length = 679

 Score =  539 bits (1388), Expect = e-150
 Identities = 267/421 (63%), Positives = 321/421 (76%), Gaps = 19/421 (4%)
 Frame = -2

Query: 1241 DPAFVERSPDNRYIRYNKILGRGAFKTVYKGFDEIDGIEVAWSVVDMDDLLLSPENLERI 1062
            D   VE+ P  RYIRY++ILG+GAFKTVYK FDE+ GIEVAWS VD++D+L SPE L+R+
Sbjct: 63   DRGIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQLQRL 122

Query: 1061 YSEVHLLETLRHENVIKFYIFWEDNERKTINMITELFSSGSLRQYRKNHMNVDMKAIKNW 882
            YSEVHLL++L+HEN+IKFY +W D++ KTINMITELF+SGSLRQYRK H  VD+KA KNW
Sbjct: 123  YSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRKVDLKAFKNW 182

Query: 881  AKQILLGLNYLHSQSPPIIHRDLKCDNIFVNGSHGEVKIGDLGFAKIMHQSTAHSVVGTP 702
            A+QIL GL YLH  +PPIIHRDLKCDNIFVNG+ GEVKIGDLG A +M Q TA SV+GTP
Sbjct: 183  ARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSVIGTP 242

Query: 701  EFMAPELYDEEYNELVDIYSFGLCILELITCEYPYKECKNQAQIYKKVTSGIKPASLGKI 522
            EFMAPELYDE+YNELVDIYSFG+C+LE++TCEYPY ECKN AQI+KKVTSGIKPASL K+
Sbjct: 243  EFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPASLEKV 302

Query: 521  NDPQAKQFIEKCIVAVSQRLSASELLKDPFLSSES-----------------SISPQKLD 393
             DPQ KQFIEKC+V  S RL ASELLKDPFL++ES                 S++P  L 
Sbjct: 303  LDPQVKQFIEKCLVPASTRLPASELLKDPFLAAESPKDNSSELSRSLNEHFKSVNPPLLG 362

Query: 392  PVNMVIDTNCDKLSSS--TNAESSIETIHAWSTEFQRFNERNEYLLRGEKNDERTVSLSL 219
               M  D NC KLS S  ++ +S+    H  + E QR  E NE  L+G+  D  T+S  L
Sbjct: 363  SHPMETDHNCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGDMTDHNTMSFHL 422

Query: 218  RITDLRGQVRNIHFMFYLDADTTSSLAGEMVEQLDLWNEDVAFIAELIDDLIFSLVPTWK 39
            RI +L G+ RNIHF FYLD+DT+ ++A EMVEQL+L NED   IA+LID+LI   VP+WK
Sbjct: 423  RIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDELIAKFVPSWK 482

Query: 38   P 36
            P
Sbjct: 483  P 483


>ref|XP_003601461.1| Serine/threonine protein kinase WNK8 [Medicago truncatula]
            gi|355490509|gb|AES71712.1| Serine/threonine protein
            kinase WNK8 [Medicago truncatula]
          Length = 614

 Score =  536 bits (1382), Expect = e-150
 Identities = 264/426 (61%), Positives = 324/426 (76%), Gaps = 18/426 (4%)
 Frame = -2

Query: 1241 DPAFVERSPDNRYIRYNKILGRGAFKTVYKGFDEIDGIEVAWSVVDMDDLLLSPENLERI 1062
            D   VE+ P  RY RY+++LG+GAFKTVYK FDE+DGIEVAW+ + ++D++ SP+NLE++
Sbjct: 7    DVGVVEKDPTLRYARYDEMLGKGAFKTVYKAFDEVDGIEVAWNQISIEDVMQSPQNLEKL 66

Query: 1061 YSEVHLLETLRHENVIKFYIFWEDNERKTINMITELFSSGSLRQYRKNHMNVDMKAIKNW 882
            YSEVHLL++L+HEN+IK Y  W D +   INMITELF+SGSLRQYRK H NVDMKAIKNW
Sbjct: 67   YSEVHLLKSLKHENIIKMYSSWVDEKSSNINMITELFTSGSLRQYRKKHKNVDMKAIKNW 126

Query: 881  AKQILLGLNYLHSQSPPIIHRDLKCDNIFVNGSHGEVKIGDLGFAKIMHQSTAHSVVGTP 702
            A+QIL GL +LHS +PP+IHRDLKCDNIFVNG++G+VKIGDLG A +M Q TA SV+GTP
Sbjct: 127  ARQILRGLCFLHSHNPPVIHRDLKCDNIFVNGNNGQVKIGDLGLAIVMQQPTARSVIGTP 186

Query: 701  EFMAPELYDEEYNELVDIYSFGLCILELITCEYPYKECKNQAQIYKKVTSGIKPASLGKI 522
            EFMAPELY+EEYNELVD+YSFG+CILE+ITCEYPY ECKN AQIYKKVTSGIKPASL ++
Sbjct: 187  EFMAPELYEEEYNELVDVYSFGMCILEMITCEYPYSECKNPAQIYKKVTSGIKPASLARV 246

Query: 521  NDPQAKQFIEKCIVAVSQRLSASELLKDPFLSS-----------------ESSISPQKLD 393
            NDP+ KQFIEKC+V  S RL ASELLKDPFL++                   S++P   +
Sbjct: 247  NDPEVKQFIEKCLVPASMRLPASELLKDPFLATGNTKEIYHDNLLLPNPPSKSLNPPTCE 306

Query: 392  PVNMVIDTNCDKLSSSTNAESSIETIHAWST-EFQRFNERNEYLLRGEKNDERTVSLSLR 216
            P  M ID+N    S ++  E   ET    S  +  R  E NE+ LRGEKN +RT+SL+LR
Sbjct: 307  PHPMEIDSNVKHTSPASTVERDKETSQVSSNHDILRKTENNEFRLRGEKNADRTISLTLR 366

Query: 215  ITDLRGQVRNIHFMFYLDADTTSSLAGEMVEQLDLWNEDVAFIAELIDDLIFSLVPTWKP 36
            I D  G  RNIHF FY+D+DTT S+A EMVE L+L +EDVA IAELI ++IF LVP WKP
Sbjct: 367  IADANGGARNIHFPFYIDSDTTISIAEEMVEHLELKDEDVAVIAELIHNMIFKLVPDWKP 426

Query: 35   SVGHNS 18
               ++S
Sbjct: 427  LCENSS 432