BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00002543
         (3739 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003549747.1| PREDICTED: topless-related protein 4-like [G...  1566   0.0  
ref|XP_002285341.2| PREDICTED: topless-related protein 4-like is...  1560   0.0  
ref|XP_003541476.1| PREDICTED: topless-related protein 4-like [G...  1554   0.0  
ref|XP_003633079.1| PREDICTED: topless-related protein 4-like [V...  1534   0.0  
ref|XP_004139298.1| PREDICTED: topless-related protein 4-like [C...  1533   0.0  

>ref|XP_003549747.1| PREDICTED: topless-related protein 4-like [Glycine max]
          Length = 1134

 Score = 1566 bits (4056), Expect = 0.0
 Identities = 757/986 (76%), Positives = 858/986 (87%), Gaps = 1/986 (0%)
 Frame = +2

Query: 431  HQLCKNPKPNPDIKTLFIDHSCGQSQPNGARAPSPVTNALMGGLPKPAGFPPLGAHGPFQ 610
            HQLCKNP+PNPDIKTLF+DHSCGQ QPNGARAPSPVTN LMG +PK  GFPPLGAHGPFQ
Sbjct: 183  HQLCKNPRPNPDIKTLFVDHSCGQVQPNGARAPSPVTNPLMGAVPKAGGFPPLGAHGPFQ 242

Query: 611  PAQGPLPTSLAGWMANPAPVAHPSASAGPIGFNSSNNAALLKRPRTPPSNNPAIDYQTAD 790
            P    LPTSLAGWMANP+PV HPSASAGPIG  ++NNAA+LKRPRTPPSNNPA+DYQTAD
Sbjct: 243  PTPAALPTSLAGWMANPSPVPHPSASAGPIGLAAANNAAILKRPRTPPSNNPAMDYQTAD 302

Query: 791  SEHVLKRSRPFGLSDDVNNPPVNILPVGYTSQNHGQSSFSTDDLPKAVVMTLSQGSAVKS 970
            S+HVLKR+RPFGLSD+V+N PVN+LPV Y+ Q+HGQSS+S+DDLPK VVMTL+QGS VKS
Sbjct: 303  SDHVLKRTRPFGLSDEVSNLPVNLLPVAYSGQSHGQSSYSSDDLPKTVVMTLNQGSIVKS 362

Query: 971  MDFHPVQQILLLVGTTSGEIMLWELGTREKLAQRNFKVWELGSCSMPLQASLANEYIASV 1150
            MDFHP+QQILLLVGT  G++M+W++G+RE++AQRNFKVWELG+CS+ LQASL+N+Y AS+
Sbjct: 363  MDFHPLQQILLLVGTNMGDVMVWDIGSRERIAQRNFKVWELGACSVALQASLSNDYSASI 422

Query: 1151 NRVIWSSDGTLFGVAYTKHIVHLYAYHGGDNLQDKLEIEAHVGSVNDLAFCYPNKQLCIV 1330
            NRV+WS DGTL  VAY+KHIVH+Y+YHGGD+L++ LEIEAH                   
Sbjct: 423  NRVVWSPDGTLCSVAYSKHIVHIYSYHGGDDLRNHLEIEAHA------------------ 464

Query: 1331 TCGEDRLIKAWEAHVGSVNDLAFCYPNKQLCIVTCGEDRLIKVWDANTGAKQYAFEGHEA 1510
                           GSVNDLAF YPNKQLC+VTCGEDR+IKVWDA TGAKQY FEGHEA
Sbjct: 465  ---------------GSVNDLAFSYPNKQLCVVTCGEDRVIKVWDAVTGAKQYTFEGHEA 509

Query: 1511 PVYSVCPHYKENIQFIFSTATDGKIKAWLYDNVGSRVDYDAPGHSSTALAYSADGTRLFS 1690
            PVYSVCPH+KE+IQFIFSTATDGKIKAWLYDN+GSRVDYDAPGHSST +AYSADGTRLFS
Sbjct: 510  PVYSVCPHHKESIQFIFSTATDGKIKAWLYDNMGSRVDYDAPGHSSTTMAYSADGTRLFS 569

Query: 1691 CGTNKEGDSYIVEWNESEGAVKRTYIGLGKRSVGVVQFDTIKNRFLAAGDECMVKFWDMD 1870
            CGTNKEG+S++VEWNESEGAVKRTY GLGKRSVGVVQFDT KNRFLAAGDE  +KFWDMD
Sbjct: 570  CGTNKEGESFLVEWNESEGAVKRTYHGLGKRSVGVVQFDTTKNRFLAAGDEFTIKFWDMD 629

Query: 1871 NVNVLTTTDAEGGLQASPCIRFNKEGILLAVSTNDNGIKILANTDGLRLLRTVESRSFDS 2050
            N N+LT+ +AEGGL ASPCIRFNK+GILLAVSTNDNG+KILAN +G+RLLRTVE+R+FD+
Sbjct: 630  NTNMLTSVEAEGGLLASPCIRFNKDGILLAVSTNDNGVKILANAEGIRLLRTVENRTFDA 689

Query: 2051 SRIASGAIVKPPALAAFGASNATVVANLVDRVAPVPSMVSLNGDSRNIPDVKPKITDESI 2230
            SR+AS A+VK P + AF ++N TV  +L DR  PV +MV +N D+RN+ DVKP+I DES+
Sbjct: 690  SRVASAAVVKAPTIGAFPSTNVTVGTSLADRAPPVAAMVGINNDTRNLADVKPRIVDESV 749

Query: 2231 EKSRVWKLTEINEPSQCRSLRLPDNISAMRVSRLIYTNSGVAILALAANAVHKLWKWQRN 2410
            EKSR+WKLTEINEPSQCRSL+LPD++S+MRVSRLIYTN GVAILALAANAVHKLWKWQRN
Sbjct: 750  EKSRIWKLTEINEPSQCRSLKLPDSLSSMRVSRLIYTNQGVAILALAANAVHKLWKWQRN 809

Query: 2411 ERNSSGKATASIVPQLWQPTSGILMTNDISDRNPEDAVPCFALSKNDSYVMSASGGKISL 2590
            ERN++GKATASI PQLWQP+SGILMTNDISD NPEDAV CFALSKNDSYVMSASGGKISL
Sbjct: 810  ERNTTGKATASIQPQLWQPSSGILMTNDISDTNPEDAVSCFALSKNDSYVMSASGGKISL 869

Query: 2591 FNXXXXXXXXXXXXXXXAASFLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGHHK 2770
            FN               AA+FLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGH K
Sbjct: 870  FNMMTFKTMTTFMPPPPAATFLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGHQK 929

Query: 2771 RITGLAFSNVLNVLISSGADSQLCVWSTDGWEKQTSRQLQIPSGRVAAPLADTRVQFHQD 2950
            RITGLAFS+VLNVL+SSGADSQLCVWSTDGWEKQ S+ LQ+PSGR  APLADTRVQFH D
Sbjct: 930  RITGLAFSHVLNVLVSSGADSQLCVWSTDGWEKQASKFLQMPSGRPPAPLADTRVQFHLD 989

Query: 2951 QTHLIAVHETQIAIYEAPKLECLKQWVPREASGPITHATYSCDSQSIYVSFEDGSVGVLT 3130
            QTHL+AVHETQIA+YEAPKLEC+KQ+ PREA+ PITHATYSCDSQSIYVSFEDGS+G+LT
Sbjct: 990  QTHLLAVHETQIALYEAPKLECIKQFSPREAN-PITHATYSCDSQSIYVSFEDGSIGILT 1048

Query: 3131 ATSLRLRCRINPASYLPANPSLRVYPLVIAAHPIEPTQFALGLSDGGVYILEPLESEGKW 3310
              +LRLRCRIN ++YL  NPSLRV+PLVIAAHP EP QFALGL+DGGV++LEPLE+EGKW
Sbjct: 1049 VPALRLRCRINQSAYLHPNPSLRVHPLVIAAHPSEPNQFALGLTDGGVHVLEPLEAEGKW 1108

Query: 3311 GTLPP-ENNAGPSATAGAANIDQPQR 3385
            GT PP EN AGPS  +GAA  +QPQR
Sbjct: 1109 GTPPPNENGAGPSTASGAAVSEQPQR 1134



 Score =  115 bits (287), Expect(2) = 3e-38
 Identities = 55/58 (94%), Positives = 57/58 (98%)
 Frame = +3

Query: 189 NEQLSKYGDTKSARGVMLAELKKLIEANPLFRDKLNFPTLRNSRLRTLINQSLNWQHQ 362
           NEQLSKYGDTKSARG+MLAELKKLIEANPLFRDKL FPTL+NSRLRTLINQSLNWQHQ
Sbjct: 127 NEQLSKYGDTKSARGIMLAELKKLIEANPLFRDKLQFPTLKNSRLRTLINQSLNWQHQ 184



 Score = 73.2 bits (178), Expect(2) = 3e-38
 Identities = 36/40 (90%), Positives = 40/40 (100%)
 Frame = +1

Query: 1   EALDKKDRAKAVDILVKDLKVFSAFNEDLFKEITQLLTLE 120
           EALDK+DRAKAVDILVKDLKVF+AFNE+LFKEITQLLTL+
Sbjct: 83  EALDKQDRAKAVDILVKDLKVFAAFNEELFKEITQLLTLD 122


>ref|XP_002285341.2| PREDICTED: topless-related protein 4-like isoform 1 [Vitis vinifera]
            gi|297738983|emb|CBI28228.3| unnamed protein product
            [Vitis vinifera]
          Length = 1133

 Score = 1560 bits (4038), Expect = 0.0
 Identities = 765/987 (77%), Positives = 851/987 (86%), Gaps = 2/987 (0%)
 Frame = +2

Query: 431  HQLCKNPKPNPDIKTLFIDHSCGQSQPNGARAPSPVTNALMGGLPKPAGFPPLGAHGPFQ 610
            HQLCKNPK NPDIKTLF+DH+CGQ  PNGARAPSPVTN LMG +PK  GFPPL AHGPFQ
Sbjct: 183  HQLCKNPKANPDIKTLFVDHTCGQ--PNGARAPSPVTNPLMGTVPKAGGFPPLSAHGPFQ 240

Query: 611  PAQGPLPTSLAGWMANPAPVAHPSASAGPIGFNSSNNAA-LLKRPRTPPSNNPAIDYQTA 787
            PA  PLPTSLAGWMANP+PV HPSASAGP+G  ++NNAA +LKRPRTPP+NNPA+DYQTA
Sbjct: 241  PAPAPLPTSLAGWMANPSPVPHPSASAGPMGLATANNAAAILKRPRTPPTNNPAMDYQTA 300

Query: 788  DSEHVLKRSRPFGLSDDVNNPPVNILPVGYTSQNHGQSSFSTDDLPKAVVMTLSQGSAVK 967
            DSEHVLKR RPFG+SD+VNN PVNILPV YT Q+HGQSS+S+DDLPK VVM+L QGS V+
Sbjct: 301  DSEHVLKRPRPFGISDEVNNLPVNILPVAYTGQSHGQSSYSSDDLPKTVVMSLPQGSTVR 360

Query: 968  SMDFHPVQQILLLVGTTSGEIMLWELGTREKLAQRNFKVWELGSCSMPLQASLANEYIAS 1147
            SMDFHPVQQILLLVGT  G+IM+W+LG+RE+LA +NFKVWEL SCSM LQ SLAN+Y+AS
Sbjct: 361  SMDFHPVQQILLLVGTNMGDIMVWDLGSRERLAIKNFKVWELASCSMALQTSLANDYLAS 420

Query: 1148 VNRVIWSSDGTLFGVAYTKHIVHLYAYHGGDNLQDKLEIEAHVGSVNDLAFCYPNKQLCI 1327
            VNRV+WS DGTLFGVAY+KHIVHLY+YH GD+L++ LEIEAH                  
Sbjct: 421  VNRVMWSPDGTLFGVAYSKHIVHLYSYHNGDDLRNHLEIEAH------------------ 462

Query: 1328 VTCGEDRLIKAWEAHVGSVNDLAFCYPNKQLCIVTCGEDRLIKVWDANTGAKQYAFEGHE 1507
                           VGSVNDLAF YPNK LC+VTCGEDR IKVWDANTG+KQY FEGHE
Sbjct: 463  ---------------VGSVNDLAFSYPNK-LCVVTCGEDRFIKVWDANTGSKQYTFEGHE 506

Query: 1508 APVYSVCPHYKENIQFIFSTATDGKIKAWLYDNVGSRVDYDAPGHSSTALAYSADGTRLF 1687
            APVYSVCPH+KENIQFIFSTA DGKIKAWLYDN+GSRVDYDAPGHSST +AYSADGTRLF
Sbjct: 507  APVYSVCPHHKENIQFIFSTAIDGKIKAWLYDNMGSRVDYDAPGHSSTTMAYSADGTRLF 566

Query: 1688 SCGTNKEGDSYIVEWNESEGAVKRTYIGLGKRSVGVVQFDTIKNRFLAAGDECMVKFWDM 1867
            SCGTNKEGDSYIVEWNESEGAVKRTY GLGKRSVGVVQFDT KNRFLAAGDE +VKFWDM
Sbjct: 567  SCGTNKEGDSYIVEWNESEGAVKRTYHGLGKRSVGVVQFDTTKNRFLAAGDEFLVKFWDM 626

Query: 1868 DNVNVLTTTDAEGGLQASPCIRFNKEGILLAVSTNDNGIKILANTDGLRLLRTVESRSFD 2047
            DNVN+L TTDAEGGL ASPCIRFNKEGILLAVSTN+NGIKILAN +G+RLLRT+E+RSFD
Sbjct: 627  DNVNLLMTTDAEGGLPASPCIRFNKEGILLAVSTNENGIKILANQEGIRLLRTMENRSFD 686

Query: 2048 SSRIASGAIVKPPALAAFGASNATVVANLVDRVAPVPSMVSLNGDSRNIPDVKPKITDES 2227
            +SR+AS A+VK PA+  F  +N  V  ++ DR APV +MV +N D+R++ DVKP+I DES
Sbjct: 687  ASRVASAAVVKAPAIGTFPPANPAVGTSIGDRAAPVAAMVGMNSDNRSLVDVKPRIADES 746

Query: 2228 IEKSRVWKLTEINEPSQCRSLRLPDNISAMRVSRLIYTNSGVAILALAANAVHKLWKWQR 2407
             EKSR+WKLTEINE SQCRSLRLPDN++AMRVSRL+YTNSG AILALA+NAVHKLWKWQR
Sbjct: 747  GEKSRIWKLTEINEQSQCRSLRLPDNLTAMRVSRLMYTNSGFAILALASNAVHKLWKWQR 806

Query: 2408 NERNSSGKATASIVPQLWQPTSGILMTNDISDRNPEDAVPCFALSKNDSYVMSASGGKIS 2587
            N+RN + KATAS+ PQLWQP+SGILMTN+ISD NPEDAVPCFALSKNDSYVMSASGGK+S
Sbjct: 807  NDRNITTKATASVAPQLWQPSSGILMTNEISDTNPEDAVPCFALSKNDSYVMSASGGKVS 866

Query: 2588 LFNXXXXXXXXXXXXXXXAASFLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGHH 2767
            LFN               AA+FLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGH 
Sbjct: 867  LFNMMTFKTMTTFMPPPPAATFLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGHQ 926

Query: 2768 KRITGLAFSNVLNVLISSGADSQLCVWSTDGWEKQTSRQLQIPSGRVAAPLADTRVQFHQ 2947
            KR+TGLAFS VLNVL+SSGADSQLCVW+TDGWEKQ S+ LQ+  G+ AAPLADTRVQFH 
Sbjct: 927  KRVTGLAFSPVLNVLVSSGADSQLCVWNTDGWEKQASKFLQVSPGQAAAPLADTRVQFHH 986

Query: 2948 DQTHLIAVHETQIAIYEAPKLECLKQWVPREASGPITHATYSCDSQSIYVSFEDGSVGVL 3127
            DQ HL+AVHETQIAI+EA KLECL+QWVPREASG ITHATYSCDSQSI+VSFEDGSVGVL
Sbjct: 987  DQIHLLAVHETQIAIFEASKLECLRQWVPREASGSITHATYSCDSQSIFVSFEDGSVGVL 1046

Query: 3128 TATSLRLRCRINPASYLPANPSLRVYPLVIAAHPIEPTQFALGLSDGGVYILEPLESEGK 3307
            TA++LR RCRINP +YLP NPSLRVYPLV+AAHP EP QFALGL+DGGV +LEPLESEGK
Sbjct: 1047 TASTLRSRCRINPTAYLPPNPSLRVYPLVVAAHPSEPNQFALGLTDGGVCVLEPLESEGK 1106

Query: 3308 WGTLPP-ENNAGPSATAGAANIDQPQR 3385
            WGT PP EN AGPS+T+GAA  DQPQR
Sbjct: 1107 WGTSPPLENGAGPSSTSGAAGSDQPQR 1133



 Score =  113 bits (283), Expect(2) = 4e-38
 Identities = 54/58 (93%), Positives = 57/58 (98%)
 Frame = +3

Query: 189 NEQLSKYGDTKSARGVMLAELKKLIEANPLFRDKLNFPTLRNSRLRTLINQSLNWQHQ 362
           NEQLSKYGDTKSARG+MLAELKKLIEANPLFRDKL FP+L+NSRLRTLINQSLNWQHQ
Sbjct: 127 NEQLSKYGDTKSARGIMLAELKKLIEANPLFRDKLQFPSLKNSRLRTLINQSLNWQHQ 184



 Score = 74.3 bits (181), Expect(2) = 4e-38
 Identities = 37/40 (92%), Positives = 40/40 (100%)
 Frame = +1

Query: 1   EALDKKDRAKAVDILVKDLKVFSAFNEDLFKEITQLLTLE 120
           EALDK+DRAKAV+ILVKDLKVFSAFNE+LFKEITQLLTLE
Sbjct: 83  EALDKRDRAKAVEILVKDLKVFSAFNEELFKEITQLLTLE 122


>ref|XP_003541476.1| PREDICTED: topless-related protein 4-like [Glycine max]
          Length = 1232

 Score = 1554 bits (4024), Expect = 0.0
 Identities = 753/986 (76%), Positives = 857/986 (86%), Gaps = 1/986 (0%)
 Frame = +2

Query: 431  HQLCKNPKPNPDIKTLFIDHSCGQSQPNGARAPSPVTNALMGGLPKPAGFPPLGAHGPFQ 610
            HQLCKNP+PNPDIKTLF+DHSCGQ  PNGARAPSPVTN LMG +PK  GFPPLGAHGPFQ
Sbjct: 283  HQLCKNPRPNPDIKTLFVDHSCGQ--PNGARAPSPVTNPLMGAVPKAGGFPPLGAHGPFQ 340

Query: 611  PAQGPLPTSLAGWMANPAPVAHPSASAGPIGFNSSNNAALLKRPRTPPSNNPAIDYQTAD 790
            P    LPTSLAGWMANP+PV HPSASAGPIG  ++NNAA+LKRPRTPP+NNPA+DYQTAD
Sbjct: 341  PTPAALPTSLAGWMANPSPVPHPSASAGPIGLAAANNAAILKRPRTPPTNNPAMDYQTAD 400

Query: 791  SEHVLKRSRPFGLSDDVNNPPVNILPVGYTSQNHGQSSFSTDDLPKAVVMTLSQGSAVKS 970
            S+HVLKR+RPFGLSD+V+N PVN+LPV Y+ Q+HGQSS+S+DDLPK +VMTL+QGS VKS
Sbjct: 401  SDHVLKRTRPFGLSDEVSNLPVNLLPVAYSGQSHGQSSYSSDDLPKTIVMTLNQGSIVKS 460

Query: 971  MDFHPVQQILLLVGTTSGEIMLWELGTREKLAQRNFKVWELGSCSMPLQASLANEYIASV 1150
            MDFHP+QQILLLVGT  G++M+W++G+RE++AQRNFKVWELGSCS+ LQASL+N+Y ASV
Sbjct: 461  MDFHPLQQILLLVGTNMGDVMVWDIGSRERIAQRNFKVWELGSCSVALQASLSNDYSASV 520

Query: 1151 NRVIWSSDGTLFGVAYTKHIVHLYAYHGGDNLQDKLEIEAHVGSVNDLAFCYPNKQLCIV 1330
            NRV+WS DGTL  VAY+KHIVH+Y+Y GGD+L++ LEIEAH                   
Sbjct: 521  NRVVWSPDGTLCSVAYSKHIVHIYSYQGGDDLRNHLEIEAHA------------------ 562

Query: 1331 TCGEDRLIKAWEAHVGSVNDLAFCYPNKQLCIVTCGEDRLIKVWDANTGAKQYAFEGHEA 1510
                           GSVNDLAF YPNKQLC+VTCGEDR+IKVWDA TGAKQY FEGHEA
Sbjct: 563  ---------------GSVNDLAFSYPNKQLCVVTCGEDRVIKVWDAVTGAKQYTFEGHEA 607

Query: 1511 PVYSVCPHYKENIQFIFSTATDGKIKAWLYDNVGSRVDYDAPGHSSTALAYSADGTRLFS 1690
            PVYSVCPH+KE+IQFIFSTATDGKIKAWLYDN+GSRVDYDAPGHSST +AYSADGTRLFS
Sbjct: 608  PVYSVCPHHKESIQFIFSTATDGKIKAWLYDNMGSRVDYDAPGHSSTTMAYSADGTRLFS 667

Query: 1691 CGTNKEGDSYIVEWNESEGAVKRTYIGLGKRSVGVVQFDTIKNRFLAAGDECMVKFWDMD 1870
            CGTNKEG+S++VEWNESEGAVKRTY GLGKRSVGVVQFDT KNRFLAAGDE M+KFWDMD
Sbjct: 668  CGTNKEGESFLVEWNESEGAVKRTYHGLGKRSVGVVQFDTTKNRFLAAGDEFMIKFWDMD 727

Query: 1871 NVNVLTTTDAEGGLQASPCIRFNKEGILLAVSTNDNGIKILANTDGLRLLRTVESRSFDS 2050
            N N+LT+ +A+GGL ASPCIRFNK+GILLAVSTND+G+KILAN +G+RLLRTVE+R+FD+
Sbjct: 728  NTNMLTSVEADGGLLASPCIRFNKDGILLAVSTNDSGVKILANAEGIRLLRTVENRTFDA 787

Query: 2051 SRIASGAIVKPPALAAFGASNATVVANLVDRVAPVPSMVSLNGDSRNIPDVKPKITDESI 2230
            SR+AS A+VK P + AF ++N TV  +L DR  PV +MV +N D+RN+ DVKP+I DE++
Sbjct: 788  SRVASAAVVKAPTIGAFPSTNVTVGTSLADRAPPVAAMVGINNDTRNLADVKPRIVDEAV 847

Query: 2231 EKSRVWKLTEINEPSQCRSLRLPDNISAMRVSRLIYTNSGVAILALAANAVHKLWKWQRN 2410
            EKSR+WKLTEINEPSQCRSL+LPD++S+MRVSRLIYTN GVAILALAANAVHKLWKWQRN
Sbjct: 848  EKSRIWKLTEINEPSQCRSLKLPDSLSSMRVSRLIYTNQGVAILALAANAVHKLWKWQRN 907

Query: 2411 ERNSSGKATASIVPQLWQPTSGILMTNDISDRNPEDAVPCFALSKNDSYVMSASGGKISL 2590
            ERN++GKATASI PQLWQP+SGILMTNDISD NPEDAV CFALSKNDSYVMSASGGKISL
Sbjct: 908  ERNTTGKATASIQPQLWQPSSGILMTNDISDTNPEDAVSCFALSKNDSYVMSASGGKISL 967

Query: 2591 FNXXXXXXXXXXXXXXXAASFLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGHHK 2770
            FN               AA+FLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGH K
Sbjct: 968  FNMMTFKTMTTFMPPPPAATFLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGHQK 1027

Query: 2771 RITGLAFSNVLNVLISSGADSQLCVWSTDGWEKQTSRQLQIPSGRVAAPLADTRVQFHQD 2950
            RITGLAFS+VLNVL+SSGADSQLCVWSTDGWEKQ S+ LQ+PSGR  APLADTRVQFH D
Sbjct: 1028 RITGLAFSHVLNVLVSSGADSQLCVWSTDGWEKQASKFLQMPSGRPPAPLADTRVQFHLD 1087

Query: 2951 QTHLIAVHETQIAIYEAPKLECLKQWVPREASGPITHATYSCDSQSIYVSFEDGSVGVLT 3130
            QTHL+AVHETQIA+YEAPKLEC+KQ+ PREA+ PITHATYSCDSQSIYVSFEDGS+G+LT
Sbjct: 1088 QTHLLAVHETQIALYEAPKLECIKQFSPREAN-PITHATYSCDSQSIYVSFEDGSIGILT 1146

Query: 3131 ATSLRLRCRINPASYLPANPSLRVYPLVIAAHPIEPTQFALGLSDGGVYILEPLESEGKW 3310
              +LRLRCRIN ++YL  NPSLRV+PLVIAAHP EP QFALGL+DGGV++LEPLE+EGKW
Sbjct: 1147 VPALRLRCRINQSAYLHPNPSLRVHPLVIAAHPSEPNQFALGLTDGGVHVLEPLEAEGKW 1206

Query: 3311 GTLPP-ENNAGPSATAGAANIDQPQR 3385
            GT PP EN AGPS T+GAA  +Q QR
Sbjct: 1207 GTPPPNENGAGPSTTSGAAVSEQTQR 1232



 Score =  115 bits (287), Expect(2) = 3e-38
 Identities = 55/58 (94%), Positives = 57/58 (98%)
 Frame = +3

Query: 189 NEQLSKYGDTKSARGVMLAELKKLIEANPLFRDKLNFPTLRNSRLRTLINQSLNWQHQ 362
           NEQLSKYGDTKSARG+MLAELKKLIEANPLFRDKL FPTL+NSRLRTLINQSLNWQHQ
Sbjct: 227 NEQLSKYGDTKSARGIMLAELKKLIEANPLFRDKLQFPTLKNSRLRTLINQSLNWQHQ 284



 Score = 73.2 bits (178), Expect(2) = 3e-38
 Identities = 36/40 (90%), Positives = 40/40 (100%)
 Frame = +1

Query: 1   EALDKKDRAKAVDILVKDLKVFSAFNEDLFKEITQLLTLE 120
           EALDK+DRAKAVDILVKDLKVF+AFNE+LFKEITQLLTL+
Sbjct: 183 EALDKQDRAKAVDILVKDLKVFAAFNEELFKEITQLLTLD 222


>ref|XP_003633079.1| PREDICTED: topless-related protein 4-like [Vitis vinifera]
          Length = 1123

 Score = 1534 bits (3972), Expect = 0.0
 Identities = 756/987 (76%), Positives = 842/987 (85%), Gaps = 2/987 (0%)
 Frame = +2

Query: 431  HQLCKNPKPNPDIKTLFIDHSCGQSQPNGARAPSPVTNALMGGLPKPAGFPPLGAHGPFQ 610
            HQLCKNPK NPDIKTLF+DH+CGQ  PNGARAPSPVTN LMG +PK  GFPPL AHGPFQ
Sbjct: 183  HQLCKNPKANPDIKTLFVDHTCGQ--PNGARAPSPVTNPLMGTVPKAGGFPPLSAHGPFQ 240

Query: 611  PAQGPLPTSLAGWMANPAPVAHPSASAGPIGFNSSNNAA-LLKRPRTPPSNNPAIDYQTA 787
            PA  PLPTSLAGWMANP+PV HPSASAGP+G  ++NNAA +LKRPRTPP+NNPA+DYQTA
Sbjct: 241  PAPAPLPTSLAGWMANPSPVPHPSASAGPMGLATANNAAAILKRPRTPPTNNPAMDYQTA 300

Query: 788  DSEHVLKRSRPFGLSDDVNNPPVNILPVGYTSQNHGQSSFSTDDLPKAVVMTLSQGSAVK 967
            DSEHVLKR RPFG+SD+V           YT Q+HGQSS+S+DDLPK VVM+L QGS V+
Sbjct: 301  DSEHVLKRPRPFGISDEV----------AYTGQSHGQSSYSSDDLPKTVVMSLPQGSTVR 350

Query: 968  SMDFHPVQQILLLVGTTSGEIMLWELGTREKLAQRNFKVWELGSCSMPLQASLANEYIAS 1147
            SMDFHPVQQILLLVGT  G+IM+W+LG+RE+LA +NFKVWEL SCSM LQ SLAN+Y+AS
Sbjct: 351  SMDFHPVQQILLLVGTNMGDIMVWDLGSRERLAIKNFKVWELASCSMALQTSLANDYLAS 410

Query: 1148 VNRVIWSSDGTLFGVAYTKHIVHLYAYHGGDNLQDKLEIEAHVGSVNDLAFCYPNKQLCI 1327
            VNRV+WS DGTLFGVAY+KHIVHLY+YH GD+L++ LEIEAH                  
Sbjct: 411  VNRVMWSPDGTLFGVAYSKHIVHLYSYHNGDDLRNHLEIEAH------------------ 452

Query: 1328 VTCGEDRLIKAWEAHVGSVNDLAFCYPNKQLCIVTCGEDRLIKVWDANTGAKQYAFEGHE 1507
                           VGSVNDLAF YPNK LC+VTCGEDR IKVWDANTG+KQY FEGHE
Sbjct: 453  ---------------VGSVNDLAFSYPNK-LCVVTCGEDRFIKVWDANTGSKQYTFEGHE 496

Query: 1508 APVYSVCPHYKENIQFIFSTATDGKIKAWLYDNVGSRVDYDAPGHSSTALAYSADGTRLF 1687
            APVYSVCPH+KENIQFIFSTA DGKIKAWLYDN+GSRVDYDAPGHSST +AYSADGTRLF
Sbjct: 497  APVYSVCPHHKENIQFIFSTAIDGKIKAWLYDNMGSRVDYDAPGHSSTTMAYSADGTRLF 556

Query: 1688 SCGTNKEGDSYIVEWNESEGAVKRTYIGLGKRSVGVVQFDTIKNRFLAAGDECMVKFWDM 1867
            SCGTNKEGDSYIVEWNESEGAVKRTY GLGKRSVGVVQFDT KNRFLAAGDE +VKFWDM
Sbjct: 557  SCGTNKEGDSYIVEWNESEGAVKRTYHGLGKRSVGVVQFDTTKNRFLAAGDEFLVKFWDM 616

Query: 1868 DNVNVLTTTDAEGGLQASPCIRFNKEGILLAVSTNDNGIKILANTDGLRLLRTVESRSFD 2047
            DNVN+L TTDAEGGL ASPCIRFNKEGILLAVSTN+NGIKILAN +G+RLLRT+E+RSFD
Sbjct: 617  DNVNLLMTTDAEGGLPASPCIRFNKEGILLAVSTNENGIKILANQEGIRLLRTMENRSFD 676

Query: 2048 SSRIASGAIVKPPALAAFGASNATVVANLVDRVAPVPSMVSLNGDSRNIPDVKPKITDES 2227
            +SR+AS A+VK PA+  F  +N  V  ++ DR APV +MV +N D+R++ DVKP+I DES
Sbjct: 677  ASRVASAAVVKAPAIGTFPPANPAVGTSIGDRAAPVAAMVGMNSDNRSLVDVKPRIADES 736

Query: 2228 IEKSRVWKLTEINEPSQCRSLRLPDNISAMRVSRLIYTNSGVAILALAANAVHKLWKWQR 2407
             EKSR+WKLTEINE SQCRSLRLPDN++AMRVSRL+YTNSG AILALA+NAVHKLWKWQR
Sbjct: 737  GEKSRIWKLTEINEQSQCRSLRLPDNLTAMRVSRLMYTNSGFAILALASNAVHKLWKWQR 796

Query: 2408 NERNSSGKATASIVPQLWQPTSGILMTNDISDRNPEDAVPCFALSKNDSYVMSASGGKIS 2587
            N+RN + KATAS+ PQLWQP+SGILMTN+ISD NPEDAVPCFALSKNDSYVMSASGGK+S
Sbjct: 797  NDRNITTKATASVAPQLWQPSSGILMTNEISDTNPEDAVPCFALSKNDSYVMSASGGKVS 856

Query: 2588 LFNXXXXXXXXXXXXXXXAASFLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGHH 2767
            LFN               AA+FLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGH 
Sbjct: 857  LFNMMTFKTMTTFMPPPPAATFLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGHQ 916

Query: 2768 KRITGLAFSNVLNVLISSGADSQLCVWSTDGWEKQTSRQLQIPSGRVAAPLADTRVQFHQ 2947
            KR+TGLAFS VLNVL+SSGADSQLCVW+TDGWEKQ S+ LQ+  G+ AAPLADTRVQFH 
Sbjct: 917  KRVTGLAFSPVLNVLVSSGADSQLCVWNTDGWEKQASKFLQVSPGQAAAPLADTRVQFHH 976

Query: 2948 DQTHLIAVHETQIAIYEAPKLECLKQWVPREASGPITHATYSCDSQSIYVSFEDGSVGVL 3127
            DQ HL+AVHETQIAI+EA KLECL+QWVPREASG ITHATYSCDSQSI+VSFEDGSVGVL
Sbjct: 977  DQIHLLAVHETQIAIFEASKLECLRQWVPREASGSITHATYSCDSQSIFVSFEDGSVGVL 1036

Query: 3128 TATSLRLRCRINPASYLPANPSLRVYPLVIAAHPIEPTQFALGLSDGGVYILEPLESEGK 3307
            TA++LR RCRINP +YLP NPSLRVYPLV+AAHP EP QFALGL+DGGV +LEPLESEGK
Sbjct: 1037 TASTLRSRCRINPTAYLPPNPSLRVYPLVVAAHPSEPNQFALGLTDGGVCVLEPLESEGK 1096

Query: 3308 WGTLPP-ENNAGPSATAGAANIDQPQR 3385
            WGT PP EN AGPS+T+GAA  DQPQR
Sbjct: 1097 WGTSPPLENGAGPSSTSGAAGSDQPQR 1123



 Score =  113 bits (283), Expect(2) = 4e-38
 Identities = 54/58 (93%), Positives = 57/58 (98%)
 Frame = +3

Query: 189 NEQLSKYGDTKSARGVMLAELKKLIEANPLFRDKLNFPTLRNSRLRTLINQSLNWQHQ 362
           NEQLSKYGDTKSARG+MLAELKKLIEANPLFRDKL FP+L+NSRLRTLINQSLNWQHQ
Sbjct: 127 NEQLSKYGDTKSARGIMLAELKKLIEANPLFRDKLQFPSLKNSRLRTLINQSLNWQHQ 184



 Score = 74.3 bits (181), Expect(2) = 4e-38
 Identities = 37/40 (92%), Positives = 40/40 (100%)
 Frame = +1

Query: 1   EALDKKDRAKAVDILVKDLKVFSAFNEDLFKEITQLLTLE 120
           EALDK+DRAKAV+ILVKDLKVFSAFNE+LFKEITQLLTLE
Sbjct: 83  EALDKRDRAKAVEILVKDLKVFSAFNEELFKEITQLLTLE 122


>ref|XP_004139298.1| PREDICTED: topless-related protein 4-like [Cucumis sativus]
          Length = 1134

 Score = 1533 bits (3968), Expect = 0.0
 Identities = 746/987 (75%), Positives = 841/987 (85%), Gaps = 2/987 (0%)
 Frame = +2

Query: 431  HQLCKNPKPNPDIKTLFIDHSCGQSQPNGARAPSPVTNALMGGLPKPAGFPPLGAHGPFQ 610
            HQLCKNP+PNPDIKTLF+DHSCGQ  PNGARAPSPVTN LMGG+PK A FPPL AHGPFQ
Sbjct: 183  HQLCKNPRPNPDIKTLFVDHSCGQ--PNGARAPSPVTNPLMGGVPKAAAFPPLSAHGPFQ 240

Query: 611  PAQGPLPTSLAGWMANPAPVAHPSASAGPIGFNSSNNAALLKRPRTPPSNNPAIDYQTAD 790
            P    LPTSLAGWMANP+PV HPSASA PIG N++NNAA+LKRPRTPP+NNP +DYQTAD
Sbjct: 241  PTPAALPTSLAGWMANPSPVPHPSASAAPIGLNAANNAAILKRPRTPPTNNPTMDYQTAD 300

Query: 791  SEHVLKRSRPFGLSDDVNNPPVNILPVGYTSQNHGQSSFSTDDLPKAVVMTLSQGSAVKS 970
            SEHVLKRSRPFGLS++V N PVNILPVGY +Q HGQSS+S+DDLPK VV TLSQGS VKS
Sbjct: 301  SEHVLKRSRPFGLSEEVTNLPVNILPVGYGNQGHGQSSYSSDDLPKNVVTTLSQGSVVKS 360

Query: 971  MDFHPVQQILLLVGTTSGEIMLWELGTREKLAQRNFKVWELGSCSMPLQASLANEYIASV 1150
            MDFHP QQ +LLVGT  G++M+WE+G RE++A RNFKVW+L + S+ LQASLA++Y AS+
Sbjct: 361  MDFHPQQQTILLVGTNVGDVMIWEVGGRERIAIRNFKVWDLAARSVALQASLASDYTASI 420

Query: 1151 NRVIWSSDGTLFGVAYTKHIVHLYAYHGGDNLQDKLEIEAHVGSVNDLAFCYPNKQLCIV 1330
            NRV+WS DGTLFGVAY+KHIVH+Y+Y  GD L++ LEIEAH                   
Sbjct: 421  NRVMWSPDGTLFGVAYSKHIVHIYSYQAGDELRNHLEIEAH------------------- 461

Query: 1331 TCGEDRLIKAWEAHVGSVNDLAFCYPNKQLCIVTCGEDRLIKVWDANTGAKQYAFEGHEA 1510
                          VGSVNDLAF Y NKQLC+VTCGEDR+IKVWDA TG KQ+ FEGH+A
Sbjct: 462  --------------VGSVNDLAFLYANKQLCLVTCGEDRVIKVWDAVTGVKQFTFEGHDA 507

Query: 1511 PVYSVCPHYKENIQFIFSTATDGKIKAWLYDNVGSRVDYDAPGHSSTALAYSADGTRLFS 1690
            PVYS+CPH+KENIQFIFSTA DGKIKAWLYDN+GSRVDYDAPGHSST +AYSADGTRLFS
Sbjct: 508  PVYSICPHHKENIQFIFSTAADGKIKAWLYDNMGSRVDYDAPGHSSTTMAYSADGTRLFS 567

Query: 1691 CGTNKEGDSYIVEWNESEGAVKRTYIGLGKRSVGVVQFDTIKNRFLAAGDECMVKFWDMD 1870
            CGTNK+G+SY+VEWNESEGAVKRTY GLGKRSVGVVQFDT KNRFLAAGD+  VKFWDMD
Sbjct: 568  CGTNKDGESYLVEWNESEGAVKRTYQGLGKRSVGVVQFDTTKNRFLAAGDDFSVKFWDMD 627

Query: 1871 NVNVLTTTDAEGGLQASPCIRFNKEGILLAVSTNDNGIKILANTDGLRLLRTVESRSFDS 2050
            +VN+LT+ DA+GGL ASPCIRFNK+G+LLAVSTNDNGIKILAN +G R+LRTVE+R+FD+
Sbjct: 628  SVNILTSIDADGGLPASPCIRFNKDGVLLAVSTNDNGIKILANAEGFRMLRTVENRTFDA 687

Query: 2051 SRIASGAIVKPPALAAFGASNATVVANLVDRVAPVPS-MVSLNGDSRNIPDVKPKITDES 2227
            SR+AS A+VK P + +FG    +V  ++ DR  PV + MV +N DSR++ DVKP+I DES
Sbjct: 688  SRVASAAVVKAPPIGSFGPPAVSVGMSIGDRTPPVAAAMVGINNDSRSLADVKPRIADES 747

Query: 2228 IEKSRVWKLTEINEPSQCRSLRLPDNISAMRVSRLIYTNSGVAILALAANAVHKLWKWQR 2407
            ++KSR+WKLTEINEP+QCRSLRLPDN++A RVSRLIYTNSG+AILALA+NAVHKLW+WQR
Sbjct: 748  VDKSRIWKLTEINEPTQCRSLRLPDNLTASRVSRLIYTNSGLAILALASNAVHKLWRWQR 807

Query: 2408 NERNSSGKATASIVPQLWQPTSGILMTNDISDRNPEDAVPCFALSKNDSYVMSASGGKIS 2587
            N+RN + KATAS+ PQLWQP SGILMTNDISD NPEDAVPCFALSKNDSYVMSASGGKIS
Sbjct: 808  NDRNVTVKATASVAPQLWQPPSGILMTNDISDTNPEDAVPCFALSKNDSYVMSASGGKIS 867

Query: 2588 LFNXXXXXXXXXXXXXXXAASFLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGHH 2767
            LFN               AA+FLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGH 
Sbjct: 868  LFNMMTFKTMTTFMPPPPAATFLAFHPQDNNIIAIGMEDSSIQIYNVRVDEVKTKLKGHQ 927

Query: 2768 KRITGLAFSNVLNVLISSGADSQLCVWSTDGWEKQTSRQLQIPSGRVAAPLADTRVQFHQ 2947
            KRITGLAFSN LNVL+SSGADSQLCVWSTDGWEKQ ++ LQ+PS R  APLADTRVQFH 
Sbjct: 928  KRITGLAFSNQLNVLVSSGADSQLCVWSTDGWEKQVNKFLQVPSSRTTAPLADTRVQFHI 987

Query: 2948 DQTHLIAVHETQIAIYEAPKLECLKQWVPREASGPITHATYSCDSQSIYVSFEDGSVGVL 3127
            DQ HL+A+HETQIAIYEAPKLECLKQWVPREASGPITHAT+SCDSQSIYVSFEDGSVGVL
Sbjct: 988  DQIHLLAIHETQIAIYEAPKLECLKQWVPREASGPITHATFSCDSQSIYVSFEDGSVGVL 1047

Query: 3128 TATSLRLRCRINPASYLPANPSLRVYPLVIAAHPIEPTQFALGLSDGGVYILEPLESEGK 3307
            TA++LRLRCRINP +YL +NPSLRV+PLVIAAHP EP QFALGLSDGGV++LEP ESEGK
Sbjct: 1048 TASTLRLRCRINPNAYLSSNPSLRVHPLVIAAHPSEPNQFALGLSDGGVHVLEPSESEGK 1107

Query: 3308 WGTLPP-ENNAGPSATAGAANIDQPQR 3385
            WGT PP EN AGPS   GAA  DQPQR
Sbjct: 1108 WGTSPPVENGAGPSTATGAAGPDQPQR 1134



 Score =  115 bits (287), Expect(2) = 5e-39
 Identities = 55/58 (94%), Positives = 57/58 (98%)
 Frame = +3

Query: 189 NEQLSKYGDTKSARGVMLAELKKLIEANPLFRDKLNFPTLRNSRLRTLINQSLNWQHQ 362
           NEQLSKYGDTKSARG+MLAELKKLIEANPLFRDKL FPTL+NSRLRTLINQSLNWQHQ
Sbjct: 127 NEQLSKYGDTKSARGIMLAELKKLIEANPLFRDKLQFPTLKNSRLRTLINQSLNWQHQ 184



 Score = 75.9 bits (185), Expect(2) = 5e-39
 Identities = 38/40 (95%), Positives = 40/40 (100%)
 Frame = +1

Query: 1   EALDKKDRAKAVDILVKDLKVFSAFNEDLFKEITQLLTLE 120
           EALDK+DRAKAVDILVKDLKVFSAFNE+LFKEITQLLTLE
Sbjct: 83  EALDKRDRAKAVDILVKDLKVFSAFNEELFKEITQLLTLE 122