BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00001744
         (1926 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002533700.1| amsh, putative [Ricinus communis] gi|2235263...   640   0.0  
ref|XP_002278560.1| PREDICTED: AMSH-like ubiquitin thiolesterase...   634   e-179
ref|XP_003528952.1| PREDICTED: AMSH-like ubiquitin thiolesterase...   622   e-175
ref|XP_003534164.1| PREDICTED: AMSH-like ubiquitin thiolesterase...   619   e-175
ref|XP_003630863.1| AMSH-like protease [Medicago truncatula] gi|...   619   e-175

>ref|XP_002533700.1| amsh, putative [Ricinus communis] gi|223526395|gb|EEF28683.1| amsh,
            putative [Ricinus communis]
          Length = 514

 Score =  640 bits (1650), Expect = 0.0
 Identities = 339/539 (62%), Positives = 394/539 (73%), Gaps = 21/539 (3%)
 Frame = -1

Query: 1821 RINVAASTRTLDVDNRISLRYYFRIADNILKQANIFRAEKNYIDLYIMLLRFSSLVTETI 1642
            RIN+A S + +DVDNRISLR+Y+RIADNILKQA+IFR EKN IDLYIMLLRFSSLV+ETI
Sbjct: 9    RINIATSAQKIDVDNRISLRFYYRIADNILKQADIFREEKNIIDLYIMLLRFSSLVSETI 68

Query: 1641 PRHRDYRASSQREKNYLKKRSLDAVIELENIKPTVERMIDELSKKHTHSIKGREITNSN- 1465
            P HRDYR S Q +K YLK++ L+AV ELE +KP V++ I+EL++KHTH + G    N N 
Sbjct: 69   PCHRDYRTSQQSKKIYLKQKLLNAVKELEQLKPAVQQKINELNRKHTHQVNGWGSVNQND 128

Query: 1464 -------------------VKAQRPAVPEYV-HKNQIQQFSHTSSVEPQFRRLSLSIPLP 1345
                                KA  PA  E+V H ++ QQ S+   V  QFR++SL+ P P
Sbjct: 129  SLEWPPVKKKTLTGYDAGATKAVIPAAREFVYHGSRTQQLSYARPVGEQFRKMSLNFPQP 188

Query: 1344 KEETLSRHSILGPNGLRGQWQPPTSFKAISYPSDIDFTPVEIPSDLPRDKSQHHLVENGS 1165
            KEETLSRHSILGPNGL+GQWQPP S K + YPS+ID +PVEIP     D S    +ENG 
Sbjct: 189  KEETLSRHSILGPNGLQGQWQPPKSDKGVWYPSNIDLSPVEIPRF---DSS----LENGL 241

Query: 1164 LLKKDSISISENLPEKDSSITSDLESVLLPNNDDKMPQNEELGSLMPPKNDKKISLAEEL 985
             LK DS S             S+LE++                S++   ++ + S  EEL
Sbjct: 242  ALKLDSSS-------------SELETL-------------SSQSVLTVNDNSQTSRVEEL 275

Query: 984  GSLISFDTIETPAPMNLIRQPSPPPVLADVQDLIPTTSPPVTEAEYGRDDPSSNGFSCSE 805
              +ISF+T ETP   +LIRQPSPP VLA+VQDLIP  SP  TEAE   D  S +    SE
Sbjct: 276  SPMISFETTETPVQTDLIRQPSPPAVLAEVQDLIPAMSPQATEAENKMDISSPDDIVRSE 335

Query: 804  SPMELHISTTMMDRFMKLAKSNTNKNLETCGILAGSLKNRKFYITALIIPKQESTSDSCQ 625
            SP++LHISTTMM+ FMKLAK+NT++NLETCG+LAGSLKNRKFY+TALIIPKQESTSDSCQ
Sbjct: 336  SPLQLHISTTMMENFMKLAKANTDRNLETCGVLAGSLKNRKFYVTALIIPKQESTSDSCQ 395

Query: 624  ATNEEEIFDVQDKRSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPESVAIVMAPRDT 445
             TNEEEIF+VQDKRSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPESVAIVMAPRDT
Sbjct: 396  TTNEEEIFEVQDKRSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPESVAIVMAPRDT 455

Query: 444  SRNHGIFRLTTPGGMGVIRNCPHRGFHSHDPPSDGSPIYKQCTDVYLNPDVKFDVIDLR 268
            SR HGIFRLTTPGGM VIRNC  RGFH HD P DG PIYK CTDVY+NP++KFDVIDLR
Sbjct: 456  SRTHGIFRLTTPGGMSVIRNCQQRGFHPHDQPPDGGPIYKTCTDVYMNPNLKFDVIDLR 514


>ref|XP_002278560.1| PREDICTED: AMSH-like ubiquitin thiolesterase 1 [Vitis vinifera]
            gi|297734223|emb|CBI15470.3| unnamed protein product
            [Vitis vinifera]
          Length = 506

 Score =  634 bits (1634), Expect = e-179
 Identities = 335/540 (62%), Positives = 395/540 (73%), Gaps = 16/540 (2%)
 Frame = -1

Query: 1839 MKPYSVRINVAASTRTLDVDNRISLRYYFRIADNILKQANIFRAEKNYIDLYIMLLRFSS 1660
            M+P S  IN+AAS + LDVDNRISLRYY+RIADNILKQA+IFR EKN IDLYIMLLRFSS
Sbjct: 1    MRPSSGGINIAASAQKLDVDNRISLRYYYRIADNILKQADIFREEKNIIDLYIMLLRFSS 60

Query: 1659 LVTETIPRHRDYRASSQREKNYLKKRSLDAVIELENIKPTVERMIDELSKKHTHSI---- 1492
            L +ETIP HRDY+AS Q +KNYLKK  L A+ ELE +KP V + IDEL++KHT+ +    
Sbjct: 61   LTSETIPCHRDYKASLQSKKNYLKKNLLRALCELEELKPAVRQKIDELNQKHTYQVNRWG 120

Query: 1491 -----------KGREITNSNVKAQRPAVPEYVHK-NQIQQFSHTSSVEPQFRRLSLSIPL 1348
                       K     +   K  RP   ++ ++ ++IQQFS    VE QFRRLS+ I  
Sbjct: 121  ADSSLEWPHVRKQTLTKHEMTKTPRPTARDFEYQGSKIQQFSRAKPVEEQFRRLSVGILR 180

Query: 1347 PKEETLSRHSILGPNGLRGQWQPPTSFKAISYPSDIDFTPVEIPSDLPRDKSQHHLVENG 1168
            P+EETLSRHSILGPNGL GQWQPPT  K + YPS++D TPV+ PS       QHH VE+G
Sbjct: 181  PREETLSRHSILGPNGLHGQWQPPTIDKRVQYPSNLDLTPVQFPS------LQHH-VEDG 233

Query: 1167 SLLKKDSISISENLPEKDSSITSDLESVLLPNNDDKMPQNEELGSLMPPKNDKKISLAEE 988
             L+  +  S SE+         S LESVL  N D++                  I  AEE
Sbjct: 234  -LMNNNEASSSEHEQ-------SSLESVLTLNEDNQ------------------IHPAEE 267

Query: 987  LGSLISFDTIETPAPMNLIRQPSPPPVLADVQDLIPTTSPPVTEAEYGRDDPSSNGFSCS 808
            LGSLISF+T ETP    +IRQPSPPPVLADVQDLIP T   V+E     + PS++G  CS
Sbjct: 268  LGSLISFETTETPHT-EIIRQPSPPPVLADVQDLIPETPTQVSEVNGQMETPSTDGLVCS 326

Query: 807  ESPMELHISTTMMDRFMKLAKSNTNKNLETCGILAGSLKNRKFYITALIIPKQESTSDSC 628
            E+P+E+HIST +M+ F+KLAKSNT +NLETCGILAGSLKNRKFY+TALIIPKQESTSDSC
Sbjct: 327  EAPLEMHISTVLMESFLKLAKSNTVRNLETCGILAGSLKNRKFYVTALIIPKQESTSDSC 386

Query: 627  QATNEEEIFDVQDKRSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPESVAIVMAPRD 448
            Q TNEEEIF+VQDK+SLFPLGWIHTHPTQSCFMSS+D+HTHYSYQ MLPE++AIVMAP+D
Sbjct: 387  QTTNEEEIFEVQDKQSLFPLGWIHTHPTQSCFMSSVDLHTHYSYQTMLPEAIAIVMAPKD 446

Query: 447  TSRNHGIFRLTTPGGMGVIRNCPHRGFHSHDPPSDGSPIYKQCTDVYLNPDVKFDVIDLR 268
             S+ HGIFRLTTPGGM VIR+C  RGFH H  PSDG PIYK CTDVY+NP++ FDVIDLR
Sbjct: 447  ASKKHGIFRLTTPGGMSVIRHCDQRGFHPHHSPSDGGPIYKSCTDVYMNPNIHFDVIDLR 506


>ref|XP_003528952.1| PREDICTED: AMSH-like ubiquitin thiolesterase 1-like [Glycine max]
          Length = 519

 Score =  622 bits (1604), Expect = e-175
 Identities = 332/538 (61%), Positives = 400/538 (74%), Gaps = 20/538 (3%)
 Frame = -1

Query: 1821 RINVAASTRTLDVDNRISLRYYFRIADNILKQANIFRAEKNYIDLYIMLLRFSSLVTETI 1642
            RIN+AAS + +DVDNRISLR+Y+RIADNIL+QA+IFRAEKN IDLY+MLLRFSSLV+ETI
Sbjct: 10   RINIAASAQKVDVDNRISLRFYYRIADNILRQADIFRAEKNIIDLYVMLLRFSSLVSETI 69

Query: 1641 PRHRDYRASSQREKNYLKKRSLDAVIELENIKPTVERMIDELSKKHTHSIKGREITNSN- 1465
            PRHRDYR+S  R+K  LKK+ L ++ ELEN+KP V++ I+EL+ K  +   G+   +SN 
Sbjct: 70   PRHRDYRSSPPRQKESLKKKLLISLNELENLKPVVQQKINELNSKLAYQQNGQGKFSSNN 129

Query: 1464 -----------------VKAQRPAVPEYVHK-NQIQQFSHTSSVEPQFRRLSLSIPLPKE 1339
                             +KA RP   E+V++ ++ Q FS+   VE   RRLSL++P PKE
Sbjct: 130  SLDFSPVKKQTSASYGLIKAVRPTAGEFVYQGSRSQPFSYVRPVEEHARRLSLTLPPPKE 189

Query: 1338 ETLSRHSILGPNGLRGQWQPPTSFKAISYPSDIDFTPVEIPSDLPRDKSQHHLVENGSLL 1159
            ETLSRHSILGPNGL+GQW+PP   K I YPS+ID +PVE+PS         H +E+ SL 
Sbjct: 190  ETLSRHSILGPNGLKGQWRPPIIDKGIKYPSNIDLSPVELPS-------LQHSLEDESLK 242

Query: 1158 KKDSISISENLPEKDSSITSDLESVLLPNND-DKMPQNEELGSLMPPKNDKKISLAEELG 982
            KKD+ SI+E+         S+L+S+L  + D    PQ +       P++D      +E  
Sbjct: 243  KKDN-SIAEHHK-------SELDSILTQSEDCQPHPQPQ-------PQHD------QEPP 281

Query: 981  SLISFDTIETPAPMNLIRQPSPPPVLADVQDLIPTTSPPVTEAEYGRDDPSSNGFSCSES 802
            SLISF+T ET A + +IRQPSPPPVLA+VQDL+P  SP V EA    + PSS+     E+
Sbjct: 282  SLISFETTETSARIEVIRQPSPPPVLAEVQDLVPAVSPCVNEAGCKTEIPSSDSSVHVEA 341

Query: 801  PMELHISTTMMDRFMKLAKSNTNKNLETCGILAGSLKNRKFYITALIIPKQESTSDSCQA 622
            PM+LHIST +M+ FMKLAKSNT KNLETCG+LAG LKNRKFYITALIIPKQESTSDSCQ 
Sbjct: 342  PMQLHISTALMESFMKLAKSNTKKNLETCGVLAGLLKNRKFYITALIIPKQESTSDSCQT 401

Query: 621  TNEEEIFDVQDKRSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPESVAIVMAPRDTS 442
            TNEEEIF+VQDKRSLFPLGWIHTHPTQSCFMSSID+HTHYSYQIMLPESVAIVMAPRD+S
Sbjct: 402  TNEEEIFEVQDKRSLFPLGWIHTHPTQSCFMSSIDLHTHYSYQIMLPESVAIVMAPRDSS 461

Query: 441  RNHGIFRLTTPGGMGVIRNCPHRGFHSHDPPSDGSPIYKQCTDVYLNPDVKFDVIDLR 268
            RNHGIFRLT PGGM VI+ C  RGFH H  P DG PIYK CTDVY+NPD+KF+VIDLR
Sbjct: 462  RNHGIFRLTAPGGMSVIKQCDQRGFHPHSQPPDGGPIYKTCTDVYMNPDLKFEVIDLR 519


>ref|XP_003534164.1| PREDICTED: AMSH-like ubiquitin thiolesterase 1-like [Glycine max]
          Length = 520

 Score =  619 bits (1597), Expect = e-175
 Identities = 327/537 (60%), Positives = 396/537 (73%), Gaps = 19/537 (3%)
 Frame = -1

Query: 1821 RINVAASTRTLDVDNRISLRYYFRIADNILKQANIFRAEKNYIDLYIMLLRFSSLVTETI 1642
            RIN+AAS + +DVDNRISLR+Y+RIADNIL+QA+IFRAEKN IDLY+MLLRFSSLV+ETI
Sbjct: 11   RINIAASAQKVDVDNRISLRFYYRIADNILRQADIFRAEKNIIDLYVMLLRFSSLVSETI 70

Query: 1641 PRHRDYRASSQREKNYLKKRSLDAVIELENIKPTVERMIDELSKKHTHSIKG-------- 1486
            PRHRDYR+S QR+K  LKK+ L ++ ELEN+KP V++ I+EL+ K  +   G        
Sbjct: 71   PRHRDYRSSPQRQKESLKKKLLISLNELENLKPVVQQKINELNNKFAYQQNGQGKFISNN 130

Query: 1485 -------REITNSN---VKAQRPAVPEYVHK-NQIQQFSHTSSVEPQFRRLSLSIPLPKE 1339
                   +++T+++   +KA RP   E+V++ ++ QQFS+   VE   RRLSL++P PKE
Sbjct: 131  SLDFSPVKKLTSASYGLIKAVRPTAGEFVYQGSRSQQFSYVRPVEEHVRRLSLTLPPPKE 190

Query: 1338 ETLSRHSILGPNGLRGQWQPPTSFKAISYPSDIDFTPVEIPSDLPRDKSQHHLVENGSLL 1159
            ETLSRHSILGPNGL+G W+PP   K I YPS+ID +PVE+PS           +E+ SL 
Sbjct: 191  ETLSRHSILGPNGLKGHWRPPIIDKGIKYPSNIDLSPVELPS-------LQQSMEDESLK 243

Query: 1158 KKDSISISENLPEKDSSITSDLESVLLPNNDDKMPQNEELGSLMPPKNDKKISLAEELGS 979
            KKD+ SI+E+   + +SI +  E   L  +    P  E      PP             S
Sbjct: 244  KKDN-SIAEHHKSELASILTQSEDCQLQPHPQPEPDQE------PP-------------S 283

Query: 978  LISFDTIETPAPMNLIRQPSPPPVLADVQDLIPTTSPPVTEAEYGRDDPSSNGFSCSESP 799
            LISF+T ET A + +IRQPSPPPVLA+VQDL+P  SP V EA    + PS +    +E P
Sbjct: 284  LISFETTETSAQIEVIRQPSPPPVLAEVQDLVPAVSPCVNEAGCKTEIPSLDNSVHAEDP 343

Query: 798  MELHISTTMMDRFMKLAKSNTNKNLETCGILAGSLKNRKFYITALIIPKQESTSDSCQAT 619
            ++LHIS  +M+ FMKLAKSNT KNLETCG+LAG LKNRKFYITALIIPKQESTSDSCQ T
Sbjct: 344  LQLHISAALMESFMKLAKSNTKKNLETCGVLAGLLKNRKFYITALIIPKQESTSDSCQTT 403

Query: 618  NEEEIFDVQDKRSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPESVAIVMAPRDTSR 439
            NEEEIF+VQDKRSLFPLGWIHTHPTQSCFMSSID+HTHYSYQIMLPESVAIVMAPRD+SR
Sbjct: 404  NEEEIFEVQDKRSLFPLGWIHTHPTQSCFMSSIDLHTHYSYQIMLPESVAIVMAPRDSSR 463

Query: 438  NHGIFRLTTPGGMGVIRNCPHRGFHSHDPPSDGSPIYKQCTDVYLNPDVKFDVIDLR 268
            NHGIFRLTTPGGM VI+ C  RGFH H  P DG PIYK CTDVY+NPD+KF+VIDLR
Sbjct: 464  NHGIFRLTTPGGMSVIKQCDQRGFHPHSQPPDGGPIYKTCTDVYMNPDLKFEVIDLR 520


>ref|XP_003630863.1| AMSH-like protease [Medicago truncatula] gi|355524885|gb|AET05339.1|
            AMSH-like protease [Medicago truncatula]
          Length = 511

 Score =  619 bits (1596), Expect = e-175
 Identities = 324/536 (60%), Positives = 385/536 (71%), Gaps = 19/536 (3%)
 Frame = -1

Query: 1818 INVAASTRTLDVDNRISLRYYFRIADNILKQANIFRAEKNYIDLYIMLLRFSSLVTETIP 1639
            I++AA T+ +DVDNR SLR Y+RIADNILKQA+IFRAEKN IDLYIMLLR+SSLV+ETIP
Sbjct: 9    ISIAARTQKVDVDNRFSLRIYYRIADNILKQADIFRAEKNIIDLYIMLLRYSSLVSETIP 68

Query: 1638 RHRDYRASSQREKNYLKKRSLDAVIELENIKPTVERMIDELSKKHTHSIKGREITNSN-- 1465
            RHRDYR S Q +K  L+K+ L +VIELE +KP  ++ I+EL+ + ++   GR+   SN  
Sbjct: 69   RHRDYRTSPQSKKELLRKKLLTSVIELEKLKPLAQQKINELNSRKSYQHSGRDTFRSNYS 128

Query: 1464 ----------------VKAQRPAVPEYVHK-NQIQQFSHTSSVEPQFRRLSLSIPLPKEE 1336
                            +KA R    E+V++ ++ QQ+     VE   +RLSL++P PKEE
Sbjct: 129  TGFSPVKKQTMASYDEIKAVRQTAGEFVYRGSKAQQYPCVRPVEDSMKRLSLTLPPPKEE 188

Query: 1335 TLSRHSILGPNGLRGQWQPPTSFKAISYPSDIDFTPVEIPSDLPRDKSQHHLVENGSLLK 1156
            TLSRHSILGPNGL+G WQPPTS K + YP+ ID +PVEIPS        H  +E+GS  K
Sbjct: 189  TLSRHSILGPNGLKGPWQPPTSDKGVRYPTIIDLSPVEIPS-------LHQSLEDGSQNK 241

Query: 1155 KDSISISENLPEKDSSITSDLESVLLPNNDDKMPQNEELGSLMPPKNDKKISLAEELGSL 976
            KD+     N  + +S++T                             D ++  A+E  SL
Sbjct: 242  KDNSISEHNKLDVESTLTQS--------------------------EDCQVKHADETPSL 275

Query: 975  ISFDTIETPAPMNLIRQPSPPPVLADVQDLIPTTSPPVTEAEYGRDDPSSNGFSCSESPM 796
            ISF+  E  AP+ +IRQPSPPPVLA+VQDL+PT SP V EA    + P S+ F  +ESP+
Sbjct: 276  ISFEETEDFAPIKVIRQPSPPPVLAEVQDLVPTVSPHVDEAGCKTETPLSDSFGRAESPL 335

Query: 795  ELHISTTMMDRFMKLAKSNTNKNLETCGILAGSLKNRKFYITALIIPKQESTSDSCQATN 616
            +LHIST MM  FMKLAKSNT+KNLETCGILAGSLKNRKFYITALIIPKQE+TS SCQATN
Sbjct: 336  QLHISTAMMGSFMKLAKSNTDKNLETCGILAGSLKNRKFYITALIIPKQEATSSSCQATN 395

Query: 615  EEEIFDVQDKRSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPESVAIVMAPRDTSRN 436
            EEEIF+ QDKRSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPE+VAIVMAP D+SR 
Sbjct: 396  EEEIFEAQDKRSLFPLGWIHTHPTQSCFMSSIDVHTHYSYQIMLPEAVAIVMAPTDSSRT 455

Query: 435  HGIFRLTTPGGMGVIRNCPHRGFHSHDPPSDGSPIYKQCTDVYLNPDVKFDVIDLR 268
            HGIFRLTTPGGM VIR C  RGFH HDPP DG PIY  CTDVYLNPD+KFDVIDLR
Sbjct: 456  HGIFRLTTPGGMSVIRQCQQRGFHPHDPPPDGGPIYDTCTDVYLNPDLKFDVIDLR 511