BLASTX nr result
ID: Bupleurum21_contig00001382
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00001382
(1615 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002510330.1| ubiquitin-protein ligase, putative [Ricinus ... 472 e-156
ref|XP_002302466.1| predicted protein [Populus trichocarpa] gi|2... 464 e-151
ref|XP_002281901.1| PREDICTED: F-box/FBD/LRR-repeat protein At1g... 460 e-150
emb|CAN79162.1| hypothetical protein VITISV_019244 [Vitis vinifera] 460 e-150
ref|XP_003550361.1| PREDICTED: F-box/FBD/LRR-repeat protein At1g... 454 e-147
>ref|XP_002510330.1| ubiquitin-protein ligase, putative [Ricinus communis]
gi|223551031|gb|EEF52517.1| ubiquitin-protein ligase,
putative [Ricinus communis]
Length = 426
Score = 472 bits (1214), Expect(2) = e-156
Identities = 224/333 (67%), Positives = 278/333 (83%)
Frame = -3
Query: 1427 RRDPPKVPCKMDIDVDYISNLPEHITDKVLSCLSLRDAVRTSILSRKWRYKWITLPHLIF 1248
+R+PPK PC ++ ++D IS+LP H+ D++LS LS+RDAVRTS LSRKWRYKW +PHL+F
Sbjct: 2 KREPPKFPC-LETELDRISSLPGHVLDQILSQLSIRDAVRTSALSRKWRYKWAKIPHLVF 60
Query: 1247 DNQSLLISNQDQALIRNKLINIVDHVLLLHAGPIHKFKLSHRDLQGVYDIDRWILFLSRG 1068
DN+ + I +QDQ LI++KL+NI+DHVLLLH GPI KFKLSHRDL GV DIDRWIL LSR
Sbjct: 61 DNKCVSIPSQDQTLIKDKLVNIIDHVLLLHNGPIQKFKLSHRDLLGVSDIDRWILHLSRS 120
Query: 1067 SVKEFILEIWKGHRYKLPASLYFCQKLIHLEVFNCLLQPPSTFNGFKNLKSLDLQHITMD 888
S+KEFILEIWKG RYK+P+SL+ + LIHLE+FNCLLQPP TF GF++LKSLDLQHIT+
Sbjct: 121 SIKEFILEIWKGQRYKVPSSLFSFEHLIHLELFNCLLQPPLTFKGFRSLKSLDLQHITLT 180
Query: 887 QDVFDNLISNCALLERLTLMNFDGFSHLNIHAPYLQFFDIGGVFEDVNFDTTNNLAIVSM 708
Q+VF+NLI +C LLERLTLMNFDGF+HLNI+AP LQFFDIGGV++DV F+ T L +VS+
Sbjct: 181 QNVFENLIFSCPLLERLTLMNFDGFTHLNINAPNLQFFDIGGVYDDVTFENTFQLTLVSI 240
Query: 707 GLYATAGYDQNPPLGSAGNMIKFFSPLPRIQRLEVQSLFLKYLAVGKVPERLPTPCIELN 528
GLY D+N GS+ +++FF+ LP I+RLEVQS FLKYL++G VP RLP PC +LN
Sbjct: 241 GLYVNVQNDRNVGHGSSSKLLRFFANLPHIRRLEVQSYFLKYLSIGNVPSRLPKPCFDLN 300
Query: 527 YLSLRINFNDRAESMAALCILRSSPNLQELEIL 429
YLS+RINFND E+ AALC+LRSSPN+QELE+L
Sbjct: 301 YLSIRINFNDLEENSAALCLLRSSPNVQELEML 333
Score = 107 bits (268), Expect(2) = e-156
Identities = 54/94 (57%), Positives = 74/94 (78%)
Frame = -2
Query: 318 MQARPEEQGAAGTGAGISEEYFYNCPFHQLQVVKMVGVSHAKHDLNFINFLLANSPLLEK 139
M ARPEEQ + GT E+ +N F QL++V++VG+S + +L+F+NFLL+NSP+LE+
Sbjct: 332 MLARPEEQTSLGTITNFWEDDHWNNLFGQLRLVRIVGISGIRCELDFMNFLLSNSPVLER 391
Query: 138 MTIKPVSTNGGCELLKELLRFRRASVLAEIMYLD 37
MT+KP S++GG EL+KELLRFRRAS AEI+YLD
Sbjct: 392 MTVKPASSDGGWELIKELLRFRRASARAEIIYLD 425
>ref|XP_002302466.1| predicted protein [Populus trichocarpa] gi|222844192|gb|EEE81739.1|
predicted protein [Populus trichocarpa]
Length = 505
Score = 464 bits (1193), Expect(2) = e-151
Identities = 212/333 (63%), Positives = 275/333 (82%)
Frame = -3
Query: 1427 RRDPPKVPCKMDIDVDYISNLPEHITDKVLSCLSLRDAVRTSILSRKWRYKWITLPHLIF 1248
+R+PPK PC++D ++D IS+LP H+ ++LS L +RDAVRTS L RKWRYKW +PH++F
Sbjct: 80 QREPPKFPCEIDTELDRISSLPGHVLGQILSLLPIRDAVRTSALKRKWRYKWSQIPHIVF 139
Query: 1247 DNQSLLISNQDQALIRNKLINIVDHVLLLHAGPIHKFKLSHRDLQGVYDIDRWILFLSRG 1068
D+Q + IS+QDQ I+NKL+NI+DH+LLLH GPI+KFKLSH DL V DIDRWIL LSRG
Sbjct: 140 DSQGISISSQDQTTIKNKLVNIIDHILLLHNGPIYKFKLSHCDLLAVSDIDRWILHLSRG 199
Query: 1067 SVKEFILEIWKGHRYKLPASLYFCQKLIHLEVFNCLLQPPSTFNGFKNLKSLDLQHITMD 888
S KEF+LEIWKG +YKLP+ L+ + L++LE+FNCLL+PP F GF+NLK+LDLQH+T+
Sbjct: 200 STKEFVLEIWKGQQYKLPSCLFSFKNLVYLELFNCLLKPPLAFKGFRNLKNLDLQHVTLA 259
Query: 887 QDVFDNLISNCALLERLTLMNFDGFSHLNIHAPYLQFFDIGGVFEDVNFDTTNNLAIVSM 708
Q+VF+ LIS+CA+LERLTL+NFDGF+HLNI+AP LQFFD+GGVF+DV+F+ T LA+VS+
Sbjct: 260 QEVFEKLISSCAMLERLTLINFDGFTHLNINAPNLQFFDVGGVFDDVSFENTFVLALVSI 319
Query: 707 GLYATAGYDQNPPLGSAGNMIKFFSPLPRIQRLEVQSLFLKYLAVGKVPERLPTPCIELN 528
GLY DQN GS+ +++FF LP ++RLE+QS FLKYLA+GKVP +LP PCI+LN
Sbjct: 320 GLYVNVKNDQNVSYGSSSKLLRFFVNLPHVRRLEIQSYFLKYLAIGKVPSKLPKPCIDLN 379
Query: 527 YLSLRINFNDRAESMAALCILRSSPNLQELEIL 429
YLS+R+NFND E+ AA C+LRS PNLQE+EIL
Sbjct: 380 YLSIRLNFNDYEENSAAFCLLRSCPNLQEIEIL 412
Score = 99.8 bits (247), Expect(2) = e-151
Identities = 49/92 (53%), Positives = 67/92 (72%)
Frame = -2
Query: 312 ARPEEQGAAGTGAGISEEYFYNCPFHQLQVVKMVGVSHAKHDLNFINFLLANSPLLEKMT 133
ARPEEQ G ++ + C F QL++VK+VG+S + +L+ I FLL+NSP+LEKMT
Sbjct: 413 ARPEEQAVVGPVTDFWDDDHWKCLFGQLRLVKIVGISGIRSELDCIKFLLSNSPVLEKMT 472
Query: 132 IKPVSTNGGCELLKELLRFRRASVLAEIMYLD 37
IKPVS GG EL+K+LL FRRAS+ AE+ +L+
Sbjct: 473 IKPVSNEGGWELVKQLLHFRRASIQAEVFHLE 504
>ref|XP_002281901.1| PREDICTED: F-box/FBD/LRR-repeat protein At1g13570-like [Vitis
vinifera]
Length = 416
Score = 460 bits (1183), Expect(2) = e-150
Identities = 220/323 (68%), Positives = 265/323 (82%)
Frame = -3
Query: 1397 MDIDVDYISNLPEHITDKVLSCLSLRDAVRTSILSRKWRYKWITLPHLIFDNQSLLISNQ 1218
MD+++D ISNLP HI DK+LS LS+RDAVR S+LS KWR+KW+TLP+L+FDNQ + IS+Q
Sbjct: 1 MDMELDKISNLPGHIIDKILSLLSIRDAVRMSVLSSKWRFKWVTLPYLVFDNQCVSISSQ 60
Query: 1217 DQALIRNKLINIVDHVLLLHAGPIHKFKLSHRDLQGVYDIDRWILFLSRGSVKEFILEIW 1038
DQ L++NKL+NI+DHVLLLH GPIHKF+LSHRD G DIDRWIL+LSR ++EFILEIW
Sbjct: 61 DQTLVKNKLVNIIDHVLLLHTGPIHKFRLSHRDFLGGSDIDRWILYLSRTPIREFILEIW 120
Query: 1037 KGHRYKLPASLYFCQKLIHLEVFNCLLQPPSTFNGFKNLKSLDLQHITMDQDVFDNLISN 858
KG YKLP+ LY CQ LIHLE+FN LL+PP TF GF +LKSLDLQHI M QD F+NLIS+
Sbjct: 121 KGQHYKLPSCLYNCQSLIHLELFNSLLKPPPTFKGFMSLKSLDLQHIIMAQDAFENLISS 180
Query: 857 CALLERLTLMNFDGFSHLNIHAPYLQFFDIGGVFEDVNFDTTNNLAIVSMGLYATAGYDQ 678
C LLE+ TLMNFDGF+HLNIHAP LQFFD GGVF DVNF T LA VS+GLY D+
Sbjct: 181 CPLLEKFTLMNFDGFTHLNIHAPNLQFFDTGGVFYDVNFKNTFLLATVSIGLYVDITNDK 240
Query: 677 NPPLGSAGNMIKFFSPLPRIQRLEVQSLFLKYLAVGKVPERLPTPCIELNYLSLRINFND 498
N G++ N++KFF+ LPRIQRLE+QS FLKYLA G VP +LP PCI+L +LS+RINF+
Sbjct: 241 NLADGNSRNLVKFFAHLPRIQRLEIQSYFLKYLAAGNVPGKLPAPCIDLTHLSIRINFSS 300
Query: 497 RAESMAALCILRSSPNLQELEIL 429
E++ ALC+LRSSPNLQELE+L
Sbjct: 301 VEENLTALCLLRSSPNLQELEML 323
Score = 100 bits (248), Expect(2) = e-150
Identities = 53/94 (56%), Positives = 67/94 (71%)
Frame = -2
Query: 318 MQARPEEQGAAGTGAGISEEYFYNCPFHQLQVVKMVGVSHAKHDLNFINFLLANSPLLEK 139
M ARPEEQ T + ++ +N F L+VVK+ G+S L+FINFLLA SP+LEK
Sbjct: 322 MLARPEEQTLLETSTTLWKKEHWNYSFAHLRVVKLTGISGVGPQLDFINFLLAYSPVLEK 381
Query: 138 MTIKPVSTNGGCELLKELLRFRRASVLAEIMYLD 37
MT+KP S +G EL+K+LLRFRRASV AEI+YLD
Sbjct: 382 MTVKPASGDGAWELIKDLLRFRRASVRAEIIYLD 415
>emb|CAN79162.1| hypothetical protein VITISV_019244 [Vitis vinifera]
Length = 416
Score = 460 bits (1183), Expect(2) = e-150
Identities = 220/323 (68%), Positives = 265/323 (82%)
Frame = -3
Query: 1397 MDIDVDYISNLPEHITDKVLSCLSLRDAVRTSILSRKWRYKWITLPHLIFDNQSLLISNQ 1218
MD+++D ISNLP HI DK+LS LS+RDAVR S+LS KWR+KW+TLP+L+FDNQ + IS+Q
Sbjct: 1 MDMELDKISNLPGHIIDKILSLLSIRDAVRMSVLSSKWRFKWVTLPYLVFDNQCVSISSQ 60
Query: 1217 DQALIRNKLINIVDHVLLLHAGPIHKFKLSHRDLQGVYDIDRWILFLSRGSVKEFILEIW 1038
DQ L++NKL+NI+DHVLLLH GPIHKF+LSHRD G DIDRWIL+LSR ++EFILEIW
Sbjct: 61 DQTLVKNKLVNIIDHVLLLHTGPIHKFRLSHRDFLGGSDIDRWILYLSRTPIREFILEIW 120
Query: 1037 KGHRYKLPASLYFCQKLIHLEVFNCLLQPPSTFNGFKNLKSLDLQHITMDQDVFDNLISN 858
KG YKLP+ LY CQ LIHLE+FN LL+PP TF GF +LKSLDLQHI M QD F+NLIS+
Sbjct: 121 KGQHYKLPSCLYNCQSLIHLELFNSLLKPPPTFKGFXSLKSLDLQHIIMAQDAFENLISS 180
Query: 857 CALLERLTLMNFDGFSHLNIHAPYLQFFDIGGVFEDVNFDTTNNLAIVSMGLYATAGYDQ 678
C LLE+ TLMNFDGF+HLNIHAP LQFFD GGVF DVNF T LA VS+GLY D+
Sbjct: 181 CPLLEKFTLMNFDGFTHLNIHAPNLQFFDTGGVFYDVNFKNTFLLATVSIGLYVDITNDK 240
Query: 677 NPPLGSAGNMIKFFSPLPRIQRLEVQSLFLKYLAVGKVPERLPTPCIELNYLSLRINFND 498
N G++ N++KFF+ LPRIQRLE+QS FLKYLA G VP +LP PCI+L +LS+RINF+
Sbjct: 241 NLADGNSRNLVKFFAHLPRIQRLEIQSYFLKYLAAGNVPGKLPAPCIDLTHLSIRINFSS 300
Query: 497 RAESMAALCILRSSPNLQELEIL 429
E++ ALC+LRSSPNLQELE+L
Sbjct: 301 VEENLTALCLLRSSPNLQELEML 323
Score = 100 bits (248), Expect(2) = e-150
Identities = 53/94 (56%), Positives = 67/94 (71%)
Frame = -2
Query: 318 MQARPEEQGAAGTGAGISEEYFYNCPFHQLQVVKMVGVSHAKHDLNFINFLLANSPLLEK 139
M ARPEEQ T + ++ +N F L+VVK+ G+S L+FINFLLA SP+LEK
Sbjct: 322 MLARPEEQTLLETSTTLWKKEHWNYXFAHLRVVKLTGISGVGPQLDFINFLLAYSPVLEK 381
Query: 138 MTIKPVSTNGGCELLKELLRFRRASVLAEIMYLD 37
MT+KP S +G EL+K+LLRFRRASV AEI+YLD
Sbjct: 382 MTVKPASGDGAWELIKDLLRFRRASVRAEIIYLD 415
>ref|XP_003550361.1| PREDICTED: F-box/FBD/LRR-repeat protein At1g13570-like [Glycine max]
Length = 427
Score = 454 bits (1169), Expect(2) = e-147
Identities = 219/333 (65%), Positives = 269/333 (80%)
Frame = -3
Query: 1427 RRDPPKVPCKMDIDVDYISNLPEHITDKVLSCLSLRDAVRTSILSRKWRYKWITLPHLIF 1248
RR+ P+ PC D++ D IS LP H+ D+VLS LS+R+AVRTS+LS KWRYKW TLP L+F
Sbjct: 2 RREQPRSPCLTDVEPDRISCLPGHVIDQVLSHLSIREAVRTSVLSSKWRYKWATLPILVF 61
Query: 1247 DNQSLLISNQDQALIRNKLINIVDHVLLLHAGPIHKFKLSHRDLQGVYDIDRWILFLSRG 1068
D + +++QD +I+NKL+ I+DHVLLLH+GPI+KFKLSHRDL GV DIDRW L L R
Sbjct: 62 DTHCVSVASQDHMIIKNKLLRIIDHVLLLHSGPINKFKLSHRDLIGVTDIDRWTLHLCRK 121
Query: 1067 SVKEFILEIWKGHRYKLPASLYFCQKLIHLEVFNCLLQPPSTFNGFKNLKSLDLQHITMD 888
S+KEF+LEIWKG RYK+ + L+ CQ L HLE+FNC L+PPSTF GFKNLKSLDLQH+T+
Sbjct: 122 SIKEFVLEIWKGQRYKIHSCLFSCQSLTHLELFNCWLKPPSTFQGFKNLKSLDLQHVTLA 181
Query: 887 QDVFDNLISNCALLERLTLMNFDGFSHLNIHAPYLQFFDIGGVFEDVNFDTTNNLAIVSM 708
QDVF+NLIS+C LLERLTLMNFDGF++LNI AP L FFDIGG FED++F+ T LA+VS+
Sbjct: 182 QDVFENLISSCPLLERLTLMNFDGFTNLNIDAPNLLFFDIGGKFEDISFENTFQLAVVSI 241
Query: 707 GLYATAGYDQNPPLGSAGNMIKFFSPLPRIQRLEVQSLFLKYLAVGKVPERLPTPCIELN 528
GLY +Q+ G + N++KFF LP +QRLEVQS FLKYLAVG VP +LP PCI+L+
Sbjct: 242 GLYLNFEINQSRLHGGSSNLLKFFVHLPHVQRLEVQSYFLKYLAVGVVPIKLPRPCIDLS 301
Query: 527 YLSLRINFNDRAESMAALCILRSSPNLQELEIL 429
YLS+RINFND E A+LC+LRSSPNLQELEIL
Sbjct: 302 YLSIRINFNDLKEISASLCLLRSSPNLQELEIL 334
Score = 97.4 bits (241), Expect(2) = e-147
Identities = 52/92 (56%), Positives = 69/92 (75%)
Frame = -2
Query: 312 ARPEEQGAAGTGAGISEEYFYNCPFHQLQVVKMVGVSHAKHDLNFINFLLANSPLLEKMT 133
ARPEEQ T E+ +++CP QL+ VK+ G+S K +L+FINFLL +SP+LE+MT
Sbjct: 335 ARPEEQTVLLTHTYCWEDVYFSCPVMQLRYVKIDGISGIKPELDFINFLLLHSPVLERMT 394
Query: 132 IKPVSTNGGCELLKELLRFRRASVLAEIMYLD 37
+KPV+ N G EL+KELLRFRRAS AEI+YL+
Sbjct: 395 VKPVA-NVGLELMKELLRFRRASGQAEIIYLE 425