BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00001354
         (666 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002274569.1| PREDICTED: probable E3 ubiquitin-protein lig...   389   e-106
emb|CAN67581.1| hypothetical protein VITISV_010346 [Vitis vinifera]   389   e-106
ref|XP_004168089.1| PREDICTED: probable E3 ubiquitin-protein lig...   386   e-105
ref|XP_004146396.1| PREDICTED: probable E3 ubiquitin-protein lig...   386   e-105
ref|XP_002866579.1| UVB-resistance 8 [Arabidopsis lyrata subsp. ...   379   e-103

>ref|XP_002274569.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC1 [Vitis
           vinifera] gi|296082603|emb|CBI21608.3| unnamed protein
           product [Vitis vinifera]
          Length = 445

 Score =  389 bits (999), Expect = e-106
 Identities = 177/217 (81%), Positives = 196/217 (90%)
 Frame = +3

Query: 3   WGRYGNLGLGDRNDRLIPERVVTNDDEEMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
           WGRYGNLGLGDRNDRL+PE+V T +  +MV VACGWRHTISVSSSGGLYTYGWSKYGQLG
Sbjct: 199 WGRYGNLGLGDRNDRLVPEKVSTVEGVKMVKVACGWRHTISVSSSGGLYTYGWSKYGQLG 258

Query: 183 HGDFEDHLIPHKLDALSGEVISQISGGWRHTMAVTSNGKLYGWGWNKFGQVGVGDNLDHC 362
           HGDFEDHL PHKL+AL   +IS+ISGGWRHTMA+TS+GKLYGWGWNKFGQVGVGDN+DHC
Sbjct: 259 HGDFEDHLTPHKLEALQENLISEISGGWRHTMALTSDGKLYGWGWNKFGQVGVGDNVDHC 318

Query: 363 SPMPVNFPHEQKVVQISCGWRHTLAVTDKHNVFSWGRGTNGQLGHKESIDRNIPKIIEAL 542
           SP+ V FPHEQKVV ISCGWRHTLAVT++ NVFSWGRGTNGQLGH ESIDRNIPK+IE L
Sbjct: 319 SPVQVKFPHEQKVVHISCGWRHTLAVTERQNVFSWGRGTNGQLGHGESIDRNIPKMIEVL 378

Query: 543 SVDGSNGQLIESSNIDPSIGKISVSPSDRYAVVPDES 653
           S DGS GQ IE+S +DPS G+I VSPS+RYAVVPDE+
Sbjct: 379 SADGSGGQQIETSKVDPSTGRIWVSPSERYAVVPDET 415



 Score =  148 bits (373), Expect = 1e-33
 Identities = 67/180 (37%), Positives = 107/180 (59%)
 Frame = +3

Query: 3   WGRYGNLGLGDRNDRLIPERVVTNDDEEMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
           WG +G LG G+ +D   P+ +       +  +ACG  H ++V+  G + ++G ++ GQLG
Sbjct: 95  WGDFGRLGHGNSSDLFTPQPIKALHGLRIKQIACGDSHCLAVTMDGEVQSWGRNQNGQLG 154

Query: 183 HGDFEDHLIPHKLDALSGEVISQISGGWRHTMAVTSNGKLYGWGWNKFGQVGVGDNLDHC 362
            G  ED L+P K+ A  G  +  ++ G  HT AVT +G+LYGWGW ++G +G+GD  D  
Sbjct: 155 LGTTEDSLVPQKIQAFQGVSVKMVAAGAEHTAAVTEDGELYGWGWGRYGNLGLGDRNDRL 214

Query: 363 SPMPVNFPHEQKVVQISCGWRHTLAVTDKHNVFSWGRGTNGQLGHKESIDRNIPKIIEAL 542
            P  V+     K+V+++CGWRHT++V+    ++++G    GQLGH +  D   P  +EAL
Sbjct: 215 VPEKVSTVEGVKMVKVACGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLTPHKLEAL 274



 Score =  115 bits (288), Expect = 8e-24
 Identities = 60/152 (39%), Positives = 91/152 (59%), Gaps = 1/152 (0%)
 Frame = +3

Query: 87  MVLVACGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLIPHKLDALSGEVISQISGGW 266
           ++L++ G  H++++ S   + ++G  + GQLGHGD ED L P  L AL G  +  ++ G 
Sbjct: 18  VLLISAGASHSVALLSGNVVCSWGRGEDGQLGHGDAEDRLSPTYLSALDGHEVVSVTCGA 77

Query: 267 RHTMAVT-SNGKLYGWGWNKFGQVGVGDNLDHCSPMPVNFPHEQKVVQISCGWRHTLAVT 443
            HT A + S  ++Y WGW  FG++G G++ D  +P P+   H  ++ QI+CG  H LAVT
Sbjct: 78  DHTTAYSESFTQVYSWGWGDFGRLGHGNSSDLFTPQPIKALHGLRIKQIACGDSHCLAVT 137

Query: 444 DKHNVFSWGRGTNGQLGHKESIDRNIPKIIEA 539
               V SWGR  NGQLG   + D  +P+ I+A
Sbjct: 138 MDGEVQSWGRNQNGQLGLGTTEDSLVPQKIQA 169


>emb|CAN67581.1| hypothetical protein VITISV_010346 [Vitis vinifera]
          Length = 423

 Score =  389 bits (999), Expect = e-106
 Identities = 177/217 (81%), Positives = 196/217 (90%)
 Frame = +3

Query: 3   WGRYGNLGLGDRNDRLIPERVVTNDDEEMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
           WGRYGNLGLGDRNDRL+PE+V T +  +MV VACGWRHTISVSSSGGLYTYGWSKYGQLG
Sbjct: 187 WGRYGNLGLGDRNDRLVPEKVSTVEGVKMVKVACGWRHTISVSSSGGLYTYGWSKYGQLG 246

Query: 183 HGDFEDHLIPHKLDALSGEVISQISGGWRHTMAVTSNGKLYGWGWNKFGQVGVGDNLDHC 362
           HGDFEDHL PHKL+AL   +IS+ISGGWRHTMA+TS+GKLYGWGWNKFGQVGVGDN+DHC
Sbjct: 247 HGDFEDHLTPHKLEALQENLISEISGGWRHTMALTSDGKLYGWGWNKFGQVGVGDNVDHC 306

Query: 363 SPMPVNFPHEQKVVQISCGWRHTLAVTDKHNVFSWGRGTNGQLGHKESIDRNIPKIIEAL 542
           SP+ V FPHEQKVV ISCGWRHTLAVT++ NVFSWGRGTNGQLGH ESIDRNIPK+IE L
Sbjct: 307 SPVQVKFPHEQKVVHISCGWRHTLAVTERQNVFSWGRGTNGQLGHGESIDRNIPKMIEVL 366

Query: 543 SVDGSNGQLIESSNIDPSIGKISVSPSDRYAVVPDES 653
           S DGS GQ IE+S +DPS G+I VSPS+RYAVVPDE+
Sbjct: 367 SADGSGGQQIETSKVDPSTGRIWVSPSERYAVVPDET 403



 Score =  136 bits (342), Expect = 4e-30
 Identities = 76/223 (34%), Positives = 109/223 (48%), Gaps = 43/223 (19%)
 Frame = +3

Query: 3   WGRY--GNLGLGDRNDRLIPERVVTNDDEEMVLVACGWRHTISVSSS------------- 137
           WGR   G LG GD  DRL P  +   D  E+V V CG  HT + S S             
Sbjct: 40  WGRGEDGQLGHGDAEDRLSPTYLSALDGHEVVSVTCGADHTTAYSESFTQVYSWGCDLFT 99

Query: 138 ----------------------------GGLYTYGWSKYGQLGHGDFEDHLIPHKLDALS 233
                                       G + ++G ++ GQLG G  ED L+P K+ A  
Sbjct: 100 PQPIKALHGLRIKQIACGDSHCLAVTMDGEVQSWGRNQNGQLGLGTTEDSLVPQKIQAFQ 159

Query: 234 GEVISQISGGWRHTMAVTSNGKLYGWGWNKFGQVGVGDNLDHCSPMPVNFPHEQKVVQIS 413
           G  +  ++ G  HT AVT +G+LYGWGW ++G +G+GD  D   P  V+     K+V+++
Sbjct: 160 GVSVKMVAAGAEHTAAVTEDGELYGWGWGRYGNLGLGDRNDRLVPEKVSTVEGVKMVKVA 219

Query: 414 CGWRHTLAVTDKHNVFSWGRGTNGQLGHKESIDRNIPKIIEAL 542
           CGWRHT++V+    ++++G    GQLGH +  D   P  +EAL
Sbjct: 220 CGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLTPHKLEAL 262



 Score = 92.8 bits (229), Expect = 5e-17
 Identities = 54/152 (35%), Positives = 82/152 (53%), Gaps = 1/152 (0%)
 Frame = +3

Query: 87  MVLVACGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLIPHKLDALSGEVISQISGGW 266
           ++L++ G  H++++     + ++G  + GQLGHGD ED L P  L AL G  +  ++ G 
Sbjct: 18  VLLISAGASHSVALLXGNVVCSWGRGEDGQLGHGDAEDRLSPTYLSALDGHEVVSVTCGA 77

Query: 267 RHTMAVT-SNGKLYGWGWNKFGQVGVGDNLDHCSPMPVNFPHEQKVVQISCGWRHTLAVT 443
            HT A + S  ++Y WG + F            +P P+   H  ++ QI+CG  H LAVT
Sbjct: 78  DHTTAYSESFTQVYSWGCDLF------------TPQPIKALHGLRIKQIACGDSHCLAVT 125

Query: 444 DKHNVFSWGRGTNGQLGHKESIDRNIPKIIEA 539
               V SWGR  NGQLG   + D  +P+ I+A
Sbjct: 126 MDGEVQSWGRNQNGQLGLGTTEDSLVPQKIQA 157


>ref|XP_004168089.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC3-like, partial
           [Cucumis sativus]
          Length = 302

 Score =  386 bits (991), Expect = e-105
 Identities = 172/217 (79%), Positives = 197/217 (90%)
 Frame = +3

Query: 3   WGRYGNLGLGDRNDRLIPERVVTNDDEEMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
           WGRYGNLGLGDRNDRL+P++V + D ++MV+VACGWRHTISVSS GGLYTYGWSKYGQLG
Sbjct: 55  WGRYGNLGLGDRNDRLVPQKVSSVDGDKMVMVACGWRHTISVSSLGGLYTYGWSKYGQLG 114

Query: 183 HGDFEDHLIPHKLDALSGEVISQISGGWRHTMAVTSNGKLYGWGWNKFGQVGVGDNLDHC 362
           HGDFED L+PH+L+AL G+ ISQISGGWRHTMA+T++GKLYGWGWNKFGQVG GDN+DHC
Sbjct: 115 HGDFEDRLVPHRLEALRGDNISQISGGWRHTMALTTDGKLYGWGWNKFGQVGAGDNIDHC 174

Query: 363 SPMPVNFPHEQKVVQISCGWRHTLAVTDKHNVFSWGRGTNGQLGHKESIDRNIPKIIEAL 542
           SP+ + FP +QKV+QISCGWRHTLAVTDK NVFSWGRGTNGQLGH ES+DRN P I+EAL
Sbjct: 175 SPIQIKFPQDQKVIQISCGWRHTLAVTDKQNVFSWGRGTNGQLGHGESVDRNTPMILEAL 234

Query: 543 SVDGSNGQLIESSNIDPSIGKISVSPSDRYAVVPDES 653
           SVDG +GQ IESSNIDPS GK  VSP++RYA+VPDES
Sbjct: 235 SVDGCSGQQIESSNIDPSSGKTWVSPTERYAIVPDES 271



 Score =  122 bits (306), Expect = 6e-26
 Identities = 55/133 (41%), Positives = 83/133 (62%)
 Frame = +3

Query: 153 YGWSKYGQLGHGDFEDHLIPHKLDALSGEVISQISGGWRHTMAVTSNGKLYGWGWNKFGQ 332
           +G ++ GQLG G  ED L+P K+ A  G  I  ++ G  HT AVT +G+L+GWGW ++G 
Sbjct: 1   WGRNQNGQLGLGTTEDSLVPQKIQAFEGISIKMVAAGAEHTAAVTEDGELFGWGWGRYGN 60

Query: 333 VGVGDNLDHCSPMPVNFPHEQKVVQISCGWRHTLAVTDKHNVFSWGRGTNGQLGHKESID 512
           +G+GD  D   P  V+     K+V ++CGWRHT++V+    ++++G    GQLGH +  D
Sbjct: 61  LGLGDRNDRLVPQKVSSVDGDKMVMVACGWRHTISVSSLGGLYTYGWSKYGQLGHGDFED 120

Query: 513 RNIPKIIEALSVD 551
           R +P  +EAL  D
Sbjct: 121 RLVPHRLEALRGD 133


>ref|XP_004146396.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC1-like [Cucumis
           sativus]
          Length = 438

 Score =  386 bits (991), Expect = e-105
 Identities = 172/217 (79%), Positives = 197/217 (90%)
 Frame = +3

Query: 3   WGRYGNLGLGDRNDRLIPERVVTNDDEEMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
           WGRYGNLGLGDRNDRL+P++V + D ++MV+VACGWRHTISVSS GGLYTYGWSKYGQLG
Sbjct: 191 WGRYGNLGLGDRNDRLVPQKVSSVDGDKMVMVACGWRHTISVSSLGGLYTYGWSKYGQLG 250

Query: 183 HGDFEDHLIPHKLDALSGEVISQISGGWRHTMAVTSNGKLYGWGWNKFGQVGVGDNLDHC 362
           HGDFED L+PH+L+AL G+ ISQISGGWRHTMA+T++GKLYGWGWNKFGQVG GDN+DHC
Sbjct: 251 HGDFEDRLVPHRLEALRGDNISQISGGWRHTMALTTDGKLYGWGWNKFGQVGAGDNIDHC 310

Query: 363 SPMPVNFPHEQKVVQISCGWRHTLAVTDKHNVFSWGRGTNGQLGHKESIDRNIPKIIEAL 542
           SP+ + FP +QKV+QISCGWRHTLAVTDK NVFSWGRGTNGQLGH ES+DRN P I+EAL
Sbjct: 311 SPIQIKFPQDQKVIQISCGWRHTLAVTDKQNVFSWGRGTNGQLGHGESVDRNTPMILEAL 370

Query: 543 SVDGSNGQLIESSNIDPSIGKISVSPSDRYAVVPDES 653
           SVDG +GQ IESSNIDPS GK  VSP++RYA+VPDES
Sbjct: 371 SVDGCSGQQIESSNIDPSSGKTWVSPTERYAIVPDES 407



 Score =  152 bits (383), Expect = 7e-35
 Identities = 70/183 (38%), Positives = 111/183 (60%)
 Frame = +3

Query: 3   WGRYGNLGLGDRNDRLIPERVVTNDDEEMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
           WG +G LG G+ +D L P+ +      ++  +ACG  H ++V+  G + ++G ++ GQLG
Sbjct: 87  WGDFGRLGHGNSSDLLTPKPIKALHGLKIRQIACGDSHCLAVTMEGEVQSWGRNQNGQLG 146

Query: 183 HGDFEDHLIPHKLDALSGEVISQISGGWRHTMAVTSNGKLYGWGWNKFGQVGVGDNLDHC 362
            G  ED L+P K+ A  G  I  ++ G  HT AVT +G+L+GWGW ++G +G+GD  D  
Sbjct: 147 LGTTEDSLVPQKIQAFEGISIKMVAAGAEHTAAVTEDGELFGWGWGRYGNLGLGDRNDRL 206

Query: 363 SPMPVNFPHEQKVVQISCGWRHTLAVTDKHNVFSWGRGTNGQLGHKESIDRNIPKIIEAL 542
            P  V+     K+V ++CGWRHT++V+    ++++G    GQLGH +  DR +P  +EAL
Sbjct: 207 VPQKVSSVDGDKMVMVACGWRHTISVSSLGGLYTYGWSKYGQLGHGDFEDRLVPHRLEAL 266

Query: 543 SVD 551
             D
Sbjct: 267 RGD 269



 Score =  117 bits (293), Expect = 2e-24
 Identities = 61/158 (38%), Positives = 94/158 (59%), Gaps = 1/158 (0%)
 Frame = +3

Query: 69  TNDDEEMVLVACGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLIPHKLDALSGEVIS 248
           T+    ++ ++ G  H++++ S   + ++G  + GQLGHGD ED L P +L AL G  I 
Sbjct: 4   TSPVRRVLFISAGASHSVALLSGNIVCSWGRGEDGQLGHGDAEDRLSPTQLSALDGHEIV 63

Query: 249 QISGGWRHTMAVT-SNGKLYGWGWNKFGQVGVGDNLDHCSPMPVNFPHEQKVVQISCGWR 425
            ++ G  HT A + +  ++Y WGW  FG++G G++ D  +P P+   H  K+ QI+CG  
Sbjct: 64  SVTCGADHTTAYSVARTEVYSWGWGDFGRLGHGNSSDLLTPKPIKALHGLKIRQIACGDS 123

Query: 426 HTLAVTDKHNVFSWGRGTNGQLGHKESIDRNIPKIIEA 539
           H LAVT +  V SWGR  NGQLG   + D  +P+ I+A
Sbjct: 124 HCLAVTMEGEVQSWGRNQNGQLGLGTTEDSLVPQKIQA 161


>ref|XP_002866579.1| UVB-resistance 8 [Arabidopsis lyrata subsp. lyrata]
           gi|297312414|gb|EFH42838.1| UVB-resistance 8
           [Arabidopsis lyrata subsp. lyrata]
          Length = 440

 Score =  379 bits (972), Expect = e-103
 Identities = 174/217 (80%), Positives = 195/217 (89%)
 Frame = +3

Query: 3   WGRYGNLGLGDRNDRLIPERVVTNDDEEMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
           WGRYGNLGLGDRNDRL+PERV +   E+M +VACGWRHTISVS SG LYTYGWSKYGQLG
Sbjct: 198 WGRYGNLGLGDRNDRLVPERVTSAGGEKMSMVACGWRHTISVSYSGALYTYGWSKYGQLG 257

Query: 183 HGDFEDHLIPHKLDALSGEVISQISGGWRHTMAVTSNGKLYGWGWNKFGQVGVGDNLDHC 362
           HGD EDHLIPHKL+ALS   ISQISGGWRHTMA+TS+GKLYGWGWNKFGQVGVG+NLD C
Sbjct: 258 HGDLEDHLIPHKLEALSNSFISQISGGWRHTMALTSDGKLYGWGWNKFGQVGVGNNLDQC 317

Query: 363 SPMPVNFPHEQKVVQISCGWRHTLAVTDKHNVFSWGRGTNGQLGHKESIDRNIPKIIEAL 542
           SP+ V FP +QKVVQ+SCGWRHTLAVT+++NVF+WGRGTNGQLG  ES+DRN PKIIEAL
Sbjct: 318 SPVQVRFPDDQKVVQVSCGWRHTLAVTERNNVFAWGRGTNGQLGIGESVDRNFPKIIEAL 377

Query: 543 SVDGSNGQLIESSNIDPSIGKISVSPSDRYAVVPDES 653
           SVDG++GQ IESSNIDPS GK  VSP++RYAVVPDE+
Sbjct: 378 SVDGASGQHIESSNIDPSSGKSWVSPAERYAVVPDET 414



 Score =  147 bits (370), Expect = 2e-33
 Identities = 70/181 (38%), Positives = 106/181 (58%)
 Frame = +3

Query: 3   WGRYGNLGLGDRNDRLIPERVVTNDDEEMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
           WG +G LG G+ +D   P  +       +  +ACG  H ++V+  G + ++G ++ GQLG
Sbjct: 94  WGDFGRLGHGNSSDLFTPLPIKALHGIRIKQIACGDSHCLAVTMEGEVQSWGRNQNGQLG 153

Query: 183 HGDFEDHLIPHKLDALSGEVISQISGGWRHTMAVTSNGKLYGWGWNKFGQVGVGDNLDHC 362
            GD ED L+P K+ A  G  I  ++ G  HT AVT +G LYGWGW ++G +G+GD  D  
Sbjct: 154 LGDTEDSLVPQKIQAFEGIRIKMVAAGAEHTAAVTEDGDLYGWGWGRYGNLGLGDRNDRL 213

Query: 363 SPMPVNFPHEQKVVQISCGWRHTLAVTDKHNVFSWGRGTNGQLGHKESIDRNIPKIIEAL 542
            P  V     +K+  ++CGWRHT++V+    ++++G    GQLGH +  D  IP  +EAL
Sbjct: 214 VPERVTSAGGEKMSMVACGWRHTISVSYSGALYTYGWSKYGQLGHGDLEDHLIPHKLEAL 273

Query: 543 S 545
           S
Sbjct: 274 S 274



 Score =  120 bits (301), Expect = 2e-25
 Identities = 61/159 (38%), Positives = 98/159 (61%), Gaps = 1/159 (0%)
 Frame = +3

Query: 66  VTNDDEEMVLVACGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLIPHKLDALSGEVI 245
           VT     +++++ G  H++++ S   + ++G  + GQLGHGD ED   P +L AL G  I
Sbjct: 10  VTAPPRTVLIISAGASHSVALLSGDIVCSWGRGEDGQLGHGDAEDRPSPTQLSALDGHQI 69

Query: 246 SQISGGWRHTMAVTSNG-KLYGWGWNKFGQVGVGDNLDHCSPMPVNFPHEQKVVQISCGW 422
             ++ G  HT+A + +G ++Y WGW  FG++G G++ D  +P+P+   H  ++ QI+CG 
Sbjct: 70  VSVTCGADHTVAYSQSGMEVYSWGWGDFGRLGHGNSSDLFTPLPIKALHGIRIKQIACGD 129

Query: 423 RHTLAVTDKHNVFSWGRGTNGQLGHKESIDRNIPKIIEA 539
            H LAVT +  V SWGR  NGQLG  ++ D  +P+ I+A
Sbjct: 130 SHCLAVTMEGEVQSWGRNQNGQLGLGDTEDSLVPQKIQA 168



 Score = 64.7 bits (156), Expect = 2e-08
 Identities = 37/98 (37%), Positives = 54/98 (55%), Gaps = 1/98 (1%)
 Frame = +3

Query: 252 ISGGWRHTMAVTSNGKLYGWGWNKFGQVGVGDNLDHCSPMPVNFPHEQKVVQISCGWRHT 431
           IS G  H++A+ S   +  WG  + GQ+G GD  D  SP  ++     ++V ++CG  HT
Sbjct: 20  ISAGASHSVALLSGDIVCSWGRGEDGQLGHGDAEDRPSPTQLSALDGHQIVSVTCGADHT 79

Query: 432 LAVTDKH-NVFSWGRGTNGQLGHKESIDRNIPKIIEAL 542
           +A +     V+SWG G  G+LGH  S D   P  I+AL
Sbjct: 80  VAYSQSGMEVYSWGWGDFGRLGHGNSSDLFTPLPIKAL 117