BLASTX nr result
ID: Bupleurum21_contig00001352
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00001352
(666 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002274569.1| PREDICTED: probable E3 ubiquitin-protein lig... 390 e-106
emb|CAN67581.1| hypothetical protein VITISV_010346 [Vitis vinifera] 390 e-106
ref|XP_004168089.1| PREDICTED: probable E3 ubiquitin-protein lig... 384 e-104
ref|XP_004146396.1| PREDICTED: probable E3 ubiquitin-protein lig... 384 e-104
ref|XP_002309939.1| predicted protein [Populus trichocarpa] gi|2... 379 e-103
>ref|XP_002274569.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC1 [Vitis
vinifera] gi|296082603|emb|CBI21608.3| unnamed protein
product [Vitis vinifera]
Length = 445
Score = 390 bits (1002), Expect = e-106
Identities = 178/216 (82%), Positives = 196/216 (90%)
Frame = +3
Query: 3 WGRYGNLGLGDRNDRLVPEKVATVDDEKMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
WGRYGNLGLGDRNDRLVPEKV+TV+ KMV VACGWRHTISVSSSGGLYTYGWSKYGQLG
Sbjct: 199 WGRYGNLGLGDRNDRLVPEKVSTVEGVKMVKVACGWRHTISVSSSGGLYTYGWSKYGQLG 258
Query: 183 HGDFEDHLIPHKLHALHGELISQISGGWRHTMALTSNGELYGWGWNKFGQVGVGDDLDHC 362
HGDFEDHL PHKL AL LIS+ISGGWRHTMALTS+G+LYGWGWNKFGQVGVGD++DHC
Sbjct: 259 HGDFEDHLTPHKLEALQENLISEISGGWRHTMALTSDGKLYGWGWNKFGQVGVGDNVDHC 318
Query: 363 SPMLVTFPHDQKVVQISCGWRHTLAVTERQNVYSWGRGTNGQLGHMESIDRNVPKLIEAL 542
SP+ V FPH+QKVV ISCGWRHTLAVTERQNV+SWGRGTNGQLGH ESIDRN+PK+IE L
Sbjct: 319 SPVQVKFPHEQKVVHISCGWRHTLAVTERQNVFSWGRGTNGQLGHGESIDRNIPKMIEVL 378
Query: 543 SVDGSNGQLIESSNIDPSIGKLSVLPSDRYAVVPDD 650
S DGS GQ IE+S +DPS G++ V PS+RYAVVPD+
Sbjct: 379 SADGSGGQQIETSKVDPSTGRIWVSPSERYAVVPDE 414
Score = 152 bits (385), Expect = 4e-35
Identities = 68/180 (37%), Positives = 109/180 (60%)
Frame = +3
Query: 3 WGRYGNLGLGDRNDRLVPEKVATVDDEKMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
WG +G LG G+ +D P+ + + ++ +ACG H ++V+ G + ++G ++ GQLG
Sbjct: 95 WGDFGRLGHGNSSDLFTPQPIKALHGLRIKQIACGDSHCLAVTMDGEVQSWGRNQNGQLG 154
Query: 183 HGDFEDHLIPHKLHALHGELISQISGGWRHTMALTSNGELYGWGWNKFGQVGVGDDLDHC 362
G ED L+P K+ A G + ++ G HT A+T +GELYGWGW ++G +G+GD D
Sbjct: 155 LGTTEDSLVPQKIQAFQGVSVKMVAAGAEHTAAVTEDGELYGWGWGRYGNLGLGDRNDRL 214
Query: 363 SPMLVTFPHDQKVVQISCGWRHTLAVTERQNVYSWGRGTNGQLGHMESIDRNVPKLIEAL 542
P V+ K+V+++CGWRHT++V+ +Y++G GQLGH + D P +EAL
Sbjct: 215 VPEKVSTVEGVKMVKVACGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLTPHKLEAL 274
Score = 112 bits (280), Expect = 6e-23
Identities = 60/153 (39%), Positives = 90/153 (58%), Gaps = 1/153 (0%)
Frame = +3
Query: 84 KMVLVACGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLIPHKLHALHGELISQISGG 263
+++L++ G H++++ S + ++G + GQLGHGD ED L P L AL G + ++ G
Sbjct: 17 RVLLISAGASHSVALLSGNVVCSWGRGEDGQLGHGDAEDRLSPTYLSALDGHEVVSVTCG 76
Query: 264 WRHTMALT-SNGELYGWGWNKFGQVGVGDDLDHCSPMLVTFPHDQKVVQISCGWRHTLAV 440
HT A + S ++Y WGW FG++G G+ D +P + H ++ QI+CG H LAV
Sbjct: 77 ADHTTAYSESFTQVYSWGWGDFGRLGHGNSSDLFTPQPIKALHGLRIKQIACGDSHCLAV 136
Query: 441 TERQNVYSWGRGTNGQLGHMESIDRNVPKLIEA 539
T V SWGR NGQLG + D VP+ I+A
Sbjct: 137 TMDGEVQSWGRNQNGQLGLGTTEDSLVPQKIQA 169
>emb|CAN67581.1| hypothetical protein VITISV_010346 [Vitis vinifera]
Length = 423
Score = 390 bits (1002), Expect = e-106
Identities = 178/216 (82%), Positives = 196/216 (90%)
Frame = +3
Query: 3 WGRYGNLGLGDRNDRLVPEKVATVDDEKMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
WGRYGNLGLGDRNDRLVPEKV+TV+ KMV VACGWRHTISVSSSGGLYTYGWSKYGQLG
Sbjct: 187 WGRYGNLGLGDRNDRLVPEKVSTVEGVKMVKVACGWRHTISVSSSGGLYTYGWSKYGQLG 246
Query: 183 HGDFEDHLIPHKLHALHGELISQISGGWRHTMALTSNGELYGWGWNKFGQVGVGDDLDHC 362
HGDFEDHL PHKL AL LIS+ISGGWRHTMALTS+G+LYGWGWNKFGQVGVGD++DHC
Sbjct: 247 HGDFEDHLTPHKLEALQENLISEISGGWRHTMALTSDGKLYGWGWNKFGQVGVGDNVDHC 306
Query: 363 SPMLVTFPHDQKVVQISCGWRHTLAVTERQNVYSWGRGTNGQLGHMESIDRNVPKLIEAL 542
SP+ V FPH+QKVV ISCGWRHTLAVTERQNV+SWGRGTNGQLGH ESIDRN+PK+IE L
Sbjct: 307 SPVQVKFPHEQKVVHISCGWRHTLAVTERQNVFSWGRGTNGQLGHGESIDRNIPKMIEVL 366
Query: 543 SVDGSNGQLIESSNIDPSIGKLSVLPSDRYAVVPDD 650
S DGS GQ IE+S +DPS G++ V PS+RYAVVPD+
Sbjct: 367 SADGSGGQQIETSKVDPSTGRIWVSPSERYAVVPDE 402
Score = 138 bits (348), Expect = 8e-31
Identities = 76/223 (34%), Positives = 111/223 (49%), Gaps = 43/223 (19%)
Frame = +3
Query: 3 WGRY--GNLGLGDRNDRLVPEKVATVDDEKMVLVACGWRHTISVSSS------------- 137
WGR G LG GD DRL P ++ +D ++V V CG HT + S S
Sbjct: 40 WGRGEDGQLGHGDAEDRLSPTYLSALDGHEVVSVTCGADHTTAYSESFTQVYSWGCDLFT 99
Query: 138 ----------------------------GGLYTYGWSKYGQLGHGDFEDHLIPHKLHALH 233
G + ++G ++ GQLG G ED L+P K+ A
Sbjct: 100 PQPIKALHGLRIKQIACGDSHCLAVTMDGEVQSWGRNQNGQLGLGTTEDSLVPQKIQAFQ 159
Query: 234 GELISQISGGWRHTMALTSNGELYGWGWNKFGQVGVGDDLDHCSPMLVTFPHDQKVVQIS 413
G + ++ G HT A+T +GELYGWGW ++G +G+GD D P V+ K+V+++
Sbjct: 160 GVSVKMVAAGAEHTAAVTEDGELYGWGWGRYGNLGLGDRNDRLVPEKVSTVEGVKMVKVA 219
Query: 414 CGWRHTLAVTERQNVYSWGRGTNGQLGHMESIDRNVPKLIEAL 542
CGWRHT++V+ +Y++G GQLGH + D P +EAL
Sbjct: 220 CGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLTPHKLEAL 262
Score = 90.1 bits (222), Expect = 3e-16
Identities = 54/153 (35%), Positives = 82/153 (53%), Gaps = 1/153 (0%)
Frame = +3
Query: 84 KMVLVACGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLIPHKLHALHGELISQISGG 263
+++L++ G H++++ + ++G + GQLGHGD ED L P L AL G + ++ G
Sbjct: 17 RVLLISAGASHSVALLXGNVVCSWGRGEDGQLGHGDAEDRLSPTYLSALDGHEVVSVTCG 76
Query: 264 WRHTMALT-SNGELYGWGWNKFGQVGVGDDLDHCSPMLVTFPHDQKVVQISCGWRHTLAV 440
HT A + S ++Y WG + F +P + H ++ QI+CG H LAV
Sbjct: 77 ADHTTAYSESFTQVYSWGCDLF------------TPQPIKALHGLRIKQIACGDSHCLAV 124
Query: 441 TERQNVYSWGRGTNGQLGHMESIDRNVPKLIEA 539
T V SWGR NGQLG + D VP+ I+A
Sbjct: 125 TMDGEVQSWGRNQNGQLGLGTTEDSLVPQKIQA 157
>ref|XP_004168089.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC3-like, partial
[Cucumis sativus]
Length = 302
Score = 384 bits (985), Expect = e-104
Identities = 170/217 (78%), Positives = 198/217 (91%)
Frame = +3
Query: 3 WGRYGNLGLGDRNDRLVPEKVATVDDEKMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
WGRYGNLGLGDRNDRLVP+KV++VD +KMV+VACGWRHTISVSS GGLYTYGWSKYGQLG
Sbjct: 55 WGRYGNLGLGDRNDRLVPQKVSSVDGDKMVMVACGWRHTISVSSLGGLYTYGWSKYGQLG 114
Query: 183 HGDFEDHLIPHKLHALHGELISQISGGWRHTMALTSNGELYGWGWNKFGQVGVGDDLDHC 362
HGDFED L+PH+L AL G+ ISQISGGWRHTMALT++G+LYGWGWNKFGQVG GD++DHC
Sbjct: 115 HGDFEDRLVPHRLEALRGDNISQISGGWRHTMALTTDGKLYGWGWNKFGQVGAGDNIDHC 174
Query: 363 SPMLVTFPHDQKVVQISCGWRHTLAVTERQNVYSWGRGTNGQLGHMESIDRNVPKLIEAL 542
SP+ + FP DQKV+QISCGWRHTLAVT++QNV+SWGRGTNGQLGH ES+DRN P ++EAL
Sbjct: 175 SPIQIKFPQDQKVIQISCGWRHTLAVTDKQNVFSWGRGTNGQLGHGESVDRNTPMILEAL 234
Query: 543 SVDGSNGQLIESSNIDPSIGKLSVLPSDRYAVVPDDN 653
SVDG +GQ IESSNIDPS GK V P++RYA+VPD++
Sbjct: 235 SVDGCSGQQIESSNIDPSSGKTWVSPTERYAIVPDES 271
Score = 124 bits (310), Expect = 2e-26
Identities = 57/133 (42%), Positives = 83/133 (62%)
Frame = +3
Query: 153 YGWSKYGQLGHGDFEDHLIPHKLHALHGELISQISGGWRHTMALTSNGELYGWGWNKFGQ 332
+G ++ GQLG G ED L+P K+ A G I ++ G HT A+T +GEL+GWGW ++G
Sbjct: 1 WGRNQNGQLGLGTTEDSLVPQKIQAFEGISIKMVAAGAEHTAAVTEDGELFGWGWGRYGN 60
Query: 333 VGVGDDLDHCSPMLVTFPHDQKVVQISCGWRHTLAVTERQNVYSWGRGTNGQLGHMESID 512
+G+GD D P V+ K+V ++CGWRHT++V+ +Y++G GQLGH + D
Sbjct: 61 LGLGDRNDRLVPQKVSSVDGDKMVMVACGWRHTISVSSLGGLYTYGWSKYGQLGHGDFED 120
Query: 513 RNVPKLIEALSVD 551
R VP +EAL D
Sbjct: 121 RLVPHRLEALRGD 133
>ref|XP_004146396.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC1-like [Cucumis
sativus]
Length = 438
Score = 384 bits (985), Expect = e-104
Identities = 170/217 (78%), Positives = 198/217 (91%)
Frame = +3
Query: 3 WGRYGNLGLGDRNDRLVPEKVATVDDEKMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
WGRYGNLGLGDRNDRLVP+KV++VD +KMV+VACGWRHTISVSS GGLYTYGWSKYGQLG
Sbjct: 191 WGRYGNLGLGDRNDRLVPQKVSSVDGDKMVMVACGWRHTISVSSLGGLYTYGWSKYGQLG 250
Query: 183 HGDFEDHLIPHKLHALHGELISQISGGWRHTMALTSNGELYGWGWNKFGQVGVGDDLDHC 362
HGDFED L+PH+L AL G+ ISQISGGWRHTMALT++G+LYGWGWNKFGQVG GD++DHC
Sbjct: 251 HGDFEDRLVPHRLEALRGDNISQISGGWRHTMALTTDGKLYGWGWNKFGQVGAGDNIDHC 310
Query: 363 SPMLVTFPHDQKVVQISCGWRHTLAVTERQNVYSWGRGTNGQLGHMESIDRNVPKLIEAL 542
SP+ + FP DQKV+QISCGWRHTLAVT++QNV+SWGRGTNGQLGH ES+DRN P ++EAL
Sbjct: 311 SPIQIKFPQDQKVIQISCGWRHTLAVTDKQNVFSWGRGTNGQLGHGESVDRNTPMILEAL 370
Query: 543 SVDGSNGQLIESSNIDPSIGKLSVLPSDRYAVVPDDN 653
SVDG +GQ IESSNIDPS GK V P++RYA+VPD++
Sbjct: 371 SVDGCSGQQIESSNIDPSSGKTWVSPTERYAIVPDES 407
Score = 157 bits (398), Expect = 1e-36
Identities = 73/183 (39%), Positives = 112/183 (61%)
Frame = +3
Query: 3 WGRYGNLGLGDRNDRLVPEKVATVDDEKMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
WG +G LG G+ +D L P+ + + K+ +ACG H ++V+ G + ++G ++ GQLG
Sbjct: 87 WGDFGRLGHGNSSDLLTPKPIKALHGLKIRQIACGDSHCLAVTMEGEVQSWGRNQNGQLG 146
Query: 183 HGDFEDHLIPHKLHALHGELISQISGGWRHTMALTSNGELYGWGWNKFGQVGVGDDLDHC 362
G ED L+P K+ A G I ++ G HT A+T +GEL+GWGW ++G +G+GD D
Sbjct: 147 LGTTEDSLVPQKIQAFEGISIKMVAAGAEHTAAVTEDGELFGWGWGRYGNLGLGDRNDRL 206
Query: 363 SPMLVTFPHDQKVVQISCGWRHTLAVTERQNVYSWGRGTNGQLGHMESIDRNVPKLIEAL 542
P V+ K+V ++CGWRHT++V+ +Y++G GQLGH + DR VP +EAL
Sbjct: 207 VPQKVSSVDGDKMVMVACGWRHTISVSSLGGLYTYGWSKYGQLGHGDFEDRLVPHRLEAL 266
Query: 543 SVD 551
D
Sbjct: 267 RGD 269
Score = 115 bits (289), Expect = 6e-24
Identities = 63/159 (39%), Positives = 92/159 (57%), Gaps = 1/159 (0%)
Frame = +3
Query: 66 ATVDDEKMVLVACGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLIPHKLHALHGELI 245
AT +++ ++ G H++++ S + ++G + GQLGHGD ED L P +L AL G I
Sbjct: 3 ATSPVRRVLFISAGASHSVALLSGNIVCSWGRGEDGQLGHGDAEDRLSPTQLSALDGHEI 62
Query: 246 SQISGGWRHTMALT-SNGELYGWGWNKFGQVGVGDDLDHCSPMLVTFPHDQKVVQISCGW 422
++ G HT A + + E+Y WGW FG++G G+ D +P + H K+ QI+CG
Sbjct: 63 VSVTCGADHTTAYSVARTEVYSWGWGDFGRLGHGNSSDLLTPKPIKALHGLKIRQIACGD 122
Query: 423 RHTLAVTERQNVYSWGRGTNGQLGHMESIDRNVPKLIEA 539
H LAVT V SWGR NGQLG + D VP+ I+A
Sbjct: 123 SHCLAVTMEGEVQSWGRNQNGQLGLGTTEDSLVPQKIQA 161
>ref|XP_002309939.1| predicted protein [Populus trichocarpa] gi|222852842|gb|EEE90389.1|
predicted protein [Populus trichocarpa]
Length = 444
Score = 379 bits (972), Expect = e-103
Identities = 171/219 (78%), Positives = 194/219 (88%), Gaps = 2/219 (0%)
Frame = +3
Query: 3 WGRYGNLGLGDRNDRLVPEKVATVDDEKMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
WGRYGNLGLGDRNDRLVPEKV+ V+ +KM++VACGWRHTISVSSSGGLYTYGWSKYGQLG
Sbjct: 201 WGRYGNLGLGDRNDRLVPEKVSLVNGDKMIMVACGWRHTISVSSSGGLYTYGWSKYGQLG 260
Query: 183 HGDFEDHLIPHKLHALHGELISQISGGWRHTMALTSNGELYGWGWNKFGQVGVGDDLDHC 362
HGDFEDHL PHK+ AL IS ISGGWRHTMALTS+G LYGWGWNKFGQVGVGD++DHC
Sbjct: 261 HGDFEDHLTPHKVEALRDSYISMISGGWRHTMALTSDGNLYGWGWNKFGQVGVGDNIDHC 320
Query: 363 SPMLVTFPHDQ--KVVQISCGWRHTLAVTERQNVYSWGRGTNGQLGHMESIDRNVPKLIE 536
SP+ V FPH+Q +VQISCGWRHTLA+TERQNV+SWGRGTNGQLGH ES+DRN+PK+IE
Sbjct: 321 SPVQVKFPHEQAHSLVQISCGWRHTLAITERQNVFSWGRGTNGQLGHGESMDRNLPKIIE 380
Query: 537 ALSVDGSNGQLIESSNIDPSIGKLSVLPSDRYAVVPDDN 653
LS DGS G IE+S +DPS+GK V PSDRYA+VPD++
Sbjct: 381 VLSADGSGGHQIEASTVDPSLGKSWVSPSDRYAIVPDES 419
Score = 155 bits (393), Expect = 5e-36
Identities = 69/180 (38%), Positives = 109/180 (60%)
Frame = +3
Query: 3 WGRYGNLGLGDRNDRLVPEKVATVDDEKMVLVACGWRHTISVSSSGGLYTYGWSKYGQLG 182
WG +G LG G+ +D P+ + + K+ +ACG H ++V+ G + ++G ++ GQLG
Sbjct: 97 WGDFGRLGHGNSSDLFTPQPIKALHSLKIRQIACGDSHCLAVTMDGEVQSWGRNQNGQLG 156
Query: 183 HGDFEDHLIPHKLHALHGELISQISGGWRHTMALTSNGELYGWGWNKFGQVGVGDDLDHC 362
G ED L+P K+ A G I ++ G HT A+T +GELYGWGW ++G +G+GD D
Sbjct: 157 LGTTEDSLVPQKIQAFQGVSIKMVAAGAEHTAAVTEDGELYGWGWGRYGNLGLGDRNDRL 216
Query: 363 SPMLVTFPHDQKVVQISCGWRHTLAVTERQNVYSWGRGTNGQLGHMESIDRNVPKLIEAL 542
P V+ + K++ ++CGWRHT++V+ +Y++G GQLGH + D P +EAL
Sbjct: 217 VPEKVSLVNGDKMIMVACGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLTPHKVEAL 276
Score = 110 bits (276), Expect = 2e-22
Identities = 61/153 (39%), Positives = 89/153 (58%), Gaps = 1/153 (0%)
Frame = +3
Query: 84 KMVLVACGWRHTISVSSSGGLYTYGWSKYGQLGHGDFEDHLIPHKLHALHGELISQISGG 263
+++L++ G H++++ S + ++G + GQLGHGD ED P +L L G I I+ G
Sbjct: 19 QVILISAGASHSVALLSGNIVCSWGRGEDGQLGHGDAEDRPTPTQLSTLDGLDIISITCG 78
Query: 264 WRHTMALT-SNGELYGWGWNKFGQVGVGDDLDHCSPMLVTFPHDQKVVQISCGWRHTLAV 440
HT + + S E+Y WGW FG++G G+ D +P + H K+ QI+CG H LAV
Sbjct: 79 ADHTTSYSESRMEVYSWGWGDFGRLGHGNSSDLFTPQPIKALHSLKIRQIACGDSHCLAV 138
Query: 441 TERQNVYSWGRGTNGQLGHMESIDRNVPKLIEA 539
T V SWGR NGQLG + D VP+ I+A
Sbjct: 139 TMDGEVQSWGRNQNGQLGLGTTEDSLVPQKIQA 171